Satoshi Inouye, Ph.D. - Publications

Affiliations: 
1984-2022 Chisso Corp. JNC Corp. 
Area:
Bioluminescence

76 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2022 Sugimoto H, Fukuda S, Yokawa S, Hori M, Ninomiya H, Sato T, Miyazawa K, Kawai T, Furuno T, Inouye S, Goto S, Suzuki T. Visualization of osteocalcin and bone morphogenetic protein 2 (BMP2) secretion from osteoblastic cells by bioluminescence imaging. Biochemical and Biophysical Research Communications. 635: 203-209. PMID 36279682 DOI: 10.1016/j.bbrc.2022.10.042  0.325
2022 Inouye S, Sato JI, Sahara-Miura Y, Tomabechi Y, Sumida Y, Sekine SI, Shirouzu M, Hosoya T. Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate. Plos One. 17: e0272992. PMID 36129943 DOI: 10.1371/journal.pone.0272992  0.379
2022 Inouye S, Nakamura M, Hosoya T. Enzymatic conversion of dehydrocoelenterazine to coelenterazine using FMN-bound flavin reductase of NAD(P)H:FMN oxidoreductase. Biochemical and Biophysical Research Communications. 587: 24-28. PMID 34864391 DOI: 10.1016/j.bbrc.2021.11.089  0.314
2021 Inouye S, Nakamura M, Hosoya T. Formation of Coelenteramine from 2-Peroxycoelenterazine in the Ca -Binding Photoprotein Aequorin. Photochemistry and Photobiology. PMID 34971002 DOI: 10.1111/php.13590  0.366
2021 Inouye S. Multiple Cypridina Luciferase Genes in the Genome of Individual Ostracods, Vargula hilgendorfii (Cypridina hilgendorfii). Photochemistry and Photobiology. PMID 34181758 DOI: 10.1111/php.13479  0.27
2021 Inouye S, Sumida Y, Tomabechi Y, Taguchi J, Shirouzu M, Hosoya T. Chiral deaza-coelenterazine analogs for probing a substrate-binding site in the Ca2+-binding photoprotein aequorin. Plos One. 16: e0251743. PMID 34115795 DOI: 10.1371/journal.pone.0251743  0.319
2020 Suzuki T, Kanamori T, Inouye S. [Novel Technology for Studying Insulin Secretion: Imaging and Quantitative Analysis by a Bioluminescence Method]. Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan. 140: 969-977. PMID 32741870 DOI: 10.1248/yakushi.20-00012-2  0.285
2020 Nakamura M, Matsuda K, Morikawa T, Ishizuka K, Inouye S. Efficient conversion to Cypridina luciferin from Cypridina luciferyl sulfate, coupled with enzymatic sulfation of acetic acid. Biochemical and Biophysical Research Communications. 529: 678-684. PMID 32736692 DOI: 10.1016/j.bbrc.2020.05.167  0.319
2020 Inouye S, Nakamura M, Taguchi J, Hosoya T. Identification of a novel oxidation product from yellow fluorophore in the complex of apoPholasin and dehydrocoelenterazine. Bioorganic & Medicinal Chemistry Letters. 127435. PMID 32717370 DOI: 10.1016/j.bmcl.2020.127435  0.324
2020 Inouye S, Miura-Sahara Y, Iimori R, Sakata Y, Hazama Y, Yoshida S, Nakamura M, Hosoya T. A novel yellow fluorescent protein of recombinant apoPholasin with dehydrocoelenterazine. Biochemical and Biophysical Research Communications. PMID 32223929 DOI: 10.1016/j.bbrc.2020.03.085  0.343
2020 Inouye S, Sahara-Miura Y, Nakamura M, Hosoya T. Expression, purification, and characterization of recombinant apoPholasin. Protein Expression and Purification. 105615. PMID 32114101 DOI: 10.1016/j.pep.2020.105615  0.375
2019 Nakamura M, Matsuda K, Nakamura M, Yamashita K, Suzuki T, Inouye S. Enzymatic Conversion of Cypridina Luciferyl Sulfate to Cypridina Luciferin with Coenzyme A as a Sulfate Acceptor in Cypridina (Vargula) hilgendorfii. Photochemistry and Photobiology. PMID 31230356 DOI: 10.1111/php.13137  0.309
