Robert M. Clegg - Publications

Affiliations: 
Physics University of Illinois, Urbana-Champaign, Urbana-Champaign, IL 
Area:
General Biophysics, Genetics, Molecular Biology, Microbiology Biology

75 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2014 Eichorst JP, Wen Teng K, Clegg RM. Polar plot representation of time-resolved fluorescence. Methods in Molecular Biology (Clifton, N.J.). 1076: 97-112. PMID 24108625 DOI: 10.1007/978-1-62703-649-8_6  0.453
2012 Eichorst JP, Clegg RM, Wang Y. Red-shifted fluorescent proteins monitor enzymatic activity in live HT-1080 cells with fluorescence lifetime imaging microscopy (FLIM). Journal of Microscopy. 248: 77-89. PMID 22971220 DOI: 10.1111/J.1365-2818.2012.03652.X  0.335
2012 Chen YC, Spring BQ, Clegg RM. Fluorescence lifetime imaging comes of age how to do it and how to interpret it. Methods in Molecular Biology (Clifton, N.J.). 875: 1-22. PMID 22573433 DOI: 10.1007/978-1-61779-806-1_1  0.716
2012 Periasamy A, Vogel SS, Clegg RM. FRET 65: a celebration of Förster. Journal of Biomedical Optics. 17: 011001. PMID 22352635 DOI: 10.1117/1.Jbo.17.1.011001  0.332
2012 Ranjan Gartia M, Eichorst JP, Clegg RM, Logan Liu G. Lifetime imaging of radiative and non-radiative fluorescence decays on nanoplasmonic surface Applied Physics Letters. 101. DOI: 10.1063/1.4736575  0.42
2011 Chen YC, Clegg RM. Spectral resolution in conjunction with polar plots improves the accuracy and reliability of FLIM measurements and estimates of FRET efficiency. Journal of Microscopy. 244: 21-37. PMID 21801176 DOI: 10.1111/J.1365-2818.2011.03488.X  0.428
2011 Kolossov VL, Spring BQ, Clegg RM, Henry JJ, Sokolowski A, Kenis PJ, Gaskins HR. Development of a high-dynamic range, GFP-based FRET probe sensitive to oxidative microenvironments. Experimental Biology and Medicine (Maywood, N.J.). 236: 681-91. PMID 21606117 DOI: 10.1258/Ebm.2011.011009  0.704
2011 Matsubara S, Chen YC, Caliandro R, Govindjee, Clegg RM. Photosystem II fluorescence lifetime imaging in avocado leaves: contributions of the lutein-epoxide and violaxanthin cycles to fluorescence quenching. Journal of Photochemistry and Photobiology. B, Biology. 104: 271-84. PMID 21356597 DOI: 10.1016/J.Jphotobiol.2011.01.003  0.436
2011 Gartia MR, Chen Y, Xu Z, Bordain YC, Eichorst J, Mabon JC, Soares JANTD, Clegg RM, Liu GL. Optical characterization of nanopillar black silicon for plasmonic and Solar cell application Proceedings of Spie. 8096. DOI: 10.1117/12.892851  0.326
2009 Chen YC, Clegg RM. Fluorescence lifetime-resolved imaging. Photosynthesis Research. 102: 143-55. PMID 19763875 DOI: 10.1007/S11120-009-9458-7  0.465
2009 Spring BQ, Clegg RM. Image analysis for denoising full-field frequency-domain fluorescence lifetime images. Journal of Microscopy. 235: 221-37. PMID 19659915 DOI: 10.1111/J.1365-2818.2009.03212.X  0.719
2009 Noomnarm U, Clegg RM. Fluorescence lifetimes: fundamentals and interpretations. Photosynthesis Research. 101: 181-94. PMID 19568954 DOI: 10.1007/S11120-009-9457-8  0.419
2009 Chen YC, Spring BQ, Buranachai C, Malachowski G, Clegg RM. What is behind all those lifetimes anyway? Where do we go from here? Progress in Biomedical Optics and Imaging - Proceedings of Spie. 7183. DOI: 10.1117/12.814952  0.788
2008 Vámosi G, Clegg RM. Helix-coil transition of a four-way DNA junction observed by multiple fluorescence parameters. The Journal of Physical Chemistry. B. 112: 13136-48. PMID 18811195 DOI: 10.1021/Jp8034055  0.432
2008 Buranachai C, Kamiyama D, Chiba A, Williams BD, Clegg RM. Rapid frequency-domain FLIM spinning disk confocal microscope: lifetime resolution, image improvement and wavelet analysis. Journal of Fluorescence. 18: 929-42. PMID 18324453 DOI: 10.1007/S10895-008-0332-3  0.73
