David E. Cane, Ph.D. - Publications

Affiliations: 
1974- Chemistry Brown University, Providence, RI 
Area:
Chemistry, Enzymology and Molecular Genetics of Natural Products Biosynthesis
Website:
http://www.chem.brown.edu/Faculty/dec/dec.html

215 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2018 Xie X, Cane DE. pH-Rate profiles establish that polyketide synthase dehydratase domains utilize a single-base mechanism. Organic & Biomolecular Chemistry. PMID 30457629 DOI: 10.1039/c8ob02637h  1
2018 Li X, Sevillano N, La Greca F, Deis L, Liu YC, Deller MC, Mathews II, Matsui T, Cane DE, Craik CS, Khosla C. Structure-Function Analysis of the Extended Conformation of a Polyketide Synthase Module. Journal of the American Chemical Society. PMID 29762030 DOI: 10.1021/jacs.8b02100  0.76
2018 Xie X, Cane DE. Stereospecific Formation of Z-Trisubstituted Double Bonds by the Successive Action of Ketoreductase and Dehydratase Domains from trans-AT Polyketide Synthases. Biochemistry. PMID 29293329 DOI: 10.1021/acs.biochem.7b01253  1
2017 Blank PN, Harris GG, Chou WKW, Duan L, Cane DE, Christianson DW. Substitution of Aromatic Residues with Polar Residues in the Active Site Pocket of epi-Isozizaene Synthase Leads to the Generation of New Cyclic Sesquiterpenes. Biochemistry. PMID 28967743 DOI: 10.1021/acs.biochem.7b00895  0.64
2017 You YO, Shah DD, Cane DE. Stereospecific Formation of E- and Z- Disubstituted Double Bonds by Dehydratase Domains from Modules 1 and 2 of the Fostriecin Polyketide Synthase. Journal of the American Chemical Society. PMID 28902510 DOI: 10.1021/jacs.7b08896  0.96
2017 Xie X, Garg A, Khosla C, Cane DE. Elucidation of the Cryptic Methyl Group Epimerase Activity of Dehydratase Domains from Modular Polyketide Synthases Using a Tandem Modules Epimerase Assay. Journal of the American Chemical Society. PMID 28682630 DOI: 10.1021/jacs.7b05502  1
2017 Xie X, Khosla C, Cane DE. Elucidation of the Stereospecificity of C-Methyltransferases from trans-AT Polyketide Synthases. Journal of the American Chemical Society. PMID 28430424 DOI: 10.1021/jacs.7b02911  1
2017 Pemberton TA, Chen M, Harris GG, Chou WK, Duan L, Köksal M, Genshaft AS, Cane DE, Christianson DW. Exploring the Influence of Domain Architecture on the Catalytic Function of Diterpene Synthases. Biochemistry. PMID 28362483 DOI: 10.1021/acs.biochem.7b00137  0.64
2017 Chou WK, Gould CA, Cane DE. Incubation of 2-methylisoborneol synthase with the intermediate analog 2-methylneryl diphosphate. The Journal of Antibiotics. PMID 28246382 DOI: 10.1038/ja.2017.24  0.48
2017 Xie X, Garg A, Khosla C, Cane DE. Mechanism and Stereochemistry of Polyketide Chain Elongation and Methyl Group Epimerization in Polyether Biosynthesis. Journal of the American Chemical Society. PMID 28157306 DOI: 10.1021/jacs.7b00278  1
2016 Chen K, Wu S, Zhu L, Zhang C, Xiang W, Deng Z, Ikeda H, Cane DE, Zhu D. Substitution of a Single Amino Acid Reverses the Regiospecificity of the Baeyer-Villiger Monooxygenase PntE in the Biosynthesis of the Antibiotic Pentalenolactone. Biochemistry. 55: 6696-6704. PMID 27933799 DOI: 10.1021/acs.biochem.6b01040  0.8
2016 Li Z, Gao R, Hao Q, Zhao H, Cheng L, He F, Liu L, Liu X, Chou WK, Zhu H, Cane DE. The T296V Mutant of Amorpha-4,11-diene Synthase Is Defective in Allylic Diphosphate Isomerization but Retains the Ability To Cyclize the Intermediate (3R)-Nerolidyl Diphosphate to Amorpha-4,11-diene. Biochemistry. 55: 6599-6604. PMID 27933789 DOI: 10.1021/acs.biochem.6b01004  0.48
2016 Duan L, Jogl G, Cane DE. The Cytochrome P450-Catalyzed Oxidative Rearrangement in the Final Step of Pentalenolactone Biosynthesis: Substrate Structure Determines Mechanism. Journal of the American Chemical Society. PMID 27588339 DOI: 10.1021/jacs.6b08610  0.48
2016 Robbins T, Kapilivsky J, Cane DE, Khosla C. Roles of Conserved Active Site Residues in the Ketosynthase Domain of an Assembly Line Polyketide Synthase. Biochemistry. PMID 27441852 DOI: 10.1021/acs.biochem.6b00639  0.76
2016 Robbins T, Liu YC, Cane DE, Khosla C. Structure and mechanism of assembly line polyketide synthases. Current Opinion in Structural Biology. 41: 10-18. PMID 27266330 DOI: 10.1016/j.sbi.2016.05.009  0.76
2016 Klaus M, Ostrowski MP, Austerjost J, Robbins T, Lowry B, Cane DE, Khosla C. Protein Protein Interactions, not Substrate Recognition, Dominates the Turnover of Chimeric Assembly Line Polyketide Synthases. The Journal of Biological Chemistry. PMID 27246853 DOI: 10.1074/jbc.M116.730531  0.76
2016 Chen M, Chou WK, Al-Lami N, Faraldos JA, Allemann RK, Cane DE, Christianson DW. Probing the Role of Active Site Water in the Sesquiterpene Cyclization Reaction Catalyzed by Aristolochene Synthase. Biochemistry. PMID 27172425 DOI: 10.1021/acs.biochem.6b00343  1
2016 Ostrowski MP, Cane DE, Khosla C. Recognition of acyl carrier proteins by ketoreductases in assembly line polyketide synthases. The Journal of Antibiotics. PMID 27118242 DOI: 10.1038/ja.2016.41  0.76
2016 Lowry B, Li X, Robbins T, Cane DE, Khosla C. A Turnstile Mechanism for the Controlled Growth of Biosynthetic Intermediates on Assembly Line Polyketide Synthases. Acs Central Science. 2: 14-20. PMID 26878060 DOI: 10.1021/acscentsci.5b00321  0.76
2016 Xie X, Garg A, Keatinge-Clay AT, Khosla C, Cane DE. The Epimerase and Reductase Activities of Polyketide Synthase Ketoreductase Domains Utilize the Same Conserved Tyrosine and Serine Residues. Biochemistry. PMID 26863427 DOI: 10.1021/acs.biochem.6b00024  1
2016 Chen M, Chou WK, Toyomasu T, Cane DE, Christianson DW. Structure and Function of Fusicoccadiene Synthase, a Hexameric Bifunctional Diterpene Synthase. Acs Chemical Biology. PMID 26734760 DOI: 10.1021/acschembio.5b00960  0.64
2015 Harris GG, Lombardi PM, Pemberton TA, Matsui T, Weiss TM, Cole KE, Köksal M, Murphy FV, Vedula LS, Chou WK, Cane DE, Christianson DW. Structural Studies of Geosmin Synthase, a Bifunctional Sesquiterpene Synthase with αα Domain Architecture That Catalyzes a Unique Cyclization-Fragmentation Reaction Sequence. Biochemistry. PMID 26598179 DOI: 10.1021/acs.biochem.5b01143  1
2015 Yamada Y, Arima S, Nagamitsu T, Johmoto K, Uekusa H, Eguchi T, Shin-Ya K, Cane DE, Ikeda H. Novel terpenes generated by heterologous expression of bacterial terpene synthase genes in an engineered Streptomyces host. The Journal of Antibiotics. 68: 385-94. PMID 25605043 DOI: 10.1038/ja.2014.171  1
2015 Yamada Y, Kuzuyama T, Komatsu M, Shin-Ya K, Omura S, Cane DE, Ikeda H. Terpene synthases are widely distributed in bacteria. Proceedings of the National Academy of Sciences of the United States of America. 112: 857-62. PMID 25535391 DOI: 10.1073/pnas.1422108112  1
