Peter F. Leadlay - Publications

Affiliations: 
Biochemistry University of Cambridge, Cambridge, England, United Kingdom 
Area:
Biosynthesis of Polyketide Antibiotics
Website:
http://www.bio.cam.ac.uk/~pflgroup/

214 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any innacuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2019 Hong H, Samborskyy M, Zhou Y, Leadlay PF. C-Nucleoside Formation in the Biosynthesis of the Antifungal Malayamycin A. Cell Chemical Biology. PMID 30713097 DOI: 10.1016/j.chembiol.2018.12.004  0.48
2018 Li S, Guo J, Reva A, Huang F, Xiong B, Liu Y, Deng Z, Leadlay PF, Sun Y. Methyltransferases of gentamicin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. PMID 29358400 DOI: 10.1073/pnas.1711603115  0.48
2017 Hong H, Samborskyy M, Usachova K, Schnatz K, Leadlay PF. Sulfation and amidinohydrolysis in the biosynthesis of giant linear polyenes. Beilstein Journal of Organic Chemistry. 13: 2408-2415. PMID 29234468 DOI: 10.3762/bjoc.13.238  0.48
2017 Bury PDS, Huang F, Li S, Sun Y, Leadlay PF, Dias MVB. Structural Basis of the Selectivity of GenN, an Aminoglycoside N-methyltransferase Involved in Gentamicin Biosynthesis. Acs Chemical Biology. PMID 28876898 DOI: 10.1021/acschembio.7b00466  0.48
2017 Xu W, Zhai G, Liu Y, Li Y, Shi Y, Hong K, Hong H, Leadlay PF, Deng Z, Sun Y. An Iterative Module in the Azalomycin F Polyketide Synthase Contains a Switchable Enoylreductase Domain. Angewandte Chemie (International Ed. in English). PMID 28418225 DOI: 10.1002/anie.201701220  0.48
2017 Yurkovich ME, Jenkins R, Sun Y, Tosin M, Leadlay PF. The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase. Chemical Communications (Cambridge, England). PMID 28124037 DOI: 10.1039/c6cc09934c  0.48
2017 Havemann J, Yurkovich ME, Jenkins R, Harringer S, Tao W, Wen S, Sun Y, Leadlay PF, Tosin M. Chemical probing of thiotetronate bio-assembly. Chemical Communications (Cambridge, England). PMID 28119973 DOI: 10.1039/c6cc09933e  0.48
2016 Hong H, Sun Y, Zhou Y, Stephens E, Samborskyy M, Leadlay PF. Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase. Beilstein Journal of Organic Chemistry. 12: 2164-2172. PMID 27829923 DOI: 10.3762/bjoc.12.206  0.48
2016 Parascandolo JS, Havemann J, Potter HK, Huang F, Riva E, Connolly J, Wilkening I, Song L, Leadlay PF, Tosin M. Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes. Angewandte Chemie (Weinheim An Der Bergstrasse, Germany). 128: 3524-3528. PMID 27478274 DOI: 10.1002/ange.201509038  0.48
2016 Murphy AC, Hong H, Vance S, Broadhurst RW, Leadlay PF. Broadening substrate specificity of a chain-extending ketosynthase through a single active-site mutation. Chemical Communications (Cambridge, England). PMID 27307197 DOI: 10.1039/c6cc03501a  1
2016 Netzker T, Schroeckh V, Gregory MA, Flak M, Krespach MK, Leadlay PF, Brakhage AA. An efficient method to generate gene deletion mutants of the rapamycin producing bacterium Streptomyces iranensis HM 35. Applied and Environmental Microbiology. PMID 27037115 DOI: 10.1128/AEM.00371-16  1
2016 Zabala D, Cartwright JW, Roberts DM, Law BJ, Song L, Samborskyy M, Leadlay PF, Micklefield J, Challis GL. A flavin-dependent decarboxylase-dehydrogenase-monooxygenase assembles the warhead of α, β-epoxyketone proteasome inhibitors. Journal of the American Chemical Society. PMID 26999044 DOI: 10.1021/jacs.6b01619  1
2016 Parascandolo JS, Havemann J, Potter HK, Huang F, Riva E, Connolly J, Wilkening I, Song L, Leadlay PF, Tosin M. Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes. Angewandte Chemie (International Ed. in English). PMID 26833898 DOI: 10.1002/anie.201509038  1
2015 Luhavaya H, Dias MV, Williams SR, Hong H, de Oliveira LG, Leadlay PF. Enzymology of Pyran Ring A Formation in Salinomycin Biosynthesis. Angewandte Chemie (Weinheim An Der Bergstrasse, Germany). 127: 13826-13829. PMID 27587902 DOI: 10.1002/ange.201507090  0.48
2015 Hong H, Samborskyy M, Lindner F, Leadlay PF. An Amidinohydrolase Provides the Missing Link in the Biosynthesis of Amino Marginolactone Antibiotics. Angewandte Chemie (International Ed. in English). PMID 26630438 DOI: 10.1002/anie.201509300  1
2015 Luhavaya H, Dias MV, Williams SR, Hong H, de Oliveira LG, Leadlay PF. Enzymology of Pyran Ring A Formation in Salinomycin Biosynthesis. Angewandte Chemie (International Ed. in English). PMID 26377145 DOI: 10.1002/anie.201507090  1
2015 Zhou Y, Murphy AC, Samborskyy M, Prediger P, Dias LC, Leadlay PF. Iterative Mechanism of Macrodiolide Formation in the Anticancer Compound Conglobatin. Chemistry & Biology. 22: 745-54. PMID 26091168 DOI: 10.1016/j.chembiol.2015.05.010  1
2015 Annaval T, Paris C, Leadlay PF, Jacob C, Weissman KJ. Evaluating Ketoreductase Exchanges as a Means of Rationally Altering Polyketide Stereochemistry. Chembiochem : a European Journal of Chemical Biology. 16: 1357-64. PMID 25851784 DOI: 10.1002/cbic.201500113  1
2015 Zhou Y, Prediger P, Dias LC, Murphy AC, Leadlay PF. Macrodiolide formation by the thioesterase of a modular polyketide synthase. Angewandte Chemie (International Ed. in English). 54: 5232-5. PMID 25753953 DOI: 10.1002/anie.201500401  1
2015 Huang C, Huang F, Moison E, Guo J, Jian X, Duan X, Deng Z, Leadlay PF, Sun Y. Delineating the biosynthesis of gentamicin x2, the common precursor of the gentamicin C antibiotic complex. Chemistry & Biology. 22: 251-61. PMID 25641167 DOI: 10.1016/j.chembiol.2014.12.012  1
2015 Tao W, Yurkovich ME, Wen S, Lebe KE, Samborskyy M, Liu Y, Yang A, Ju Y, Deng Z, Tosin M, Sun Y, Leadlay PF. A genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis Chemical Science. 7: 376-385. DOI: 10.1039/c5sc03059e  1
2014 Riva E, Wilkening I, Gazzola S, Li WM, Smith L, Leadlay PF, Tosin M. Chemical probes for the functionalization of polyketide intermediates. Angewandte Chemie (International Ed. in English). 53: 11944-9. PMID 25212788 DOI: 10.1002/anie.201407448  1
2014 Luhavaya H, Williams SR, Hong H, Gonzaga de Oliveira L, Leadlay PF. Site-specific modification of the anticancer and antituberculosis polyether salinomycin by biosynthetic engineering. Chembiochem : a European Journal of Chemical Biology. 15: 2081-5. PMID 25155178 DOI: 10.1002/cbic.201402300  1
2014 Leadlay PF. Structural biology: enzyme assembly line pictured. Nature. 510: 482-3. PMID 24965648 DOI: 10.1038/nature13505  1
2014 Vieweg L, Reichau S, Schobert R, Leadlay PF, Süssmuth RD. Recent advances in the field of bioactive tetronates. Natural Product Reports. 31: 1554-84. PMID 24965099 DOI: 10.1039/c4np00015c  1
2014 Hahn F, Kandziora N, Friedrich S, Leadlay PF. Synthesis of complex intermediates for the study of a dehydratase from borrelidin biosynthesis. Beilstein Journal of Organic Chemistry. 10: 634-40. PMID 24778714 DOI: 10.3762/bjoc.10.55  1
2014 Guo J, Huang F, Huang C, Duan X, Jian X, Leeper F, Deng Z, Leadlay PF, Sun Y. Specificity and promiscuity at the branch point in gentamicin biosynthesis. Chemistry & Biology. 21: 608-18. PMID 24746560 DOI: 10.1016/j.chembiol.2014.03.005  1
2014 Hüttel W, Spencer JB, Leadlay PF. Intermediates in monensin biosynthesis: A late step in biosynthesis of the polyether ionophore monensin is crucial for the integrity of cation binding. Beilstein Journal of Organic Chemistry. 10: 361-8. PMID 24605157 DOI: 10.3762/bjoc.10.34  1