2008 Kolossov VL, Spring BQ, Sokolowski A, Conour JE, Clegg RM, Kenis PJ, Gaskins HR. Engineering redox-sensitive linkers for genetically encoded FRET-based biosensors. Experimental Biology and Medicine (Maywood, N.J.). 233: 238-48. PMID 18222979 DOI: 10.3181/0707-Rm-192  0.702
2007 Malachowski GC, Clegg RM, Redford GI. Analytic solutions to modelling exponential and harmonic functions using Chebyshev polynomials: fitting frequency-domain lifetime images with photobleaching. Journal of Microscopy. 228: 282-95. PMID 18045323 DOI: 10.1111/J.1365-2818.2007.01846.X  0.777
2007 Spring BQ, Clegg RM. Fluorescence measurements of duplex DNA oligomers under conditions conducive for forming M-DNA (a metal-DNA complex). The Journal of Physical Chemistry. B. 111: 10040-52. PMID 17665942 DOI: 10.1021/Jp0725782  0.683
2007 Ermolenko DN, Spiegel PC, Majumdar ZK, Hickerson RP, Clegg RM, Noller HF. The antibiotic viomycin traps the ribosome in an intermediate state of translocation Nature Structural and Molecular Biology. 14: 493-497. PMID 17515906 DOI: 10.1038/Nsmb1243  0.767
2007 Ermolenko DN, Majumdar ZK, Hickerson RP, Spiegel PC, Clegg RM, Noller HF. Observation of Intersubunit Movement of the Ribosome in Solution Using FRET Journal of Molecular Biology. 370: 530-540. PMID 17512008 DOI: 10.1016/J.Jmb.2007.04.042  0.772
2007 Holub O, Seufferheld MJ, Gohlke C, Heiss GJ, Clegg RM. Fluorescence lifetime imaging microscopy of Chlamydomonas reinhardtii: non-photochemical quenching mutants and the effect of photosynthetic inhibitors on the slow chlorophyll fluorescence transient. Journal of Microscopy. 226: 90-120. PMID 17444940 DOI: 10.1111/J.1365-2818.2007.01763.X  0.441
2006 Clegg RM. A simple derivation of the luminescence anisotropy decay from randomly distributed cylinders rotating about a single axis. Journal of Fluorescence. 16: 761-71. PMID 17096197 DOI: 10.1007/S10895-006-0133-5  0.312
2006 Chandler DE, Majumdar ZK, Heiss GJ, Clegg RM. Ruby crystal for demonstrating time- and frequency-domain methods of fluorescence lifetime measurements. Journal of Fluorescence. 16: 793-807. PMID 17031574 DOI: 10.1007/S10895-006-0123-7  0.796
2005 Redford GI, Majumdar ZK, Sutin JD, Clegg RM. Properties of microfluidic turbulent mixing revealed by fluorescence lifetime imaging. The Journal of Chemical Physics. 123: 224504. PMID 16375486 DOI: 10.1063/1.2132283  0.75
2005 Redford GI, Clegg RM. Polar plot representation for frequency-domain analysis of fluorescence lifetimes. Journal of Fluorescence. 15: 805-15. PMID 16341800 DOI: 10.1007/S10895-005-2990-8  0.78
2005 Hickerson R, Majumdar ZK, Baucom A, Clegg RM, Noller HF. Measurement of internal movements within the 30 S ribosomal subunit using Förster resonance energy transfer Journal of Molecular Biology. 354: 459-472. PMID 16243353 DOI: 10.1016/J.Jmb.2005.09.010  0.773
2005 Majumdar ZK, Hickerson R, Noller HF, Clegg RM. Measurements of internal distance changes of the 30 S ribosome using FRET with multiple donor-acceptor pairs: Quantitative spectroscopic methods Journal of Molecular Biology. 351: 1123-1145. PMID 16055154 DOI: 10.1016/J.Jmb.2005.06.027  0.793
2005 Hanson KM, Clegg RM. Two-photon fluorescence imaging and reactive oxygen species detection within the epidermis. Methods in Molecular Biology (Clifton, N.J.). 289: 413-22. PMID 15502202 DOI: 10.1385/1-59259-830-7:413  0.331
2005 Majumdar ZK, Sutin JDB, Clegg RM. Microfabricated continuous-flow, turbulent, microsecond mixer Review of Scientific Instruments. 76: 1-11. DOI: 10.1063/1.2130332  0.761
2005 Clegg RM, Breusegam S, Barry N, Holub O, Govindjee G, Redford G. How Can One Choose the Best Method for Measuring FRET in a Microscope with My Biological System? Microscopy and Microanalysis. 11. DOI: 10.1017/S1431927605509553  0.753
