Carl T. Wittwer - Publications

Bioengineering University of Utah, Salt Lake City, UT 
Biomedical Engineering

167 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2015 Rifai N, Topol E, Chan E, Lo YM, Wittwer CT. Disruptive Innovation in Laboratory Medicine. Clinical Chemistry. 61: 1129-32. PMID 26152753 DOI: 10.1373/clinchem.2015.243667  0.92
2015 Liew M, Mao R, Wittwer CT, Salama ME. Detection of chromosomal translocations in formalin-fixed paraffin-embedded (FFPE) leukemic specimens by digital expression profiling. International Journal of Laboratory Hematology. 37: 690-8. PMID 26037935 DOI: 10.1111/ijlh.12388  0.92
2015 Zhou L, Palais RA, Paxton CN, Geiersbach KB, Wittwer CT. Copy number assessment by competitive PCR with limiting deoxynucleotide triphosphates and high-resolution melting. Clinical Chemistry. 61: 724-33. PMID 25759466 DOI: 10.1373/clinchem.2014.236208  0.92
2015 Liew M, Farley M, Andreasen J, Parker CJ, Wittwer CT. Rare event counting of CD59- red cells in human blood: A 47-month experience using PNH consensus guidelines for WBC and RBC testing in a reference lab. Cytometry. Part B, Clinical Cytometry. 88: 261-9. PMID 25605539 DOI: 10.1002/cyto.b.21225  0.92
2015 Chiu RW, Lo YM, Wittwer CT. Molecular diagnostics: a revolution in progress. Clinical Chemistry. 61: 1-3. PMID 25550473 DOI: 10.1373/clinchem.2014.233361  0.92
2015 Farrar JS, Wittwer CT. Extreme PCR: efficient and specific DNA amplification in 15-60 seconds. Clinical Chemistry. 61: 145-53. PMID 25320377 DOI: 10.1373/clinchem.2014.228304  0.92
2014 Sundberg SO, Wittwer CT, Howell RM, Huuskonen J, Pryor RJ, Farrar JS, Stiles HM, Palais RA, Knight IT. Microfluidic genotyping by rapid serial PCR and high-speed melting analysis. Clinical Chemistry. 60: 1306-13. PMID 25117377 DOI: 10.1373/clinchem.2014.223768  0.92
2014 Sundberg SO, Wittwer CT, Zhou L, Palais R, Dwight Z, Gale BK. Quasi-digital PCR: Enrichment and quantification of rare DNA variants. Biomedical Microdevices. 16: 639-44. PMID 24781884 DOI: 10.1007/s10544-014-9866-0  0.92
2014 Li M, Zhou L, Palais RA, Wittwer CT. Genotyping accuracy of high-resolution DNA melting instruments. Clinical Chemistry. 60: 864-72. PMID 24718912 DOI: 10.1373/clinchem.2013.220160  0.92
2014 Montgomery JL, Rejali N, Wittwer CT. The influence of nucleotide sequence and temperature on the activity of thermostable DNA polymerases. The Journal of Molecular Diagnostics : Jmd. 16: 305-13. PMID 24607271 DOI: 10.1016/j.jmoldx.2014.01.006  0.92
2014 Dwight ZL, Palais R, Kent J, Wittwer CT. Heterozygote PCR product melting curve prediction. Human Mutation. 35: 278-82. PMID 24375593 DOI: 10.1002/humu.22494  0.92
2014 Sanford LN, Wittwer CT. Fluorescence-based temperature control for polymerase chain reaction. Analytical Biochemistry. 448: 75-81. PMID 24291705 DOI: 10.1016/j.ab.2013.11.027  0.92
2014 Montgomery JL, Wittwer CT. Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments. Clinical Chemistry. 60: 334-40. PMID 24081987 DOI: 10.1373/clinchem.2013.212829  0.92
2013 Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, et al. The need for transparency and good practices in the qPCR literature. Nature Methods. 10: 1063-7. PMID 24173381 DOI: 10.1038/nmeth.2697  0.92
2013 Sanford LN, Kent JO, Wittwer CT. Quantum method for fluorescence background removal in DNA melting analysis. Analytical Chemistry. 85: 9907-15. PMID 24070125 DOI: 10.1021/ac4024928  0.92
2013 Wittwer CT, Herrmann MG, Moss AA, Rasmussen RP. Continuous fluorescence monitoring of rapid cycle DNA amplification. 1997. Biotechniques. 54: 314-20. PMID 23905170 DOI: 10.2144/000114043  0.92
2013 Montgomery JL, Rejali N, Wittwer CT. Stopped-flow DNA polymerase assay by continuous monitoring of dNTP incorporation by fluorescence. Analytical Biochemistry. 441: 133-9. PMID 23872003 DOI: 10.1016/j.ab.2013.07.008  0.92
2013 Illson ML, Dempsey-Nunez L, Kent J, Huang Q, Brebner A, Raff ML, Watkins D, Gilfix BM, Wittwer CT, Rosenblatt DS. High resolution melting analysis of the MMAB gene in cblB patients and in those with undiagnosed methylmalonic aciduria. Molecular Genetics and Metabolism. 110: 86-9. PMID 23707710 DOI: 10.1016/j.ymgme.2013.04.020  0.92
2013 Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA. The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments. Clinical Chemistry. 59: 892-902. PMID 23570709 DOI: 10.1373/clinchem.2013.206375  0.92
2013 Zhou L, Palais RA, Ye F, Chen J, Montgomery JL, Wittwer CT. Symmetric snapback primers for scanning and genotyping of the cystic fibrosis transmembrane conductance regulator gene. Clinical Chemistry. 59: 1052-61. PMID 23503723 DOI: 10.1373/clinchem.2013.202689  0.92