2014 Kandziora N, Andexer JN, Moss SJ, Wilkinson B, Leadlay PF, Hahn F. Uncovering the origin of Z-configured double bonds in polyketides: Intermediate E-double bond formation during borrelidin biosynthesis Chemical Science. 5: 3563-3567. DOI: 10.1039/c4sc00883a  1
2013 Hong H, Fill T, Leadlay PF. A common origin for guanidinobutanoate starter units in antifungal natural products. Angewandte Chemie (International Ed. in English). 52: 13096-9. PMID 24218293 DOI: 10.1002/anie.201308136  1
2013 Porter JL, Tobias NJ, Pidot SJ, Falgner S, Tuck KL, Vettiger A, Hong H, Leadlay PF, Stinear TP. The cell wall-associated mycolactone polyketide synthases are necessary but not sufficient for mycolactone biosynthesis. Plos One. 8: e70520. PMID 23894666 DOI: 10.1371/journal.pone.0070520  1
2013 Kanchanabanca C, Tao W, Hong H, Liu Y, Hahn F, Samborskyy M, Deng Z, Sun Y, Leadlay PF. Unusual acetylation-elimination in the formation of tetronate antibiotics. Angewandte Chemie (International Ed. in English). 52: 5785-8. PMID 23606658 DOI: 10.1002/anie.201301680  1
2013 Guenin-Macé L, Veyron-Churlet R, Thoulouze MI, Romet-Lemonne G, Hong H, Leadlay PF, Danckaert A, Ruf MT, Mostowy S, Zurzolo C, Bousso P, Chrétien F, Carlier MF, Demangel C. Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation. The Journal of Clinical Investigation. 123: 1501-12. PMID 23549080 DOI: 10.1172/JCI66576  1
2012 Herrmann S, Siegl T, Luzhetska M, Petzke L, Jilg C, Welle E, Erb A, Leadlay PF, Bechthold A, Luzhetskyy A. Site-specific recombination strategies for engineering actinomycete genomes. Applied and Environmental Microbiology. 78: 1804-12. PMID 22247163 DOI: 10.1128/AEM.06054-11  1
2012 Yurkovich ME, Tyrakis PA, Hong H, Sun Y, Samborskyy M, Kamiya K, Leadlay PF. A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster. Chembiochem : a European Journal of Chemical Biology. 13: 66-71. PMID 22076845 DOI: 10.1002/cbic.201100590  1
2012 Moncrieffe MC, Fernandez MJ, Spiteller D, Matsumura H, Gay NJ, Luisi BF, Leadlay PF. Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII. Journal of Molecular Biology. 415: 92-101. PMID 22056329 DOI: 10.1016/j.jmb.2011.10.036  1
2011 Woolard J, Vousden W, Moss SJ, Krishnakumar A, Gammons MV, Nowak DG, Dixon N, Micklefield J, Spannhoff A, Bedford MT, Gregory MA, Martin CJ, Leadlay PF, Zhang MQ, Harper SJ, et al. Borrelidin modulates the alternative splicing of VEGF in favour of anti-angiogenic isoforms. Chemical Science (Royal Society of Chemistry : 2010). 2011: 273-278. PMID 22822423 DOI: 10.1039/C0SC00297F  1
2011 Tosin M, Smith L, Leadlay PF. Insights into lasalocid A ring formation by chemical chain termination in vivo. Angewandte Chemie (International Ed. in English). 50: 11930-3. PMID 22025396 DOI: 10.1002/anie.201106323  1
2011 Dickschat JS, Vergnolle O, Hong H, Garner S, Bidgood SR, Dooley HC, Deng Z, Leadlay PF, Sun Y. An additional dehydratase-like activity is required for lankacidin antibiotic biosynthesis. Chembiochem : a European Journal of Chemical Biology. 12: 2408-12. PMID 21953738 DOI: 10.1002/cbic.201100474  1
2011 Vergnolle O, Hahn F, Baerga-Ortiz A, Leadlay PF, Andexer JN. Stereoselectivity of isolated dehydratase domains of the borrelidin polyketide synthase: implications for cis double bond formation. Chembiochem : a European Journal of Chemical Biology. 12: 1011-4. PMID 21472957 DOI: 10.1002/cbic.201100011  1
2011 Andexer JN, Kendrew SG, Nur-e-Alam M, Lazos O, Foster TA, Zimmermann AS, Warneck TD, Suthar D, Coates NJ, Koehn FE, Skotnicki JS, Carter GT, Gregory MA, Martin CJ, Moss SJ, ... Leadlay PF, et al. Biosynthesis of the immunosuppressants FK506, FK520, and rapamycin involves a previously undescribed family of enzymes acting on chorismate. Proceedings of the National Academy of Sciences of the United States of America. 108: 4776-81. PMID 21383123 DOI: 10.1073/pnas.1015773108  1
2011 Kwan DH, Tosin M, Schläger N, Schulz F, Leadlay PF. Insights into the stereospecificity of ketoreduction in a modular polyketide synthase. Organic & Biomolecular Chemistry. 9: 2053-6. PMID 21340070 DOI: 10.1039/c1ob00022e  1
2011 Tosin M, Demydchuk Y, Parascandolo JS, Per CB, Leeper FJ, Leadlay PF. In vivo trapping of polyketide intermediates from an assembly line synthase using malonyl carba(dethia)-N-acetyl cysteamines. Chemical Communications (Cambridge, England). 47: 3460-2. PMID 21301753 DOI: 10.1039/c0cc05077f  1
2010 Magdevska V, Gaber R, Goranovič D, Kuščer E, Boakes S, Durán Alonso MB, Santamaría RI, Raspor P, Leadlay PF, Fujs S, Petković H. Robust reporter system based on chalcone synthase rppA gene from Saccharopolyspora erythraea. Journal of Microbiological Methods. 83: 111-9. PMID 20709115 DOI: 10.1016/j.mimet.2010.08.001  1
2010 Kwan DH, Leadlay PF. Mutagenesis of a modular polyketide synthase enoylreductase domain reveals insights into catalysis and stereospecificity. Acs Chemical Biology. 5: 829-38. PMID 20666435 DOI: 10.1021/cb100175a  1
2010 Dias MV, Huang F, Chirgadze DY, Tosin M, Spiteller D, Dry EF, Leadlay PF, Spencer JB, Blundell TL. Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK. The Journal of Biological Chemistry. 285: 22495-504. PMID 20430898 DOI: 10.1074/jbc.M110.107177  1
2010 Lazos O, Tosin M, Slusarczyk AL, Boakes S, Cortés J, Sidebottom PJ, Leadlay PF. Biosynthesis of the putative siderophore erythrochelin requires unprecedented crosstalk between separate nonribosomal peptide gene clusters. Chemistry & Biology. 17: 160-73. PMID 20189106 DOI: 10.1016/j.chembiol.2010.01.011  1
2010 Tosin M, Betancor L, Stephens E, Li WM, Spencer JB, Leadlay PF. Synthetic chain terminators off-load intermediates from a type I polyketide synthase. Chembiochem : a European Journal of Chemical Biology. 11: 539-46. PMID 20135665 DOI: 10.1002/cbic.200900772  1
2010 Sun Y, Hahn F, Demydchuk Y, Chettle J, Tosin M, Osada H, Leadlay PF. In vitro reconstruction of tetronate RK-682 biosynthesis. Nature Chemical Biology. 6: 99-101. PMID 20081823 DOI: 10.1038/nchembio.285  1
2009 Hong H, Leadlay PF, Staunton J. The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry. The Febs Journal. 276: 7057-69. PMID 19860832 DOI: 10.1111/j.1742-4658.2009.07418.x  1
2008 Smith L, Hong H, Spencer JB, Leadlay PF. Analysis of specific mutants in the lasalocid gene cluster: evidence for enzymatic catalysis of a disfavoured polyether ring closure. Chembiochem : a European Journal of Chemical Biology. 9: 2967-75. PMID 19025863 DOI: 10.1002/cbic.200800585  1
2008 Kwan DH, Sun Y, Schulz F, Hong H, Popovic B, Sim-Stark JC, Haydock SF, Leadlay PF. Prediction and manipulation of the stereochemistry of enoylreduction in modular polyketide synthases. Chemistry & Biology. 15: 1231-40. PMID 19022183 DOI: 10.1016/j.chembiol.2008.09.012  1
2008 Betancor L, Fernández MJ, Weissman KJ, Leadlay PF. Improved catalytic activity of a purified multienzyme from a modular polyketide synthase after coexpression with Streptomyces chaperonins in Escherichia coli. Chembiochem : a European Journal of Chemical Biology. 9: 2962-6. PMID 19021139 DOI: 10.1002/cbic.200800475  1
