Alan A. Doucette - Publications

Affiliations: 
2002 University of Alberta, Edmonton, Alberta, Canada 

46 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2024 Nickerson JL, Gagnon H, Wentzell PD, Doucette AA. Assessing the precision of a detergent-assisted cartridge precipitation workflow for non-targeted quantitative proteomics. Proteomics. e2300339. PMID 38299459 DOI: 10.1002/pmic.202300339  0.549
2022 Nickerson JL, Doucette AA. Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up Proteome Analysis. Biology. 11. PMID 36290348 DOI: 10.3390/biology11101444  0.408
2022 Nickerson JL, Baghalabadi V, Dang Z, Miller VA, Little SL, Doucette AA. Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry. Journal of Visualized Experiments : Jove. PMID 35188123 DOI: 10.3791/63503  0.567
2021 Baghalabadi V, Razmi H, Doucette A. Salt-Mediated Organic Solvent Precipitation for Enhanced Recovery of Peptides Generated by Pepsin Digestion. Proteomes. 9. PMID 34842819 DOI: 10.3390/proteomes9040044  0.472
2021 Jakubec PJ, Doucette AA. Automated Electrokinetic Platform for High-Throughput Sodium Dodecyl Sulfate Depletion Ahead of Proteome Analysis by Mass Spectrometry. Analytical Chemistry. 93: 14042-14047. PMID 34644045 DOI: 10.1021/acs.analchem.1c03549  0.472
2021 Nickerson JL, Baghalabadi V, Rajendran SRCK, Jakubec PJ, Said H, McMillen TS, Dang Z, Doucette AA. Recent advances in top-down proteome sample processing ahead of MS analysis. Mass Spectrometry Reviews. PMID 34047392 DOI: 10.1002/mas.21706  0.518
2020 Baghalabadi V, Doucette AA. Mass spectrometry profiling of low molecular weight proteins and peptides isolated by acetone precipitation. Analytica Chimica Acta. 1138: 38-48. PMID 33161983 DOI: 10.1016/j.aca.2020.08.057  0.52
2020 Nickerson JL, Doucette AA. Rapid and quantitative protein precipitation for proteome analysis by mass spectrometry. Journal of Proteome Research. PMID 32195589 DOI: 10.1021/acs.jproteome.9b00867  0.541
2018 Unterlander N, Doucette AA. Membrane-Based SDS Depletion Ahead of Peptide and Protein Analysis by Mass Spectrometry. Proteomics. e1700025. PMID 29575800 DOI: 10.1002/pmic.201700025  0.526
2018 Unterlander N, Doucette AA. Accelerated SDS depletion from proteins by transmembrane electrophoresis: Impacts of Joule heating. Electrophoresis. 39: 1349-1356. PMID 29417596 DOI: 10.1002/elps.201700410  0.502
2018 Orton DJ, Doucette AA, Huang W, MacLellan DL. Exosomal proteomic analysis reveals changes in the urinary proteome of rats with unilateral ureteral obstruction Canadian Journal of Chemistry. 96: 771-778. DOI: 10.1139/CJC-2017-0681  0.355
2017 Griffiths SG, Cormier MT, Clayton A, Doucette AA. Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by Chaperone Affinity Enrichment. Proteomes. 5. PMID 28991197 DOI: 10.3390/proteomes5040025  0.309
2017 Kachuk C, Doucette AA. The benefits (and misfortunes) of SDS in top-down proteomics. Journal of Proteomics. PMID 28286130 DOI: 10.1016/j.jprot.2017.03.002  0.562
2017 Güray MZ, Zheng S, Doucette AA. Mass spectrometry of intact proteins reveals +98 u chemical artefacts following precipitation in acetone. Journal of Proteome Research. PMID 28088865 DOI: 10.1021/acs.jproteome.6b00841  0.535
2016 Kachuk C, Faulkner M, Liu F, Doucette AA. Automated SDS depletion for mass spectrometry of intact membrane proteins though transmembrane electrophoresis. Journal of Proteome Research. PMID 27376408 DOI: 10.1021/acs.jproteome.6b00199  0.568
2015 Kachuk C, Stephen K, Doucette A. Comparison of sodium dodecyl sulfate depletion techniques for proteome analysis by mass spectrometry. Journal of Chromatography. A. 1418: 158-66. PMID 26422304 DOI: 10.1016/j.chroma.2015.09.042  0.586