2013 Sanford LN, Wittwer CT. Monitoring temperature with fluorescence during real-time PCR and melting analysis. Analytical Biochemistry. 434: 26-33. PMID 23142429 DOI: 10.1016/j.ab.2012.10.037  0.92
2012 Dempsey-Nunez L, Illson ML, Kent J, Huang Q, Brebner A, Watkins D, Gilfix BM, Wittwer CT, Rosenblatt DS. High resolution melting analysis of the MMAA gene in patients with cblA and in those with undiagnosed methylmalonic aciduria. Molecular Genetics and Metabolism. 107: 363-7. PMID 23026888 DOI: 10.1016/j.ymgme.2012.09.012  0.92
2012 Dwight ZL, Palais R, Wittwer CT. uAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictions. Ieee/Acm Transactions On Computational Biology and Bioinformatics / Ieee, Acm. 9: 1805-11. PMID 22889837 DOI: 10.1109/TCBB.2012.112  0.92
2012 Rifai N, Diamandis EP, Lo YM, Kricka LJ, Wilding P, Ladenson JH, Wittwer CT. Advancing laboratory medicine through innovation: a tale of six inventors. Clinical Chemistry. 58: 502-10. PMID 22238255 DOI: 10.1373/clinchem.2011.178582  0.92
2011 Smith GD, Zhou L, Rowe LR, Jarboe EA, Collins BT, Bentz JS, Wittwer CT, Chadwick BE. Allele-specific PCR with competitive probe blocking for sensitive and specific detection of BRAF V600E in thyroid fine-needle aspiration specimens. Acta Cytologica. 55: 576-83. PMID 22156469 DOI: 10.1159/000333453  0.92
2011 von Ahsen N, Wittwer CT, Schütz E. Monovalent and divalent salt correction algorithms for Tm prediction--recommendations for Primer3 usage. Briefings in Bioinformatics. 12: 514-7. PMID 21949267 DOI: 10.1093/bib/bbq081  0.92
2011 von Kanel T, Gerber D, Wittwer CT, Hermann M, Gallati S. Detecting and resolving position-dependent temperature effects in real-time quantitative polymerase chain reaction. Analytical Biochemistry. 419: 161-7. PMID 21925477 DOI: 10.1016/j.ab.2011.08.041  0.92
2011 Farrar JS, Palais RA, Wittwer CT. Snapback primer genotyping of the Gilbert syndrome UGT1A1 (TA)(n) promoter polymorphism by high-resolution melting. Clinical Chemistry. 57: 1303-10. PMID 21771946 DOI: 10.1373/clinchem.2011.166306  0.92
2011 Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. Primer sequence disclosure: a clarification of the MIQE guidelines. Clinical Chemistry. 57: 919-21. PMID 21421813 DOI: 10.1373/clinchem.2011.162958  0.92
2011 Dwight Z, Palais R, Wittwer CT. uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application. Bioinformatics (Oxford, England). 27: 1019-20. PMID 21300699 DOI: 10.1093/bioinformatics/btr065  0.92
2011 Seipp MT, Wittwer CT. Detecting rare thrombophilia variants by high-resolution melting. Clinical Chemistry. 57: 639-41. PMID 21292852 DOI: 10.1373/clinchem.2010.159160  0.92
2011 Dobrowolski SF, Wittwer CT. High-Resolution Melt Profiling Molecular Analysis and Genome Discovery: Second Edition. 81-113. DOI: 10.1002/9781119977438.ch5  0.92
2011 Wittwer CT. Evolution of polymerase chain reaction: Seminal technology continues to be a work in progress Genetic Engineering and Biotechnology News. 31.  0.92
2010 Seipp MT, Herrmann M, Wittwer CT. Automated DNA extraction, quantification, dilution, and PCR preparation for genotyping by high-resolution melting. Journal of Biomolecular Techniques : Jbt. 21: 163-6. PMID 21119928  0.92
2010 Wittwer CT. Making DNA melting useful. Clinical Chemistry. 56: 1500-1. PMID 20581082 DOI: 10.1373/clinchem.2010.146175  0.92
2010 Borowitz MJ, Craig FE, Digiuseppe JA, Illingworth AJ, Rosse W, Sutherland DR, Wittwer CT, Richards SJ. Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry. Cytometry. Part B, Clinical Cytometry. 78: 211-30. PMID 20533382 DOI: 10.1002/cyto.b.20525  0.92
2010 Zhou L, Palais RA, Smith GD, Anderson D, Rowe LR, Wittwer CT. Enrichment and detection of rare alleles by means of snapback primers and rapid-cycle PCR. Clinical Chemistry. 56: 814-22. PMID 20299678 DOI: 10.1373/clinchem.2009.142034  0.92
2010 Montgomery JL, Sanford LN, Wittwer CT. High-resolution DNA melting analysis in clinical research and diagnostics. Expert Review of Molecular Diagnostics. 10: 219-40. PMID 20214540 DOI: 10.1586/erm.09.84  0.92
2010 Kumánovics A, Wittwer CT, Pryor RJ, Augustine NH, Leppert MF, Carey JC, Ochs HD, Wedgwood RJ, Faville RJ, Quie PG, Hill HR. Rapid molecular analysis of the STAT3 gene in Job syndrome of hyper-IgE and recurrent infectious diseases. The Journal of Molecular Diagnostics : Jmd. 12: 213-9. PMID 20093388 DOI: 10.2353/jmoldx.2010.090080  0.92
2010 Erali M, Wittwer CT. High resolution melting analysis for gene scanning. Methods (San Diego, Calif.). 50: 250-61. PMID 20085814 DOI: 10.1016/j.ymeth.2010.01.013  0.92
2010 Sundberg SO, Wittwer CT, Gao C, Gale BK. Spinning disk platform for microfluidic digital polymerase chain reaction. Analytical Chemistry. 82: 1546-50. PMID 20085301 DOI: 10.1021/ac902398c  0.92
