Stephen G. Withers - Publications

Affiliations: 
1982-2000 Chemistry University of British Columbia, Vancouver, Vancouver, BC, Canada 
Area:
enzyme mechanisms, protein structure/function relationships, enzyme inhibitor design/synthesis and protein engineering
Website:
https://www.chem.ubc.ca/stephen-withers

361 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2020 Zhao J, Si Y, Han Z, Li J, Guo W, Fu Y, Tegl G, Hanson J, Chen HM, Kwan DH, Santana AG, Withers SG. Corrigendum: Facile Formation of β-thioGlcNAc Linkages to Thiol-Containing Sugars, Peptides, and Proteins using a Mutant GH20 Hexosaminidase. Angewandte Chemie (International Ed. in English). 59: 8752. PMID 32449594 DOI: 10.1002/anie.202001582  0.92
2019 Tysoe CR, Caner S, Calvert MB, Win-Mason A, Brayer GD, Withers SG. Synthesis of montbretin A analogues yields potent competitive inhibitors of human pancreatic α-amylase. Chemical Science. 10: 11073-11077. PMID 32206255 DOI: 10.1039/c9sc02610j  0.44
2019 Gao Z, Robinson K, Skowronski DM, De Serres G, Withers SG. Quantification of the total neuraminidase content of recent commercially-available influenza vaccines: Introducing a neuraminidase titration reagent. Vaccine. PMID 31818533 DOI: 10.1016/j.vaccine.2019.11.041  0.48
2019 Rahfeld P, Wardman JF, Mehr K, Huff D, Morgan-Lang C, Chen HM, Hallam SJ, Withers SG. Prospecting for α-N-acetylgalactosaminidases yields a new class of GH31 O-glycanase. The Journal of Biological Chemistry. PMID 31530641 DOI: 10.1074/jbc.RA119.010628  0.44
2019 Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG. An enzymatic pathway in the human gut microbiome that converts A to universal O type blood. Nature Microbiology. PMID 31182795 DOI: 10.1038/s41564-019-0469-7  0.32
2019 Armstrong Z, Liu F, Kheirandish S, Chen HM, Mewis K, Duo T, Morgan-Lang C, Hallam SJ, Withers SG. High-Throughput Recovery and Characterization of Metagenome-Derived Glycoside Hydrolase-Containing Clones as a Resource for Biocatalyst Development. Msystems. 4. PMID 31164449 DOI: 10.1128/mSystems.00082-19  0.44
2019 Janesch B, Baumann L, Mark A, Thompson N, Rahmani S, Sim L, Withers SG, Wakarchuk WW. Directed evolution of bacterial polysialyltransferases. Glycobiology. PMID 30976781 DOI: 10.1093/glycob/cwz021  0.68
2019 Gao Z, Ovchinnikova OG, Huang BS, Liu F, Williams DE, Andersen R, Lowary TL, Whitfield C, Withers SG. A High-Throughput "FP-Tag" Assay for the Identification of Glycosyltransferase Inhibitors. Journal of the American Chemical Society. PMID 30698425 DOI: 10.1021/jacs.8b10940  0.48
2018 Tegl G, Hanson J, Chen HM, Kwan DH, Santana AG, Withers SG. Facile Formation of β-thioGlcNAc Linkages to Thiol-Containing Sugars, Peptides, and Proteins using a Mutant GH20 Hexosaminidase. Angewandte Chemie (International Ed. in English). PMID 30549167 DOI: 10.1002/anie.201809928  0.92
2018 Gao Z, Thompson AJ, Paulson JC, Withers SG. Proximity Ligation-Based Fluorogenic Imaging Agents for Neuraminidases. Angewandte Chemie (International Ed. in English). PMID 30216626 DOI: 10.1002/anie.201808196  0.48
2018 Blaukopf M, Worrall L, Kosma P, Strynadka NCJ, Withers SG. Insights into Heptosyltransferase I Catalysis and Inhibition through the Structure of Its Ternary Complex. Structure (London, England : 1993). PMID 30122450 DOI: 10.1016/j.str.2018.07.001  0.64
2018 Chen HM, Withers SG. Synthesis of azido-deoxy and amino-deoxy glycosides and glycosyl fluorides for screening of glycosidase libraries and assembly of substituted glycosides. Carbohydrate Research. 467: 33-44. PMID 30075363 DOI: 10.1016/j.carres.2018.07.007  0.44
2018 Armstrong Z, Mewis K, Liu F, Morgan-Lang C, Scofield M, Durno E, Chen HM, Mehr K, Withers SG, Hallam SJ. Metagenomics reveals functional synergy and novel polysaccharide utilization loci in the Castor canadensis fecal microbiome. The Isme Journal. PMID 30013164 DOI: 10.1038/s41396-018-0215-9  0.44
2018 Koetzler MP, Robinson K, Chen HM, Okon M, McIntosh LP, Withers SG. Modulating the nucleophile of a glycoside hydrolase through site-specific incorporation of fluoroglutamic acids. Journal of the American Chemical Society. PMID 29894173 DOI: 10.1021/jacs.8b04235  0.44
2018 Smirnova I, Kasho V, Jiang X, Chen HM, Withers SG, Kaback HR. Oversized galactosides as a probe for conformational dynamics in LacY. Proceedings of the National Academy of Sciences of the United States of America. PMID 29602806 DOI: 10.1073/pnas.1800706115  0.44
2018 Macdonald SS, Patel A, Larmour VLC, Morgan-Lang C, Hallam SJ, Mark BL, Withers SG. Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library. The Journal of Biological Chemistry. PMID 29317495 DOI: 10.1074/jbc.RA117.000948  0.52
2017 Duo T, Robinson K, Greig IR, Chen HM, Patrick BO, Withers SG. REMARKABLE REACTIVITY DIFFERENCES BETWEEN GLUCOSIDES WITH IDENTICAL LEAVING GROUPS. Journal of the American Chemical Society. PMID 29035043 DOI: 10.1021/jacs.7b09645  0.44
2017 Lizak C, Worrall LJ, Baumann L, Pfleiderer MM, Volkers G, Sun T, Sim L, Wakarchuk W, Withers SG, Strynadka NCJ. X-ray crystallographic structure of a bacterial polysialyltransferase provides insight into the biosynthesis of capsular polysialic acid. Scientific Reports. 7: 5842. PMID 28724897 DOI: 10.1038/s41598-017-05627-z  0.64
2017 Kötzler MP, McIntosh LP, Withers SG. Refolding the unfoldable: A systematic approach for renaturation of Bacillus circulans xylanase. Protein Science : a Publication of the Protein Society. PMID 28466501 DOI: 10.1002/pro.3181  0.4
2017 Jongkees SA, Caner S, Tysoe C, Brayer GD, Withers SG, Suga H. Rapid Discovery of Potent and Selective Glycosidase-Inhibiting De Novo Peptides. Cell Chemical Biology. PMID 28262556 DOI: 10.1016/j.chembiol.2017.02.001  0.44
2017 Kuatsjah E, Chen HM, Withers SG, Eltis LD. Characterization of an extradiol dioxygenase involved in the catabolism of lignin-derived biphenyl. Febs Letters. PMID 28247503 DOI: 10.1002/1873-3468.12611  0.44
2017 Gao Z, Niikura M, Withers SG. Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration. Angewandte Chemie (International Ed. in English). PMID 28191709 DOI: 10.1002/anie.201610544  0.48
2016 Santana AG, Tysoe C, Hu G, Kronstad J, Goddard-Borger E, Withers S. Fungal glycolipid hydrolase inhibitors and their effect on Cryptococcus neoformans. Chembiochem : a European Journal of Chemical Biology. PMID 27905163 DOI: 10.1002/cbic.201600538  0.4
2016 Zhang X, Caner S, Kwan E, Li C, Brayer GD, Withers SG. Evaluation of the significance of starch surface binding sites on human pancreatic α-amylase. Biochemistry. PMID 27756128 DOI: 10.1021/acs.biochem.6b00992  0.44
2016 Goddard-Borger ED, Tysoe C, Withers SG. Glycosynthase mediated synthesis of psychosine. Carbohydrate Research. 435: 97-99. PMID 27721144 DOI: 10.1016/j.carres.2016.09.013  0.52
2016 Zhang XF, Yang GY, Zhang Y, Xie Y, Withers SG, Feng Y. A general and efficient strategy for generating the stable enzymes. Scientific Reports. 6: 33797. PMID 27667190 DOI: 10.1038/srep33797  1
2016 Ma F, Fischer M, Han Y, Withers SG, Feng Y, Yang GY. Substrate Engineering Enabling Fluorescence Droplet Entrapment for IVC-FACS Based Ultrahigh-Throughput Screening. Analytical Chemistry. PMID 27494801 DOI: 10.1021/acs.analchem.6b01712  1
2016 Arns S, Tan J, Sun S, Galey A, Zisman N, Ross F, Udechukwu J, Dercho S, Gusti V, Paquette J, Wennekes T, Webb M, Bourque E, Withers SG, Liggins R. Corrigendum to "Assessing the oral bioavailability of difluorosialic acid prodrugs, potent viral neuraminidase inhibitors, using a snapshot PK screening assay" [Bioorg. Med. Chem. Lett. 25 (2015) 2505-2509]. Bioorganic & Medicinal Chemistry Letters. PMID 27381085 DOI: 10.1016/j.bmcl.2016.06.068  0.4
2016 Santana AG, Vadlamani G, Mark BL, Withers SG. N-Acetyl glycals are tight-binding and environmentally insensitive inhibitors of hexosaminidases. Chemical Communications (Cambridge, England). 52: 7943-6. PMID 27253678 DOI: 10.1039/c6cc02520j  0.4
2016 Zoidl M, Gonzalez Santana A, Torvisco A, Tysoe C, Siriwardena A, Withers SG, Wrodnigg TM. The Staudinger/aza-Wittig/Grignard reaction as key step for the concise synthesis of 1-C-Alkyl-iminoalditol glycomimetics. Carbohydrate Research. PMID 27160850 DOI: 10.1016/j.carres.2016.04.006  1
2016 Tysoe C, Williams LK, Keyzers R, Nguyen NT, Tarling C, Wicki J, Goddard-Borger ED, Aguda AH, Perry S, Foster LJ, Andersen RJ, Brayer GD, Withers SG. Potent Human α-Amylase Inhibition by the β-Defensin-like Protein Helianthamide. Acs Central Science. 2: 154-161. PMID 27066537 DOI: 10.1021/acscentsci.5b00399  0.52
2016 Thonhofer M, Weber P, Gonzalez Santana A, Tysoe C, Fischer R, Pabst BM, Paschke E, Schalli M, Stütz AE, Tschernutter M, Windischhofer W, Withers SG. Synthesis of C-5a-substituted derivatives of 4-epi-isofagomine: notable β-galactosidase inhibitors and activity promotors of GM1-gangliosidosis related human lysosomal β-galactosidase mutant R201C. Carbohydrate Research. PMID 27063389 DOI: 10.1016/j.carres.2016.03.020  1
2016 Caner S, Zhang X, Jiang J, Chen HM, Nguyen NT, Overkleeft H, Brayer GD, Withers SG. Glucosyl Epi-cyclophellitol Allows Mechanism- Based Inactivation and Structural Analysis of Human Pancreatic Alpha-Amylase. Febs Letters. PMID 27000970 DOI: 10.1002/1873-3468.12143  1
2016 Morgan JL, McNamara JT, Fischer M, Rich J, Chen HM, Withers SG, Zimmer J. Observing cellulose biosynthesis and membrane translocation in crystallo. Nature. PMID 26958837 DOI: 10.1038/nature16966  0.44
2016 Thonhofer M, Weber P, Santana AG, Fischer R, Pabst BM, Paschke E, Schalli M, Stütz AE, Tschernutter M, Windischhofer W, Withers SG. Synthesis of C-5a-chain extended derivatives of 4-epi-isofagomine: Powerful β-galactosidase inhibitors and low concentration activators of GM1-gangliosidosis-related human lysosomal β-galactosidase. Bioorganic & Medicinal Chemistry Letters. PMID 26838810 DOI: 10.1016/j.bmcl.2016.01.059  1
2016 Kwan DH, Jin Y, Jiang J, Chen HM, Kötzler MP, Overkleeft HS, Davies G, Withers SG. Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-β-glucosidases. Febs Letters. PMID 26790390 DOI: 10.1002/1873-3468.12059  1
2016 Chen HM, Armstrong Z, Hallam SJ, Withers SG. Synthesis and evaluation of a series of 6-chloro-4-methylumbelliferyl glycosides as fluorogenic reagents for screening metagenomic libraries for glycosidase activity. Carbohydrate Research. 421: 33-39. PMID 26774876 DOI: 10.1016/j.carres.2015.12.010  1
2015 Thonhofer M, Gonzalez Santana A, Fischer R, Torvisco Gomez A, Saf R, Schalli M, Stütz AE, Withers SG. 5-Fluoro derivatives of 4-epi-isofagomine as d-galactosidase inhibitors and potential pharmacological chaperones for GM1-gangliosidosis as well as Fabry's disease. Carbohydrate Research. 420: 6-12. PMID 26717544 DOI: 10.1016/j.carres.2015.10.009  1
2015 Mehr K, Withers SG. Mechanisms of the Sialidase and Trans-sialidase Activities of Bacterial Sialyltransferases from Glycosyltransferase Family 80 (GT80). Glycobiology. PMID 26582604 DOI: 10.1093/glycob/cwv105  1
2015 Yuen VG, Coleman J, Withers SG, Andersen RJ, Brayer GD, Mustafa S, McNeill JH. Glucose lowering effect of montbretin A in Zucker Diabetic Fatty rats. Molecular and Cellular Biochemistry. PMID 26547551 DOI: 10.1007/s11010-015-2599-4  0.44
2015 Kötzler MP, Withers SG. Proteolytic cleavage driven by glycosylation. The Journal of Biological Chemistry. PMID 26515062 DOI: 10.1074/jbc.C115.698696  1
