Ichiro Hirao - Publications

Affiliations: 
Institute of Bioengineering & Nanotechnology, Singapore, Singapore 

92 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2021 Alamudi SH, Kimoto M, Hirao I. Uptake mechanisms of cell-internalizing nucleic acid aptamers for applications as pharmacological agents. Rsc Medicinal Chemistry. 12: 1640-1649. PMID 34778766 DOI: 10.1039/d1md00199j  0.721
2020 Kimoto M, Hirao I. Genetic alphabet expansion technology by creating unnatural base pairs. Chemical Society Reviews. PMID 33015699 DOI: 10.1039/d0cs00457j  0.313
2020 Kimoto M, Hirao I. Genetic alphabet expansion technology by creating unnatural base pairs. Chemical Society Reviews. PMID 33015699 DOI: 10.1039/d0cs00457j  0.313
2020 Kimoto M, Soh SHG, Hirao I. Sanger Gap Sequencing for Genetic Alphabet Expansion of DNA. Chembiochem : a European Journal of Chemical Biology. PMID 32202023 DOI: 10.1002/Cbic.202000057  0.347
2019 Kimoto M, Shermane Lim YW, Hirao I. Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA. Nucleic Acids Research. PMID 31392985 DOI: 10.1093/Nar/Gkz688  0.363
2019 Hamashima K, Soong YT, Matsunaga KI, Kimoto M, Hirao I. DNA Sequencing Method Including Unnatural Bases for DNA Aptamer Generation by Genetic Alphabet Expansion. Acs Synthetic Biology. PMID 30995835 DOI: 10.1021/Acssynbio.9B00087  0.372
2018 Yamashige R, Kimoto M, Okumura R, Hirao I. Visual Detection of Amplified DNA by Polymerase Chain Reaction Using a Genetic Alphabet Expansion System. Journal of the American Chemical Society. 140: 14038-14041. PMID 30336010 DOI: 10.1021/Jacs.8B08121  0.352
2018 Hamashima K, Kimoto M, Hirao I. Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology. Current Opinion in Chemical Biology. 46: 108-114. PMID 30059833 DOI: 10.1016/J.Cbpa.2018.07.017  0.413
2017 Kimoto M, Matsunaga KI, Hirao I. Evolving Aptamers with Unnatural Base Pairs. Current Protocols in Chemical Biology. 9: 315-339. PMID 29241296 DOI: 10.1002/Cpch.31  0.401
2017 Kimoto M, Meyer AJ, Hirao I, Ellington AD. Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants. Chemical Communications (Cambridge, England). PMID 29094732 DOI: 10.1039/C7Cc06661A  0.517
2017 Lee KH, Hamashima K, Kimoto M, Hirao I. Genetic alphabet expansion biotechnology by creating unnatural base pairs. Current Opinion in Biotechnology. 51: 8-15. PMID 29049900 DOI: 10.1016/J.Copbio.2017.09.006  0.638
2017 Hirao I, Kimoto M, Lee KH. DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method. Biochimie. PMID 28916151 DOI: 10.1016/J.Biochi.2017.09.007  0.622
2017 Kimoto M, Hirao I. Unique Thermal Stability of Unnatural Hydrophobic Ds Bases in Double-Stranded DNAs. Acs Synthetic Biology. 6: 1944-1951. PMID 28704034 DOI: 10.1021/Acssynbio.7B00165  0.394
2017 Betz K, Kimoto M, Diederichs K, Hirao I, Marx A. Structural Basis for Expansion of the Genetic Alphabet with an Artificial Nucleobase Pair. Angewandte Chemie (International Ed. in English). 56: 12000-12003. PMID 28594080 DOI: 10.1002/Anie.201704190  0.38
2017 Hikida Y, Kimoto M, Hirao I, Yokoyama S. Crystal structure of Deep Vent DNA polymerase. Biochemical and Biophysical Research Communications. 483: 52-57. PMID 28063932 DOI: 10.1016/J.Bbrc.2017.01.007  0.363