2018 Inouye S, Hojo H. Revalidation of recombinant aequorin as a light emission standard: Estimation of specific activity of Gaussia luciferase. Biochemical and Biophysical Research Communications. PMID 30424878 DOI: 10.1016/j.bbrc.2018.11.015  0.353
2018 Yokawa S, Suzuki T, Hayashi A, Inouye S, Inoh Y, Furuno T. Video-Rate Bioluminescence Imaging of Degranulation of Mast Cells Attached to the Extracellular Matrix. Frontiers in Cell and Developmental Biology. 6: 74. PMID 30042943 DOI: 10.3389/fcell.2018.00074  0.322
2018 Inouye S, Tomabechi Y, Hosoya T, Sekine SI, Shirouzu M. Slow luminescence kinetics of semi-synthetic aequorin: Expression, purification and structure determination of cf3-aequorin. Journal of Biochemistry. PMID 29796619 DOI: 10.1093/jb/mvy049  0.282
2017 Inouye S. Single-step purification of recombinant Gaussia luciferase from serum-containing culture medium of mammalian cells. Protein Expression and Purification. PMID 28888757 DOI: 10.1016/j.pep.2017.09.001  0.335
2017 Inouye S, Sahara-Miura Y. A fusion protein of the synthetic IgG-binding domain and aequorin: Expression and purification from E. coli cells and its application. Protein Expression and Purification. PMID 28668497 DOI: 10.1016/j.pep.2017.06.017  0.383
2017 Suzuki T, Kanamori T, Inouye S. Quantitative visualization of synchronized insulin secretion from 3D-cultured cells. Biochemical and Biophysical Research Communications. PMID 28342877 DOI: 10.1016/j.bbrc.2017.03.105  0.258
2017 Yokawa S, Suzuki T, Inouye S, Inoh Y, Suzuki R, Kanamori T, Furuno T, Hirashima N. Visualization of glucagon secretion from pancreatic α cells by bioluminescence video microscopy: Identification of secretion sites in the intercellular contact regions. Biochemical and Biophysical Research Communications. PMID 28238783 DOI: 10.1016/j.bbrc.2017.02.114  0.313
2016 Inouye S, Suzuki T. Protein expression of preferred human codon-optimized Gaussia luciferase genes with an artificial open-reading frame in mammalian and bacterial cells. Protein Expression and Purification. PMID 27506135 DOI: 10.1016/j.pep.2016.08.006  0.34
2016 Inouye S, Sahara-Miura Y. Expression and characterization of EF-hand I loop mutants of aequorin replaced with other loop sequences of Ca2+-binding proteins: an approach to studying the EF-hand motif of proteins. Journal of Biochemistry. 160: 59-68. PMID 26896488 DOI: 10.1093/jb/mvw013  0.356
2016 Inouye S, Sahara-Miura Y. Identification of amino acid residues responsible for high initial luminescence intensity in a calcium-binding photoprotein, clytin-II. Biochemical and Biophysical Research Communications. 469: 300-5. PMID 26626073 DOI: 10.1016/j.bbrc.2015.11.097  0.309
2015 Tomabechi Y, Hosoya T, Ehara H, Sekine SI, Shirouzu M, Inouye S. Crystal structure of nanoKAZ: the mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine. Biochemical and Biophysical Research Communications. PMID 26746005 DOI: 10.1016/j.bbrc.2015.12.123  0.259
2015 Hosoya T, Iimori R, Yoshida S, Sumida Y, Sahara-Miura Y, Sato J, Inouye S. Concise Synthesis of v-Coelenterazines. Organic Letters. 17: 3888-91. PMID 26194904 DOI: 10.1021/acs.orglett.5b01872  0.265
