Michael Ned Margolies, M.D. - Publications

Affiliations: 
Surgery Massachusetts General Hospital, Boston, MA 

60 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2002 Krykbaev RA, Tsantili P, Jeffrey PD, Margolies MN. Modifying specificity of antidigoxin antibodies using insertional mutagenesis. Protein Science : a Publication of the Protein Society. 11: 2899-908. PMID 12441388 DOI: 10.1110/ps.0223402  1
2002 Short MK, Krykbaev RA, Jeffrey PD, Margolies MN. Complementary combining site contact residue mutations of the anti-digoxin Fab 26-10 permit high affinity wild-type binding. The Journal of Biological Chemistry. 277: 16365-70. PMID 11854275 DOI: 10.1074/jbc.M110444200  1
2001 Parhami-Seren B, Viswanathan M, Strong RK, Margolies MN. Structural analysis of mutants of high-affinity and low-affinity p-azophenylarsonate-specific antibodies generated by alanine scanning of heavy chain complementarity-determining region 2 Journal of Immunology. 167: 5129-5135. PMID 11673524  1
2001 Short MK, Jeffrey PD, Demirjian A, Margolies MN. A single H:CDR3 residue in the anti-digoxin antibody 26-10 modulates specificity for C16-substituted digoxin analogs. Protein Engineering. 14: 287-96. PMID 11391021 DOI: 10.1093/protein/14.4.287  1
2001 Krykbaev RA, Liu WR, Jeffrey PD, Margolies MN. Phage display-selected sequences of the heavy-chain CDR3 loop of the anti-digoxin antibody 26-10 define a high affinity binding site for position 16-substituted analogs of digoxin. The Journal of Biological Chemistry. 276: 8149-58. PMID 11060305 DOI: 10.1074/jbc.M008108200  1
2000 Roy P, Roth CM, Margolies MN, Yarmush ML. Aromatic residues mediate the pressure-induced association of digoxigenin and antibody 26-10 Biophysical Chemistry. 83: 171-177. PMID 10647847 DOI: 10.1016/S0301-4622(99)00139-8  1
1999 Roy P, Roth CM, Margolies MN, Yarmush ML. Effect of pressure on antigen-antibody complexes: Modulation by temperature and ionic strength Molecular Immunology. 36: 1149-1158. PMID 10698317 DOI: 10.1016/S0161-5890(99)00133-9  1
1997 Kasturi R, McLean LR, Margolies MN, Ball WJ. High affinity anti-digoxin antibodies as model receptors for cardiac glycosides. Comparisons with Na+,K+-ATPase Annals of the New York Academy of Sciences. 834: 634-636. PMID 9405877 DOI: 10.1111/j.1749-6632.1997.tb52335.x  1
1997 Gill DS, Wong YW, Margolies MN. Differences in sequence-specific expression of two anti-arsonate Fabs in E. coli Biotechnology Progress. 13: 692-694. PMID 9336990 DOI: 10.1021/bp970083w  1
1996 Huston JS, Margolies MN, Haber E. Antibody binding sites. Advances in Protein Chemistry. 49: 329-450. PMID 8908302  1
1995 Wong YW, Kussie PH, Parhami-Seren B, Margolies MN. Modulation of antibody affinity by an engineered amino acid substitution. Journal of Immunology (Baltimore, Md. : 1950). 154: 3351-8. PMID 7897218  1
1995 Jeffrey PD, Schildbach JF, Chang CY, Kussie PH, Margolies MN, Sheriff S. Structure and specificity of the anti-digoxinantibody 40-50 Journal of Molecular Biology. 248: 344-360. PMID 7739045  1
1995 Short MK, Jeffrey PD, Kwong RF, Margolies MN. Contribution of antibody heavy chain CDR1 to digoxin binding analyzed by random mutagenesis of phage-displayed Fab 26-10. The Journal of Biological Chemistry. 270: 28541-50. PMID 7499368 DOI: 10.1074/jbc.270.48.28541  1
1994 Chang CY, Shih H, Jeffrey PD, Margolies MN, Sheriff S. Crystallization and preliminary X-ray analysis of anti-digoxin antibodies. Acta Crystallographica. Section D, Biological Crystallography. 50: 915-7. PMID 15299362 DOI: 10.1107/S0907444994004841  1
1994 Kussie PH, Parhami-Seren B, Wysocki LJ, Margolies MN. A single engineered amino acid substitution changes antibody fine specificity. Journal of Immunology (Baltimore, Md. : 1950). 152: 146-52. PMID 8254187  1