2017 Matsunaga KI, Kimoto M, Hirao I. High-Affinity DNA Aptamer Generation Targeting von Willebrand Factor A1-Domain by Genetic Alphabet Expansion for Systematic Evolution of Ligands by Exponential Enrichment Using Two Types of Libraries Composed of Five Different Bases. Journal of the American Chemical Society. 139: 324-334. PMID 27966933 DOI: 10.1021/Jacs.6B10767  0.39
2016 Kimoto M, Nakamura M, Hirao I. Post-ExSELEX stabilization of an unnatural-base DNA aptamer targeting VEGF165 toward pharmaceutical applications. Nucleic Acids Research. PMID 27387284 DOI: 10.1093/Nar/Gkw619  0.39
2016 Okamoto I, Miyatake Y, Kimoto M, Hirao I. High Fidelity, Efficiency and Functionalization of Ds-Px Unnatural Base Pairs in PCR Amplification for a Genetic Alphabet Expansion System. Acs Synthetic Biology. PMID 26814421 DOI: 10.1021/Acssynbio.5B00253  0.406
2016 Kimoto M, Matsunaga K, Hirao I. DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System. Methods in Molecular Biology (Clifton, N.J.). 1380: 47-60. PMID 26552815 DOI: 10.1007/978-1-4939-3197-2_4  0.42
2015 Matsunaga K, Kimoto M, Hanson C, Sanford M, Young HA, Hirao I. Architecture of high-affinity unnatural-base DNA aptamers toward pharmaceutical applications. Scientific Reports. 5: 18478. PMID 26690672 DOI: 10.1038/Srep18478  0.374
2015 Someya T, Ando A, Kimoto M, Hirao I. Site-specific labeling of RNA by combining genetic alphabet expansion transcription and copper-free click chemistry Nucleic Acids Research. 43: 6665-6676. PMID 26130718 DOI: 10.1093/Nar/Gkv638  0.38
2014 Kimoto M, Hirao I. Thermal stability of hydrophobic unnatural base pairs in DNA duplexes Collection of Czechoslovak Chemical Communications. 14: 299-300. DOI: 10.1135/Css201414299  0.335
2014 Kimoto M, Matsunaga K, Hirao I. Generation of high affinity DNA aptamers by the expansion of the genetic alphabet Collection of Czechoslovak Chemical Communications. 14: 97-100. DOI: 10.1135/Css201414097  0.346
2014 Young HA, Hanson C, Sanford M, Matsunaga K, Kimoto M, Hirao I. 208 Cytokine. 70: 78. DOI: 10.1016/J.Cyto.2014.07.215  0.323
2014 Hirao I. Synthetic Genetic Polymers Functioning to Store and Propagate Information by Genetic Alphabet Expansion Reviews in Cell Biology and Molecular Medicine. 1-16. DOI: 10.1002/3527600906.Mcb.20120082  0.383
2013 Kimoto M, Yamashige R, Matsunaga K, Yokoyama S, Hirao I. Generation of high-affinity DNA aptamers using an expanded genetic alphabet. Nature Biotechnology. 31: 453-7. PMID 23563318 DOI: 10.1038/Nbt.2556  0.41
2013 Kimoto M, Matsunaga K, Yamashige R, Hirao I. 3SCA-03 High-affinity DNA aptamer selection by a genetic alphabet expansion PCR system(3SCA Frontier of functional nucleic acids toward elucidation of biological events and nucleic acid medicine,Symposium) Seibutsu Butsuri. 53: S103. DOI: 10.2142/Biophys.53.S103_2  0.363
2013 Attwater J, Tagami S, Kimoto M, Butler K, Kool ET, Wengel J, Herdewijn P, Hirao I, Holliger P. Chemical fidelity of an RNA polymerase ribozyme Chemical Science. 4: 2804-2814. DOI: 10.1039/C3Sc50574J  0.356
