John C. Haugner, Ph.D.

Affiliations: 
2014 Biochemistry, Molecular Bio, and Biophysics University of Minnesota, Twin Cities, Minneapolis, MN 
Area:
Biochemistry, Molecular Biology, Cell Biology
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"John Haugner"
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Burckhard Seelig grad student 2014 UMN
 (In vitro evolution of artificial enzymes: Method development and applications.)
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Publications

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Shan Q, Baltes NJ, Atkins P, et al. (2018) ZFN, TALEN and CRISPR-Cas9 mediated homology directed gene insertion in Arabidopsis: A disconnect between somatic and germinal cells. Journal of Genetics and Genomics = Yi Chuan Xue Bao
Morelli A, Haugner J, Seelig B. (2014) Thermostable artificial enzyme isolated by in vitro selection. Plos One. 9: e112028
Morelli A, Haugner J, Seelig B. (2014) Sequence differences between ligase #7 and ligase 10C mapped onto the NMR structure of ligase 10C [20] . Plos One
Morelli A, Haugner J, Seelig B. (2014) Thermal unfolding curves of ligases #6, #7 and 10C. Plos One
Morelli A, Haugner J, Seelig B. (2014) Activity of ligase enzymes assayed at different temperatures. Plos One
Morelli A, Haugner J, Seelig B. (2014) In vitro selection of artificial ligase enzymes with increased stability. Plos One
Golynskiy MV, Haugner JC, Seelig B. (2013) Highly diverse protein library based on the ubiquitous (β/α)₈ enzyme fold yields well-structured proteins through in vitro folding selection. Chembiochem : a European Journal of Chemical Biology. 14: 1553-63
Haugner JC, Seelig B. (2013) Universal labeling of 5'-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity. Chemical Communications (Cambridge, England). 49: 7322-4
Golynskiy MV, Haugner JC, Morelli A, et al. (2013) In vitro evolution of enzymes. Methods in Molecular Biology (Clifton, N.J.). 978: 73-92
Chao FA, Morelli A, Haugner JC, et al. (2013) Structure and dynamics of a primordial catalytic fold generated by in vitro evolution. Nature Chemical Biology. 9: 81-3
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