Florian Hollfelder - Publications

University of Cambridge, Cambridge, England, United Kingdom 
enzyme mechanism, directed evololution, physical-organic chemistry, microfluidics

45 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2016 Campbell E, Kaltenbach M, Correy GJ, Carr PD, Porebski BT, Livingstone EK, Afriat-Jurnou L, Buckle AM, Weik M, Hollfelder F, Tokuriki N, Jackson CJ. The role of protein dynamics in the evolution of new enzyme function. Nature Chemical Biology. PMID 27618189 DOI: 10.1038/nchembio.2175  0.96
2015 Colin PY, Kintses B, Gielen F, Miton CM, Fischer G, Mohamed MF, Hyvönen M, Morgavi DP, Janssen DB, Hollfelder F. Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics. Nature Communications. 6: 10008. PMID 26639611 DOI: 10.1038/ncomms10008  0.96
2015 Urbach C, Gordon NC, Strickland I, Lowne D, Joberty-Candotti C, May R, Herath A, Hijnen D, Thijs JL, Bruijnzeel-Koomen CA, Minter RR, Hollfelder F, Jermutus L. Combinatorial Screening Identifies Novel Promiscuous Matrix Metalloproteinase Activities that Lead to Inhibition of the Therapeutic Target IL-13. Chemistry & Biology. PMID 26548614 DOI: 10.1016/j.chembiol.2015.09.013  0.96
2015 Fradet E, Bayer C, Hollfelder F, Baroud CN. Measuring Fast and Slow Enzyme Kinetics in Stationary Droplets. Analytical Chemistry. 87: 11915-22. PMID 26524082 DOI: 10.1021/acs.analchem.5b03567  0.96
2015 Kaltenbach M, Jackson CJ, Campbell EC, Hollfelder F, Tokuriki N. Reverse evolution leads to genotypic incompatibility despite functional and active-site convergence. Elife. 4. PMID 26274563 DOI: 10.7554/eLife.06492  0.96
2015 Houlihan G, Gatti-Lafranconi P, Lowe D, Hollfelder F. Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process. Protein Engineering, Design & Selection : Peds. 28: 269-79. PMID 26134501 DOI: 10.1093/protein/gzv029  0.96
2015 Shafee T, Gatti-Lafranconi P, Minter R, Hollfelder F. Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease. Chembiochem : a European Journal of Chemical Biology. PMID 26097079 DOI: 10.1002/cbic.201500295  0.96
2015 Chen W, Avezov E, Schlachter SC, Gielen F, Laine RF, Harding HP, Hollfelder F, Ron D, Kaminski CF. A method to quantify FRET stoichiometry with phasor plot analysis and acceptor lifetime ingrowth. Biophysical Journal. 108: 999-1002. PMID 25762312 DOI: 10.1016/j.bpj.2015.01.012  0.96
2014 Fischlechner M, Schaerli Y, Mohamed MF, Patil S, Abell C, Hollfelder F. Evolution of enzyme catalysts caged in biomimetic gel-shell beads. Nature Chemistry. 6: 791-6. PMID 25143214 DOI: 10.1038/nchem.1996  0.96
2013 Hollfelder F, Lutz S. Just (protein) engineering? Current Opinion in Structural Biology. 23: 569-70. PMID 23886559 DOI: 10.1016/j.sbi.2013.07.003  0.96
2013 Goldsworthy M, Absalom NL, Schröter D, Matthews HC, Bogani D, Moir L, Long A, Church C, Hugill A, Anstee QM, Goldin R, Thursz M, Hollfelder F, Cox RD. Mutations in Mll2, an H3K4 methyltransferase, result in insulin resistance and impaired glucose tolerance in mice. Plos One. 8: e61870. PMID 23826075 DOI: 10.1371/journal.pone.0061870  0.96
2013 Shim JU, Ranasinghe RT, Smith CA, Ibrahim SM, Hollfelder F, Huck WT, Klenerman D, Abell C. Ultrarapid generation of femtoliter microfluidic droplets for single-molecule-counting immunoassays. Acs Nano. 7: 5955-64. PMID 23805985 DOI: 10.1021/nn401661d  0.96
2012 Kintses B, Hein C, Mohamed MF, Fischlechner M, Courtois F, Lainé C, Hollfelder F. Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution. Chemistry & Biology. 19: 1001-9. PMID 22921067 DOI: 10.1016/j.chembiol.2012.06.009  0.96
2012 Babtie AC, Lima MF, Kirby AJ, Hollfelder F. Kinetic and computational evidence for an intermediate in the hydrolysis of sulfonate esters. Organic & Biomolecular Chemistry. 10: 8095-101. PMID 22832951 DOI: 10.1039/c2ob25699a  0.96
2012 Stein V, Kaltenbach M, Hollfelder F. Assembling linear DNA templates for in vitro transcription and translation. Methods in Molecular Biology (Clifton, N.J.). 815: 67-78. PMID 22130984 DOI: 10.1007/978-1-61779-424-7_6  0.96