2013 Kimoto M, Hikida Y, Hirao I. Site-Specific Functional Labeling of Nucleic Acids by In Vitro Replication and Transcription using Unnatural Base Pair Systems Israel Journal of Chemistry. 53: 450-468. DOI: 10.1002/Ijch.201300013  0.409
2012 Ishizuka T, Kimoto M, Sato A, Hirao I. Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry. Chemical Communications (Cambridge, England). 48: 10835-7. PMID 23032097 DOI: 10.1039/C2Cc36293G  0.372
2012 Kimoto M, Yamashige R, Yokoyama S, Hirao I. PCR amplification and transcription for site-specific labeling of large RNA molecules by a two-unnatural-base-pair system. Journal of Nucleic Acids. 2012: 230943. PMID 22792445 DOI: 10.1155/2012/230943  0.412
2012 Morohashi N, Kimoto M, Sato A, Kawai R, Hirao I. Site-specific incorporation of functional components into RNA by an unnatural base pair transcription system. Molecules (Basel, Switzerland). 17: 2855-76. PMID 22399139 DOI: 10.3390/Molecules17032855  0.408
2012 Hirao I, Kimoto M, Yamashige R. Natural versus artificial creation of base pairs in DNA: origin of nucleobases from the perspectives of unnatural base pair studies. Accounts of Chemical Research. 45: 2055-65. PMID 22263525 DOI: 10.1021/Ar200257X  0.4
2012 Yamashige R, Kimoto M, Takezawa Y, Sato A, Mitsui T, Yokoyama S, Hirao I. Highly specific unnatural base pair systems as a third base pair for PCR amplification. Nucleic Acids Research. 40: 2793-806. PMID 22121213 DOI: 10.1093/Nar/Gkr1068  0.391
2011 Yamashige R, Kimoto M, Mitsui T, Yokoyama S, Hirao I. Monitoring the site-specific incorporation of dual fluorophore-quencher base analogues for target DNA detection by an unnatural base pair system. Organic & Biomolecular Chemistry. 9: 7504-9. PMID 21935564 DOI: 10.1039/C1Ob06118F  0.401
2011 Kimoto M, Cox RS, Hirao I. Unnatural base pair systems for sensing and diagnostic applications. Expert Review of Molecular Diagnostics. 11: 321-31. PMID 21463241 DOI: 10.1586/Erm.11.5  0.369
2010 Kimoto M, Mitsui T, Yamashige R, Sato A, Yokoyama S, Hirao I. A new unnatural base pair system between fluorophore and quencher base analogues for nucleic acid-based imaging technology. Journal of the American Chemical Society. 132: 15418-26. PMID 20939572 DOI: 10.1021/Ja1072383  0.393
2010 Kimoto M, Hirao I. Site-specific incorporation of extra components into RNA by transcription using unnatural base pair systems. Methods in Molecular Biology (Clifton, N.J.). 634: 355-69. PMID 20676996 DOI: 10.1007/978-1-60761-652-8_25  0.397
2010 Hikida Y, Kimoto M, Yokoyama S, Hirao I. Site-specific fluorescent probing of RNA molecules by unnatural base-pair transcription for local structural conformation analysis. Nature Protocols. 5: 1312-23. PMID 20595959 DOI: 10.1038/Nprot.2010.77  0.371
2010 Kimoto M, Mitsui T, Yokoyama S, Hirao I. A unique fluorescent base analogue for the expansion of the genetic alphabet. Journal of the American Chemical Society. 132: 4988-9. PMID 20334374 DOI: 10.1021/Ja100806C  0.421
2009 Kimoto M, Kawai R, Mitsui T, Yokoyama S, Hirao I. An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules. Nucleic Acids Research. 37: e14. PMID 19073696 DOI: 10.1093/Nar/Gkn956  0.401
2008 Fukunaga R, Harada Y, Hirao I, Yokoyama S. Phosphoserine aminoacylation of tRNA bearing an unnatural base anticodon. Biochemical and Biophysical Research Communications. 372: 480-5. PMID 18503748 DOI: 10.1016/J.Bbrc.2008.05.078  0.311