2015 Inouye S, Sahara-Miura Y, Sato J, Suzuki T. Codon optimization of genes for efficient protein expression in mammalian cells by selection of only preferred human codons. Protein Expression and Purification. 109: 47-54. PMID 25665506 DOI: 10.1016/j.pep.2015.02.002  0.307
2014 Inouye S, Sato J, Sahara-Miura Y, Hosoya T, Suzuki T. Unconventional secretion of the mutated 19 kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells. Biochemical and Biophysical Research Communications. 450: 1313-9. PMID 25019994 DOI: 10.1016/j.bbrc.2014.06.140  0.314
2014 Suzuki T, Inouye S. [Visualization of protein secretion by video-rate bioluminescence imaging]. Seikagaku. the Journal of Japanese Biochemical Society. 86: 281-5. PMID 24864458  0.261
2014 Inouye S, Sato J, Sahara-Miura Y, Yoshida S, Hosoya T. Luminescence enhancement of the catalytic 19 kDa protein (KAZ) of Oplophorus luciferase by three amino acid substitutions. Biochemical and Biophysical Research Communications. 445: 157-62. PMID 24491536 DOI: 10.1016/j.bbrc.2014.01.133  0.357
2014 Suzuki T, Inouye S. Video-rate bioluminescence imaging of protein secretion from a living cell. Methods in Molecular Biology (Clifton, N.J.). 1098: 71-83. PMID 24166369 DOI: 10.1007/978-1-62703-718-1_6  0.347
2013 Oba Y, Yoshida N, Kanie S, Ojika M, Inouye S. Biosynthesis of firefly luciferin in adult lantern: decarboxylation of L-cysteine is a key step for benzothiazole ring formation in firefly luciferin synthesis. Plos One. 8: e84023. PMID 24391868 DOI: 10.1371/Journal.Pone.0084023  0.327
2013 Inouye S, Sahara-Miura Y. A Novel Catalytic Function of Synthetic IgG-Binding Domain (Z Domain) from Staphylococcal Protein A: Light Emission with Coelenterazine. Photochemistry and Photobiology. 90: 137-44. PMID 24138575 DOI: 10.1111/php.12192  0.337
2013 Inouye S, Sato J, Sahara-Miura Y, Yoshida S, Kurakata H, Hosoya T. C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: the mutated catalytic 19 kDa component of Oplophorus luciferase. Biochemical and Biophysical Research Communications. 437: 23-8. PMID 23792095 DOI: 10.1016/j.bbrc.2013.06.026  0.292
2013 Oba Y, Furuhashi M, Bessho M, Sagawa S, Ikeya H, Inouye S. Bioluminescence of a firefly pupa: involvement of a luciferase isotype in the dim glow of pupae and eggs in the Japanese firefly, Luciola lateralis. Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society For Photobiology. 12: 854-63. PMID 23407656 DOI: 10.1039/c3pp25363e  0.292
2013 Inouye S, Sahara-Miura Y, Sato J, Iimori R, Yoshida S, Hosoya T. Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines. Protein Expression and Purification. 88: 150-6. PMID 23274053 DOI: 10.1016/j.pep.2012.12.006  0.29
2012 Inouye S, Sato J. Purification of histidine-tagged aequorin with a reactive cysteine residue for chemical conjugations and its application for bioluminescent sandwich immunoassays. Protein Expression and Purification. 83: 205-10. PMID 22538315 DOI: 10.1016/j.pep.2012.04.001  0.365
2012 Oba Y, Yoshida M, Shintani T, Furuhashi M, Inouye S. Firefly luciferase genes from the subfamilies Psilocladinae and Ototretinae (Lampyridae, Coleoptera). Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology. 161: 110-6. PMID 22008901 DOI: 10.1016/j.cbpb.2011.10.001  0.315
