| Year |
Citation |
Score |
| 2024 |
Fischer K, Lulla A, So TY, Pereyra-Gerber P, Raybould MIJ, Kohler TN, Yam-Puc JC, Kaminski TS, Hughes R, Pyeatt GL, Leiss-Maier F, Brear P, Matheson NJ, Deane CM, Hyvönen M, ... ... Hollfelder F, et al. Rapid discovery of monoclonal antibodies by microfluidics-enabled FACS of single pathogen-specific antibody-secreting cells. Nature Biotechnology. PMID 39143416 DOI: 10.1038/s41587-024-02346-5 |
0.562 |
|
| 2023 |
Ladeveze S, Zurek PJ, Kaminski TS, Emond S, Hollfelder F. Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets. Acs Catalysis. 13: 10232-10243. PMID 37560191 DOI: 10.1021/acscatal.3c01609 |
0.305 |
|
| 2023 |
Yam-Puc JC, Hosseini Z, Horner EC, Gerber PP, Beristain-Covarrubias N, Hughes R, Lulla A, Rust M, Boston R, Ali M, Fischer K, Simmons-Rosello E, O'Reilly M, Robson H, Booth LH, ... ... Hollfelder F, et al. Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade. Nature Communications. 14: 3292. PMID 37369658 DOI: 10.1038/s41467-023-38810-0 |
0.548 |
|
| 2023 |
Knyphausen P, Rangel Pereira M, Brear P, Hyvönen M, Jermutus L, Hollfelder F. Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature Communications. 14: 768. PMID 36765057 DOI: 10.1038/s41467-023-36099-7 |
0.621 |
|
| 2022 |
Neun S, Brear P, Campbell E, Tryfona T, El Omari K, Wagner A, Dupree P, Hyvönen M, Hollfelder F. Functional metagenomic screening identifies an unexpected β-glucuronidase. Nature Chemical Biology. PMID 35799064 DOI: 10.1038/s41589-022-01071-x |
0.62 |
|
| 2022 |
Scheele RA, Lindenburg LH, Petek M, Schober M, Dalby KN, Hollfelder F. Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates. Nature Communications. 13: 844. PMID 35149678 DOI: 10.1038/s41467-022-28396-4 |
0.658 |
|
| 2021 |
Lindenburg LH, Pantelejevs T, Gielen F, Zuazua-Villar P, Butz M, Rees E, Kaminski CF, Downs JA, Hyvönen M, Hollfelder F. Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats. Proceedings of the National Academy of Sciences of the United States of America. 118. PMID 34772801 DOI: 10.1073/pnas.2017708118 |
0.592 |
|
| 2021 |
Holstein JM, Gylstorff C, Hollfelder F. Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput. Acs Synthetic Biology. PMID 33502841 DOI: 10.1021/acssynbio.0c00538 |
0.326 |
|
| 2021 |
Lindenburg L, Hollfelder F. 'NAD-display': Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry. Angewandte Chemie (International Ed. in English). PMID 33470025 DOI: 10.1002/anie.202013486 |
0.323 |
|
| 2020 |
Zurek PJ, Hours R, Schell U, Pushpanath A, Hollfelder F. Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation. Lab On a Chip. PMID 33242058 DOI: 10.1039/d0lc00830c |
0.314 |
|
| 2020 |
Neun S, Zurek PJ, Kaminski TS, Hollfelder F. Ultrahigh throughput screening for enzyme function in droplets. Methods in Enzymology. 643: 317-343. PMID 32896286 DOI: 10.1016/Bs.Mie.2020.06.002 |
0.381 |
|
| 2020 |
Emond S, Petek M, Kay EJ, Heames B, Devenish SRA, Tokuriki N, Hollfelder F. Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis. Nature Communications. 11: 3469. PMID 32651386 DOI: 10.1038/S41467-020-17061-3 |
0.359 |
|
| 2020 |
Kuo ST, Jahn RL, Cheng YJ, Chen YL, Lee YJ, Hollfelder F, Wen JD, Chou HD. Global fitness landscapes of the Shine-Dalgarno sequence. Genome Research. PMID 32424071 DOI: 10.1101/Gr.260182.119 |
0.31 |
|
| 2020 |
Lindenburg L, Huovinen T, van de Wiel K, Herger M, Snaith MR, Hollfelder F. Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins. Nucleic Acids Research. PMID 32383757 DOI: 10.1093/Nar/Gkaa270 |
0.392 |
|
| 2019 |
van Loo B, Heberlein M, Mair P, Zinchenko A, Schüürmann J, Eenink BDG, Holstein JM, Dilkaute C, Jose J, Hollfelder F, Bornberg-Bauer E. High-Throughput, Lysis-free Screening for Sulfatase Activity Using Autodisplay in Microdroplets. Acs Synthetic Biology. PMID 31738524 DOI: 10.1021/Acssynbio.9B00274 |