1993 Schillbach JF, Near RI, Bruccoleri RE, Haber E, Jeffrey PD, Novotny J, Sheriff S, Margolies MN. Modulation of antibody affinity by a non-contact residue. Protein Science : a Publication of the Protein Society. 2: 206-14. PMID 8443598 DOI: 10.1002/pro.5560020209  1
1993 Parhami-Seren B, Kussie PH, Strong RK, Margolies MN. Conservation of binding site geometry among p-azophenylarsonate-specific antibodies Journal of Immunology. 150: 1829-1837. PMID 8436818  1
1993 Panka DJ, Very DL, Jacobson BA, Kussie PH, Parhami-Seren B, Margolies MN, Marshak-Rothstein A. Defining the structural correlates responsible for loss of arsonate affinity in an IdCR antibody isolated from an autoimmune mouse Molecular Immunology. 30: 1013-1020. PMID 8350871 DOI: 10.1016/0161-5890(93)90126-V  1
1993 Jeffrey PD, Strong RK, Sieker LC, Chang CY, Campbell RL, Petsko GA, Haber E, Margolies MN, Sheriff S. 26-10 Fab-digoxin complex: affinity and specificity due to surface complementarity. Proceedings of the National Academy of Sciences of the United States of America. 90: 10310-4. PMID 8234291 DOI: 10.1073/pnas.90.21.10310  1
1993 Ping J, Schildbach JF, Shaw SY, Quertermous T, Novotny J, Bruccoleri R, Margolies MN. Effect of heavy chain signal peptide mutations and NH2-terminal chain length on binding of anti-digoxin antibodies. The Journal of Biological Chemistry. 268: 23000-7. PMID 8226814  1
1993 Schildbach JF, Near RI, Bruccoleri RE, Haber E, Jeffrey PD, Ng SC, Novotny J, Sheriff S, Margolies MN. Heavy chain position 50 is a determinant of affinity and specificity for the anti-digoxin antibody 26-10. The Journal of Biological Chemistry. 268: 21739-47. PMID 7691815  1
1992 Shaw SY, Margolies MN. A spontaneous variant of an antidigoxin hybridoma antibody with increased affinity arises from a heavy chain signal peptide mutation. Molecular Immunology. 29: 525-9. PMID 1565100 DOI: 10.1016/0161-5890(92)90010-u  1
1991 Strong RK, Petsko GA, Sharon J, Margolies MN. Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 2. Structural basis of hapten binding and idiotypy Biochemistry. 30: 3749-3757. PMID 2015230  1
1991 Strong RK, Campbell R, Rose DR, Petsko GA, Sharon J, Margolies MN. Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 1. X-ray crystallography, site-directed mutagenesis, and modeling of the complex with hapten Biochemistry. 30: 3739-3748. PMID 2015229  1
1991 Schildbach JF, Panka DJ, Parks DR, Jager GC, Novotny J, Herzenberg LA, Mudgett-Hunter M, Bruccoleri RE, Haber E, Margolies MN. Altered hapten recognition by two anti-digoxin hybridoma variants due to variable region point mutations. The Journal of Biological Chemistry. 266: 4640-7. PMID 1999439  1
1990 Rose DR, Strong RK, Margolies MN, Gefter ML, Petsko GA. Crystal structure of the antigen-binding fragment of the murine anti-arsonate monoclonal antibody 36-71 at 2.9-Å resolution Proceedings of the National Academy of Sciences of the United States of America. 87: 338-342. PMID 2296590  1
1990 Near RI, Ng SC, Mudgett-Hunter M, Hudson NW, Margolies MN, Seidman JG, Haber E, Jacobson MA. Heavy and light chain contributions to antigen binding in an anti-digoxin chain recombinant antibody produced by transfection of cloned anti-digoxin antibody genes. Molecular Immunology. 27: 901-9. PMID 2120577 DOI: 10.1016/0161-5890(90)90157-U  1
1990 Hudson NW, Bruccoleri RE, Steinrauf LK, Hamilton JA, Mudgett-Hunter M, Margolies MN. A V kappa-J kappa junctional change in an antidigoxin recombinant antibody destroys digoxin-binding activity. Journal of Immunology (Baltimore, Md. : 1950). 145: 2718-24. PMID 2120338  1
