1984 — 1990 |
Jones, Peter |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Pyi: Mathematical Sciences: Fourier and Complex Analysis |
1 |
1985 |
Jones, Peter A [⬀] |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Azacytidine Induced Differentiation @ University of Southern California
Tissue-specific patterns of 5-methylcytosine in vertebrate DNAs are thought to play significant roles in the control of gene expression in normal and transformed cells. We propose to use the cancer chemotherapeutic agent 5-azacytidine (5-aza-Cr) to perturb these patterns, so that their importance in the stability of cellular phenotypes can be better understood. In particular, we are interested in the relationships between DNA methylation, cellular differentiation and oncogenic transformation. The 5-Aza-Cr will be tested for its ability to induce the formation of epidermal cells from C3H/10T1/2C18 mouse mesenchymal precursors and muscle cells from diploid human fibroblasts. Myogenic lines will be isolated from treated 10T1/2 cells and characterized with respect to chromosomal and 5-methylcytosine contents. Isolated DNA from myogenic lines will be tested for its ability to induce the formation of muscle cells in untreated recipient cells, to test the hypothesis that the emergence of mesenchymal phenotypes is under the control of one or a few regulatory genes. The mechanism of inhibition of DNA methylation by 5-az-Cr and the number of cell divisions required after exposure for new gene expression will also be investigated. Hemimethylated duplex DNA from treated cultures will be used to establish and in vitro methylation assay to isolate and characterize vertebrate DNA methyltransferases. The abilities of diverse chemical carcinogens to inhibit DNA methyltransferases in vitro and in living cells will be investigated. These experiments will determine whether chemical carcinogens can induce heritable changes in cellular phenotype by altering DNA methylation patterns.
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0.928 |
1985 — 1987 |
Jones, Peter A [⬀] |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Basement Membrane Degradation by Tumor Cells @ University of Southern California
This research seeks to define the biochemistry and cell biology of endothelial cell basement membrane degradation by malignant cells. Basement membranes elaborated by cultured bovine endothelial cells and labeled with radioactive precursors in specific components will be prepared using novel techniques developed in this laboratory. The subendothelial matrix has been characterized biochemically by sequential enzyme hydrolysis, gel electrophoresis, electron microscopy, and immunofluorescence. The degradation of basement membrane by human tumor cell lines has been investigated in detail and the role of tumor proteases, especially the tumor cell plasminogen activator, defined. Human fibrosarcoma cells are capable of the complete degradation of the subendothelial matrix after removal of the endothelial cells. We also have found that living endothelial cells secrete a factor which inhibits the degradation of the membrane by tumor cells. This regulation of tumor cell aggressive behavior by a normal cell type could have major importance in the metastatic process and we are characterizing it in more detail. Subendothelial matrices also are being used for the routine culture of primary explants of human pediatric tumors. The subendothelial matrix also stimulates the differentiation of normal cells cultured upon it and we are determining which factors within the subendothelium play important roles in this stimulation. The subendothelial matrix, therefore, can be degraded by invasive cells and can modulate the differentiation of certain other cell types, and endothelial cells themselves inhibit protease production by malignant cells. All of these properties may play key roles in the establishment of secondary tumors during metastasis. (A)
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0.928 |
1986 — 1988 |
Jones, Peter A [⬀] |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
5-Azacytidine Induced Differentiation @ University of Southern California
The long term goal of this project is to utilize ananucleoside analogs of cytidine to understand the role of DNA methylation in cell differentiation. We have previously shown that 5-azacytidine (5-aza-CR) and 5-azadeoxycytidine (5-aza-CdR) induce profound changes in the differentiated state of mouse C3H 10T1/2 C18 cells and have attributed these changes to the abilities of the analogs to perturb DNA methylation. We now want to undertake more detailed studies on the mechanisms of 5-azanucleoside action and to use clonal derivative of 10T1/2 cells treated with these agents to understand aspects of de novo methylation and the molecular control of cell differentiation. The mechanism of cytotoxicity by 5-aza-CdR will be investigated in a series of clonal derivatives derived after multiple treatments of 10T1/2 cells with the drug. These cells are markedly resistant to the cytotoxic activity of 5-aza-CdR and will be defined with respect to gene amplification, 5-aza-CdR incorporation and the interaction of incorporated 5-azacytosine with nuclear proteins. The cells have markedly reduced levels of 5-methylcytosine (5mC) and undergo consideragle de novo methylation when dividing in the absence of further drug treatment. We will define pathways for the establishment of new methylation patterns within the cells at the level of specific gene sequences. we will also attempt to experimentally raise DNA 5mC levels within cells by treatment with inhibitors of DNA synthesis so that DNA hypermethylation can be modulated and genes may be extinguished within cells. A major part of the proposal is devoted to themolecular and cellular characterization of myogenic derivatives derived from 10T1/2 cells by 5-aza-CR treatment. We will attempt to isolate genes specific for the commitment phase of myogenic differentiation within these cells and define their methylation status and control. The hypothesis being tested here is that 5-aza-CR induces the undermethylation of one or a small number of genes whose subsequent expression defines the myogenic phenotype. Overall, these studies will increase our knowledge of the mechanisms of 5-aza-CdR action, define mechanisms for de novo methylation within cells and characterize regulatory genes in differentiating mouse cells.