2015 Tai SS, Agafitei O, Gao Z, Liggins R, Petric M, Withers SG, Niikura M. Difluorosialic acids, potent novel influenza virus neuraminidase inhibitors, induce fewer drug resistance-associated neuraminidase mutations than does oseltamivir. Virus Research. 210: 126-132. PMID 26247418 DOI: 10.1016/j.virusres.2015.07.015  1
2015 Williams LK, Zhang X, Caner S, Tysoe C, Nguyen NT, Wicki J, Williams DE, Coleman J, McNeill JH, Yuen V, Andersen RJ, Withers SG, Brayer GD. The amylase inhibitor montbretin A reveals a new glycosidase inhibition motif. Nature Chemical Biology. 11: 691-6. PMID 26214255 DOI: 10.1038/nchembio.1865  1
2015 Volkers G, Worrall LJ, Kwan DH, Yu CC, Baumann L, Lameignere E, Wasney GA, Scott NE, Wakarchuk W, Foster LJ, Withers SG, Strynadka NC. Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation. Nature Structural & Molecular Biology. 22: 627-35. PMID 26192331 DOI: 10.1038/nsmb.3060  0.92
2015 Arns S, Tan J, Sun S, Galey A, Zisman N, Ross F, Udechukwu J, Dercho S, Gusti V, Paquette J, Webb M, Bourque E, Withers SG, Liggins R. Assessing the oral bioavailability of difluorosialic acid prodrugs, potent viral neuraminidase inhibitors, using a snapshot PK screening assay. Bioorganic & Medicinal Chemistry Letters. 25: 2505-9. PMID 25980910 DOI: 10.1016/j.bmcl.2015.04.059  1
2015 Kwan DH, Ernst S, Kötzler MP, Withers SG. Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes. Glycobiology. 25: 806-11. PMID 25964111 DOI: 10.1093/glycob/cwv031  1
2015 Kwan DH, Constantinescu I, Chapanian R, Higgins MA, Kötzler MP, Samain E, Boraston AB, Kizhakkedathu JN, Withers SG. Toward Efficient Enzymes for the Generation of Universal Blood through Structure-Guided Directed Evolution. Journal of the American Chemical Society. 137: 5695-705. PMID 25870881 DOI: 10.1021/ja5116088  1
2015 Yang GY, Li C, Fischer M, Cairo CW, Feng Y, Withers SG. A FRET probe for cell-based imaging of ganglioside-processing enzyme activity and high-throughput screening. Angewandte Chemie (International Ed. in English). 54: 5389-93. PMID 25757223 DOI: 10.1002/anie.201411747  1
2015 Han YB, Wu L, Rich JR, Huang FT, Withers SG, Feng Y, Yang GY. Comprehensive characterization of sphingolipid ceramide N-deacylase for the synthesis and fatty acid remodeling of glycosphingolipids. Applied Microbiology and Biotechnology. 99: 6715-26. PMID 25683662 DOI: 10.1007/s00253-015-6421-8  1
2015 Sobhanifar S, Worrall LJ, Gruninger RJ, Wasney GA, Blaukopf M, Baumann L, Lameignere E, Solomonson M, Brown ED, Withers SG, Strynadka NC. Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase. Proceedings of the National Academy of Sciences of the United States of America. 112: E576-85. PMID 25624472 DOI: 10.1073/pnas.1418084112  1
2015 Weck S, Robinson K, Smith MR, Withers SG. Understanding viral neuraminidase inhibition by substituted difluorosialic acids. Chemical Communications (Cambridge, England). 51: 2933-5. PMID 25587931 DOI: 10.1039/c4cc08256g  1
2015 Macdonald SS, Blaukopf M, Withers SG. N-acetylglucosaminidases from CAZy family GH3 are really glycoside phosphorylases, thereby explaining their use of histidine as an acid/base catalyst in place of glutamic acid. The Journal of Biological Chemistry. 290: 4887-95. PMID 25533455 DOI: 10.1074/jbc.M114.621110  1
2015 Volkers G, Worrall LJ, Kwan DH, Yu CC, Baumann L, Lameignere E, Wasney GA, Scott NE, Wakarchuk W, Foster LJ, Withers SG, Strynadka NCJ. Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation Nature Structural and Molecular Biology. DOI: 10.1038/nsmb.3060  1
2015 Yu CC, Withers SG. Recent developments in enzymatic synthesis of modified sialic acid derivatives Advanced Synthesis and Catalysis. 357: 1633-1654. DOI: 10.1002/adsc.201500349  1
2015 Gesell A, Blaukopf M, Madilao L, Yuen MMS, Withers SG, Mattsson J, Russell JH, Bohlmann J. The gymnosperm cytochrome P450 CYP750B1 catalyzes stereospecific monoterpene hydroxylation of (+)-sabinene in thujone biosynthesis in western redcedar Plant Physiology. 168: 94-106. DOI: /10.1104/pp.15.00315  1
2014 Chapanian R, Kwan DH, Constantinescu I, Shaikh FA, Rossi NA, Withers SG, Kizhakkedathu JN. Enhancement of biological reactions on cell surfaces via macromolecular crowding. Nature Communications. 5: 4683. PMID 25140641 DOI: 10.1038/ncomms5683  1
2014 Kwan DH, Withers SG. Periplasmic de-acylase helps bacteria don their biofilm coat. Proceedings of the National Academy of Sciences of the United States of America. 111: 10904-5. PMID 25006258 DOI: 10.1073/pnas.1410789111  1
2014 Duo T, Goddard-Borger ED, Withers SG. Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining β-glycosidases. Chemical Communications (Cambridge, England). 50: 9379-82. PMID 25004867 DOI: 10.1039/c4cc03299c  1
2014 Zoidl M, Müller B, Torvisco A, Tysoe C, Benazza M, Siriwardena A, Withers SG, Wrodnigg TM. Concise synthesis of C-1-cyano-iminosugars via a new Staudinger/aza Wittig/Strecker multicomponent reaction strategy. Bioorganic & Medicinal Chemistry Letters. 24: 2777-80. PMID 24803362 DOI: 10.1016/j.bmcl.2014.03.069  1
2014 Syson K, Stevenson CE, Rashid AM, Saalbach G, Tang M, Tuukkanen A, Svergun DI, Withers SG, Lawson DM, Bornemann S. Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds α-maltose 1-phosphate and forms a maltosyl-enzyme intermediate. Biochemistry. 53: 2494-504. PMID 24689960 DOI: 10.1021/bi500183c  1
2014 Rempel BP, Withers SG. Phosphodiesters serve as potentially tunable aglycones for fluoro sugar inactivators of retaining β-glycosidases. Organic & Biomolecular Chemistry. 12: 2592-5. PMID 24618676 DOI: 10.1039/c4ob00235k  1
2014 Buchini S, Gallat FX, Greig IR, Kim JH, Wakatsuki S, Chavas LM, Withers SG. Tuning mechanism-based inactivators of neuraminidases: mechanistic and structural insights. Angewandte Chemie (International Ed. in English). 53: 3382-6. PMID 24591206 DOI: 10.1002/anie.201309675  1
2014 Jongkees SA, Yoo H, Withers SG. Mechanistic investigations of unsaturated glucuronyl hydrolase from Clostridium perfringens. The Journal of Biological Chemistry. 289: 11385-95. PMID 24573682 DOI: 10.1074/jbc.M113.545293  1
2014 Tysoe C, Withers SG. Fluorinated mechanism-based inhibitors: common themes and recent developments. Current Topics in Medicinal Chemistry. 14: 865-74. PMID 24484426 DOI: 10.2174/1568026614666140202204602  1
2014 Knott BC, Haddad Momeni M, Crowley MF, Mackenzie LF, Götz AW, Sandgren M, Withers SG, Ståhlberg J, Beckham GT. The mechanism of cellulose hydrolysis by a two-step, retaining cellobiohydrolase elucidated by structural and transition path sampling studies. Journal of the American Chemical Society. 136: 321-9. PMID 24341799 DOI: 10.1021/ja410291u  1
2014 Jongkees SA, Yoo H, Withers SG. Mechanistic insights from substrate preference in unsaturated glucuronyl hydrolase. Chembiochem : a European Journal of Chemical Biology. 15: 124-34. PMID 24227702 DOI: 10.1002/cbic.201300547  1
2014 Yu CC, Hill T, Kwan DH, Chen HM, Lin CC, Wakarchuk W, Withers SG. A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis. Analytical Biochemistry. 444: 67-74. PMID 24121013 DOI: 10.1016/j.ab.2013.09.030  1
2014 Jongkees SA, Withers SG. Unusual enzymatic glycoside cleavage mechanisms. Accounts of Chemical Research. 47: 226-35. PMID 23957528 DOI: 10.1021/ar4001313  1
2013 Yu CC, Huang LD, Kwan DH, Wakarchuk WW, Withers SG, Lin CC. A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis. Chemical Communications (Cambridge, England). 49: 10166-8. PMID 24051967 DOI: 10.1039/c3cc45147j  1
2013 Bie H, Yin J, He X, Kermode AR, Goddard-Borger ED, Withers SG, James MN. Insights into mucopolysaccharidosis I from the structure and action of α-L-iduronidase. Nature Chemical Biology. 9: 739-45. PMID 24036510 DOI: 10.1038/nchembio.1357  1
2013 Mewis K, Armstrong Z, Song YC, Baldwin SA, Withers SG, Hallam SJ. Biomining active cellulases from a mining bioremediation system. Journal of Biotechnology. 167: 462-71. PMID 23906845 DOI: 10.1016/j.jbiotec.2013.07.015  1
2013 Shaikh FA, Lammerts van Bueren A, Davies GJ, Withers SG. Identifying the catalytic acid/base in GH29 α-L-fucosidase subfamilies. Biochemistry. 52: 5857-64. PMID 23883131 DOI: 10.1021/bi400183q  1
2013 Armstrong Z, Withers SG. Synthesis of glycans and glycopolymers through engineered enzymes. Biopolymers. 99: 666-74. PMID 23821499 DOI: 10.1002/bip.22335  1
2013 Caner S, Nguyen N, Aguda A, Zhang R, Pan YT, Withers SG, Brayer GD. The structure of the Mycobacterium smegmatis trehalose synthase reveals an unusual active site configuration and acarbose-binding mode. Glycobiology. 23: 1075-83. PMID 23735230 DOI: 10.1093/glycob/cwt044  1
2013 Ratananikom K, Choengpanya K, Tongtubtim N, Charoenrat T, Withers SG, Kongsaeree PT. Mutational analysis in the glycone binding pocket of Dalbergia cochinchinensis β-glucosidase to increase catalytic efficiency toward mannosides. Carbohydrate Research. 373: 35-41. PMID 23583451 DOI: 10.1016/j.carres.2012.10.018  1
2013 Ludwiczek ML, D'Angelo I, Yalloway GN, Brockerman JA, Okon M, Nielsen JE, Strynadka NC, Withers SG, McIntosh LP. Strategies for modulating the pH-dependent activity of a family 11 glycoside hydrolase. Biochemistry. 52: 3138-56. PMID 23578322 DOI: 10.1021/bi400034m  1
2013 Kim JH, Resende R, Wennekes T, Chen HM, Bance N, Buchini S, Watts AG, Pilling P, Streltsov VA, Petric M, Liggins R, Barrett S, McKimm-Breschkin JL, Niikura M, Withers SG. Mechanism-based covalent neuraminidase inhibitors with broad-spectrum influenza antiviral activity. Science (New York, N.Y.). 340: 71-5. PMID 23429702 DOI: 10.1126/science.1232552  1
2013 Thompson J, Pikis A, Rich J, Hall BG, Withers SG. α-Galacturonidase(s): a new class of Family 4 glycoside hydrolases with strict specificity and a unique CHEV active site motif. Febs Letters. 587: 799-803. PMID 23416295 DOI: 10.1016/j.febslet.2013.02.004  1
2013 Rakic B, Rao FV, Freimann K, Wakarchuk W, Strynadka NC, Withers SG. Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: porcine ST3Gal-I. Glycobiology. 23: 536-45. PMID 23300007 DOI: 10.1093/glycob/cwt001  1
2013 Stocker BL, Jongkees SA, Win-Mason AL, Dangerfield EM, Withers SG, Timmer MS. The 'mirror-image' postulate as a guide to the selection and evaluation of pyrrolidines as α-l-fucosidase inhibitors. Carbohydrate Research. 367: 29-32. PMID 23295913 DOI: 10.1016/j.carres.2012.11.011  1
2013 Chan PH, Cheung AH, Okon M, Chen HM, Withers SG, McIntosh LP. Investigating the structural dynamics of α-1,4-galactosyltransferase C from Neisseria meningitidis by nuclear magnetic resonance spectroscopy. Biochemistry. 52: 320-32. PMID 23259770 DOI: 10.1021/bi301317d  1
2013 Shim JH, Withers SG. Improvement of the expression level of β-glucosidase from Agrobacterium sp. in Escherichia coli by rare codon optimization Food Science and Biotechnology. 22: 269-273. DOI: 10.1007/s10068-013-0036-6  1
2012 Vocadlo DJ, Withers SG. How to make a difference: mechanisms of protein and nucleic acid modifying enzymes. Current Opinion in Chemical Biology. 16: 461-4. PMID 23168221 DOI: 10.1016/j.cbpa.2012.10.033  1
2012 Withers SG, Davies GJ. Glycobiology: The case of the missing base. Nature Chemical Biology. 8: 952-3. PMID 23103941 DOI: 10.1038/nchembio.1117  1