0.396 |
|
| 2019 |
Neun S, Kaminski TS, Hollfelder F. Single-cell activity screening in microfluidic droplets. Methods in Enzymology. 628: 95-112. PMID 31668237 DOI: 10.1016/Bs.Mie.2019.07.009 |
0.327 |
|
| 2019 |
Buryska T, Vasina M, Gielen F, Vanacek P, van Vliet L, Jezek J, Pilat Z, Zemanek P, Damborský J, Hollfelder F, Prokop Z. Controlled oil/water partitioning of hydrophobic substrates extends the bioanalytical applications of droplet-based microfluidics. Analytical Chemistry. PMID 31240908 DOI: 10.1021/Acs.Analchem.9B01839 |
0.412 |
|
| 2019 |
van Loo B, Berry R, Boonyuen U, Mohamed MF, Golicnik M, Hengge AC, Hollfelder F. Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase. Biochemistry. PMID 30810299 DOI: 10.1021/Acs.Biochem.8B00996 |
0.375 |
|
| 2018 |
van Loo B, Bayer CD, Fischer G, Jonas S, Valkov E, Mohamed MF, Vorobieva A, Dutruel C, Hyvonen M, Hollfelder F. Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily. Journal of the American Chemical Society. PMID 30497259 DOI: 10.1021/Jacs.8B10290 |
0.803 |
|
| 2018 |
Miton CM, Jonas S, Fischer G, Duarte F, Mohamed MF, van Loo B, Kintses B, Kamerlin SCL, Tokuriki N, Hyvönen M, Hollfelder F. Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset. Proceedings of the National Academy of Sciences of the United States of America. PMID 30012610 DOI: 10.1073/Pnas.1607817115 |
0.784 |
|
| 2018 |
van Loo B, Schober M, Valkov E, Heberlein M, Bornberg-Bauer E, Faber K, Hyvönen M, Hollfelder F. Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester. Journal of Molecular Biology. PMID 29458126 DOI: 10.1016/J.Jmb.2018.02.010 |
0.641 |
|
| 2018 |
Gielen F, Colin PY, Mair P, Hollfelder F. Ultrahigh-Throughput Screening of Single-Cell Lysates for Directed Evolution and Functional Metagenomics. Methods in Molecular Biology (Clifton, N.J.). 1685: 297-309. PMID 29086317 DOI: 10.1007/978-1-4939-7366-8_18 |
0.348 |
|
| 2017 |
Heitz M, Kwok A, Eggimann GA, Hollfelder F, Darbre T, Reymond JL. Peptide Dendrimer-Lipid Conjugates as DNA and siRNA Transfection Reagents: Role of Charge Distribution Across Generations. Chimia. 71: 220-225. PMID 28446340 DOI: 10.2533/Chimia.2017.220 |
0.327 |
|
| 2017 |
Mair P, Gielen F, Hollfelder F. Exploring sequence space in search of functional enzymes using microfluidic droplets. Current Opinion in Chemical Biology. 37: 137-144. PMID 28390246 DOI: 10.1016/J.Cbpa.2017.02.018 |
0.411 |
|
| 2017 |
Gielen F, Butz M, Rees EJ, Erdelyi M, Moschetti T, Hyvönen M, Edel JB, Kaminski CF, Hollfelder F. Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets. Analytical Chemistry. 89: 1092-1101. PMID 28192993 DOI: 10.1021/Acs.Analchem.6B02528 |
0.642 |
|
| 2016 |
Kwok A, Eggimann GA, Heitz M, Reymond JL, Hollfelder F, Darbre T. Efficient Transfection of siRNA by Peptide Dendrimer-Lipid Conjugates. Chembiochem : a European Journal of Chemical Biology. PMID 27862758 DOI: 10.1002/Cbic.201600485 |
0.301 |
|
| 2016 |
Gielen F, Hours R, Emond S, Fischlechner M, Schell U, Hollfelder F. Ultrahigh-throughput-directed enzyme evolution by absorbance-activated droplet sorting (AADS). Proceedings of the National Academy of Sciences of the United States of America. 113: E7383-E7389. PMID 27821774 DOI: 10.1073/Pnas.1606927113 |
0.418 |
|
| 2016 |
Mankowska SA, Gatti-Lafranconi P, Chodorge M, Sridharan S, Minter RR, Hollfelder F. A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis. Scientific Reports. 6: 36391. PMID 27819305 DOI: 10.1038/Srep36391 |
0.357 |
|
| 2016 |
Kaltenbach M, Emond S, Hollfelder F, Tokuriki N. Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity. Plos Genetics. 12: e1006305. PMID 27716796 DOI: 10.1371/Journal.Pgen.1006305 |