2008 Kimoto M, Mitsui T, Harada Y, Sato A, Yokoyama S, Hirao I. Site-specific incorporation of fluorescent 2-amino-6-(2-thienyl)purine into RNA by transcription using an unnatural base pair system Collection of Czechoslovak Chemical Communications. 10: 355-357. DOI: 10.1135/Css200810355  0.36
2007 Hirao I, Mitsui T, Kimoto M, Yokoyama S. Development of an unnatural base pair for efficient PCR amplification. Nucleic Acids Symposium Series (2004). 9-10. PMID 18029560 DOI: 10.1093/nass/nrm005  0.304
2007 Hirao I, Mitsui T, Kimoto M, Yokoyama S. An efficient unnatural base pair for PCR amplification. Journal of the American Chemical Society. 129: 15549-55. PMID 18027940 DOI: 10.1021/Ja073830M  0.392
2007 Kimoto M, Moriyama K, Yokoyama S, Hirao I. Cytostatic evaluations of nucleoside analogs related to unnatural base pairs for a genetic expansion system. Bioorganic & Medicinal Chemistry Letters. 17: 5582-5. PMID 17804231 DOI: 10.1016/J.Bmcl.2007.07.088  0.331
2007 Kimoto M, Mitsui T, Harada Y, Sato A, Yokoyama S, Hirao I. Fluorescent probing for RNA molecules by an unnatural base-pair system. Nucleic Acids Research. 35: 5360-9. PMID 17693436 DOI: 10.1093/Nar/Gkm508  0.381
2007 Mitsui T, Kimoto M, Kawai R, Yokoyama S, Hirao I. Characterization of fluorescent, unnatural base pairs Tetrahedron. 63: 3528-3537. DOI: 10.1016/J.Tet.2006.11.096  0.354
2006 Hirao I, Kimoto M, Mitsui T, Fujiwara T, Kawai R, Sato A, Harada Y, Yokoyama S. An unnatural base pair system for in vitro replication and transcription. Nucleic Acids Symposium Series (2004). 33-4. PMID 17150803 DOI: 10.1093/nass/nrl017  0.318
2006 Hirao I. Unnatural base pair systems for DNA/RNA-based biotechnology. Current Opinion in Chemical Biology. 10: 622-7. PMID 17035074 DOI: 10.1016/J.Cbpa.2006.09.021  0.393
2006 Hirao I, Kimoto M, Mitsui T, Fujiwara T, Kawai R, Sato A, Harada Y, Yokoyama S. An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA. Nature Methods. 3: 729-35. PMID 16929319 DOI: 10.1038/Nmeth915  0.424
2006 Hirao I. Placing extra components into RNA by specific transcription using unnatural base pair systems. Biotechniques. 40: 711, 713, 715 passim. PMID 16774113 DOI: 10.2144/000112187  0.385
2005 Mitsui T, Kimoto M, Yokoyama S, Hirao I. Fluorescent properties of an unnatural nucleobase, 2-amino-6-(2-thienyl)purine, in DNA and RNA fragments. Nucleic Acids Symposium Series (2004). 285-6. PMID 17150745 DOI: 10.1093/Nass/49.1.285  0.362
2005 Nameki N, Someya T, Okano S, Suemasa R, Kimoto M, Hanawa-Suetsugu K, Terada T, Shirouzu M, Hirao I, Takaku H, Himeno H, Muto A, Kuramitsu S, Yokoyama S, Kawai G. Interaction analysis between tmRNA and SmpB from Thermus thermophilus. Journal of Biochemistry. 138: 729-39. PMID 16428302 DOI: 10.1093/Jb/Mvi180  0.301
2005 Kawai R, Kimoto M, Ikeda S, Mitsui T, Endo M, Yokoyama S, Hirao I. Site-specific fluorescent labeling of RNA molecules by specific transcription using unnatural base pairs. Journal of the American Chemical Society. 127: 17286-95. PMID 16332078 DOI: 10.1021/Ja0542946  0.38
