Paul C. Engel, D.Phil - Publications

Affiliations: 
1970-1994 University of Sheffield, Sheffield, England, United Kingdom 
 1994- Biomolecular & Biomedical Science University College Dublin, Dublin, County Dublin, Ireland 
Area:
Enzymology
Website:
http://www.ucd.ie/research/people/biomolecularbiomedscience/professorpaulengel/

191 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any innacuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2014 Yu X, Wang X, Engel PC. The specificity and kinetic mechanism of branched-chain amino acid aminotransferase from Escherichia coli studied with a new improved coupled assay procedure and the enzyme's potential for biocatalysis. The Febs Journal. 281: 391-400. PMID 24206068 DOI: 10.1111/febs.12609  1
2014 Engel PC. Glutamate dehydrogenases: the why and how of coenzyme specificity. Neurochemical Research. 39: 426-32. PMID 23761034 DOI: 10.1007/s11064-013-1089-x  1
2013 Sharkey MA, Oliveira TF, Engel PC, Khan AR. Structure of NADP(+)-dependent glutamate dehydrogenase from Escherichia coli--reflections on the basis of coenzyme specificity in the family of glutamate dehydrogenases. The Febs Journal. 280: 4681-92. PMID 23879525 DOI: 10.1111/febs.12439  1
2013 Munawar N, Engel PC. Prospects for robust biocatalysis: engineering of novel specificity in a halophilic amino acid dehydrogenase. Extremophiles : Life Under Extreme Conditions. 17: 43-51. PMID 23104166 DOI: 10.1007/s00792-012-0491-7  1
2012 Yu X, Bresser J, Schall I, Djurdjevic I, Buckel W, Wang X, Engel PC. Development of a satisfactory and general continuous assay for aminotransferases by coupling with (R)-2-hydroxyglutarate dehydrogenase. Analytical Biochemistry. 431: 127-31. PMID 23000002 DOI: 10.1016/j.ab.2012.09.009  1
2012 Sharkey MA, Gori A, Capone M, Engel PC. Reversal of the extreme coenzyme selectivity of Clostridium symbiosum glutamate dehydrogenase. The Febs Journal. 279: 3003-9. PMID 22747945 DOI: 10.1111/j.1742-4658.2012.08681.x  1
2012 Munawar N, Engel PC. Overexpression in a non-native halophilic host and biotechnological potential of NAD+-dependent glutamate dehydrogenase from Halobacterium salinarum strain NRC-36014. Extremophiles : Life Under Extreme Conditions. 16: 463-76. PMID 22527040 DOI: 10.1007/s00792-012-0446-z  1
2012 Oliveira T, Panjikar S, Carrigan JB, Hamza M, Sharkey MA, Engel PC, Khan AR. Crystal structure of NAD+-dependent Peptoniphilus asaccharolyticus glutamate dehydrogenase reveals determinants of cofactor specificity. Journal of Structural Biology. 177: 543-52. PMID 22068154 DOI: 10.1016/j.jsb.2011.10.006  1
2011 Griffin J, Engel PC. An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD-Dependent Glutamate Dehydrogenase. Enzyme Research. 2011: 595793. PMID 21876794 DOI: 10.4061/2011/595793  1
2011 Wang XT, Mion B, Aherne A, Engel PC. Molecular recruitment as a basis for negative dominant inheritance? propagation of misfolding in oligomers of IMPDH1, the mutated enzyme in the RP10 form of retinitis pigmentosa. Biochimica Et Biophysica Acta. 1812: 1472-6. PMID 21791244 DOI: 10.1016/j.bbadis.2011.07.006  1
2011 Storm J, Perner J, Aparicio I, Patzewitz EM, Olszewski K, Llinas M, Engel PC, Müller S. Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development. Malaria Journal. 10: 193. PMID 21756354 DOI: 10.1186/1475-2875-10-193  1
2011 Sharkey MA, Maher MA, Guyonvarch A, Engel PC. Kinetic characterisation of recombinant Corynebacterium glutamicum NAD+-dependent LDH over-expressed in E. coli and its rescue of an lldD- phenotype in C. glutamicum: the issue of reversibility re-examined. Archives of Microbiology. 193: 731-40. PMID 21567176 DOI: 10.1007/s00203-011-0711-z  1
2011 Capone M, Scanlon D, Griffin J, Engel PC. Re-engineering the discrimination between the oxidized coenzymes NAD+ and NADP+ in clostridial glutamate dehydrogenase and a thorough reappraisal of the coenzyme specificity of the wild-type enzyme. The Febs Journal. 278: 2460-8. PMID 21564547 DOI: 10.1111/j.1742-4658.2011.08172.x  1
2011 Engel PC. Making biochemistry count: life among the amino acid dehydrogenases. Biochemical Society Transactions. 39: 425-9. PMID 21428913 DOI: 10.1042/BST0390425  1
2011 Engel PC. A marriage full of surprises: forty-five years living with glutamate dehydrogenase. Neurochemistry International. 59: 489-94. PMID 21419817 DOI: 10.1016/j.neuint.2011.03.014  1
2010 Griffin J, Engel PC. The -SH Protection Method for Determining Accurate K(d) Values for Enzyme-Coenzyme Complexes of NAD-Dependent Glutamate Dehydrogenase and Engineered Mutants: Evidence for Nonproductive NADPH Complexes. Enzyme Research. 2010: 951472. PMID 21048875 DOI: 10.4061/2010/951472  1
2010 Oliveira TF, Carrigan JB, Hamza MA, Sharkey MA, Engel PC, Khan AR. Crystallization and preliminary structural analyses of glutamate dehydrogenase from Peptoniphilus asaccharolyticus. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. 66: 523-6. PMID 20445250 DOI: 10.1107/S1744309110010006  1
2010 Aparicio IM, Marín-Menéndez A, Bell A, Engel PC. Susceptibility of Plasmodium falciparum to glutamate dehydrogenase inhibitors--a possible new antimalarial target. Molecular and Biochemical Parasitology. 172: 152-5. PMID 20399810 DOI: 10.1016/j.molbiopara.2010.04.002  1
2010 Wang XG, Zhang P, Lynch S, Engel PC. Forced evolution of Escherichia coli cells with the ability to effectively utilize non-natural amino acids l-tert-leucine, l-norleucine and γ-methyl-l-leucine Biocatalysis and Biotransformation. 28: 293-303. DOI: 10.3109/10242422.2010.514047  1
2010 Engel PC, Paradisi F. Novel enzymes for biotransformation and resolution of alpha-amino acids Comprehensive Natural Products Ii: Chemistry and Biology. 5: 71-90.  1
2009 Sala Frigerio C, Kukar TL, Fauq A, Engel PC, Golde TE, Walsh DM. An NSAID-like compound, FT-9, preferentially inhibits gamma-secretase cleavage of the amyloid precursor protein compared to its effect on amyloid precursor-like protein 1. Biochemistry. 48: 10894-904. PMID 19821615 DOI: 10.1021/bi901237k  1