0.336 |
|
| 2016 |
Campbell E, Kaltenbach M, Correy GJ, Carr PD, Porebski BT, Livingstone EK, Afriat-Jurnou L, Buckle AM, Weik M, Hollfelder F, Tokuriki N, Jackson CJ. The role of protein dynamics in the evolution of new enzyme function. Nature Chemical Biology. PMID 27618189 DOI: 10.1038/Nchembio.2175 |
0.36 |
|
| 2015 |
Colin PY, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvönen M, Morgavi DP, Janssen DB, Hollfelder F. Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nature Communications. 6: 10008. PMID 26639611 DOI: 10.1038/Ncomms10008 |
0.785 |
|
| 2015 |
Fradet E, Bayer C, Hollfelder F, Baroud CN. Measuring Fast and Slow Enzyme Kinetics in Stationary Droplets. Analytical Chemistry. 87: 11915-22. PMID 26524082 DOI: 10.1021/Acs.Analchem.5B03567 |
0.346 |
|
| 2015 |
van Vliet LD, Colin PY, Hollfelder F. Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins. Interface Focus. 5: 20150035. PMID 26464791 DOI: 10.1098/Rsfs.2015.0035 |
0.357 |
|
| 2015 |
Colin PY, Zinchenko A, Hollfelder F. Enzyme engineering in biomimetic compartments. Current Opinion in Structural Biology. 33: 42-51. PMID 26311177 DOI: 10.1016/J.Sbi.2015.06.001 |
0.357 |
|
| 2015 |
Kaltenbach M, Jackson CJ, Campbell EC, Hollfelder F, Tokuriki N. Reverse evolution leads to genotypic incompatibility despite functional and active-site convergence. Elife. 4. PMID 26274563 DOI: 10.7554/Elife.06492 |
0.385 |
|
| 2015 |
Houlihan G, Gatti-Lafranconi P, Lowe D, Hollfelder F. Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process. Protein Engineering, Design & Selection : Peds. 28: 269-79. PMID 26134501 DOI: 10.1093/Protein/Gzv029 |
0.349 |
|
| 2015 |
Shafee T, Gatti-Lafranconi P, Minter R, Hollfelder F. Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease. Chembiochem : a European Journal of Chemical Biology. PMID 26097079 DOI: 10.1002/Cbic.201500295 |
0.341 |
|
| 2015 |
Chen W, Avezov E, Schlachter SC, Gielen F, Laine RF, Harding HP, Hollfelder F, Ron D, Kaminski CF. A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth. Biophysical Journal. 108: 999-1002. PMID 25762312 DOI: 10.1016/J.Bpj.2015.01.012 |
0.305 |
|
| 2015 |
Gielen F, Buryska T, Van Vliet L, Butz M, Damborsky J, Prokop Z, Hollfelder F. Interfacing microwells with nanoliter compartments: a sampler generating high-resolution concentration gradients for quantitative biochemical analyses in droplets. Analytical Chemistry. 87: 624-32. PMID 25496166 DOI: 10.1021/Ac503336G |
0.352 |
|
| 2014 |
Fischlechner M, Schaerli Y, Mohamed MF, Patil S, Abell C, Hollfelder F. Evolution of enzyme catalysts caged in biomimetic gel-shell beads. Nature Chemistry. 6: 791-6. PMID 25143214 DOI: 10.1038/Nchem.1996 |
0.747 |
|
| 2014 |
Villiers B, Hollfelder F. USER friendly DNA recombination (USERec): gene library construction requiring minimal sequence homology. Methods in Molecular Biology (Clifton, N.J.). 1179: 213-24. PMID 25055781 DOI: 10.1007/978-1-4939-1053-3_15 |
0.328 |
|
| 2014 |
Zinchenko A, Devenish SR, Kintses B, Colin PY, Fischlechner M, Hollfelder F. One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution. Analytical Chemistry. 86: 2526-33. PMID 24517505 DOI: 10.1021/Ac403585P |
0.741 |
|
| 2014 |
Houlihan G, Gatti-Lafranconi P, Kaltenbach M, Lowe D, Hollfelder F. An experimental framework for improved selection of binding proteins using SNAP display. Journal of Immunological Methods. 405: 47-56. PMID 24456628 DOI: 10.1016/J.Jim.2014.01.006 |
0.37 |
|
| 2013 |