2014 Garg A, Xie X, Keatinge-Clay A, Khosla C, Cane DE. Elucidation of the cryptic epimerase activity of redox-inactive ketoreductase domains from modular polyketide synthases by tandem equilibrium isotope exchange. Journal of the American Chemical Society. 136: 10190-3. PMID 25004372 DOI: 10.1021/ja5056998  1
2014 Dunn BJ, Watts KR, Robbins T, Cane DE, Khosla C. Comparative analysis of the substrate specificity of trans- versus cis-acyltransferases of assembly line polyketide synthases. Biochemistry. 53: 3796-806. PMID 24871074 DOI: 10.1021/bi5004316  1
2014 Khosla C, Herschlag D, Cane DE, Walsh CT. Assembly line polyketide synthases: mechanistic insights and unsolved problems. Biochemistry. 53: 2875-83. PMID 24779441 DOI: 10.1021/bi500290t  1
2014 Lin HC, Tsunematsu Y, Dhingra S, Xu W, Fukutomi M, Chooi YH, Cane DE, Calvo AM, Watanabe K, Tang Y. Generation of complexity in fungal terpene biosynthesis: discovery of a multifunctional cytochrome P450 in the fumagillin pathway. Journal of the American Chemical Society. 136: 4426-36. PMID 24568283 DOI: 10.1021/ja500881e  1
2014 Li R, Chou WK, Himmelberger JA, Litwin KM, Harris GG, Cane DE, Christianson DW. Reprogramming the chemodiversity of terpenoid cyclization by remolding the active site contour of epi-isozizaene synthase. Biochemistry. 53: 1155-68. PMID 24517311 DOI: 10.1021/bi401643u  1
2013 Gay D, You YO, Keatinge-Clay A, Cane DE. Structure and stereospecificity of the dehydratase domain from the terminal module of the rifamycin polyketide synthase. Biochemistry. 52: 8916-28. PMID 24274103 DOI: 10.1021/bi400988t  1
2013 Garg A, Khosla C, Cane DE. Coupled methyl group epimerization and reduction by polyketide synthase ketoreductase domains. Ketoreductase-catalyzed equilibrium isotope exchange. Journal of the American Chemical Society. 135: 16324-7. PMID 24161343 DOI: 10.1021/ja408944s  1
2013 Lowry B, Robbins T, Weng CH, O'Brien RV, Cane DE, Khosla C. In vitro reconstitution and analysis of the 6-deoxyerythronolide B synthase. Journal of the American Chemical Society. 135: 16809-12. PMID 24161212 DOI: 10.1021/ja409048k  1
2013 Chen M, Al-lami N, Janvier M, D'Antonio EL, Faraldos JA, Cane DE, Allemann RK, Christianson DW. Mechanistic insights from the binding of substrate and carbocation intermediate analogues to aristolochene synthase. Biochemistry. 52: 5441-53. PMID 23905850 DOI: 10.1021/bi400691v  1
2013 Köksal M, Chou WK, Cane DE, Christianson DW. Unexpected reactivity of 2-fluorolinalyl diphosphate in the active site of crystalline 2-methylisoborneol synthase. Biochemistry. 52: 5247-55. PMID 23844678 DOI: 10.1021/bi400797c  1
2013 You YO, Khosla C, Cane DE. Stereochemistry of reductions catalyzed by methyl-epimerizing ketoreductase domains of polyketide synthases. Journal of the American Chemical Society. 135: 7406-9. PMID 23659177 DOI: 10.1021/ja4014776  1
2013 Komatsu M, Komatsu K, Koiwai H, Yamada Y, Kozone I, Izumikawa M, Hashimoto J, Takagi M, Omura S, Shin-ya K, Cane DE, Ikeda H. Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites. Acs Synthetic Biology. 2: 384-96. PMID 23654282 DOI: 10.1021/sb3001003  1
2013 Dunn BJ, Cane DE, Khosla C. Mechanism and specificity of an acyltransferase domain from a modular polyketide synthase. Biochemistry. 52: 1839-41. PMID 23452124 DOI: 10.1021/bi400185v  1
2013 Zhu D, Wang Y, Zhang M, Ikeda H, Deng Z, Cane DE. Product-mediated regulation of pentalenolactone biosynthesis in Streptomyces species by the MarR/SlyA family activators PenR and PntR. Journal of Bacteriology. 195: 1255-66. PMID 23316039 DOI: 10.1128/JB.02079-12  1
2012 Yamada Y, Cane DE, Ikeda H. Diversity and analysis of bacterial terpene synthases. Methods in Enzymology. 515: 123-62. PMID 22999173 DOI: 10.1016/B978-0-12-394290-6.00007-0  1
2012 Harvey CJ, Puglisi JD, Pande VS, Cane DE, Khosla C. Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket. Journal of the American Chemical Society. 134: 12259-65. PMID 22741553 DOI: 10.1021/ja304682q  1
2012 Zu L, Xu M, Lodewyk MW, Cane DE, Peters RJ, Tantillo DJ. Effect of isotopically sensitive branching on product distribution for pentalenene synthase: support for a mechanism predicted by quantum chemistry. Journal of the American Chemical Society. 134: 11369-71. PMID 22738258 DOI: 10.1021/ja3043245  1
2012 Yuzawa S, Kapur S, Cane DE, Khosla C. Role of a conserved arginine residue in linkers between the ketosynthase and acyltransferase domains of multimodular polyketide synthases. Biochemistry. 51: 3708-10. PMID 22509729 DOI: 10.1021/bi300399u  1
2012 Köksal M, Chou WK, Cane DE, Christianson DW. Structure of 2-methylisoborneol synthase from Streptomyces coelicolor and implications for the cyclization of a noncanonical C-methylated monoterpenoid substrate. Biochemistry. 51: 3011-20. PMID 22455514 DOI: 10.1021/bi201827a  1
2012 Köksal M, Chou WK, Cane DE, Christianson DW. Structure of geranyl diphosphate C-methyltransferase from Streptomyces coelicolor and implications for the mechanism of isoprenoid modification. Biochemistry. 51: 3003-10. PMID 22455498 DOI: 10.1021/bi300109c  1
2012 Cane DE. Biochemistry: favouring the unfavoured. Nature. 483: 285-6. PMID 22422260 DOI: 10.1038/483285a  1
2012 Kapur S, Lowry B, Yuzawa S, Kenthirapalan S, Chen AY, Cane DE, Khosla C. Reprogramming a module of the 6-deoxyerythronolide B synthase for iterative chain elongation. Proceedings of the National Academy of Sciences of the United States of America. 109: 4110-5. PMID 22371562 DOI: 10.1073/pnas.1118734109  1
2012 Guo X, Liu T, Deng Z, Cane DE. Essential role of the donor acyl carrier protein in stereoselective chain translocation to a fully reducing module of the nanchangmycin polyketide synthase. Biochemistry. 51: 879-87. PMID 22229794 DOI: 10.1021/bi201768v  1
2012 Cane DE, Ikeda H. Exploration and mining of the bacterial terpenome. Accounts of Chemical Research. 45: 463-72. PMID 22039990 DOI: 10.1021/ar200198d  1
2011 Chou WK, Ikeda H, Cane DE. Cloning and characterization of Pfl_1841, a 2-methylenebornane synthase in Pseudomonas fluorescens PfO-1. Tetrahedron. 67: 6627-6632. PMID 21804650 DOI: 10.1016/j.tet.2011.05.084  1
2011 Wong FT, Jin X, Mathews II, Cane DE, Khosla C. Structure and mechanism of the trans-acting acyltransferase from the disorazole synthase. Biochemistry. 50: 6539-48. PMID 21707057 DOI: 10.1021/bi200632j  1
