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John A. Conrad, Ph.D.

Affiliations: 
2011 University of Michigan, Ann Arbor, Ann Arbor, MI 
Area:
Biochemistry
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Bruce A. Palfey grad student 2011 University of Michigan
 (The reactions of flavin-dependent thymidylate synthase from Thermotoga maritima.)

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Stack DE, Conrad JA, Mahmud B. (2018) Structural Identification and Kinetic Analysis of the in Vitro Products Formed by Reaction of Bisphenol A-3,4-quinone with N-Acetylcysteine and Glutathione. Chemical Research in Toxicology
Conrad JA, Ortiz-Maldonado M, Hoppe SW, et al. (2014) Detection of intermediates in the oxidative half-reaction of the FAD-dependent thymidylate synthase from Thermotoga maritima: carbon transfer without covalent pyrimidine activation. Biochemistry. 53: 5199-207
Youngblut M, Pauly DJ, Stein N, et al. (2014) Shewanella oneidensis cytochrome c nitrite reductase (ccNiR) does not disproportionate hydroxylamine to ammonia and nitrite, despite a strongly favorable driving force. Biochemistry. 53: 2136-44
Shah DD, Conrad JA, Moran GR. (2013) Intermediate partitioning kinetic isotope effects for the NIH shift of 4-hydroxyphenylpyruvate dioxygenase and the hydroxylation reaction of hydroxymandelate synthase reveal mechanistic complexity. Biochemistry. 52: 6097-107
Mishanina TV, Koehn EM, Conrad JA, et al. (2012) Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase. Journal of the American Chemical Society. 134: 4442-8
Shah DD, Conrad JA, Heinz B, et al. (2011) Evidence for the mechanism of hydroxylation by 4-hydroxyphenylpyruvate dioxygenase and hydroxymandelate synthase from intermediate partitioning in active site variants. Biochemistry. 50: 7694-704
Taylor MR, Conrad JA, Wahl D, et al. (2011) Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency. The Journal of Biological Chemistry. 286: 23054-62
He P, Conrad JA, Moran GR. (2010) The rate-limiting catalytic steps of hydroxymandelate synthase from Amycolatopsis orientalis. Biochemistry. 49: 1998-2007
Koehn EM, Fleischmann T, Conrad JA, et al. (2009) An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene Nature. 458: 919-923
Conrad JA, Moran GR. (2008) The Interaction of Hydroxymandelate Synthase with the 4-Hydroxyphenylpyruvate Dioxygenase Inhibitor: NTBC. Inorganica Chimica Acta. 361: 1197-1201
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