Schober M, Toesch M, Knaus T, Strohmeier GA, van Loo B, Fuchs M, Hollfelder F, Macheroux P, Faber K. One-Pot Deracemization of sec-Alcohols: Enantioconvergent Enzymatic Hydrolysis of Alkyl Sulfates Using Stereocomplementary Sulfatases. Angewandte Chemie (Weinheim An Der Bergstrasse, Germany). 125: 3359-3361. PMID 25821253 DOI: 10.1002/Ange.201209946 |
0.355 |
|
| 2013 |
Diamante L, Gatti-Lafranconi P, Schaerli Y, Hollfelder F. In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Protein Engineering, Design & Selection : Peds. 26: 713-24. PMID 23980186 DOI: 10.1093/Protein/Gzt039 |
0.724 |
|
| 2013 |
Gatti-Lafranconi P, Dijkman WP, Devenish SR, Hollfelder F. A single mutation in the core domain of the lac repressor reduces leakiness. Microbial Cell Factories. 12: 67. PMID 23834731 DOI: 10.1186/1475-2859-12-67 |
0.342 |
|
| 2013 |
Shim JU, Ranasinghe RT, Smith CA, Ibrahim SM, Hollfelder F, Huck WT, Klenerman D, Abell C. Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays. Acs Nano. 7: 5955-64. PMID 23805985 DOI: 10.1021/Nn401661D |
0.353 |
|
| 2013 |
Kwok A, Eggimann GA, Reymond JL, Darbre T, Hollfelder F. Peptide dendrimer/lipid hybrid systems are efficient DNA transfection reagents: structure--activity relationships highlight the role of charge distribution across dendrimer generations. Acs Nano. 7: 4668-82. PMID 23682947 DOI: 10.1021/Nn400343Z |
0.345 |
|
| 2013 |
Gielen F, van Vliet L, Koprowski BT, Devenish SR, Fischlechner M, Edel JB, Niu X, deMello AJ, Hollfelder F. A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition. Analytical Chemistry. 85: 4761-9. PMID 23614771 DOI: 10.1021/Ac400480Z |
0.376 |
|
| 2013 |
Devenish SR, Kaltenbach M, Fischlechner M, Hollfelder F. Droplets as reaction compartments for protein nanotechnology. Methods in Molecular Biology (Clifton, N.J.). 996: 269-86. PMID 23504430 DOI: 10.1007/978-1-62703-354-1_16 |
0.333 |
|
| 2013 |
Houlihan G, Lowe D, Hollfelder F. SNAP display - an in vitro method for the selection of protein binders. Current Pharmaceutical Design. 19: 5421-8. PMID 23431984 DOI: 10.2174/1381612811319300012 |
0.326 |
|
| 2013 |
Gatti-Lafranconi P, Hollfelder F. Flexibility and reactivity in promiscuous enzymes. Chembiochem : a European Journal of Chemical Biology. 14: 285-92. PMID 23362046 DOI: 10.1002/Cbic.201200628 |
0.38 |
|
| 2013 |
Mohamed MF, Hollfelder F. Efficient, crosswise catalytic promiscuity among enzymes that catalyze phosphoryl transfer. Biochimica Et Biophysica Acta. 1834: 417-24. PMID 22885024 DOI: 10.1016/J.Bbapap.2012.07.015 |
0.393 |
|
| 2012 |
Kintses B, Hein C, Mohamed MF, Fischlechner M, Courtois F, Lainé C, Hollfelder F. Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution. Chemistry & Biology. 19: 1001-9. PMID 22921067 DOI: 10.1016/J.Chembiol.2012.06.009 |
0.743 |
|
| 2012 |
Kaltenbach M, Devenish SR, Hollfelder F. A simple method to evaluate the biochemical compatibility of oil/surfactant mixtures for experiments in microdroplets. Lab On a Chip. 12: 4185-92. PMID 22885600 DOI: 10.1039/C2Lc40281E |
0.333 |
|
| 2012 |
Babtie AC, Lima MF, Kirby AJ, Hollfelder F. Kinetic and computational evidence for an intermediate in the hydrolysis of sulfonate esters. Organic & Biomolecular Chemistry. 10: 8095-101. PMID 22832951 DOI: 10.1039/C2Ob25699A |
0.515 |
|
| 2012 |
Stein V, Kaltenbach M, Hollfelder F. Assembling linear DNA templates for in vitro transcription and translation. Methods in Molecular Biology (Clifton, N.J.). 815: 67-78. PMID 22130984 DOI: 10.1007/978-1-61779-424-7_6 |
0.712 |
|
| 2012 |
Delley RJ, Bandyopadhyay S, Fox MA, Schliehe C, Hodgson DR, Hollfelder F, Kirby AJ, O'Donoghue AC. peri-Dimethylamino substituent effects on proton transfer at carbon in α-naphthylacetate esters: a model for mandelate racemase. Organic & Biomolecular Chemistry. 10: 590-6. PMID 22108698 DOI: 10.1039/C1Ob06525D |
0.604 |
|
