Edward A. Lemke - Publications

Affiliations: 
EMBL Heidelberg 
Area:
biophysics, chemical biology

67 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2019 Celetti G, Paci G, Caria J, VanDelinder V, Bachand G, Lemke EA. The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes. The Journal of Cell Biology. PMID 31723007 DOI: 10.1083/jcb.201907157  1
2019 Chowdhury A, Yu M, Lemke EA. Phase Separation Comes of Age: From Phenomenology to Single Molecules. Molecular Cell. 74: 413-415. PMID 31051137 DOI: 10.1016/j.molcel.2019.04.016  0.6
2019 Lu M, Ma X, Castillo-Menendez LR, Gorman J, Alsahafi N, Ermel U, Terry DS, Chambers M, Peng D, Zhang B, Zhou T, Reichard N, Wang K, Grover JR, Carman BP, ... ... Lemke EA, et al. Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET. Nature. PMID 30971821 DOI: 10.1038/s41586-019-1101-y  0.36
2019 Reinkemeier CD, Girona GE, Lemke EA. Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes. Science (New York, N.Y.). 363. PMID 30923194 DOI: 10.1126/science.aaw2644  0.76
2019 Lemke EA, Chowdhury A, Kovalenko SA, Aramburu IV, Tan PS, Ernsting NP. Mechanism dependent modulation of ultrafast interfacial water dynamics in intrinsically disordered protein complexes. Angewandte Chemie (International Ed. in English). PMID 30703278 DOI: 10.1002/anie.201813354  0.76
2018 Tan PS, Lemke EA. Probing Differential Binding Mechanisms of Phenylalanine-Glycine-Rich Nucleoporins by Single-Molecule FRET. Methods in Enzymology. 611: 327-346. PMID 30471692 DOI: 10.1016/bs.mie.2018.08.034  0.32
2018 Fuertes G, Banterle N, Ruff KM, Chowdhury A, Pappu RV, Svergun DI, Lemke EA. Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water". Science (New York, N.Y.). 361. PMID 30166461 DOI: 10.1126/science.aau8230  0.6
2018 Mikhaleva S, Lemke EA. Beyond the Transport Function of Import Receptors: What's All the FUS about? Cell. 173: 549-553. PMID 29677508 DOI: 10.1016/j.cell.2018.04.002  0.44
2018 Kormos A, Koehler C, Fodor E, Rutkai Z, Martin M, Mező G, Lemke E, Kele P. Bistetrazine-cyanines as double-clicking fluorogenic two-point binder or crosslinker probes. Chemistry (Weinheim An Der Bergstrasse, Germany). PMID 29676491 DOI: 10.1002/chem.201800910  0.44
2018 Tan PS, Aramburu IV, Mercadante D, Tyagi S, Chowdhury A, Spitz D, Shammas SL, Gräter F, Lemke EA. Two Differential Binding Mechanisms of FG-Nucleoporins and Nuclear Transport Receptors. Cell Reports. 22: 3660-3671. PMID 29590630 DOI: 10.1016/j.celrep.2018.03.022  0.76
2018 Kozma E, Paci G, Estrada Girona G, Lemke EA, Kele P. Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins. Methods in Molecular Biology (Clifton, N.J.). 1728: 337-363. PMID 29405009 DOI: 10.1007/978-1-4939-7574-7_22  1
2018 Koehler C, Lemke EA. MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells. Methods in Molecular Biology (Clifton, N.J.). 1728: 297-311. PMID 29405006 DOI: 10.1007/978-1-4939-7574-7_19  0.44
2017 Gupta K, Watson AA, Baptista T, Scheer E, Chambers AL, Koehler C, Zou J, Obong-Ebong I, Kandiah E, Temblador A, Round A, Forest E, Man P, Bieniossek C, Laue ED, ... Lemke EA, et al. Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID. Elife. 6. PMID 29111974 DOI: 10.7554/eLife.30395  0.44
2017 Warner JB, Ruff K, Tan PS, Lemke EA, Pappu RV, Lashuel HA. Monomeric huntingtin exon 1 has similar overall structural features for wild type and pathological polyglutamine lengths. Journal of the American Chemical Society. PMID 28937758 DOI: 10.1021/jacs.7b06659  0.32
