Wesley R. Legant, Ph.D.

Bioengineering University of Pennsylvania, Philadelphia, PA, United States 
Tissue Microfabrication
"Wesley Legant"
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Christopher S. Chen grad student 2012 Penn (BME Tree)
 (Microscale measurements of cell and tissue mechanics in three dimensions.)
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Legant WR, Shao L, Grimm JB, et al. (2016) High-density three-dimensional localization microscopy across large volumes. Nature Methods
Yamashita N, Morita M, Legant WR, et al. (2015) Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus. Journal of Biomedical Optics. 20: 101206
Ritter AT, Asano Y, Stinchcombe JC, et al. (2015) Actin depletion initiates events leading to granule secretion at the immunological synapse. Immunity. 42: 864-76
Kural C, Akatay AA, Gaudin R, et al. (2015) Asymmetric formation of coated pits on dorsal and ventral surfaces at the leading edges of motile cells and on protrusions of immobile cells. Molecular Biology of the Cell. 26: 2044-53
Chen BC, Legant WR, Wang K, et al. (2014) Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution. Science (New York, N.Y.). 346: 1257998
Chen J, Zhang Z, Li L, et al. (2014) Single-molecule dynamics of enhanceosome assembly in embryonic stem cells. Cell. 156: 1274-85
Ramade A, Legant WR, Picart C, et al. (2014) Microfabrication of a platform to measure and manipulate the mechanics of engineered microtissues. Methods in Cell Biology. 121: 191-211
Grimm JB, Sung AJ, Legant WR, et al. (2013) Carbofluoresceins and carborhodamines as scaffolds for high-contrast fluorogenic probes. Acs Chemical Biology. 8: 1303-10
Khetan S, Guvendiren M, Legant WR, et al. (2013) Degradation-mediated cellular traction directs stem cell fate in covalently crosslinked three-dimensional hydrogels. Nature Materials. 12: 458-65
Legant WR, Choi CK, Miller JS, et al. (2013) Multidimensional traction force microscopy reveals out-of-plane rotational moments about focal adhesions. Proceedings of the National Academy of Sciences of the United States of America. 110: 881-6
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