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0.928 |
1986 — 1990 |
Jones, Peter |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Mathematical Sciences: Hardy Spaces and Euclidean Harmonic Analysis |
1 |
1987 — 1998 |
Jones, Peter A [⬀] |
T32Activity Code Description: To enable institutions to make National Research Service Awards to individuals selected by them for predoctoral and postdoctoral research training in specified shortage areas. |
Program in Molecular Oncology @ University of Southern California |
0.928 |
1988 — 1989 |
Jones, Peter A [⬀] |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Molecular Analysis of Human Bladder Cancer @ University of Southern California
The major emphasis of this proposal is to define the role of oncogenes, growth factors and DNA methylation in the growth and mutation of normal human urothelium and to determine how gene regulation and response to growth factors are altered during oncogenic transformation, tumor growth and metastasis. The proposal relies on the use of a serum-free medium we have developed for the growth of normal and neoplastic urothelial cells. It will be used to define the role of putative endogenous and exogenous growth factors in the division and maturation of normal urothelial cells. Studies to identify appropriate markers for maturation of urothelium in vitro will be continued and the effects of different growth conditions on the expression of the differentiated phenotype will be studied. Co-cultured experiments will investigate interactions between epithelial cells and bladder mesenchymal cells cultured from the same tissue source to determine the effect of stromal-epithelial interactions in normal maturation and growth. The next major aim is to determine how growth factor requirements and cell-cell interactions are altered in cell lines derived from papillary tumors and invasive bladder cancer cells so we may determine how these properties are modified by neoplasia, invasion and metastasis. The tissue culture studies will be complemented by a molecular analysis of genes thought to be important in epithelial differentiation and growth including the EGF receptor and the c- Ha-ras genes. The mechanisms of extracellular matrix breakdown by invasive transitional cell carcinomas will be investigated and we will continue the development of a nude mouse model we have described which allows for human bladder tumor metastasis. This model will be used to isolate metastatic cells and also to determine whether 5-azadeoxycytidine treatment can alter bladder tumor cell behaviour by alterations in DNA methylation patterns. As a complement to this study, we are isolating genes activated in osteogenic sarcoma cells by 5-azadeoxycytidine treatment and which may play a role in the tumorigenicity of these cells. Finally, we will conduct an RFLP analysis of human transitional cell carcinomas to determine the potential relevance of hemi and/or homozygosity for chromosomes 9 and 11 to bladder cancer.
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0.928 |
1989 — 1993 |
Jones, Peter Beals, Richard [⬀] Coifman, Ronald (co-PI) [⬀] |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Mathematical Sciences: Analysis: Partial Differential Equations, Fourier & Complex Analysis and Group Project in Mathematical Wavelets
Modern techniques of mathematical analysis will be applied to fundamental problems in differential equations, complex analysis, wavelet theory, and dynamical systems in this group research project. Work on inverse scattering continues efforts to study higher dimensional problems by means of a new approach derived from methods of several complex variables: the d-bar method. Although this approach has had considerable formal success in two space dimensions and some success in higher dimensions, very few problems have been analyzed rigorously. In particular, work will be done on problems with large potentials or with general singular scattering data. Recent analyses of the d-bar-Neumann problem associated to domains in a complex manifold have shown that the corresponding trace of the heat kernel (for the heat equation) has a small time asymptotic, expansion whose coefficients are integrals of local geometric invariants, including the logarithmic terms. Very little is known about these invariants beyond some general results about their form and degree of homogeneity. Work will continue in efforts to elicit more concrete information. New proofs of the boundedness of the Cauchy integral and other singular integral using special bases has opened up areas of exploration both in operator theory and numerical analysis. Using basic Littlewood-Paley theory, one can translate the T(1) theorem of David and Journe into an effective numerical tool. This powerful result establishes the boundedness of a singular operator solely through its action on the constant functions. Work will be done in representing integral operators in wavelet bases as matrices which decay rapidly off the diagonal. These techniques can be shown to produce considerable reduction in complexity in the discrete approximation to the operators. A wide range of applicability of these ideas to signal and image processing is expected as a consequence of this research. Other work will focus on relationships between the Cauchy integral, analytic capacity and rectifiable sets. Work directed at characterizing rectifiable curves has led to new insights into the traveling salesman problem. Further applications will be considered relative to the problem of estimating the integral of the derivative of a conformal mapping and on determining the relationship between analytic capacity and Favard length of compact planar sets. Although they are not equivalent quantities, it is likely that they do agree on sets of finite one-dimensional Hausdorff measure. Work will be done to resolve this issue. //
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1 |
1989 — 2000 |
Jones, Peter A [⬀] |
R35Activity Code Description: To provide long term support to an experienced investigator with an outstanding record of research productivity. This support is intended to encourage investigators to embark on long-term projects of unusual potential. |
Dna Methylation in Development and Cancer @ University of Southern California
The focus of this proposal is to define the role of DNA methylation in development and cancer. We will investigate the influence of DNA methylation on the expression of determination and transforming genes and study interactions between them, determine how methylation patterns evolve during tumor progression and test the hypothesis that DNA methylation can skew the genetics of some human cancers. Cells of the mouse embryo cell line 10T1/2 primed to differentiate with 5-azacytidine, will be used to investigate hierarchies of gene control in mesenchymal determination and differentiation. I am particularly interested in defining the role of methylcytosine in this process. The influence of cellular determination on oncogenesis and the interplay between transforming and determination genes will ge investigated in the same system. The potential for DNA methylation to silence transforming genes will be examined by the molecular cloning and characterization of a novel oncogene in human osteogenic sarcoma cells treated with 5- azadeoxycytidine. Changes in methylation levels and patterns during tumor progression will be tracked in fresh samples of human bladder tumors. The possibility that allele specific methylation of human genes can skew the genetics of human cancer, due to the inherent mutagenic activity of 5-methylcytosine, will be examined in human retinoblastoma and Wilms' tumor.