2010 Hill HR, Wittwer CT. Reply Pediatric Infectious Disease Journal. 29: 191. DOI: 10.1097/01.inf.0000368378.02858.f0  0.92
2010 McKinney JT, Nay LM, De Koeyer D, Reed GH, Wall M, Palais RA, Jarret RL, Wittwer CT. Mutation Scanning and Genotyping in Plants by High-Resolution DNA Melting The Handbook of Plant Mutation Screening: Mining of Natural and Induced Alleles. 149-165. DOI: 10.1002/9783527629398.ch9  0.92
2009 Parant JM, George SA, Pryor R, Wittwer CT, Yost HJ. A rapid and efficient method of genotyping zebrafish mutants. Developmental Dynamics : An Official Publication of the American Association of Anatomists. 238: 3168-74. PMID 19890916 DOI: 10.1002/dvdy.22143  0.92
2009 Margraf RL, Calderon FR, Mao R, Wittwer CT. RET mutation scanning update: exon 15. Clinical Chemistry. 55: 2059-61. PMID 19745057 DOI: 10.1373/clinchem.2009.132209  0.92
2009 Erali M, Pattison DC, Wittwer CT, Petti CA. Human papillomavirus genotyping using an automated film-based chip array. The Journal of Molecular Diagnostics : Jmd. 11: 439-45. PMID 19644025 DOI: 10.2353/jmoldx.2009.080154  0.92
2009 Seipp MT, Durtschi JD, Voelkerding KV, Wittwer CT. Multiplex amplicon genotyping by high-resolution melting. Journal of Biomolecular Techniques : Jbt. 20: 160-4. PMID 19568454  0.92
2009 Bender JM, Rand TH, Ampofo K, Pavia AT, Schober M, Tebo A, Pasi B, Augustine NH, Pryor RJ, Wittwer CT, Hill HR. Family clusters of variant X-linked chronic granulomatous disease. The Pediatric Infectious Disease Journal. 28: 529-33. PMID 19483518 DOI: 10.1097/INF.0b013e318194cf09  0.92
2009 Wittwer CT. High-resolution DNA melting analysis: advancements and limitations. Human Mutation. 30: 857-9. PMID 19479960 DOI: 10.1002/humu.20951  0.92
2009 Lo YM, Wittwer CT. Molecular diagnostics: at the cutting edge of translational research. Clinical Chemistry. 55: 601. PMID 19325016 DOI: 10.1373/clinchem.2008.119586  0.92
2009 Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry. 55: 611-22. PMID 19246619 DOI: 10.1373/clinchem.2008.112797  0.92
2009 Crews N, Wittwer CT, Montgomery J, Pryor R, Gale B. Spatial DNA melting analysis for genotyping and variant scanning. Analytical Chemistry. 81: 2053-8. PMID 19228012 DOI: 10.1021/ac801495w  0.92
2009 Palais R, Wittwer CT. Mathematical algorithms for high-resolution DNA melting analysis. Methods in Enzymology. 454: 323-43. PMID 19216933 DOI: 10.1016/S0076-6879(08)03813-5  0.92
2009 Lyon E, Wittwer CT. LightCycler technology in molecular diagnostics. The Journal of Molecular Diagnostics : Jmd. 11: 93-101. PMID 19196999 DOI: 10.2353/jmoldx.2009.080094  0.92
2009 Margraf RL, Crockett DK, Krautscheid PM, Seamons R, Calderon FR, Wittwer CT, Mao R. Multiple endocrine neoplasia type 2 RET protooncogene database: repository of MEN2-associated RET sequence variation and reference for genotype/phenotype correlations. Human Mutation. 30: 548-56. PMID 19177457 DOI: 10.1002/humu.20928  0.92
2009 Wittwer CT, Rasmussen RP, Ririe KM. Rapid polymerase chain reaction and melting analysis The Pcr Revolution: Basic Technologies and Applications. 48-69. DOI: 10.1017/CBO9780511818974.006  0.92
2009 Farrar JS, Reed GH, Wittwer CT. High-Resolution Melting Curve Analysis for Molecular Diagnostics Molecular Diagnostics: Second Edition. 229-245. DOI: 10.1016/B978-0-12-374537-8.00015-8  0.92
2008 Willmore-Payne C, Holden JA, Wittwer CT, Layfield LJ. The use of EGFR exon 19 and 21 unlabeled DNA probes to screen for activating mutations in non-small cell lung cancer. Journal of Biomolecular Techniques : Jbt. 19: 217-24. PMID 19137110  0.92
2008 Zhou L, Errigo RJ, Lu H, Poritz MA, Seipp MT, Wittwer CT. Snapback primer genotyping with saturating DNA dye and melting analysis. Clinical Chemistry. 54: 1648-56. PMID 18676584 DOI: 10.1373/clinchem.2008.107615  0.92
2008 Erali M, Voelkerding KV, Wittwer CT. High resolution melting applications for clinical laboratory medicine. Experimental and Molecular Pathology. 85: 50-8. PMID 18502416 DOI: 10.1016/j.yexmp.2008.03.012  0.92
2008 Elenitoba-Johnson O, David D, Crews N, Wittwer CT. Plastic versus glass capillaries for rapid-cycle PCR. Biotechniques. 44: 487-8, 490, 492. PMID 18476813 DOI: 10.2144/000112722  0.92
2008 Gundry CN, Dobrowolski SF, Martin YR, Robbins TC, Nay LM, Boyd N, Coyne T, Wall MD, Wittwer CT, Teng DH. Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons. Nucleic Acids Research. 36: 3401-8. PMID 18448472 DOI: 10.1093/nar/gkn204  0.92
2008 Margraf RL, Mao R, Wittwer CT. Rapid diagnosis of MEN2B using unlabeled probe melting analysis and the LightCycler 480 instrument. The Journal of Molecular Diagnostics : Jmd. 10: 123-8. PMID 18258924 DOI: 10.2353/jmoldx.2008.070111  0.92