2009 Chen S, Engel PC. Efficient screening for new amino acid dehydrogenase activity: directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid. Journal of Biotechnology. 142: 127-34. PMID 19501264 DOI: 10.1016/j.jbiotec.2009.03.005  1
2009 Wang XT, Engel PC. Clinical mutants of human glucose 6-phosphate dehydrogenase: impairment of NADP(+) binding affects both folding and stability. Biochimica Et Biophysica Acta. 1792: 804-9. PMID 19465117 DOI: 10.1016/j.bbadis.2009.05.003  1
2009 Sharkey MA, Engel PC. Modular coenzyme specificity: a domain-swopped chimera of glutamate dehydrogenase. Proteins. 77: 268-78. PMID 19425107 DOI: 10.1002/prot.22433  1
2009 Wang XT, Engel PC. An optimised system for refolding of human glucose 6-phosphate dehydrogenase. Bmc Biotechnology. 9: 19. PMID 19284595 DOI: 10.1186/1472-6750-9-19  1
2009 Lamm AS, Khare A, Rosazza JPN, Taglieber A, Schulz F, Hollmann F, Rusek M, Reetz MT, Gefflaut T, Sagot E, Bolte J, Mihara H, Esaki N, Conway P, Paradisi F, ... Engel P, et al. Reduction of Functional Groups Practical Methods For Biocatalysis and Biotransformations. 295-298. DOI: 10.1002/9780470748589.ch10  1
2008 Paradisi F, Conway PA, Maguire AR, Engel PC. Engineered dehydrogenase biocatalysts for non-natural amino acids: efficient isolation of the D-enantiomer from racemic mixtures. Organic & Biomolecular Chemistry. 6: 3611-5. PMID 19082164 DOI: 10.1039/b809147a  1
2008 Wang XT, Chan TF, Lam VM, Engel PC. What is the role of the second "structural" NADP+-binding site in human glucose 6-phosphate dehydrogenase? Protein Science : a Publication of the Protein Society. 17: 1403-11. PMID 18493020 DOI: 10.1110/ps.035352.108  1
2008 Hamza MA, Engel PC. Homotropic allosteric control in clostridial glutamate dehydrogenase: different mechanisms for glutamate and NAD+? Febs Letters. 582: 1816-20. PMID 18472008 DOI: 10.1016/j.febslet.2008.04.049  1
2008 Carrigan JB, Engel PC. The structural basis of proteolytic activation of bovine glutamate dehydrogenase. Protein Science : a Publication of the Protein Society. 17: 1346-53. PMID 18467497 DOI: 10.1110/ps.034785.108  1
2008 Sharkey MA, Engel PC. Apparent negative co-operativity and substrate inhibition in overexpressed glutamate dehydrogenase from Escherichia coli. Fems Microbiology Letters. 281: 132-9. PMID 18294195 DOI: 10.1111/j.1574-6968.2008.01086.x  1
2008 Huang Y, Choi MY, Au SW, Au DM, Lam VM, Engel PC. Purification and detailed study of two clinically different human glucose 6-phosphate dehydrogenase variants, G6PD(Plymouth) and G6PD(Mahidol): Evidence for defective protein folding as the basis of disease. Molecular Genetics and Metabolism. 93: 44-53. PMID 17959407 DOI: 10.1016/j.ymgme.2007.08.122  1
2007 Carrigan JB, Engel PC. Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis. The Febs Journal. 274: 5167-74. PMID 17850332 DOI: 10.1111/j.1742-4658.2007.06038.x  1
2007 Hamza MA, Martin SR, Engel PC. The contribution of tryptophan residues to conformational changes in clostridial glutamate dehydrogenase--W64 and W449 as mediators of the cooperative response to glutamate. The Febs Journal. 274: 4126-34. PMID 17645547 DOI: 10.1111/j.1742-4658.2007.05940.x  1
2007 Paradisi F, Collins S, Maguire AR, Engel PC. Phenylalanine dehydrogenase mutants: efficient biocatalysts for synthesis of non-natural phenylalanine derivatives. Journal of Biotechnology. 128: 408-11. PMID 16996633 DOI: 10.1016/j.jbiotec.2006.08.008  1
2007 Hamza MA, Engel PC. Enhancing long-term thermal stability in mesophilic glutamate dehydrogenase from Clostridium symbiosum by eliminating cysteine residues Enzyme and Microbial Technology. 41: 706-710. DOI: 10.1016/j.enzmictec.2007.06.008  1
2007 Chen S, Engel PC. An engineered mutant, L307V of phenylalanine dehydrogenase from Bacillus sphaericus: high activity and stability in organic-aqueous solvent mixtures and utility for synthesis of non-natural l-amino acids Enzyme and Microbial Technology. 40: 1407-1411. DOI: 10.1016/j.enzmictec.2006.10.021  1
2006 Wang XT, Lam VM, Engel PC. Functional properties of two mutants of human glucose 6-phosphate dehydrogenase, R393G and R393H, corresponding to the clinical variants G6PD Wisconsin and Nashville. Biochimica Et Biophysica Acta. 1762: 767-74. PMID 16934959 DOI: 10.1016/j.bbadis.2006.06.014  1
2006 Faulkner E, Barrett M, Okor S, Kieran P, Casey E, Paradisi F, Engel P, Glennon B. Use of fed-batch cultivation for achieving high cell densities for the pilot-scale production of a recombinant protein (phenylalanine dehydrogenase) in Escherichia coli Biotechnology Progress. 22: 889-897. PMID 16739976 DOI: 10.1021/bp050327+  1
2005 Cainelli G, Engel PC, Galletti P, Giacomini D, Gualandi A, Paradisi F. Engineered phenylalanine dehydrogenase in organic solvents: homogeneous and biphasic enzymatic reactions. Organic & Biomolecular Chemistry. 3: 4316-20. PMID 16327891 DOI: 10.1039/b510816k  1
2005 O'Reilly LP, Andresen BS, Engel PC. Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD). K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein. The Febs Journal. 272: 4549-57. PMID 16128823 DOI: 10.1111/j.1742-4658.2005.04878.x  1
2005 Wang XT, Lam VM, Engel PC. Marked decrease in specific activity contributes to disease phenotype in two human glucose 6-phosphate dehydrogenase mutants, G6PD(Union) and G6PD(Andalus). Human Mutation. 26: 284. PMID 16088936 DOI: 10.1002/humu.9367  1
2005 Paradisi F, Woolfson R, Geoghegan KF, Engel PC. Identification of the residue responsible for catalysing regeneration of activity in the inactive glutamate dehydrogenase mutant D165N. Febs Letters. 579: 2830-2. PMID 15878554 DOI: 10.1016/j.febslet.2005.03.098  1
2005 Ingoldsby LM, Geoghegan KF, Hayden BM, Engel PC. The discovery of four distinct glutamate dehydrogenase genes in a strain of Halobacterium salinarum. Gene. 349: 237-44. PMID 15780999 DOI: 10.1016/j.gene.2005.01.011  1