2011 Charkoudian LK, Liu CW, Capone S, Kapur S, Cane DE, Togni A, Seebach D, Khosla C. Probing the interactions of an acyl carrier protein domain from the 6-deoxyerythronolide B synthase. Protein Science : a Publication of the Protein Society. 20: 1244-55. PMID 21563224 DOI: 10.1002/pro.652  1
2011 Takamatsu S, Lin X, Nara A, Komatsu M, Cane DE, Ikeda H. Characterization of a silent sesquiterpenoid biosynthetic pathway in Streptomyces avermitilis controlling epi-isozizaene albaflavenone biosynthesis and isolation of a new oxidized epi-isozizaene metabolite. Microbial Biotechnology. 4: 184-91. PMID 21342464 DOI: 10.1111/j.1751-7915.2010.00209.x  1
2011 Zhu D, Seo MJ, Ikeda H, Cane DE. Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone. Journal of the American Chemical Society. 133: 2128-31. PMID 21284395 DOI: 10.1021/ja111279h  1
2011 Hu Y, Chou WK, Hopson R, Cane DE. Genome mining in Streptomyces clavuligerus: expression and biochemical characterization of two new cryptic sesquiterpene synthases. Chemistry & Biology. 18: 32-7. PMID 21276937 DOI: 10.1016/j.chembiol.2010.11.008  1
2011 Seo MJ, Zhu D, Endo S, Ikeda H, Cane DE. Genome mining in Streptomyces. Elucidation of the role of Baeyer-Villiger monooxygenases and non-heme iron-dependent dehydrogenase/oxygenases in the final steps of the biosynthesis of pentalenolactone and neopentalenolactone. Biochemistry. 50: 1739-54. PMID 21250661 DOI: 10.1021/bi1019786  1
2011 Doud EH, Perlstein DL, Wolpert M, Cane DE, Walker S. Two distinct mechanisms for TIM barrel prenyltransferases in bacteria. Journal of the American Chemical Society. 133: 1270-3. PMID 21214173 DOI: 10.1021/ja109578b  1
2011 Giglio S, Chou WK, Ikeda H, Cane DE, Monis PT. Biosynthesis of 2-methylisoborneol in cyanobacteria. Environmental Science & Technology. 45: 992-8. PMID 21174459 DOI: 10.1021/es102992p  1
2011 Lee HY, Harvey CJ, Cane DE, Khosla C. Improved precursor-directed biosynthesis in E. coli via directed evolution. The Journal of Antibiotics. 64: 59-64. PMID 21081955 DOI: 10.1038/ja.2010.129  1
2011 Takamatsu S, Xu LH, Fushinobu S, Shoun H, Komatsu M, Cane DE, Ikeda H. Pentalenic acid is a shunt metabolite in the biosynthesis of the pentalenolactone family of metabolites: hydroxylation of 1-deoxypentalenic acid mediated by CYP105D7 (SAV_7469) of Streptomyces avermitilis. The Journal of Antibiotics. 64: 65-71. PMID 21081950 DOI: 10.1038/ja.2010.135  1
2010 Kapur S, Chen AY, Cane DE, Khosla C. Molecular recognition between ketosynthase and acyl carrier protein domains of the 6-deoxyerythronolide B synthase. Proceedings of the National Academy of Sciences of the United States of America. 107: 22066-71. PMID 21127271 DOI: 10.1073/pnas.1014081107  1
2010 Valenzano CR, You YO, Garg A, Keatinge-Clay A, Khosla C, Cane DE. Stereospecificity of the dehydratase domain of the erythromycin polyketide synthase. Journal of the American Chemical Society. 132: 14697-9. PMID 20925342 DOI: 10.1021/ja107344h  1
2010 Guo X, Liu T, Valenzano CR, Deng Z, Cane DE. Mechanism and stereospecificity of a fully saturating polyketide synthase module: nanchangmycin synthase module 2 and its dehydratase domain. Journal of the American Chemical Society. 132: 14694-6. PMID 20925339 DOI: 10.1021/ja1073432  1
2010 Chou WK, Fanizza I, Uchiyama T, Komatsu M, Ikeda H, Cane DE. Genome mining in Streptomyces avermitilis: cloning and characterization of SAV_76, the synthase for a new sesquiterpene, avermitilol. Journal of the American Chemical Society. 132: 8850-1. PMID 20536237 DOI: 10.1021/ja103087w  1
2010 Cane DE. Programming of erythromycin biosynthesis by a modular polyketide synthase. The Journal of Biological Chemistry. 285: 27517-23. PMID 20522551 DOI: 10.1074/jbc.R110.144618  1
2010 Komatsu M, Uchiyama T, Omura S, Cane DE, Ikeda H. Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proceedings of the National Academy of Sciences of the United States of America. 107: 2646-51. PMID 20133795 DOI: 10.1073/pnas.0914833107  1
2010 Aaron JA, Lin X, Cane DE, Christianson DW. Structure of epi-isozizaene synthase from Streptomyces coelicolor A3(2), a platform for new terpenoid cyclization templates. Biochemistry. 49: 1787-97. PMID 20131801 DOI: 10.1021/bi902088z  1
2010 Wong FT, Chen AY, Cane DE, Khosla C. Protein-protein recognition between acyltransferases and acyl carrier proteins in multimodular polyketide synthases. Biochemistry. 49: 95-102. PMID 19921859 DOI: 10.1021/bi901826g  1
2009 Valenzano CR, Lawson RJ, Chen AY, Khosla C, Cane DE. The biochemical basis for stereochemical control in polyketide biosynthesis. Journal of the American Chemical Society. 131: 18501-11. PMID 19928853 DOI: 10.1021/ja908296m  1
2009 Zhao B, Lei L, Vassylyev DG, Lin X, Cane DE, Kelly SL, Yuan H, Lamb DC, Waterman MR. Crystal structure of albaflavenone monooxygenase containing a moonlighting terpene synthase active site. The Journal of Biological Chemistry. 284: 36711-9. PMID 19858213 DOI: 10.1074/jbc.M109.064683  1
2009 Jiang J, Tetzlaff CN, Takamatsu S, Iwatsuki M, Komatsu M, Ikeda H, Cane DE. Genome mining in Streptomyces avermitilis: A biochemical Baeyer-Villiger reaction and discovery of a new branch of the pentalenolactone family tree. Biochemistry. 48: 6431-40. PMID 19485417 DOI: 10.1021/bi900766w  1
2009 Wang CM, Hopson R, Lin X, Cane DE. Biosynthesis of the sesquiterpene botrydial in Botrytis cinerea. Mechanism and stereochemistry of the enzymatic formation of presilphiperfolan-8beta-ol. Journal of the American Chemical Society. 131: 8360-1. PMID 19476353 DOI: 10.1021/ja9021649  1
2009 Lin X, Cane DE. Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor. Mechanism and stereochemistry of the enzymatic formation of epi-isozizaene. Journal of the American Chemical Society. 131: 6332-3. PMID 19385616 DOI: 10.1021/ja901313v  1
2009 Liu T, Cane DE, Deng Z. The enzymology of polyether biosynthesis. Methods in Enzymology. 459: 187-214. PMID 19362641 DOI: 10.1016/S0076-6879(09)04609-6  1
2009 Khosla C, Kapur S, Cane DE. Revisiting the modularity of modular polyketide synthases. Current Opinion in Chemical Biology. 13: 135-43. PMID 19217343 DOI: 10.1016/j.cbpa.2008.12.018  1
2008 Pinedo C, Wang CM, Pradier JM, Dalmais B, Choquer M, Le Pêcheur P, Morgant G, Collado IG, Cane DE, Viaud M. Sesquiterpene synthase from the botrydial biosynthetic gene cluster of the phytopathogen Botrytis cinerea. Acs Chemical Biology. 3: 791-801. PMID 19035644 DOI: 10.1021/cb800225v  1
2008 Giglio S, Jiang J, Saint CP, Cane DE, Monis PT. Isolation and characterization of the gene associated with geosmin production in cyanobacteria. Environmental Science & Technology. 42: 8027-32. PMID 19031897 DOI: 10.1021/es801465w  1