2017 Mercadante D, Wagner JA, Aramburu IV, Lemke EA, Gräter F. Sampling long versus short range interactions defines the ability of force fields to reproduce the dynamics of intrinsically disordered proteins. Journal of Chemical Theory and Computation. PMID 28805390 DOI: 10.1021/acs.jctc.7b00143  0.76
2017 Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, ... Lemke EA, et al. Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proceedings of the National Academy of Sciences of the United States of America. PMID 28716919 DOI: 10.1073/pnas.1704692114  0.6
2017 Aramburu IV, Lemke EA. Floppy but not sloppy: Interaction mechanism of FG-Nucleoporins and nuclear transport receptors. Seminars in Cell & Developmental Biology. PMID 28669824 DOI: 10.1016/j.semcdb.2017.06.026  0.76
2017 Sakin V, Hanne J, Dunder J, Anders-Össwein M, Laketa V, Nikić I, Kräusslich HG, Lemke EA, Müller B. A Versatile Tool for Live-Cell Imaging and Super-Resolution Nanoscopy Studies of HIV-1 Env Distribution and Mobility. Cell Chemical Biology. PMID 28457706 DOI: 10.1016/j.chembiol.2017.04.007  0.6
2017 Demeter O, Kormos A, Koehler C, Mezõ G, Németh K, Kozma E, Takacs L, Lemke EA, Kele P. BISAZIDE CYANINE DYES AS FLUOROGENIC PROBES FOR BIS-CYCLOOCTYNYLATED PEPTIDE TAGS AND AS FLUOROGENIC CROSSLINKERS OF CYCLOOCTYNYLATED PROTEINS. Bioconjugate Chemistry. PMID 28441009 DOI: 10.1021/acs.bioconjchem.7b00178  0.44
2017 Paci G, Lemke EA. Shining a Light on Phase Separation in the Cell. Cell. 168: 11-13. PMID 28086083 DOI: 10.1016/j.cell.2016.12.018  1
2016 Nikić I, Estrada Girona G, Kang JH, Paci G, Mikhaleva S, Koehler C, Shymanska NV, Ventura Santos C, Spitz D, Lemke EA. Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy. Angewandte Chemie (International Ed. in English). PMID 27804198 DOI: 10.1002/anie.201608284  1
2016 Koehler C, Sauter PF, Wawryszyn M, Girona GE, Gupta K, Landry JJ, Fritz MH, Radic K, Hoffmann JE, Chen ZA, Zou J, Tan PS, Galik B, Junttila S, Stolt-Bergner P, ... ... Lemke EA, et al. Genetic code expansion for multiprotein complex engineering. Nature Methods. PMID 27749839 DOI: 10.1038/nmeth.4032  0.76
2016 Kozma E, Nikic I, Varga BR, Aramburu IV, Kang JH, Fackler OT, Lemke EA, Kele P. Hydrophilic trans-Cyclooctenylated Non-Canonical Amino Acids for Fast Intracellular Protein Labeling. Chembiochem : a European Journal of Chemical Biology. PMID 27223658 DOI: 10.1002/cbic.201600284  1
2016 Knorr G, Kozma E, Herner A, Lemke EA, Kele P. New Red-Emitting Tetrazine-Phenoxazine Fluorogenic Labels for Live-Cell Intracellular Bioorthogonal Labeling Schemes. Chemistry (Weinheim An Der Bergstrasse, Germany). PMID 27218228 DOI: 10.1002/chem.201600590  1
2016 Sakin V, Paci G, Lemke EA, Müller B. Labeling of virus components for advanced, quantitative imaging analyses. Febs Letters. PMID 26987299 DOI: 10.1002/1873-3468.12131  1
2015 Vreja IC, Nikić I, Göttfert F, Bates M, Kröhnert K, Outeiro TF, Hell SW, Lemke EA, Rizzoli SO. Super-resolution Microscopy of Clickable Amino Acids Reveals the Effects of Fluorescent Protein Tagging on Protein Assemblies. Acs Nano. PMID 26498474 DOI: 10.1021/acsnano.5b04434  1
2015 Milles S, Mercadante D, Aramburu IV, Jensen MR, Banterle N, Koehler C, Tyagi S, Clarke J, Shammas SL, Blackledge M, Gräter F, Lemke EA. Plasticity of an Ultrafast Interaction between Nucleoporins and Nuclear Transport Receptors. Cell. 163: 734-45. PMID 26456112 DOI: 10.1016/j.cell.2015.09.047  1