2005 Paradisi F, Dean JL, Geoghegan KF, Engel PC. Spontaneous chemical reversion of an active site mutation: deamidation of an asparagine residue replacing the catalytic aspartic acid of glutamate dehydrogenase. Biochemistry. 44: 3636-43. PMID 15736973 DOI: 10.1021/bi047679u  1
2005 Carrigan JB, Coughlan S, Engel PC. Properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe Peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an Escherichia coli host. Fems Microbiology Letters. 244: 53-9. PMID 15727821 DOI: 10.1016/j.femsle.2005.01.026  1
2005 Paradisi F, Gualandi A, Galletti P, Giacomini D, Cainelli G, Engel PC. Immobilised enzyme to perform enantiomerically pure reductive aminations in presence of organic solvents Aiche Annual Meeting, Conference Proceedings. 9554.  1
2005 Engel PC, Paradisi F, Moynihan E, Maguire AR. Mutants of phenylalanine dehydrogenase: New bio-catalysts in the asymmetric synthesis and racemic resolution of non-natural amino acids Aiche Annual Meeting, Conference Proceedings. 12223-12227.  1
2005 Faulkner E, Barrett M, Okor S, Paradisi F, Engel P, Casey E, Glennon B. The use of fed batch cultivation for achieving high cell densities for the pilot scale production of a recombinant protein (Phenylalanine dehydrogenase) in Escherichia coli Aiche Annual Meeting, Conference Proceedings. 9086-9095.  1
2004 O'Reilly L, Bross P, Corydon TJ, Olpin SE, Hansen J, Kenney JM, McCandless SE, Frazier DM, Winter V, Gregersen N, Engel PC, Andresen BS. The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitive. European Journal of Biochemistry / Febs. 271: 4053-63. PMID 15479234 DOI: 10.1111/j.1432-1033.2004.04343.x  1
2004 Busca P, Paradisi F, Moynihan E, Maguire AR, Engel PC. Enantioselective synthesis of non-natural amino acids using phenylalanine dehydrogenases modified by site-directed mutagenesis. Organic & Biomolecular Chemistry. 2: 2684-91. PMID 15351834 DOI: 10.1039/B406364C  1
2004 Aherne A, Kennan A, Kenna PF, McNally N, Lloyd DG, Alberts IL, Kiang AS, Humphries MM, Ayuso C, Engel PC, Gu JJ, Mitchell BS, Farrar GJ, Humphries P. On the molecular pathology of neurodegeneration in IMPDH1-based retinitis pigmentosa. Human Molecular Genetics. 13: 641-50. PMID 14981049 DOI: 10.1093/hmg/ddh061  1
2004 Batoux NE, Paradisi F, Engel PC, Migaud ME. Novel nicotinamide adenine dinucleotide analogues as selective inhibitors of NAD+-dependent enzymes Tetrahedron. 60: 6609-6617. DOI: 10.1016/j.tet.2004.05.091  1
2003 Seah SY, Britton KL, Rice DW, Asano Y, Engel PC. Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling. European Journal of Biochemistry / Febs. 270: 4628-34. PMID 14622249 DOI: 10.1046/j.1432-1033.2003.03852.x  1
2003 Irwin JA, Lynch SV, Coughlan S, Baker PJ, Gudmundsson HM, Alfredsson GA, Rice DW, Engel PC. Alanine dehydrogenase from the psychrophilic bacterium strain PA-43: overexpression, molecular characterization, and sequence analysis. Extremophiles : Life Under Extreme Conditions. 7: 135-43. PMID 12664266 DOI: 10.1007/s00792-002-0305-4  1
2003 Aghajanian S, Hovsepyan M, Geoghegan KF, Chrunyk BA, Engel PC. A thermally sensitive loop in clostridial glutamate dehydrogenase detected by limited proteolysis. The Journal of Biological Chemistry. 278: 1067-74. PMID 12414808 DOI: 10.1074/jbc.M206099200  1
2002 Seah SY, Britton KL, Rice DW, Asano Y, Engel PC. Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates. Biochemistry. 41: 11390-7. PMID 12234181 DOI: 10.1021/bi020196a  1
2002 Wang XT, Au SW, Lam VM, Engel PC. Recombinant human glucose-6-phosphate dehydrogenase. Evidence for a rapid-equilibrium random-order mechanism. European Journal of Biochemistry / Febs. 269: 3417-24. PMID 12135480 DOI: 10.1046/j.1432-1033.2002.03015.x  1
2002 Hayden BM, Bonete MJ, Brown PE, Moir AJ, Engel PC. Glutamate dehydrogenase of Halobacterium salinarum: evidence that the gene sequence currently assigned to the NADP+-dependent enzyme is in fact that of the NAD+-dependent glutamate dehydrogenase. Fems Microbiology Letters. 211: 37-41. PMID 12052548 DOI: 10.1016/S0378-1097(02)00644-4  1
2001 Goyal A, Wang XG, Engel PC. Allosteric behaviour of 1:5 hybrids of mutant subunits of Clostridium symbiosum glutamate dehydrogenase differing in their amino acid specificity. The Biochemical Journal. 360: 651-6. PMID 11736656 DOI: 10.1042/0264-6021:3600651  1
2001 Wang XG, Britton KL, Stillman TJ, Rice DW, Engel PC. Conversion of a glutamate dehydrogenase into methionine/norleucine dehydrogenase by site-directed mutagenesis. European Journal of Biochemistry / Febs. 268: 5791-9. PMID 11722565 DOI: 10.1046/j.0014-2956.2001.02523.x  1
2001 Irwin JA, Alfredsson GA, Lanzetti AJ, Gudmundsson HM, Engel PC. Purification and characterisation of a serine peptidase from the marine psychrophile strain PA-43. Fems Microbiology Letters. 201: 285-90. PMID 11470375 DOI: 10.1016/S0378-1097(01)00292-0  1
2001 Irwin JA, Gudmundsson HM, Marteinsson VT, Hreggvidsson GO, Lanzetti AJ, Alfredsson GA, Engel PC. Characterization of alanine and malate dehydrogenases from a marine psychrophile strain PA-43. Extremophiles : Life Under Extreme Conditions. 5: 199-211. PMID 11453464 DOI: 10.1007/s007920100191  1
2001 Andresen BS, Dobrowolski SF, O'Reilly L, Muenzer J, McCandless SE, Frazier DM, Udvari S, Bross P, Knudsen I, Banas R, Chace DH, Engel P, Naylor EW, Gregersen N. Medium-chain acyl-CoA dehydrogenase (MCAD) mutations identified by MS/MS-based prospective screening of newborns differ from those observed in patients with clinical symptoms: Identification and characterization of a new, prevalent mutation that results in mild MCAD deficiency American Journal of Human Genetics. 68: 1408-1418. PMID 11349232 DOI: 10.1086/320602  1
2001 Coughlan S, Wang XG, Britton KL, Stillman TJ, Rice DW, Chiaraluce R, Consalvi V, Scandurra R, Engel PC. Contribution of an aspartate residue, D114, in the active site of clostridial glutamate dehydrogenase to the enzyme's unusual pH dependence. Biochimica Et Biophysica Acta. 1544: 10-7. PMID 11341912 DOI: 10.1016/S0167-4838(00)00200-4  1