2008 Seipp MT, Pattison D, Durtschi JD, Jama M, Voelkerding KV, Wittwer CT. Quadruplex genotyping of F5, F2, and MTHFR variants in a single closed tube by high-resolution amplicon melting. Clinical Chemistry. 54: 108-15. PMID 17981920 DOI: 10.1373/clinchem.2007.097121  0.92
2007 Montgomery J, Wittwer CT, Kent JO, Zhou L. Scanning the cystic fibrosis transmembrane conductance regulator gene using high-resolution DNA melting analysis. Clinical Chemistry. 53: 1891-8. PMID 17890437 DOI: 10.1373/clinchem.2007.092361  0.92
2007 Dames S, Pattison DC, Bromley LK, Wittwer CT, Voelkerding KV. Unlabeled probes for the detection and typing of herpes simplex virus. Clinical Chemistry. 53: 1847-54. PMID 17720894 DOI: 10.1373/clinchem.2007.090761  0.92
2007 Poulson MD, Wittwer CT. Closed-tube genotyping of apolipoprotein E by isolated-probe PCR with multiple unlabeled probes and high-resolution DNA melting analysis. Biotechniques. 43: 87-91. PMID 17695257 DOI: 10.2144/000112459  0.92
2007 Dames S, Margraf RL, Pattison DC, Wittwer CT, Voelkerding KV. Characterization of aberrant melting peaks in unlabeled probe assays. The Journal of Molecular Diagnostics : Jmd. 9: 290-6. PMID 17591927 DOI: 10.2353/jmoldx.2007.060139  0.92
2007 Seipp MT, Durtschi JD, Liew MA, Williams J, Damjanovich K, Pont-Kingdon G, Lyon E, Voelkerding KV, Wittwer CT. Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting. The Journal of Molecular Diagnostics : Jmd. 9: 284-9. PMID 17591926 DOI: 10.2353/jmoldx.2007.060136  0.92
2007 Reed GH, Kent JO, Wittwer CT. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics. 8: 597-608. PMID 17559349 DOI: 10.2217/14622416.8.6.597  0.92
2007 Herrmann MG, Durtschi JD, Wittwer CT, Voelkerding KV. Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping. Clinical Chemistry. 53: 1544-8. PMID 17556647 DOI: 10.1373/clinchem.2007.088120  0.92
2007 Vandersteen JG, Bayrak-Toydemir P, Palais RA, Wittwer CT. Identifying common genetic variants by high-resolution melting. Clinical Chemistry. 53: 1191-8. PMID 17525106 DOI: 10.1373/clinchem.2007.085407  0.92
2007 Montgomery J, Wittwer CT, Palais R, Zhou L. Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis. Nature Protocols. 2: 59-66. PMID 17401339 DOI: 10.1038/nprot.2007.10  0.92
2007 Margraf RL, Mao R, Highsmith WE, Holtegaard LM, Wittwer CT. RET proto-oncogene genotyping using unlabeled probes, the masking technique, and amplicon high-resolution melting analysis. The Journal of Molecular Diagnostics : Jmd. 9: 184-96. PMID 17384210 DOI: 10.2353/jmoldx.2007.060091  0.92
2007 Herrmann MG, Durtschi JD, Bromley LK, Wittwer CT, Voelkerding KV. Instrument comparison for heterozygote scanning of single and double heterozygotes: a correction and extension of Herrmann et al., Clin Chem 2006;52:494-503. Clinical Chemistry. 53: 150-2. PMID 17202505 DOI: 10.1373/clinchem.2006.081240  0.92
2007 Sundberg SO, Wittwer CT, Greer J, Pryor RJ, Elenitoba-Johnson O, Gale BK. Solution-phase DNA mutation scanning and SNP genotyping by nanoliter melting analysis. Biomedical Microdevices. 9: 159-66. PMID 17165128 DOI: 10.1007/s10544-006-9017-3  0.92
2007 Greer J, Sundberg SO, Wittwer CT, Gale BK. Comparison of glass etching to xurography prototyping of microfluidic channels for DNA melting analysis Journal of Micromechanics and Microengineering. 17: 2407-2413. DOI: 10.1088/0960-1317/17/12/003  0.92
2007 Sundberg SO, Greer J, Wittwer CT, Gale BK. Miniaturization of warfarin metabolism genotyping by melting analysis using asymmetric PCR and unlabeled oligonucleotide probes Aiche Annual Meeting, Conference Proceedings 0.92
2006 Pryor RJ, Wittwer CT. Real-time polymerase chain reaction and melting curve analysis. Methods in Molecular Biology (Clifton, N.J.). 336: 19-32. PMID 16916250 DOI: 10.1385/1-59745-074-X:19  0.92
2006 Erali M, Pounder JI, Woods GL, Petti CA, Wittwer CT. Multiplex single-color PCR with amplicon melting analysis for identification of Aspergillus species. Clinical Chemistry. 52: 1443-5. PMID 16798978 DOI: 10.1373/clinchem.2006.068510  0.92
2006 Willmore-Payne C, Holden JA, Zhou H, Gupta D, Hirschowitz S, Wittwer CT, Layfield LJ. Evaluation of Her-2/neu gene status in osteosarcoma by fluorescence in situ hybridization and multiplex and monoplex polymerase chain reactions. Archives of Pathology & Laboratory Medicine. 130: 691-8. PMID 16683887 DOI: 10.1043/1543-2165(2006)130[691:EONGSI]2.0.CO;2  0.92
2006 Herrmann MG, Durtschi JD, Bromley LK, Wittwer CT, Voelkerding KV. Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes. Clinical Chemistry. 52: 494-503. PMID 16423901 DOI: 10.1373/clinchem.2005.063438  0.92
