Robert Gordon Miele

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2002 University of Notre Dame, Notre Dame, IN, United States 
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"Robert Miele"
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Francis J. Castellino grad student 2002 Notre Dame
 (Glycosylation of recombinant proteins expressed in lower eukaryotes.)
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Li H, Miele RG, Mitchell TI, et al. (2007) N-linked glycan characterization of heterologous proteins. Methods in Molecular Biology (Clifton, N.J.). 389: 139-50
Bobrowicz P, Davidson RC, Li H, et al. (2004) Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris: production of complex humanized glycoproteins with terminal galactose. Glycobiology. 14: 757-66
Davidson RC, Nett JH, Renfer E, et al. (2004) Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris. Glycobiology. 14: 399-407
Choi BK, Bobrowicz P, Davidson RC, et al. (2003) Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris. Proceedings of the National Academy of Sciences of the United States of America. 100: 5022-7
Miele RG, Prorok M, Costa VA, et al. (1999) Glycosylation of asparagine-28 of recombinant staphylokinase with high-mannose-type oligosaccharides results in a protein with highly attenuated plasminogen activator activity. The Journal of Biological Chemistry. 274: 7769-76
Duman JG, Miele RG, Liang H, et al. (1998) O-Mannosylation of Pichia pastoris cellular and recombinant proteins. Biotechnology and Applied Biochemistry. 28: 39-45
Miele RG, Castellino FJ, Bretthauer RK. (1997) Characterization of the acidic oligosaccharides assembled on the Pichia pastoris-expressed recombinant kringle 2 domain of human tissue-type plasminogen activator. Biotechnology and Applied Biochemistry. 26: 79-83
Miele RG, Nilsen SL, Brito T, et al. (1997) Glycosylation properties of the Pichia pastoris-expressed recombinant kringle 2 domain of tissue-type plasminogen activator. Biotechnology and Applied Biochemistry. 25: 151-7
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