Kevin T. Boulware, Ph.D.

Affiliations: 
2007 Chemical Engineering University of California, Santa Barbara, Santa Barbara, CA, United States 
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Patrick S. Daugherty grad student 2007 UC Santa Barbara
 (Cellular libraries of peptide substrates (CLiPS): A high-throughput method for proteolytic enzyme characterization.)
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Boulware KT, Jabaiah A, Daugherty PS. (2010) Evolutionary optimization of peptide substrates for proteases that exhibit rapid hydrolysis kinetics. Biotechnology and Bioengineering. 106: 339-46
Heinzelman P, Snow CD, Smith MA, et al. (2009) SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability. The Journal of Biological Chemistry. 284: 26229-33
Dubin G, Stec-Niemczyk J, Kisielewska M, et al. (2008) Enzymatic activity of the Staphylococcus aureus SplB serine protease is induced by substrates containing the sequence Trp-Glu-Leu-Gln. Journal of Molecular Biology. 379: 343-56
Boulware KT, Daugherty PS. (2006) Protease specificity determination by using cellular libraries of peptide substrates (CLiPS). Proceedings of the National Academy of Sciences of the United States of America. 103: 7583-8
Rice JJ, Schohn A, Bessette PH, et al. (2006) Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands. Protein Science : a Publication of the Protein Society. 15: 825-36
Boulware KT, Daugherty PS. (2005) Bacterial substrate display: A new method for profiling protease activity Aiche Annual Meeting, Conference Proceedings. 9268
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