2008 Hong H, Coutanceau E, Leclerc M, Caleechurn L, Leadlay PF, Demangel C. Mycolactone diffuses from Mycobacterium ulcerans-infected tissues and targets mononuclear cells in peripheral blood and lymphoid organs. Plos Neglected Tropical Diseases. 2: e325. PMID 18941518 DOI: 10.1371/journal.pntd.0000325  1
2008 Kellenberger L, Galloway IS, Sauter G, Böhm G, Hanefeld U, Cortés J, Staunton J, Leadlay PF. A polylinker approach to reductive loop swaps in modular polyketide synthases. Chembiochem : a European Journal of Chemical Biology. 9: 2740-9. PMID 18937219 DOI: 10.1002/cbic.200800332  1
2008 Pidot SJ, Hong H, Seemann T, Porter JL, Yip MJ, Men A, Johnson M, Wilson P, Davies JK, Leadlay PF, Stinear TP. Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones. Bmc Genomics. 9: 462. PMID 18840298 DOI: 10.1186/1471-2164-9-462  1
2008 Schell U, Haydock SF, Kaja AL, Carletti I, Lill RE, Read E, Sheehan LS, Low L, Fernandez MJ, Grolle F, McArthur HA, Sheridan RM, Leadlay PF, Wilkinson B, Gaisser S. Engineered biosynthesis of hybrid macrolide polyketides containing D-angolosamine and D-mycaminose moieties. Organic & Biomolecular Chemistry. 6: 3315-27. PMID 18802638 DOI: 10.1039/b807914e  1
2008 Fan Q, Huang F, Leadlay PF, Spencer JB. The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: genetic and biochemical evidence for the roles of two glycosyltransferases and a deacetylase. Organic & Biomolecular Chemistry. 6: 3306-14. PMID 18802637 DOI: 10.1039/b808734b  1
2008 Leadlay P. Obituary: Jonathan B. Spencer (1960-2008) Chemistry & Biology. 15: 424-426. PMID 18551814 DOI: 10.1016/j.chembiol.2008.05.002  1
2008 Hong H, Demangel C, Pidot SJ, Leadlay PF, Stinear T. Mycolactones: immunosuppressive and cytotoxic polyketides produced by aquatic mycobacteria. Natural Product Reports. 25: 447-54. PMID 18497894 DOI: 10.1039/b803101k  1
2008 Truman AW, Fan Q, Röttgen M, Stegmann E, Leadlay PF, Spencer JB. The role of cep15 in the biosynthesis of chloroeremomycin: reactivation of an ancestral catalytic function. Chemistry & Biology. 15: 476-84. PMID 18482700 DOI: 10.1016/j.chembiol.2008.03.019  1
2008 Demydchuk Y, Sun Y, Hong H, Staunton J, Spencer JB, Leadlay PF. Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic. Chembiochem : a European Journal of Chemical Biology. 9: 1136-45. PMID 18404760 DOI: 10.1002/cbic.200700715  1
2008 Tran L, Tosin M, Spencer JB, Leadlay PF, Weissman KJ. Covalent linkage mediates communication between ACP and TE domains in modular polyketide synthases. Chembiochem : a European Journal of Chemical Biology. 9: 905-15. PMID 18348128 DOI: 10.1002/cbic.200700738  1
2008 te Poele EM, Samborskyy M, Oliynyk M, Leadlay PF, Bolhuis H, Dijkhuizen L. Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded. Plasmid. 59: 202-16. PMID 18295883 DOI: 10.1016/j.plasmid.2008.01.003  1
2008 Petkovi? H, Sandmann A, Challis IR, Hecht HJ, Silakowski B, Low L, Beeston N, Kuscer E, Garcia-Bernardo J, Leadlay PF, Kendrew SG, Wilkinson B, Müller R. Substrate specificity of the acyl transferase domains of EpoC from the epothilone polyketide synthase. Organic & Biomolecular Chemistry. 6: 500-6. PMID 18219420 DOI: 10.1039/b714804f  1
2008 Sun Y, Hong H, Gillies F, Spencer JB, Leadlay PF. Glyceryl-S-acyl carrier protein as an intermediate in the biosynthesis of tetronate antibiotics. Chembiochem : a European Journal of Chemical Biology. 9: 150-6. PMID 18046685 DOI: 10.1002/cbic.200700492  1
2007 Hong H, Stinear T, Porter J, Demangel C, Leadlay PF. A novel mycolactone toxin obtained by biosynthetic engineering. Chembiochem : a European Journal of Chemical Biology. 8: 2043-7. PMID 17907121 DOI: 10.1002/cbic.200700411  1
2007 Harvey BM, Mironenko T, Sun Y, Hong H, Deng Z, Leadlay PF, Weissman KJ, Haydock SF. Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137. Chemistry & Biology. 14: 703-14. PMID 17584617 DOI: 10.1016/j.chembiol.2007.05.011  1
2007 Oliynyk M, Samborskyy M, Lester JB, Mironenko T, Scott N, Dickens S, Haydock SF, Leadlay PF. Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338. Nature Biotechnology. 25: 447-53. PMID 17369815 DOI: 10.1038/nbt1297  1
2006 Sheehan LS, Lill RE, Wilkinson B, Sheridan RM, Vousden WA, Kaja AL, Crouse GD, Gifford J, Graupner PR, Karr L, Lewer P, Sparks TC, Leadlay PF, Waldron C, Martin CJ. Engineering of the spinosyn PKS: directing starter unit incorporation. Journal of Natural Products. 69: 1702-10. PMID 17190446 DOI: 10.1021/np0602517  1
2006 Sun Y, Hong H, Samborskyy M, Samboskyy M, Mironenko T, Leadlay PF, Haydock SF. Organization of the biosynthetic gene cluster in Streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin. Microbiology (Reading, England). 152: 3507-15. PMID 17159202 DOI: 10.1099/mic.0.29176-0  1
2006 Gregory MA, Hong H, Lill RE, Gaisser S, Petkovic H, Low L, Sheehan LS, Carletti I, Ready SJ, Ward MJ, Kaja AL, Weston AJ, Challis IR, Leadlay PF, Martin CJ, et al. Rapamycin biosynthesis: Elucidation of gene product function. Organic & Biomolecular Chemistry. 4: 3565-8. PMID 16990929 DOI: 10.1039/b608813a  1
2006 Wilkinson B, Gregory MA, Moss SJ, Carletti I, Sheridan RM, Kaja A, Ward M, Olano C, Mendez C, Salas JA, Leadlay PF, vanGinckel R, Zhang MQ. Separation of anti-angiogenic and cytotoxic activities of borrelidin by modification at the C17 side chain. Bioorganic & Medicinal Chemistry Letters. 16: 5814-7. PMID 16962775 DOI: 10.1016/j.bmcl.2006.08.073  1
2006 Harvey BM, Hong H, Jones MA, Hughes-Thomas ZA, Goss RM, Heathcote ML, Bolanos-Garcia VM, Kroutil W, Staunton J, Leadlay PF, Spencer JB. Evidence that a novel thioesterase is responsible for polyketide chain release during biosynthesis of the polyether ionophore monensin. Chembiochem : a European Journal of Chemical Biology. 7: 1435-42. PMID 16897798 DOI: 10.1002/cbic.200500474  1
2006 Moss SJ, Carletti I, Olano C, Sheridan RM, Ward M, Math V, Nur-E-Alam M, Braña AF, Zhang MQ, Leadlay PF, Méndez C, Salas JA, Wilkinson B. Biosynthesis of the angiogenesis inhibitor borrelidin: directed biosynthesis of novel analogues. Chemical Communications (Cambridge, England). 2341-3. PMID 16733573 DOI: 10.1039/b602931k  1
2006 Huang F, Haydock SF, Spiteller D, Mironenko T, Li TL, O'Hagan D, Leadlay PF, Spencer JB. The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A. Chemistry & Biology. 13: 475-84. PMID 16720268 DOI: 10.1016/j.chembiol.2006.02.014  1
2006 O'Hare HM, Baerga-Ortiz A, Popovic B, Spencer JB, Leadlay PF. High-throughput mutagenesis to evaluate models of stereochemical control in ketoreductase domains from the erythromycin polyketide synthase. Chemistry & Biology. 13: 287-96. PMID 16638534 DOI: 10.1016/j.chembiol.2006.01.003  1
2006 Baerga-Ortiz A, Popovic B, Siskos AP, O'Hare HM, Spiteller D, Williams MG, Campillo N, Spencer JB, Leadlay PF. Directed mutagenesis alters the stereochemistry of catalysis by isolated ketoreductase domains from the erythromycin polyketide synthase. Chemistry & Biology. 13: 277-85. PMID 16638533 DOI: 10.1016/j.chembiol.2006.01.004  1
2006 Gallimore AR, Stark CB, Bhatt A, Harvey BM, Demydchuk Y, Bolanos-Garcia V, Fowler DJ, Staunton J, Leadlay PF, Spencer JB. Evidence for the role of the monB genes in polyether ring formation during monensin biosynthesis. Chemistry & Biology. 13: 453-60. PMID 16632258 DOI: 10.1016/j.chembiol.2006.01.013  1