2006 Margraf RL, Mao R, Wittwer CT. Masking selected sequence variation by incorporating mismatches into melting analysis probes. Human Mutation. 27: 269-78. PMID 16411177 DOI: 10.1002/humu.20290  0.92
2006 Margraf RL, Mao R, Highsmith WE, Holtegaard LM, Wittwer CT. Mutation scanning of the RET protooncogene using high-resolution melting analysis. Clinical Chemistry. 52: 138-41. PMID 16391329 DOI: 10.1373/clinchem.2005.052951  0.92
2006 Herrmann MG, Durtschi JD, Voelkerding KV, Wittwer CT. Instrument Comparison for DNA Genotyping by Amplicon Melting Jala - Journal of the Association For Laboratory Automation. 11: 273-277. DOI: 10.1007/s11258-005-9072-5  0.92
2006 Sundberg SO, Greer J, Wittwer CT, Pryor RJ, Elenitoba-Johnson O, Gale BK. Homogeneous DNA melting analysis for mutation scanning using nanoliter volumes Micro Total Analysis Systems - Proceedings of Microtas 2006 Conference: 10th International Conference On Miniaturized Systems For Chemistry and Life Sciences. 1441-1443.  0.92
2005 Chou LS, Meadows C, Wittwer CT, Lyon E. Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis. Biotechniques. 39: 644, 646, 648 passim. PMID 16312213 DOI: 10.2144/000112050  0.92
2005 Chou LS, Lyon E, Wittwer CT. A comparison of high-resolution melting analysis with denaturing high-performance liquid chromatography for mutation scanning: cystic fibrosis transmembrane conductance regulator gene as a model. American Journal of Clinical Pathology. 124: 330-8. PMID 16191501 DOI: 10.1309/BF3M-LJN8-J527-MWQY  0.92
2005 Zhou L, Wang L, Palais R, Pryor R, Wittwer CT. High-resolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution. Clinical Chemistry. 51: 1770-7. PMID 16189378 DOI: 10.1373/clinchem.2005.054924  0.92
2005 Palais RA, Liew MA, Wittwer CT. Quantitative heteroduplex analysis for single nucleotide polymorphism genotyping. Analytical Biochemistry. 346: 167-75. PMID 16188219 DOI: 10.1016/j.ab.2005.08.010  0.92
2005 Lewis TB, Robison JE, Bastien R, Milash B, Boucher K, Samlowski WE, Leachman SA, Dirk Noyes R, Wittwer CT, Perreard L, Bernard PS. Molecular classification of melanoma using real-time quantitative reverse transcriptase-polymerase chain reaction. Cancer. 104: 1678-86. PMID 16116595 DOI: 10.1002/cncr.21372  0.92
2005 Graham R, Liew M, Meadows C, Lyon E, Wittwer CT. Distinguishing different DNA heterozygotes by high-resolution melting. Clinical Chemistry. 51: 1295-8. PMID 15905310 DOI: 10.1373/clinchem.2005.051516  0.92
2005 Odell ID, Cloud JL, Seipp M, Wittwer CT. Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting analysis of hsp65 PCR products. American Journal of Clinical Pathology. 123: 96-101. PMID 15762284 DOI: 10.1309/WDR0-82X9-FFJB-QQGB  0.92
2005 Gingeras TR, Higuchi R, Kricka LJ, Lo YM, Wittwer CT. Fifty years of molecular (DNA/RNA) diagnostics. Clinical Chemistry. 51: 661-71. PMID 15650028 DOI: 10.1373/clinchem.2004.045336  0.92
2004 Margraf RL, Erali M, Liew M, Wittwer CT. Genotyping hepatitis C virus by heteroduplex mobility analysis using temperature gradient capillary electrophoresis. Journal of Clinical Microbiology. 42: 4545-51. PMID 15472307 DOI: 10.1128/JCM.42.10.4545-4551.2004  0.92
2004 Willmore C, Holden JA, Zhou L, Tripp S, Wittwer CT, Layfield LJ. Detection of c-kit-activating mutations in gastrointestinal stromal tumors by high-resolution amplicon melting analysis. American Journal of Clinical Pathology. 122: 206-16. PMID 15323137 DOI: 10.1309/4E6U-YBY6-2N2F-CA6N  0.92
2004 Margraf RL, Page S, Erali M, Wittwer CT. Single-tube method for nucleic acid extraction, amplification, purification, and sequencing. Clinical Chemistry. 50: 1755-61. PMID 15308595 DOI: 10.1373/clinchem.2004.035808  0.92
2004 Reed GH, Wittwer CT. Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clinical Chemistry. 50: 1748-54. PMID 15308590 DOI: 10.1373/clinchem.2003.029751  0.92
2004 Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais B, Wittwer CT. High-resolution DNA melting curve analysis to establish HLA genotypic identity Tissue Antigens. 64: 156-164. PMID 15245370 DOI: 10.1111/j.1399-0039.2004.00248.x  0.92
2004 Zhou L, Myers AN, Vandersteen JG, Wang L, Wittwer CT. Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye. Clinical Chemistry. 50: 1328-35. PMID 15166111 DOI: 10.1373/clinchem.2004.034322  0.92
2003 García-Villalba P, Denkers ND, Wittwer CT, Hoff C, Nelson RD, Mauch TJ. Real-time PCR quantification of AT1 and AT2 angiotensin receptor mRNA expression in the developing rat kidney. Nephron. Experimental Nephrology. 94: e154-9. PMID 12972714  0.92
2003 Millson A, Suli A, Hartung L, Kunitake S, Bennett A, Nordberg MC, Hanna W, Wittwer CT, Seth A, Lyon E. Comparison of two quantitative polymerase chain reaction methods for detecting HER2/neu amplification. The Journal of Molecular Diagnostics : Jmd. 5: 184-90. PMID 12876209 DOI: 10.1016/S1525-1578(10)60471-0  0.92
