Jeff Nivala

University of Washington, Seattle, Seattle, WA 
nanopores, synthetic biology, protein engineering
"Jeff Nivala"
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David Baker research assistant 2008-2010 University of Washington
Mark Akeson grad student 2010-2014
George  M. Church post-doc 2014-2017 Harvard Medical School
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Nivala J, Mulroney L, Luan Q, et al. (2021) Unfolding and Translocation of Proteins Through an Alpha-Hemolysin Nanopore by ClpXP. Methods in Molecular Biology (Clifton, N.J.). 2186: 145-155
Palla M, Punthambaker S, Stranges B, et al. (2020) Multiplex Single-Molecule Kinetics of Nanopore-Coupled Polymerases. Acs Nano
Nivala J, Shipman SL, Church GM. (2018) Spontaneous CRISPR loci generation in vivo by non-canonical spacer integration. Nature Microbiology
Shipman SL, Nivala J, Macklis JD, et al. (2017) CRISPR-Cas encoding of a digital movie into the genomes of a population of living bacteria. Nature
Stranges PB, Palla M, Kalachikov S, et al. (2016) Design and characterization of a nanopore-coupled polymerase for single-molecule DNA sequencing by synthesis on an electrode array. Proceedings of the National Academy of Sciences of the United States of America. 113: E6749-E6756
Shipman SL, Nivala J, Macklis JD, et al. (2016) Molecular recordings by directed CRISPR spacer acquisition. Science (New York, N.Y.). 353: aaf1175
Nivala J, Mulroney L, Li G, et al. (2014) Discrimination among protein variants using an unfoldase-coupled nanopore. Acs Nano. 8: 12365-75
Nivala J, Marks DB, Akeson M. (2013) Unfoldase-mediated protein translocation through an α-hemolysin nanopore. Nature Biotechnology. 31: 247-50
Wu SJ, Eiben CB, Carra JH, et al. (2011) Improvement of a potential anthrax therapeutic by computational protein design. The Journal of Biological Chemistry. 286: 32586-92
Actis P, Rogers A, Nivala J, et al. (2011) Reversible thrombin detection by aptamer functionalized STING sensors. Biosensors & Bioelectronics. 26: 4503-7
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