2005 Weissman KJ, Leadlay PF. Combinatorial biosynthesis of reduced polyketides. Nature Reviews. Microbiology. 3: 925-36. PMID 16322741 DOI: 10.1038/nrmicro1287  1
2005 Siskos AP, Baerga-Ortiz A, Bali S, Stein V, Mamdani H, Spiteller D, Popovic B, Spencer JB, Staunton J, Weissman KJ, Leadlay PF. Molecular basis of Celmer's rules: stereochemistry of catalysis by isolated ketoreductase domains from modular polyketide synthases. Chemistry & Biology. 12: 1145-53. PMID 16242657 DOI: 10.1016/j.chembiol.2005.08.017  1
2005 Bhatt A, Stark CB, Harvey BM, Gallimore AR, Demydchuk YA, Spencer JB, Staunton J, Leadlay PF. Accumulation of an E,E,E-triene by the monensin-producing polyketide synthase when oxidative cyclization is blocked. Angewandte Chemie (International Ed. in English). 44: 7075-8. PMID 16217816 DOI: 10.1002/anie.200501757  1
2005 Haydock SF, Appleyard AN, Mironenko T, Lester J, Scott N, Leadlay PF. Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449. Microbiology (Reading, England). 151: 3161-9. PMID 16207901 DOI: 10.1099/mic.0.28194-0  1
2005 Hong H, Stinear T, Skelton P, Spencer JB, Leadlay PF. Structure elucidation of a novel family of mycolactone toxins from the frog pathogen Mycobacterium sp. MU128FXT by mass spectrometry. Chemical Communications (Cambridge, England). 4306-8. PMID 16113730 DOI: 10.1039/b506835e  1
2005 Gregory MA, Petkovic H, Lill RE, Moss SJ, Wilkinson B, Gaisser S, Leadlay PF, Sheridan RM. Mutasynthesis of rapamycin analogues through the manipulation of a gene governing starter unit biosynthesis. Angewandte Chemie (International Ed. in English). 44: 4757-60. PMID 15977283 DOI: 10.1002/anie.200462784  1
2005 Hong H, Appleyard AN, Siskos AP, Garcia-Bernardo J, Staunton J, Leadlay PF. Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry. The Febs Journal. 272: 2373-87. PMID 15885088 DOI: 10.1111/j.1742-4658.2005.04615.x  1
2005 Hong H, Spencer JB, Porter JL, Leadlay PF, Stinear T. A novel mycolactone from a clinical isolate of Mycobacterium ulcerans provides evidence for additional toxin heterogeneity as a result of specific changes in the modular polyketide synthase. Chembiochem : a European Journal of Chemical Biology. 6: 643-8. PMID 15750996 DOI: 10.1002/cbic.200400339  1
2005 Stinear TP, Hong H, Frigui W, Pryor MJ, Brosch R, Garnier T, Leadlay PF, Cole ST. Common evolutionary origin for the unstable virulence plasmid pMUM found in geographically diverse strains of Mycobacterium ulcerans. Journal of Bacteriology. 187: 1668-76. PMID 15716437 DOI: 10.1128/JB.187.5.1668-1676.2005  1
2004 Boakes S, Oliynyk M, Cortés J, Böhm I, Rudd BA, Revill WP, Staunton J, Leadlay PF. A new modular polyketide synthase in the erythromycin producer Saccharopolyspora erythraea. Journal of Molecular Microbiology and Biotechnology. 8: 73-80. PMID 15925898 DOI: 10.1159/000084562  1
2004 Haydock SF, Mironenko T, Ghoorahoo HI, Leadlay PF. The putative elaiophylin biosynthetic gene cluster in Streptomyces sp. DSM4137 is adjacent to genes encoding adenosylcobalamin-dependent methylmalonyl CoA mutase and to genes for synthesis of cobalamin. Journal of Biotechnology. 113: 55-68. PMID 15380647 DOI: 10.1016/j.jbiotec.2004.03.022  1
2004 Garcia-Bernardo J, Xiang L, Hong H, Moore BS, Leadlay PF. Engineered biosynthesis of phenyl-substituted polyketides. Chembiochem : a European Journal of Chemical Biology. 5: 1129-31. PMID 15300838 DOI: 10.1002/cbic.200400007  1
2004 Olano C, Moss SJ, Braña AF, Sheridan RM, Math V, Weston AJ, Méndez C, Leadlay PF, Wilkinson B, Salas JA. Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tü4055: insights into nitrile formation. Molecular Microbiology. 52: 1745-56. PMID 15186422 DOI: 10.1111/j.1365-2958.2004.04090.x  1
2004 Lowden PA, Böhm GA, Metcalfe S, Staunton J, Leadlay PF. New rapamycin derivatives by precursor-directed biosynthesis. Chembiochem : a European Journal of Chemical Biology. 5: 535-8. PMID 15185378 DOI: 10.1002/cbic.200300758  1
2004 Gregory MA, Gaisser S, Lill RE, Hong H, Sheridan RM, Wilkinson B, Petkovic H, Weston AJ, Carletti I, Lee HL, Staunton J, Leadlay PF. Isolation and characterization of pre-rapamycin, the first macrocyclic intermediate in the biosynthesis of the immunosuppressant rapamycin by S. hygroscopicus. Angewandte Chemie (International Ed. in English). 43: 2551-3. PMID 15127450 DOI: 10.1002/anie.200453764  1
2004 Li TL, Huang F, Haydock SF, Mironenko T, Leadlay PF, Spencer JB. Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase. Chemistry & Biology. 11: 107-19. PMID 15113000 DOI: 10.1016/j.chembiol.2004.01.001  1
2004 Olano C, Wilkinson B, Sánchez C, Moss SJ, Sheridan R, Math V, Weston AJ, Braña AF, Martin CJ, Oliynyk M, Méndez C, Leadlay PF, Salas JA. Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tü4055: cluster analysis and assignment of functions. Chemistry & Biology. 11: 87-97. PMID 15112998 DOI: 10.1016/j.chembiol.2003.12.018  1
2004 Stinear TP, Mve-Obiang A, Small PL, Frigui W, Pryor MJ, Brosch R, Jenkin GA, Johnson PD, Davies JK, Lee RE, Adusumilli S, Garnier T, Haydock SF, Leadlay PF, Cole ST. Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans. Proceedings of the National Academy of Sciences of the United States of America. 101: 1345-9. PMID 14736915 DOI: 10.1073/pnas.0305877101  1
2004 Weissman KJ, Hong H, Oliynyk M, Siskos AP, Leadlay PF. Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraea. Chembiochem : a European Journal of Chemical Biology. 5: 116-25. PMID 14695521 DOI: 10.1002/cbic.200300775  1
2003 Martin CJ, Timoney MC, Sheridan RM, Kendrew SG, Wilkinson B, Staunton JC, Leadlay PF. Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase. Organic & Biomolecular Chemistry. 1: 4144-7. PMID 14685317 DOI: 10.1039/b310740j  1
2003 Hong H, Gates PJ, Staunton J, Stinear T, Cole ST, Leadlay PF, Spencer JB. Identification using LC-MSn of co-metabolites in the biosynthesis of the polyketide toxin mycolactone by a clinical isolate of Mycobacterium ulcerans. Chemical Communications (Cambridge, England). 2822-3. PMID 14651122  1
2003 Olano C, Wilkinson B, Moss SJ, Braña AF, Méndez C, Leadlay PF, Salas JA. Evidence from engineered gene fusions for the repeated use of a module in a modular polyketide synthase. Chemical Communications (Cambridge, England). 2780-2. PMID 14651102  1
2003 Squire CM, Goss RJ, Hong H, Leadlay PF, Staunton J. Catalytically active tetramodular 6-deoxyerythonolide B synthase fusion proteins. Chembiochem : a European Journal of Chemical Biology. 4: 1225-8. PMID 14613116 DOI: 10.1002/cbic.200300671  1
2003 Hughes-Thomas ZA, Stark CB, Böhm IU, Staunton J, Leadlay PF. Intermediates released from a polyether-producing polyketide synthase provide insight into the mechanism of oxidative cyclization. Angewandte Chemie (International Ed. in English). 42: 4475-8. PMID 14520742 DOI: 10.1002/anie.200351375  1
2003 Gaisser S, Kellenberger L, Kaja AL, Weston AJ, Lill RE, Wirtz G, Kendrew SG, Low L, Sheridan RM, Wilkinson B, Galloway IS, Stutzman-Engwall K, McArthur HA, Staunton J, Leadlay PF. Direct production of ivermectin-like drugs after domain exchange in the avermectin polyketide synthase of Streptomyces avermitilis ATCC31272. Organic & Biomolecular Chemistry. 1: 2840-7. PMID 12968333 DOI: 10.1039/b304022d  1
