James R. Halpert - Publications

Pharmacy University of California, San Diego, La Jolla, CA 

59 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2018 Shah MB, Zhang Q, Halpert JR. Crystal Structure of CYP2B6 in Complex with an Efavirenz Analog. International Journal of Molecular Sciences. 19. PMID 29596329 DOI: 10.3390/Ijms19041025  0.326
2016 Liu J, Shah MB, Zhang Q, Stout CD, Halpert JR, Wilderman PR. Coumarin Derivatives as Substrate Probes of Mammalian Cytochromes P450 2B4 and 2B6: Assessing the Importance of 7-Alkoxy Chain Length, Halogen Substitution, and Non-Active Site Mutations. Biochemistry. PMID 26982502 DOI: 10.1021/Acs.Biochem.5B01330  0.334
2015 Jang HH, Liu J, Lee GY, Halpert JR, Wilderman PR. Functional importance of a peripheral pocket in mammalian cytochrome P450 2B enzymes. Archives of Biochemistry and Biophysics. 584: 61-69. PMID 26319176 DOI: 10.1016/j.abb.2015.08.007  0.319
2015 Shah MB, Wilderman PR, Liu J, Jang HH, Zhang Q, Stout CD, Halpert JR. Structural and biophysical characterization of human cytochromes P450 2B6 and 2A6 bound to volatile hydrocarbons: analysis and comparison. Molecular Pharmacology. 87: 649-59. PMID 25585967 DOI: 10.1124/Mol.114.097014  0.314
2015 Müller CS, Knehans T, Davydov DR, Bounds PL, von Mandach U, Halpert JR, Caflisch A, Koppenol WH. Concurrent cooperativity and substrate inhibition in the epoxidation of carbamazepine by cytochrome P450 3A4 active site mutants inspired by molecular dynamics simulations. Biochemistry. 54: 711-21. PMID 25545162 DOI: 10.1021/Bi5011656  0.306
2015 Davydov DR, Davydova NY, Sineva EV, Halpert JR. Interactions among cytochromes P450 in microsomal membranes: Oligomerization of cytochromes P4503A4,3A5, and 2E1 and its functional consequences Journal of Biological Chemistry. 290: 3850-3868. PMID 25533469 DOI: 10.1074/jbc.M114.615443  0.329
2014 Jang HH, Davydov DR, Lee GY, Yun CH, Halpert JR. The role of cytochrome P450 2B6 and 2B4 substrate access channel residues predicted based on crystal structures of the amlodipine complexes. Archives of Biochemistry and Biophysics. 545: 100-7. PMID 24445070 DOI: 10.1016/j.abb.2014.01.008  0.353
2014 Wilderman PR, Jang HH, Malenke JR, Salib M, Angermeier E, Lamime S, Dearing MD, Halpert JR. Functional characterization of cytochromes P450 2B from the desert woodrat Neotoma lepida. Toxicology and Applied Pharmacology. 274: 393-401. PMID 24361551 DOI: 10.1016/J.Taap.2013.12.005  0.33
2013 Wilderman PR, Shah MB, Jang HH, Stout CD, Halpert JR. Structural and thermodynamic basis of (+)-α-pinene binding to human cytochrome P450 2B6. Journal of the American Chemical Society. 135: 10433-40. PMID 23786449 DOI: 10.1021/ja403042k  0.329
2013 Davydov DR, Davydova NY, Sineva EV, Kufareva I, Halpert JR. Pivotal role of P450-P450 interactions in CYP3A4 allostery: The case of α-naphthoflavone Biochemical Journal. 453: 219-230. PMID 23651100 DOI: 10.1042/BJ20130398  0.307
2013 Shah MB, Jang HH, Zhang Q, David Stout C, Halpert JR. X-ray crystal structure of the cytochrome P450 2B4 active site mutant F297A in complex with clopidogrel: insights into compensatory rearrangements of the binding pocket. Archives of Biochemistry and Biophysics. 530: 64-72. PMID 23296089 DOI: 10.1016/J.Abb.2012.12.016  0.326
