Karen S. Lyle, Ph.D.

Biochemical, catalytic, and spectroscopic studies of redox active enzymes; protein engineering

Brian G. Fox	grad student	2003	UW Madison
(Mechanism and function of soluble acyl -acyl carrier protein desaturase systems in fatty acid metabolism.)

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Sobrado P, Lyle KS, Kaul SP, et al. (2006) Identification of the binding region of the [2Fe-2S] ferredoxin in stearoyl-acyl carrier protein desaturase: insight into the catalytic complex and mechanism of action. Biochemistry. 45: 4848-58
Dyer DH, Lyle KS, Rayment I, et al. (2005) X-ray structure of putative acyl-ACP desaturase DesA2 from Mycobacterium tuberculosis H37Rv. Protein Science : a Publication of the Protein Society. 14: 1508-17
Fox BG, Lyle KS, Rogge CE. (2004) Reactions of the diiron enzyme stearoyl-acyl carrier protein desaturase. Accounts of Chemical Research. 37: 421-9
Lyle KS, Haas JA, Fox BG. (2003) Rapid-mix and chemical quench studies of ferredoxin-reduced stearoyl-acyl carrier protein desaturase. Biochemistry. 42: 5857-66
Lyle KS, Möenne-Loccoz P, Ai J, et al. (2000) Resonance Raman studies of the stoichiometric catalytic turnover of a substrate-stearoyl-acyl carrier protein delta(9) desaturase complex. Biochemistry. 39: 10507-13