2001 Hayden BM, Engel PC. Construction, separation and properties of hybrid hexamers of glutamate dehydrogenase in which five of the six subunits are contributed by the catalytically inert D165S. European Journal of Biochemistry / Febs. 268: 1173-80. PMID 11231268 DOI: 10.1046/j.1432-1327.2001.01949.x  1
2000 Scandurra R, Consalvi V, Chiaraluce R, Politi L, Engel PC. Protein stability in extremophilic archaea. Frontiers in Bioscience : a Journal and Virtual Library. 5: D787-95. PMID 10966879  1
2000 Parker A, Engel PC. Preliminary evidence for the existence of specific functional assemblies between enzymes of the beta-oxidation pathway and the respiratory chain. The Biochemical Journal. 345: 429-35. PMID 10642498 DOI: 10.1042/0264-6021:3450429  1
1999 Udvari S, Bross P, Andresen BS, Gregersen N, Engel PC. Biochemical characterisation of mutations of human medium-chain acyl-CoA dehydrogenase. Advances in Experimental Medicine and Biology. 466: 387-93. PMID 10709667  1
1999 Parker A, Engel PC. Formation of a human "electron transferring flavoprotein". Medium chain acyl coenzyme A dehydrogenase complex, preliminary evidence from crosslinking studies. Advances in Experimental Medicine and Biology. 466: 191-4. PMID 10709643  1
1999 Parker A, Engel PC. 5,5'-Dithiobis-(2-nitrobenzoic acid) as a probe for a non-essential cysteine residue at the medium chain acyl-coenzyme A dehydrogenase binding site of the human 'electron transferring flavoprotein' (ETF). Journal of Enzyme Inhibition. 14: 381-90. PMID 10488248  1
1999 Hayden BM, Dean JL, Martin SR, Engel PC. Chemical rescue of the catalytically disabled clostridial glutamate dehydrogenase mutant D165S by fluoride ion. The Biochemical Journal. 340: 555-60. PMID 10333502 DOI: 10.1042/0264-6021:3400555  1
1999 Aghajanian S, Walsh TP, Engel PC. Specificity of coenzyme analogues and fragments in promoting or impeding the refolding of clostridial glutamate dehydrogenase. Protein Science : a Publication of the Protein Society. 8: 866-72. PMID 10211832 DOI: 10.1110/ps.8.4.866  1
1999 Stillman TJ, Migueis AM, Wang XG, Baker PJ, Britton KL, Engel PC, Rice DW. Insights into the mechanism of domain closure and substrate specificity of glutamate dehydrogenase from Clostridium symbiosum. Journal of Molecular Biology. 285: 875-85. PMID 9878450 DOI: 10.1006/jmbi.1998.2335  1
1998 Parker A, Engel PC. Experimental evidence for the formation of a complex between butyryl coenzyme A dehydrogenase and the 'electron transferring flavoprotein', ETF. Biochemical Society Transactions. 26: S73. PMID 10909831  1
1998 Irwin JA, Alfredsson GA, Gudmundsson HM, Engel PC. Purification and properties of psychrophilic dehydrogenases. Biochemical Society Transactions. 26: S72. PMID 10909830  1
1998 Udvari S, Bross P, Andresen BS, Gregersen N, Engel PC. Mutations of human medium-chian acyl-CoA dehydrogenase. Biochemical Society Transactions. 26: S65. PMID 10909823  1
1998 Goyal A, Aghajanian S, Hayden BM, Wang XG, Engel PC. Construction and investigation of co-operativity in hybrids of norleucine- and glutamate-active subunits of clostridial glutamate dehydrogenase. Biochemical Society Transactions. 26: S27. PMID 10909785  1
1998 Aghajanian S, Hovsepyan M, Geoghegan KF, Chrunyk BA, Engel PC. Probing the stability of the tertiary structure of glutamate dehydrogenase by limited proteolysis. Biochemical Society Transactions. 26: S26. PMID 10909784  1
1998 Goyal A, Wang XG, Engel PC. Patterns of activation of the norleucine activity of a quadruple mutant derived from clostridial glutamate dehydrogenase. Biochemical Society Transactions. 26: S380. PMID 10047894  1
1998 Scandurra R, Consalvi V, Chiaraluce R, Politi L, Engel PC. Protein thermostability in extremophiles. Biochimie. 80: 933-41. PMID 9893953 DOI: 10.1016/S0300-9084(00)88890-2  1
1998 Broadway NM, Engel PC. Novel methylenecyclopropyl-based acyl-CoA dehydrogenase inhibitor. Febs Letters. 437: 122-6. PMID 9804184 DOI: 10.1016/S0014-5793(98)01155-7  1
1998 Parker A, Engel PC. Chemical modification of histidine residues in human 'electron transferring flavoprotein' (ETF). Biochemical Society Transactions. 26: S272. PMID 9765991  1
1998 Aghajanian S, Engel PC. Use of protein engineering to explore subunit interactions in an allosteric enzyme: construction of inter-subunit hybrids in Clostridium symbiosum glutamate dehydrogenase. Protein Engineering. 11: 569-75. PMID 9740375  1
1998 Millevoi S, Pasquo A, Chiaraluce R, Consalvi V, Giangiacomo L, Britton KL, Stillman TJ, Rice DW, Engel PC. A monomeric mutant of Clostridium symbiosum glutamate dehydrogenase: comparison with a structured monomeric intermediate obtained during refolding. Protein Science : a Publication of the Protein Society. 7: 966-74. PMID 9568903 DOI: 10.1002/pro.5560070414  1
1998 Basso LA, Engel PC, Walmsley AR. Cooperativity between trimers of the hexameric glutamate dehydrogenase from Clostridium symbiosum. Biochimica Et Biophysica Acta. 1382: 345-50. PMID 9540807 DOI: 10.1016/S0167-4838(97)00107-6  1
1998 Britton KL, Stillman TJ, Yip KS, Forterre P, Engel PC, Rice DW. Insights into the molecular basis of salt tolerance from the study of glutamate dehydrogenase from Halobacterium salinarum. The Journal of Biological Chemistry. 273: 9023-30. PMID 9535890 DOI: 10.1074/jbc.273.15.9023  1
1998 Etchegaray A, Dieckmann R, Engel PC, Turner G, von Döhren H. Group specific antibodies against the putative AMP-binding domain signature SGTTGXPKG in peptide synthetases and related enzymes. Biochemistry and Molecular Biology International. 44: 235-43. PMID 9530507  1
1997 Baker PJ, Waugh ML, Wang XG, Stillman TJ, Turnbull AP, Engel PC, Rice DW. Determinants of substrate specificity in the superfamily of amino acid dehydrogenases. Biochemistry. 36: 16109-15. PMID 9405044 DOI: 10.1021/bi972024x  1