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0.928 |
1990 |
Jones, Peter A [⬀] |
C06Activity Code Description: To provide matching Federal funds, up to 75%, for construction or major remodeling, to create new research facilities. In addition to basic research laboratories this may include, under certain circumstances, animal facilities and/or limited clinical facilities where they are an integral part of an overall research effort. |
Cancer Construction Grant @ University of Southern California |
0.928 |
1991 — 1992 |
Jones, Peter A [⬀] |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Gene Regulation in Embryonic Cell Differentiation @ University of Southern California
teratoma; genetic regulation; cell differentiation; histocompatibility antigens; nucleic acid probes; heterophile antigens; genetic library; molecular cloning; gene expression; genetic transcription; major histocompatibility complex; neoplasm /cancer genetics; tissue /cell culture; laboratory mouse;
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0.928 |
1992 — 1996 |
Jones, Peter Beals, Richard [⬀] Coifman, Ronald (co-PI) [⬀] |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Mathematical Sciences: Partial Differential Equations & Analysis
This award supports the work of three researchers investigating several areas of broad mathematical interest and importance. Some work will be conducted jointly while other will be addressed by single investigators. One can divide the studies into three main themes: nonlinear partial differential equations, wavelet analysis and complex function theory. In the first, efforts will be made to explore how the one-dimensional quantum version of the inverse scattering methods which replace the classical action-angle variables can be applied to understanding the corresponding higher dimensional systems. Work is also progressing on the investigation of inverse geophysical problems involving surface wave phenomena. Progress on model scalar cases suggests that analysis of the full system of equations can be obtained. Studies of adapted wave form analysis, a collection of FFT- like adapted transform algorithms, continues to discover and refine new orthonormal bases which decompose functions and operators into almost diagonal form. The interplay between these theoretical advances and the application to numerical signal processing has led to highly successful decomposition techniques using entropy based stopping time searches. The applications lead, in turn, to a host of new questions in harmonic analysis. One of these will be that of creating a new concept of theoretical dimension of a function designed to measure the number of parameters necessary to describe the function with wave forms taken from a given library. Studies on the fine structure of harmonic measure also continue. Here the goals are to find the asymptotic behavior of harmonic measure in a variety of settings. Work will also be done estimating the length of level lines of the Green's function on bounded simply connected domains. It is believed that level line length varies with the fourth root of the value of constancy. Partial differential equations form the backbone of mathematical modeling in the physical sciences. Phenomena which involve continuous change such as that seen in motion, materials and energy are known to obey certain general laws which are expressible in terms of the interactions and relationships between partial derivatives. The key role of mathematics is not to state the relationships, but rather, to extract qualitative and quantitative meaning from them and validate methods for expressing solutions. Harmonic analysis focuses on the decomposition of functions into component parts which best reflect their oscillatory characteristics, while complex function theory seeks to describe the geometric and analytic properties of differentiable functions of a complex variable and their generalizations to higher dimensions.
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1 |
1993 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Usc Comprehensive Cancer Center (Core) Support @ University of Southern California
The Kenneth Norris Jr. comprehensive Cancer Center at the University of Southern California has been in existence since mid-1971 and continuously supported by a Cancer Center (Core) Support Grant since 1973. In the intervening years, the Center has developed to a major regional resource for cancer research, treatment, prevention and education. Center facilities have been built and developed with support from the NCI, the University, and, in its early days, the County of Los Angeles. Research is organized into six program areas, each headed by two senior investigators: Gene Regulation; Tumor Biology; lmmunobiology, Pathogenesis and Treatment; Developmental Therapeutics; Cancer Etiology; and Cancer Prevention Research and Education. In this application we are requesting the following: 1. partial salary support for the Center's senior leadership who are responsible for planning and overseeing our research efforts; 2. partial salary support for selected staff investigators who qualify according to NCI guidelines; 3. support for planning and evaluation to keep the Center at the forefront of cancer research; 4. support for the administrative infrastructure of the Center; 5. partial support for the Center's shared resources and the development of one new facility; 6. and support for developmental funds to help us recruit new investigators in the areas we have targeted for expansion. Continued funding from the Cancer Center (Core) Support Grant program will allow us build on and expand our strengths and to develop initiatives in promising areas of cancer research, treatment, prevention and education.
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0.928 |
1994 — 1995 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Usc Comprehensive Can Ctr (Core) Support @ University of Southern California
The Kenneth Norris Jr. comprehensive Cancer Center at the University of Southern California has been in existence since mid-1971 and continuously supported by a Cancer Center (Core) Support Grant since 1973. In the intervening years, the Center has developed to a major regional resource for cancer research, treatment, prevention and education. Center facilities have been built and developed with support from the NCI, the University, and, in its early days, the County of Los Angeles. Research is organized into six program areas, each headed by two senior investigators: Gene Regulation; Tumor Biology; lmmunobiology, Pathogenesis and Treatment; Developmental Therapeutics; Cancer Etiology; and Cancer Prevention Research and Education. In this application we are requesting the following: 1. partial salary support for the Center's senior leadership who are responsible for planning and overseeing our research efforts; 2. partial salary support for selected staff investigators who qualify according to NCI guidelines; 3. support for planning and evaluation to keep the Center at the forefront of cancer research; 4. support for the administrative infrastructure of the Center; 5. partial support for the Center's shared resources and the development of one new facility; 6. and support for developmental funds to help us recruit new investigators in the areas we have targeted for expansion. Continued funding from the Cancer Center (Core) Support Grant program will allow us build on and expand our strengths and to develop initiatives in promising areas of cancer research, treatment, prevention and education.
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0.928 |
1994 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Comprehensive Cancer Center (Core) Support @ University of Southern California |
0.928 |
1995 — 1999 |
Jones, Peter Beals, Richard [⬀] Coifman, Ronald (co-PI) [⬀] |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Mathematical Sciences: Partial Differential Equations and Analysis
DMS-9423746 PI: R. Beals, R. R. Coifman, P. W. Jones Abstract The principal investigators will continuing their work in a number of related areas of analysis: harmonic analysis, nonlinear Fourier analysis, and partial differential equations, with applications to complex analysis, physical problems, and numerical work. Beals and collaborators will make more use their methods from Hamiltonian mechanics to investigate fundamental solutions for subelliptic laplacians. Beals will also continue joint work on the quantized three-wave interaction and on geophysical inverse problems. Coifman and Meyer will continue to investigate nonlinear Fourier analysis and applications to homogenization and nonlinear dependence. Coifman will also continue to develop adapted wave form analysis to obtain geometric and numerical understanding of oscillatory operators. Jones will continue his work on the fine properties of harmonic measure and level lines of Green's function, as well as work on singular integrals and d-bar problems related to corona problems and analytic capacity. Jones also plans further investigations in quasiconformal mappings and problems relating Sobolev spaces and geometry of sets. Analysis is the area of mathematics that has grown directly from the invention of calculus and differential equations. It provides a framework for attacking problems that range from the purely mathematical and geometrical to the most concrete scientific and technical questions. In turn, these problems have provided the motivation for the continued refinement of the subject and, not incidentally, its continued utility. Thus there is no precise dividing line between pure theory and potential or actual application. For example, in little more than a decade sophisticated techniques of pure harmonic analysis have emerged in practice as wavelet analysis and have enormously extended the possibilities for analysis and storage of digital data. The sub jects to be investigated in this proposal reflect the full range of analysis as well as the actual and potential interplay between "pure" and "applied." Some have a history of a century or more and come from classical analysis and geometry, others have a history of decades and are motivated by physics and geophysics, others have arisen in the last decade and are connected with signal processing and the concrete use of computers in science and technology.