2005 Majumdar ZK, Hickerson R, Noller HF, Clegg RM. Erratum to “Measurements of Internal Distance Changes of the 30 S Ribosome Using FRET with Multiple Donor–Acceptor Pairs: Quantitative Spectroscopic Methods” [J. Mol. Biol. (2005) 351, 1123–1145] Journal of Molecular Biology. 354: 504. DOI: 10.1016/J.Jmb.2005.10.002  0.761
2004 McKinney SA, Tan E, Wilson TJ, Nahas MK, Déclais AC, Clegg RM, Lilley DM, Ha T. Single-molecule studies of DNA and RNA four-way junctions. Biochemical Society Transactions. 32: 41-5. PMID 14748709 DOI: 10.1042/Bst0320041  0.53
2004 Hohng S, Wilson TJ, Tan E, Clegg RM, Lilley DM, Ha T. Conformational flexibility of four-way junctions in RNA. Journal of Molecular Biology. 336: 69-79. PMID 14741204 DOI: 10.1016/J.Jmb.2003.12.014  0.522
2003 Tan E, Wilson TJ, Nahas MK, Clegg RM, Lilley DM, Ha T. A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate. Proceedings of the National Academy of Sciences of the United States of America. 100: 9308-13. PMID 12883002 DOI: 10.1073/Pnas.1233536100  0.5
2003 Clegg RM, Holub O, Gohlke C. Fluorescence lifetime-resolved imaging: measuring lifetimes in an image. Methods in Enzymology. 360: 509-42. PMID 12622166 DOI: 10.1016/S0076-6879(03)60126-6  0.453
2002 Hanson KM, Clegg RM. Observation and quantification of ultraviolet-induced reactive oxygen species in ex vivo human skin. Photochemistry and Photobiology. 76: 57-63. PMID 12126308 DOI: 10.1562/0031-8655(2002)076<0057:Oaqoui>2.0.Co;2  0.336
2002 Breusegem SY, Clegg RM, Loontiens FG. Base-sequence specificity of Hoechst 33258 and DAPI binding to five (A/T)4 DNA sites with kinetic evidence for more than one high-affinity Hoechst 33258-AATT complex. Journal of Molecular Biology. 315: 1049-61. PMID 11827475 DOI: 10.1006/Jmbi.2001.5301  0.726
2002 Barry NP, Hanson KM, Gratton E, Clegg RM, Behne MJ, Mauro TM. Applications of ultrafast lasers to two-photon fluorescence and lifetime imaging Proceedings of Spie - the International Society For Optical Engineering. 4633: 50-61. DOI: 10.1117/12.461387  0.377
2001 Breusegem SY, Loontiens FG, Regenfuss P, Clegg RM. Kinetics of binding of Hoechst dyes to DNA studied by stopped-flow fluorescence techniques. Methods in Enzymology. 340: 212-33. PMID 11494850 DOI: 10.1016/S0076-6879(01)40424-1  0.73
2001 Breusegem SY, Sadat-Ebrahimi SE, Douglas KT, Bichenkova EV, Clegg RM, Loontiens FG. Experimental precedent for the need to involve the primary hydration layer of DNA in lead drug design. Journal of Medicinal Chemistry. 44: 2503-6. PMID 11472203 DOI: 10.1021/Jm0100943  0.703
2001 Breusegem SY, Sadat-Ebrahimi SE, Douglas KT, Clegg RM, Loontiens FG. Increased stability and lifetime of the complex formed between DNA and meta-phenyl-substituted Hoechst dyes as studied by fluorescence titrations and stopped-flow kinetics. Journal of Molecular Biology. 308: 649-63. PMID 11350167 DOI: 10.1006/Jmbi.2001.4615  0.706
2000 Stühmeier F, Hillisch A, Clegg RM, Diekman S. Fluorescence energy transfer analysis of DNA structures containing several bulges and their interaction with CAP. Journal of Molecular Biology. 302: 1081-100. PMID 11183776 DOI: 10.1006/Jmbi.2000.4089  0.358
2000 Holub O, Seufferheld M, Gohlke C, Govindjee G, Clegg R. Fluorescence Lifetime Imaging (FLI) in Real-Time - a New Technique in Photosynthesis Research Photosynthetica. 38: 581-599. DOI: 10.1023/A:1012465508465  0.452
1998 Vámosi G, Clegg RM. The helix-coil transition of DNA duplexes and hairpins observed by multiple fluorescence parameters. Biochemistry. 37: 14300-16. PMID 9760268 DOI: 10.1021/Bi9727601  0.419