2012 Williams LK, Li C, Withers SG, Brayer GD. Order and disorder: differential structural impacts of myricetin and ethyl caffeate on human amylase, an antidiabetic target. Journal of Medicinal Chemistry. 55: 10177-86. PMID 23050660 DOI: 10.1021/jm301273u  1
2012 Yip VL, Withers SG. Identification of Tyr241 as a key catalytic base in the family 4 glycoside hydrolase BglT from Thermotoga maritima. Biochemistry. 51: 8464-74. PMID 23025815 DOI: 10.1021/bi301021u  1
2012 Chan PH, Weissbach S, Okon M, Withers SG, McIntosh LP. Nuclear magnetic resonance spectral assignments of α-1,4-galactosyltransferase LgtC from Neisseria meningitidis: substrate binding and multiple conformational states. Biochemistry. 51: 8278-92. PMID 22992161 DOI: 10.1021/bi3010279  1
2012 Shim JH, Chen HM, Rich JR, Goddard-Borger ED, Withers SG. Directed evolution of a β-glycosidase from Agrobacterium sp. to enhance its glycosynthase activity toward C3-modified donor sugars. Protein Engineering, Design & Selection : Peds. 25: 465-72. PMID 22893693 DOI: 10.1093/protein/gzs045  1
2012 Rich JR, Withers SG. A chemoenzymatic total synthesis of the neurogenic starfish ganglioside LLG-3 using an engineered and evolved synthase. Angewandte Chemie (International Ed. in English). 51: 8640-3. PMID 22821741 DOI: 10.1002/anie.201204578  1
2012 Reitinger S, Petriv OI, Mehr K, Hansen CL, Withers SG. Purification and quantitation of bacteriophage M13 using desalting spin columns and digital PCR. Journal of Virological Methods. 185: 171-4. PMID 22766184 DOI: 10.1016/j.jviromet.2012.06.021  1
2012 Goddard-Borger ED, Withers SG. Natural product biosynthesis: Tackling tunicamycin. Nature Chemistry. 4: 520-1. PMID 22717432 DOI: 10.1038/nchem.1391  1
2012 Pengthaisong S, Chen CF, Withers SG, Kuaprasert B, Ketudat Cairns JR. Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition. Carbohydrate Research. 352: 51-9. PMID 22418094 DOI: 10.1016/j.carres.2012.02.013  1
2012 Michikawa M, Ichinose H, Momma M, Biely P, Jongkees S, Yoshida M, Kotake T, Tsumuraya Y, Withers SG, Fujimoto Z, Kaneko S. Structural and biochemical characterization of glycoside hydrolase family 79 β-glucuronidase from Acidobacterium capsulatum. The Journal of Biological Chemistry. 287: 14069-77. PMID 22367201 DOI: 10.1074/jbc.M112.346288  1
2012 Goddard-Borger ED, Tropak MB, Yonekawa S, Tysoe C, Mahuran DJ, Withers SG. Rapid assembly of a library of lipophilic iminosugars via the thiol-ene reaction yields promising pharmacological chaperones for the treatment of Gaucher disease. Journal of Medicinal Chemistry. 55: 2737-45. PMID 22360565 DOI: 10.1021/jm201633y  1
2012 Goddard-Borger ED, Sakaguchi K, Reitinger S, Watanabe N, Ito M, Withers SG. Mechanistic insights into the 1,3-xylanases: useful enzymes for manipulation of algal biomass. Journal of the American Chemical Society. 134: 3895-902. PMID 22296113 DOI: 10.1021/ja211836t  1
2012 Pengthaisong S, Withers SG, Kuaprasert B, Svasti J, Cairns JR. The role of the oligosaccharide binding cleft of rice BGlu1 in hydrolysis of cellooligosaccharides and in their synthesis by rice BGlu1 glycosynthase. Protein Science : a Publication of the Protein Society. 21: 362-72. PMID 22238157 DOI: 10.1002/pro.2021  1
2011 Jongkees SA, Withers SG. Glycoside cleavage by a new mechanism in unsaturated glucuronyl hydrolases. Journal of the American Chemical Society. 133: 19334-7. PMID 22047074 DOI: 10.1021/ja209067v  1
2011 Win-Mason AL, Jongkees SA, Withers SG, Tyler PC, Timmer MS, Stocker BL. Stereoselective total synthesis of aminoiminohexitols via carbamate annulation. The Journal of Organic Chemistry. 76: 9611-21. PMID 22022891 DOI: 10.1021/jo201151b  1
2011 Fröhlich RF, Fantur K, Furneaux RH, Paschke E, Stütz AE, Wicki J, Withers SG, Wrodnigg TM. A fluorescent probe for GM1 gangliosidosis related β-galactosidase: N-(dansylamino)hexylaminocarbonylpentyl-1,5-dideoxy-1,5-imino-D-galactitol. Bioorganic & Medicinal Chemistry Letters. 21: 6872-5. PMID 21974950 DOI: 10.1016/j.bmcl.2011.09.012  1
2011 Rempel BP, Tropak MB, Mahuran DJ, Withers SG. Tailoring the specificity and reactivity of a mechanism-based inactivator of glucocerebrosidase for potential therapeutic applications. Angewandte Chemie (International Ed. in English). 50: 10381-3. PMID 21915968 DOI: 10.1002/anie.201103924  1
2011 Lee SS, Greig IR, Vocadlo DJ, McCarter JD, Patrick BO, Withers SG. Structural, mechanistic, and computational analysis of the effects of anomeric fluorines on anomeric fluoride departure in 5-fluoroxylosyl fluorides. Journal of the American Chemical Society. 133: 15826-9. PMID 21910446 DOI: 10.1021/ja204829r  1
2011 Lin LY, Rakic B, Chiu CP, Lameignere E, Wakarchuk WW, Withers SG, Strynadka NC. Structure and mechanism of the lipooligosaccharide sialyltransferase from Neisseria meningitidis. The Journal of Biological Chemistry. 286: 37237-48. PMID 21880735 DOI: 10.1074/jbc.M111.249920  1
2011 Rich JR, Cunningham AM, Gilbert M, Withers SG. Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase. Chemical Communications (Cambridge, England). 47: 10806-8. PMID 21879043 DOI: 10.1039/c1cc13885e  1
2011 Zhang R, Pan YT, He S, Lam M, Brayer GD, Elbein AD, Withers SG. Mechanistic analysis of trehalose synthase from Mycobacterium smegmatis. The Journal of Biological Chemistry. 286: 35601-9. PMID 21840994 DOI: 10.1074/jbc.M111.280362  1
2011 Lee HJ, Lairson LL, Rich JR, Lameignere E, Wakarchuk WW, Withers SG, Strynadka NC. Structural and kinetic analysis of substrate binding to the sialyltransferase Cst-II from Campylobacter jejuni. The Journal of Biological Chemistry. 286: 35922-32. PMID 21832050 DOI: 10.1074/jbc.M111.261172  1
2011 Hill T, Tropak MB, Mahuran D, Withers SG. Synthesis, kinetic evaluation and cell-based analysis of C-alkylated isofagomines as chaperones of β-glucocerebrosidase. Chembiochem : a European Journal of Chemical Biology. 12: 2151-4. PMID 21751327 DOI: 10.1002/cbic.201100332  1
2011 Goddard-Borger ED, Fiege B, Kwan EM, Withers SG. Glycosynthase-mediated assembly of xylanase substrates and inhibitors. Chembiochem : a European Journal of Chemical Biology. 12: 1703-11. PMID 21710681 DOI: 10.1002/cbic.201100229  1
2011 Torpenholt S, Le Nours J, Christensen U, Jahn M, Withers S, Østergaard PR, Borchert TV, Poulsen JC, Leggio LL. Activity of three β-1,4-galactanases on small chromogenic substrates Carbohydrate Research. 346: 2028-2033. PMID 21696710 DOI: 10.1016/j.carres.2011.05.017  1
2011 Fröhlich RF, Furneaux RH, Mahuran DJ, Saf R, Stütz AE, Tropak MB, Wicki J, Withers SG, Wrodnigg TM. 1-Deoxy-D-galactonojirimycins with dansyl capped N-substituents as β-galactosidase inhibitors and potential probes for GM1 gangliosidosis affected cell lines. Carbohydrate Research. 346: 1592-8. PMID 21645885 DOI: 10.1016/j.carres.2011.05.010  1
2011 Kwan DH, Chen HM, Ratananikom K, Hancock SM, Watanabe Y, Kongsaeree PT, Samuels AL, Withers SG. Self-immobilizing fluorogenic imaging agents of enzyme activity. Angewandte Chemie (International Ed. in English). 50: 300-3. PMID 21184404 DOI: 10.1002/anie.201005705  1
2011 Kwan DH, Withers SG. Toward efficient enzymatic glycan synthesis: Directed evolution and enzyme engineering Journal of Carbohydrate Chemistry. 30: 181-205. DOI: 10.1080/07328303.2011.604457  1
2011 Aharoni A, Withers SG. Screening Methodologies for Glycosidic Bond Formation Protein Engineering Handbook, Volume 1 & Volume 2. 2: 605-620. DOI: 10.1002/9783527634026.ch25  1
2010 Goddard-Borger ED, Wennekes T, Withers SG. Molecular probes: Getting lucky in the lysosome. Nature Chemical Biology. 6: 881-3. PMID 21079598 DOI: 10.1038/nchembio.470  1
2010 Chen HM, Withers SG. Syntheses of p-nitrophenyl 3- and 4-thio-β-D-glycopyranosides. Carbohydrate Research. 345: 2596-604. PMID 21044780 DOI: 10.1016/j.carres.2010.10.001  1
2010 Kim YW, Zhang R, Chen H, Withers SG. O-glycoligases, a new category of glycoside bond-forming mutant glycosidases, catalyse facile syntheses of isoprimeverosides. Chemical Communications (Cambridge, England). 46: 8725-7. PMID 21031187 DOI: 10.1039/c0cc03168b  1
2010 Kim EJ, Love DC, Darout E, Abdo M, Rempel B, Withers SG, Rablen PR, Hanover JA, Knapp S. OGA inhibition by GlcNAc-selenazoline. Bioorganic & Medicinal Chemistry. 18: 7058-64. PMID 20822912 DOI: 10.1016/j.bmc.2010.08.010  1
2010 Schitter G, Steiner AJ, Pototschnig G, Scheucher E, Thonhofer M, Tarling CA, Withers SG, Fantur K, Paschke E, Mahuran DJ, Rigat BA, Tropak MB, Illaszewicz C, Saf R, Stütz AE, et al. Fluorous iminoalditols: a new family of glycosidase inhibitors and pharmacological chaperones. Chembiochem : a European Journal of Chemical Biology. 11: 2026-33. PMID 20715263 DOI: 10.1002/cbic.201000192  1
2010 Yang G, Rich JR, Gilbert M, Wakarchuk WW, Feng Y, Withers SG. Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases. Journal of the American Chemical Society. 132: 10570-7. PMID 20662530 DOI: 10.1021/ja104167y  1
2010 Pototschnig G, De Csáky CM, Burke JR, Schitter G, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. Synthesis and biological evaluation of novel biotin-iminoalditol conjugates. Bioorganic & Medicinal Chemistry Letters. 20: 4077-9. PMID 20610152 DOI: 10.1016/j.bmcl.2010.05.084  1
2010 Morley TJ, Withers SG. Chemoenzymatic synthesis and enzymatic analysis of 8-modified cytidine monophosphate-sialic acid and sialyl lactose derivatives. Journal of the American Chemical Society. 132: 9430-7. PMID 20557044 DOI: 10.1021/ja102644a  1
2010 Phenix CP, Rempel BP, Colobong K, Doudet DJ, Adam MJ, Clarke LA, Withers SG. Imaging of enzyme replacement therapy using PET. Proceedings of the National Academy of Sciences of the United States of America. 107: 10842-7. PMID 20534487 DOI: 10.1073/pnas.1003247107  1
2010 Schitter G, Scheucher E, Steiner AJ, Stütz AE, Thonhofer M, Tarling CA, Withers SG, Wicki J, Fantur K, Paschke E, Mahuran DJ, Rigat BA, Tropak M, Wrodnigg TM. Synthesis of lipophilic 1-deoxygalactonojirimycin derivatives as D-galactosidase inhibitors. Beilstein Journal of Organic Chemistry. 6: 21. PMID 20502610 DOI: 10.3762/bjoc.6.21  1
2010 Fröhlich RF, Furneaux RH, Mahuran DJ, Rigat BA, Stütz AE, Tropak MB, Wicki J, Withers SG, Wrodnigg TM. 1-Deoxynojirimycins with dansyl capped N-substituents as probes for Morbus Gaucher affected cell lines. Carbohydrate Research. 345: 1371-6. PMID 20471633 DOI: 10.1016/j.carres.2010.04.015  1
2010 Kittl R, Withers SG. New approaches to enzymatic glycoside synthesis through directed evolution. Carbohydrate Research. 345: 1272-9. PMID 20427037 DOI: 10.1016/j.carres.2010.04.002  1
2010 Macdonald JM, Tarling CA, Taylor EJ, Dennis RJ, Myers DS, Knapp S, Davies GJ, Withers SG. Chitinase inhibition by chitobiose and chitotriose thiazolines. Angewandte Chemie (International Ed. in English). 49: 2599-602. PMID 20209544 DOI: 10.1002/anie.200906644  1
2010 Reitinger S, Yu Y, Wicki J, Ludwiczek M, D'Angelo I, Baturin S, Okon M, Strynadka NC, Lutz S, Withers SG, McIntosh LP. Circular permutation of Bacillus circulans xylanase: a kinetic and structural study. Biochemistry. 49: 2464-74. PMID 20163191 DOI: 10.1021/bi100036f  1
2010 Armstrong Z, Reitinger S, Kantner T, Withers SG. Enzymatic thioxyloside synthesis: characterization of thioglycoligase variants identified from a site-saturation mutagenesis library of Bacillus circulans xylanase. Chembiochem : a European Journal of Chemical Biology. 11: 533-8. PMID 20112321 DOI: 10.1002/cbic.200900711  1