| 2012 |
Kaltenbach M, Hollfelder F. SNAP display: in vitro protein evolution in microdroplets. Methods in Molecular Biology (Clifton, N.J.). 805: 101-11. PMID 22094803 DOI: 10.1007/978-1-61779-379-0_7 |
0.352 |
|
| 2011 |
Villiers B, Hollfelder F. Directed evolution of a gatekeeper domain in nonribosomal peptide synthesis. Chemistry & Biology. 18: 1290-9. PMID 22035798 DOI: 10.1016/J.Chembiol.2011.06.014 |
0.323 |
|
| 2011 |
Kaltenbach M, Stein V, Hollfelder F. SNAP dendrimers: multivalent protein display on dendrimer-like DNA for directed evolution. Chembiochem : a European Journal of Chemical Biology. 12: 2208-16. PMID 21780273 DOI: 10.1002/Cbic.201100240 |
0.726 |
|
| 2011 |
Huebner AM, Abell C, Huck WT, Baroud CN, Hollfelder F. Monitoring a reaction at submillisecond resolution in picoliter volumes. Analytical Chemistry. 83: 1462-8. PMID 21244017 DOI: 10.1021/Ac103234A |
0.324 |
|
| 2010 |
Schaerli Y, Stein V, Spiering MM, Benkovic SJ, Abell C, Hollfelder F. Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification. Nucleic Acids Research. 38: e201. PMID 20921065 DOI: 10.1093/Nar/Gkq795 |
0.764 |
|
| 2010 |
Kintses B, van Vliet LD, Devenish SR, Hollfelder F. Microfluidic droplets: new integrated workflows for biological experiments. Current Opinion in Chemical Biology. 14: 548-55. PMID 20869904 DOI: 10.1016/J.Cbpa.2010.08.013 |
0.721 |
|
| 2010 |
Theberge AB, Courtois F, Schaerli Y, Fischlechner M, Abell C, Hollfelder F, Huck WT. Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angewandte Chemie (International Ed. in English). 49: 5846-68. PMID 20572214 DOI: 10.1002/Anie.200906653 |
0.713 |
|
| 2010 |
van Loo B, Jonas S, Babtie AC, Benjdia A, Berteau O, Hyvönen M, Hollfelder F. An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily. Proceedings of the National Academy of Sciences of the United States of America. 107: 2740-5. PMID 20133613 DOI: 10.1073/Pnas.0903951107 |
0.798 |
|
| 2010 |
Babtie A, Tokuriki N, Hollfelder F. What makes an enzyme promiscuous? Current Opinion in Chemical Biology. 14: 200-7. PMID 20080434 DOI: 10.1016/J.Cbpa.2009.11.028 |
0.383 |
|
| 2010 |
Villiers BR, Stein V, Hollfelder F. USER friendly DNA recombination (USERec): a simple and flexible near homology-independent method for gene library construction. Protein Engineering, Design & Selection : Peds. 23: 1-8. PMID 19897542 DOI: 10.1093/Protein/Gzp063 |
0.722 |
|
| 2010 |
Hollfelder F, Kirby AJ, Tawfik DS, Kikuchi K, Hilvert D. ChemInform Abstract: Characterization of Proton-Transfer Catalysis by Serum Albumins Cheminform. 31: no-no. DOI: 10.1002/chin.200018038 |
0.386 |
|
| 2010 |
Theberge A, Courtois F, Schaerli Y, Fischlechner M, Abell C, Hollfelder F, Huck W. Mikrotröpfchen in Mikrofluidiksystemen: eine Technik für Entdeckungen in der Chemie und Biologie Angewandte Chemie. 122: 5982-6005. DOI: 10.1002/Ange.200906653 |
0.664 |
|
| 2009 |
Schaerli Y, Hollfelder F. The potential of microfluidic water-in-oil droplets in experimental biology. Molecular Biosystems. 5: 1392-404. PMID 20023716 DOI: 10.1039/B907578J |
0.718 |
|
| 2009 |
Robinson T, Schaerli Y, Wootton R, Hollfelder F, Dunsby C, Baldwin G, Neil M, French P, deMello A. Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging. Lab On a Chip. 9: 3437-41. PMID 19904413 DOI: 10.1039/B913293G |
0.685 |
|
| 2009 |
Shim JU, Olguin LF, Whyte G, Scott D, Babtie A, Abell C, Huck WT, Hollfelder F. Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. Journal of the American Chemical Society. 131: 15251-6. PMID 19799429 DOI: 10.1021/Ja904823Z |
0.327 |
|
| 2009 |
Stein V, Hollfelder F. An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Research. 37: e122. PMID 19617373 DOI: 10.1093/Nar/Gkp589 |
0.721 |