2003 Broadhurst RW, Nietlispach D, Wheatcroft MP, Leadlay PF, Weissman KJ. The structure of docking domains in modular polyketide synthases. Chemistry & Biology. 10: 723-31. PMID 12954331 DOI: 10.1016/S1074-5521(03)00156-X  1
2003 Oliynyk M, Stark CB, Bhatt A, Jones MA, Hughes-Thomas ZA, Wilkinson C, Oliynyk Z, Demydchuk Y, Staunton J, Leadlay PF. Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization. Molecular Microbiology. 49: 1179-90. PMID 12940979 DOI: 10.1046/j.1365-2958.2003.03571.x  1
2003 Petkovic H, Lill RE, Sheridan RM, Wilkinson B, McCormick EL, McArthur HA, Staunton J, Leadlay PF, Kendrew SG. A novel erythromycin, 6-desmethyl erythromycin D, made by substituting an acyltransferase domain of the erythromycin polyketide synthase. The Journal of Antibiotics. 56: 543-51. PMID 12931864  1
2003 Del Vecchio F, Petkovic H, Kendrew SG, Low L, Wilkinson B, Lill R, Cortés J, Rudd BA, Staunton J, Leadlay PF. Active-site residue, domain and module swaps in modular polyketide synthases. Journal of Industrial Microbiology & Biotechnology. 30: 489-94. PMID 12811585 DOI: 10.1007/s10295-003-0062-0  1
2003 Leadlay P, Baerga-Ortiz A. Mammalian fatty acid synthase: closure on a textbook mechanism? Chemistry & Biology. 10: 101-3. PMID 12618179 DOI: 10.1016/S1074-5521(03)00029-2  1
2003 Wilkinson B, Kendrew SG, Sheridan RM, Leadlay PF. Biosynthetic engineering of polyketide synthases Expert Opinion On Therapeutic Patents. 13: 1579-1606. DOI: 10.1517/13543776.13.10.1579  1
2002 Thomas I, Martin CJ, Wilkinson CJ, Staunton J, Leadlay PF. Skipping in a hybrid polyketide synthase. Evidence for ACP-to-ACP chain transfer. Chemistry & Biology. 9: 781-7. PMID 12144921 DOI: 10.1016/S1074-5521(02)00164-3  1
2002 Wilkinson CJ, Hughes-Thomas ZA, Martin CJ, Böhm I, Mironenko T, Deacon M, Wheatcroft M, Wirtz G, Staunton J, Leadlay PF. Increasing the efficiency of heterologous promoters in actinomycetes. Journal of Molecular Microbiology and Biotechnology. 4: 417-26. PMID 12125822  1
2002 Gaisser S, Martin CJ, Wilkinson B, Sheridan RM, Lill RE, Weston AJ, Ready SJ, Waldron C, Crouse GD, Leadlay PF, Staunton J. Engineered biosynthesis of novel spinosyns bearing altered deoxyhexose substituents. Chemical Communications (Cambridge, England). 618-9. PMID 12120151  1
2002 Gaisser S, Lill R, Staunton J, Méndez C, Salas J, Leadlay PF. Parallel pathways for oxidation of 14-membered polyketide macrolactones in Saccharopolyspora erythraea. Molecular Microbiology. 44: 771-81. PMID 11994157 DOI: 10.1046/j.1365-2958.2002.02910.x  1
2002 Long PF, Wilkinson CJ, Bisang CP, Cortés J, Dunster N, Oliynyk M, McCormick E, McArthur H, Mendez C, Salas JA, Staunton J, Leadlay PF. Engineering specificity of starter unit selection by the erythromycin-producing polyketide synthase. Molecular Microbiology. 43: 1215-25. PMID 11918808 DOI: 10.1046/j.1365-2958.2002.02815.x  1
2002 Østergaard LH, Kellenberger L, Cortés J, Roddis MP, Deacon M, Staunton J, Leadlay PF. Stereochemistry of catalysis by the ketoreductase activity in the first extension module of the erythromycin polyketide synthase. Biochemistry. 41: 2719-26. PMID 11851419 DOI: 10.1021/bi0117605  1
2002 Weitnauer G, Gaisser S, Kellenberger L, Leadlay PF, Bechthold A. Analysis of a C-methyltransferase gene (aviG1) involved in avilamycin biosynthesis in Streptomyces viridochromogenes Tü57 and complementation of a Saccharopolyspora erythraea eryBIII mutant by aviG1. Microbiology (Reading, England). 148: 373-9. PMID 11832501  1
2002 Hong H, Østergaard L, Heathcote ML, Staunton J, Leadlay PF. Investigation of malfunction in an engineered polyketide synthase by proteolysis and LC-MS Proceedings 50th Asms Conference On Mass Spectrometry and Allied Topics. 355-356.  1
2001 Leadlay PF, Staunton J, Oliynyk M, Bisang C, Cortés J, Frost E, Hughes-Thomas ZA, Jones MA, Kendrew SG, Lester JB, Long PF, McArthur HA, McCormick EL, Oliynyk Z, Stark CB, et al. Engineering of complex polyketide biosynthesis--insights from sequencing of the monensin biosynthetic gene cluster. Journal of Industrial Microbiology & Biotechnology. 27: 360-7. PMID 11774001 DOI: 10.1038/sj/jim/7000204  1
2001 Wilkinson CJ, Frost EJ, Staunton J, Leadlay PF. Chain initiation on the soraphen-producing modular polyketide synthase from Sorangium cellulosum. Chemistry & Biology. 8: 1197-208. PMID 11755398 DOI: 10.1016/S1074-5521(01)00087-4  1
2001 Gaisser S, Lill R, Wirtz G, Grolle F, Staunton J, Leadlay PF. New erythromycin derivatives from Saccharopolyspora erythraea using sugar O-methyltransferases from the spinosyn biosynthetic gene cluster. Molecular Microbiology. 41: 1223-31. PMID 11555300 DOI: 10.1046/j.1365-2958.2001.02594.x  1
2001 Rowe CJ, Böhm IU, Thomas IP, Wilkinson B, Rudd BA, Foster G, Blackaby AP, Sidebottom PJ, Roddis Y, Buss AD, Staunton J, Leadlay PF. Engineering a polyketide with a longer chain by insertion of an extra module into the erythromycin-producing polyketide synthase. Chemistry & Biology. 8: 475-85. PMID 11358694 DOI: 10.1016/S1074-5521(01)00024-2  1
2001 Holzbaur IE, Ranganathan A, Thomas IP, Kearney DJ, Reather JA, Rudd BA, Staunton J, Leadlay PF. Molecular basis of Celmer's rules: role of the ketosynthase domain in epimerisation and demonstration that ketoreductase domains can have altered product specificity with unnatural substrates. Chemistry & Biology. 8: 329-40. PMID 11325589 DOI: 10.1016/S1074-5521(01)00014-X  1
2001 Heathcote ML, Staunton J, Leadlay PF. Role of type II thioesterases: evidence for removal of short acyl chains produced by aberrant decarboxylation of chain extender units. Chemistry & Biology. 8: 207-20. PMID 11251294 DOI: 10.1016/S1074-5521(01)00002-3  1
2001 Lowden PA, Wilkinson B, Böhm GA, Handa S, Floss HG, Leadlay PF, Staunton J. Origin and True Nature of the Starter Unit for the Rapamycin Polyketide Synthase We thank Dr. Bradley S. Moore for help with the deuterium NMR experiments. This work was supported by grants from The Wellcome Trust (to J.S. and P.F.L.) and from the NIH (AI20264 to H.G.F.). Angewandte Chemie (International Ed. in English). 40: 777-779. PMID 11241621 DOI: 10.1002/1521-3773(20010216)40:4<777::AID-ANIE7770>3.0.CO;2-9  1
2000 Thomä NH, Evans PR, Leadlay PF. Protection of radical intermediates at the active site of adenosylcobalamin-dependent methylmalonyl-CoA mutase. Biochemistry. 39: 9213-21. PMID 10924114 DOI: 10.1021/bi0004302  1
2000 Gaisser S, Reather J, Wirtz G, Kellenberger L, Staunton J, Leadlay PF. A defined system for hybrid macrolide biosynthesis in Saccharopolyspora erythraea. Molecular Microbiology. 36: 391-401. PMID 10792725 DOI: 10.1046/j.1365-2958.2000.01856.x  1
2000 Wilkinson B, Foster G, Rudd BA, Taylor NL, Blackaby AP, Sidebottom PJ, Cooper DJ, Dawson MJ, Buss AD, Gaisser S, Böhm IU, Rowe CJ, Cortés J, Leadlay PF, Staunton J. Novel octaketide macrolides related to 6-deoxyerythronolide B provide evidence for iterative operation of the erythromycin polyketide synthase. Chemistry & Biology. 7: 111-7. PMID 10662692 DOI: 10.1016/S1074-5521(00)00076-4  1