2015 Crowell AM, MacLellan DL, Doucette AA. A two-stage spin cartridge for integrated protein precipitation, digestion and SDS removal in a comparative bottom-up proteomics workflow. Journal of Proteomics. 118: 140-50. PMID 25316050 DOI: 10.1016/j.jprot.2014.09.030  0.474
2014 Doucette AA, Vieira DB, Orton DJ, Wall MJ. Resolubilization of precipitated intact membrane proteins with cold formic acid for analysis by mass spectrometry. Journal of Proteome Research. 13: 6001-12. PMID 25384094 DOI: 10.1021/pr500864a  0.485
2014 Orton DJ, Arsenault DJ, Thomas NA, Doucette AA. Reprint of "GELFrEE fractionation combined with mass spectrometry for proteome analysis of secreted toxins from Enteropathogenic Escherichia coli (EPEC)". Molecular and Cellular Probes. 28: 83-90. PMID 24486296 DOI: 10.1016/j.mcp.2014.01.004  0.468
2014 Orton DJ, Doucette AA, Maksym GN, MacLellan DL. Proteomic analysis of rat proximal tubule cells following stretch-induced apoptosis in an in vitro model of kidney obstruction. Journal of Proteomics. 100: 125-35. PMID 24316357 DOI: 10.1016/j.jprot.2013.11.025  0.416
2013 Orton DJ, Doucette AA. Proteomic Workflows for Biomarker Identification Using Mass Spectrometry - Technical and Statistical Considerations during Initial Discovery. Proteomes. 1: 109-127. PMID 28250400 DOI: 10.3390/proteomes1020109  0.442
2013 Orton DJ, Wall MJ, Doucette AA. Dual LC-MS platform for high-throughput proteome analysis. Journal of Proteome Research. 12: 5963-70. PMID 24090060 DOI: 10.1021/pr400738a  0.529
2013 Crowell AM, Wall MJ, Doucette AA. Maximizing recovery of water-soluble proteins through acetone precipitation. Analytica Chimica Acta. 796: 48-54. PMID 24016582 DOI: 10.1016/j.aca.2013.08.005  0.452
2013 Orton DJ, Arsenault DJ, Thomas NA, Doucette AA. GELFrEE fractionation combined with mass spectrometry for proteome analysis of secreted toxins from Enteropathogenic Escherichia coli (EPEC). Molecular and Cellular Probes. 27: 200-7. PMID 23831145 DOI: 10.1016/j.mcp.2013.06.004  0.468
2013 Orton DJ, Doucette AA. A universal, high recovery assay for protein quantitation through temperature programmed liquid chromatography (TPLC). Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 921: 75-80. PMID 23435344 DOI: 10.1016/j.jchromb.2013.01.021  0.438
2013 Crowell AM, Stewart EJ, Take ZS, Doucette AA. Critical assessment of the spectroscopic activity assay for monitoring trypsin activity in organic-aqueous solvent. Analytical Biochemistry. 435: 131-6. PMID 23333224 DOI: 10.1016/j.ab.2012.12.019  0.322
2012 Boutilier JM, Warden H, Doucette AA, Wentzell PD. Chromatographic behaviour of peptides following dimethylation with H2/D2-formaldehyde: implications for comparative proteomics. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 908: 59-66. PMID 23122402 DOI: 10.1016/J.Jchromb.2012.09.035  0.311
2012 Vieira DB, Crowell AM, Doucette AA. Perfluorooctanoic acid and ammonium perfluorooctanoate: volatile surfactants for proteome analysis? Rapid Communications in Mass Spectrometry : Rcm. 26: 523-31. PMID 22302492 DOI: 10.1002/rcm.6127  0.427
2011 Doucette AA, Tran JC, Wall MJ, Fitzsimmons S. Intact proteome fractionation strategies compatible with mass spectrometry. Expert Review of Proteomics. 8: 787-800. PMID 22087661 DOI: 10.1586/epr.11.67  0.615
2011 Wall MJ, Crowell AM, Simms GA, Carey GH, Liu F, Doucette AA. Implications of partial tryptic digestion in organic-aqueous solvent systems for bottom-up proteome analysis. Analytica Chimica Acta. 703: 194-203. PMID 21889634 DOI: 10.1016/j.aca.2011.07.025  0.521
2010 Botelho D, Wall MJ, Vieira DB, Fitzsimmons S, Liu F, Doucette A. Top-down and bottom-up proteomics of SDS-containing solutions following mass-based separation. Journal of Proteome Research. 9: 2863-70. PMID 20377267 DOI: 10.1021/pr900949p  0.564