2008 Castonguay R, Valenzano CR, Chen AY, Keatinge-Clay A, Khosla C, Cane DE. Stereospecificity of ketoreductase domains 1 and 2 of the tylactone modular polyketide synthase. Journal of the American Chemical Society. 130: 11598-9. PMID 18693734 DOI: 10.1021/ja804453p  1
2008 Wang CM, Cane DE. Biochemistry and molecular genetics of the biosynthesis of the earthy odorant methylisoborneol in Streptomyces coelicolor. Journal of the American Chemical Society. 130: 8908-9. PMID 18563898 DOI: 10.1021/ja803639g  1
2008 Liu T, Lin X, Zhou X, Deng Z, Cane DE. Mechanism of thioesterase-catalyzed chain release in the biosynthesis of the polyether antibiotic nanchangmycin. Chemistry & Biology. 15: 449-58. PMID 18482697 DOI: 10.1016/j.chembiol.2008.04.006  1
2008 Shishova EY, Yu F, Miller DJ, Faraldos JA, Zhao Y, Coates RM, Allemann RK, Cane DE, Christianson DW. X-ray crystallographic studies of substrate binding to aristolochene synthase suggest a metal ion binding sequence for catalysis. The Journal of Biological Chemistry. 283: 15431-9. PMID 18385128 DOI: 10.1074/jbc.M800659200  1
2008 Kapur S, Worthington A, Tang Y, Cane DE, Burkart MD, Khosla C. Mechanism based protein crosslinking of domains from the 6-deoxyerythronolide B synthase. Bioorganic & Medicinal Chemistry Letters. 18: 3034-8. PMID 18243693 DOI: 10.1016/j.bmcl.2008.01.073  1
2008 Zhao B, Lin X, Lei L, Lamb DC, Kelly SL, Waterman MR, Cane DE. Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2). The Journal of Biological Chemistry. 283: 8183-9. PMID 18234666 DOI: 10.1074/jbc.M710421200  1
2008 Jiang J, Cane DE. Geosmin biosynthesis. Mechanism of the fragmentation-rearrangement in the conversion of germacradienol to geosmin. Journal of the American Chemical Society. 130: 428-9. PMID 18095692 DOI: 10.1021/ja077792i  1
2008 Nawrath T, Dickschat JS, Müller R, Jiang J, Cane DE, Schulz S. Identification of (8S,9S,10S)-8,10-dimethyl-1-octalin, a key intermediate in the biosynthesis of geosmin in bacteria. Journal of the American Chemical Society. 130: 430-1. PMID 18095691 DOI: 10.1021/ja077790y  1
2008 Vedula LS, Jiang J, Zakharian T, Cane DE, Christianson DW. Structural and mechanistic analysis of trichodiene synthase using site-directed mutagenesis: probing the catalytic function of tyrosine-295 and the asparagine-225/serine-229/glutamate-233-Mg2+B motif. Archives of Biochemistry and Biophysics. 469: 184-94. PMID 17996718 DOI: 10.1016/j.abb.2007.10.015  1
2007 You Z, Omura S, Ikeda H, Cane DE, Jogl G. Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis. The Journal of Biological Chemistry. 282: 36552-60. PMID 17942405 DOI: 10.1074/jbc.M706358200  1
2007 Castonguay R, He W, Chen AY, Khosla C, Cane DE. Stereospecificity of ketoreductase domains of the 6-deoxyerythronolide B synthase. Journal of the American Chemical Society. 129: 13758-69. PMID 17918944 DOI: 10.1021/ja0753290  1
2007 Alekseyev VY, Liu CW, Cane DE, Puglisi JD, Khosla C. Solution structure and proposed domain domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase. Protein Science : a Publication of the Protein Society. 16: 2093-107. PMID 17893358 DOI: 10.1110/ps.073011407  1
2007 Jiang J, He X, Cane DE. Biosynthesis of the earthy odorant geosmin by a bifunctional Streptomyces coelicolor enzyme. Nature Chemical Biology. 3: 711-5. PMID 17873868 DOI: 10.1038/nchembio.2007.29  1
2007 Tang Y, Chen AY, Kim CY, Cane DE, Khosla C. Structural and mechanistic analysis of protein interactions in module 3 of the 6-deoxyerythronolide B synthase. Chemistry & Biology. 14: 931-43. PMID 17719492 DOI: 10.1016/j.chembiol.2007.07.012  1
2007 Vedula LS, Zhao Y, Coates RM, Koyama T, Cane DE, Christianson DW. Exploring biosynthetic diversity with trichodiene synthase. Archives of Biochemistry and Biophysics. 466: 260-6. PMID 17678871 DOI: 10.1016/j.abb.2007.06.016  1
2007 Chen AY, Cane DE, Khosla C. Structure-based dissociation of a type I polyketide synthase module. Chemistry & Biology. 14: 784-92. PMID 17656315 DOI: 10.1016/j.chembiol.2007.05.015  1
2007 Khosla C, Tang Y, Chen AY, Schnarr NA, Cane DE. Structure and mechanism of the 6-deoxyerythronolide B synthase. Annual Review of Biochemistry. 76: 195-221. PMID 17328673 DOI: 10.1146/annurev.biochem.76.053105.093515  1
2007 Shishova EY, Di Costanzo L, Cane DE, Christianson DW. X-ray crystal structure of aristolochene synthase from Aspergillus terreus and evolution of templates for the cyclization of farnesyl diphosphate. Biochemistry. 46: 1941-51. PMID 17261032 DOI: 10.1021/bi0622524  1
2007 You Z, Omura S, Ikeda H, Cane DE. Pentalenolactone biosynthesis: Molecular cloning and assignment of biochemical function to PtlF, a short-chain dehydrogenase from Streptomyces avermitilis, and identification of a new biosynthetic intermediate. Archives of Biochemistry and Biophysics. 459: 233-40. PMID 17178094 DOI: 10.1016/j.abb.2006.11.016  1
2006 Cane DE, He X, Kobayashi S, Omura S, Ikeda H. Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression, and mechanistic study of the germacradienol/geosmin synthase. The Journal of Antibiotics. 59: 471-9. PMID 17080683 DOI: 10.1038/ja.2006.66  1
2006 Quaderer R, Omura S, Ikeda H, Cane DE. Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis. Journal of the American Chemical Society. 128: 13036-7. PMID 17017767 DOI: 10.1021/ja0639214  1
2006 Liu T, You D, Valenzano C, Sun Y, Li J, Yu Q, Zhou X, Cane DE, Deng Z. Identification of NanE as the thioesterase for polyether chain release in nanchangmycin biosynthesis. Chemistry & Biology. 13: 945-55. PMID 16984884 DOI: 10.1016/j.chembiol.2006.07.006  1
2006 Tang Y, Kim CY, Mathews II, Cane DE, Khosla C. The 2.7-Angstrom crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase. Proceedings of the National Academy of Sciences of the United States of America. 103: 11124-9. PMID 16844787 DOI: 10.1073/pnas.0601924103  1
2006 Jiang J, He X, Cane DE. Geosmin biosynthesis. Streptomyces coelicolor germacradienol/germacrene D synthase converts farnesyl diphosphate to geosmin. Journal of the American Chemical Society. 128: 8128-9. PMID 16787064 DOI: 10.1021/ja062669x  1
2006 You Z, Omura S, Ikeda H, Cane DE. Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis. Journal of the American Chemical Society. 128: 6566-7. PMID 16704250 DOI: 10.1021/ja061469i  1
2006 Tetzlaff CN, You Z, Cane DE, Takamatsu S, Omura S, Ikeda H. A gene cluster for biosynthesis of the sesquiterpenoid antibiotic pentalenolactone in Streptomyces avermitilis. Biochemistry. 45: 6179-86. PMID 16681390 DOI: 10.1021/bi060419n  1