2000 Bycroft M, Weissman KJ, Staunton J, Leadlay PF. Efficient purification and kinetic characterization of a bimodular derivative of the erythromycin polyketide synthase. European Journal of Biochemistry / Febs. 267: 520-6. PMID 10632721 DOI: 10.1046/j.1432-1327.2000.01025.x  1
1999 Weissman KJ, Kearney GC, Leadlay PF, Staunton J. Structural elucidation studies of polyketide tetrasubstituted delta-lactones by gas chromatography/tandem mass spectrometry and electrospray mass spectrometry Rapid Communications in Mass Spectrometry : Rcm. 13: 2103-8. PMID 10523767 DOI: 10.1002/(SICI)1097-0231(19991115)13:21<2103::AID-RCM760>3.0.CO;2-Y  1
1999 Bisang C, Long PF, Cortés J, Westcott J, Crosby J, Matharu AL, Cox RJ, Simpson TJ, Staunton J, Leadlay PF. A chain initiation factor common to both modular and aromatic polyketide synthases. Nature. 401: 502-5. PMID 10519556 DOI: 10.1038/46829  1
1999 Ranganathan A, Timoney M, Bycroft M, Cortés J, Thomas IP, Wilkinson B, Kellenberger L, Hanefeld U, Galloway IS, Staunton J, Leadlay PF. Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: a route to simple statin analogues. Chemistry & Biology. 6: 731-41. PMID 10508677 DOI: 10.1016/S1074-5521(00)80020-4  1
1999 Kearney GC, Gates PJ, Leadlay PF, Staunton J, Jones R. Structural elucidation studies of erythromycins by electrospray tandem mass spectrometry II. Rapid Communications in Mass Spectrometry : Rcm. 13: 1650-6. PMID 10440983 DOI: 10.1002/(SICI)1097-0231(19990830)13:16<1650::AID-RCM693>3.0.CO;2-8  1
1999 Holzbaur IE, Harris RC, Bycroft M, Cortes J, Bisang C, Staunton J, Rudd BA, Leadlay PF. Molecular basis of Celmer's rules: the role of two ketoreductase domains in the control of chirality by the erythromycin modular polyketide synthase. Chemistry & Biology. 6: 189-95. PMID 10099131 DOI: 10.1016/S1074-5521(99)80035-0  1
1999 Gates PJ, Kearney GC, Jones R, Leadlay PF, Staunton J. Structural elucidation studies of erythromycins by electrospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry : Rcm. 13: 242-6. PMID 10097401 DOI: 10.1002/(SICI)1097-0231(19990228)13:4<242::AID-RCM447>3.0.CO;2-B  1
1999 Mirjalili N, Zormpaidis V, Leadlay PF, Ison AP. The effect of rapeseed oil uptake on the production of erythromycin and triketide lactone by Saccharopolyspora erythraea Biotechnology Progress. 15: 911-918. DOI: 10.1021/bp990099h  1
1999 Large KP, Mirjalili N, Peacock MOLM, Zormpaidis V, Walsh M, Cavanagh ME, Leadlay PF, Ison AP. Lipase activity in Streptomycetes Enzyme and Microbial Technology. 25: 569-575. DOI: 10.1016/S0141-0229(99)00080-0  1
1998 Pacey MS, Dirlam JP, Geldart RW, Leadlay PF, McArthur HA, McCormick EL, Monday RA, O'Connell TN, Staunton J, Winchester TJ. Novel erythromycins from a recombinant Saccharopolyspora erythraea strain NRRL 2338 pIG1. I. Fermentation, isolation and biological activity. The Journal of Antibiotics. 51: 1029-34. PMID 9918396  1
1998 Weissman KJ, Bycroft M, Cutter AL, Hanefeld U, Frost EJ, Timoney MC, Harris R, Handa S, Roddis M, Staunton J, Leadlay PF. Evaluating precursor-directed biosynthesis towards novel erythromycins through in vitro studies on a bimodular polyketide synthase. Chemistry & Biology. 5: 743-54. PMID 9862800  1
1998 Thomä NH, Meier TW, Evans PR, Leadlay PF. Stabilization of radical intermediates by an active-site tyrosine residue in methylmalonyl-CoA mutase. Biochemistry. 37: 14386-93. PMID 9772164 DOI: 10.1021/bi981375o  1
1998 Thoma NH, Leadlay PF. 665th Meeting, University of Southampton, United Kingdom, 31 March - 2 April 1998: Structure and mechanism of enzymes: Mechanistic and structural studies on methylmalonyl-CoA mutase Biochemical Society Transactions. 26: 293-298. PMID 9765867  1
1998 Celis RTF, Leadlay PF, Roy I, Hansen A. Phosphorylation of the periplasmic binding protein in two transport systems for arginine incorporation in Escherichia coli K-12 is unrelated to the function of the transport system Journal of Bacteriology. 180: 4828-4833. PMID 9733684  1
1998 Rowe CJ, Cortés J, Gaisser S, Staunton J, Leadlay PF. Construction of new vectors for high-level expression in actinomycetes. Gene. 216: 215-23. PMID 9714812 DOI: 10.1016/S0378-1119(98)00327-8  1
1998 Böhm I, Holzbaur IE, Hanefeld U, Cortés J, Staunton J, Leadlay PF. Engineering of a minimal modular polyketide synthase, and targeted alteration of the stereospecificity of polyketide chain extension. Chemistry & Biology. 5: 407-12. PMID 9710562  1
1998 Weissman KJ, Bycroft M, Staunton J, Leadlay PF. Origin of starter units for erythromycin biosynthesis. Biochemistry. 37: 11012-7. PMID 9692995 DOI: 10.1021/bi9806246  1
1998 Gaisser S, Böhm GA, Doumith M, Raynal MC, Dhillon N, Cortés J, Leadlay PF. Analysis of eryBI, eryBIII and eryBVII from the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea Molecular and General Genetics. 258: 78-88. PMID 9613575 DOI: 10.1007/s004380050709  1
1998 Salah-Bey K, Doumith M, Michel JM, Haydock S, Cortés J, Leadlay PF, Raynal MC. Targeted gene inactivation for the elucidation of deoxysugar biosynthesis in the erythromycin producer Saccharopolyspora erythraea Molecular and General Genetics. 257: 542-553. PMID 9563840 DOI: 10.1007/s004380050680  1
1998 Khaw LE, Böhm GA, Metcalfe S, Staunton J, Leadlay PF. Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase. Journal of Bacteriology. 180: 809-14. PMID 9473033  1
1998 Gaisser S, Leadlay PF. Sugaring the pill by design Nature Biotechnology. 16: 19-20. PMID 9447583 DOI: 10.1038/nbt0198-19  1
1998 Marsden AF, Wilkinson B, Cortés J, Dunster NJ, Staunton J, Leadlay PF. Engineering broader specificity into an antibiotic-producing polyketide synthase. Science (New York, N.Y.). 279: 199-202. PMID 9422686 DOI: 10.1126/science.279.5348.199  1
1998 Wilkinson AL, Hanefeld U, Wilkinson B, Leadlay PF, Staunton J. Enantiospecific synthesis of tetrasubstituted δ-lactones Tetrahedron Letters. 39: 9827-9830. DOI: 10.1016/S0040-4039(98)02243-6  1
1998 Weissman KJ, Smith CJ, Hanefeld U, Aggarwal R, Bycroft M, Staunton J, Leadlay PF. The thioesterase of the erythromycin-producing polyketide synthase: Influence of acyl chain structure on the mode of release of substrate analogues from the acyl enzyme intermediates Angewandte Chemie - International Edition. 37: 1437-1440. DOI: 10.1002/(SICI)1521-3773(19980605)37:10<1437::AID-ANIE1437>3.0.CO;2-7  1
1997 Leadlay PF. Combinatorial approaches to polyketide biosynthesis Current Opinion in Chemical Biology. 1: 162-168. PMID 9734993  1
1997 Gaisser S, Böhm GA, Cortés J, Leadlay PF. Analysis of seven genes from the eryAI-eryK region of the erythromycin biosynthetic: Gene cluster in Saccharopolyspora erythraea Molecular and General Genetics. 256: 239-251. PMID 9393448 DOI: 10.1007/s004380050566  1
1997 Weissman KJ, Timoney M, Bycroft M, Grice P, Hanefeld U, Staunton J, Leadlay PF. The molecular basis of Celmer's rules: the stereochemistry of the condensation step in chain extension on the erythromycin polyketide synthase. Biochemistry. 36: 13849-55. PMID 9374862 DOI: 10.1021/bi971566b  1
1997 König A, Schwecke T, Molnar I, Böhm GA, Lowden PA, Staunton J, Leadlay PF. The pipecolate-incorporating enzyme for the biosynthesis of the immunosuppressant rapamycin--nucleotide sequence analysis, disruption and heterologous expression of rapP from Streptomyces hygroscopicus. European Journal of Biochemistry / Febs. 247: 526-34. PMID 9266694  1