2013 Zhang H, Gay SC, Shah M, Foroozesh M, Liu J, Osawa Y, Zhang Q, Stout CD, Halpert JR, Hollenberg PF. Potent mechanism-based inactivation of cytochrome P450 2B4 by 9-ethynylphenanthrene: Implications for allosteric modulation of cytochrome P450 catalysis Biochemistry. 52: 355-364. PMID 23276288 DOI: 10.1021/Bi301567Z  0.627
2012 Shah MB, Wilderman PR, Pascual J, Zhang Q, Stout CD, Halpert JR. Conformational adaptation of human cytochrome P450 2B6 and rabbit cytochrome P450 2B4 revealed upon binding multiple amlodipine molecules. Biochemistry. 51: 7225-38. PMID 22909231 DOI: 10.1021/Bi300894Z  0.316
2012 Davydov DR, Rumfeldt JAO, Sineva EV, Fernando H, Davydova NY, Halpert JR. Peripheral ligand-binding site in cytochrome P450 3A4 located with fluorescence resonance energy transfer (FRET) Journal of Biological Chemistry. 287: 6797-6809. PMID 22194603 DOI: 10.1074/jbc.M111.325654  0.325
2012 Wilderman PR, Gay SC, Jang HH, Zhang Q, Stout CD, Halpert JR. Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme. The Febs Journal. 279: 1607-20. PMID 22051155 DOI: 10.1111/J.1742-4658.2011.08411.X  0.654
2011 Shah MB, Pascual J, Zhang Q, Stout CD, Halpert JR. Structures of cytochrome P450 2B6 bound to 4-benzylpyridine and 4-(4-nitrobenzyl)pyridine: insight into inhibitor binding and rearrangement of active site side chains. Molecular Pharmacology. 80: 1047-55. PMID 21875942 DOI: 10.1124/Mol.111.074427  0.348
2011 Gay SC, Zhang H, Wilderman PR, Roberts AG, Liu T, Li S, Lin HL, Zhang Q, Woods VL, Stout CD, Hollenberg PF, Halpert JR. Structural analysis of mammalian cytochrome P450 2B4 covalently bound to the mechanism-based inactivator tert-butylphenylacetylene: insight into partial enzymatic activity. Biochemistry. 50: 4903-11. PMID 21510666 DOI: 10.1021/Bi200482G  0.631
2011 Halpert JR. Structure and function of cytochromes P450 2B: from mechanism-based inactivators to X-ray crystal structures and back. Drug Metabolism and Disposition: the Biological Fate of Chemicals. 39: 1113-21. PMID 21502194 DOI: 10.1124/dmd.111.039719  0.323
2010 Gay SC, Roberts AG, Halpert JR. Structural features of cytochromes P450 and ligands that affect drug metabolism as revealed by X-ray crystallography and NMR. Future Medicinal Chemistry. 2: 1451-68. PMID 21103389  0.615
2010 Gay SC, Roberts AG, Maekawa K, Talakad JC, Hong WX, Zhang Q, Stout CD, Halpert JR. Structures of cytochrome P450 2B4 complexed with the antiplatelet drugs ticlopidine and clopidogrel . Biochemistry. 49: 8709-20. PMID 20815363 DOI: 10.1021/Bi100914Z  0.634
2010 Gay SC, Shah MB, Talakad JC, Maekawa K, Roberts AG, Wilderman PR, Sun L, Yang JY, Huelga SC, Hong WX, Zhang Q, Stout CD, Halpert JR. Crystal structure of a cytochrome P450 2B6 genetic variant in complex with the inhibitor 4-(4-chlorophenyl)imidazole at 2.0-A resolution. Molecular Pharmacology. 77: 529-38. PMID 20061448 DOI: 10.1124/Mol.109.062570  0.633
2010 Talakad JC, Wilderman PR, Davydov DR, Kumar S, Halpert JR. Rational engineering of cytochromes P450 2B6 and 2B11 for enhanced stability: Insights into structural importance of residue 334. Archives of Biochemistry and Biophysics. 494: 151-8. PMID 19944064 DOI: 10.1016/j.abb.2009.11.026  0.312
2009 Gay SC, Sun L, Maekawa K, Halpert JR, Stout CD. Crystal structures of cytochrome P450 2B4 in complex with the inhibitor 1-biphenyl-4-methyl-1H-imidazole: ligand-induced structural response through alpha-helical repositioning. Biochemistry. 48: 4762-71. PMID 19397311 DOI: 10.1021/bi9003765  0.615