2003 Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clinical Chemistry. 49: 853-60. PMID 12765979 DOI: 10.1373/49.6.853  0.92
2003 Gundry CN, Vandersteen JG, Reed GH, Pryor RJ, Chen J, Wittwer CT. Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes. Clinical Chemistry. 49: 396-406. PMID 12600951 DOI: 10.1373/49.3.396  0.92
2002 Millward H, Samowitz W, Wittwer CT, Bernard PS. Homogeneous amplification and mutation scanning of the p53 gene using fluorescent melting curves. Clinical Chemistry. 48: 1321-8. PMID 12142390  0.92
2002 Bernard PS, Wittwer CT. Real-time PCR technology for cancer diagnostics. Clinical Chemistry. 48: 1178-85. PMID 12142370  0.92
2001 Wittwer CT, Herrmann MG, Gundry CN, Elenitoba-Johnson KSJ. Real-time multiplex PCR assays Methods. 25: 430-442. PMID 11846612 DOI: 10.1006/meth.2001.1265  0.92
2001 Von Ahsen N, Wittwer CT, Schütz E. Oligonucleotide melting temperatures under PCR conditions: Nearest-neighbor corrections for MG2+, deoxynucleotide triphosphate, and dimethyl sulfoxide concentrations with comparison to alternative empirical formulas Clinical Chemistry. 47: 1956-1961. PMID 11673362  0.92
2001 Lyon E, Millson A, Lowery MC, Woods R, Wittwer CT. Quantification of HER2/neu gene amplification by competitive PCR using fluorescent melting curve analysis Clinical Chemistry. 47: 844-851. PMID 11325887  0.92
2001 Crockett AO, Wittwer CT. Fluorescein-labeled oligonucleotides for real-time PCR: Using the inherent quenching of deoxyguanosine nucleotides Analytical Biochemistry. 290: 89-97. PMID 11180941 DOI: 10.1006/abio.2000.4957  0.92
2001 Elenitoba-Johnson KSJ, Bohling SD, Wittwer CT, King TC. Multiplex PCR by multicolor fluorimetry and fluorescence melting curve analysis Nature Medicine. 7: 249-253. PMID 11175859 DOI: 10.1038/84708  0.92
2000 Paddock S, McDougal D, Heap DM, Herrmann MG, Wittwer CT. Making movies on a weekend Biotechniques. 29: 997. PMID 11084861  0.92
2000 Millson AS, Spangler FL, Wittwer CT, Lyon E. Comparison of automated short tandem repeat and manual variable number of tandem repeat analysis of chimerism in bone marrow transplant patients Diagnostic Molecular Pathology. 9: 91-97. PMID 10850545 DOI: 10.1097/00019606-200006000-00005  0.92
2000 De Silva D, Wittwer CT. Monitoring hybridization during polymerase chain reaction Journal of Chromatography B: Biomedical Sciences and Applications. 741: 3-13. PMID 10839126 DOI: 10.1016/S0378-4347(00)00055-4  0.92
2000 Herrmann MG, Dobrowolski SF, Wittwer CT. Rapid β-globin genotyping by multiplexing probe melting temperature and color Clinical Chemistry. 46: 425-428. PMID 10702535  0.92
2000 Bernard PS, Wittwer CT. Homogeneous amplification and variant detection by fluorescent hybridization probes Clinical Chemistry. 46: 147-148. PMID 10657368  0.92
1999 Gundry CN, Bernard PS, Herrmann MG, Reed GH, Wittwer CT. Rapid F508del and F508C assay using fluorescent hybridization probes Genetic Testing. 3: 365-370. PMID 10627945  0.92
1999 Bernard PS, Pritham GH, Wittwer CT. Color multiplexing hybridization probes using the apolipoprotein E locus as a model system for genotyping Analytical Biochemistry. 273: 221-228. PMID 10469493 DOI: 10.1006/abio.1999.4217  0.92
1999 Hussey CE, Lyon E, Millson A, Lay MJ, Wittwer CT, Segal GH. A rapid practical RT-PCR-based approach for the detection of the PML/RARalpha fusion transcript in acute promyelocytic leukemia American Journal of Clinical Pathology. 112: 256-262. PMID 10439807  0.92
1999 Bohling SD, Wittwer CT, King TC, Elenitoba-Johnson KSJ. Fluorescence melting curve analysis for the detection of the bcl-1/JH translocation in mantle cell lymphoma Laboratory Investigation. 79: 337-345. PMID 10092070  0.92
1999 Bohling SD, King TC, Wittwer CT, Elenitoba-Johnson KSJ. Rapid simultaneous amplification and detection of the MBR/JH chromosomal translocation by fluorescence melting curve analysis American Journal of Pathology. 154: 97-103. PMID 9916923  0.92
1998 Bernard PS, Ajioka RS, Kushner JP, Wittwer CT. Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes American Journal of Pathology. 153: 1055-1061. PMID 9777937  0.92
1998 Morrison TB, Weis JJ, Wittwer CT. Quantification of lowcopy transcripts by continuous SYBR® green I monitoring during amplification Biotechniques. 24: 954-962. PMID 9631186  0.92
1998 Bernard PS, Lay MJ, Wittwer CT. Integrated amplification and detection of the C677T point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves Analytical Biochemistry. 255: 101-107. PMID 9448847 DOI: 10.1006/abio.1997.2427  0.92
