Florian Hollfelder

University of Cambridge, Cambridge, England, United Kingdom 
enzyme mechanism, directed evololution, physical-organic chemistry, microfluidics
"Florian Hollfelder"
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Daniel Herschlag research assistant 1994-1994 Stanford Medical School
Anthony J. Kirby grad student 1994-1998
Christopher T. Walsh post-doc 1998-2001 Harvard Medical School


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Stefanie Jonas grad student 2006-2009 ETH Zürich
Viktor Stein grad student 2007-2011 Technical University of Darmstadt
Yolanda Schaerli grad student 2008-2011 EPFL
Alexander Andre Cobb post-doc
Josiel Barbosa Domingos post-doc 2003-2005
Bert van Loo post-doc 2006-2011 Northumbria University
Balint Kintses post-doc 2009-2012 Cambridge (Cell Biology Tree)


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Kevin Dalby collaborator 1992-
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Scheele RA, Lindenburg LH, Petek M, et al. (2022) Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates. Nature Communications. 13: 844
Holstein JM, Gylstorff C, Hollfelder F. (2021) Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput. Acs Synthetic Biology
Lindenburg L, Hollfelder F. (2021) 'NAD-display': Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry. Angewandte Chemie (International Ed. in English)
Zurek PJ, Hours R, Schell U, et al. (2020) Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation. Lab On a Chip
Neun S, Zurek PJ, Kaminski TS, et al. (2020) Ultrahigh throughput screening for enzyme function in droplets. Methods in Enzymology. 643: 317-343
Emond S, Petek M, Kay EJ, et al. (2020) Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis. Nature Communications. 11: 3469
Kuo ST, Jahn RL, Cheng YJ, et al. (2020) Global fitness landscapes of the Shine-Dalgarno sequence. Genome Research
Lindenburg L, Huovinen T, van de Wiel K, et al. (2020) Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins. Nucleic Acids Research
van Loo B, Heberlein M, Mair P, et al. (2019) High-Throughput, Lysis-free Screening for Sulfatase Activity Using Autodisplay in Microdroplets. Acs Synthetic Biology
Neun S, Kaminski TS, Hollfelder F. (2019) Single-cell activity screening in microfluidic droplets. Methods in Enzymology. 628: 95-112
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