2012 Delley RJ, Bandyopadhyay S, Fox MA, Schliehe C, Hodgson DR, Hollfelder F, Kirby AJ, O'Donoghue AC. peri-Dimethylamino substituent effects on proton transfer at carbon in α-naphthylacetate esters: a model for mandelate racemase. Organic & Biomolecular Chemistry. 10: 590-6. PMID 22108698 DOI: 10.1039/c1ob06525d  0.96
2011 Kaltenbach M, Stein V, Hollfelder F. SNAP dendrimers: multivalent protein display on dendrimer-like DNA for directed evolution. Chembiochem : a European Journal of Chemical Biology. 12: 2208-16. PMID 21780273 DOI: 10.1002/cbic.201100240  0.96
2011 Shim JU, Patil SN, Hodgkinson JT, Bowden SD, Spring DR, Welch M, Huck WT, Hollfelder F, Abell C. Controlling the contents of microdroplets by exploiting the permeability of PDMS. Lab On a Chip. 11: 1132-7. PMID 21298160 DOI: 10.1039/c0lc00615g  0.96
2011 Huebner AM, Abell C, Huck WT, Baroud CN, Hollfelder F. Monitoring a reaction at submillisecond resolution in picoliter volumes. Analytical Chemistry. 83: 1462-8. PMID 21244017 DOI: 10.1021/ac103234a  0.96
2010 Schaerli Y, Stein V, Spiering MM, Benkovic SJ, Abell C, Hollfelder F. Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification. Nucleic Acids Research. 38: e201. PMID 20921065 DOI: 10.1093/nar/gkq795  0.96
2010 O'Brien PJ, Hollfelder F. Hitting a moving target?--Understanding how conformational diversity impacts enzymatic catalysis. Current Opinion in Chemical Biology. 14: 634-5. PMID 20870450 DOI: 10.1016/j.cbpa.2010.09.009  1
2010 Theberge AB, Courtois F, Schaerli Y, Fischlechner M, Abell C, Hollfelder F, Huck WT. Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angewandte Chemie (International Ed. in English). 49: 5846-68. PMID 20572214 DOI: 10.1002/anie.200906653  0.96
2010 Bai Y, He X, Liu D, Patil SN, Bratton D, Huebner A, Hollfelder F, Abell C, Huck WT. A double droplet trap system for studying mass transport across a droplet-droplet interface. Lab On a Chip. 10: 1281-5. PMID 20445881 DOI: 10.1039/b925133b  0.96
2010 van Loo B, Jonas S, Babtie AC, Benjdia A, Berteau O, Hyvönen M, Hollfelder F. An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily. Proceedings of the National Academy of Sciences of the United States of America. 107: 2740-5. PMID 20133613 DOI: 10.1073/pnas.0903951107  0.6
2009 Shim JU, Olguin LF, Whyte G, Scott D, Babtie A, Abell C, Huck WT, Hollfelder F. Simultaneous determination of gene expression and enzymatic activity in individual bacterial cells in microdroplet compartments. Journal of the American Chemical Society. 131: 15251-6. PMID 19799429 DOI: 10.1021/ja904823z  0.96
2009 Avenier F, Hollfelder F. Combining medium effects and cofactor catalysis: metal-coordinated synzymes accelerate phosphate transfer by 10(8). Chemistry (Weinheim An Der Bergstrasse, Germany). 15: 12371-80. PMID 19798717 DOI: 10.1002/chem.200802616  0.6
2009 Stein V, Hollfelder F. An efficient method to assemble linear DNA templates for in vitro screening and selection systems. Nucleic Acids Research. 37: e122. PMID 19617373 DOI: 10.1093/nar/gkp589  0.96
2009 Damean N, Olguin LF, Hollfelder F, Abell C, Huck WT. Simultaneous measurement of reactions in microdroplets filled by concentration gradients. Lab On a Chip. 9: 1707-13. PMID 19495454 DOI: 10.1039/b821021g  0.96
2009 Hufnagel H, Huebner A, Gülch C, Güse K, Abell C, Hollfelder F. An integrated cell culture lab on a chip: modular microdevices for cultivation of mammalian cells and delivery into microfluidic microdroplets. Lab On a Chip. 9: 1576-82. PMID 19458865 DOI: 10.1039/b821695a  0.96
2009 Babtie AC, Bandyopadhyay S, Olguin LF, Hollfelder F. Efficient catalytic promiscuity for chemically distinct reactions. Angewandte Chemie (International Ed. in English). 48: 3692-4. PMID 19373810 DOI: 10.1002/anie.200805843  0.96
