Karen M. Polizzi, Ph.D. - Publications

2005 Georgia Institute of Technology, Atlanta, GA 
Chemical Engineering

50 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2022 Spice AJ, Aw R, Polizzi KM. Cell-Free Protein Synthesis Using Pichia pastoris. Methods in Molecular Biology (Clifton, N.J.). 2433: 75-88. PMID 34985738 DOI: 10.1007/978-1-0716-1998-8_4  0.309
2020 Spice AJ, Aw R, Bracewell DG, Polizzi KM. Improving the reaction mix of a cell-free system using a design of experiments approach to minimise experimental effort. Synthetic and Systems Biotechnology. 5: 137-144. PMID 32637667 DOI: 10.1016/J.Synbio.2020.06.003  0.397
2020 Arpino JAJ, Polizzi KM. A Modular Method for Directing Protein Self-Assembly. Acs Synthetic Biology. 9: 993-1002. PMID 32243747 DOI: 10.1021/Acssynbio.9B00504  0.372
2020 Spice AJ, Aw R, Bracewell DG, Polizzi KM. Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Cell-Free System. Frontiers in Bioengineering and Biotechnology. 8: 72. PMID 32117947 DOI: 10.3389/Fbioe.2020.00072  0.346
2019 Riangrungroj P, Bever CS, Hammock BD, Polizzi KM. A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure. Scientific Reports. 9: 12466. PMID 31462650 DOI: 10.1038/S41598-019-48907-6  0.308
2019 Riangrungroj P, Polizzi KM. BeQuIK (Biosensor Engineered Quorum Induced Killing): designer bacteria for destroying recalcitrant biofilms. Microbial Biotechnology. PMID 31328393 DOI: 10.1111/1751-7915.13465  0.302
2019 Moya Ramírez I, Kontoravdi C, Polizzi KM. Low-cost and user-friendly biosensor to test the integrity of mRNA molecules suitable for field applications. Biosensors & Bioelectronics. 137: 199-206. PMID 31100599 DOI: 10.1016/J.Bios.2019.05.008  0.309
2019 Kylilis N, Riangrungroj P, Lai HE, Salema V, Fernández LÁ, Stan GB, Freemont PS, Polizzi KM. Whole-cell biosensor with tuneable limit of detection enables low-cost agglutination assays for medical diagnostic applications. Acs Sensors. PMID 30623662 DOI: 10.1021/Acssensors.8B01163  0.322
2019 Aw R, Polizzi KM. Biosensor-assisted engineering of a high-yield Pichia pastoris cell-free protein synthesis platform. Biotechnology and Bioengineering. 116: 656-666. PMID 30552674 DOI: 10.1002/Bit.26901  0.411
2018 Trantidou T, Dekker L, Polizzi K, Ces O, Elani Y. Functionalizing cell-mimetic giant vesicles with encapsulated bacterial biosensors. Interface Focus. 8: 20180024. PMID 30443325 DOI: 10.1098/Rsfs.2018.0024  0.325
2018 Jonas FRH, Royle KE, Aw R, Stan GV, Polizzi KM. Investigating the consequences of asymmetric endoplasmic reticulum inheritance in under stress using a combination of single cell measurements and mathematical modelling. Synthetic and Systems Biotechnology. 3: 64-75. PMID 29911200 DOI: 10.1016/J.Synbio.2018.01.001  0.316
2018 Lai HE, Moore S, Polizzi K, Freemont P. EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. Methods in Molecular Biology (Clifton, N.J.). 1772: 429-444. PMID 29754244 DOI: 10.1007/978-1-4939-7795-6_25  0.363
2018 Moore SJ, MacDonald JT, Wienecke S, Ishwarbhai A, Tsipa A, Aw R, Kylilis N, Bell DJ, McClymont DW, Jensen K, Polizzi KM, Biedendieck R, Freemont PS. Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria. Proceedings of the National Academy of Sciences of the United States of America. PMID 29666238 DOI: 10.1073/Pnas.1715806115  0.306
2017 Royle KE, Polizzi K. A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Scientific Reports. 7: 15817. PMID 29150665 DOI: 10.1038/S41598-017-16172-0  0.36
2017 Sou SN, Lee K, Nayyar K, Polizzi KM, Sellick C, Kontoravdi C. Exploring cellular behaviour under transient gene expression and its impact on mAb productivity and Fc-glycosylation. Biotechnology and Bioengineering. PMID 28921534 DOI: 10.1002/Bit.26456  0.372