1997 Goyal A, Aghajanian S, Hayden BM, Wang XG, Engel PC. Intersubunit communication in hybrid hexamers of K89L/A163G/S380A and C320S mutants of glutamate dehydrogenase from Clostridium symbiosum. Biochemistry. 36: 15000-5. PMID 9398225 DOI: 10.1021/bi971419d  1
1997 Aghajanian S, Engel PC. Re-activation of Clostridium symbiosum glutamate dehydrogenase from subunits denatured by urea. The Biochemical Journal. 326: 649-55. PMID 9307012  1
1997 Basso LA, Engel PC, Walmsley AR. Kinetic studies on the binding of 1,N6-etheno-NAD+ to glutamate dehydrogenase from Clostridium symbiosum. Biochimica Et Biophysica Acta. 1340: 63-71. PMID 9217015 DOI: 10.1016/S0167-4838(97)00032-0  1
1997 Dean JL, Cölfen H, Harding SE, Rice DW, Engel PC. Alteration of the quaternary structure of glutamate dehydrogenase from Clostridium symbiosum by a single mutation distant from the subunit interfaces. European Biophysics Journal : Ebj. 25: 417-22. PMID 9188163 DOI: 10.1007/s002490050055  1
1997 Aghajanian SA, Engel PC. The role and importance of different fragments of NAD+ in refolding of Clostridium symbiosum glutamate dehydrogenase. Biochemical Society Transactions. 25: 56S. PMID 9056954  1
1996 Pasquo A, Britton KL, Stillman TJ, Rice DW, Cölfen H, Harding SE, Scandurra R, Engel PC. Construction of a dimeric form of glutamate dehydrogenase from Clostridium symbiosum by site-directed mutagenesis. Biochimica Et Biophysica Acta. 1297: 149-58. PMID 8917616 DOI: 10.1016/S0167-4838(96)00017-9  1
1996 Coughlan S, Wang XG, Britton KL, Stillman TJ, Rice DW, Engel PC. Preliminary functional studies of mutant D114N of Clostridium symbiosum glutamate dehydrogenase. Biochemical Society Transactions. 24: 149S. PMID 8674642  1
1996 Aghajanian SA, Wang XG, Engel PC. In vitro construction of inter-subunit hybrids in Clostridium symbiosum glutamate dehydrogenase. Biochemical Society Transactions. 24: 136S. PMID 8674626  1
1996 Wang XG, Britton LK, Baker PJ, Rice DW, Engel PC. Improving the engineered activity of mutants of clostridial glutamate dehydrogenase towards monocarboxylic substrates: substitution of Ala163 with glycine. Biochemical Society Transactions. 24: 126S. PMID 8674611  0.48
1996 Rice DW, Yip KS, Stillman TJ, Britton KL, Fuentes A, Connerton I, Pasquo A, Scandura R, Engel PC. Insights into the molecular basis of thermal stability from the structure determination of Pyrococcus furiosus glutamate dehydrogenase. Fems Microbiology Reviews. 18: 105-17. PMID 8639325 DOI: 10.1016/0168-6445(96)00005-8  1
1995 Yip KS, Baker PJ, Britton KL, Engel PC, Rice DW, Sedelnikova SE, Stillman TJ, Pasquo A, Chiaraluce R, Consalvi V, Scandurra R. Crystallization of the NAD(P)-dependent glutamate dehydrogenase from the hyperthermophile Pyrococcus furiosus. Acta Crystallographica. Section D, Biological Crystallography. 51: 240-2. PMID 15299326 DOI: 10.1107/S0907444994012862  0.48
1995 Yip KS, Stillman TJ, Britton KL, Artymiuk PJ, Baker PJ, Sedelnikova SE, Engel PC, Pasquo A, Chiaraluce R, Consalvi V. The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures. Structure (London, England : 1993). 3: 1147-58. PMID 8591026 DOI: 10.1016/S0969-2126(01)00251-9  0.48
1995 Basso LA, Engel PC, Walmsley AR. The mechanism of substrate and coenzyme binding to clostridial glutamate dehydrogenase during reductive amination. European Journal of Biochemistry / Febs. 234: 603-15. PMID 8536710  1
1995 Britton KL, Baker PJ, Borges KM, Engel PC, Pasquo A, Rice DW, Robb FT, Scandurra R, Stillman TJ, Yip KS. Insights into thermal stability from a comparison of the glutamate dehydrogenases from Pyrococcus furiosus and Thermococcus litoralis. European Journal of Biochemistry / Febs. 229: 688-95. PMID 7758464  0.48
1995 Teller JK, Baker PJ, Britton KL, Engel PC, Rice DW, Stillman TJ. Correlation of intron-exon organisation with the three-dimensional structure in glutamate dehydrogenase. Biochimica Et Biophysica Acta. 1247: 231-8. PMID 7696313 DOI: 10.1016/0167-4838(94)00240-H  1
1995 Seah SY, Britton KL, Baker PJ, Rice DW, Asano Y, Engel PC. Alteration in relative activities of phenylalanine dehydrogenase towards different substrates by site-directed mutagenesis. Febs Letters. 370: 93-6. PMID 7649311 DOI: 10.1016/0014-5793(95)00804-I  1
1995 Wang XG, Britton KL, Baker PJ, Martin S, Rice DW, Engel PC. Alteration of the amino acid substrate specificity of clostridial glutamate dehydrogenase by site-directed mutagenesis of an active-site lysine residue. Protein Engineering. 8: 147-52. PMID 7630885 DOI: 10.1093/protein/8.2.147  1
1995 Wang XG, Engel PC. Positive cooperativity with Hill coefficients of up to 6 in the glutamate concentration dependence of steady-state reaction rates measured with clostridial glutamate dehydrogenase and the mutant A163G at high pH. Biochemistry. 34: 11417-22. PMID 7547869  1
1995 Aghajanian SA, Martin SR, Engel PC. Urea-induced inactivation and denaturation of clostridial glutamate dehydrogenase: the absence of stable dimeric or trimeric intermediates. The Biochemical Journal. 311: 905-10. PMID 7487949  1
1994 Syed SE, Hornby DP, Brown PE, Fitton JE, Engel PC. Site and significance of chemically modifiable cysteine residues in glutamate dehydrogenase of Clostridium symbiosum and the use of protection studies to measure coenzyme binding. The Biochemical Journal. 298: 107-13. PMID 8129708  1
1994 Dean JL, Wang XG, Teller JK, Waugh ML, Britton KL, Baker PJ, Stillman TJ, Martin SR, Rice DW, Engel PC. The catalytic role of aspartate in the active site of glutamate dehydrogenase. The Biochemical Journal. 301: 13-6. PMID 8037659  0.48
1994 Cadenas Q, Engel PC. Activity staining of halophilic enzymes: Substitution of salt with a zwitterion in non-denaturing electrophoresis Biochemistry and Molecular Biology International. 33: 785-792. PMID 7981666  1
1994 Wang XG, Dean JL, Engel PC, Baker PJ, Britton KL, Stillman TJ, Rice DW. The changed pattern of substrate specificity in the K89L mutant of glutamate dehydrogenase of Clostridium symbiosum. Biochemical Society Transactions. 22: 320S. PMID 7821579  0.48
