John P. Guilinger, Ph.D.

Affiliations: 
2014 Biology, Molecular and Cellular Harvard University, Cambridge, MA, United States 
Area:
The Chemistry of Molecular Evolution
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"John Guilinger"
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David R. Liu grad student 2014 Harvard
 (Profiling and Improving the Specificity of Site-Specific Nucleases.)
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Publications

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Hubbard BP, Badran AH, Zuris JA, et al. (2015) Continuous directed evolution of DNA-binding proteins to improve TALEN specificity. Nature Methods. 12: 939-42
Zuris JA, Thompson DB, Shu Y, et al. (2015) Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nature Biotechnology. 33: 73-80
Pattanayak V, Guilinger JP, Liu DR. (2014) Determining the specificities of TALENs, Cas9, and other genome-editing enzymes. Methods in Enzymology. 546: 47-78
McDonald RI, Guilinger JP, Mukherji S, et al. (2014) Electrophilic activity-based RNA probes reveal a self-alkylating RNA for RNA labeling. Nature Chemical Biology. 10: 1049-54
Guilinger JP, Thompson DB, Liu DR. (2014) Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification Nature Biotechnology. 32: 577-582
Guilinger JP, Pattanayak V, Reyon D, et al. (2014) Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity Nature Methods. 11: 429-435
Pattanayak V, Guilinger JP, Liu DR. (2014) Determining the specificities of TALENs, cas9, and other genome-editing enzymes Methods in Enzymology. 546: 47-78
Pattanayak V, Lin S, Guilinger JP, et al. (2013) High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nature Biotechnology. 31: 839-43
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