2010 Zhang R, Yip VLY, Withers SG. Mechanisms of enzymatic glycosyl transfer Comprehensive Natural Products Ii: Chemistry and Biology. 8: 385-422.  1
2009 Steiner AJ, Schitter G, Stütz AE, Wrodnigg TM, Tarling CA, Withers SG, Mahuran DJ, Tropak MB. 2-Acetamino-1,2-dideoxynojirimycin-lysine hybrids as hexosaminidase inhibitors. Tetrahedron, Asymmetry. 20: 832-835. PMID 22328804 DOI: 10.1016/j.tetasy.2009.02.015  1
2009 Chan PH, Lairson LL, Lee HJ, Wakarchuk WW, Strynadka NC, Withers SG, McIntosh LP. NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base. Biochemistry. 48: 11220-30. PMID 19824695 DOI: 10.1021/bi901606n  1
2009 Rao FV, Rich JR, Rakić B, Buddai S, Schwartz MF, Johnson K, Bowe C, Wakarchuk WW, Defrees S, Withers SG, Strynadka NC. Structural insight into mammalian sialyltransferases. Nature Structural & Molecular Biology. 16: 1186-8. PMID 19820709 DOI: 10.1038/nsmb.1685  1
2009 Zhang R, Li C, Williams LK, Rempel BP, Brayer GD, Withers SG. Directed "in situ" inhibitor elongation as a strategy to structurally characterize the covalent glycosyl-enzyme intermediate of human pancreatic alpha-amylase. Biochemistry. 48: 10752-64. PMID 19803533 DOI: 10.1021/bi901400p  1
2009 Willis LM, Zhang R, Reid A, Withers SG, Wakarchuk WW. Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6. Biochemistry. 48: 10334-41. PMID 19788271 DOI: 10.1021/bi9013825  1
2009 Yang G, Withers SG. Ultrahigh-throughput FACS-based screening for directed enzyme evolution. Chembiochem : a European Journal of Chemical Biology. 10: 2704-15. PMID 19780076 DOI: 10.1002/cbic.200900384  1
2009 Kim JH, Ryu HW, Shim JH, Park KH, Withers SG. Development of new and selective Trypanosoma cruzi trans-sialidase inhibitors from sulfonamide chalcones and their derivatives. Chembiochem : a European Journal of Chemical Biology. 10: 2475-9. PMID 19780074 DOI: 10.1002/cbic.200900108  1
2009 Shaikh FA, Randriantsoa M, Withers SG. Mechanistic analysis of the blood group antigen-cleaving endo-beta-galactosidase from Clostridium perfringens. Biochemistry. 48: 8396-404. PMID 19630404 DOI: 10.1021/bi900991h  1
2009 Offen WA, Zechel DL, Withers SG, Gilbert HJ, Davies GJ. Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis. Chemical Communications (Cambridge, England). 2484-6. PMID 19532864 DOI: 10.1039/b902240f  1
2009 Hancock SM, Rich JR, Caines ME, Strynadka NC, Withers SG. Designer enzymes for glycosphingolipid synthesis by directed evolution. Nature Chemical Biology. 5: 508-14. PMID 19525967 DOI: 10.1038/nchembio.191  1
2009 Lee HJ, Rakić B, Gilbert M, Wakarchuk WW, Withers SG, Strynadka NC. Structural and kinetic characterizations of the polysialic acid O-acetyltransferase OatWY from Neisseria meningitidis. The Journal of Biological Chemistry. 284: 24501-11. PMID 19525232 DOI: 10.1074/jbc.M109.006049  1
2009 Morley TJ, Willis LM, Whitfield C, Wakarchuk WW, Withers SG. A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase. The Journal of Biological Chemistry. 284: 17404-10. PMID 19411257 DOI: 10.1074/jbc.M109.003970  1
2009 Rich JR, Withers SG. Emerging methods for the production of homogeneous human glycoproteins. Nature Chemical Biology. 5: 206-15. PMID 19295526 DOI: 10.1038/nchembio.148  1
2009 Rempel BP, Withers SG. Non-Stick sugars: Synthesis of difluorosugar fluorides as potential glycosidase inactivators Australian Journal of Chemistry. 62: 590-599. DOI: 10.1071/CH09223  1
2009 Rakić B, Withers SG. Recent developments in Glycoside Synthesis with Glycosynthases and Thioglycoligases Australian Journal of Chemistry. 62: 510-520. DOI: 10.1071/CH09059  1
2009 Steiner AJ, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. Synthesis and biological evaluation of 1,5-dideoxy-1,5-iminoxylitol-Aamino acid hybrids as xylosidase inhibitors Australian Journal of Chemistry. 62: 553-557. DOI: 10.1071/CH09040  1
2009 Davies GJ, Withers SG, Vocadlo DJ. The chitopentaose complex of a mutant en egg-white lysozyme displays no distortion of the -1 sugar away from a 4C1 chair conformation Australian Journal of Chemistry. 62: 528-532. DOI: 10.1071/CH09038  1
2009 Le QT, Lee CK, Kim YW, Lee SJ, Zhang R, Withers SG, Kim YR, Auh JH, Park KH. Amylolytically-resistant tapioca starch modified by combined treatment of branching enzyme and maltogenic amylase Carbohydrate Polymers. 75: 9-14. DOI: 10.1016/j.carbpol.2008.06.001  1
2008 Greig IR, Zahariev F, Withers SG. Elucidating the nature of the Streptomyces plicatus beta-hexosaminidase-bound intermediate using ab initio molecular dynamics simulations. Journal of the American Chemical Society. 130: 17620-8. PMID 19053474 DOI: 10.1021/ja805640c  1
2008 Steiner AJ, Schitter G, Stütz AE, Wrodnigg TM, Tarling CA, Withers SG, Fantur K, Mahuran D, Paschke E, Tropak M. 1-Deoxygalactonojirimycin-lysine hybrids as potent D-galactosidase inhibitors. Bioorganic & Medicinal Chemistry. 16: 10216-20. PMID 18996021 DOI: 10.1016/j.bmc.2008.10.054  1
2008 Caines ME, Zhu H, Vuckovic M, Willis LM, Withers SG, Wakarchuk WW, Strynadka NC. The structural basis for T-antigen hydrolysis by Streptococcus pneumoniae: a target for structure-based vaccine design. The Journal of Biological Chemistry. 283: 31279-83. PMID 18784084 DOI: 10.1074/jbc.C800150200  1
2008 Kuntz DA, Tarling CA, Withers SG, Rose DR. Structural analysis of Golgi alpha-mannosidase II inhibitors identified from a focused glycosidase inhibitor screen. Biochemistry. 47: 10058-68. PMID 18759458 DOI: 10.1021/bi8010785  1
2008 Hancock SM, Tarling CA, Withers SG. High-throughput screening of cell lysates for ganglioside synthesis. Analytical Biochemistry. 382: 48-54. PMID 18703011 DOI: 10.1016/j.ab.2008.07.023  1
2008 Hekmat O, He S, Warren RA, Withers SG. A mechanism-based ICAT strategy for comparing relative expression and activity levels of glycosidases in biological systems. Journal of Proteome Research. 7: 3282-92. PMID 18563928 DOI: 10.1021/pr7008302  1
2008 Lee YS, Lee MH, Lee HS, Lee SJ, Kim YW, Zhang R, Withers SG, Kim KS, Lee SJ, Park KH. Enzymatic synthesis of a selective inhibitor for alpha-glucosidases: alpha-acarviosinyl-(1-->9)-3-alpha-D-glucopyranosylpropen. Journal of Agricultural and Food Chemistry. 56: 5324-30. PMID 18553919 DOI: 10.1021/jf703655k  1
2008 Lairson LL, Henrissat B, Davies GJ, Withers SG. Glycosyltransferases: structures, functions, and mechanisms. Annual Review of Biochemistry. 77: 521-55. PMID 18518825 DOI: 10.1146/annurev.biochem.76.061005.092322  1
2008 Rempel BP, Withers SG. Covalent inhibitors of glycosidases and their applications in biochemistry and biology. Glycobiology. 18: 570-86. PMID 18499865 DOI: 10.1093/glycob/cwn041  1
2008 Namdjou DJ, Chen HM, Vinogradov E, Brochu D, Withers SG, Wakarchuk WW. A beta-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis. Chembiochem : a European Journal of Chemical Biology. 9: 1632-40. PMID 18491328 DOI: 10.1002/cbic.200700775  1
2008 Shaikh FA, Withers SG. Teaching old enzymes new tricks: engineering and evolution of glycosidases and glycosyl transferases for improved glycoside synthesis. Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. 86: 169-77. PMID 18443630 DOI: 10.1139/O07-149  1
2008 Amorelli B, Yang C, Rempel B, Withers SG, Knapp S. N-Acetylhexosaminidase inhibitory properties of C-1 homologated GlcNAc- and GalNAc-thiazolines. Bioorganic & Medicinal Chemistry Letters. 18: 2944-7. PMID 18406613 DOI: 10.1016/j.bmcl.2008.03.067  1
2008 Zhang R, McCarter JD, Braun C, Yeung W, Brayer GD, Withers SG. Synthesis and testing of 2-deoxy-2,2-dihaloglycosides as mechanism-based inhibitors of alpha-glycosidases. The Journal of Organic Chemistry. 73: 3070-7. PMID 18345685 DOI: 10.1021/jo702565q  1
2008 Wilkinson SM, Liew CW, Mackay JP, Salleh HM, Withers SG, McLeod MD. Escherichia coli glucuronylsynthase: an engineered enzyme for the synthesis of beta-glucuronides. Organic Letters. 10: 1585-8. PMID 18345681 DOI: 10.1021/ol8002767  1
2008 Buchini S, Buschiazzo A, Withers SG. A new generation of specific Trypanosoma cruzi trans-sialidase inhibitors. Angewandte Chemie (International Ed. in English). 47: 2700-3. PMID 18300214 DOI: 10.1002/anie.200705435  1
2008 Eltis LD, Withers SG. Nature teaches but can be bettered. Current Opinion in Chemical Biology. 12: 115-7. PMID 18291124 DOI: 10.1016/j.cbpa.2008.01.030  1
2008 Maurus R, Begum A, Williams LK, Fredriksen JR, Zhang R, Withers SG, Brayer GD. Alternative catalytic anions differentially modulate human alpha-amylase activity and specificity. Biochemistry. 47: 3332-44. PMID 18284212 DOI: 10.1021/bi701652t  1
2008 Damager I, Buchini S, Amaya MF, Buschiazzo A, Alzari P, Frasch AC, Watts A, Withers SG. Kinetic and mechanistic analysis of Trypanosoma cruzi trans-sialidase reveals a classical ping-pong mechanism with acid/base catalysis. Biochemistry. 47: 3507-12. PMID 18284211 DOI: 10.1021/bi7024832  1
2008 Steiner AJ, Stütz AE, Wrodnigg TM, Tarling CA, Withers SG, Hermetter A, Schmidinger H. Glycosidase profiling with immobilised glycosidase-inhibiting iminoalditols-A proof-of-concept study Bioorganic and Medicinal Chemistry Letters. 18: 1922-1925. PMID 18281216 DOI: 10.1016/j.bmcl.2008.01.124  1
2008 Newstead SL, Potter JA, Wilson JC, Xu G, Chien CH, Watts AG, Withers SG, Taylor GL. The structure of Clostridium perfringens NanI sialidase and its catalytic intermediates. The Journal of Biological Chemistry. 283: 9080-8. PMID 18218621 DOI: 10.1074/jbc.M710247200  1
2008 Tarling CA, Woods K, Zhang R, Brastianos HC, Brayer GD, Andersen RJ, Withers SG. The search for novel human pancreatic alpha-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts. Chembiochem : a European Journal of Chemical Biology. 9: 433-8. PMID 18214874 DOI: 10.1002/cbic.200700470  1
2008 Jamaluddin H, Tumbale P, Withers SG, Acharya KR, Brew K. Corrigendum to "Conformational Changes Induced by Binding of UDP-2F-galactose to α-1,3 Galactosyltransferase-Implications for Catalysis" [J. Mol. Biol. 369 (2007) 1270-1281] (DOI:10.1016/j.jmb.2007.04.012) Journal of Molecular Biology. 378: 295-296. DOI: 10.1016/j.jmb.2008.02.030  1
2008 Vocadlo DJ, Withers SG. Glycosidase-catalysed oligosaccharide synthesis Carbohydrates in Chemistry and Biology. 2: 723-844. DOI: 10.1002/9783527618255.ch29  1
2007 Hekmat O, Florizone C, Kim YW, Eltis LD, Warren RA, Withers SG. Specificity fingerprinting of retaining beta-1,4-glycanases in the Cellulomonas fimi secretome using two fluorescent mechanism-based probes. Chembiochem : a European Journal of Chemical Biology. 8: 2125-32. PMID 17952884 DOI: 10.1002/cbic.200700481  1
2007 Yip VL, Thompson J, Withers SG. Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4. Biochemistry. 46: 9840-52. PMID 17676871 DOI: 10.1021/bi700536p  1
2007 Kim YW, Chen HM, Kim JH, Müllegger J, Mahuran D, Withers SG. Thioglycoligase-based assembly of thiodisaccharides: Screening as β-galactosidase inhibitors Chembiochem. 8: 1495-1499. PMID 17661304 DOI: 10.1002/cbic.200700263  1
2007 Chen HM, Withers SG. Syntheses of the 3- and 4-thio analogues of 4-nitrophenyl 2-acetamido-2-deoxy-β-d-gluco- and galactopyranoside Carbohydrate Research. 342: 2212-2222. PMID 17612513 DOI: 10.1016/j.carres.2007.05.023  1
2007 Schubert M, Poon DK, Wicki J, Tarling CA, Kwan EM, Nielsen JE, Withers SG, McIntosh LP. Probing electrostatic interactions along the reaction pathway of a glycoside hydrolase: histidine characterization by NMR spectroscopy. Biochemistry. 46: 7383-95. PMID 17547373 DOI: 10.1021/bi700249m  1