1998 Lyon E, Millson A, Phan T, Wittwer CT. Detection and identification of base alterations within the region of factor V leiden by fluorescent melting curves Molecular Diagnosis. 3: 203-210. DOI: 10.1016/S1084-8592(98)80041-6  0.92
1998 Pritham GH, Wittwer CT. Continuous fluorescent monitoring of rapid cycle polymerase chain reaction Journal of Clinical Ligand Assay. 21: 404-412.  0.92
1997 Lay MJ, Wittwer CT. Real-time fluorescence genotyping of factor V leiden during rapid-cycle PCR Clinical Chemistry. 43: 2262-2267. PMID 9439442  0.92
1997 Mouritsen CL, Wittwer CT, Reed G, Khan TM, Martins TB, Jaskowski TD, Litwin CM, Hill HR. Detection of Epstein-Barr viral DNA in serum using rapid-cycle PCR Biochemical and Molecular Medicine. 60: 161-168. PMID 9169098 DOI: 10.1006/bmme.1997.2571  0.92
1997 Swerdlow H, Jones BJ, Wittwer CT. Fully automated DNA reaction and analysis in a fluidic capillary instrument. Analytical Chemistry. 69: 848-55. PMID 9068272  0.92
1997 Ririe KM, Rasmussen RP, Wittwer CT. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction Analytical Biochemistry. 245: 154-160. PMID 9056205 DOI: 10.1006/abio.1996.9916  0.92
1997 Wittwer CT, Ririe KM, Andrew RV, David DA, Gundry RA, Balis UJ. The LightCycler(TM): A microvolume multisample fluorimeter with rapid temperature control Biotechniques. 22: 176-181. PMID 8994665  0.92
1996 Segal GH, Hussey CE, Wittwer CT. PCR for T-cell rearrangements Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B. 5: 297-298. PMID 8955623  0.92
1996 Florell SR, Townsend JJ, Klatt EC, Pysher TJ, Coffin CM, Wittwer CT, Viskochil DH. Aprosencephaly and cerebellar dysgenesis in sibs. American Journal of Medical Genetics. 63: 542-8. PMID 8826432 DOI: 10.1002/(SICI)1096-8628(19960628)63:4<542::AID-AJMG6>3.0.CO;2-Q  0.92
1996 Endo M, Beatty PG, Vreeke TM, Wittwer CT, Singh SP, Parker CJ. Syngeneic bone marrow transplantation without conditioning in a patient with paroxysmal nocturnal hemoglobinuria: In vivo evidence that the mutant stem cells have a survival advantage Blood. 88: 742-750. PMID 8695823  0.92
1996 Mouritsen CL, Wittwer CT, Litwin CM, Yang L, Weis JJ, Martins TB, Jaskowski TD, Hill HR. Polymerase chain reaction detection of Lyme disease: Correlation with clinical manifestations and serologic responses American Journal of Clinical Pathology. 105: 647-654. PMID 8623775  0.92
1995 Lim LC, Segal GH, Wittwer CT. Detection of bcl-1 gene rearrangement and B-cell clonality in mantle cell lymphoma using formalin-fixed, paraffin-embedded tissues American Journal of Clinical Pathology. 104: 689-695. PMID 8526214  0.92
1994 Molot RJ, Meeker TC, Wittwer CT, Perkins SL, Segal GH, Masih AS, Braylan RC, Kjeldsberg CR. Antigen expression and polymerase chain reaction amplification of mantle cell lymphomas Blood. 83: 1626-1631. PMID 8123854  0.92
1993 Albro J, Bauer KD, Hitchcock CL, Wittwer CT. Improved DNA content histograms from formalin-fixed, paraffin-embedded liver tissue by proteinase K digestion Cytometry. 14: 673-678. PMID 8404374  0.92
1993 Wittwer CT, Marshall BC, Reed GH, Cherry JL. Rapid cycle allele-specific amplification: Studies with the cystic fibrosis ΔF508 locus Clinical Chemistry. 39: 804-809. PMID 7683581  0.92
1992 Weis JH, Tan SS, Martin BK, Wittwer CT. Detection of rare mRNAs via quantitative RT-PCR Trends in Genetics. 8: 263-264. PMID 1509514 DOI: 10.1016/0168-9525(92)90242-V  0.92
1992 Segal GH, Wittwer CT, Fishleder AJ, Stoler MH, Tubbs RR, Kjeldsberg CR. Identification of monoclonal B-cell populations by rapid cycle polymerase chain reaction: A practical screening method for the detection of immunoglobulin gene rearrangements American Journal of Pathology. 141: 1291-1297. PMID 1466395  0.92
1992 Kershisnik MM, Knisely AS, Sun CC, Andrews JM, Wittwer CT. Cytomegalovirus infection, fetal liver disease, and neonatal hemochromatosis. Human Pathology. 23: 1075-80. PMID 1325409 DOI: 10.1016/0046-8177(92)90272-5  0.92
1992 Holden JA, Rolfson DH, Wittwer CT. The distribution of immunoreactive topoisomerase II protein in human tissues and neoplasms Oncology Research. 4: 157-166. PMID 1324031  0.92
1992 Hopfenbeck JA, Holden JA, Wittwer CT, Kjeldsberg CR. Digoxigenin-labeled probes amplified from genomic DNA detect T-cell gene rearrangements American Journal of Clinical Pathology. 97: 638-644. PMID 1315485  0.92
1991 Wittwer CT, Garling DJ. Rapid cycle DNA amplification: Time and temperature optimization Biotechniques. 10. PMID 2003928  0.92
1991 O'Connell JB, Renlund DG, Hammond EH, Wittwer CT, Yowell RL, DeWitt CW, Jones KW, Gay WA, Menlove RL, Bristow MR. Sensitization to OKT3 monoclonal antibody in heart transplantation: Correlation with early allograft loss Journal of Heart and Lung Transplantation. 10: 217-222. PMID 1903303  0.92