2005 Moriyama K, Kimoto M, Mitsui T, Yokoyama S, Hirao I. Site-specific biotinylation of RNA molecules by transcription using unnatural base pairs. Nucleic Acids Research. 33: e129. PMID 16113238 DOI: 10.1093/Nar/Gni128  0.39
2005 Mitsui T, Kimoto M, Harada Y, Yokoyama S, Hirao I. An efficient unnatural base pair for a base-pair-expanded transcription system. Journal of the American Chemical Society. 127: 8652-8. PMID 15954770 DOI: 10.1021/Ja0425280  0.378
2004 Hirao I, Harada Y, Kimoto M, Mitsui T, Fujiwara T, Yokoyama S. A two-unnatural-base-pair system toward the expansion of the genetic code. Journal of the American Chemical Society. 126: 13298-305. PMID 15479084 DOI: 10.1021/Ja047201D  0.377
2004 Hirao I, Fujiwara T, Kimoto M, Yokoyama S. Unnatural base pairs between 2- and 6-substituted purines and 2-oxo(1H)pyridine for expansion of the genetic alphabet. Bioorganic & Medicinal Chemistry Letters. 14: 4887-90. PMID 15341945 DOI: 10.1016/J.Bmcl.2004.07.038  0.371
2004 Kimoto M, Yokoyama S, Hirao I. A quantitative, non-radioactive single-nucleotide insertion assay for analysis of DNA replication fidelity by using an automated DNA sequencer. Biotechnology Letters. 26: 999-1005. PMID 15269527 DOI: 10.1023/B:Bile.0000030047.32578.82  0.356
2004 Kimoto M, Endo M, Mitsui T, Okuni T, Hirao I, Yokoyama S. Site-specific incorporation of a photo-crosslinking component into RNA by T7 transcription mediated by unnatural base pairs. Chemistry & Biology. 11: 47-55. PMID 15112994 DOI: 10.1016/J.Chembiol.2003.12.016  0.359
2004 Endo M, Mitsui T, Okuni T, Kimoto M, Hirao I, Yokoyama S. Unnatural base pairs mediate the site-specific incorporation of an unnatural hydrophobic component into RNA transcripts. Bioorganic & Medicinal Chemistry Letters. 14: 2593-6. PMID 15109659 DOI: 10.1016/J.Bmcl.2004.02.072  0.377
2004 Hirao I, Harada Y, Nojima T, Osawa Y, Masaki H, Yokoyama S. In vitro selection of RNA aptamers that bind to colicin E3 and structurally resemble the decoding site of 16S ribosomal RNA. Biochemistry. 43: 3214-21. PMID 15023071 DOI: 10.1021/Bi0356146  0.342
2004 Sunami T, Kondo J, Hirao I, Watanabe K, Miura KI, Takénaka A. Structure of d(GCGAAAGC) (hexagonal form): a base-intercalated duplex as a stable structure. Acta Crystallographica. Section D, Biological Crystallography. 60: 90-6. PMID 14684897 DOI: 10.1107/S0907444904001283  0.315
2003 Mitsui T, Kimoto M, Sato A, Yokoyama S, Hirao I. An unnatural hydrophobic base, 4-propynylpyrrole-2-carbaldehyde, as an efficient pairing partner of 9-methylimidazo[(4,5)-b]pyridine. Bioorganic & Medicinal Chemistry Letters. 13: 4515-8. PMID 14643359 DOI: 10.1016/J.Bmcl.2003.09.059  0.33
2003 Mitsui T, Kitamura A, Kimoto M, To T, Sato A, Hirao I, Yokoyama S. An unnatural hydrophobic base pair with shape complementarity between pyrrole-2-carbaldehyde and 9-methylimidazo[(4,5)-b]pyridine. Journal of the American Chemical Society. 125: 5298-307. PMID 12720441 DOI: 10.1021/Ja028806H  0.331
2002 Sunami T, Kondo J, Kobuna T, Hirao I, Watanabe K, Miura K, Takénaka A. Crystal structure of d(GCGAAAGCT) containing a parallel-stranded duplex with homo base pairs and an anti-parallel duplex with Watson-Crick base pairs. Nucleic Acids Research. 30: 5253-60. PMID 12466550 DOI: 10.1093/Nar/Gkf639  0.323