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1 |
1996 — 2010 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Usc/Norris Comprehensive Cancer Center (Core) Support @ University of Southern California
DESCRIPTION (provided by applicant): The USC/Norris Comprehensive Cancer Center at the University of Southern California was founded in 1971 and has been continuously supported by a Cancer Center Core Support Grant since 1973. It has developed into a major regional and national resource for cancer research, treatment, prevention and education. Center facilities have been built and developed with support from the NCI, the University and the Kenneth Morris Jr. Cancer Hospital, which is an integral part of the Cancer Center. The Center underwent a major expansion with the addition of the Norman Topping Tower in 1996 and is currently building a third facility, the Harlyne Morris Translational Research Tower. The Center's 191 members are organized into five thematic and five translational research programs, including a "bridge" program in developmental therapeutics. The thematic programs are headed by senior investigators in the fields of molecular genetics, epigenetics and regulation, tumor microenvironment, cancer epidemiology and cancer control research. The translational programs, which provide focus to our multidisciplinary research efforts, are in developmental therapeutics, genitourinary cancers, gastrointestinal cancers, women's cancers and hematologic and viral-associated malignancies. This application is for: 1) partial salary support for the Center's senior leadership, who are responsible for planning and overseeing our research efforts;2) support for program planning and evaluation to keep the Center at the forefront of cancer research;3) support for the administrative infrastructure of the Center;4) partial support for the Center's 13 shared resources which facilitate the conduct of our peer-reviewed research;and 5) support for developmental funds to help us recruit new investigators in areas we have targeted for expansion. Continued funding from the Cancer Center Core Support Grant will allow us to build on our strengths in basic and population-based research and to translate our underlying expertise into strong, peer-reviewed funded research programs focused on cancers of major consequence in this country. Cancer Center members currently hold grants totaling $91 million in direct costs, with $40 million of that coming from NCI (all exclusive of the CCSG).
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0.928 |
1999 — 2016 |
Jones, Peter A [⬀] |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. R37Activity Code Description: To provide long-term grant support to investigators whose research competence and productivity are distinctly superior and who are highly likely to continue to perform in an outstanding manner. Investigators may not apply for a MERIT award. Program staff and/or members of the cognizant National Advisory Council/Board will identify candidates for the MERIT award during the course of review of competing research grant applications prepared and submitted in accordance with regular PHS requirements. |
Mechanisms of De Novo Methylation in Cancer @ University of Southern California
Carcinogenesis is characterized by the accumulation of multiple genetic and epigenetic changes in cancer cells. De novo methylation of CpG islands which are generally not methylated on autosomal genes in normal cells, is one of the most common genomic alterations in human cancers and occurs in parallel with a genome wide demethylation during carcinogenesis. The aberrant methylation of CpG islands, if it occurs in promoter regions, contributes to the inactivation of tumor suppressor, growth regulatory and DNA repair genes. Epigenetic modification of CpG islands is now considered to be a significant contributor to gene inactivation during carcinogenesis. The objectives of this proposal are to determine the underlying molecular mechanisms for this abnormal de novo methylation using new quantitative methods developed in this laboratory and to take advantage of new discoveries of DNA methyltransferase enzymes, which may play important roles in this process. This will be achieved by the accomplishment of four specific aims in which we will first investigate the relationship between transcription and de novo methylation and test the hypothesis that transcription through a CpG island may facilitate de novo methylation. To probe further the relationship between transcription and de novo methylation, we will investigate the chromatin structure of CpG islands within promoters and downstream of promoters with specific emphasis on the state of histone acetylation. We will also characterize the potential roles of newly described putative de novo methyltransferases in human cancer, and test the effects of overexpression of these enzymes and the effects of nullifying their activities by antisense approaches. The successful achievements of these specific aims will allow us to gain a better understanding of the mechanisms of de novo methylation as they pertain to transcription, influences on chromatin structure and the role of the new DNA methyltransferase enzymes in human carcinogenesis.
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0.928 |
1999 — 2003 |
Jones, Peter Coifman, Ronald (co-PI) [⬀] |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Application of Harmonic Analysis and Geometry of Sets
Proposal: DMS-9971311 Principal Investigators: Peter W. Jones, Ronald R. Coifman
Abstract: Professors Coifman and Jones will continue their work on the analysis and geometry of sets in Euclidean space. The research is centered on methods to classify sets or functions and to provide efficient descriptions of these objects. On the level of sets, this involves descriptions of, for example, the Julia set associated with a rational mapping or the limit set of a Kleinian group in terms of its Hausdorff dimension or related geometric concepts. Another example relates to potential theoretic aspects of sets, including algorithms that allow for fast computations. For functions, the relevant objective is to find short descriptions with prescribed error. A common aspect of the analysis of both sets and functions is the search for methods of reducing dimensionality (with prescribed error) so as to bring the problem into a regime where more classical methods apply.
The problems on which Jones and Coifman intend to work are those that now confront mathematicians who are forced to deal with massive quantities of data. While the modern computer has been very useful in treating many computational problems, severe limitations occur when the amount of data becomes too large. Instead of treating the full set of data, one therefore seeks to reduce significantly the amount of information being handled, at the same time ensuring that only small errors occur in the process. After such a reduction has been made, more classical methods can then be used to analyse the reduced data. Harmonic analysis is a field that has been developed over the past century to accomplish precisely this kind of reduction, albeit for problems in the realm of pure mathematics. The projected research centers on exploiting these older methods and developing new ones in order to acquire efficient tools for treating a wide array of concrete data sets. One example of how useful this technique might be in a practical, everyday situation arises in the telephone industry, where it would be highly desirable to send only a small percentage of the sounds produced by one person, yet still have the message understood by the person listening at the other end of the line. The development of an effective method for compressing data in this instance would allow a large increase in the number of calls that could be sent on a single telephone line.