1990 Wittwer CT, Beck S, Peterson M, Davidson R, Wilson DE, Hansen RG. Mild pantothenate deficiency in rats elevates serum triglyceride and free fatty acid levels. The Journal of Nutrition. 120: 719-25. PMID 2366105  0.92
1990 Rabkin MS, Wittwer CT, Soong VY. Flow cytometric DNA content analysis of a case of pilomatrix carcinoma showing multiple recurrences and invasion of the cranial vault Journal of the American Academy of Dermatology. 23: 104-108. PMID 2365860  0.92
1990 Wittwer CT, Fillmore GC, Garling DJ. Minimizing the time required for DNA amplification by efficient heat transfer to small samples Analytical Biochemistry. 186: 328-331. PMID 2363506 DOI: 10.1016/0003-2697(90)90090-V  0.92
1990 Holden JA, Rolfson DH, Wittwer CT. Human DNA topoisomerase II: Evaluation of enzyme activity in normal and neoplastic tissues Biochemistry®. 2127-2134. PMID 2158345  0.92
1990 Hammond EH, Wittwer CT, Greenwood J, Knape WA, Yowell RL, Menlove RL, Craven C, Renlund DG, Bristow MR, Dewitt CW, O’connell JB. Relationship of OKT3 sensitization and vascular rejection in cardiac transplant patients receiving OKT3 rejection prophylaxis Transplantation. 50: 776-782. PMID 2122559  0.92
1990 Rabkin MS, Kjeldsberg CR, Wittwer CT, Marty J. A comparison study of two methods of peanut agglutinin staining with S100 immunostaining in 29 cases of histiocytosis X (Langerhans' cell histiocytosis) Archives of Pathology and Laboratory Medicine. 114: 511-515. PMID 1692202  0.92
1989 Wittwer CT, Schweitzer C, Pearson J, Song WO, Windham CT, Wyse BW, Hansen RG. Enzymes for liberation of pantothenic acid in blood: use of plasma pantetheinase. The American Journal of Clinical Nutrition. 50: 1072-8. PMID 2816792  0.92
1989 Wittwer CT, Fillmore GC, Hillyard DR. Automated polymerase chain reaction in capillary tubes with hot air Nucleic Acids Research. 17: 4353-4357. PMID 2740218 DOI: 10.1093/nar/17.11.4353  0.92
1989 Wittwer CT, Knape WA, Bristow MR, Gilbert EM, Renlund DG, O’Connell JB, Dewitt CW. The quantitative flow cytometric plasma OKT3 assay: Its potential application in cardiac transplantation Transplantation. 48: 533-535. PMID 2528852  0.92
1988 Wittwer CT, Bristow MR, Michael Gilbert E, Renlund DG, O’Connell JB, Dewitt CW. OKT3 therapy as a cause of high panel-reactive antibodies in serum using standard microcytotoxicity techniques Transplantation. 45: 832-834. PMID 3282368  0.92
1988 Rabkin MS, Kjeldsberg CR, Hammond ME, Wittwer CT, Nathwani B. Clinical, ultrastructural immunohistochemical and DNA content analysis of lymphomas having features of interdigitating reticulum cells Cancer. 61: 1594-1601. PMID 3280115  0.92
1988 Rabkin MS, Wittwer CT, Kjeldsberg CR, Piepkorn MW. Flow-cytometric DNA content of histiocytosis X (Langerhans cell histiocytosis) American Journal of Pathology. 131: 283-289. PMID 3258734  0.92
1988 Pearson SD, Wittwer CT, Owen Ash K. Evaluation of three commercial kits for the confirmation of antibodies to human immunodeficiency virus (HIV-1) Clinical Chemistry. 34: 1930. PMID 3166415  0.92
1987 Wittwer CT, Graves CP, Peterson MA, Jorgensen E, Wilson DE, Thoene JG, Wyse BW, Windham CT, Hansen RG. Pantethine lipomodulation: evidence for cysteamine mediation in vitro and in vivo. Atherosclerosis. 68: 41-9. PMID 3689482 DOI: 10.1016/0021-9150(87)90092-X  0.92
1987 Wittwer CT, Smith AM, Ash KO, Dewitt CW. False-positive antibody tests for human immunodeficiency virus in transplant patients with antilymphocyte antibodies Transplantation. 44: 843-844. PMID 3321595  0.92
1987 Gleeson JM, Wittwer CT, Schipke CA, Wilson DE. Effect of carnitine and pantethine on the metabolic abnormalities of acquired total lipodystrophy Current Therapeutic Research - Clinical and Experimental. 41: 83-88.  0.92
1986 Wittwer CT, Wyse BW, Hansen RG. Enzymatic hydrolysis of pantetheine. Methods in Enzymology. 122: 36-43. PMID 3517567  0.92
1985 Wittwer CT, Gahl WA, Butler JD, Zatz M, Thoene JG. Metabolism of pantethine in cystinosis. The Journal of Clinical Investigation. 76: 1665-72. PMID 4056044 DOI: 10.1172/JCI112152  0.92
1983 Wittwer CT, Burkhard D, Ririe K, Rasmussen R, Brown J, Wyse BW, Hansen RG. Purification and properties of a pantetheine-hydrolyzing enzyme from pig kidney. The Journal of Biological Chemistry. 258: 9733-8. PMID 6885768  0.92
1982 Wittwer C, Wyse B, Hansen RG. Assay of the enzymatic hydrolysis of pantetheine. Analytical Biochemistry. 122: 213-22. PMID 7114442 DOI: 10.1016/0003-2697(82)90273-1  0.92
1979 Wyse BW, Wittwer C, Hansen RG. Radioimmunoassay for pantothenic acid in blood and other tissues. Clinical Chemistry. 25: 108-10. PMID 761345  0.92
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