2002 Hirao I, Ohtsuki T, Fujiwara T, Mitsui T, Yokogawa T, Okuni T, Nakayama H, Takio K, Yabuki T, Kigawa T, Kodama K, Yokogawa T, Nishikawa K, Yokoyama S. An unnatural base pair for incorporating amino acid analogs into proteins. Nature Biotechnology. 20: 177-82. PMID 11821864 DOI: 10.1038/Nbt0202-177  0.364
2002 Sunami T, Kondo J, Hirao I, Watanabe K, Miura K, Takenaka A. X-ray structure of D(GCGAAAGCT), parallel-stranded DNA duplex with homo base pairs Acta Crystallographica Section a Foundations of Crystallography. 58: c273-c273. DOI: 10.1107/S0108767302095880  0.322
2001 Fujiwara T, Kimoto M, Sugiyama H, Hirao I, Yokoyama S. Synthesis of 6-(2-Thienyl)purine Nucleoside Derivatives That Form Unnatural Base Pairs with Pyridin-2-one Nucleosides Bioorganic & Medicinal Chemistry Letters. 11: 2221-2223. PMID 11514175 DOI: 10.1016/S0960-894X(01)00415-2  0.352
2001 Ohtsuki T, Kimoto M, Ishikawa M, Mitsui T, Hirao I, Yokoyama S. Unnatural base pairs for specific transcription. Proceedings of the National Academy of Sciences of the United States of America. 98: 4922-5. PMID 11320242 DOI: 10.1073/Pnas.091532698  0.37
2001 Hirao I, Nojima T, Mitsui T, Yokoyama S. Synthesis of DNA Templates Containing the Fifth Base, 2-Amino-6-(dimethylamino)purine, for Specific Transcription Involving Unnatural Base Pairs Chemistry Letters. 30: 914-915. DOI: 10.1246/Cl.2001.914  0.407
2000 Hirao I, Madin K, Endo Y, Yokoyama S, Ellington AD. RNA aptamers that bind to and inhibit the ribosome-inactivating protein, pepocin. Journal of Biological Chemistry. 275: 4943-4948. PMID 10671532 DOI: 10.1074/Jbc.275.7.4943  0.5
2000 Yoshizawa S, Watanabe K, Miura K, Hirao I. The Effect of Hairpin DNA Fragments onEscherichia coliPoly(U)-Dependent Poly(Phe) Synthesis Chemistry Letters. 29: 154-155. DOI: 10.1246/Cl.2000.154  0.365
2000 Hirao I, Ohtsuki T, Mitsui T, Yokoyama S. Dual Specificity of the Pyrimidine Analogue, 4-Methylpyridin-2-one, in DNA Replication Journal of the American Chemical Society. 122: 6118-6119. DOI: 10.1021/Ja993291O  0.349
2000 Ishikawa M, Hirao I, Yokoyama S. Synthesis of 3-(2-deoxy-β-d-ribofuranosyl)pyridin-2-one and 2-amino-6-(N,N-dimethylamino)-9-(2-deoxy-β-d-ribofuranosyl)purine derivatives for an unnatural base pair Tetrahedron Letters. 41: 3931-3934. DOI: 10.1016/S0040-4039(00)00520-7  0.349
1998 Hirao I, Spingola M, Peabody D, Ellington AD. The limits of specificity: an experimental analysis with RNA aptamers to MS2 coat protein variants. Molecular Diversity. 4: 75-89. PMID 10425631 DOI: 10.1023/A:1026401917416  0.472
1998 Rowsell S, Stonehouse NJ, Convery MA, Adams CJ, Ellington AD, Hirao I, Peabody DS, Stockley PG, Phillips SE. Crystal structures of a series of RNA aptamers complexed to the same protein target. Nature Structural Biology. 5: 970-5. PMID 9808042 DOI: 10.1038/2946  0.498
1997 Yoshizawa S, Kawai G, Watanabe K, Miura K, Hirao I. GNA trinucleotide loop sequences producing extraordinarily stable DNA minihairpins. Biochemistry. 36: 4761-7. PMID 9125496 DOI: 10.1021/Bi961738P  0.343
1995 Hirao I, Ellington AD. Re-creating the RNA world Current Biology. 5: 1017-1022. PMID 8542277 DOI: 10.1016/S0960-9822(95)00205-3  0.501