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1 |
2000 — 2004 |
Kannan, Ravindran (co-PI) [⬀] Coifman, Ronald (co-PI) [⬀] Howe, Roger (co-PI) [⬀] Jones, Peter Mandelbrot, Benoit |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Yale Vigre Program in Mathematics and Applied Mathematics
Abstract:
The Yale VIGRE program will build on the strong base of an existing inter-disciplinary and substantially vertically integrated program by creating vertically integrated project groups (VPGs) to carry out defined research and development agendas in pure and applied mathematics, curriculum development, education and outreach. VIGRE at Yale will support undergraduates, graduate students and post-docs working together, with the guidance of faculty, for a diverse and flexible set of goals. It will provide undergraduates with a new range of opportunities to encounter mathematics in a more immediate and vital way than traditional coursework. It will broaden and deepen graduate training, and provide for shortened length of study. It will provide students and postdocs with opportunities to deepen their understanding of and expertise in teaching. It will develop and disseminate curricular materials for courses embodying new mathematical ideas, and new approaches to traditional topics.
This program is being jointly funded by the Division of Mathematical Sciences and the Office of Multidisciplinary Activities from the Directorate of Mathematical and Physical Sciences.
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1 |
2000 — 2014 |
Jones, Peter A (co-PI) [⬀] Liang, Gangning None |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
De Novo Dna Methylation in Bladder Cancer @ University of Southern California
DESCRIPTION (provided by applicant): The objectives of this grant, which has been funded for almost 30 years, are to understand the genetic and epigenetic basis of human bladder cancer. We have defined the existence of two molecular pathways for the genesis of bladder cancer (UC) in which superficial (Ta/T1) tumors, which frequently recur, are distinct from more aggressive tumors at the molecular level. We have also shown profound epigenetic alterations which occur during bladder carcinogenesis and want to continue our studies by using more global approaches to define key genes which may play a role in this prevalent but understudied disease. In the next five year period of the grant, we will use a series of eight hypermethylation markers to complete the examination of DNA in urine sediments obtained from individuals with low grade tumors to determine whether we can detect the frequent recurrences of these tumors. We shall also complete the analysis of DNA from healthy individuals of different ages to determine whether age-related changes in DNA methylation can be detected in urine sediments. Secondly, using high-throughput approaches on the Illumina platform, we have observed a hypomethylation phenotype not seen in the apparently normal urothelium but particularly prevalent in superficial tumors and less frequently in more invasive tumors. This hypomethylation is seen in the vicinity of transcription start sites and might play a role in ectopic gene activation in tumors. In Specific Aims 3 and 4 we will determine the functional significance of these changes by analyzing directly whether methylation of non- CpG island regions which constitute the bulk of the hypomethylation phenotype might be involved in gene activation and have chromatin properties associated with active genes. Finally, in Specific Aim 5 we will take advantage of ongoing clinical trials in which patients with myelodysplastic syndrome are being treated with the hypomethylating drug 5-azacytidine (5-aza-CR). Since we can easily and non-invasively obtain urine sediments from these patients, we can directly test the hypothesis that systemic administration of hypomethylating drugs leads to demethylation of bladder urothelial cells which may have implications in the future for treatment of this disease.
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0.928 |
2002 — 2006 |
Jones, Peter A [⬀] |
P01Activity Code Description: For the support of a broadly based, multidisciplinary, often long-term research program which has a specific major objective or a basic theme. A program project generally involves the organized efforts of relatively large groups, members of which are conducting research projects designed to elucidate the various aspects or components of this objective. Each research project is usually under the leadership of an established investigator. The grant can provide support for certain basic resources used by these groups in the program, including clinical components, the sharing of which facilitates the total research effort. A program project is directed toward a range of problems having a central research focus, in contrast to the usually narrower thrust of the traditional research project. Each project supported through this mechanism should contribute or be directly related to the common theme of the total research effort. These scientifically meritorious projects should demonstrate an essential element of unity and interdependence, i.e., a system of research activities and projects directed toward a well-defined research program goal. |
Molecular Mechanisms of Human Bladder Carcinogenesis @ University of Southern California
Revised Abstract: The major goals of this Program Project Grant are to utilize two unique and complimentary hospital and population-based bladder cancer databases to further understand the etiology, diagnosis and prognosis of transitional cell carcinoma of the bladder. Project 1 will determine the role of de novo methylation of regulatory genes in the initiation and progression of human bladder cancer and will examine the effects of cigarette smoking and use of non-steroidal anti-inflammatory drugs (NSAIDs) use on the patterns of abnormal methylation. Project 2 will determine the role of COX-2 and DNA Methyltransferase expression in primary tumors and relate the expression levels to exposure levels of cigarette smoking and NSAID use. Project 3 will determine how the altered expression of cell-cycle regulatory genes plays a role in the genesis and prognosis of bladder cancer and will examine the expression of proteins encoded by genes studied in Projects 1 and 2, with the long-term goal of developing better techniques for predicting and for establishing better, more rational treatment. The Program Project will cover a wide range of aspects of bladder cancer, extending from cellular and molecular events during genesis of bladder cancer, through epidemiology, to clinically relevant translational issues such as chemoprevention, prediction of risk for recurrence and treatment response. The achievement of these goals will be assisted by three Core Facilities including an Administrative Core, a Biostatistics and Bladder Cancer Database Core and a Pathology Core. The goals of the Program Project Grant will be furthered by the close juxtapositioning of the research labs and the offices of the principal investigators involved in most of the experiments to be performed. The participating investigators have a considerable history of collaboration in the areas of translational research in the field of bladder cancer and these interactions will be formalized and enhanced by this Program Project Grant.
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0.928 |
2002 — 2005 |
Jones, Peter |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Collaborative Research: a Focused Research Group On Multiscale Geometric Analysis: Theory, Tools, Applications
Proposal IDs: DMS-0140698, DMS-0140540, DMS-0140587, DMS-0140623 PIs: Donoho, Candes, Huo and Jones TITLE: A Focused Research Group on Multiscale Geometric Analysis--Theory, Tools, Applications
Abstract
An interdisciplinary team, bridging Harmonic Analysis, Statistics, Image Analysis and Astronomy, proposes a united effort to exploit and extend recent breakthroughs in computational harmonic analysis. The team will consolidate several scattered advances into a unified body of theory and methods to be called Multiscale Geometric Analysis, which can probe the fine structure of 2-, 3- and higher- dimensional functions and point clouds, able to isolate and manipulate intermediate-dimensional phenomena. Simple examples include edges in 2-d images, filaments and sheets in 3-d point catalogs. Characterizing such intermediate-dimensional phenomena is essential for fundamental progress in a wide range of problem areas (including 2-d and 3-d imaging) where traditional multiscale methods have now run their course. Our united effort has three main outcomes. 1. Theory. Coherent, comprehensive knowledge, showing what can and cannot be accomplished with computational harmonic analysis. 2. Tools. A wide range of practical MGA algorithms and a unified, publicly available software environment - BeamLab - deploying them. 3. Applications. Our main initial focus will be on the analysis of 3-d point catalogs in astronomy, such as those coming on-line soon from the Sloan Digital Sky Survey. We know a priori that the data contain filaments and sheets, embedded in a scattered background, and MGA provides a decisive set of tools to resolve the structural properties of such catalogs, far more sensitive and more comprehensible than any tools currently available for such analysis.