2009 Sun H, Moore C, Dansette PM, Kumar S, Halpert JR, Yost GS. Dehydrogenation of the indoline-containing drug 4-chloro-N-(2-methyl-1-indolinyl)-3-sulfamoylbenzamide (indapamide) by CYP3A4: correlation with in silico predictions. Drug Metabolism and Disposition: the Biological Fate of Chemicals. 37: 672-84. PMID 19074530 DOI: 10.1124/Dmd.108.022707  0.324
2008 Davydov DR, Halpert JR. Allosteric P450 mechanisms: multiple binding sites, multiple conformers or both? Expert Opinion On Drug Metabolism & Toxicology. 4: 1523-35. PMID 19040328 DOI: 10.1517/17425250802500028  0.312
2008 Oezguen N, Kumar S, Hindupur A, Braun W, Muralidhara BK, Halpert JR. Identification and analysis of conserved sequence motifs in cytochrome P450 family 2. Functional and structural role of a motif 187RFDYKD192 in CYP2B enzymes. The Journal of Biological Chemistry. 283: 21808-16. PMID 18495666 DOI: 10.1074/Jbc.M708582200  0.303
2007 Pearson JT, Wahlstrom JL, Dickmann LJ, Kumar S, Halpert JR, Wienkers LC, Foti RS, Rock DA. Differential time-dependent inactivation of P450 3A4 and P450 3A5 by raloxifene: a key role for C239 in quenching reactive intermediates. Chemical Research in Toxicology. 20: 1778-86. PMID 18001057 DOI: 10.1021/Tx700207U  0.33
2007 Zhao Y, Sun L, Muralidhara BK, Kumar S, White MA, Stout CD, Halpert JR. Structural and thermodynamic consequences of 1-(4-chlorophenyl)imidazole binding to cytochrome P450 2B4. Biochemistry. 46: 11559-67. PMID 17887776 DOI: 10.1021/Bi7011614  0.316
2007 Muralidhara BK, Halpert JR. Thermodynamics of ligand binding to P450 2B4 and P450eryF studied by isothermal titration calorimetry. Drug Metabolism Reviews. 39: 539-56. PMID 17786637 DOI: 10.1080/03602530701498182  0.314
2007 Kumar S, Zhao Y, Sun L, Negi SS, Halpert JR, Muralidhara BK. Rational engineering of human cytochrome P450 2B6 for enhanced expression and stability: Importance of a Leu264→Phe substitution Molecular Pharmacology. 72: 1191-1199. PMID 17715394 DOI: 10.1124/Mol.107.039693  0.382
2007 Fernando H, Davydov DR, Chin CC, Halpert JR. Role of subunit interactions in P450 oligomers in the loss of homotropic cooperativity in the cytochrome P450 3A4 mutant L211F/D214E/F304W. Archives of Biochemistry and Biophysics. 460: 129-40. PMID 17274942 DOI: 10.1016/J.Abb.2006.12.025  0.307
2007 Zhao Y, Halpert JR. Structure-function analysis of cytochromes P450 2B. Biochimica Et Biophysica Acta. 1770: 402-12. PMID 16935426 DOI: 10.1016/J.Bbagen.2006.07.006  0.343
2006 Kumar S, Liu H, Halpert JR. Engineering of cytochrome P450 3A4 for enhanced peroxide-mediated substrate oxidation using directed evolution and site-directed mutagenesis. Drug Metabolism and Disposition: the Biological Fate of Chemicals. 34: 1958-65. PMID 16987939 DOI: 10.1124/dmd.106.012054  0.315
2006 Mast N, Murtazina D, Liu H, Graham SE, Bjorkhem I, Halpert JR, Peterson J, Pikuleva IA. Distinct binding of cholesterol and 5β-cholestane-3α,7α, 12α-triol to cytochrome P450 27A1: Evidence from modeling and site-directed mutagenesis studies Biochemistry. 45: 4396-4404. PMID 16584175 DOI: 10.1021/Bi052654W  0.317
2006 Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD. Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction. The Journal of Biological Chemistry. 281: 5973-81. PMID 16373351 DOI: 10.1074/Jbc.M511464200  0.337