1997 Lewis HD, Floriam B, Linton KI, White J, Bibb MJ, Hunter LS, Leadlay PF. Evidence for a novel mechanism of resistance to the polyether polyketide tetronasin Faseb Journal. 11: A1032.  1
1997 Galloway IS, Rowe CJ, Cortes J, Staunton J, Leadlay PF. A system for the enhanced expression of genes in saccharopolyspora erythraea and its use in the production of novel polyketides Faseb Journal. 11: A1144.  1
1997 Leadlay PF. Structure and mechanism of modular polyketide synthases Faseb Journal. 11: A1291.  1
1996 Oliynyk M, Brown MJ, Cortés J, Staunton J, Leadlay PF. A hybrid modular polyketide synthase obtained by domain swapping. Chemistry & Biology. 3: 833-9. PMID 8939702  1
1996 Thomä NH, Leadlay PF. Homology modeling of human methylmalonyl-CoA mutase: A structural basis for point mutations causing methylmalonic aciduria Protein Science. 5: 1922-1927. PMID 8880917  1
1996 Mancia F, Keep NH, Nakagawa A, Leadlay PF, McSweeney S, Rasmussen B, Bösecke P, Diat O, Evans PR. How coenzyme B12 radicals are generated: the crystal structure of methylmalonyl-coenzyme A mutase at 2 A resolution. Structure (London, England : 1993). 4: 339-50. PMID 8805541  1
1996 Meier TW, Thomä NH, Leadlay PF. Tritium isotope effects in adenosylcobalamin-dependent methylmalonyl-CoA mutase. Biochemistry. 35: 11791-6. PMID 8794760 DOI: 10.1021/bi961250o  1
1996 Aparicio JF, Molnár I, Schwecke T, König A, Haydock SF, Khaw LE, Staunton J, Leadlay PF. Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of the enzymatic domains in the modular polyketide synthase. Gene. 169: 9-16. PMID 8635756 DOI: 10.1016/0378-1119(95)00800-4  1
1996 Molnár I, Aparicio JF, Haydock SF, Khaw LE, Schwecke T, König A, Staunton J, Leadlay PF. Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase. Gene. 169: 1-7. PMID 8635730 DOI: 10.1016/0378-1119(95)00799-7  1
1996 Staunton J, Caffrey P, Aparicio JF, Roberts GA, Bethell SS, Leadlay PF. Evidence for a double-helical structure for modular polyketide synthases. Nature Structural Biology. 3: 188-92. PMID 8564546 DOI: 10.1038/nsb0296-188  1
1996 Less SL, Leadlay PF, Dutton CJ, Staunton J. Biosynthesis of tetronasin: Part 7. Preparation of structural analogues of the tetraketide biosynthetic precursor to tetronasin Tetrahedron Letters. 37: 3519-3520. DOI: 10.1016/0040-4039(96)00601-6  1
1996 Less SL, Handa S, Millburn K, Leadlay PF, Dutton CJ, Staunton J. Biosynthesis of tetronasin: Part 6. Preparation of structural analogues of the diketide and triketide biosynthetic precursors to tetronasin Tetrahedron Letters. 37: 3515-3518. DOI: 10.1016/0040-4039(96)00600-4  1
1996 Less SL, Handa S, Leadlay PF, Dutton CJ, Staunton J. Biosynthesis of tetronasin: Part 5. Novel fluorinated and non-fluorinated analogues of tetronasin via intact incorporation of di-, tri- and tetraketide analogue precursors Tetrahedron Letters. 37: 3511-3514. DOI: 10.1016/0040-4039(96)00599-0  1
1996 Lowden PAS, Böhm GA, Staunton J, Leadlay PF. The nature of the starter unit for the rapamycin polyketide synthase Angewandte Chemie (International Edition in English). 35: 2249-2251.  1
1995 Wiesmann KE, Cortés J, Brown MJ, Cutter AL, Staunton J, Leadlay PF. Polyketide synthesis in vitro on a modular polyketide synthase. Chemistry & Biology. 2: 583-9. PMID 9383462 DOI: 10.1016/1074-5521(95)90122-1  1
1995 Cortes J, Wiesmann KE, Roberts GA, Brown MJ, Staunton J, Leadlay PF. Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage. Science (New York, N.Y.). 268: 1487-9. PMID 7770773  1
1995 Schwecke T, Aparicio JF, Molnár I, König A, Khaw LE, Haydock SF, Oliynyk M, Caffrey P, Cortés J, Lester JB, Böhm GA, Staunton J, Leadlay PF. The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin Proceedings of the National Academy of Sciences of the United States of America. 92: 7839-7843. PMID 7644502 DOI: 10.1073/pnas.92.17.7839  1
1995 Haydock SF, Aparicio JF, Molnár I, Schwecke T, Khaw LE, König A, Marsden AF, Galloway IS, Staunton J, Leadlay PF. Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA:acyl carrier protein transacylase domains in modular polyketide synthases. Febs Letters. 374: 246-8. PMID 7589545 DOI: 10.1016/0014-5793(95)01119-Y  1
1995 Aggarwal R, Caffrey P, Leadlay PF, Smith CJ, Staunton J. The thioesterase of the erythromycin-producing polyketide synthase: Mechanistic studies in vitro to investigate its mode of action and substrate specificity Journal of the Chemical Society, Chemical Communications. 1519-1520. DOI: 10.1039/C39950001519  1
1995 Brown MJB, Cortes J, Cutter AL, Leadlay PF, Staunton J. A mutant generated by expression of an engineered DEBS 1 protein from the erythromycin-producing polyketide synthase (PKS) in Streptomyces coelicolor produces the triketide as a lactone, but the major product is the nor-analogue derived from acetate as starter acid Journal of the Chemical Society, Chemical Communications. 1517-1518. DOI: 10.1039/C39950001517  1
1994 Marsden AFA, Caffrey P, Aparicio JF, Loughran MS, Staunton J, Leadlay PF. Stereospecific acyl transfers on the erythromycin-producing polyketide synthase Science. 263: 378-380. PMID 8278811  1
1994 Linton KJ, Cooper HN, Hunter IS, Leadlay PF. An ABC-transporter from streptomyces longisporoflavus confers resistance to the polyether-ionophore antibiotic tetronasin Molecular Microbiology. 11: 777-785. PMID 8196549 DOI: 10.1111/j.1365-2958.1994.tb00355.x  1
1994 Aparicio JF, Caffrey P, Marsden AFA, Staunton J, Leadlay PF. Limited proteolysis and active-site studies of the first multienzyme component of the erythromycin-producing polyketide synthase Journal of Biological Chemistry. 269: 8524-8528. PMID 8132579  1
1994 Dutton CJ, Hooper AM, Leadlay PF, Staunton J. Avermectin biosynthesis. Intact incorporation of a diketide chain-assembly intermediate into the polyketide macrocyclic ring Tetrahedron Letters. 35: 327-330. DOI: 10.1016/S0040-4039(00)76544-0  1
1994 Hailes HC, Handa S, Leadlay PF, Lennon IC, Ley SV, Staunton J. Biosynthesis of tetronasin: Part 3 preparation of deuterium labelled tri- and tetraketides as putative biosynthetic precursors of tetronasin Tetrahedron Letters. 35: 315-318. DOI: 10.1016/S0040-4039(00)76541-5  1
1994 Hailes HC, Handa S, Leadlay PF, Lennon IC, Ley SV, Staunton J. Biosynthesis of tetronasin: Part 2 identification of the tetraketide intermediate attached to the polyketide synthase Tetrahedron Letters. 35: 311-314. DOI: 10.1016/S0040-4039(00)76540-3  1
1994 Hailes HC, Jackson CM, Leadlay PF, Ley SV, Staunton J. Biosynthesis of tetronasin: Part 1 introduction and investigation of the diketide and triketide intermediates bound to the polyketide synthase Tetrahedron Letters. 35: 307-310. DOI: 10.1016/S0040-4039(00)76539-7  1
1993 Roberts GA, Staunton J, Leadlay PF. Heterologous expression in Escherichia coli of an intact multienzyme component of the erythromycin-producing polyketide synthase. European Journal of Biochemistry / Febs. 214: 305-11. PMID 8508800  1
1993 Roberts GA, Staunton J, Leadlay PF. 6-Deoxyerythronolide B synthase 3 from Saccharopolyspora erythraea: over-expression in Escherichia coli, purification and characterisation. Biochemical Society Transactions. 21: 32S. PMID 8449313  1