2007 Chiu CP, Lairson LL, Gilbert M, Wakarchuk WW, Withers SG, Strynadka NC. Structural analysis of the alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni in apo and substrate-analogue bound forms. Biochemistry. 46: 7196-204. PMID 17518445 DOI: 10.1021/bi602543d  1
2007 Wicki J, Schloegl J, Tarling CA, Withers SG. Recruitment of both uniform and differential binding energy in enzymatic catalysis: Xylanases from families 10 and 11 Biochemistry. 46: 6996-7005. PMID 17503782 DOI: 10.1021/bi700359e  1
2007 Jamaluddin H, Tumbale P, Withers SG, Acharya KR, Brew K. Conformational Changes Induced by Binding UDP-2F-galactose to α-1,3 Galactosyltransferase- Implications for Catalysis Journal of Molecular Biology. 369: 1270-1281. PMID 17493636 DOI: 10.1016/j.jmb.2007.04.012  1
2007 Caines ME, Hancock SM, Tarling CA, Wrodnigg TM, Stick RV, Stütz AE, Vasella A, Withers SG, Strynadka NC. The structural basis of glycosidase inhibition by five-membered iminocyclitols: the clan a glycoside hydrolase endoglycoceramidase as a model system. Angewandte Chemie (International Ed. in English). 46: 4474-6. PMID 17487923 DOI: 10.1002/anie.200700268  1
2007 Shaikh FA, Müllegger J, He S, Withers SG. Identification of the catalytic nucleophile in Family 42 β-galactosidases by intermediate trapping and peptide mapping: YesZ from Bacillus subtilis Febs Letters. 581: 2441-2446. PMID 17485082 DOI: 10.1016/j.febslet.2007.04.053  1
2007 Steiner AJ, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. Iminoalditol-amino acid hybrids: synthesis and evaluation as glycosidase inhibitors Carbohydrate Research. 342: 1850-1858. PMID 17442281 DOI: 10.1016/j.carres.2007.03.024  1
2007 Daniels G, Withers SG. Towards universal red blood cells Nature Biotechnology. 25: 427-428. PMID 17420747 DOI: 10.1038/nbt0407-427  1
2007 Hommalai G, Withers SG, Chuenchor W, Cairns JRK, Svasti J. Enzymatic synthesis of cello-oligosaccharides by rice BGlu1 β-glucosidase glycosynthase mutants Glycobiology. 17: 744-753. PMID 17405771 DOI: 10.1093/glycob/cwm039  1
2007 Wicki J, Williams SJ, Withers SG. Transition-state mimicry by glycosidase inhibitors: a critical kinetic analysis. Journal of the American Chemical Society. 129: 4530-1. PMID 17385869 DOI: 10.1021/ja0707254  1
2007 Chen HM, Withers SG. Facile synthesis of 2,4-dinitrophenyl α-D-glycopyranosides as chromogenic substrates for α-glycosidases Chembiochem. 8: 719-722. PMID 17373018 DOI: 10.1002/cbic.200700021  1
2007 Caines ME, Vaughan MD, Tarling CA, Hancock SM, Warren RA, Withers SG, Strynadka NC. Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms. The Journal of Biological Chemistry. 282: 14300-8. PMID 17329247 DOI: 10.1074/jbc.M611455200  1
2007 Tropak MB, Blanchard JE, Withers SG, Brown ED, Mahuran D. High-Throughput Screening for Human Lysosomal β-N-Acetyl Hexosaminidase Inhibitors Acting as Pharmacological Chaperones Chemistry and Biology. 14: 153-164. PMID 17317569 DOI: 10.1016/j.chembiol.2006.12.006  1
2007 Poon DK, Ludwiczek ML, Schubert M, Kwan EM, Withers SG, McIntosh LP. NMR spectroscopic characterization of a beta-(1,4)-glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate. Biochemistry. 46: 1759-70. PMID 17253772 DOI: 10.1021/bi061694c  1
2007 Lairson LL, Wakarchuk WW, Withers SG. Alternative donor substrates for inverting and retaining glycosyltransferases Chemical Communications. 365-367. PMID 17220972 DOI: 10.1039/b614636h  1
2007 Krupa JC, Shaya D, Chi L, Linhardt RJ, Cygler M, Withers SG, Mort JS. Quantitative continuous assay for hyaluronan synthase. Analytical Biochemistry. 361: 218-25. PMID 17173853 DOI: 10.1016/j.ab.2006.11.011  1
2007 Poon DK, Withers SG, McIntosh LP. Direct demonstration of the flexibility of the glycosylated proline-threonine linker in the Cellulomonas fimi Xylanase Cex through NMR spectroscopic analysis. The Journal of Biological Chemistry. 282: 2091-100. PMID 17121820 DOI: 10.1074/jbc.M609670200  1
2007 Tesić M, Wicki J, Poon DK, Withers SG, Douglas DJ. Gas phase noncovalent protein complexes that retain solution binding properties: Binding of xylobiose inhibitors to the beta-1, 4 exoglucanase from cellulomonas fimi. Journal of the American Society For Mass Spectrometry. 18: 64-73. PMID 17005414 DOI: 10.1016/j.jasms.2006.08.012  1
2007 Meloncelli PJ, Gloster TM, Money VA, Tarling CA, Davies GJ, Withers SG, Stick RV. D-glucosylated derivatives of isofagomine and noeuromycin and their potential as inhibitors of β-glycoside hydrolases Australian Journal of Chemistry. 60: 549-565. DOI: 10.1071/CH07188  1
2007 Buchini S, Withers SG. Engineered glycosidases for glycoside synthesis Chimica Oggi. 25: 4-6.  1
2006 Poon DK, Schubert M, Au J, Okon M, Withers SG, McIntosh LP. Unambiguous determination of the ionization state of a glycoside hydrolase active site lysine by 1H-15N heteronuclear correlation spectroscopy. Journal of the American Chemical Society. 128: 15388-9. PMID 17132001 DOI: 10.1021/ja065766z  1
2006 Lairson LL, Watts AG, Wakarchuk WW, Withers SG. Using substrate engineering to harness enzymatic promiscuity and expand biological catalysis. Nature Chemical Biology. 2: 724-8. PMID 17057723 DOI: 10.1038/nchembio828  1
2006 Yip VL, Withers SG. Family 4 glycosidases carry out efficient hydrolysis of thioglycosides by an alpha,beta-elimination mechanism. Angewandte Chemie (International Ed. in English). 45: 6179-82. PMID 16917793 DOI: 10.1002/anie.200601421  1
2006 Hancock SM, Vaughan MD, Withers SG. Engineering of glycosidases and glycosyltransferases Current Opinion in Chemical Biology. 10: 509-519. PMID 16905354 DOI: 10.1016/j.cbpa.2006.07.015  1
2006 Blair DE, Hekmat O, Schüttelkopf AW, Shrestha B, Tokuyasu K, Withers SG, Van Aalten DMF. Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum Biochemistry. 45: 9416-9426. PMID 16878976 DOI: 10.1021/bi0606694  1
2006 Aharoni A, Thieme K, Chiu CP, Buchini S, Lairson LL, Chen H, Strynadka NC, Wakarchuk WW, Withers SG. High-throughput screening methodology for the directed evolution of glycosyltransferases. Nature Methods. 3: 609-14. PMID 16862135 DOI: 10.1038/nmeth899  1
2006 Kim YW, Chen H, Kim JH, Withers SG. Catalytic properties of a mutant β-galactosidase from Xanthomonas manihotis engineered to synthesize galactosyl-thio-β-1,3 and -β-1,4-glycosides Febs Letters. 580: 4377-4381. PMID 16844121 DOI: 10.1016/j.febslet.2006.06.095  1
2006 Müllegger J, Chen HM, Chan WY, Reid SP, Jahn M, Warren RAJ, Salleh HM, Withers SG. Thermostable glycosynthases and thioglycoligases derived from Thermotoga maritima β-glucuronidase Chembiochem. 7: 1028-1030. PMID 16795119 DOI: 10.1002/cbic.200600028  1
2006 Gordon RD, Sivarajah P, Satkunarajah M, Ma D, Tarling CA, Vizitiu D, Withers SG, Rini JM. X-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues. Journal of Molecular Biology. 360: 67-79. PMID 16769084 DOI: 10.1016/j.jmb.2006.04.058  1
2006 Mayer C, Vocadlo DJ, Mah M, Rupitz K, Stoll D, Warren RA, Withers SG. Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi. The Febs Journal. 273: 2929-41. PMID 16762038 DOI: 10.1111/j.1742-4658.2006.05308.x  1
2006 Lemieux MJ, Mark BL, Cherney MM, Withers SG, Mahuran DJ, James MN. Crystallographic structure of human beta-hexosaminidase A: interpretation of Tay-Sachs mutations and loss of GM2 ganglioside hydrolysis. Journal of Molecular Biology. 359: 913-29. PMID 16698036 DOI: 10.1016/j.jmb.2006.04.004  1
2006 Kim YW, Fox DT, Hekmat O, Kantner T, McIntosh LP, Warren RA, Withers SG. Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi. Organic & Biomolecular Chemistry. 4: 2025-32. PMID 16688347 DOI: 10.1039/b601667g  1
2006 Vaughan MD, Johnson K, DeFrees S, Tang X, Warren RA, Withers SG. Glycosynthase-mediated synthesis of glycosphingolipids. Journal of the American Chemical Society. 128: 6300-1. PMID 16683778 DOI: 10.1021/ja058469n  1
2006 Wrodnigg TM, Stütz AE, Tarling CA, Withers SG. Fine tuning of β-glucosidase inhibitory activity in the 2,5-dideoxy-2,5-imino-d-mannitol (DMDP) system Carbohydrate Research. 341: 1717-1722. PMID 16616905 DOI: 10.1016/j.carres.2006.03.010  1
2006 Müllegger J, Chen HM, Warren RAJ, Withers SG. Glycosylation of a neoglycoprotein by using glycosynthase and thioglycoligase approaches: The generation of a thioglycoprotein Angewandte Chemie - International Edition. 45: 2585-2588. PMID 16528765 DOI: 10.1002/anie.200503900  1
2006 Rye CS, Matte A, Cygler M, Withers SG. An atypical approach identifies TYR234 as the key base catalyst in chondroitin AC lyase. Chembiochem : a European Journal of Chemical Biology. 7: 631-7. PMID 16521140 DOI: 10.1002/cbic.200500428  1
2006 Yip VL, Withers SG. Breakdown of oligosaccharides by the process of elimination. Current Opinion in Chemical Biology. 10: 147-55. PMID 16495121 DOI: 10.1016/j.cbpa.2006.02.005  1
2006 Greimel P, Häusler H, Lundt I, Rupitz K, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. Fluorescent glycosidase inhibiting 1,5-dideoxy-1,5-iminoalditols Bioorganic and Medicinal Chemistry Letters. 16: 2067-2070. PMID 16481162 DOI: 10.1016/j.bmcl.2006.01.095  1
2006 Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG. Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex. Journal of the American Chemical Society. 128: 2202-3. PMID 16478160 DOI: 10.1021/ja057904a  1
2006 Yip VL, Withers SG. Mechanistic analysis of the unusual redox-elimination sequence employed by Thermotoga maritima BglT: a 6-phospho-beta-glucosidase from glycoside hydrolase family 4. Biochemistry. 45: 571-80. PMID 16401086 DOI: 10.1021/bi052054x  1
2006 Williams SJ, Hekmat O, Withers SG. Synthesis and testing of mechanism-based protein-profiling probes for retaining endo-glycosidases. Chembiochem : a European Journal of Chemical Biology. 7: 116-24. PMID 16397879 DOI: 10.1002/cbic.200500279  1
2006 Salleh HM, Müllegger J, Reid SP, Chan WY, Hwang J, Warren RA, Withers SG. Cloning and characterization of Thermotoga maritima beta-glucuronidase. Carbohydrate Research. 341: 49-59. PMID 16303119 DOI: 10.1016/j.carres.2005.10.005  1
2006 Watts AG, Oppezzo P, Withers SG, Alzari PM, Buschiazzo A. Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase. The Journal of Biological Chemistry. 281: 4149-55. PMID 16298994 DOI: 10.1074/jbc.M510677200  1
2006 Lundt I, Steiner AJ, Stütz AE, Tarling CA, Ully S, Withers SG, Wrodnigg TM. Fluorescently tagged iminoalditol glycosidase inhibitors as novel biological probes and diagnostics Bioorganic and Medicinal Chemistry. 14: 1737-1742. PMID 16274997 DOI: 10.1016/j.bmc.2005.10.021  1
2006 Yip VL, Withers SG. Family 4 glycoside hydrolases are special: The first β-elimination mechanism amongst glycoside hydrolases Biocatalysis and Biotransformation. 24: 167-176. DOI: 10.1080/10242420500515926  1
2006 Tauss A, Steiner AJ, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. L-Idoseptanosides: Substrates of D-glucosidases? Tetrahedron Asymmetry. 17: 234-239. DOI: 10.1016/j.tetasy.2005.12.007  1
2006 Mildvan AS, Xia Z, Azurmendi HF, Legler PM, Balfour MR, Lairson LL, Withers SG, Gabelli SB, Bianchet MA, Amzel LM. Hydrogen bonding in the mechanism of GDP-mannose mannosyl hydrolase Journal of Molecular Structure. 790: 160-167. DOI: 10.1016/j.molstruc.2005.09.024  1