2015 Delaforge E, Milles S, Bouvignies G, Bouvier D, Boivin S, Salvi N, Maurin D, Martel A, Round A, Lemke EA, Ringkjøbing Jensen M, Hart DJ, Blackledge M. Large-Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α. Journal of the American Chemical Society. PMID 26424125 DOI: 10.1021/jacs.5b07765  1
2015 von Appen A, Kosinski J, Sparks L, Ori A, DiGuilio AL, Vollmer B, Mackmull MT, Banterle N, Parca L, Kastritis P, Buczak K, Mosalaganti S, Hagen W, Andres-Pons A, Lemke EA, et al. In situ structural analysis of the human nuclear pore complex. Nature. 526: 140-3. PMID 26416747 DOI: 10.1038/nature15381  1
2015 Nikić I, Lemke EA. Genetic code expansion enabled site-specific dual-color protein labeling: superresolution microscopy and beyond. Current Opinion in Chemical Biology. 28: 164-73. PMID 26302384 DOI: 10.1016/j.cbpa.2015.07.021  0.44
2015 Wagner JA, Mercadante D, Nikić I, Lemke EA, Gräter F. Origin of Orthogonality of Strain-Promoted Click Reactions. Chemistry (Weinheim An Der Bergstrasse, Germany). 21: 12431-5. PMID 26178299 DOI: 10.1002/chem.201501727  0.44
2015 Hoffmann JE, Plass T, Nikić I, Aramburu IV, Koehler C, Gillandt H, Lemke EA, Schultz C. Highly Stable trans-Cyclooctene Amino Acids for Live-Cell Labeling. Chemistry (Weinheim An Der Bergstrasse, Germany). 21: 12266-70. PMID 26177861 DOI: 10.1002/chem.201501647  1
2015 Mercadante D, Milles S, Fuertes G, Svergun DI, Lemke EA, Gräter F. Kirkwood-Buff Approach Rescues Overcollapse of a Disordered Protein in Canonical Protein Force Fields. The Journal of Physical Chemistry. B. 119: 7975-84. PMID 26030189 DOI: 10.1021/acs.jpcb.5b03440  0.44
2015 Niki? I, Kang JH, Girona GE, Aramburu IV, Lemke EA. Labeling proteins on live mammalian cells using click chemistry. Nature Protocols. 10: 780-91. PMID 25906116 DOI: 10.1038/nprot.2015.045  0.76
2015 Tyagi S, Lemke EA. Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids. Current Opinion in Structural Biology. 32: 66-73. PMID 25757192 DOI: 10.1016/j.sbi.2015.02.009  1
2014 Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R. The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor. Plos Pathogens. 10: e1004228. PMID 25058163 DOI: 10.1371/journal.ppat.1004228  1
2014 Herner A, Estrada Girona G, Nikić I, Kállay M, Lemke EA, Kele P. New generation of bioorthogonally applicable fluorogenic dyes with visible excitations and large Stokes shifts. Bioconjugate Chemistry. 25: 1370-4. PMID 24932756 DOI: 10.1021/bc500235p  1
2014 Milles S, Lemke EA. Mapping multivalency and differential affinities within large intrinsically disordered protein complexes with segmental motion analysis. Angewandte Chemie (International Ed. in English). 53: 7364-7. PMID 24898547 DOI: 10.1002/anie.201403694  0.44
2014 Pavic K, Rios P, Dzeyk K, Koehler C, Lemke EA, Köhn M. Unnatural amino acid mutagenesis reveals dimerization as a negative regulatory mechanism of VHR's phosphatase activity. Acs Chemical Biology. 9: 1451-9. PMID 24798147 DOI: 10.1021/cb500240n  0.44
2014 Nikić I, Plass T, Schraidt O, SzymaÅ„ski J, Briggs JA, Schultz C, Lemke EA. Minimal tags for rapid dual-color live-cell labeling and super-resolution microscopy. Angewandte Chemie (International Ed. in English). 53: 2245-9. PMID 24474648 DOI: 10.1002/anie.201309847  1