1994 Basso LA, Engel PC, Walmsley AR. Steady-state kinetics and transient studies of substrate and coenzyme analogue binding to clostridial glutamate dehydrogenase (GDH) during oxidative deamination Biochemical Society Transactions. 22: 319S. PMID 7821578  1
1994 Aghajanian SA, Engel PC. Studies of dissociation-association processes in clostridial glutamate dehydrogenase Biochemical Society Transactions. 22: 318S. PMID 7821577  1
1994 Basso LA, Engel PC. Initial formation of a non-covalent enzyme-reagent complex during the inactivation of clostridial glutamate dehydrogenase by Ellman's reagent: determination of the enzyme's dissociation constant for the binary complex with NAD+ from protection studies. Biochimica Et Biophysica Acta. 1209: 222-6. PMID 7811694 DOI: 10.1016/0167-4838(94)90188-0  1
1994 Wang XG, Engel PC. Identification of the reactive cysteine in clostridial glutamate dehydrogenase by site-directed mutagenesis and proof that this residue is not strictly essential Protein Engineering. 7: 1013-1016. PMID 7809027  1
1993 Ellison PA, Engel PC. Intrinsic crotonase activity in a bacterial butyryl-CoA dehydrogenase. Biochemistry and Molecular Biology International. 29: 605-12. PMID 8490573  1
1993 Syed SEH, Engel PC. Inhibition of glutamate dehydrogenase by covalent coenzyme-substrate adducts: A re-examination Biochemistry and Molecular Biology International. 30: 283-291. PMID 8364410  1
1993 Britton KL, Baker PJ, Engel PC, Rice DW, Stillman TJ. Evolution of substrate diversity in the superfamily of amino acid dehydrogenases: Prospects for rational chiral synthesis Journal of Molecular Biology. 234: 938-945. PMID 8263939  1
1993 Korber FC, Rizkallah PJ, Attwood TK, Wootton JC, McPherson MJ, North AC, Geddes AJ, Abeysinghe IS, Baker PJ, Dean JL. Crystallization of the NADP(+)-dependent glutamate dehydrogenase from Escherichia coli. Journal of Molecular Biology. 234: 1270-3. PMID 8263929 DOI: 10.1006/jmbi.1993.1680  1
1993 Basso LA, Engel PC, Walmsley AR. The mechanism of substrate and coenzyme binding to clostridial glutamate dehydrogenase during oxidative deamination European Journal of Biochemistry. 213: 935-945. PMID 8099328 DOI: 10.1111/j.1432-1033.1993.tb17838.x  1
1992 Lilley KS, Engel PC. The essential active-site lysines of clostridial glutamate dehydrogenase. A study with pyridoxal-5'-phosphate European Journal of Biochemistry. 207: 533-540. PMID 1633808 DOI: 10.1111/j.1432-1033.1992.tb17079.x  1
1992 Baker PJ, Britton KL, Engel PC, Farrants GW, Lilley KS, Rice DW, Stillman TJ. Subunit assembly and active site location in the structure of glutamate dehydrogenase Proteins: Structure, Function and Genetics. 12: 75-86. PMID 1553382  0.48
1992 Curtis D, Blakemore AI, Engel PC, Kolvraa S, Gregersen N, Pollitt RJ. Medium chain acyl-CoA dehydrogenase deficiency in the United Kingdom Progress in Clinical and Biological Research. 375: 489-494. PMID 1438392  1
1991 Lilley KS, Baker PJ, Britton KL, Stillman TJ, Brown PE, Moir AJ, Engel PC, Rice DW, Bell JE, Bell E. The partial amino acid sequence of the NAD(+)-dependent glutamate dehydrogenase of Clostridium symbiosum: implications for the evolution and structural basis of coenzyme specificity. Biochimica Et Biophysica Acta. 1080: 191-7. PMID 1954226 DOI: 10.1016/0167-4838(91)90001-G  1
1991 Gregersen N, Blakemore AIF, Winter V, Andresen B, Kølvraa S, Bolund L, Curtis D, Engel PC. Specific diagnosis of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency in dried blood spots by a polymerase chain reaction (PCR) assay detecting a point-mutation (G985) in the MCAD gene Clinica Chimica Acta. 203: 23-34. PMID 1769118 DOI: 10.1016/0009-8981(91)90153-4  1
1991 Syed SEH, Engel PC, Parker DM. Functional studies of a glutamate dehydrogenase with known three-dimensional structure: steady-state kinetics of the forward and reverse reactions catalysed by the NAD+-dependent glutamate dehydrogenase of Clostridium symbiosum Bba - General Subjects. 1115: 123-130. PMID 1764463 DOI: 10.1016/0304-4165(91)90020-H  1
1991 Curtis D, Blakemore AIF, Engel PC, Macgregor D, Besley G, Kolvraa S, Gregersen N. Heterogeneity for mutations in medium chain acyl-CoA dehydrogenase deficiency in the UK population Clinical Genetics. 40: 283-286. PMID 1756601  1
1991 Kølvraa S, Gregersen N, Blakemore AIF, Schneidermann AK, Winter V, Andresen BS, Curtis D, Engel PC, Pricille D, Rhead W, Bolund L. The most common mutation causing medium-chain acyl-CoA dehydrogenase deficiency is strongly associated with a particular haplotype in the region of the gene Human Genetics. 87: 425-428. PMID 1679031 DOI: 10.1007/BF00197161  1
1991 Blakemore AIF, Kolvraa S, Gregersen N, Engel PC, Curtis D. Localisation of RFLPs of the medium chain acyl-CoA dehydrogenase gene Human Genetics. 86: 537-538. PMID 1673116 DOI: 10.1007/BF00194652  1
1990 Syed SEH, Engel PC. A pH-dependent activation-inactivation equilibrium in glutamate dehydrogenase of Clostridium symbiosum Biochemical Journal. 271: 351-355. PMID 2241920  1
1990 Blakemore AIF, Engel PC, Curtis D. PstI RFLP at the medium chain acyl-Coenzyme A dehydrogenase locus (ACADM chromosome 1) Nucleic Acids Research. 18: 2838. PMID 1971104  1
1990 Blakemore AIF, Engel PC, Curtis D. BamHI and MspI RFLP's in strong linkage disequilibrium at the medium chain acyl-Coenzyme A dehydrogenase locus (ACADM chromosome 1) Nucleic Acids Research. 18: 2838. PMID 1692618  1
1990 Syed SEH, Engel PC, Martin SR. A circular dichroism study of the pH-dependent activation/inactivation equilibrium in the glutamate dehydrogenase of Clostridium symbiosum Febs Letters. 262: 176-178. DOI: 10.1016/0014-5793(90)80183-J  1
1988 Allison F, Bennett MJ, Variend S, Engel PC. Acylcoenzyme A dehydrogenase deficiency in heart tissue from infants who died unexpectedly with fatty change in the liver. British Medical Journal (Clinical Research Ed.). 296: 11-2. PMID 3122912  1