1998 Bassi GS, Murchie AI, Walter F, Clegg RM, Lilley DM. Ion-induced folding of the hammerhead ribozyme: a fluorescence resonance energy transfer study. The Embo Journal. 16: 7481-9. PMID 9405376 DOI: 10.1093/Emboj/16.24.7481  0.319
1997 Stühmeier F, Lilley DM, Clegg RM. Effect of additional unpaired bases on the stability of three-way DNA junctions studied by fluorescence techniques. Biochemistry. 36: 13539-51. PMID 9354622 DOI: 10.1021/Bi970245X  0.406
1997 Stühmeier F, Welch JB, Murchie AI, Lilley DM, Clegg RM. Global structure of three-way DNA junctions with and without additional unpaired bases: a fluorescence resonance energy transfer analysis. Biochemistry. 36: 13530-8. PMID 9354621 DOI: 10.1021/Bi9702445  0.353
1997 Vámosi G, Gohlke C, Clegg RM. Fluorescence characteristics of 5-carboxytetramethylrhodamine linked covalently to the 5' end of oligonucleotides: multiple conformers of single-stranded and double-stranded dye-DNA complexes. Biophysical Journal. 71: 972-94. PMID 8842236 DOI: 10.1016/S0006-3495(96)79300-1  0.368
1997 Schneider PC, Clegg RM. Rapid acquisition, analysis, and display of fluorescence lifetime-resolved images for real-time applications Review of Scientific Instruments. 68: 4107-4119. DOI: 10.1063/1.1148354  0.467
1996 Gadella TWJ, Hoek AAHv, Visser AJWG, Schneider P, Jovin TM, Clegg RM. Frequency-domain fluorescence lifetime imaging microscopy: methodology and examples Scanning. 18: 55-61. DOI: 10.1002/Sca.1996.4950180109  0.396
1995 Clegg RM. Fluorescence resonance energy transfer. Current Opinion in Biotechnology. 6: 103-10. PMID 7534502 DOI: 10.1016/0958-1669(95)80016-6  0.401
1994 Clegg RM, Murchie AI, Lilley DM. The solution structure of the four-way DNA junction at low-salt conditions: a fluorescence resonance energy transfer analysis. Biophysical Journal. 66: 99-109. PMID 8130350 DOI: 10.1016/S0006-3495(94)80765-9  0.376
1994 Gohlke C, Murchie AI, Lilley DM, Clegg RM. Kinking of DNA and RNA helices by bulged nucleotides observed by fluorescence resonance energy transfer. Proceedings of the National Academy of Sciences of the United States of America. 91: 11660-4. PMID 7526401 DOI: 10.1073/Pnas.91.24.11660  0.353
1994 Gadella TWJ, Clegg RM, Jovin TM. Fluorescence lifetime imaging microscopy: pixel-by-pixel analysis of phase-modulation data Bioimaging. 2: 139-159. DOI: 10.1002/1361-6374(199409)2:3<139::Aid-Bio4>3.0.Co;2-T  0.422
1993 Clegg RM, Murchie AI, Zechel A, Lilley DM. Observing the helical geometry of double-stranded DNA in solution by fluorescence resonance energy transfer. Proceedings of the National Academy of Sciences of the United States of America. 90: 2994-8. PMID 8464916 DOI: 10.1073/Pnas.90.7.2994  0.375
1993 Gadella TW, Jovin TM, Clegg RM. Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale Biophysical Chemistry. 48: 221-239. DOI: 10.1016/0301-4622(93)85012-7  0.435
1992 Clegg RM, Murchie AIH, Zechel A, Carlberg C, Diekmann S, Lilley DMJ. Fluorescence Resonance Energy Transfer analysis of the structure of the four-way DNA junction Biochemistry. 31: 4846-4856. PMID 1591245 DOI: 10.1021/Bi00135A016  0.393
1992 Clegg RM. Fluorescence resonance energy transfer and nucleic acids. Methods in Enzymology. 211: 353-88. PMID 1406315 DOI: 10.1016/0076-6879(92)11020-J  0.398
1991 Marriott G, Clegg RM, Arndt-Jovin DJ, Jovin TM. Time resolved imaging microscopy. Phosphorescence and delayed fluorescence imaging. Biophysical Journal. 60: 1374-87. PMID 1723311 DOI: 10.1016/S0006-3495(91)82175-0  0.422
1991 Loontiens FG, McLaughlin LW, Diekmann S, Clegg RM. Binding of Hoechst 33258 and 4',6'-diamidino-2-phenylindole to self-complementary decadeoxynucleotides with modified exocyclic base substituents. Biochemistry. 30: 182-9. PMID 1703013 DOI: 10.1021/Bi00215A027  0.317