2006 Pabba J, Rempel BP, Withers SG, Vasella A. Synthesis of glycaro-1,5-lactams and tetrahydrotetrazolopyridine-5- carboxylates: Inhibitors of β-D-glucuronidase and α-L-iduronidase Helvetica Chimica Acta. 89: 635-666. DOI: 10.1002/hlca.200690066  1
2005 Kim YW, Chen H, Withers SG. Enzymatic transglycosylation of xylose using a glycosynthase Carbohydrate Research. 340: 2735-2741. PMID 16259970 DOI: 10.1016/j.carres.2005.09.010  1
2005 Duncan G, Tarling CA, Bingle WH, Nomellini JF, Yamage M, Dorocicz IR, Withers SG, Smit J. Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus: fusion with the beta-1,4-glycanase (Cex) from the cellulolytic bacterium Cellulomonas fimi yields a robust, catalytically active product. Applied Biochemistry and Biotechnology. 127: 95-110. PMID 16258187 DOI: 10.1385/ABAB:127:2:095  1
2005 Vocadlo DJ, Withers SG. Detailed comparative analysis of the catalytic mechanisms of beta-N-acetylglucosaminidases from families 3 and 20 of glycoside hydrolases. Biochemistry. 44: 12809-18. PMID 16171396 DOI: 10.1021/bi051121k  1
2005 Knapp S, Yang C, Pabbaraja S, Rempel B, Reid S, Withers SG. Synthesis and kinetic analysis of the N-acetylhexosaminidase inhibitor XylNAc-isofagomine. The Journal of Organic Chemistry. 70: 7715-20. PMID 16149804 DOI: 10.1021/jo051117e  0.32
2005 Hekmat O, Kim YW, Williams SJ, He S, Withers SG. Active-site peptide "fingerprinting" of glycosidases in complex mixtures by mass spectrometry. Discovery of a novel retaining beta-1,4-glycanase in Cellulomonas fimi. The Journal of Biological Chemistry. 280: 35126-35. PMID 16085650 DOI: 10.1074/jbc.M508434200  1
2005 Xia Z, Azurmendi HF, Lairson LL, Withers SG, Gabelli SB, Bianchet MA, Amzel LM, Mildvan AS. Mutational, structural, and kinetic evidence for a dissociative mechanism in the GDP-mannose mannosyl hydrolase reaction. Biochemistry. 44: 8989-97. PMID 15966723 DOI: 10.1021/bi050583v  1
2005 Rempel BP, Clarke LA, Withers SG. A homology model for human α-L-iduronidase: Insights into human disease Molecular Genetics and Metabolism. 85: 28-37. PMID 15862278 DOI: 10.1016/j.ymgme.2004.12.006  1
2005 Müllegger J, Jahn M, Chen HM, Warren RA, Withers SG. Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts. Protein Engineering, Design & Selection : Peds. 18: 33-40. PMID 15790578 DOI: 10.1093/protein/gzi003  0.44
2005 Li C, Begum A, Numao S, Park KH, Withers SG, Brayer GD. Acarbose rearrangement mechanism implied by the kinetic and structural analysis of human pancreatic alpha-amylase in complex with analogues and their elongated counterparts. Biochemistry. 44: 3347-57. PMID 15736945 DOI: 10.1021/bi048334e  1
2005 Maurus R, Begum A, Kuo HH, Racaza A, Numao S, Andersen C, Tams JW, Vind J, Overall CM, Withers SG, Brayer GD. Structural and mechanistic studies of chloride induced activation of human pancreatic alpha-amylase. Protein Science : a Publication of the Protein Society. 14: 743-55. PMID 15722449 DOI: 10.1110/ps.041079305  1
2005 Vocadlo DJ, Withers SG. The chemical synthesis of 2-deoxy-2-fluorodisaccharide probes of the hen egg white lysozyme mechanism. Carbohydrate Research. 340: 379-88. PMID 15680592 DOI: 10.1016/j.carres.2004.12.015  1
2005 Varrot A, Yip VL, Li Y, Rajan SS, Yang X, Anderson WF, Thompson J, Withers SG, Davies GJ. NAD+ and metal-ion dependent hydrolysis by family 4 glycosidases: structural insight into specificity for phospho-beta-D-glucosides. Journal of Molecular Biology. 346: 423-35. PMID 15670594 DOI: 10.1016/j.jmb.2004.11.058  1
2005 Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC. Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate. The Journal of Biological Chemistry. 280: 2105-15. PMID 15501829 DOI: 10.1074/jbc.M410468200  1
2005 Lee SS, Yu S, Withers SG. Mechanism of action of exo-acting α-1,4-glucan lyase: A glycoside hydrolase family 31 enzyme Biologia - Section Cellular and Molecular Biology. 60: 137-148.  1
2004 Lairson LL, Withers SG. Mechanistic analogies amongst carbohydrate modifying enzymes. Chemical Communications (Cambridge, England). 2243-8. PMID 15489968 DOI: 10.1039/b406490a  0.8
2004 Yip VL, Withers SG. Nature's many mechanisms for the degradation of oligosaccharides. Organic & Biomolecular Chemistry. 2: 2707-13. PMID 15455137 DOI: 10.1039/B408880H  1
2004 Gloster TM, Macdonald JM, Tarling CA, Stick RV, Withers SG, Davies GJ. Structural, thermodynamic, and kinetic analyses of tetrahydrooxazine-derived inhibitors bound to beta-glucosidases. The Journal of Biological Chemistry. 279: 49236-42. PMID 15356002 DOI: 10.1074/jbc.M407195200  1
2004 Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF. Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis. Structure (London, England : 1993). 12: 1619-29. PMID 15341727 DOI: 10.1016/j.str.2004.06.020  1
2004 Gloster TM, Williams SJ, Roberts S, Tarling CA, Wicki J, Withers SG, Davies GJ. Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A. Chemical Communications (Cambridge, England). 1794-5. PMID 15306887 DOI: 10.1039/b405152a  1
2004 Numao S, Damager I, Li C, Wrodnigg TM, Begum A, Overall CM, Brayer GD, Withers SG. In situ extension as an approach for identifying novel alpha-amylase inhibitors. The Journal of Biological Chemistry. 279: 48282-91. PMID 15304511 DOI: 10.1074/jbc.M406804200  1
2004 Yip VL, Varrot A, Davies GJ, Rajan SS, Yang X, Thompson J, Anderson WF, Withers SG. An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima. Journal of the American Chemical Society. 126: 8354-5. PMID 15237973 DOI: 10.1021/ja047632w  1
2004 Damager I, Numao S, Chen H, Brayer GD, Withers SG. Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase. Carbohydrate Research. 339: 1727-37. PMID 15220082 DOI: 10.1016/j.carres.2004.05.019  1
2004 Watts AG, Withers SG. Glycosynthase-catalysed formation of modified polysaccharide microstructures . The Biochemical Journal. 380: e9-10. PMID 15182233 DOI: 10.1042/BJ20040633  1
2004 Amaya MF, Watts AG, Damager I, Wehenkel A, Nguyen T, Buschiazzo A, Paris G, Frasch AC, Withers SG, Alzari PM. Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase. Structure (London, England : 1993). 12: 775-84. PMID 15130470 DOI: 10.1016/j.str.2004.02.036  1
2004 Lairson LL, Chiu CP, Ly HD, He S, Wakarchuk WW, Strynadka NC, Withers SG. Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue. The Journal of Biological Chemistry. 279: 28339-44. PMID 15075344 DOI: 10.1074/jbc.M400451200  1
2004 Rye CS, Withers SG. The synthesis of a novel thio-linked disaccharide of chondroitin as a potential inhibitor of polysaccharide lyases. Carbohydrate Research. 339: 699-703. PMID 15013408 DOI: 10.1016/j.carres.2003.12.011  1
2004 Chiu CP, Watts AG, Lairson LL, Gilbert M, Lim D, Wakarchuk WW, Withers SG, Strynadka NC. Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog. Nature Structural & Molecular Biology. 11: 163-70. PMID 14730352 DOI: 10.1038/nsmb720  1
2004 Sulzenbacher G, Bignon C, Nishimura T, Tarling CA, Withers SG, Henrissat B, Bourne Y. Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis. The Journal of Biological Chemistry. 279: 13119-28. PMID 14715651 DOI: 10.1074/jbc.M313783200  0.48
2004 Yang JK, Yoon HJ, Ahn HJ, Lee BI, Pedelacq JD, Liong EC, Berendzen J, Laivenieks M, Vieille C, Zeikus GJ, Vocadlo DJ, Withers SG, Suh SW. Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase. Journal of Molecular Biology. 335: 155-65. PMID 14659747 DOI: 10.1016/j.jmb.2003.10.026  1
2004 Watts AG, Withers SG. The synthesis of some mechanistic probes for sialic acid processing enzymes and the labeling of a sialidase from Trypanosoma rangeli Canadian Journal of Chemistry. 82: 1581-1588. DOI: 10.1139/v04-125  1
2003 Tarling CA, He S, Sulzenbacher G, Bignon C, Bourne Y, Henrissat B, Withers SG. Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis. The Journal of Biological Chemistry. 278: 47394-9. PMID 12975375 DOI: 10.1074/jbc.M306610200  0.48
2003 Numao S, Kuntz DA, Withers SG, Rose DR. Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates. The Journal of Biological Chemistry. 278: 48074-83. PMID 12960159 DOI: 10.1074/jbc.M309249200  1
2003 Ducros VM, Tarling CA, Zechel DL, Brzozowski AM, Frandsen TP, von Ossowski I, Schülein M, Withers SG, Davies GJ. Anatomy of glycosynthesis: structure and kinetics of the Humicola insolens Cel7B E197A and E197S glycosynthase mutants. Chemistry & Biology. 10: 619-28. PMID 12890535 DOI: 10.1016/S1074-5521(03)00143-1  1
2003 Jahn M, Stoll D, Warren RA, Szabó L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG. Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides. Chemical Communications (Cambridge, England). 1327-9. PMID 12841226 DOI: 10.1039/B302380J  1
2003 Nieman CE, Wong AW, He S, Clarke L, Hopwood JJ, Withers SG. Family 39 alpha-l-iduronidases and beta-D-xylosidases react through similar glycosyl-enzyme intermediates: identification of the human iduronidase nucleophile. Biochemistry. 42: 8054-65. PMID 12834357 DOI: 10.1021/bi034293v  0.36
2003 Watts AG, Damager I, Amaya ML, Buschiazzo A, Alzari P, Frasch AC, Withers SG. Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: tyrosine is the catalytic nucleophile. Journal of the American Chemical Society. 125: 7532-3. PMID 12812490 DOI: 10.1021/ja0344967  1
2003 Varrot A, Tarling CA, Macdonald JM, Stick RV, Zechel DL, Withers SG, Davies GJ. Direct observation of the protonation state of an imino sugar glycosidase inhibitor upon binding. Journal of the American Chemical Society. 125: 7496-7. PMID 12812472 DOI: 10.1021/ja034917k  1
2003 Zechel DL, Reid SP, Stoll D, Nashiru O, Warren RA, Withers SG. Mechanism, mutagenesis, and chemical rescue of a beta-mannosidase from cellulomonas fimi. Biochemistry. 42: 7195-204. PMID 12795616 DOI: 10.1021/bi034329j  1
2003 Gloster T, Williams SJ, Tarling CA, Roberts S, Dupont C, Jodoin P, Shareck F, Withers SG, Davies GJ. A xylobiose-derived isofagomine lactam glycosidase inhibitor binds as its amide tautomer. Chemical Communications (Cambridge, England). 944-5. PMID 12744311  1
2003 Poon DK, Webster P, Withers SG, McIntosh LP. Characterizing the pH-dependent stability and catalytic mechanism of the family 11 xylanase from the alkalophilic Bacillus agaradhaerens. Carbohydrate Research. 338: 415-21. PMID 12559743 DOI: 10.1016/S0008-6215(02)00486-X  1
2002 Withers SG, Wakarchuk WW, Strynadka NC. One step closer to a sweet conclusion. Chemistry & Biology. 9: 1270-3. PMID 12498879 DOI: 10.1016/S1074-5521(02)00291-0  0.68
2002 Rye CS, Withers SG. Development of an assay and determination of kinetic parameters for chondroitin AC lyase using defined synthetic substrates. Analytical Biochemistry. 308: 77-82. PMID 12234466 DOI: 10.1016/S0003-2697(02)00223-3  1
2002 Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabó L, Stoll D, Withers SG, Davies GJ. Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state. Angewandte Chemie (International Ed. in English). 41: 2824-7. PMID 12203498 DOI: 10.1002/1521-3773(20020802)41:15<2824::AID-ANIE2824>3.0.CO;2-G  1
2002 Rye CS, Withers SG. Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum. Journal of the American Chemical Society. 124: 9756-67. PMID 12175234 DOI: 10.1021/ja020627c  1
2002 Williams SJ, Mark BL, Vocadlo DJ, James MN, Withers SG. Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The Journal of Biological Chemistry. 277: 40055-65. PMID 12171933 DOI: 10.1074/jbc.M206481200  1