2014 Tyagi S, VanDelinder V, Banterle N, Fuertes G, Milles S, Agez M, Lemke EA. Continuous throughput and long-term observation of single-molecule FRET without immobilization. Nature Methods. 11: 297-300. PMID 24441935 DOI: 10.1038/nmeth.2809  0.68
2013 Herner A, Nikić I, Kállay M, Lemke EA, Kele P. A new family of bioorthogonally applicable fluorogenic labels. Organic & Biomolecular Chemistry. 11: 3297-306. PMID 23535909 DOI: 10.1039/c3ob40296g  1
2013 Ori A, Banterle N, Iskar M, Andrés-Pons A, Escher C, Khanh Bui H, Sparks L, Solis-Mezarino V, Rinner O, Bork P, Lemke EA, Beck M. Cell type-specific nuclear pores: a case in point for context-dependent stoichiometry of molecular machines. Molecular Systems Biology. 9: 648. PMID 23511206 DOI: 10.1038/msb.2013.4  1
2013 Lukinavičius G, Umezawa K, Olivier N, Honigmann A, Yang G, Plass T, Mueller V, Reymond L, Corrêa IR, Luo ZG, Schultz C, Lemke EA, Heppenstall P, Eggeling C, Manley S, et al. A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nature Chemistry. 5: 132-9. PMID 23344448 DOI: 10.1038/nchem.1546  1
2013 Tyagi S, Lemke EA. Genetically encoded click chemistry for single-molecule FRET of proteins. Methods in Cell Biology. 113: 169-87. PMID 23317903 DOI: 10.1016/B978-0-12-407239-8.00009-4  1
2013 Milles S, Huy Bui K, Koehler C, Eltsov M, Beck M, Lemke EA. Facilitated aggregation of FG nucleoporins under molecular crowding conditions. Embo Reports. 14: 178-83. PMID 23238392 DOI: 10.1038/embor.2012.204  0.44
2013 Milles S, Lemke EA. What precision-protein-tuning and nano-resolved single molecule sciences can do for each other. Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology. 35: 65-74. PMID 23147190 DOI: 10.1002/bies.201200094  0.44
2012 Borrmann A, Milles S, Plass T, Dommerholt J, Verkade JM, Wiessler M, Schultz C, van Hest JC, van Delft FL, Lemke EA. Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation. Chembiochem : a European Journal of Chemical Biology. 13: 2094-9. PMID 22945333 DOI: 10.1002/cbic.201200407  0.44
2012 Krishnan R, Goodman JL, Mukhopadhyay S, Pacheco CD, Lemke EA, Deniz AA, Lindquist S. Conserved features of intermediates in amyloid assembly determine their benign or toxic states. Proceedings of the National Academy of Sciences of the United States of America. 109: 11172-7. PMID 22745165 DOI: 10.1073/pnas.1209527109  1
2012 Milles S, Koehler C, Gambin Y, Deniz AA, Lemke EA. Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range. Molecular Biosystems. 8: 2531-4. PMID 22739670 DOI: 10.1039/c2mb25135c  1
2012 Plass T, Milles S, Koehler C, SzymaÅ„ski J, Mueller R, Wiessler M, Schultz C, Lemke EA. Amino acids for Diels-Alder reactions in living cells. Angewandte Chemie (International Ed. in English). 51: 4166-70. PMID 22473599 DOI: 10.1002/anie.201108231  0.44
2012 Milles S, Tyagi S, Banterle N, Koehler C, VanDelinder V, Plass T, Neal AP, Lemke EA. Click strategies for single-molecule protein fluorescence. Journal of the American Chemical Society. 134: 5187-95. PMID 22356317 DOI: 10.1021/ja210587q  1
2011 Milles S, Lemke EA. Single molecule study of the intrinsically disordered FG-repeat nucleoporin 153. Biophysical Journal. 101: 1710-9. PMID 21961597 DOI: 10.1016/j.bpj.2011.08.025  0.44
2011 Plass T, Milles S, Koehler C, Schultz C, Lemke EA. Genetically encoded copper-free click chemistry. Angewandte Chemie (International Ed. in English). 50: 3878-81. PMID 21433234 DOI: 10.1002/anie.201008178  0.44