1991 Loontiens FG, Regenfuss P, Zechel A, Dumortier L, Clegg RM. Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)], and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities. Biochemistry. 29: 9029-39. PMID 1702995 DOI: 10.1021/Bi00490A021  0.331
1989 Murchie AI, Clegg RM, von Kitzing E, Duckett DR, Diekmann S, Lilley DM. Fluorescence energy transfer shows that the four-way DNA junction is a right-handed cross of antiparallel molecules. Nature. 341: 763-6. PMID 2797209 DOI: 10.1038/341763A0  0.348
1987 Macgregor RB, Clegg RM, Jovin TM. Viscosity dependence of ethidium-DNA intercalation kinetics. Biochemistry. 26: 4008-16. PMID 3651432 DOI: 10.1021/Bi00387A040  0.334
1987 Regenfuss P, Clegg RM. Diffusion-controlled association of a dye, 1-anilinonaphthalene-8-sulfonic acid, to a protein, bovine serum albumin, using a fast-flow microsecond mixer and stopped-flow. Biophysical Chemistry. 26: 83-9. PMID 3593882 DOI: 10.1016/0301-4622(87)80010-8  0.309
1986 Macgregor RB, Clegg RM, Jovin TM. Pressure-jump study of the kinetics of ethidium bromide binding to DNA. Biochemistry. 24: 5503-10. PMID 4074711 DOI: 10.1021/Bi00341A034  0.326
1985 De Boeck H, Macgregor RB, Clegg RM, Sharon N, Loontiens FG. Binding of N-dansylgalactosamine to the lectin from Erythrina cristagalli as followed by stopped-flow and pressure-jump relaxation kinetics. European Journal of Biochemistry. 149: 141-5. PMID 3838937 DOI: 10.1111/J.1432-1033.1985.Tb08904.X  0.347
1985 Loontiens FG, De Boeck H, Clegg RM. Physicochemical aspects of carbohydrate binding to some plant lectins with binding preference forN-acetylgalactosamine and galactose Journal of Biosciences. 8: 425-436. DOI: 10.1007/Bf02703994  0.339
1983 Clegg RM, Loontiens FG, Sharon N, Jovin TM. Dynamic evidence for an extended subsite structure of the ligand combining site on wheat germ agglutinin: temperature-jump relaxation with fluorescence detection. Biochemistry. 22: 4797-804. PMID 6688735 DOI: 10.1021/Bi00289A028  0.33
1983 Loontiens FG, Clegg RM, Van Landschoot A. Some physicochemical aspects of oligosaccharide binding to concanavalin A and wheat germ agglutinin Journal of Biosciences. 5: 105-120. DOI: 10.1007/Bf02702981  0.32
1980 Van Landschoot A, Loontiens FG, Clegg RM, Jovin TM. Binding kinetics of 4-methylumbelliferyl alpha-mannobioside to concanavalin A by fluorescence stopped-flow measurements. European Journal of Biochemistry. 103: 313-21. PMID 6892695 DOI: 10.1111/J.1432-1033.1980.Tb04317.X  0.35
1977 Loontiens FG, Clegg RM, Van Landschoot A, Jovin TM. Binding of 4-methylumbelliferyl alpha-D-mannopyranoside to tetrameric concanavalin A Fluorescence temperature-jump relaxation study. European Journal of Biochemistry. 78: 465-9. PMID 913409 DOI: 10.1111/J.1432-1033.1977.Tb11759.X  0.385
1977 Clegg RM, Loontiens FG, Jovin TM. Binding of 4-methylumbelliferyl alpha-D-mannopyranoside to dimeric concanavalin A: fluorescence temperature-jump relaxation study. Biochemistry. 16: 167-75. PMID 836782 DOI: 10.1021/Bi00621A002  0.395
1977 Loontiens FG, Clegg RM, Jovin TM. Binding of 4-methylumbelliferyl alpha-D-mannopyranoside to tetrameric and unmodified or derivatized dimeric concanavalin A: equilibrium studies. Biochemistry. 16: 159-66. PMID 836781 DOI: 10.1021/Bi00621A001  0.336
1976 Clegg RM, Maxfield BW. Chemical kinetic studies by a new small pressure perturbation method Review of Scientific Instruments. 47: 1383-1393. DOI: 10.1063/1.1134529  0.327
1975 Clegg RM, Elson EL, Maxfield BW. A new technique for optical observation of the kinetics of chemical reactions perturbed by small pressure changes Biopolymers. 14: 883-887. DOI: 10.1002/Bip.1975.360140415  0.457
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