2002 Vocadlo DJ, Wicki J, Rupitz K, Withers SG. A case for reverse protonation: identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum beta-xylosidase and detailed kinetic analysis of a site-directed mutant. Biochemistry. 41: 9736-46. PMID 12146939  0.96
2002 Vocadlo DJ, Wicki J, Rupitz K, Withers SG. Mechanism of Thermoanaerobacterium saccharolyticum beta-xylosidase: kinetic studies. Biochemistry. 41: 9727-35. PMID 12146938  0.96
2002 Rye CS, Withers SG. Synthesis and evaluation of potential inhibitors of chondroitin AC lyase from Flavobacterium heparinum. The Journal of Organic Chemistry. 67: 4505-12. PMID 12076149 DOI: 10.1021/jo020089m  1
2002 Rydberg EH, Li C, Maurus R, Overall CM, Brayer GD, Withers SG. Mechanistic analyses of catalysis in human pancreatic alpha-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids. Biochemistry. 41: 4492-502. PMID 11914097 DOI: 10.1021/bi011821z  1
2002 Numao S, Maurus R, Sidhu G, Wang Y, Overall CM, Brayer GD, Withers SG. Probing the role of the chloride ion in the mechanism of human pancreatic alpha-amylase. Biochemistry. 41: 215-25. PMID 11772019 DOI: 10.1021/bi0115636  1
2002 Macdonald JM, Stick RV, Tilbrook DMG, Withers SG. Synthesis with glycosynthases: Cello-oligomers of isofagomine and a tetrahydrooxazine as cellulase inhibitors Australian Journal of Chemistry. 55: 747-752. DOI: 10.1071/CH02165  1
2001 Zechel DL, Withers SG. Dissection of nucleophilic and acid-base catalysis in glycosidases Current Opinion in Chemical Biology. 5: 643-649. PMID 11738173 DOI: 10.1016/S1367-5931(01)00260-5  1
2001 Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW. A structural view of the action of Escherichia coli (lacZ) beta-galactosidase. Biochemistry. 40: 14781-94. PMID 11732897 DOI: 10.1021/bi011727i  1
2001 Brun E, Brumer H, MacKenzie LF, Withers SG, McIntosh LP. Assignment of selectively 13C-labeled cellopentaose synthesized using an engineered glycosynthase [2] Journal of Biomolecular Nmr. 21: 67-68. PMID 11693570 DOI: 10.1023/A:1011927400439  1
2001 Mark BL, Vocadlo DJ, Zhao D, Knapp S, Withers SG, James MN. Biochemical and structural assessment of the 1-N-azasugar GalNAc-isofagomine as a potent family 20 beta-N-acetylhexosaminidase inhibitor. The Journal of Biological Chemistry. 276: 42131-7. PMID 11522797 DOI: 10.1074/jbc.M107154200  0.96
2001 Vocadlo DJ, Davies GJ, Laine R, Withers SG. Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate. Nature. 412: 835-8. PMID 11518970 DOI: 10.1038/35090602  1
2001 Joshi MD, Sidhu G, Nielsen JE, Brayer GD, Withers SG, McIntosh LP. Dissecting the electrostatic interactions and pH-dependent activity of a family 11 glycosidase Biochemistry. 40: 10115-10139. PMID 11513590 DOI: 10.1021/bi0105429  1
2001 Zechel DL, Reid SP, Nashiru O, Mayer C, Stoll D, Jakeman DL, Warren RAJ, Withers SG. Enzymatic synthesis of carbon-fluorine bonds [8] Journal of the American Chemical Society. 123: 4350-4351. PMID 11457208 DOI: 10.1021/ja005855q  1
2001 Blanchard JE, Gal L, He S, Foisy J, Warren RA, Withers SG. The identification of the catalytic nucleophiles of two beta-galactosidases from glycoside hydrolase family 35. Carbohydrate Research. 333: 7-17. PMID 11423106 DOI: 10.1016/S0008-6215(01)00108-2  0.36
2001 Hou Y, Vocadlo DJ, Leung A, Withers SG, Mahuran D. Characterization of the Glu and Asp residues in the active site of human beta-hexosaminidase B. Biochemistry. 40: 2201-9. PMID 11329289  0.96
2001 Persson K, Ly HD, Dieckelmann M, Wakarchuk WW, Withers SG, Strynadka NC. Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs. Nature Structural Biology. 8: 166-75. PMID 11175908 DOI: 10.1038/84168  0.68
2001 Mark BL, Vocadlo DJ, Knapp S, Triggs-Raine BL, Withers SG, James MN. Crystallographic evidence for substrate-assisted catalysis in a bacterial beta-hexosaminidase. The Journal of Biological Chemistry. 276: 10330-7. PMID 11124970 DOI: 10.1074/jbc.M011067200  0.96
2000 Ly HD, Howard S, Shum K, He S, Zhu A, Withers SG. The synthesis, testing and use of 5-fluoro-alpha-D-galactosyl fluoride to trap an intermediate on green coffee bean alpha-galactosidase and identify the catalytic nucleophile. Carbohydrate Research. 329: 539-47. PMID 11128583 DOI: 10.1016/S0008-6215(00)00214-7  0.36
2000 Numao S, He S, Evjen G, Howard S, Tollersrud OK, Withers SG. Identification of Asp197 as the catalytic nucleophile in the family 38 alpha-mannosidase from bovine kidney lysosomes. Febs Letters. 484: 175-8. PMID 11078873 DOI: 10.1016/S0014-5793(00)02148-7  1
2000 Rye CS, Withers SG. Glycosidase mechanisms. Current Opinion in Chemical Biology. 4: 573-80. PMID 11006547 DOI: 10.1016/S1367-5931(00)00135-6  1
2000 Notenboom V, Williams SJ, Hoos R, Withers SG, Rose DR. Detailed structural analysis of glycosidase/inhibitor interactions: complexes of Cex from Cellulomonas fimi with xylobiose-derived aza-sugars. Biochemistry. 39: 11553-63. PMID 10995222  0.88
2000 Williams SJ, Withers SG. Glycosyl fluorides in enzymatic reactions. Carbohydrate Research. 327: 27-46. PMID 10968675 DOI: 10.1016/S0008-6215(00)00041-0  1
2000 Vocadlo DJ, Withers SG. Identification of active site residues in glycosidases by use of tandem mass spectrometry. Methods in Molecular Biology (Clifton, N.J.). 146: 203-22. PMID 10948504 DOI: 10.1385/1-59259-045-4:203  0.96
2000 Hart DO, He S, Chany CJ, Withers SG, Sims PF, Sinnott ML, Brumer H. Identification of Asp-130 as the catalytic nucleophile in the main alpha-galactosidase from Phanerochaete chrysosporium, a family 27 glycosyl hydrolase. Biochemistry. 39: 9826-36. PMID 10933800 DOI: 10.1021/bi0008074  1
2000 Joshi MD, Sidhu G, Pot I, Brayer GD, Withers SG, McIntosh LP. Hydrogen bonding and catalysis: A novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase Journal of Molecular Biology. 299: 255-279. PMID 10860737 DOI: 10.1006/jmbi.2000.3722  1
2000 Brayer GD, Sidhu G, Maurus R, Rydberg EH, Braun C, Wang Y, Nguyen NT, Overall CM, Withers SG. Subsite mapping of the human pancreatic alpha-amylase active site through structural, kinetic, and mutagenesis techniques. Biochemistry. 39: 4778-91. PMID 10769135 DOI: 10.1021/bi9921182  1
2000 Connelly GP, Withers SG, McIntosh LP. Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate Protein Science. 9: 512-524. PMID 10752613 DOI: 10.1110/ps.9.3.512  1
2000 Dan S, Marton I, Dekel M, Bravdo BA, He S, Withers SG, Shoseyov O. Cloning, expression, characterization, and nucleophile identification of family 3, Aspergillus niger β-glucosidase Journal of Biological Chemistry. 275: 4973-4980. PMID 10671536 DOI: 10.1074/jbc.275.7.4973  1
2000 Mayer C, Zechel DL, Reid SP, Warren RAJ, Withers SG. The E358S mutant of Agrobacterium sp. β-glucosidase is a greatly improved glycosynthase Febs Letters. 466: 40-44. PMID 10648808 DOI: 10.1016/S0014-5793(99)01751-2  1
2000 Zechel DL, Withers SG. Glycosidase mechanisms: Anatomy of a finely tuned catalyst Accounts of Chemical Research. 33: 11-18. PMID 10639071 DOI: 10.1021/ar970172+  1
2000 Vocadlo DJ, Mayer C, He S, Withers SG. Mechanism of action and identification of Asp242 as the catalytic nucleophile of Vibrio furnisii N-acetyl-beta-D-glucosaminidase using 2-acetamido-2-deoxy-5-fluoro-alpha-L-idopyranosyl fluoride. Biochemistry. 39: 117-26. PMID 10625486  0.96
2000 Fairweather JK, Stick RV, Withers SG. A 'glycosynthase'-assisted synthesis of some epoxyalkyl β-C-oligosaccharides Australian Journal of Chemistry. 53: 913-916.  1
1999 Uitdehaag JCM, Mosi R, Kalk KH, Van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW. X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the α-amylase family Nature Structural Biology. 6: 432-436. PMID 10331869 DOI: 10.1038/8235  1
1999 Sidhu G, Withers SG, Nguyen NT, McIntosh LP, Ziser L, Brayer GD. Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase Biochemistry. 38: 5346-5354. PMID 10220321 DOI: 10.1021/bi982946f  1
1999 Johnson PE, Brun E, MacKenzie LF, Withers SG, McIntosh LP. The cellulose-binding domains from cellulomonas fimi β-1,4-glucanase CenC bind nitroxide spin-labeled cellooligosaccharides in multiple orientations Journal of Molecular Biology. 287: 609-625. PMID 10092463 DOI: 10.1006/jmbi.1999.2627  1
1999 Rydberg EH, Sidhu G, Vo HC, Hewitt J, Côte HC, Wang Y, Numao S, MacGillivray RT, Overall CM, Brayer GD, Withers SG. Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris. Protein Science : a Publication of the Protein Society. 8: 635-43. PMID 10091666 DOI: 10.1110/ps.8.3.635  1
1998 Mosi R, Sham H, Uitdehaag JCM, Ruiterkamp R, Dijkstra BW, Withers SG. Reassessment of acarbose as a transition state analogue inhibitor of cyclodextrin glycosyltransferase Biochemistry. 37: 17192-17198. PMID 9860832 DOI: 10.1021/bi981109a  1
1998 Wong AW, He S, Grubb JH, Sly WS, Withers SG. Identification of Glu-540 as the catalytic nucleophile of human beta-glucuronidase using electrospray mass spectrometry. The Journal of Biological Chemistry. 273: 34057-62. PMID 9852062  0.36
1998 Zechel DL, He S, Dupont C, Withers SG. Identification of glu-120 as the catalytic nucleophile in streptomyces lividans endoglucanase CelB Biochemical Journal. 336: 139-145. PMID 9806895 DOI: 10.1042/bj3360139  1
1998 Vocadlo DJ, MacKenzie LF, He S, Zeikus GJ, Withers SG. Identification of glu-277 as the catalytic nucleophile of Thermoanaerobacterium saccharolyticum beta-xylosidase using electrospray MS. The Biochemical Journal. 335: 449-55. PMID 9761746  0.96
1998 Notenboom V, Birsan C, Nitz M, Rose DR, Warren RA, Withers SG. Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants. Nature Structural Biology. 5: 812-8. PMID 9731776 DOI: 10.1038/1852  1
1998 Birsan C, Johnson P, Joshi M, MacLeod A, McIntosh L, Monem V, Nitz M, Rose DR, Tull D, Wakarchuck WW, Wang Q, Warren RA, White A, Withers SG. Mechanisms of cellulases and xylanases. Biochemical Society Transactions. 26: 156-60. PMID 9649739 DOI: 10.1042/bst0260156  1
1998 Zechel DL, Konermann L, Withers SG, Douglas DJ. Pre-steady state kinetic analysis of an enzymatic reaction monitored by time-resolved electrospray ionization mass spectrometry Biochemistry. 37: 7664-7669. PMID 9601025 DOI: 10.1021/bi980445o  1
1998 Notenboom V, Birsan C, Warren RA, Withers SG, Rose DR. Exploring the cellulose/xylan specificity of the beta-1,4-glycanase cex from Cellulomonas fimi through crystallography and mutation. Biochemistry. 37: 4751-8. PMID 9537990 DOI: 10.1021/bi9729211  0.4
1998 Lawson SL, Warren RA, Withers SG. Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue. The Biochemical Journal. 330: 203-9. PMID 9461511 DOI: 10.1042/bj3300203  1
1998 Ferro V, Weiler L, Withers SG. Convergent synthesis of a fluorescence-quenched glycopeptide as a potential substrate for peptide: N-glycosidases Carbohydrate Research. 306: 531-538. DOI: 10.1016/S0008-6215(98)00011-1  1
1997 Howard S, Braun C, McCarter J, Moremen KW, Liao YF, Withers SG. Human lysosomal and jack bean α-mannosidases are retaining glycosidases Biochemical and Biophysical Research Communications. 238: 896-898. PMID 9325188 DOI: 10.1006/bbrc.1997.7148  1
1997 Mosi R, He S, Uitdehaag J, Dijkstra BW, Withers SG. Trapping and characterization of the reaction intermediate in cyclodextrin glycosyltransferase by use of activated substrates and a mutant enzyme Biochemistry. 36: 9927-9934. PMID 9245426 DOI: 10.1021/bi970618u  1