|
| 2009 |
Hufnagel H, Hakim P, Lima A, Hollfelder F. Fluid phase endocytosis contributes to transfection of DNA by PEI-25. Molecular Therapy : the Journal of the American Society of Gene Therapy. 17: 1411-7. PMID 19532143 DOI: 10.1038/Mt.2009.121 |
0.315 |
|
| 2009 |
Damean N, Olguin LF, Hollfelder F, Abell C, Huck WT. Simultaneous measurement of reactions in microdroplets filled by concentration gradients. Lab On a Chip. 9: 1707-13. PMID 19495454 DOI: 10.1039/B821021G |
0.306 |
|
| 2009 |
Babtie AC, Bandyopadhyay S, Olguin LF, Hollfelder F. Efficient catalytic promiscuity for chemically distinct reactions. Angewandte Chemie (International Ed. in English). 48: 3692-4. PMID 19373810 DOI: 10.1002/Anie.200805843 |
0.56 |
|
| 2009 |
Courtois F, Olguin LF, Whyte G, Theberge AB, Huck WT, Hollfelder F, Abell C. Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays. Analytical Chemistry. 81: 3008-16. PMID 19284775 DOI: 10.1021/Ac802658N |
0.342 |
|
| 2009 |
Villiers BR, Hollfelder F. Mapping the limits of substrate specificity of the adenylation domain of TycA. Chembiochem : a European Journal of Chemical Biology. 10: 671-82. PMID 19189362 DOI: 10.1002/Cbic.200800553 |
0.357 |
|
| 2009 |
Golicnik M, Olguin LF, Feng G, Baxter NJ, Waltho JP, Williams NH, Hollfelder F. Kinetic analysis of beta-phosphoglucomutase and its inhibition by magnesium fluoride. Journal of the American Chemical Society. 131: 1575-88. PMID 19132841 DOI: 10.1021/Ja806421F |
0.55 |
|
| 2009 |
Schaerli Y, Wootton RC, Robinson T, Stein V, Dunsby C, Neil MA, French PM, Demello AJ, Abell C, Hollfelder F. Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. Analytical Chemistry. 81: 302-6. PMID 19055421 DOI: 10.1021/Ac802038C |
0.769 |
|
| 2009 |
Jonas S, Hollfelder F. Mapping catalytic promiscuity in the alkaline phosphatase superfamily Pure and Applied Chemistry. 81: 731-742. DOI: 10.1351/Pac-Con-08-10-20 |
0.735 |
|
| 2009 |
Courtois F, Huebner A, Stein V, Olguin L, Hollfelder F. Towards biological experimentation in microfludic microdroplets | Vers l'expérimentation biologique dans des gouttelettes en microfluidique Houille Blanche. 127-133. DOI: 10.1051/Lhb/2009065 |
0.701 |
|
| 2009 |
Babtie AC, Bandyopadhyay S, Olguin LF, Hollfelder F. Effiziente katalytische Promiskuität für chemisch unterschiedliche Reaktionen Angewandte Chemie. 121: 3746-3749. DOI: 10.1002/Ange.200805843 |
0.442 |
|
| 2008 |
Olguin LF, Askew SE, O'Donoghue AC, Hollfelder F. Efficient catalytic promiscuity in an enzyme superfamily: an arylsulfatase shows a rate acceleration of 10(13) for phosphate monoester hydrolysis. Journal of the American Chemical Society. 130: 16547-55. PMID 19554727 DOI: 10.1021/Ja8047943 |
0.334 |
|
| 2008 |
Kirby AJ, Hollfelder F. Biochemistry: Enzymes under the nanoscope. Nature. 456: 45-7. PMID 18987728 DOI: 10.1038/456045A |
0.534 |
|
| 2008 |
Leemhuis H, Nightingale KP, Hollfelder F. Directed evolution of a histone acetyltransferase--enhancing thermostability, whilst maintaining catalytic activity and substrate specificity. The Febs Journal. 275: 5635-47. PMID 18959749 DOI: 10.1111/J.1742-4658.2008.06689.X |
0.348 |
|
| 2008 |
Jonas S, van Loo B, Hyvönen M, Hollfelder F. A new member of the alkaline phosphatase superfamily with a formylglycine nucleophile: structural and kinetic characterisation of a phosphonate monoester hydrolase/phosphodiesterase from Rhizobium leguminosarum. Journal of Molecular Biology. 384: 120-36. PMID 18793651 DOI: 10.1016/J.Jmb.2008.08.072 |
0.782 |
|
| 2008 |
Van Vliet LD, Chapman MR, Avenier F, Kitson CZ, Hollfelder F. Relating chemical and biological diversity space: a tunable system for efficient gene transfection. Chembiochem : a European Journal of Chemical Biology. 9: 1960-7. PMID 18655081 DOI: 10.1002/Cbic.200800003 |