Many new kinds of massive databases are being created in our era's revolutionary transition to a data-rich society. Many of these databases contain geometric structures such as surfaces, and filaments, albeit in a sometimes hidden manner. Databases of this sort can include galaxy catalogs in astronomy -- in which the filaments and sheets are predicted by various theories of universe formation -- 3-D scanning of faces and other objects -- in which filaments and surfaces are caused by structures such as skin and hair -- and statistical databases in which curves and surfaces arise from existence of predictable patterns. This Focused Research Group on Multiscale Geometric Analysis is a research effort bringing together mathematicians, statisticians, image analysts and astronomers to create new tools and theories to help extract geometric structure and meaning from such databases. The participants are skilled at multiscale analysis, which will be a central organizational principle. We expect multiscale methods to have an impact here comparable to what wavelets have had over the last twenty years in many other problems in science and technology.
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1 |
2003 — 2007 |
Jones, Peter A [⬀] |
T32Activity Code Description: To enable institutions to make National Research Service Awards to individuals selected by them for predoctoral and postdoctoral research training in specified shortage areas. |
Training Program in Viral and Chemical Carcinogenesis @ University of Southern California
[unreadable] DESCRIPTION (provided by applicant): This is a renewal application for a postdoctoral training program in cancer biology based at the USC/Norris Comprehensive Cancer at the USC Keck School of Medicine. The principal goal of this program is to continue to provide Ph.D. and M.D. postdoctoral trainees with an interdisciplinary research experience of the highest quality. The program has four key elements. First and most importantly, trainees will conduct research under the supervision of a member of the Norris faculty; second, trainees will attend Grand Rounds, a seminar series that brings together clinicians and basic scientists on topics of mutual interest; third, trainees will present their work at one or more discipline-based research seminars, including eukaryotic gene regulation, signal transduction, and developmental biology; finally, trainees will have the option of attending didactic courses in a variety of subjects related to cancer biology, including courses in molecular genetics, human genetics, developmental biology, pathology, cancer biology, and computational biology. These intensive graduate-level courses, which serve as a foundation of the highly successful, multi-departmental Program in Biological and Biomedical Sciences (PIBBS), will enable trainees to enhance their background in areas of cancer biology and medicine. The proposed program, in short, will provide trainees with both the practical experience and the scientific knowledge necessary for them to function as members of an interdisciplinary team whose goal is to translate basic discoveries into clinically useful approaches. [unreadable] [unreadable]
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0.928 |
2004 — 2009 |
Smith, Alice [⬀] Jones, Peter Evans, John Weatherby, Dennis (co-PI) [⬀] |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Next Generation of Manufacturing Engineers For the Automotive Sector
The Next Generation of Manufacturing Engineers Program for the Automotive Sector Program awards scholarships to mechanical and industrial engineering students at the bachelors, masters and doctoral levels who desire to prepare for careers in automotive manufacturing. A total of 29 scholarships per year for four years are awarded. Scholarships are awarded based on financial need and academic promise with attention to developing a diverse workforce of automotive engineers.
The intellectual merit focuses on an interdisciplinary and vertically integrated approach, that is, approaching manufacturing from a total supply chain perspective that considers the vehicle from design through assembly to customer delivery.
Broader impacts includes outreach to K-12 students and teachers, and energetic recruitment and retention of women and African-American undergraduate and graduate students. Student recruitment, support and mentoring are be provided by the BellSouth Minority Engineering Program and the local student chapters of the Society of Women Engineers, the National Society of Black Engineers, the Institute of Industrial Engineers, the American Society of Mechanical Engineers and the Society of Automotive Engineers. This project ensures a supply of well-trained engineers in the growing automotive industry in the Southeast and helps encourage further development of this industry within the region, which has historically been economically and educationally disadvantaged.
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0.97 |
2005 — 2012 |
Jones, Peter Coifman, Ronald (co-PI) [⬀] |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Geometric Harmonic Analysis
Diffusion (or inference) geometries , provide tools for organization of massive digital data sets. Like differential calculus, they are used to build global inference relations between objects by combining ``infinitesimal" (linear) models. Harmonic analysis on such structures leads to multiscale folder building paradigms leading to powerful tools for functional regression and analysis of massive complex data. These geometric methods provide new insights in classical differential geometry enabling explicit embedding theorems and coordinate systems for Riemannian manifolds.
The multiscale analytic methods lead to a systematic analysis tool for seemingly unstructured data bases, and enables the automatic generation of Ontologies and data induced languages. These methods are broadly applicable in medical diagnostics, in the organization and analysis of psychological questionnaires, as well as in all aspects of machine learning from data mining to machine vision.
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1 |
2006 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Program Planning and Evaluation @ University of Southern California |
0.928 |
2006 — 2010 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Protocol Specific Research @ University of Southern California
To provide dedicated protocol specific research support for the conduct of Phase I, pilot or feasibility clinical research trials approved by the Clinical Investigations Committee (CIC) and monitored by the Quality Assurance Committee (QAC).
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0.928 |
2006 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Program Leaders of Research Programs @ University of Southern California |
0.928 |
2006 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Senior Leadership @ University of Southern California |
0.928 |
2006 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Developmental Funds @ University of Southern California
The USC Norris Comprehensive Cancer Center (NCCC) is requesting $450,000 per year in Developmental Funds to be used for new investigator support to recruit faculty level scientists in areas of strategic need and to fund innovative pilot projects. These funds will be crucial to our success as we continue to expand the number of investigators supported by the NCCC to fill the newly constructed research space in the Harlyne Norris Research Tower (HNRT). The HNRT is approximately 70% occupied and still has space for an additional 15 investigators. All recruitments are strategic to the NCCC's goals and have been discussed at our leadership retreats, approved by our Cancer Center Leadership Council and Cancer Center Executive Committee and evaluated by our External Advisory Committee. We will allocate our new investigator support annually at the recommendation of the Cancer Center Director and with the advice of our External Advisory Committee and the approval of the Executive Committee. Pilot project funds will be allocated after the review and recommendation for funding by the Leadership Council through the Whittier Initiative. Developmental Funds awarded during the past five years were allocated primarily to the recruitment of new investigators. The remaining portion was used to fund innovative pilot projects and to establish new shared resources.