2011 Gambin Y, VanDelinder V, Ferreon AC, Lemke EA, Groisman A, Deniz AA. Visualizing a one-way protein encounter complex by ultrafast single-molecule mixing. Nature Methods. 8: 239-41. PMID 21297620 DOI: 10.1038/nmeth.1568  1
2009 Lemke EA, Gambin Y, Vandelinder V, Brustad EM, Liu HW, Schultz PG, Groisman A, Deniz AA. Microfluidic device for single-molecule experiments with enhanced photostability. Journal of the American Chemical Society. 131: 13610-2. PMID 19772358 DOI: 10.1021/ja9027023  1
2009 Lee HS, Guo J, Lemke EA, Dimla RD, Schultz PG. Genetic incorporation of a small, environmentally sensitive, fluorescent probe into proteins in Saccharomyces cerevisiae. Journal of the American Chemical Society. 131: 12921-3. PMID 19702307 DOI: 10.1021/ja904896s  1
2009 Gambin Y, Schug A, Lemke EA, Lavinder JJ, Ferreon AC, Magliery TJ, Onuchic JN, Deniz AA. Direct single-molecule observation of a protein living in two opposed native structures. Proceedings of the National Academy of Sciences of the United States of America. 106: 10153-8. PMID 19506258 DOI: 10.1073/pnas.0904461106  1
2009 Ferreon AC, Gambin Y, Lemke EA, Deniz AA. Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence. Proceedings of the National Academy of Sciences of the United States of America. 106: 5645-50. PMID 19293380 DOI: 10.1073/pnas.0809232106  1
2008 Brustad EM, Lemke EA, Schultz PG, Deniz AA. A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer. Journal of the American Chemical Society. 130: 17664-5. PMID 19108697 DOI: 10.1021/ja807430h  1
2008 Deniz AA, Mukhopadhyay S, Lemke EA. Single-molecule biophysics: at the interface of biology, physics and chemistry. Journal of the Royal Society, Interface / the Royal Society. 5: 15-45. PMID 17519204 DOI: 10.1098/rsif.2007.1021  1
2007 Lemke EA, Summerer D, Geierstanger BH, Brittain SM, Schultz PG. Control of protein phosphorylation with a genetically encoded photocaged amino acid Nature Chemical Biology. 3: 769-772. PMID 17965709 DOI: 10.1038/nchembio.2007.44  1
2007 Mukhopadhyay S, Krishnan R, Lemke EA, Lindquist S, Deniz AA. A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidly fluctuating structures. Proceedings of the National Academy of Sciences of the United States of America. 104: 2649-54. PMID 17299036 DOI: 10.1073/pnas.0611503104  1
2006 Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, Rammner B, Gräter F, Hub JS, De Groot BL, et al. Molecular anatomy of a trafficking organelle. Cell. 127: 831-46. PMID 17110340 DOI: 10.1016/j.cell.2006.10.030  1
2005 Lemke EA, Klingauf J. Single synaptic vesicle tracking in individual hippocampal boutons at rest and during synaptic activity. The Journal of Neuroscience : the Official Journal of the Society For Neuroscience. 25: 11034-44. PMID 16306416 DOI: 10.1523/JNEUROSCI.2971-05.2005  1
2005 Jordan R, Lemke EA, Klingauf J. Visualization of synaptic vesicle movement in intact synaptic boutons using fluorescence fluctuation spectroscopy. Biophysical Journal. 89: 2091-102. PMID 15980175 DOI: 10.1529/biophysj.105.061663  1
2003 Chakraborty R, Lemke EA, Cao Z, Klebba PE, van der Helm D. Identification and mutational studies of conserved amino acids in the outer membrane receptor protein, FepA, which affect transport but not binding of ferric-enterobactin in Escherichia coli. Biometals : An International Journal On the Role of Metal Ions in Biology, Biochemistry, and Medicine. 16: 507-18. PMID 12779236 DOI: 10.1023/A:1023485632520  1
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