2005 Kumar S, Halpert JR. Use of directed evolution of mammalian cytochromes P450 for investigating the molecular basis of enzyme function and generating novel biocatalysts. Biochemical and Biophysical Research Communications. 338: 456-64. PMID 16126165 DOI: 10.1016/J.Bbrc.2005.08.080  0.317
2005 Li W, Liu H, Scott EE, Gräter F, Halpert JR, Luo X, Shen J, Jiang H. Possible pathway(s) of testosterone egress from the active site of cytochrome P450 2B1: a steered molecular dynamics simulation. Drug Metabolism and Disposition: the Biological Fate of Chemicals. 33: 910-9. PMID 15821040 DOI: 10.1124/Dmd.105.004200  0.585
2005 Kumar S, Chen CS, Waxman DJ, Halpert JR. Directed evolution of mammalian cytochrome P450 2B1: mutations outside of the active site enhance the metabolism of several substrates, including the anticancer prodrugs cyclophosphamide and ifosfamide. The Journal of Biological Chemistry. 280: 19569-75. PMID 15774478 DOI: 10.1074/Jbc.M500158200  0.319
2005 Honma W, Li W, Liu H, Scott EE, Halpert JR. Functional role of residues in the helix B' region of cytochrome P450 2B1. Archives of Biochemistry and Biophysics. 435: 157-65. PMID 15680917 DOI: 10.1016/J.Abb.2004.12.014  0.655
2005 Scott EE, Halpert JR. Structures of cytochrome P450 3A4. Trends in Biochemical Sciences. 30: 5-7. PMID 15653318 DOI: 10.1016/J.Tibs.2004.11.004  0.635
2004 Chen CS, Lin JT, Goss KA, He YA, Halpert JR, Waxman DJ. Activation of the anticancer prodrugs cyclophosphamide and ifosfamide: identification of cytochrome P450 2B enzymes and site-specific mutants with improved enzyme kinetics. Molecular Pharmacology. 65: 1278-85. PMID 15102956 DOI: 10.1124/Mol.65.5.1278  0.348
2004 Scott EE, White MA, He YA, Johnson EF, Stout CD, Halpert JR. Structure of mammalian cytochrome P450 2B4 complexed with 4-(4-chlorophenyl)imidazole at 1.9-A resolution: insight into the range of P450 conformations and the coordination of redox partner binding. The Journal of Biological Chemistry. 279: 27294-301. PMID 15100217 DOI: 10.1074/Jbc.M403349200  0.661
2004 Scott EE, Liu H, Qun He Y, Li W, Halpert JR. Mutagenesis and molecular dynamics suggest structural and functional roles for residues in the N-terminal portion of the cytochrome P450 2B1 I helix. Archives of Biochemistry and Biophysics. 423: 266-76. PMID 15001391 DOI: 10.1016/J.Abb.2003.12.035  0.645
2003 Scott EE, He YA, Wester MR, White MA, Chin CC, Halpert JR, Johnson EF, Stout CD. An open conformation of mammalian cytochrome P450 2B4 at 1.6-A resolution. Proceedings of the National Academy of Sciences of the United States of America. 100: 13196-201. PMID 14563924 DOI: 10.1073/Pnas.2133986100  0.631
2003 Kumar S, Scott EE, Liu H, Halpert JR. A rational approach to Re-engineer cytochrome P450 2B1 regioselectivity based on the crystal structure of cytochrome P450 2C5. The Journal of Biological Chemistry. 278: 17178-84. PMID 12609983 DOI: 10.1074/Jbc.M212515200  0.623
2003 Spatzenegger M, Liu H, Wang Q, Debarber A, Koop DR, Halpert JR. Analysis of differential substrate selectivities of CYP2B6 and CYP2E1 by site-directed mutagenesis and molecular modeling. The Journal of Pharmacology and Experimental Therapeutics. 304: 477-87. PMID 12490624 DOI: 10.1124/Jpet.102.043323  0.36
2003 He YA, Roussel F, Halpert JR. Analysis of homotropic and heterotropic cooperativity of diazepam oxidation by CYP3A4 using site-directed mutagenesis and kinetic modeling. Archives of Biochemistry and Biophysics. 409: 92-101. PMID 12464248 DOI: 10.1016/S0003-9861(02)00484-8  0.314