2006 Lin X, Hopson R, Cane DE. Genome mining in Streptomyces coelicolor: molecular cloning and characterization of a new sesquiterpene synthase. Journal of the American Chemical Society. 128: 6022-3. PMID 16669656 DOI: 10.1021/ja061292s  1
2006 Cane DE. How to evolve a silk purse from a sow's ear. Nature Chemical Biology. 2: 179-80. PMID 16547477 DOI: 10.1038/nchembio0406-179  1
2006 Chen AY, Schnarr NA, Kim CY, Cane DE, Khosla C. Extender unit and acyl carrier protein specificity of ketosynthase domains of the 6-deoxyerythronolide B synthase. Journal of the American Chemical Society. 128: 3067-74. PMID 16506788 DOI: 10.1021/ja058093d  1
2006 Cane DE. Brushes with sage. Archives of Biochemistry and Biophysics. 448: 117-22. PMID 16259939 DOI: 10.1016/j.abb.2005.08.023  1
2006 He W, Wu J, Khosla C, Cane DE. Macrolactonization to 10-deoxymethynolide catalyzed by the recombinant thioesterase of the picromycin/methymycin polyketide synthase. Bioorganic & Medicinal Chemistry Letters. 16: 391-4. PMID 16249083 DOI: 10.1016/j.bmcl.2005.09.077  1
2006 Moffet DA, Khosla C, Cane DE. Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2. Bioorganic & Medicinal Chemistry Letters. 16: 213-6. PMID 16213712 DOI: 10.1016/j.bmcl.2005.09.017  1
2006 Picaud S, Mercke P, He X, Sterner O, Brodelius M, Cane DE, Brodelius PE. Amorpha-4,11-diene synthase: mechanism and stereochemistry of the enzymatic cyclization of farnesyl diphosphate. Archives of Biochemistry and Biophysics. 448: 150-5. PMID 16143293 DOI: 10.1016/j.abb.2005.07.015  1
2005 Wu J, Zaleski TJ, Valenzano C, Khosla C, Cane DE. Polyketide double bond biosynthesis. Mechanistic analysis of the dehydratase-containing module 2 of the picromycin/methymycin polyketide synthase. Journal of the American Chemical Society. 127: 17393-404. PMID 16332089 DOI: 10.1021/ja055672+  1
2005 Wu J, He W, Khosla C, Cane DE. Chain elongation, macrolactonization, and hydrolysis of natural and reduced hexaketide substrates by the picromycin/methymycin polyketide synthase. Angewandte Chemie (International Ed. in English). 44: 7557-60. PMID 16247819 DOI: 10.1002/anie.200502246  1
2005 Vedula LS, Cane DE, Christianson DW. Role of arginine-304 in the diphosphate-triggered active site closure mechanism of trichodiene synthase. Biochemistry. 44: 12719-27. PMID 16171386 DOI: 10.1021/bi0510476  1
2005 Schnarr NA, Chen AY, Cane DE, Khosla C. Analysis of covalently bound polyketide intermediates on 6-deoxyerythronolide B synthase by tandem proteolysis-mass spectrometry. Biochemistry. 44: 11836-42. PMID 16128585 DOI: 10.1021/bi0510781  1
2005 Vedula LS, Rynkiewicz MJ, Pyun HJ, Coates RM, Cane DE, Christianson DW. Molecular recognition of the substrate diphosphate group governs product diversity in trichodiene synthase mutants. Biochemistry. 44: 6153-63. PMID 15835903 DOI: 10.1021/bi050059o  1
2004 Wu J, Kinoshita K, Khosla C, Cane DE. Biochemical analysis of the substrate specificity of the beta-ketoacyl-acyl carrier protein synthase domain of module 2 of the erythromycin polyketide synthase. Biochemistry. 43: 16301-10. PMID 15610024 DOI: 10.1021/bi048147g  1
2004 Kim CY, Alekseyev VY, Chen AY, Tang Y, Cane DE, Khosla C. Reconstituting modular activity from separated domains of 6-deoxyerythronolide B synthase. Biochemistry. 43: 13892-8. PMID 15518537 DOI: 10.1021/bi048418n  1
2004 Richard SB, Lillo AM, Tetzlaff CN, Bowman ME, Noel JP, Cane DE. Kinetic analysis of Escherichia coli 2-C-methyl-D-erythritol-4-phosphate cytidyltransferase, wild type and mutants, reveals roles of active site amino acids. Biochemistry. 43: 12189-97. PMID 15379557 DOI: 10.1021/bi0487241  1
2004 Cane DE. Back to basics; assigning biochemical function in the post-genomic era. Chemistry & Biology. 11: 741-3. PMID 15217605 DOI: 10.1016/j.chembiol.2004.06.001  1
2004 Felicetti B, Cane DE. Aristolochene synthase: mechanistic analysis of active site residues by site-directed mutagenesis. Journal of the American Chemical Society. 126: 7212-21. PMID 15186158 DOI: 10.1021/ja0499593  1
2004 Banks J, Cane DE. Biosynthesis of vitamin B6: direct identification of the product of the PdxA-catalyzed oxidation of 4-hydroxy-l-threonine-4-phosphate using electrospray ionization mass spectrometry. Bioorganic & Medicinal Chemistry Letters. 14: 1633-6. PMID 15026039 DOI: 10.1016/j.bmcl.2004.01.065  1
2004 He X, Cane DE. Mechanism and stereochemistry of the germacradienol/germacrene D synthase of Streptomyces coelicolor A3(2). Journal of the American Chemical Society. 126: 2678-9. PMID 14995166 DOI: 10.1021/ja039929k  1
2003 Kumar P, Koppisch AT, Cane DE, Khosla C. Enhancing the modularity of the modular polyketide synthases: transacylation in modular polyketide synthases catalyzed by malonyl-CoA:ACP transacylase. Journal of the American Chemical Society. 125: 14307-12. PMID 14624579 DOI: 10.1021/ja037429l  1
2003 Kinoshita K, Pfeifer BA, Khosla C, Cane DE. Precursor-Directed polyketide biosynthesis in Escherichia coli. Bioorganic & Medicinal Chemistry Letters. 13: 3701-4. PMID 14552761 DOI: 10.1016/j.bmcl.2003.08.008  1
2003 Watanabe K, Wang CC, Boddy CN, Cane DE, Khosla C. Understanding substrate specificity of polyketide synthase modules by generating hybrid multimodular synthases. The Journal of Biological Chemistry. 278: 42020-6. PMID 12923197 DOI: 10.1074/jbc.M305339200  1
2003 Sivaraman J, Li Y, Banks J, Cane DE, Matte A, Cygler M. Crystal structure of Escherichia coli PdxA, an enzyme involved in the pyridoxal phosphate biosynthesis pathway. The Journal of Biological Chemistry. 278: 43682-90. PMID 12896974 DOI: 10.1074/jbc.M306344200  1
2003 Yin Y, Lu H, Khosla C, Cane DE. Expression and kinetic analysis of the substrate specificity of modules 5 and 6 of the picromycin/methymycin polyketide synthase. Journal of the American Chemical Society. 125: 5671-6. PMID 12733905 DOI: 10.1021/ja034574q  1
2003 Hans M, Hornung A, Dziarnowski A, Cane DE, Khosla C. Mechanistic analysis of acyl transferase domain exchange in polyketide synthase modules. Journal of the American Chemical Society. 125: 5366-74. PMID 12720450 DOI: 10.1021/ja029539i  1
2003 Kumar P, Li Q, Cane DE, Khosla C. Intermodular communication in modular polyketide synthases: structural and mutational analysis of linker mediated protein-protein recognition. Journal of the American Chemical Society. 125: 4097-102. PMID 12670230 DOI: 10.1021/ja0297537  1
2003 Lillo AM, Tetzlaff CN, Sangari FJ, Cane DE. Functional expression and characterization of EryA, the erythritol kinase of Brucella abortus, and enzymatic synthesis of L-erythritol-4-phosphate. Bioorganic & Medicinal Chemistry Letters. 13: 737-9. PMID 12639570 DOI: 10.1016/S0960-894X(02)01032-6  1