2009 Courtois F, Olguin LF, Whyte G, Theberge AB, Huck WT, Hollfelder F, Abell C. Controlling the retention of small molecules in emulsion microdroplets for use in cell-based assays. Analytical Chemistry. 81: 3008-16. PMID 19284775 DOI: 10.1021/ac802658n  0.96
2009 Huebner A, Bratton D, Whyte G, Yang M, Demello AJ, Abell C, Hollfelder F. Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. Lab On a Chip. 9: 692-8. PMID 19224019 DOI: 10.1039/b813709a  0.96
2009 Golicnik M, Olguin LF, Feng G, Baxter NJ, Waltho JP, Williams NH, Hollfelder F. Kinetic analysis of beta-phosphoglucomutase and its inhibition by magnesium fluoride. Journal of the American Chemical Society. 131: 1575-88. PMID 19132841 DOI: 10.1021/ja806421f  0.96
2009 Schaerli Y, Wootton RC, Robinson T, Stein V, Dunsby C, Neil MA, French PM, Demello AJ, Abell C, Hollfelder F. Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. Analytical Chemistry. 81: 302-6. PMID 19055421 DOI: 10.1021/ac802038c  0.96
2008 Olguin LF, Askew SE, O'Donoghue AC, Hollfelder F. Efficient catalytic promiscuity in an enzyme superfamily: an arylsulfatase shows a rate acceleration of 10(13) for phosphate monoester hydrolysis. Journal of the American Chemical Society. 130: 16547-55. PMID 19554727 DOI: 10.1021/ja8047943  0.6
2008 Kirby AJ, Hollfelder F. Biochemistry: Enzymes under the nanoscope. Nature. 456: 45-7. PMID 18987728 DOI: 10.1038/456045a  0.96
2008 Van Vliet LD, Chapman MR, Avenier F, Kitson CZ, Hollfelder F. Relating chemical and biological diversity space: a tunable system for efficient gene transfection. Chembiochem : a European Journal of Chemical Biology. 9: 1960-7. PMID 18655081 DOI: 10.1002/cbic.200800003  0.6
2008 Huebner A, Olguin LF, Bratton D, Whyte G, Huck WT, de Mello AJ, Edel JB, Abell C, Hollfelder F. Development of quantitative cell-based enzyme assays in microdroplets. Analytical Chemistry. 80: 3890-6. PMID 18399662 DOI: 10.1021/ac800338z  0.96
2008 Baxter NJ, Blackburn GM, Marston JP, Hounslow AM, Cliff MJ, Bermel W, Williams NH, Hollfelder F, Wemmer DE, Waltho JP. Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes. Journal of the American Chemical Society. 130: 3952-8. PMID 18318536 DOI: 10.1021/ja078000n  0.96
2008 Courtois F, Olguin LF, Whyte G, Bratton D, Huck WT, Abell C, Hollfelder F. An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets. Chembiochem : a European Journal of Chemical Biology. 9: 439-46. PMID 18232037 DOI: 10.1002/cbic.200700536  0.96
2007 Avenier F, Domingos JB, Vliet LD, Hollfelder F. Polyethylene imine derivatives ('synzymes') accelerate phosphate transfer in the absence of metal. Journal of the American Chemical Society. 129: 7611-9. PMID 17530755 DOI: 10.1021/ja069095g  0.6
2007 Van Vliet LD, Ellis T, Foley PJ, Liu L, Pfeffer FM, Russell RA, Warrener RN, Hollfelder F, Waring MJ. Molecular recognition of DNA by rigid [N]-polynorbornane-derived bifunctional intercalators: synthesis and evaluation of their binding properties. Journal of Medicinal Chemistry. 50: 2326-40. PMID 17429957 DOI: 10.1021/jm0613020  0.96
2007 Otten LG, Schaffer ML, Villiers BR, Stachelhaus T, Hollfelder F. An optimized ATP/PP(i)-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis. Biotechnology Journal. 2: 232-40. PMID 17294409 DOI: 10.1002/biot.200600220  0.96
2007 Nightingale KP, Gendreizig S, White DA, Bradbury C, Hollfelder F, Turner BM. Cross-talk between histone modifications in response to histone deacetylase inhibitors: MLL4 links histone H3 acetylation and histone H3K4 methylation. The Journal of Biological Chemistry. 282: 4408-16. PMID 17166833 DOI: 10.1074/jbc.M606773200  0.96
2006 Baxter NJ, Olguin LF, Golicnik M, Feng G, Hounslow AM, Bermel W, Blackburn GM, Hollfelder F, Waltho JP, Williams NH. A Trojan horse transition state analogue generated by MgF3- formation in an enzyme active site. Proceedings of the National Academy of Sciences of the United States of America. 103: 14732-7. PMID 16990434 DOI: 10.1073/pnas.0604448103  0.96
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