1997 Lawson SL, Wakarchuk WW, Withers SG. Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase. Biochemistry. 36: 2257-65. PMID 9047328 DOI: 10.1021/bi9620215  1
1997 Mackenzie LF, Brooke GS, Cutfield JF, Sullivan PA, Withers SG. Identification of Glu-330 as the catalytic nucleophile of Candida albicans exo-beta-(1,3)-glucanase. The Journal of Biological Chemistry. 272: 3161-7. PMID 9013549 DOI: 10.1074/jbc.272.6.3161  1
1997 McCarter JD, Yeung W, Chow J, Dolphin D, Withers SG. Design and synthesis of 2'-deoxy-2'-fluorodisaccharides as mechanism- based glycosidase inhibitors that exploit aglycon specificity Journal of the American Chemical Society. 119: 5792-5797. DOI: 10.1021/ja9627454  1
1996 Lai EC, Morris SA, Street IP, Withers SG. Substituted glycals as probes of glycosidase mechanisms. Bioorganic & Medicinal Chemistry. 4: 1929-37. PMID 9007277 DOI: 10.1016/S0968-0896(96)00175-7  0.36
1996 MacLeod AM, Tull D, Rupitz K, Warren RA, Withers SG. Mechanistic consequences of mutation of active site carboxylates in a retaining beta-1,4-glycanase from Cellulomonas fimi. Biochemistry. 35: 13165-72. PMID 8855954 DOI: 10.1021/bi9610616  1
1996 Lawson SL, Wakarchuk WW, Withers SG. Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity. Biochemistry. 35: 10110-8. PMID 8756474 DOI: 10.1021/bi960586v  1
1996 McIntosh LP, Hand G, Johnson PE, Joshi MD, Korner M, Plesniak LA, Ziser L, Wakarchuk WW, Withers SG. The pK(a) of the general acid/base carboxyl group of a glycosidase cycles during catalysis: A 13C-NMR study of Bacillus circulans xylanase Biochemistry. 35: 9958-9966. PMID 8756457 DOI: 10.1021/bi9613234  1
1996 Tull D, Burgoyne DL, Chow DT, Withers SG, Aebersold R. A mass spectrometry-based approach for probing enzyme active sites: Identification of Glu 127 in Cellulomonas fimi exoglycanase as the residue modified by N-bromoacetyl cellobiosylamine Analytical Biochemistry. 234: 119-125. PMID 8714589 DOI: 10.1006/abio.1996.0063  1
1996 Mitchell EP, Withers SG, Ermert P, Vasella AT, Garman EF, Oikonomakos NG, Johnson LN. Ternary complex crystal structures of glycogen phosphorylase with the transition state analogue nojirimycin tetrazole and phosphate in the T and R states. Biochemistry. 35: 7341-55. PMID 8652510 DOI: 10.1021/bi960072w  1
1996 Braun C, Lindhorst T, Madsen NB, Withers SG. Identification of Asp 549 as the catalytic nucleophile of glycogen-debranching enzyme via trapping of the glycosyl-enzyme intermediate. Biochemistry. 35: 5458-63. PMID 8611536 DOI: 10.1021/bi9526488  1
1996 White A, Tull D, Johns K, Withers SG, Rose DR. Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase. Nature Structural Biology. 3: 149-54. PMID 8564541 DOI: 10.1038/nsb0296-149  1
1995 Tull D, Miao S, Withers SG, Aebersold R. Identification of derivatized peptides without radiolabels: tandem mass spectrometric localization of the tagged active-site nucleophiles of two cellulases and a beta-glucosidase. Analytical Biochemistry. 224: 509-14. PMID 7733452 DOI: 10.1006/abio.1995.1080  1
1995 Braun C, Withers SG. An assay for the transferase activity of glycogen debranching enzyme Carbohydrate Research. 271: 113-118. PMID 7648577 DOI: 10.1016/0008-6215(95)00020-T  1
1994 Tull D, Withers SG. Mechanisms of cellulases and xylanases: a detailed kinetic study of the exo-beta-1,4-glycanase from Cellulomonas fimi. Biochemistry. 33: 6363-70. PMID 8193153 DOI: 10.1021/bi00186a041  1
1994 Wang Q, Dechert U, Jirik F, Withers SG. Suicide inactivation of human prostatic acid phosphatase and a phosphotyrosine phosphatase Biochemical and Biophysical Research Communications. 200: 577-583. PMID 8166732 DOI: 10.1006/bbrc.1994.1487  1
1994 Miao S, Ziser L, Aebersold R, Withers SG. Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry Biochemistry. 33: 7027-7032. PMID 7911679 DOI: 10.1021/bi00189a002  1
1994 MacLeod AM, Lindhorst T, Withers SG, Warren RA. The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: evidence from detailed kinetic studies of mutants. Biochemistry. 33: 6371-6. PMID 7910761 DOI: 10.1021/bi00186a042  0.4
1993 Burk D, Wang Y, Dombroski D, Berghuis AM, Evans SV, Luo Y, Withers SG, Brayer GD. Isolation, crystallization and preliminary diffraction analyses of human pancreatic alpha-amylase. Journal of Molecular Biology. 230: 1084-5. PMID 8478920 DOI: 10.1006/jmbi.1993.1221  1
1993 McCarter JD, Adam MJ, Braun C, Namchuk M, Tull D, Withers SG. Syntheses of 2-deoxy-2-fluoro mono- and oligo-saccharide glycosides from glycals and evaluation as glycosidase inhibitors. Carbohydrate Research. 249: 77-90. PMID 8252556 DOI: 10.1016/0008-6215(93)84061-A  1
1993 Black TS, Kiss L, Tull D, Withers SG. N-bromoacetyl-glycopyranosylamines as affinity labels for a beta-glucosidase and a cellulase. Carbohydrate Research. 250: 195-202. PMID 8143291 DOI: 10.1016/0008-6215(93)84166-4  1
1992 Percival MD, Withers SG. 19F NMR investigations of the catalytic mechanism of phosphoglucomutase using fluorinated substrates and inhibitors. Biochemistry. 31: 505-12. PMID 1531026 DOI: 10.1021/bi00117a029  1
1992 Percival MD, Withers SG. Binding energy and catalysis: deoxyfluoro sugars as probes of hydrogen bonding in phosphoglucomutase. Biochemistry. 31: 498-505. PMID 1531025 DOI: 10.1021/bi00117a028  1
1992 Bedarkar S, Gilkes NR, Kilburn DG, Kwan E, Rose DR, Miller RC, Warren RA, Withers SG. Crystallization and preliminary X-ray diffraction analysis of the catalytic domain of Cex, an exo-beta-1,4-glucanase and beta-1,4-xylanase from the bacterium Cellulomonas fimi. Journal of Molecular Biology. 228: 693-5. PMID 1453471 DOI: 10.1016/0022-2836(92)90852-B  0.4
1992 Sprang SR, Madsen NB, Withers SG. Multiple phosphate positions in the catalytic site of glycogen phosphorylase: structure of the pyridoxal-5'-pyrophosphate coenzyme-substrate analog. Protein Science : a Publication of the Protein Society. 1: 1100-11. PMID 1304389 DOI: 10.1002/pro.5560010904  1
1991 Liu W, Madsen NB, Braun C, Withers SG. Reassessment of the catalytic mechanism of glycogen debranching enzyme. Biochemistry. 30: 1419-24. PMID 1991122  1
1991 Sprang SR, Withers SG, Goldsmith EJ, Fletterick RJ, Madsen NB. Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate. Science (New York, N.Y.). 254: 1367-71. PMID 1962195  1
1989 Withers SG, Percival MD, Street IP. The synthesis and hydrolysis of a series of deoxy- and deoxyfluoro-α-d- "glucopyranosyl" phosphates Carbohydrate Research. 187: 43-66. DOI: 10.1016/0008-6215(89)80055-2  1
1987 Withers SG, Street IP, Bird P, Dolphin DH. 2-Deoxy-2-fluoroglucosides: A novel class of mechanism-based glucosidase inhibitors Journal of the American Chemical Society. 109: 7530-7531.  1
1986 Street IP, Armstrong CR, Withers SG. Hydrogen bonding and specificity. Fluorodeoxy sugars as probes of hydrogen bonding in the glycogen phosphorylase-glucose complex. Biochemistry. 25: 6021-7. PMID 3790503 DOI: 10.1021/bi00368a028  1
1986 Withers SG, Dombroski D, Berven LA, Kilburn DG, Miller RC, J. Warren RA, Gilkes NR. Direct 1H N.M.R. determination of the stereochemical course of hydrolyses catalysed by glucanase components of the cellulase complex Biochemical and Biophysical Research Communications. 139: 487-494. PMID 3094517 DOI: 10.1016/S0006-291X(86)80017-1  1
1986 Withers SG, Street JP, Rettig SJ. The preferred conformation of 2-fluoro-2-deoxy β-D-mannopyranosyl fluoride. An X-ray crystallographic and 2-dimensional proton nuclear magnetic resonance study Canadian Journal of Chemistry. 64: 232-236. DOI: 10.1139/v86-040  1
1985 Withers SG, Madsen NB, Sykes BD. 31P NMR relaxation studies of the activation of the coenzyme phosphate of glycogen phosphorylase. The role of motion of the bound phosphate. Biophysical Journal. 48: 1019-26. PMID 3937556 DOI: 10.1016/S0006-3495(85)83864-9  1
1984 Madsen NB, Withers SG. The catalytic mechanism of phosphorylase; novel role of the coenzyme phosphate. Progress in Clinical and Biological Research. 144: 117-26. PMID 6427781  1
1982 Withers SG, Madsen NB, Sprang SR, Fletterick RJ. Catalytic site of glycogen phosphorylase: structural changes during activation and mechanistic implications. Biochemistry. 21: 5372-82. PMID 7171564  1
1982 Withers SG, Madsen NB, Sykes BD. Covalently activated glycogen phosphorylase: a phosphorus-31 nuclear magnetic resonance and ultracentrifugation analysis. Biochemistry. 21: 6716-22. PMID 6818986  1
1982 Sprang SR, Goldsmith EJ, Fletterick RJ, Withers SG, Madsen NB. Catalytic site of glycogen phosphorylase: structure of the T state and specificity for alpha-D-glucose. Biochemistry. 21: 5364-71. PMID 6816272  1
1982 Withers SG, Shechosky S, Madsen NB. Pyridoxal phosphate is not the acid catalyst in the glycogen phosphorylase catalytic mechanism. Biochemical and Biophysical Research Communications. 108: 322-8. PMID 6816229 DOI: 10.1016/0006-291X(82)91869-1  1
1981 Withers SG, Madsen NB, Sykes BD, Takagi M, Shimomura S, Fukui T. Evidence for direct phosphate-phosphate interaction between pyridoxal phosphate and substrate in the glycogen phosphorylase catalytic mechanism. The Journal of Biological Chemistry. 256: 10759-62. PMID 6793586  1
1981 Withers SG, Madsen NB, Sykes BD. Active form of pyridoxal phosphate in glycogen phosphorylase. Phosphorus-31 nuclear magentic resonance investigation. Biochemistry. 20: 1748-56. PMID 6784759  1
1980 Withers SG, Madsen NB. Nucleotide activation of glycogen phosphorylase b occurs only when the nucleotide phosphate is in a dianionic form. Biochemical and Biophysical Research Communications. 97: 513-9. PMID 6781495  1
1980 Legler G, Sinnott ML, Withers SG. Catalysis by β-glucosidase A3 of Aspergillus wentii Journal of the Chemical Society, Perkin Transactions 2. 1376-1383. DOI: 10.1039/P29800001376  1
1979 Withers SG, Sykes BD, Madsen NB, Kasvinsky PJ. Identical structural changes induced in glycogen phosphorylase by two nonexclusive allosteric inhibitors. Biochemistry. 18: 5342-8. PMID 518839  1
1979 Withers SG, Yang DS, Madsen NB, Fletterick RJ. A systematic approach to isomorphous replacement using a series of simple charged mercurials. Biochemical and Biophysical Research Communications. 86: 1044-50. PMID 435308 DOI: 10.1016/0006-291X(79)90222-5  1
1979 Loeffler RS, Sinnott ML, Sykes BD, Withers SG. Interaction of the lacZ beta-galactosidase of Escherichia coli with some beta-D-galactopyranoside competitive inhibitors. The Biochemical Journal. 177: 145-52. PMID 106843 DOI: 10.1042/bj1770145  1
1978 Sinnott ML, Withers SG. The necessity of magnesium cation for acid assistance aglycone departure in catalysis by Escherichia coli (lacZ) beta-galactosidase. The Biochemical Journal. 175: 539-46. PMID 105722  1
1978 Withers SG, Jullien M, Sinnott ML, Viratelle OM, Yon JM. Dependence upon pH of steady-state parameters for the beta-galactosidase-catalysed hydrolyses of beta-D-galactopyranosyl derivatives of different chemical types. European Journal of Biochemistry / Febs. 87: 249-56. PMID 27358  1
1974 Sinnott ML, Withers SG. The beta-galactosidase-catalysed hydrolyses of beta-d-galactopyranosyl pyridium salts. Rate-limiting generation of an enzyme-bound galactopyranosyl cation in a process dependent only on aglycone acidity. The Biochemical Journal. 143: 751-62. PMID 4462753  1
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