1987 Shaw L, Engel PC. CoA-persulphide: a possible in vivo inhibitor of mammalian short-chain acyl-CoA dehydrogenase Biochimica Et Biophysica Acta (Bba)/Lipids and Lipid Metabolism. 919: 171-174. PMID 3580384 DOI: 10.1016/0005-2760(87)90204-9  1
1985 Shaw L, Engel PC. The suicide inactivation of ox liver short-chain acyl-CoA dehydrogenase by propionyl-CoA. Formation of an FAD adduct Biochemical Journal. 230: 723-731. PMID 4062874  1
1985 Hornby DP, Engel PC, Hatanaka S. Kinetic studies of ox-liver glutamate dehydrogenase oxidative deamination of two glutamate analogues, L-threo-gamma-methylglutamate and L-alpha-amino-gamma-nitraminobutyrate, in the presence of the allosteric effector ADP. The International Journal of Biochemistry. 17: 851-4. PMID 4054426 DOI: 10.1016/0020-711X(85)90277-0  1
1985 Rice DW, Hornby DP, Engel PC. Crystallization of an NAD+-dependent glutamate dehydrogenase from Clostridium symbiosum. Journal of Molecular Biology. 181: 147-9. PMID 3981633 DOI: 10.1016/0022-2836(85)90334-1  1
1985 Howat AJ, Bennett MJ, Variend S, Shaw L, Engel PC. Defects of metabolism of fatty acids in the sudden infant death syndrome British Medical Journal. 290: 1771-1773. PMID 3924247  1
1984 Williamson G, Engel PC. Butyryl-CoA dehydrogenase from Megasphaera elsdenii. Specificity of the catalytic reaction Biochemical Journal. 218: 521-529. PMID 6712628  1
1984 Shaw L, Engel PC. The purification and properties of ox liver short-chain acyl-CoA dehydrogenase Biochemical Journal. 218: 511-520. PMID 6712627  1
1984 Hornby DP, Engel PC. Characterization of Peptostreptococcus asaccharolyticus glutamate dehydrogenase purified by dye-ligand chromatography. Journal of General Microbiology. 130: 2385-94. PMID 6502134  1
1984 Bennett MJ, Curnock DA, Engel PC, Shaw L, Gray RGF, Hull D, Patrick AD, Pollitt RJ. Glutaric aciduria type II: Biochemical investigation and treatment of a child diagnosed prenatally Journal of Inherited Metabolic Disease. 7: 57-61. PMID 6207379 DOI: 10.1007/BF01805802  1
1984 Hornby DP, Aitchison MJ, Engel PC. The kinetic mechanism of ox liver glutamate dehydrogenase in the presence of the allosteric effector ADP. The oxidative deamination of L-glutamate. The Biochemical Journal. 223: 161-8. PMID 6149744  1
1984 Syed SE, Engel PC. Ox liver glutamate dehydrogenase. The use of chemical modification to study the relationship between catalytic sites for different amino acid substrates and the question of kinetic non-equivalence of the subunits Biochemical Journal. 222: 621-626. PMID 6148932  1
1984 Hornby DP, Engel PC. A kinetic study of the oxidative deamination of L-glutamate by Peptostreptococcus asaccharolyticus glutamate dehydrogenase using a variety of coenzymes. European Journal of Biochemistry / Febs. 143: 557-60. PMID 6148240  1
1984 Engel PC. Chapter 3 Enzyme kinetics New Comprehensive Biochemistry. 6: 73-110. DOI: 10.1016/S0167-7306(08)60374-9  1
1983 Williamson G, Engel PC. The effect on butyryl-CoA dehydrogenase of reagents specific for nucleophilic sulphur Biochemical Journal. 211: 559-566. PMID 6882359  1
1983 Hornby DP, Engel PC, Hatanaka S. Beef liver glutamate dehydrogenase: a study of the oxidation of various alternative amino acid substrates retaining the correct spacing of the two carboxylate groups. The International Journal of Biochemistry. 15: 495-500. PMID 6852348 DOI: 10.1016/0020-711X(83)90122-2  1
1983 Aitchison MJ, Engel PC. Beef liver glutamate dehydrogenase : Effects of partial proteolysis with chymotrypsin International Journal of Biochemistry. 15: 79-85. PMID 6825906 DOI: 10.1016/0020-711X(83)90014-9  1
1982 Williamson G, Engel PC. A convenient and rapid method for the complete removal of CoA from butyryl-CoA dehydrogenase Biochimica Et Biophysica Acta (Bba)/Protein Structure and Molecular. 706: 245-248. PMID 7126602 DOI: 10.1016/0167-4838(82)90493-9  1
1982 Williamson G, Engel PC, Mizzer JP, Thorpe C, Massey V. Evidence that the greening ligand in native butyryl-CoA dehydrogenase is a CoA persulfide Journal of Biological Chemistry. 257: 4314-4320. PMID 7068637  1
1982 Williamson G, Engel PC, Nishina Y, Shiga K. A resonance Raman study on the nature of charge-transfer interactions in butyryl CoA dehydrogenase. Febs Letters. 138: 29-32. PMID 7067828 DOI: 10.1016/0014-5793(82)80387-6  1
1982 Gould KG, Engel PC. The reactions of pyridoxal 5′-phosphate with the M4 and H4 isoenzymes of pig lactate dehydrogenase Archives of Biochemistry and Biophysics. 215: 498-507. PMID 6807212 DOI: 10.1016/0003-9861(82)90108-4  1
1981 Engel PC. [43] Butyryl-CoA dehydrogenase from Megasphaera elsdenii. EC 1.3.99.2 butyryl-CoA:(acceptor) oxidoreductase Methods in Enzymology. 71: 359-366. DOI: 10.1016/0076-6879(81)71045-0  1
1980 Aitchison MJ, Engel PC. The effects of digestion by bovine pancreas alpha-chymotrypsin on native bovine glutamate dehydrogenase Biochemical Society Transactions. 8: 649. PMID 7450274  1
1980 Gould KG, Engel PC. Modification of mouse testicular lactate dehydrogenase by pyridoxal 5'-phosphate Biochemical Journal. 191: 365-371. PMID 6786279  1
1979 Gould KG, Engel PC. A re-examination of the reaction between Ox liver glutamate dehydrogenase and 1-fluoro-2,4-dinitrobenzene Archives of Biochemistry and Biophysics. 196: 284-294. PMID 41482 DOI: 10.1016/0003-9861(79)90578-2  1
1978 Gould KG, Engel PC. Desensitization of bovine liver glutamate dehydrogenase by 1-fluoro-2,4-dinitrobenzene Biochemical Society Transactions. 6: 1226-1228. PMID 744394  1
1978 Engel PC. The detection of amino acid sequence homology: A new scoring method based on the genetic code with allowance for redundancy Journal of Theoretical Biology. 72: 1-8. PMID 661329 DOI: 10.1016/0022-5193(78)90014-0  1
1978 Engel PC. Fatty acyl-coenzyme A dehydrogenases Biochemical Society Transactions. 6: 76-80. PMID 640208  1
1978 Engel PC, Jones JB. Causes and elimination of erratic blanks in enzymatic metabolite assays involving the use of NAD+ in alkaline hydrazine buffers: Improved conditions for the assay of l-glutamate, l-lactate, and other metabolites Analytical Biochemistry. 88: 475-484. PMID 29519 DOI: 10.1016/0003-2697(78)90447-5  1