1994 Hirao I, Kawai G, Yoshizawa S, Nishimura Y, Ishido Y, Watanabe K, Miura K. Most compact hairpin-turn structure exerted by a short DNA fragment, d(GCGAAGC) in solution: an extraordinarily stable structure resistant to nucleases and heat. Nucleic Acids Research. 22: 576-82. PMID 8127706 DOI: 10.1093/Nar/22.4.576  0.328
1994 Yoshizawa S, Ueda T, Ishido Y, Miura K, Watanabe K, Hirao I. Nuclease resistance of an extraordinarily thermostable mini-hairpin DNA fragment, d(GCGAAGC) and its application to in vitro protein synthesis. Nucleic Acids Research. 22: 2217-21. PMID 8036147 DOI: 10.1093/Nar/22.12.2217  0.309
1993 Wakiyama M, Hirao I, Kumagai I, Miura K. Effect of tandem repeated AUG codons on translation efficiency of eukaryotic mRNA carrying a short leader sequence. Molecular & General Genetics : Mgg. 238: 59-64. PMID 8479441 DOI: 10.1007/Bf00279531  0.328
1993 Hirao I, Yoshizawa S, Miura K. Stabilization of mRNA in an Escherichia coli cell-free translation system. Febs Letters. 321: 169-72. PMID 8477846 DOI: 10.1016/0014-5793(93)80101-Y  0.334
1992 Hirao I, Nishimura Y, Tagawa Y, Watanabe K, Miura K. Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d(GCFAAAGC)and their effect on DNA sequencing Nucleic Acids Research. 20: 3891-3896. DOI: 10.1093/Nar/20.15.3891  0.375
1991 Hirao I, Yoshizawa S, Miura K. Gel electrophoresis using a fluorescence agent for analysis and purification of non-labeled synthetic DNA fragments. Nucleic Acids Research. 19: 4003. PMID 1861994 DOI: 10.1093/Nar/19.14.4003  0.313
1991 Ishikawa M, Ikebukuro K, Hirao I, Miura K. Synthesis of Fused Oligoribonucleotides with Trideoxyribonucleotide Containing Phosphorothioate to Stabilize Against Nuclease Activity Nucleosides and Nucleotides. 10: 1377-1390. DOI: 10.1080/07328319108047068  0.382
1991 Sakatsume O, Yamaguchi T, Ishikawa M, Hirao I, Miura K, Takaku H. Solid phase synthesis of oligoribonucleotides by the phosphoramidite approach using 2′-O-1-(2-chloroethoxy)ethyl protection Tetrahedron. 47: 8717-8728. DOI: 10.1016/S0040-4020(01)96193-9  0.302
1990 Hirao I, Ishida M, Watanabe K, Miura K. Unique hairpin structures occurring at the replication origin of phage G4 DNA. Biochimica Et Biophysica Acta. 1087: 199-204. PMID 2223881 DOI: 10.1016/0167-4781(90)90205-G  0.357
1990 Hirao I, Okubo M, Ishikawa M, Miura K. Synthesis and Properties of an Initiation Codon Analog Consisting of 2′-O-Methyl Nucleotides Nucleosides and Nucleotides. 9: 1113-1122. DOI: 10.1080/07328319008046067  0.306
1989 Hirao I, Nishimura Y, Naraoka T, Watanabe K, Arata Y, Miura K. Extraordinary stable structure of short single-stranded DNA fragments containing a specific base sequence: d(GCGAAAGC). Nucleic Acids Research. 17: 2223-31. PMID 2704619 DOI: 10.1093/Nar/17.6.2223  0.36
1989 Hirao I, Ishikawa M, Hori H, Watanabe K, Miura K. Synthesis of Nonadeca- and Octadecaribonucleotides Using the Solid-Phase Phosphotriester with Tetrahydropyranyl Groups as the 2′-Hydroxyl-Protecting Group Bulletin of the Chemical Society of Japan. 62: 1995-2001. DOI: 10.1246/Bcsj.62.1995  0.312
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