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According to our matching algorithm, Surinder Chopra is the likely recipient of the following grants.
Years |
Recipients |
Code |
Title / Keywords |
Matching score |
2004 — 2011 |
Chopra, Surinder |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Analysis of a Dominant Epigenetic Modifier of Pericarp Color1 (P1) Gene of Maize @ Pennsylvania State Univ University Park
Chopra Abstract
The investigator has previously characterized the expression pattern and gene structure of alleles of the maize p1 (pericarp color1) gene. P1 is a myb type of transcription factor and controls the biosynthesis of red flavonoid pigments called phlobaphenes. Previous findings suggest that allelic expression at the p1 locus is a function of the gene structure and gene organization, which is further regulated by epigenetic mechanisms. The investigator has now characterized a dominant modifier called Ufo1 (Unstable factor for orange1). In the absence of Ufo1, plants of P1-wr (white pericarp & red cob glumes) allele produce ears with colorless kernel pericarp and red cobs and this phenotype is highly stable. In the presence of Ufo1, the P1-wr confers variably enhanced kernel pericarp, cob and plant pigmentation. Preliminary expression studies demonstrate that the gain of pigmentation is associated with increased steady state levels of p1 transcript that is correlated with decrease in DNA methylation of promoter and coding sequence of the P1-wr. Further experiments will investigate Ufo1 induced de-regulation of a well-programmed expression profile via transient DNA methylation changes of p1 alleles. Specifically, 1) using bisulfite sequencing methods, this project will identify specific type(s) and position(s) of DNA methylation modifications in the p1 sequence in the presence of Ufo1, 2) genes identified from the microarray analysis of Ufo1 versus ufo1 genotype RNA will be further tested for their expression and functionality and 3) to clone the Ufo1 gene, the research project will begin to develop plant populations to fine map the Ufo1 factor within the known crude position on the short arm of chromosome 10. In the longer run, this study will lead to the identification of molecular marks and mechanisms that may regulate stability of transgenes and natural alleles. Graduate students and undergraduates students will perform the majority of the research on this project. This project will provide training opportunities in epigenetics, as well as in functional genomics.
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1 |
2011 — 2017 |
Chopra, Surinder |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Collaborative Research: the Epigenetic Regulatory Role of the Maize Ufo1 Gene @ Pennsylvania State Univ University Park
Intellectual Merit: One of the mechanisms that allow diversification of form and function is epigenetic regulation. Epigenetic changes are alterations in the DNA or protein components of chromatin that affect gene expression. Epigenetic modifications that are of transient nature are widespread in the plant kingdom, but are rarely isolated or identified because the associated phenotypes are not easy to see and the modifications are not stable from generation to generation. Using the pericarp color1 (p1) gene and its red pigmentation as a marker in maize, allelic variation at the p1 locus has been shown to be a function of gene structure and organization, which is further regulated by epigenetic mechanisms. To understand the basis of allelic diversity modulated through epigenetic regulation of gene expression, molecular and genetic characterization of a dominant mutation called Ufo1 (Unstable factor for orange1) has been performed. Plants with a particular p1 allele, called P1-wr, produce ears with colorless kernel pericarps and red cob glumes, and this silent pericarp phenotype is stable; however, in the presence of Ufo1 the P1-wr allele confers variably enhanced pigmentation phenotypes in several tissues. Expression studies demonstrated that the gain of pigmentation is associated with increased steady state levels of P1 transcript. Enhanced P1 expression in P1-wr;Ufo1 plants was shown to correlate with a decrease in DNA methylation at the promoter and coding sequence of the P1-wr allele. In the current project, map based cloning of ufo1 gene will be carried out. New alleles of ufo1 will be generated using transposon mutagenesis. Evidence for Ufo1-mediated effects on epigenetic regulation will be analyzed using high throughput sequencing methods for global profiling of chromatin, transcript (mRNA and small RNA), and DNA methylation of Ufo1 mutant and wild type plants. A database will be developed to visualize and analyze data generated from ChIP-seq, RNA-seq and bisulfite sequencing. The results will establish the role of the Ufo1 mutation (and wild type ufo1 gene) in effecting global changes in transposons, DNA methylation, histone modifications and RNA profiles. Because Ufo1 does not appear to act in the same way as other known epigenetic regulators in maize, this project will expand understanding of how overlapping epigenetic pathways regulate gene expression in this important crop plant.
Broader Impacts: This project will provide training opportunities in plant epigenetics and study of allelic variation of plant genes. The collaboration between Pennsylvania State University and the University Delaware will enhance professional interactions in the area of plant biology and bioinformatics. Specifically, two postdoctoral fellows and two graduate students will be cross-trained in computational biological aspects and epigenetic gene regulation. Undergraduate students, including women and minority students, will learn classical and cutting-edge plant biology techniques that are used understand and dissect the molecular basis of regulation of tissue-specific gene expression. In addition, high school students and teachers will participate in a summer biotechnology workshop to learn gene expression techniques in maize.
This project is co-funded by the Genetic Mechanisms Program in the Division of Molecular and Cellular Biosciences and by the Plant Genome Research Program in the Division of Integrative Organismal Systems.
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