2002 Scott EE, He YQ, Halpert JR. Substrate routes to the buried active site may vary among cytochromes P450: mutagenesis of the F-G region in P450 2B1. Chemical Research in Toxicology. 15: 1407-13. PMID 12437331 DOI: 10.1021/Tx020057U  0.654
2002 Khan KK, He YQ, Correia MA, Halpert JR. Differential oxidation of mifepristone by cytochromes P450 3A4 and 3A5: selective inactivation of P450 3A4. Drug Metabolism and Disposition: the Biological Fate of Chemicals. 30: 985-90. PMID 12167563  0.305
2002 Khan KK, He YA, He YQ, Halpert JR. Site-directed mutagenesis of cytochrome P450eryF: implications for substrate oxidation, cooperativity, and topology of the active site. Chemical Research in Toxicology. 15: 843-53. PMID 12067252  0.307
2002 Khan KK, He YQ, Domanski TL, Halpert JR. Midazolam oxidation by cytochrome P450 3A4 and active-site mutants: an evaluation of multiple binding sites and of the metabolic pathway that leads to enzyme inactivation. Molecular Pharmacology. 61: 495-506. PMID 11854429 DOI: 10.1124/Mol.61.3.495  0.304
2001 Scott EE, Spatzenegger M, Halpert JR. A truncation of 2B subfamily cytochromes P450 yields increased expression levels, increased solubility, and decreased aggregation while retaining function Archives of Biochemistry and Biophysics. 395: 57-68. PMID 11673866 DOI: 10.1006/Abbi.2001.2574  0.606
2001 Domanski TL, He YQ, Scott EE, Wang Q, Halpert JR. The role of cytochrome 2B1 substrate recognition site residues 115, 294, 297, 298, and 362 in the oxidation of steroids and 7-alkoxycoumarins Archives of Biochemistry and Biophysics. 394: 21-28. PMID 11566023 DOI: 10.1006/Abbi.2001.2504  0.639
1998 Wang H, Dick R, Yin H, Licad-Coles E, Kroetz DL, Szklarz G, Harlow G, Halpert JR, Correia MA. Structure-function relationships of human liver cytochromes P450 3A: aflatoxin B1 metabolism as a probe. Biochemistry. 37: 12536-45. PMID 9730826 DOI: 10.1021/Bi980895G  0.348
1998 Harlow GR, Halpert JR. Analysis of human cytochrome P450 3A4 cooperativity: construction and characterization of a site-directed mutant that displays hyperbolic steroid hydroxylation kinetics. Proceedings of the National Academy of Sciences of the United States of America. 95: 6636-41. PMID 9618464 DOI: 10.1073/Pnas.95.12.6636  0.353
1998 He YQ, Harlow GR, Szklarz GD, Halpert JR. Structural determinants of progesterone hydroxylation by cytochrome P450 2B5: the role of nonsubstrate recognition site residues. Archives of Biochemistry and Biophysics. 350: 333-9. PMID 9473309 DOI: 10.1006/Abbi.1997.0516  0.329
1997 Harlow GR, Halpert JR. Alanine-scanning mutagenesis of a putative substrate recognition site in human cytochrome P450 3A4. Role of residues 210 and 211 in flavonoid activation and substrate specificity. The Journal of Biological Chemistry. 272: 5396-402. PMID 9038138 DOI: 10.1074/Jbc.272.9.5396  0.323
1996 Harlow GR, Halpert JR. Mutagenesis study of Asp-290 in cytochrome P450 2B11 using a fusion protein with rat NADPH-cytochrome P450 reductase. Archives of Biochemistry and Biophysics. 326: 85-92. PMID 8579377 DOI: 10.1006/Abbi.1996.0050  0.319
1994 Szklarz GD, Ornstein RL, Halpert JR. Application of 3-Dimensional Homology Modeling of Cytochrome P450 2B1 for Interpretation of Site-Directed Mutagenesis Results. Journal of Biomolecular Structure & Dynamics. 12: 61-78. PMID 22671908 DOI: 10.1080/07391102.1994.10508088  0.326
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