2017 Dekker L, Polizzi KM. Sense and sensitivity in bioprocessing-detecting cellular metabolites with biosensors. Current Opinion in Chemical Biology. 40: 31-36. PMID 28609710 DOI: 10.1016/J.Cbpa.2017.05.014  0.317
2017 Walker BJ, Stan GV, Polizzi KM. Intracellular delivery of biologic therapeutics by bacterial secretion systems. Expert Reviews in Molecular Medicine. 19: e6. PMID 28382885 DOI: 10.1017/Erm.2017.7  0.328
2017 Moore SJ, Lai HE, Needham H, Polizzi KM, Freemont PS. Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool. Biotechnology Journal. PMID 28139884 DOI: 10.1002/Biot.201600678  0.403
2017 Goers L, Ainsworth C, Goey CH, Kontoravdi C, Freemont PS, Polizzi KM. Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production. Biotechnology and Bioengineering. PMID 28112405 DOI: 10.1002/Bit.26254  0.341
2017 .Ogonah OW, Polizzi KM, Bracewell DG. Cell free protein synthesis: a viable option for stratified medicines manufacturing? Current Opinion in Chemical Engineering. 18: 77-83. DOI: 10.1016/J.Coche.2017.10.003  0.362
2016 Sou SN, Jedrzejewski PM, Lee K, Sellick C, Polizzi KM, Kontoravdi C. Model-based investigation of intracellular processes determining antibody Fc-glycosylation under mild hypothermia. Biotechnology and Bioengineering. PMID 27869292 DOI: 10.1002/Bit.26225  0.339
2016 Del Val IJ, Polizzi KM, Kontoravdi C. A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation. Scientific Reports. 6: 28547. PMID 27345611 DOI: 10.1038/Srep28547  0.311
2016 Moore SJ, Lai HE, Kelwick R, Chee SM, Bell D, Polizzi KM, Freemont PS. EcoFlex - A Multifunctional MoClo Kit for E. coli Synthetic Biology. Acs Synthetic Biology. PMID 27096716 DOI: 10.1021/Acssynbio.6B00031  0.404
2016 Aw R, Polizzi KM. Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris. Microbial Cell Factories. 15: 29. PMID 26849882 DOI: 10.1186/S12934-016-0432-8  0.361
2016 Klymenko OV, Shah N, Kontoravdi C, Royle KE, Polizzi KM. Designing an Artificial Golgi reactor to achieve targeted glycosylation of monoclonal antibodies Aiche Journal. DOI: 10.1002/Aic.15388  0.335
2015 Sou SN, Sellick C, Lee K, Mason A, Kyriakopoulos S, Polizzi KM, Kontoravdi C. How does mild hypothermia affect monoclonal antibody glycosylation? Biotechnology and Bioengineering. 112: 1165-76. PMID 25545631 DOI: 10.1002/Bit.25524  0.334
2014 Jedrzejewski PM, del Val IJ, Constantinou A, Dell A, Haslam SM, Polizzi KM, Kontoravdi C. Towards controlling the glycoform: a model framework linking extracellular metabolites to antibody glycosylation. International Journal of Molecular Sciences. 15: 4492-522. PMID 24637934 DOI: 10.3390/Ijms15034492  0.313
2014 Behjousiar A, Constantinou A, Polizzi KM, Kontoravdi C. FIBS-enabled noninvasive metabolic profiling. Journal of Visualized Experiments : Jove. e51200. PMID 24513729 DOI: 10.3791/51200  0.344
2013 Aw R, Polizzi KM. Can too many copies spoil the broth? Microbial Cell Factories. 12: 128. PMID 24354594 DOI: 10.1186/1475-2859-12-128  0.405
2013 Constantinou A, Polizzi KM. Opportunities for bioprocess monitoring using FRET biosensors. Biochemical Society Transactions. 41: 1146-51. PMID 24059500 DOI: 10.1042/Bst20130103  0.352
2013 del Val IJ, Kyriakopoulos S, Polizzi KM, Kontoravdi C. An optimized method for extraction and quantification of nucleotides and nucleotide sugars from mammalian cells. Analytical Biochemistry. 443: 172-80. PMID 24036437 DOI: 10.1016/J.Ab.2013.09.005  0.317
2013 Arpino JA, Hancock EJ, Anderson J, Barahona M, Stan GB, Papachristodoulou A, Polizzi K. Tuning the dials of Synthetic Biology. Microbiology (Reading, England). 159: 1236-53. PMID 23704788 DOI: 10.1099/Mic.0.067975-0  0.335
2013 Sou SN, Polizzi KM, Kontoravdi C. Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells Advances in Bioscience and Biotechnology. 4: 1013-1019. DOI: 10.4236/Abb.2013.412135  0.328