2003 Cane DE, Watt RM. Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. 100: 1547-51. PMID 12556563 DOI: 10.1073/pnas.0337625100  1
2003 Liou GF, Lau J, Cane DE, Khosla C. Quantitative analysis of loading and extender acyltransferases of modular polyketide synthases. Biochemistry. 42: 200-7. PMID 12515555 DOI: 10.1021/bi0268100  1
2003 Kinoshita K, Khosla C, Cane DE. Precursor-Directed Biosynthesis: Stereospecificity for Branched-Chain Diketides of the β-Ketoacyl-ACP Synthase Domain 2 of 6-Deoxyerythronolide B Synthase Helvetica Chimica Acta. 86: 3889-3907. DOI: 10.1002/hlca.200390327  1
2003 Cane DE. Mechanism and structure of biosynthetic enzymes. The biosynthesis of vitamin B6 Chimia. 57: 75-76.  1
2002 Tsai SC, Lu H, Cane DE, Khosla C, Stroud RM. Insights into channel architecture and substrate specificity from crystal structures of two macrocycle-forming thioesterases of modular polyketide synthases. Biochemistry. 41: 12598-606. PMID 12379102 DOI: 10.1021/bi0260177  1
2002 Lu H, Tsai SC, Khosla C, Cane DE. Expression, site-directed mutagenesis, and steady state kinetic analysis of the terminal thioesterase domain of the methymycin/picromycin polyketide synthase. Biochemistry. 41: 12590-7. PMID 12379101 DOI: 10.1021/bi026006d  1
2002 Yeh JI, Du S, Pohl E, Cane DE. Multistate binding in pyridoxine 5'-phosphate synthase: 1.96 A crystal structure in complex with 1-deoxy-D-xylulose phosphate. Biochemistry. 41: 11649-57. PMID 12269807 DOI: 10.1021/bi026292t  1
2002 Seemann M, Zhai G, de Kraker JW, Paschall CM, Christianson DW, Cane DE. Pentalenene synthase. Analysis of active site residues by site-directed mutagenesis. Journal of the American Chemical Society. 124: 7681-9. PMID 12083921 DOI: 10.1021/ja026058q  1
2002 Wu N, Cane DE, Khosla C. Quantitative analysis of the relative contributions of donor acyl carrier proteins, acceptor ketosynthases, and linker regions to intermodular transfer of intermediates in hybrid polyketide synthases. Biochemistry. 41: 5056-66. PMID 11939803 DOI: 10.1021/bi012086u  1
2002 Cane DE, Kudo F, Kinoshita K, Khosla C. Precursor-directed biosynthesis: biochemical basis of the remarkable selectivity of the erythromycin polyketide synthase toward unsaturated triketides. Chemistry & Biology. 9: 131-42. PMID 11841945 DOI: 10.1016/S1074-5521(02)00089-3  1
2002 Rynkiewicz MJ, Cane DE, Christianson DW. X-ray crystal structures of D100E trichodiene synthase and its pyrophosphate complex reveal the basis for terpene product diversity. Biochemistry. 41: 1732-41. PMID 11827517 DOI: 10.1021/bi011960g  1
2002 Richard SB, Ferrer JL, Bowman ME, Lillo AM, Tetzlaff CN, Cane DE, Noel JP. Structure and mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. An enzyme in the mevalonate-independent isoprenoid biosynthetic pathway. The Journal of Biological Chemistry. 277: 8667-72. PMID 11786530 DOI: 10.1074/jbc.C100739200  1
2001 Tsai SC, Miercke LJ, Krucinski J, Gokhale R, Chen JC, Foster PG, Cane DE, Khosla C, Stroud RM. Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel. Proceedings of the National Academy of Sciences of the United States of America. 98: 14808-13. PMID 11752428 DOI: 10.1073/pnas.011399198  1
2001 Rynkiewicz MJ, Cane DE, Christianson DW. Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade Proceedings of the National Academy of Sciences of the United States of America. 98: 13543-13548. PMID 11698643 DOI: 10.1073/pnas.231313098  1
2001 Chen H, O'Connor S, Cane DE, Walsh CT. Epothilone biosynthesis: assembly of the methylthiazolylcarboxy starter unit on the EpoB subunit. Chemistry & Biology. 8: 899-912. PMID 11564558 DOI: 10.1016/S1074-5521(01)00064-3  1
2001 Lombó F, Pfeifer B, Leaf T, Ou S, Kim YS, Cane DE, Licari P, Khosla C. Enhancing the atom economy of polyketide biosynthetic processes through metabolic engineering. Biotechnology Progress. 17: 612-7. PMID 11485419 DOI: 10.1021/bp010045j  1
2001 Kinoshita K, Williard PG, Khosla C, Cane DE. Precursor-directed biosynthesis of 16-membered macrolides by the erythromycin polyketide synthase. Journal of the American Chemical Society. 123: 2495-502. PMID 11456917 DOI: 10.1021/ja004139l  1
2001 Wu N, Tsuji SY, Cane DE, Khosla C. Assessing the balance between protein-protein interactions and enzyme-substrate interactions in the channeling of intermediates between polyketide synthase modules. Journal of the American Chemical Society. 123: 6465-74. PMID 11439032 DOI: 10.1021/ja010219t  1
2001 Richard SB, Bowman ME, Kwiatkowski W, Kang I, Chow C, Lillo AM, Cane DE, Noel JP. Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis. Nature Structural Biology. 8: 641-8. PMID 11427897 DOI: 10.1038/89691  1
2001 Yin Y, Gokhale R, Khosla C, Cane DE. Erythromycin biosynthesis. The 4-pro-S hydride of NADPH is utilized for ketoreduction by both module 5 and module 6 of the 6-deoxyerythronolide B synthase. Bioorganic & Medicinal Chemistry Letters. 11: 1477-9. PMID 11412964 DOI: 10.1016/S0960-894X(00)00529-1  1
2001 Cane DE, Chow C, Lillo A, Kang I. Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor. Bioorganic & Medicinal Chemistry. 9: 1467-77. PMID 11408165 DOI: 10.1016/S0968-0896(01)00050-5  1
2001 Pohl NL, Hans M, Lee HY, Kim YS, Cane DE, Khosla C. Remarkably broad substrate tolerance of malonyl-CoA synthetase, an enzyme capable of intracellular synthesis of polyketide precursors. Journal of the American Chemical Society. 123: 5822-3. PMID 11403625 DOI: 10.1021/ja0028368  1
2001 Tsuji SY, Cane DE, Khosla C. Selective protein-protein interactions direct channeling of intermediates between polyketide synthase modules. Biochemistry. 40: 2326-31. PMID 11327852 DOI: 10.1021/bi002463n  1
2001 Pfeifer BA, Admiraal SJ, Gramajo H, Cane DE, Khosla C. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli. Science (New York, N.Y.). 291: 1790-2. PMID 11230695 DOI: 10.1126/science.1058092  1
2000 Lau J, Cane DE, Khosla C. Substrate specificity of the loading didomain of the erythromycin polyketide synthase. Biochemistry. 39: 10514-20. PMID 10956042 DOI: 10.1021/bi000602v  1
2000 Caruthers JM, Kang I, Rynkiewicz MJ, Cane DE, Christianson DW. Crystal structure determination of aristolochene synthase from the blue cheese mold, Penicillium roqueforti. The Journal of Biological Chemistry. 275: 25533-9. PMID 10825154 DOI: 10.1074/jbc.M000433200  1