1978 Engel PC, Jones JB. A reappraisal of the reaction of butyryl-coenzyme A dehydrogenase with phenylmercuric acetate. Evidence that de-greening involves a reaction of the tightly bound thioester Biochemical Journal. 171: 51-59. PMID 25661  1
1977 Engel PC. Problems in the application of gel filtration to the desalting of organic compounds: Retardation of aromatic and heteroaromatic anions by commonly used salts Analytical Biochemistry. 82: 512-522. PMID 199084 DOI: 10.1016/0003-2697(77)90190-7  1
1977 Chen SS, Engel PC, Bayley PM. The allosteric mechanism of bovine liver glutamate dehydrogenase. Evidence from circular dichroism studies for a conformational change in the ternary complex enzyme (oxidized nicotinamide adenine dinucleotide) glutarate Biochemical Journal. 163: 297-302. PMID 194583  1
1977 Dickinson FM, Engel PC. The preparation of pure salt-free nicotinamide coenzymes Analytical Biochemistry. 82: 523-531. PMID 20816 DOI: 10.1016/0003-2697(77)90191-9  1
1975 Chen SS, Engel PC. Modification of pig M4 lactate dehydrogenase by pyridoxal 5 phosphate. Demonstration of an essential lysine residue Biochemical Journal. 149: 107-113. PMID 1238085  1
1975 Chen SS, Engel PC. The equilibrium position of the reaction of bovine liver glutamate dehydrogenase with pyridoxal 5' phosphate. A demonstration that covalent modification with this reagent completely abolishes catalytic activity Biochemical Journal. 147: 351-358. PMID 1237292  1
1975 Engel PC, Chen SS. The nature of inhibition and inactivation of bovine liver glutamate dehydrogenase by pyridoxal 5' phosphate Biochemical Society Transactions. 3: 78-80. PMID 1126546  1
1975 Chen SS, Engel PC. Dogfish M4 lactate dehydrogenase: reversible inactivation by pyridoxal 5' phosphate and complete protection in complexes that mimic the active ternary complex Biochemical Journal. 151: 447-449. PMID 175780  1
1975 Engel PC, Chen SS. A product inhibition study of bovine liver glutamate dehydrogenase Biochemical Journal. 151: 305-318. PMID 175778  1
1975 Chen SS, Engel PC. Reversible modification of pig heart mitochondrial malate dehydrogenase by pyridoxal 5' phosphate Biochemical Journal. 151: 297-303. PMID 175777  1
1975 Chen SS, Engel PC. Horse liver alcohol dehydrogenase. A study of the essential lysine residue Biochemical Journal. 149: 627-635. PMID 173294  1
1975 Chen SS, Engel PC. Ox liver glutamate dehydrogenase. The role of lysine 126 reappraised in the light of studies of inhibition and inactivation by pyridoxal 5' phosphate Biochemical Journal. 149: 619-626. PMID 173293  1
1975 Chen SS, Engel PC. Inactivation of nicotinamide adenine dinucleotide linked dehydrogenases by pyridoxal 5' phosphate Biochemical Society Transactions. 3: 80-82. PMID 165109  1
1975 Chen SS, Engel PC. Equilibrium protection studies of the interaction of bovine glutamate dehydrogenase with purine nucleotide effectors Febs Letters. 58: 202-206. PMID 5298 DOI: 10.1016/0014-5793(75)80259-6  1
1974 Engel PC. Possibility of inborn defect in isovalericacidaemia involving altered enzyme specificity rather than total inactivity Nature. 248: 140-142. PMID 4818917  1
1974 Chen SS, Engel PC. Protection of glutamate dehydrogenase by nicotinamide adenine dinucleotide against reversible inactivation by pyridoxal 5' phosphate as a sensitive indicator of conformational change induced by substrates and substrate analogues Biochemical Journal. 143: 569-574. PMID 4376949  1
1974 Engel PC. On the evidence for partial gene duplication from amino acid sequence of bovine glutamate dehydrogenase (reply) Nature. 247: 557. DOI: 10.1038/247557a0  1
1973 Engel PC. Evolution of enzyme regulator sites: Evidence for partial gene duplication from amino-acid sequence of bovine glutamate dehydrogenase [11] Nature. 241: 118-120. PMID 4695538 DOI: 10.1038/241118a0  1
1973 Engel PC. Sequence homologies among pyridine nucleotide-linked dehydrogenases: Possible partial gene duplications in glyceraldehyde-3-phosphate dehydrogenase Febs Letters. 33: 151-153. PMID 4578953 DOI: 10.1016/0014-5793(73)80181-4  1
1973 Engel PC, Ferdinand W. The significance of abrupt transitions in Lineweaver-Burk plots with particular reference to glutamate dehydrogenase. Negative and positive co-operativity in catalytic rate constants Biochemical Journal. 131: 97-105. PMID 4352866  1
1972 Engel PC. The interpretation of abrupt transitions in Lineweaver-Burk plots Biochemical Journal. 130: 42P-43P. PMID 4655449  1
1971 Engel PC, Massey V. Green butyryl-coenzyme A dehydrogenase. An enzyme-acyl-coenzyme A complex Biochemical Journal. 125: 889-902. PMID 5145911  1
1971 Engel PC, Massey V. The purification and properties of butyryl-coenzyme A dehydrogenase from Peptostreptococcus elsdenii Biochemical Journal. 125: 879-887. PMID 5145910  1
1970 Engel PC, Dalziel K. Kinetic studies of glutamate dehydrogenase. The reductive amination of 2-oxoglutarate. The Biochemical Journal. 118: 409-19. PMID 4394334  1
1969 Massey V, Strickland S, Mayhew SG, Howell LG, Engel PC, Matthews RG, Schuman M, Sullivan PA. The production of superoxide anion radicals in the reaction of reduced flavins and flavoproteins with molecular oxygen. Biochemical and Biophysical Research Communications. 36: 891-7. PMID 5388670  1
1969 Engel PC, Dalziel K. Kinetic studies of glutamate dehydrogenase with glutamate and norvaline as substrates. Coenzyme activation and negative homotropic interactions in allosteric enzymes. The Biochemical Journal. 115: 621-31. PMID 4391040  1
1968 Dalziel K, Engel PC. Antagonistic homotropic interactions as a possible explanation of coenzyme activation of glutamate dehydrogenase. Febs Letters. 1: 349-352. PMID 11945341  1
1967 Engel PC, Dalziel K. The equilibrium constants of the glutamate dehydrogenase systems. The Biochemical Journal. 105: 691-5. PMID 4384597  1
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