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0.928 |
2006 — 2010 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Cancer Center Administration @ University of Southern California
DESCRIPTION (provided by applicant): The USC/Norris Comprehensive Cancer Center at the University of Southern California was founded in 1971 and has been continuously supported by a Cancer Center Core Support Grant since 1973. It has developed into a major regional and national resource for cancer research, treatment, prevention and education. Center facilities have been built and developed with support from the NCI, the University and the Kenneth Morris Jr. Cancer Hospital, which is an integral part of the Cancer Center. The Center underwent a major expansion with the addition of the Norman Topping Tower in 1996 and is currently building a third facility, the Harlyne Morris Translational Research Tower. The Center's 191 members are organized into five thematic and five translational research programs, including a "bridge" program in developmental therapeutics. The thematic programs are headed by senior investigators in the fields of molecular genetics, epigenetics and regulation, tumor microenvironment, cancer epidemiology and cancer control research. The translational programs, which provide focus to our multidisciplinary research efforts, are in developmental therapeutics, genitourinary cancers, gastrointestinal cancers, women's cancers and hematologic and viral-associated malignancies. This application is for: 1) partial salary support for the Center's senior leadership, who are responsible for planning and overseeing our research efforts;2) support for program planning and evaluation to keep the Center at the forefront of cancer research;3) support for the administrative infrastructure of the Center;4) partial support for the Center's 13 shared resources which facilitate the conduct of our peer-reviewed research;and 5) support for developmental funds to help us recruit new investigators in areas we have targeted for expansion. Continued funding from the Cancer Center Core Support Grant will allow us to build on our strengths in basic and population-based research and to translate our underlying expertise into strong, peer-reviewed funded research programs focused on cancers of major consequence in this country. Cancer Center members currently hold grants totaling $91 million in direct costs, with $40 million of that coming from NCI (all exclusive of the CCSG).
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0.928 |
2008 — 2012 |
Jones, Peter A [⬀] |
T32Activity Code Description: To enable institutions to make National Research Service Awards to individuals selected by them for predoctoral and postdoctoral research training in specified shortage areas. |
Training Grant in Viral and Chemical Carcinogenesis @ University of Southern California
[unreadable] DESCRIPTION (provided by applicant): This is a renewal application for a postdoctoral training program in cancer biology based at the USC/Norris Comprehensive Cancer Center at the USC Keck School of Medicine. The principal goal of this program is to continue to provide Ph.D. and M.D. postdoctoral trainees with an interdisciplinary research experience of the highest quality. The program has four key elements. First and most importantly, trainees will conduct research under the supervision of a member of the Morris faculty; second, trainees will attend Grand Rounds, a seminar series that brings together clinicians and basic scientists on topics of mutual interest; third, trainees will present their work at one or more discipline-based research seminars, including eukaryotic gene regulation, epigenetics, and developmental biology; finally, trainees will have the option of attending didactic courses in a variety of subjects related to cancer biology, including courses in molecular genetics, human genetics, developmental biology, pathology, cancer biology, responsible conduct in research (required), and computational biology. These intensive graduate-level courses, which serve as a foundation of the highly successful, multi-departmental Program in Biological and Biomedical Sciences (PIBBS), will enable trainees to enhance their background in areas of cancer biology and medicine. The proposed program, in short, will provide trainees with both the practical experience and the scientific knowledge necessary for them to function as members of an interdisciplinary team whose goal is to translate basic discoveries into clinically useful approaches. [unreadable] [unreadable] [unreadable]
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0.928 |
2010 — 2014 |
Sabin, Jenny Yang, Shu [⬀] Van Der Spiegel, Jan (co-PI) [⬀] Engheta, Nader (co-PI) [⬀] Jones, Peter Ihida-Stansbury, Kaori |
N/AActivity Code Description: No activity code was retrieved: click on the grant title for more information |
Efri-Seed: Energy Minimization Via Multi-Scaler Architectures From Cell Contractility to Sensing Materials to Adaptive Building Skins @ University of Pennsylvania
The objective of this EFRI-SEED project is to explore materiality from nano- to macroscales based upon understanding of nonlinear, dynamic human cell behaviors on geometrically-defined substrates. The insights as to how cells can modify their immediate extracellular matrix (ECM) microenvironment with minimal energy and maximal effect will lead to the biomimetic design and engineering of highly aesthetic, passive materials, and sensors and imagers that will be integrated into responsive building skins at the architectural scale. The PIs will (1) use architectural and computational algorithms to guide the design and fabrication of soft substrates with generic 1-D to 3-D geometrical patterns; (2) quantitatively measure and visualize in real-time how human pulmonary artery vascular smooth muscle cells, that interact to contract or relax these substrates to modify substrate geometry; (3) redeploy architectural and algorithmic tools, and model and simulate pattern and material manipulation resulting from nonlinear cellular behaviors so as to transfer this fine-scale design ecology to the macro-scale design of adaptive building skins; (4) apply the understanding to optimal design of materials and geometries that are responsive to environmental factors (e.g. heat, humidity and light); (5) design biomimetic sensors and control systems using CMOS and nanotechnology, and (6) transform the concept from modeling, materials manipulation, and device integration at the nano- and microscales to the design of responsive, yet passive building skins at the architectural and human scale. This project represents a unique avant garde model for sustainable design via the fusion of the architectural design studio with laboratory-based scientific research. In turn, this will benefit a diverse range of science and technologies, including the construction of energy efficient and aesthetic building skins and materials.
