We are testing a new system for linking grants to scientists.
The funding information displayed below comes from the
NIH Research Portfolio Online Reporting Tools and the
NSF Award Database.
The grant data on this page is limited to grants awarded in the United States and is thus partial. It can nonetheless be used to understand how funding patterns influence mentorship networks and vice-versa, which has deep implications on how research is done.
You can help! If you notice any innacuracies, please
sign in and mark grants as correct or incorrect matches.
Sign in to see low-probability grants and correct any errors in linkage between grants and researchers.
High-probability grants
According to our matching algorithm, Change Tan is the likely recipient of the following grants.
Years |
Recipients |
Code |
Title / Keywords |
Matching score |
2008 — 2011 |
Tan, Change |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Generating Conditional Mutations by Random Insertion of An Intein Switch @ University of Missouri-Columbia
Generating Conditional Mutations by Random Insertion of an Intein Switch
Reversible and easy to use, temperature-sensitive (TS) mutations are powerful tools to study the functions of all genes, including essential and pleiotropic ones. However, the rarity of TS alleles and the difficulty of generating and identifying them have limited their use. Working with Drosophila, the PI has developed a novel approach to generate TS mutations efficiently using a conditionally-splicing intein called an intein switch. An intein is an exon that splices itself out of its "host" protein, and an intein switch is an intein in which the splicing activity is temperature-sensitive and thus can be "turned off." The presence of the extra exon in the host protein often inactivates that host protein. The PI's intein switch can be inserted into almost any gene. At the permissive temperature (18 degrees C) it excises itself to generate a wild-type host protein. At the non-permissive temperature (30 degrees C), it fails to splice and remains within the host protein, often leading to the loss of the function of the host protein. This project will generate a multi-purpose construct to facilitate isolation of TS mutations created by insertions of intein switches and will determine the efficacy of using the intein switch to generate conditional mutations. The long-term goal is to make the intein switch a universal tool and to generate a genome-wide collection of TS mutations for systematically assigning function(s) to each gene. This project will develop tools to facilitate generating a genome-wide collection of TS mutations and to perform a pilot screen for providing proof of principle for generating such a collection. This project will broaden diversity by providing support for participation of undergraduate students in research. In addition, the results will be disseminated via a community-outreach program, Saturday Morning Science, to high school students as well as other interested non-scientists.
|
1 |