2013 Jedrzejewski PMJ, del Val IJ, Polizzi KM, Kontoravdi C. Applying quality by design to glycoprotein therapeutics: experimental and computational efforts of process control Pharmaceutical Bioprocessing. 1: 51-69. DOI: 10.4155/Pbp.13.4  0.345
2013 Goers L, Kylilis N, Tomazou M, Wen KY, Freemont P, Polizzi K. Engineering Microbial Biosensors Methods in Microbiology. 40: 119-156. DOI: 10.1016/B978-0-12-417029-2.00005-4  0.321
2012 Behjousiar A, Kontoravdi C, Polizzi KM. In situ monitoring of intracellular glucose and glutamine in CHO cell culture. Plos One. 7: e34512. PMID 22509313 DOI: 10.1371/Journal.Pone.0034512  0.354
2012 Polizzi KM, Isaacson RL. Protein construct optimization: data sharing strategy. Protein & Cell. 3: 321-2. PMID 22410785 DOI: 10.1007/S13238-012-2022-9  0.318
2012 Chen N, Koumpouras GC, Polizzi KM, Kontoravdi C. Genome-based kinetic modeling of cytosolic glucose metabolism in industrially relevant cell lines: Saccharomyces cerevisiae and Chinese hamster ovary cells. Bioprocess and Biosystems Engineering. 35: 1023-33. PMID 22286123 DOI: 10.1007/S00449-012-0687-3  0.327
2010 Szita N, Polizzi K, Jaccard N, Baganz F. Microfluidic approaches for systems and synthetic biology. Current Opinion in Biotechnology. 21: 517-23. PMID 20829028 DOI: 10.1016/J.Copbio.2010.08.002  0.339
2007 Chaparro-Riggers JF, Loo BL, Polizzi KM, Gibbs PR, Tang XS, Nelson MJ, Bommarius AS. Revealing biases inherent in recombination protocols. Bmc Biotechnology. 7: 77. PMID 18001472 DOI: 10.1186/1472-6750-7-77  0.72
2007 Polizzi KM, Moore DA, Bommarius AS. A short-chain dehydrogenase/reductase from Vibrio vulnificus with both blue fluorescence and oxidoreductase activity. Chemical Communications (Cambridge, England). 1843-5. PMID 17476406 DOI: 10.1039/B616763B  0.566
2007 Polizzi KM, Bommarius AS, Broering JM, Chaparro-Riggers JF. Stability of biocatalysts. Current Opinion in Chemical Biology. 11: 220-5. PMID 17307381 DOI: 10.1016/J.Cbpa.2007.01.685  0.748
2007 Chaparro-Riggers JF, Polizzi KM, Bommarius AS. Better library design: data-driven protein engineering. Biotechnology Journal. 2: 180-91. PMID 17183506 DOI: 10.1002/Biot.200600170  0.631
2007 Chaparro-Riggers JF, Rogers TA, Vazquez-Figueroa E, Polizzi KM, Bommarius AS. Comparison of three enoate reductases and their potential use for biotransformations Advanced Synthesis and Catalysis. 349: 1521-1531. DOI: 10.1002/Adsc.200700074  0.706
2006 Bommarius AS, Broering JM, Chaparro-Riggers JF, Polizzi KM. High-throughput screening for enhanced protein stability. Current Opinion in Biotechnology. 17: 606-10. PMID 17049838 DOI: 10.1016/J.Copbio.2006.10.001  0.762
2006 Polizzi KM, Chaparro-Riggers JF, Vazquez-Figueroa E, Bommarius AS. Structure-guided consensus approach to create a more thermostable penicillin G acylase. Biotechnology Journal. 1: 531-6. PMID 16892288 DOI: 10.1002/Biot.200600029  0.711
2006 Polizzi KM, Parikh M, Spencer CU, Matsumura I, Lee JH, Realff MJ, Bommarius AS. Pooling for improved screening of combinatorial libraries for directed evolution. Biotechnology Progress. 22: 961-7. PMID 16889370 DOI: 10.1021/Bp060099Z  0.596
2006 Bommarus AS, Polizzi KM. Novel biocatalysts: Recent developments Chemical Engineering Science. 61: 1004-1016. DOI: 10.1016/J.Ces.2005.03.053  0.37
2005 Polizzi KM, Spencer CU, Dubey A, Matsumura I, Lee JH, Realff MJ, Bommarius AS. Simulation modeling of pooling for combinatorial protein engineering. Journal of Biomolecular Screening. 10: 856-64. PMID 16234344 DOI: 10.1177/1087057105280134  0.62
2005 Polizzi KM, Chaparro-Riggers J, Loo B, Luna A, Vazquez-Figueroa E, Bommarius AS. Increasing the synthetic utility of penicillin G acylase by rational and directed evolution Aiche Annual Meeting, Conference Proceedings. 9183.  0.739
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