1993 Cooper HN, Cortes J, Bevitt DJ, Leadlay PF, Staunton J. Analysis of a gene cluster from S. longisporoflavus potentially involved in tetronasin biosynthesis Biochemical Society Transactions. 21: 31S. PMID 8449312  1
1993 Bevitt DJ, Staunton J, Leadlay PF. Mutagenesis of the dehydratase active site in the erythromycin-producing polyketide synthase Biochemical Society Transactions. 21: 30S. PMID 8449310  1
1993 Leadlay PF, Staunton J, Aparicio JF, Bevitt DJ, Caffrey P, Cortes J, Marsden A, Roberts GA. The erythromycin-producing polyketide synthase. Biochemical Society Transactions. 21: 218-22. PMID 8449298  1
1993 Morris SA, Revill WP, Staunton J, Leadlay PF. Purification and separation of holo- and apo-forms of Saccharopolyspora erythraea acyl-carrier protein released from recombinant Escherichia coli by freezing and thawing. The Biochemical Journal. 294: 521-7. PMID 8373367  1
1993 Keep NH, Smith GA, Evans MC, Diakun GP, Leadlay PF. The synthetic substrate succinyl(carbadethia)-CoA generates cob(II)alamin on adenosylcobalamin-dependent methylmalonyl-CoA mutase. The Biochemical Journal. 295: 387-92. PMID 7902085  1
1992 Bevitt DJ, Cortes J, Haydock SF, Leadlay PF. 6-Deoxyerythronolide-B synthase 2 from Saccharopolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the multifunctional enzyme European Journal of Biochemistry. 204: 39-49. PMID 1740151  1
1992 Caffrey P, Bevitt DJ, Staunton J, Leadlay PF. Identification of DEBS 1, DEBS 2 and DEBS 3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase from Saccharopolyspora erythraea Febs Letters. 304: 225-228. PMID 1618327 DOI: 10.1016/0014-5793(92)80624-P  1
1992 Bylsma N, Drakenberg T, Andersson I, Leadlay PF, Forsén S. Prokaryotic calcium-binding protein of the calmodulin superfamily Calcium binding to a Saccharopolyspora erythraea 20 kDa protein Febs Letters. 299: 44-47. PMID 1544472 DOI: 10.1016/0014-5793(92)80096-Y  1
1992 Hellyer A, Leadlay PF, Slabas AR. Induction, purification and characterisation of acyl-ACP thioesterase from developing seeds of oil seed rape (Brassica napus) Plant Molecular Biology. 20: 763-780. PMID 1301073 DOI: 10.1007/BF00027148  1
1991 Revill WP, Leadlay PF. Cloning, characterization, and high-level expression in Escherichia coli of the Saccharopolyspora erythraea gene encoding an acyl carrier protein potentially involved in fatty acid biosynthesis Journal of Bacteriology. 173: 4379-4385. PMID 2066335  1
1991 Caffrey P, Green B, Packman LC, Rawlings BJ, Staunton J, Leadlay PF. An acyl-carrier-protein - thioesterase domain from the 6-deoxyerythronolide B synthase of Saccharopolyspora erythraea. High-level production, purification and characterisation in Escherichia coli European Journal of Biochemistry. 195: 823-830. PMID 1999200  1
1991 Haydock SF, Dowson JA, Dhillon N, Roberts GA, Cortes J, Leadlay PF. Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Molecular & General Genetics : Mgg. 230: 120-8. PMID 1840640 DOI: 10.1007/BF00290659  1
1991 Bridges AM, Leadlay PF, Revill WP, Staunton J. Use of electrospray mass spectrometry in enzymic studies on acyl carrier protein implicated in fatty acid biosynthesis in Saccharopolyspora erythraea Journal of the Chemical Society, Chemical Communications. 778-779. DOI: 10.1039/C39910000778  1
1991 Bridges AM, Leadlay PF, Revill WP, Staunton J. Use of electrospray mass spectrometry to assay chemical reactivity of an acyl carrier protein involved in fatty acid biosynthesis in Saccharopolyspora erythraea Journal of the Chemical Society, Chemical Communications. 776-777. DOI: 10.1039/C39910000776  1
1990 Dhillon N, Leadlay PF. A repeated decapeptide motif in the C-terminal domain of the ribosomal RNA methyltransferase from the erythromycin producer Saccharopolyspora erythraea Febs Letters. 262: 189-193. PMID 2335200 DOI: 10.1016/0014-5793(90)80186-M  1
1990 Cortes J, Haydock SF, Roberts GA, Bevitt DJ, Leadlay PF. An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea. Nature. 348: 176-8. PMID 2234082 DOI: 10.1038/348176a0  1
1990 Williams KP, Leadlay PF, Lowe PN. Inhibition of pyruvate:ferredoxin oxidoreductase from Trichomonas vaginalis by pyruvate and its analogues. Comparison with the pyruvate decarboxylase component of the pyruvate dehydrogenase complex Biochemical Journal. 268: 69-75. PMID 2188649  1
1990 McKie N, Keep NH, Patchett ML, Leadlay PF. Adenosylcobalamin-dependent methylmalonyl-CoA mutase from Propionibacterium shermanii. Active holoenzyme produced from Escherichia coli Biochemical Journal. 269: 293-298. PMID 1974759  1
1989 Swan DG, Cortes J, Hale RS, Leadlay PF. Cloning, characterization, and heterologous expression of the Saccharopolyspora erythraea (Streptomyces erythraeus) gene encoding an EF-hand calcium-binding protein Journal of Bacteriology. 171: 5614-5619. PMID 2676983  1
1989 Dhillon N, Hale RS, Cortes J, Leadlay PF. Molecular characterization of a gene from Saccharopolyspora erythraea (Streptomyces erythraeus) which is involved in erythromycin biosynthesis Molecular Microbiology. 3: 1405-1414. PMID 2575703  1
1989 Marsh EN, Harding SE, Leadlay PF. Subunit interactions in Propionibacterium shermanii methylmalonyl-CoA mutase studied by analytical ultracentrifugation Biochemical Journal. 260: 353-358. PMID 2569862  1
1989 Marsh EN, McKie N, Davis NK, Leadlay PF. Cloning and structural characterization of the genes coding for adenosylcobalamin-dependent methylmalonyl-CoA mutase from Propionibacterium shermanii. Biochemical Journal. 260: 345-352. PMID 2569861  1
1989 Marsh EN, Leadlay PF. Methylmalonyl-CoA mutase from Propionibacterium shermanii. Evidence for the presence of two masked cysteine residues Biochemical Journal. 260: 339-343. PMID 2569860  1
1988 Marsh N, Leadlay PF, Evans PR. Crystallization and preliminary diffraction data for adenosylcobalamin-dependent methylmalonyl-CoA mutase from Propionibacterium shermanii Journal of Molecular Biology. 200: 421-422. PMID 2897473 DOI: 10.1016/0022-2836(88)90252-5  1
1987 Williams K, Lowe PN, Leadlay PF. Purification and characterization of pyruvate: ferredoxin oxidoreductase from the anaerobic protozoon Trichomonas vaginalis Biochemical Journal. 246: 529-536. PMID 3500709  1
1987 Hale RS, Jordan KN, Leadlay PF. A small, discrete acyl carrier protein is involved in de novo fatty acid biosynthesis in Streptomyces erythraeus Febs Letters. 224: 133-136. PMID 3315744 DOI: 10.1016/0014-5793(87)80436-2  1
1977 Leadlay PF, Breathnach R, Gatehouse JA, Johnson PE, Knowles JR. Phosphoglycerate mutase from wheat germ: studies with isotopically labeled 3-phospho-D-glycerates showing that the catalyzed reaction is intramolecular. Appendix: phosphoglycerate mutase from wheat germ: isolation, crystallization, and properties. Biochemistry. 16: 3045-53. PMID 19039  0.92
1976 Leadlay PF, Albery WJ, Knowles JR. Energetics of triosephosphate isomerase: deuterium isotope effects in the enzyme-catalyzed reaction. Biochemistry. 15: 5617-20. PMID 999836  0.92
1972 Knowles JR, Leadlay PF, Maister SG. Triosephosphate isomerase: isotope studies on the mechanistic pathway. Cold Spring Harbor Symposia On Quantitative Biology. 36: 157-64. PMID 4508134  0.92
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