2011 Suzuki T, Kondo C, Kanamori T, Inouye S. Video-rate bioluminescence imaging of matrix metalloproteinase-2 secreted from a migrating cell Plos One. 6. PMID 21969874 DOI: 10.1371/journal.pone.0025243  0.262
2011 Kitai Y, Fukuda H, Enomoto T, Asakawa Y, Suzuki T, Inouye S, Handa H. Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor. Journal of Biotechnology. 155: 251-6. PMID 21745502 DOI: 10.1016/J.Jbiotec.2011.06.030  0.416
2011 Inouye S, Sato J, Sahara-Miura Y. Recombinant Gaussia luciferase with a reactive cysteine residue for chemical conjugation: expression, purification and its application for bioluminescent immunoassays. Biochemical and Biophysical Research Communications. 410: 792-7. PMID 21703234 DOI: 10.1016/j.bbrc.2011.06.063  0.369
2011 Suzuki T, Kondo C, Kanamori T, Inouye S. Video rate bioluminescence imaging of secretory proteins in living cells: Localization, secretory frequency, and quantification Analytical Biochemistry. 415: 182-189. PMID 21477579 DOI: 10.1016/j.ab.2011.03.039  0.295
2011 Inouye S, Sato J, Sasaki S, Sahara Y. Streptavidin-aequorin fusion protein for bioluminescent immunoassay. Bioscience, Biotechnology, and Biochemistry. 75: 568-71. PMID 21389603 DOI: 10.1271/bbb.100798  0.301
2010 Oba Y, Mori N, Yoshida M, Inouye S. Identification and characterization of a luciferase isotype in the Japanese firefly, Luciola cruciata, involving in the dim glow of firefly eggs. Biochemistry. 49: 10788-95. PMID 21090686 DOI: 10.1021/bi1016342  0.287
2010 Inouye S, Iimori R, Sahara Y, Hisada S, Hosoya T. Application of new semisynthetic aequorins with long half-decay time of luminescence to G-protein-coupled receptor assay. Analytical Biochemistry. 407: 247-52. PMID 20800051 DOI: 10.1016/j.ab.2010.08.025  0.265
2009 Oba Y, Kumazaki M, Inouye S. Characterization of luciferases and its paralogue in the Panamanian luminous click beetle Pyrophorus angustus: a click beetle luciferase lacks the fatty acyl-CoA synthetic activity. Gene. 452: 1-6. PMID 20004235 DOI: 10.1016/j.gene.2009.12.001  0.327
2009 Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cellular and Molecular Life Sciences : Cmls. 67: 387-404. PMID 19859663 DOI: 10.1007/s00018-009-0170-8  0.33
2009 Oba Y, Kato S, Ojika M, Inouye S. Biosynthesis of coelenterazine in the deep-sea copepod, Metridia pacifica. Biochemical and Biophysical Research Communications. 390: 684-8. PMID 19833098 DOI: 10.1016/J.Bbrc.2009.10.028  0.266
2009 Inouye S, Hosoya T. Reconstitution of blue fluorescent protein from recombinant apoaequorin and synthetic coelenteramide Biochemical and Biophysical Research Communications. 386: 617-622. PMID 19549504 DOI: 10.1016/j.bbrc.2009.06.094  0.373
2009 Oba Y, Iida K, Inouye S. Functional conversion of fatty acyl-CoA synthetase to firefly luciferase by site-directed mutagenesis: a key substitution responsible for luminescence activity. Febs Letters. 583: 2004-8. PMID 19450587 DOI: 10.1016/j.febslet.2009.05.018  0.334
2009 Inouye S, Sahara Y. Expression and purification of the calcium binding photoprotein mitrocomin using ZZ-domain as a soluble partner in E. coli cells. Protein Expression and Purification. 66: 52-7. PMID 19367679 DOI: 10.1016/j.pep.2009.01.010  0.371
2008 Inouye S, Sato J. Comparison of luminescent immunoassays using biotinylated proteins of aequorin, alkaline phosphatase and horseradish peroxidase as reporters. Bioscience, Biotechnology, and Biochemistry. 72: 3310-3. PMID 19060386 DOI: 10.1271/bbb.80524  0.327