2000 Cane DE, Kang I. Aristolochene synthase: purification, molecular cloning, high-level expression in Escherichia coli, and characterization of the Aspergillus terreus cyclase. Archives of Biochemistry and Biophysics. 376: 354-64. PMID 10775423 DOI: 10.1006/abbi.2000.1734  1
2000 Cane DE. Perspectives: biosynthetic pathways. Biosynthesis meets bioinformatics. Science (New York, N.Y.). 287: 818-9. PMID 10691559 DOI: 10.1126/science.287.5454.818  1
2000 Cane DE, Ke N. Epicubenol synthase. Origin of the oxygen atom of a bacterial sesquiterpene alcohol. Bioorganic & Medicinal Chemistry Letters. 10: 105-7. PMID 10673090  1
2000 Cane DE, Du S, Spenser ID. Biosynthesis of vitamin B 6: Origin of the oxygen atoms of pyridoxol phosphate [1] Journal of the American Chemical Society. 122: 4213-4214. DOI: 10.1021/ja000224h  1
2000 Wu N, Kudo F, Cane DE, Khosla C. Analysis of the molecular recognition features of individual modules derived from the erythromycin polyketide synthase Journal of the American Chemical Society. 122: X. DOI: 10.1021/ja000023d  1
2000 Wendt KU, Poralla K, Shulz GE, Starks CM, Bock K, Chappell J, Noel J, Zhai G, Cane DE, Christianson DW. Structures of enzymes involved in terpenoid biosynthesis Chemtracts. 13: 844-852.  1
1999 Khosla C, Gokhale RS, Jacobsen JR, Cane DE. Tolerance and specificity of polyketide synthases. Annual Review of Biochemistry. 68: 219-53. PMID 10872449 DOI: 10.1146/annurev.biochem.68.1.219  1
1999 Cane DE, Walsh CT. The parallel and convergent universes of polyketide synthases and nonribosomal peptide synthetases. Chemistry & Biology. 6: R319-25. PMID 10631508 DOI: 10.1016/S1074-5521(00)80001-0  1
1999 Cane DE, Bowser TE. Trichodiene synthase: mechanism-based inhibition of a sesquiterpene cyclase. Bioorganic & Medicinal Chemistry Letters. 9: 1127-32. PMID 10328298 DOI: 10.1016/S0960-894X(99)00149-3  1
1999 Gokhale RS, Tsuji SY, Cane DE, Khosla C. Dissecting and exploiting intermodular communication in polyketide synthases. Science (New York, N.Y.). 284: 482-5. PMID 10205055 DOI: 10.1126/science.284.5413.482  1
1999 Gokhale RS, Hunziker D, Cane DE, Khosla C. Mechanism and specificity of the terminal thioesterase domain from the erythromycin polyketide synthase. Chemistry & Biology. 6: 117-25. PMID 10021418 DOI: 10.1016/S1074-5521(99)80008-8  1
1999 Lau J, Fu H, Cane DE, Khosla C. Dissecting the role of acyltransferase domains of modular polyketide synthases in the choice and stereochemical fate of extender units. Biochemistry. 38: 1643-51. PMID 9931032 DOI: 10.1021/bi9820311  1
1999 Cane DE, Du S, Robinson JK, Hsiung Y, Spenser ID. Biosynthesis of vitamin B6: Enzymatic conversion of 1-deoxy-D-xylulose- 5-phosphate to pyridoxol phosphate [14] Journal of the American Chemical Society. 121: 7722-7723. DOI: 10.1021/ja9914947  1
1999 Hunziker D, Wu N, Kenoshita K, Cane DE, Khosla C. Precursor directed biosynthesis of novel 6-deoxyerythronolide B analogs containing non-natural oxygen substituents and reactive functionalities Tetrahedron Letters. 40: 635-638. DOI: 10.1016/S0040-4039(98)02507-6  1
1998 Graziani EI, Cane DE, Betlach MC, Kealey JT, McDaniel R. Macrolide biosynthesis: a single cytochrome P450, PicK, is responsible for the hydroxylations that generate methymycin, neomethymycin, and picromycin in Streptomyces venezuelae. Bioorganic & Medicinal Chemistry Letters. 8: 3117-20. PMID 9873687  1
1998 Jacobsen JR, Keatinge-Clay AT, Cane DE, Khosla C. Precursor-directed biosynthesis of 12-ethyl erythromycin. Bioorganic & Medicinal Chemistry. 6: 1171-7. PMID 9784859 DOI: 10.1016/S0968-0896(98)00081-9  1
1998 Cane DE, Walsh CT, Khosla C. Harnessing the biosynthetic code: combinations, permutations, and mutations. Science (New York, N.Y.). 282: 63-8. PMID 9756477 DOI: 10.1126/science.282.5386.63  1
1998 Jacobsen JR, Cane DE, Khosla C. Spontaneous priming of a downstream module in 6-deoxyerythronolide B synthase leads to polyketide biosynthesis. Biochemistry. 37: 4928-34. PMID 9538011 DOI: 10.1021/bi9729920  1
1998 Gokhale RS, Lau J, Cane DE, Khosla C. Functional orientation of the acyltransferase domain in a module of the erythromycin polyketide synthase. Biochemistry. 37: 2524-8. PMID 9485401 DOI: 10.1021/bi971887n  1
1998 Pohl NL, Gokhale RS, Cane DE, Khosla C. Synthesis and incorporation of an N-acetylcysteamine analogue of methylmalonyl-CoA by a modular polyketide synthase [13] Journal of the American Chemical Society. 120: 11206-11207. DOI: 10.1021/ja9830290  1
1998 Jacobsen JR, Cane DE, Khosla C. Dissecting the evolutionary relationship between 14-membered and 16- membered macrolides [14] Journal of the American Chemical Society. 120: 9096-9097. DOI: 10.1021/ja9819514  1
1998 McPherson M, Khosla C, Cane DE. Erythromycin biosynthesis: The β-ketoreductase domains catalyze the stereospecific transfer of the 4-pro-S hydride of NADPH [2] Journal of the American Chemical Society. 120: 3267-3268. DOI: 10.1021/ja980028z  1
1998 Cane DE, Graziani EI. Methymycin biosynthesis. Isolation of P450 monooxygenase activity in a cell-free system from Streptomyces venezuelae [17] Journal of the American Chemical Society. 120: 2682-2683. DOI: 10.1021/ja974321v  1
1998 Cane DE, Hsiung Y, Cornish JA, Robinson JK, Spenser ID. Biosynthesis of vitamin B6: The oxidation of 4-(phosphohydroxy)-L-threonine by PdxA Journal of the American Chemical Society. 120: 1936-1937. DOI: 10.1021/ja9742085  1
1998 Kao CM, McPherson M, McDaniel RN, Fu H, Cane DE, Khosla C. Alcohol stereochemistry in polyketide backbones is controlled by the β-ketoreductase domains of modular polyketide synthases Journal of the American Chemical Society. 120: 2478-2479. DOI: 10.1021/ja973913a  1
1997 Chuck JA, McPherson M, Huang H, Jacobsen JR, Khosla C, Cane DE. Molecular recognition of diketide substrates by a beta-ketoacyl-acyl carrier protein synthase domain within a bimodular polyketide synthase. Chemistry & Biology. 4: 757-66. PMID 9375254  1
1997 Lesburg CA, Zhai G, Cane DE, Christianson DW. Crystal structure of pentalenene synthase: mechanistic insights on terpenoid cyclization reactions in biology. Science (New York, N.Y.). 277: 1820-4. PMID 9295272 DOI: 10.1126/science.277.5333.1820  1
1997 Jacobsen JR, Hutchinson CR, Cane DE, Khosla C. Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase. Science (New York, N.Y.). 277: 367-9. PMID 9219693 DOI: 10.1126/science.277.5324.367  1
1997 Cane DE, Chiu HT, Liang PH, Anderson KS. Pre-steady-state kinetic analysis of the trichodiene synthase reaction pathway. Biochemistry. 36: 8332-9. PMID 9204880 DOI: 10.1021/bi963018o  1