1989 Becker ML, Near R, Mudgett-Hunter M, Margolies MN, Kubo RT, Kaye J, Hedrick SM. Expression of a hybrid immunoglobulin-T cell receptor protein in transgenic mice. Cell. 58: 911-21. PMID 2528411 DOI: 10.1016/0092-8674(89)90943-4  1
1988 Mikoryak CA, Margolies MN, Steiner LA. J chain in Rana catesbeiana high molecular weight Ig Journal of Immunology. 140: 4279-4285. PMID 3131430  1
1988 Panka DJ, Mudgett-Hunter M, Parks DR, Peterson LL, Herzenberg LA, Haber E, Margolies MN. Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies. Proceedings of the National Academy of Sciences of the United States of America. 85: 3080-4. PMID 3129726  1
1988 Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotný J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber E, Crea R. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America. 85: 5879-83. PMID 3045807  1
1987 Hudson NW, Mudgett-Hunter M, Panka DJ, Margolies MN. Immunoglobulin chain recombination among antidigoxin antibodies by hybridoma-hybridoma fusion. Journal of Immunology (Baltimore, Md. : 1950). 139: 2715-23. PMID 3655369  1
1987 Panka DJ, Margolies MN. Complete variable region sequences of five homologous high affinity anti-digoxin antibodies. Journal of Immunology (Baltimore, Md. : 1950). 139: 2385-91. PMID 3655367  1
1985 Mudgett-Hunter M, Anderson W, Haber E, Margolies MN. Binding and structural diversity among high-affinity monoclonal anti-digoxin antibodies. Molecular Immunology. 22: 477-88. PMID 4033667  1
1985 Wysocki LJ, Margolies MN, Huang B, Nemazee DA, Wechsler DS, Sato VL, Smith JA, Gefter ML. Combinational diversity within variable regions bearing the predominant anti-p-azophenylarsonate idiotype of strain A mice. Journal of Immunology (Baltimore, Md. : 1950). 134: 2740-7. PMID 2982953  1
1984 Gefter ML, Margolies MN, Near RI, Wysocki LJ. Analysis of the anti-azobenzenearsonate response at the molecular level. Annales D'Immunologie. 135: 17-30. PMID 6712160  1
1984 Lechat P, Mudgett-Hunter M, Margolies MN, Haber E, Smith TW. Reversal of lethal digoxin toxicity in guinea pigs using monoclonal antibodies and Fab fragments. The Journal of Pharmacology and Experimental Therapeutics. 229: 210-3. PMID 6707937  1
1984 Near RI, Juszczak EC, Huang SY, Sicari SA, Margolies MN, Gefter ML. Expression and rearrangement of homologous immunoglobulin VH genes in two mouse strains. Proceedings of the National Academy of Sciences of the United States of America. 81: 2167-71. PMID 6326109  1
1984 Juszczak E, Near RI, Gefter ML, Margolies MN. Complete heavy and light chain variable region sequence of anti-arsonate monoclonal antibodies from BALB/c and A/J mice sharing the 36-60 idiotype are highly homologous. Journal of Immunology (Baltimore, Md. : 1950). 133: 2603-9. PMID 6207237  1
1983 Rose DR, Seaton BA, Petsko GA, Novotný J, Margolies MN, Locke E, Haber E. Crystallization of the Fab fragment of a monoclonal anti-digoxin antibody and its complex with digoxin. Journal of Molecular Biology. 165: 203-6. PMID 6842605 DOI: 10.1016/S0022-2836(83)80252-6  1
1983 Margolies MN, Juszczak EC, Near R, Marshak-Rothstein A, Rothstein TL, Sato VL, Siekevitz M, Smith JA, Wysocki LJ, Gefter ML. Structural correlates of idiotypy in the arsonate system. Annals of the New York Academy of Sciences. 418: 48-64. PMID 6424532  1
1983 Mudgett-Hunter M, Budzik GP, Donahoe PK, Khaw BA, Margolies MN, Ridgeway EC, Haber E. Monoclonal antibodies as physiologic probes. Basic Life Sciences. 25: 101-28. PMID 6190474  1
1982 Hunter MM, Margolies MN, Ju A, Haber E. High-affinity monoclonal antibodies to the cardiac glycoside, digoxin. Journal of Immunology (Baltimore, Md. : 1950). 129: 1165-72. PMID 6179995  1