2008 Inouye S, Sahara Y. Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system. Biochemical and Biophysical Research Communications. 376: 448-53. PMID 18789309 DOI: 10.1016/j.bbrc.2008.08.149  0.354
2008 Oba Y, Shintani T, Nakamura T, Ojika M, Inouye S. Determination of the luciferin contents in luminous and non-luminous beetles. Bioscience, Biotechnology, and Biochemistry. 72: 1384-7. PMID 18460798 DOI: 10.1271/Bbb.80017  0.287
2008 Inouye S, Sato J. Recombinant aequorin with a reactive cysteine residue for conjugation with maleimide-activated antibody. Analytical Biochemistry. 378: 105-7. PMID 18417074 DOI: 10.1016/j.ab.2008.03.044  0.283
2008 Inouye S. Cloning, expression, purification and characterization of an isotype of clytin, a calcium-binding photoprotein from the luminous hydromedusa Clytia gregarium. Journal of Biochemistry. 143: 711-7. PMID 18296715 DOI: 10.1093/jb/mvn024  0.317
2008 Suzuki M, Furukawa S, Kuramori C, Sawa C, Kabe Y, Nakamura M, Sawada J, Yamaguchi Y, Sakamoto S, Inouye S, Handa H. Development of a chemical screening system using aqueorin-fused protein. Biochemical and Biophysical Research Communications. 368: 600-5. PMID 18243129 DOI: 10.1016/J.Bbrc.2008.01.107  0.439
2008 Oba Y, Iida K, Ojika M, Inouye S. Orthologous gene of beetle luciferase in non-luminous click beetle, Agrypnus binodulus (Elateridae), encodes a fatty acyl-CoA synthetase. Gene. 407: 169-75. PMID 17996401 DOI: 10.1016/J.Gene.2007.10.013  0.336
2007 Inouye S, Sahara Y. Identification of two catalytic domains in a luciferase secreted by the copepod Gaussia princeps. Biochemical and Biophysical Research Communications. 365: 96-101. PMID 17981153 DOI: 10.1016/j.bbrc.2007.10.152  0.339
2007 Inouye S, Sasaki S. Overexpression, purification and characterization of the catalytic component of Oplophorus luciferase in the deep-sea shrimp, Oplophorus gracilirostris. Protein Expression and Purification. 56: 261-8. PMID 17900925 DOI: 10.1016/j.pep.2007.08.002  0.388
2007 Suzuki T, Usuda S, Ichinose H, Inouye S. Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. Febs Letters. 581: 4551-6. PMID 17825828 DOI: 10.1016/J.Febslet.2007.08.036  0.327
2007 Ito K, Nishimura W, Maeda M, Gomi K, Inouye S, Arakawa H. Highly sensitive and rapid tandem bioluminescent immunoassay using aequorin labeled Fab fragment and biotinylated firefly luciferase. Analytica Chimica Acta. 588: 245-51. PMID 17386817 DOI: 10.1016/j.aca.2007.02.005  0.279
2007 Inouye S, Sasaki S. Imidazole-assisted catalysis of luminescence reaction in blue fluorescent protein from the photoprotein aequorin. Biochemical and Biophysical Research Communications. 354: 650-5. PMID 17254548 DOI: 10.1016/j.bbrc.2006.12.233  0.392
2006 Inouye S, Sahara Y. Expression, purification and characterization of a photoprotein, clytin, from Clytia gregarium. Protein Expression and Purification. 53: 384-9. PMID 17275329 DOI: 10.1016/j.pep.2006.12.014  0.38
2006 Oba Y, Tanaka K, Inouye S. Catalytic properties of domain-exchanged chimeric proteins between firefly luciferase and Drosophila fatty Acyl-CoA synthetase CG6178. Bioscience, Biotechnology, and Biochemistry. 70: 2739-44. PMID 17090919 DOI: 10.1271/bbb.60364  0.333