1997 Pieper R, Gokhale RS, Luo G, Cane DE, Khosla C. Purification and characterization of bimodular and trimodular derivatives of the erythromycin polyketide synthase. Biochemistry. 36: 1846-51. PMID 9048569 DOI: 10.1021/bi962324z  1
1997 Kao CM, McPherson M, McDaniel RN, Fu H, Cane DE, Khosla C. Gain of function mutagenesis of the erythromycin polyketide synthase. 2. Engineered biosynthesis of an eight-membered ring tetraketide lactone Journal of the American Chemical Society. 119: 11339-11340. DOI: 10.1021/ja972609e  1
1997 McDaniel R, Kao CM, Fu H, Hevezi P, Gustafsson C, Betlach M, Ashley G, Cane DE, Khosla C. Gain-of-function mutagenesis of a modular polyketide synthase Journal of the American Chemical Society. 119: 4309-4310. DOI: 10.1021/ja9702531  1
1996 Hill AM, Cane DE, Mau CJ, West CA. High level expression of Ricinus communis casbene synthase in Escherichia coli and characterization of the recombinant enzyme. Archives of Biochemistry and Biophysics. 336: 283-9. PMID 8954576 DOI: 10.1006/abbi.1996.0559  1
1996 Tsukamoto N, Chuck JA, Luo G, Kao CM, Khosla C, Cane DE. 6-deoxyerythronolide B synthase 1 is specifically acylated by a diketide intermediate at the beta-ketoacyl-acyl carrier protein synthase domain of module 2. Biochemistry. 35: 15244-8. PMID 8952473 DOI: 10.1021/bi961972f  1
1996 Bedford D, Jacobsen JR, Luo G, Cane DE, Khosla C. A functional chimeric modular polyketide synthase generated via domain replacement Chemistry and Biology. 3: 827-831. PMID 8939701  1
1996 Luo G, Pieper R, Rosa A, Khosla C, Cane DE. Erythromycin biosynthesis: Exploiting the catalytic versatility of the modular polyketide synthase Bioorganic and Medicinal Chemistry. 4: 995-999. PMID 8831969 DOI: 10.1016/0968-0896(96)00096-X  1
1996 Cane DE, Xue Q, Fitzsimons BC. Trichodiene synthase. Probing the role of the highly conserved aspartate-rich region by site-directed mutagenesis Biochemistry. 35: 12369-12376. PMID 8823172 DOI: 10.1021/bi961344y  1
1996 Kao CM, Pieper R, Cane DE, Khosla C. Evidence for two catalytically independent clusters of active sites in a functional modular polyketide synthase Biochemistry. 35: 12363-12368. PMID 8823171 DOI: 10.1021/bi9616312  1
1996 Cane DE, Tsantrizos YS. Aristolochene synthase. Elucidation of the cryptic germacrene A synthase activity using the anomalous substrate dihydrofarnesyl diphosphate Journal of the American Chemical Society. 118: 10037-10040. DOI: 10.1021/ja961981b  1
1996 Cane DE, Xue Q, Van Epp JE, Tsantrizos YS. Enzymatic formation of isochamigrene, a novel sesquiterpene, by alteration of the aspartate-rich region of trichodiene synthase Journal of the American Chemical Society. 118: 8499-8500. DOI: 10.1021/ja961897w  1
1996 Kao CM, Luo G, Katz L, Cane DE, Khosla C. Engineered biosynthesis of structurally diverse tetraketides by a trimodular polyketide synthase Journal of the American Chemical Society. 118: 9184-9185. DOI: 10.1021/ja9617552  1
1996 Cane DE, Xue Q. Trichodiene synthase. Enzymatic formation of multiple sesquiterpenes by alteration of the cyclase active site Journal of the American Chemical Society. 118: 1563-1564. DOI: 10.1021/ja953751n  1
1996 Pieper R, Kao C, Khosla C, Luo G, Cane DE. Specificity and versatility in erythromycin biosynthesis Chemical Society Reviews. 25: 297-302.  1
1995 Cane DE. Isoprenoid antibiotics Biotechnology (Reading, Mass.). 28: 633-655. PMID 8688640  1
1995 Lesburg CA, Lloyd MD, Cane DE, Christianson DW. Crystallization and preliminary X-ray diffraction analysis of recombinant pentalenene synthase. Protein Science : a Publication of the Protein Society. 4: 2436-8. PMID 8563643 DOI: 10.1002/pro.5560041124  1
1995 Cane DE. Trichodiene synthase. Substrate specificity and inhibition Biochemistry®. 34: 2471-2479. PMID 7873526  1
1995 Cane DE. Overproduction and characterization of the erythromycin c-12 hydroxylase, eryk Biochemistry®. 34: 1858-1866. PMID 7849045  1
1995 Cane DE, Luo G, Khosla C, Kao CM, Katz L. Erythromycin biosynthesis. Highly efficient incorporation of polyketide chain elongation intermediates into 6-deoxyerythronolide B in an engineered streptomyces host Journal of Antibiotics. 48: 647-651. PMID 7649863  1
1995 Pieper R, Luo G, Cane DE, Khosla C. Cell-free synthesis of polyketides by recombinant erythromycin polyketide synthases Nature. 378: 263-266. PMID 7477343  1
1995 Cane DE, Luo G. Biosynthesis of polyketide antibiotics. Incorporation of a pentaketide chain elongation intermediate into nargenicin Journal of the American Chemical Society. 117: 6633-6634. DOI: 10.1021/ja00129a044  1
1995 Cane DE, Tandon M. Epicubenol synthase and the stereochemistry of the enzymatic cyclization of farnesyl and nerolidyl diphosphate Journal of the American Chemical Society. 117: 5602-5603. DOI: 10.1021/ja00125a029  1
1995 Cane DE. Trichodiene synthase. Identification of active site residues by site-directed mutagenesis Biochemistry®. 34: 2480-2488.  1
1995 Pieper R, Luo G, Cane DE, Khosla C. Remarkably broad substrate specificity of a modular polyketide synthase in a cell-free system Journal of the American Chemical Society. 117: 11373-11374.  1
1995 Kao CM, Luo G, Katz L, Cane DE, Khosla C. Manipulation of macrolide ring size by directed mutagenesis of a modular polyketide synthase Journal of the American Chemical Society. 117: 9105-9106.  1
1994 Cane DE, Sohng JK. Inhibition of glyceraldehyde-3-phosphate dehydrogenase by pentalenolactone. 2. Identification of the site of alkylation by tetrahydropentalenolactone Biochemistry®. 6524-6530. PMID 8204587  1
1994 Cane DE, Sohng JK, Lamberson CR, Rudnicki SM, Wu Z, Lloyd MD, Oliver JS, Hubbard BR. Pentalenene synthase. Purification, molecular cloning, sequencing, and high-level expression in Escherichia coli of a terpenoid cyclase from Streptomyces UC5319. Biochemistry. 33: 5846-57. PMID 8180213  1
1994 Cane DE, Bryant C. Aristolochene synthase. Mechanism-based inhibition of a terpenoid cyclase Journal of the American Chemical Society. 116: 12063-12064.  1
1994 Kao CM, Luo G, Katz L, Cane DE, Khosla C. Engineered biosynthesis of a triketide lactone from an incomplete modular polyketide synthase Journal of the American Chemical Society. 116: 11612-11613.  1
1994 Cane DE, Yang G. Trichodiene synthase. Stereochemical studies of the cryptic allylic diphosphate isomerase activity using an anomalous substrate Journal of Organic Chemistry. 59: 5794-5798.  1
1977 Cane DE, Buchwald SL. Application of deuterium magnetic resonance to biosynthetic studies. 1. Biosynthesis of ovalicin. Journal of the American Chemical Society. 99: 6132-4. PMID 893881  1
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