0.354 |
|
| 2008 |
Huebner A, Sharma S, Srisa-Art M, Hollfelder F, Edel JB, Demello AJ. Microdroplets: a sea of applications? Lab On a Chip. 8: 1244-54. PMID 18651063 DOI: 10.1039/B806405A |
0.325 |
|
| 2008 |
Huebner A, Olguin LF, Bratton D, Whyte G, Huck WT, de Mello AJ, Edel JB, Abell C, Hollfelder F. Development of quantitative cell-based enzyme assays in microdroplets. Analytical Chemistry. 80: 3890-6. PMID 18399662 DOI: 10.1021/Ac800338Z |
0.388 |
|
| 2008 |
Baxter NJ, Blackburn GM, Marston JP, Hounslow AM, Cliff MJ, Bermel W, Williams NH, Hollfelder F, Wemmer DE, Waltho JP. Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes. Journal of the American Chemical Society. 130: 3952-8. PMID 18318536 DOI: 10.1021/Ja078000N |
0.55 |
|
| 2008 |
Courtois F, Olguin LF, Whyte G, Bratton D, Huck WT, Abell C, Hollfelder F. An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. Chembiochem : a European Journal of Chemical Biology. 9: 439-46. PMID 18232037 DOI: 10.1002/Cbic.200700536 |
0.354 |
|
| 2007 |
Stein V, Sielaff I, Johnsson K, Hollfelder F. A covalent chemical genotype-phenotype linkage for in vitro protein evolution. Chembiochem : a European Journal of Chemical Biology. 8: 2191-4. PMID 17948318 DOI: 10.1002/Cbic.200700459 |
0.683 |
|
| 2007 |
Avenier F, Domingos JB, Vliet LD, Hollfelder F. Polyethylene imine derivatives ('synzymes') accelerate phosphate transfer in the absence of metal. Journal of the American Chemical Society. 129: 7611-9. PMID 17530755 DOI: 10.1021/Ja069095G |
0.675 |
|
| 2007 |
Van Vliet LD, Ellis T, Foley PJ, Liu L, Pfeffer FM, Russell RA, Warrener RN, Hollfelder F, Waring MJ. Molecular recognition of DNA by rigid [N]-polynorbornane-derived bifunctional intercalators: synthesis and evaluation of their binding properties. Journal of Medicinal Chemistry. 50: 2326-40. PMID 17429957 DOI: 10.1021/Jm0613020 |
0.321 |
|
| 2007 |
Otten LG, Schaffer ML, Villiers BR, Stachelhaus T, Hollfelder F. An optimized ATP/PP(i)-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis. Biotechnology Journal. 2: 232-40. PMID 17294409 DOI: 10.1002/Biot.200600220 |
0.338 |
|
| 2007 |
Stein V, Sielaff I, Johnsson K, Hollfelder F. Cover Picture: A Covalent Chemical Genotype–Phenotype Linkage for in vitro Protein Evolution (ChemBioChem 18/2007) Chembiochem. 8: 2173-2173. DOI: 10.1002/Cbic.200790064 |
0.697 |
|
| 2006 |
Baxter NJ, Olguin LF, Golicnik M, Feng G, Hounslow AM, Bermel W, Blackburn GM, Hollfelder F, Waltho JP, Williams NH. A Trojan horse transition state analogue generated by MgF3- formation in an enzyme active site. Proceedings of the National Academy of Sciences of the United States of America. 103: 14732-7. PMID 16990434 DOI: 10.1073/Pnas.0604448103 |
0.568 |
|
| 2005 |
Leemhuis H, Stein V, Griffiths AD, Hollfelder F. New genotype-phenotype linkages for directed evolution of functional proteins. Current Opinion in Structural Biology. 15: 472-8. PMID 16043338 DOI: 10.1016/J.Sbi.2005.07.006 |
0.703 |
|
| 2005 |
Kirby AJ, Gesser JC, Hollfelder F, Priebe JP, Nome F. Intramolecular general acid catalysis of sulfate transfer - Nucleophilic attack by oxyanions on the so3 - group Canadian Journal of Chemistry. 83: 1629-1636. DOI: 10.1139/v05-172 |
0.578 |
|
| 2001 |
Hollfelder F, Kirby AJ, Tawfik DS. On the magnitude and specificity of medium effects in enzyme-like catalysts for proton transfer. The Journal of Organic Chemistry. 66: 5866-74. PMID 11511264 DOI: 10.1021/Jo015723V |
0.579 |
|
| 2001 |
Zamble DB, Miller DA, Heddle JG, Maxwell A, Walsh CT, Hollfelder F. In vitro characterization of DNA gyrase inhibition by microcin B17 analogs with altered bisheterocyclic sites. Proceedings of the National Academy of Sciences of the United States of America. 98: 7712-7. PMID 11427730 DOI: 10.1073/Pnas.141225698 |