1981 Margolies MN, Marshak-Rothstein A, Gefter ML. Structural diversity among anti-p-azophenylarsonate monoclonal antibodies from A/J mice: Comparison of Id- and Id+ sequences Molecular Immunology. 18: 1065-1077. PMID 6801478 DOI: 10.1016/0161-5890(81)90022-5  1
1980 Marshak-Rothstein A, Siekevitz M, Margolies MN, Mudgett-Hunter M, Gefter ML. Hybridoma proteins expressing the predominant idiotype of the antiazophenylarsonate response of A/J mice Proceedings of the National Academy of Sciences of the United States of America. 77: 1120-1124. PMID 6767242  1
1980 Ehrlich PH, Matsueda GR, Margolies MN, Husain SS, Haber E. Isolation of an active heavy-chain variable domain from a homogeneous rabbit antibody by cathepsin B digestion of the aminoethylated heavy chain. Biochemistry. 19: 4091-6. PMID 6157405  1
1979 Ehrlich PH, Matsueda GR, Haber E, Margolies MN. Isolation and characterization of an active variable domain from a homogeneous rabbit antibody light chain. Biochemistry. 18: 4411-8. PMID 114216  1
1978 Cannon LE, Margolies MN, Haber E. Light chain variable region sequence of rabbit antipneumococcal type III polysaccharide antibody 3368. Biochemistry. 17: 3101-9. PMID 100133  1
1978 Ehrlich PH, Matsueda GR, Margolies MN, Haber E. Preparation of an active Fd fragment by cyanogen bromide cleavage of an IgG heavy chain from a homogeneous rabbit antibody. Immunochemistry. 15: 937-40. PMID 35462  1
1978 Zurawski VR, Novotný J, Haber E, Margolies MN. Antibodies of restricted heterogeneity directed against the cardiac glycoside digoxin. Journal of Immunology (Baltimore, Md. : 1950). 121: 122-9. PMID 27558  1
1977 Novotný J, FranÄ•k F, Margolies MN, Haber E. Amino acid sequence of normal (microheterogeneous) porcine immunoglobulin lambda chains. Biochemistry. 16: 3765-72. PMID 409425  1
1977 Haber E, Margolies MN, Cannon LE. Origins of antibody diversity: insight gained from amino acid sequence studies of elicited antibodies. Cold Spring Harbor Symposia On Quantitative Biology. 41: 647-59. PMID 19193  1
1976 Cannon LE, Margolies MN, Strosberg AD, Chen FW, Newell J, Haber E. Diversity among rabbit antibody light chain amino terminal sequences. Journal of Immunology (Baltimore, Md. : 1950). 117: 160-7. PMID 819580  1
1976 Chen FW, Cannon LE, Margolies MN, Strosberg AD, Haber E. Purification, specificity, and hypervariable region sequence of anti-pneumococcal polysaccharide antibodies elicited in a single rabbit. Journal of Immunology (Baltimore, Md. : 1950). 117: 807-13. PMID 8570  1
1975 Margolies MN, Cannon LE, Strosberg AD, Haber E. Diversity of light chain variable region sequences among rabbit antibodies elicited by the same antigens. Proceedings of the National Academy of Sciences of the United States of America. 72: 2180-4. PMID 1094456 DOI: 10.1073/pnas.72.6.2180  1
1975 Strosberg AD, Margolies MN, Haber E. The interdomain disulfide bond of a homogeneous rabbit pneumococcal antibody light chain. Journal of Immunology (Baltimore, Md. : 1950). 115: 1422-4. PMID 240892  1
1972 Strosberg AD, Fraser KJ, Margolies MN, Haber E. Amino acid sequence of rabbit pneumococcal antibody. I. Light-chain cysteine-containing peptides. Biochemistry. 11: 4978-85. PMID 4118103 DOI: 10.1021/bi00776a017  1
1971 Jaton JC, Waterfield MD, Margolies MN, Bloch KJ, Haber E. Variation in the primary structure of antibodies during the course of immunization. Biochemistry. 10: 1583-7. PMID 4103273 DOI: 10.1021/bi00785a012  1
1970 Jaton JC, Waterfield MD, Margolies MN, Haber E. Isolation and characterization of structurally homogeneous antibodies from antipneumococcal sera. Proceedings of the National Academy of Sciences of the United States of America. 66: 959-66. PMID 4393268 DOI: 10.1073/pnas.66.3.959  1
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