Florian Hollfelder

University of Cambridge, Cambridge, England, United Kingdom 
enzyme mechanism, directed evololution, physical-organic chemistry, microfluidics
"Florian Hollfelder"
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Daniel Herschlag research assistant 1994-1994 Stanford Medical School
Anthony J. Kirby grad student 1994-1998
Christopher T. Walsh post-doc 1998-2001 Harvard Medical School


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Stefanie Jonas grad student 2006-2009 ETH Zürich
Viktor Stein grad student 2007-2011 Technical University of Darmstadt
Yolanda Schaerli grad student 2008-2011 EPFL
Alexander Andre Cobb post-doc
Josiel Barbosa Domingos post-doc 2003-2005
Bert van Loo post-doc 2006-2011 Northumbria University
Balint Kintses post-doc 2009-2012 Cambridge (Cell Biology Tree)


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Kevin Dalby collaborator 1992-
Marko Hyvonen collaborator 2005- Cambridge (Computational Biology Tree)
BETA: Related publications


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Ladeveze S, Zurek PJ, Kaminski TS, et al. (2023) Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets. Acs Catalysis. 13: 10232-10243
Yam-Puc JC, Hosseini Z, Horner EC, et al. (2023) Age-associated B cells predict impaired humoral immunity after COVID-19 vaccination in patients receiving immune checkpoint blockade. Nature Communications. 14: 3292
Knyphausen P, Rangel Pereira M, Brear P, et al. (2023) Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture. Nature Communications. 14: 768
Neun S, Brear P, Campbell E, et al. (2022) Functional metagenomic screening identifies an unexpected β-glucuronidase. Nature Chemical Biology
Scheele RA, Lindenburg LH, Petek M, et al. (2022) Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates. Nature Communications. 13: 844
Lindenburg LH, Pantelejevs T, Gielen F, et al. (2021) Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats. Proceedings of the National Academy of Sciences of the United States of America. 118
Holstein JM, Gylstorff C, Hollfelder F. (2021) Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput. Acs Synthetic Biology
Lindenburg L, Hollfelder F. (2021) 'NAD-display': Ultrahigh-Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry. Angewandte Chemie (International Ed. in English)
Zurek PJ, Hours R, Schell U, et al. (2020) Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation. Lab On a Chip
Neun S, Zurek PJ, Kaminski TS, et al. (2020) Ultrahigh throughput screening for enzyme function in droplets. Methods in Enzymology. 643: 317-343
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