0.743 |
|
| 2001 |
Heddle JG, Blance SJ, Zamble DB, Hollfelder F, Miller DA, Wentzell LM, Walsh CT, Maxwell A. The antibiotic microcin B17 is a DNA gyrase poison: characterisation of the mode of inhibition. Journal of Molecular Biology. 307: 1223-34. PMID 11292337 DOI: 10.1006/Jmbi.2001.4562 |
0.724 |
|
| 2000 |
Kirby AJ, Hollfelder F, Tawfik DS. Nonspecific catalysis by protein surfaces. Applied Biochemistry and Biotechnology. 83: 173-80; discussion 1. PMID 10826958 DOI: 10.1385/Abab:83:1-3:173 |
0.575 |
|
| 2000 |
Pérez-Juste J, Hollfelder F, Kirby AJ, Engberts JB. Vesicles accelerate proton transfer from carbon up to 850-fold. Organic Letters. 2: 127-30. PMID 10814263 DOI: 10.1021/Ol991215K |
0.454 |
|
| 2000 |
Hollfelder F, Kirby AJ, Tawfik DS, Kikuchi K, Hilvert D. Characterization of proton-transfer catalysis by serum albumins Journal of the American Chemical Society. 122: 1022-1029. DOI: 10.1021/Ja993471Y |
0.555 |
|
| 1999 |
Zhang YL, Hollfelder F, Gordon SJ, Chen L, Keng YF, Wu L, Herschlag D, Zhang ZY. Impaired transition state complementarity in the hydrolysis of O- arylphosphorothioates by protein-tyrosine phosphatases Biochemistry. 38: 12111-12123. PMID 10508416 DOI: 10.1021/Bi990836I |
0.545 |
|
| 1997 |
Hollfelder F, Kirby AJ, Tawfik DS. Efficient catalysis of proton transfer by synzymes Journal of the American Chemical Society. 119: 9578-9579. DOI: 10.1021/Ja971970Y |
0.344 |
|
| 1997 |
Genre-Grandpierre A, Tellier C, Loirat M, Blanchard D, Hodgson DR, Hollfelder F, Kirby AJ. Catalysis of the Kemp elimination by antibodies elicited against a cationic hapten Bioorganic & Medicinal Chemistry Letters. 7: 2497-2502. DOI: 10.1016/S0960-894X(97)10003-8 |
0.506 |
|
| 1996 |
Hollfelder F, Kirby AJ, Tawfik DS. Off-the-shelf proteins that rival tailor-made antibodies as catalysts. Nature. 383: 60-2. PMID 8779715 DOI: 10.1038/383060A0 |
0.576 |
|
| 1995 |
Hollfelder F, Herschlag D. The nature of the transition state for enzyme-catalyzed phosphoryl transfer. Hydrolysis of O-aryl phosphorothioates by alkaline phosphatase. Biochemistry. 34: 12255-64. PMID 7547968 DOI: 10.1021/Bi00038A021 |
0.579 |
|
| 1994 |
Dalby KN, Kirby AJ, Hollfelder F. Simple but efficient models for nuclease catalysis Pure and Applied Chemistry. 66: 687-694. DOI: 10.1351/Pac199466040687 |
0.724 |
|
| 1994 |
Chandler AJ, Hollfelder F, Kirby AJ, O'Carroll F, Strömberg R. Models for enzyme-catalysed phosphate transfer: comparisons of reactivity towards a neighbouring hydroxy group for phosphodiester anions and acids. General base catalysis of the cyclisation of a hydroxyalkyl phosphate triester Journal of the Chemical Society-Perkin Transactions 1. 327-333. DOI: 10.1039/P29940000327 |
0.515 |
|
| 1993 |
Dalby KN, Kirby AJ, Hollfelder F. Models for nuclease catalysis: Mechanisms for general acid catalysis of the rapid intramolecular displacement of methoxide from a phosphate diester Journal of the Chemical Society-Perkin Transactions 1. 1269-1281. DOI: 10.1039/P29930001269 |
0.539 |
|
| 1993 |
Dalby KN, Kirby AJ, Hollfelder F. Models for nuclease catalysis: Mechanisms for general acid catalysis of the rapid intramolecular displacement of methoxide from a phosphate diester Journal of the Chemical Society, Perkin Transactions 2. 1269-1281. |
0.66 |
|
| 1992 |
Dalby KN, Hollfelder F, Kirby AJ. Electrostatic catalysis of the hydrolysis of a phosphate diester in water Journal of the Chemical Society, Chemical Communications. 1770-1771. DOI: 10.1039/C39920001770 |
0.711 |
|
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