2009 Mataija-Botelho D, Murphy P, Pinto DM, Maclellan DL, Langlois C, Doucette AA. A qualitative proteome investigation of the sediment portion of human urine: Implications in the biomarker discovery process. Proteomics. Clinical Applications. 3: 95-105. PMID 21136939 DOI: 10.1002/prca.200800019  0.399
2009 Tran JC, Doucette AA. Multiplexed size separation of intact proteins in solution phase for mass spectrometry. Analytical Chemistry. 81: 6201-9. PMID 19572727 DOI: 10.1021/ac900729r  0.452
2009 Tran JC, Wall MJ, Doucette AA. Evaluation of a solution isoelectric focusing protocol as an alternative to ion exchange chromatography for charge-based proteome prefractionation. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. 877: 807-13. PMID 19246255 DOI: 10.1016/j.jchromb.2009.02.024  0.488
2008 Tran JC, Doucette AA. Rapid and effective focusing in a carrier ampholyte solution isoelectric focusing system: a proteome prefractionation tool. Journal of Proteome Research. 7: 1761-6. PMID 18284188 DOI: 10.1021/pr700677u  0.481
2008 Tran JC, Doucette AA. Gel-eluted liquid fraction entrapment electrophoresis: an electrophoretic method for broad molecular weight range proteome separation. Analytical Chemistry. 80: 1568-73. PMID 18229945 DOI: 10.1021/ac702197w  0.556
2006 Tran JC, Doucette AA. Cyclic polyamide oligomers extracted from nylon 66 membrane filter disks as a source of contamination in liquid chromatography/mass spectrometry. Journal of the American Society For Mass Spectrometry. 17: 652-6. PMID 16517177 DOI: 10.1016/j.jasms.2006.01.008  0.304
2003 Doucette A, Craft D, Li L. Mass spectrometric study of the effects of hydrophobic surface chemistry and morphology on the digestion of surface-bound proteins Journal of the American Society For Mass Spectrometry. 14: 203-214. PMID 12648927 DOI: 10.1016/S1044-0305(02)00909-1  0.626
2002 Craft D, Doucette A, Li L. Microcolumn capture and digestion of proteins combined with mass spectrometry for protein identification Journal of Proteome Research. 1: 537-547. PMID 12645622 DOI: 10.1021/Pr0255598  0.686
2002 Nesatyy VJ, Doucette A, Ross NW. Mass spectrometry analysis of intact proteins recovered from silver stained gels Proceedings 50th Asms Conference On Mass Spectrometry and Allied Topics. 155-156.  0.549
2002 Craft DR, Doucette AA, Li L. An automated approach to in-column digestion for MS protein identification Proceedings 50th Asms Conference On Mass Spectrometry and Allied Topics. 513-514.  0.643
2002 Doucette AA, Stupak J, Zhang N, Li L. Application of a dual enzyme digestion procedure for protein identification by MALDI MS/MS in a Q-TOF instrument Proceedings 50th Asms Conference On Mass Spectrometry and Allied Topics. 565-566.  0.61
2001 Doucette A, Li L. Investigation of the applicability of a sequential digestion protocol using trypsin and leucine aminopeptidase M for protein identification by matrix-assisted laser desorption/ionization - Time of flight mass spectrometry Proteomics. 1: 987-1000. PMID 11683515 DOI: 10.1002/1615-9861(200108)1:8<987::Aid-Prot987>3.0.Co;2-5  0.639
2001 Zhang N, Doucette A, Li L. Two-layer sample preparation method for MALDI mass spectrometric analysis of protein and peptide samples containing sodium dodecyl sulfate Analytical Chemistry. 73: 2968-2975. PMID 11467542 DOI: 10.1021/Ac001418I  0.596
2001 Doucette A, Li L. Investigation of the applicability of a sequential digestion protocol using trypsin and leucine aminopeptidase M for protein identification by matrix-assisted laser desorption/ionization mass spectrometry European Journal of Mass Spectrometry. 7: 157-170.  0.577
2000 Doucette A, Craft D, Li L. Protein concentration and enzyme digestion on microbeads for MALDI-TOF peptide mass mapping of proteins from dilute solutions Analytical Chemistry. 72: 3355-3362. PMID 10939411 DOI: 10.1021/Ac000176J  0.651
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