2006 Inouye S. Expression, purification and characterization of calcium-triggered luciferin-binding protein of Renilla reniformis. Protein Expression and Purification. 52: 66-73. PMID 16997571 DOI: 10.1016/j.pep.2006.07.028  0.418
2006 Kato S, Oba Y, Ojika M, Inouye S. Stereoselective incorporation of isoleucine into Cypridina luciferin in Cypridina hilgendorfii (Vargula hilgendorfii). Bioscience, Biotechnology, and Biochemistry. 70: 1528-32. PMID 16794342 DOI: 10.1271/Bbb.60066  0.255
2006 Inouye S, Sasaki S. Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase. Febs Letters. 580: 1977-82. PMID 16545379 DOI: 10.1016/j.febslet.2006.02.065  0.376
2006 Ohashi W, Inouye S, Yamazaki T, Hirota H. NMR analysis of the Mg2+-binding properties of aequorin, a Ca2+-binding photoprotein. Journal of Biochemistry. 138: 613-20. PMID 16272573 DOI: 10.1093/jb/mvi164  0.28
2005 Oba Y, Sato M, Ohta Y, Inouye S. Identification of paralogous genes of firefly luciferase in the Japanese firefly, Luciola cruciata. Gene. 368: 53-60. PMID 16380223 DOI: 10.1016/j.gene.2005.10.023  0.303
2005 Ohashi W, Inouye S, Yamazaki T, Doi-Katayama Y, Yokoyama S, Hirota H. Backbone 1H, 13C and 15N resonance assignments for the Mg2+-bound form of the Ca2+-binding photoprotein aequorin. Journal of Biomolecular Nmr. 31: 375-6. PMID 15929011 DOI: 10.1007/s10858-005-1609-3  0.272
2005 Oba Y, Sato M, Ojika M, Inouye S. Enzymatic and genetic characterization of firefly luciferase and Drosophila CG6178 as a fatty acyl-CoA synthetase. Bioscience, Biotechnology, and Biochemistry. 69: 819-28. PMID 15849423 DOI: 10.1271/Bbb.69.819  0.323
2005 Toma S, Chong KT, Nakagawa A, Teranishi K, Inouye S, Shimomura O. The crystal structures of semi-synthetic aequorins. Protein Science : a Publication of the Protein Society. 14: 409-16. PMID 15632284 DOI: 10.1107/S0108767302089596  0.583
2005 Inouye S. Blue fluorescent protein from the calcium-sensitive photoprotein aequorin is a heat resistant enzyme, catalyzing the oxidation of coelenterazine. Febs Letters. 577: 105-10. PMID 15527769 DOI: 10.1016/j.febslet.2004.09.078  0.353
2004 Oba Y, Tsuduki H, Kato S, Ojika M, Inouye S. Identification of the luciferin-luciferase system and quantification of coelenterazine by mass spectrometry in the deep-sea luminous ostracod Conchoecia pseudodiscophora. Chembiochem : a European Journal of Chemical Biology. 5: 1495-9. PMID 15515099 DOI: 10.1002/Cbic.200400102  0.285
2004 Oba Y, Ojika M, Inouye S. Characterization of CG6178 gene product with high sequence similarity to firefly luciferase in Drosophila melanogaster. Gene. 329: 137-45. PMID 15033536 DOI: 10.1016/J.Gene.2003.12.026  0.329
2003 Inouye S, Nakamura M. Identification of biotinylated lysine residues in the photoprotein aequorin by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry peptide mapping after lysine-specific endopeptidase digestion. Analytical Biochemistry. 316: 216-22. PMID 12711343 DOI: 10.1016/s0003-2697(03)00055-1  0.272
2003 Oba Y, Ojika M, Inouye S. Firefly luciferase is a bifunctional enzyme: ATP-dependent monooxygenase and a long chain fatty acyl-CoA synthetase. Febs Letters. 540: 251-4. PMID 12681517 DOI: 10.1016/S0014-5793(03)00272-2  0.318
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