The project will create a significant opportunity to excite the general public, thereby provoking and engaging their interest in Science, Technology, Engineering, and Mathematics (STEM). This work will offer an effective tool to recruit and train students at all levels in a highly-integrated research and educational environment. The research results will be disseminated through: (1) (bi)weekly chalk talks and faculty retreats at Penn, annual workshops at the Mid-Atlantic region, and national conferences and workshops; (2) The website of LabStudio for new discoveries in cell science, visualization techniques, materials, fabrication, and computational modeling frameworks developed from this project; (3) Advertising the technology through the Lab-to-Market Forum and LabStudio to attract industrial interest, and (4) Installation of architectural models resulted from the research at international exhibitions. The research contains novel and synergistic activities, including: (1) the study of cellular nano- and micro-mechanics in Pathology & Laboratory Medicine (School of Medicine, SOM); (2) materials fabrication and characterization in Materials Science and Engineering (MSE; School of Applied Science & Engineering, SEAS); (3) architectural design, computational modeling, simulation and digital fabrication in design and research labs in Architecture (School of Design, SOD) and Electrical & Systems Engineering (ESE; SEAS) respectively, and (4) device fabrication and integration in labs in ESE.
The FY 2010 EFRI-SEED Topic that supports this project was sponsored by the US National Science Foundation (NSF) Directorates for Engineering (ENG), Mathematical and Physical Sciences (MPS) and Social, Behavioral and Economic Sciences (SBE), and Computer & Information Science and Engineering in collaboration with the US Department of Energy (DOE) and the US Environmental Protection Agency (EPA).
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0.97 |
2011 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Usc Norris Comprehensive Cancer Center (Core) Support @ University of Southern California
DESCRIPTION (provided by applicant): The USC Norris Comprehensive Cancer Center at the University of Southern California was founded in 1971 and has been continuously supported by a Cancer Center Support Grant since 1973. It has developed into a major regional and national resource for cancer research, treatment, prevention and education. Center facilities have been built and developed with support from the NCI, the University and the Kenneth Norris Jr. Cancer Hospital, which is an integral part of the Cancer Center. The Center underwent a major expansion with the addition of the Norman Topping Tower in 1996 and the Harlyne Norris Research Tower in 2007. The Center's 198 members are organized into five thematic and five translational research programs, including a "bridge" program in developmental therapeutics. The thematic programs are headed by senior investigators in the fields of molecular genetics, epigenetics and regulation, tumor microenvironment, cancer epidemiology and cancer control research. The translational programs, which provide focus to our transdisciplinary research efforts, are in developmental therapeutics, genitourinary cancers, gastrointestinal cancers, women's cancers and leukemia and lymphoma. This application is for: 1) partial salary support for the Center's senior leadership, who are responsible for planning and overseeing our research efforts;2) support for program planning and evaluation to keep the Center at the forefront of cancer research;3) support for the administrative infrastructure of the Center;4) partial support for the Center's 11 shared resources which facilitate the conduct of our peer-reviewed research;and 5) support for developmental funds to help us recruit new investigators in areas we have targeted for expansion and to fund innovative pilot projects. Continued funding from the Cancer Center Core Support Grant will allow us to build on our strengths in basic and population-based research and to translate our underlying expertise into strong, peer-reviewed funded research programs focused on cancers of major consequence in this country. Cancer Center members currently hold grants totaling $125 million in direct costs, with $43 million of that coming from NCI (all exclusive of the CCSG).
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Transgenic and Knockout Mouse Core @ University of Southern California
The USC Norris Comprehensive Cancer Center (NCCC) will continue in its mission to support basic research on the molecular genetic mechanisms underlying cancer, and translational research aimed at using discoveries in basic science to improve patient care. Now in its 17'*^ year of operation, the Transgenic/Knockout Mouse Core continues to offer a full range of mouse genome manipulation services. These include the targeting of genes in ES cells, the generation of gene targeted mice by injection of ES cells into blastocysts, and the production of transgenic mice by pronuclear injection. In addition to these major services, the Core also offers a number of ancillary services, including cryopreservation of embryos, rederivation of mouse strains, and in vitro fertilization. Finally, in the long term, the Core proposes to offer ES cell injection and gene knockouts in rats. In addition to funding from the Cancer Center Core Grant, the Core receives a large contribution from the Keck School of Medicine ($150,000 in FY 09-10, projected to continue yearly).The Core also receives support from the Zilkha Neurogenetics Institute ($25,000) and the College of Letters, Arts, and Sciences ($10,000). This broad support from several USC schools/Institutes leverages the CCCG contribution, enabling the Core to continue to offer a wide range of services at competitive prices to Cancer Center investigators. The Core is directed by Robert Maxson, Ph.D. Professor of Biochemistry and Molecular Biology (5% FTE). It is staffed by Dr. Nancy Wu (90%), an expert mouse embryologist and injectionist with 17 years of experience; and Dr. Mandy Ting (50%), an expert in ES cell manipulation. Assisting with all of Core functions is Youzhen Yan (90%) and John Johnson (10%). Core policies, including pricing, are set by a User's Committee in consultation with the Cancer Center Executive Committee. Outside prices are established by cost analysis. Prices for in house investigators are reduced from outside prices, depending on the academic affiliation of the investigator and the extent to which the investigator's unit supports the Core. Prices are listed online on the Core's website in the NCCC Shared Resources webpage. Also on the Core's website are order forms for Core services. The Core at its current staffing of 2.45 FTEs has the ability to perform four to six pronuclear injections, one gene targeting in ES cells, and one to two injections of ES cells into blastocysts to produce targeted mice.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Translational Pathology Core @ University of Southern California
The Translational Pathology Core provides normal and tumor tissue specimens necessary for the laboratory-based, epidemiologic and clinical studies being conducted by USC Norris Comprehensive Cancer Center (KiCCC) investigators. As cancer research increasingly utilizes human tumor specimens, the need for this service has escalated accordingly. It is now indispensable for many NCCC investigators. Tissue specimens have been provided to 24 NCCC members with active peer-reviewed research funding. The Core is organized into three arms, each with distinct but related functions; the Adult Tissue Arm (ATA) supervised by Dr. Andrew Sherrod, supplies fresh/frozen adult normal and tumor tissue and fluid specimens, the Pediatric Tissue Arm (PTA) supervised by Dr. Hiroyuki Shimada, provides pediatric tumor tissue specimens, and the Population Based Tissue Arm (PBTA) supervised by Dr. Wendy Cozen, provides population-based, fixed tissue specimens primarily for epidemiologic studies. Overall, coordination of these services is under the direction of Dr. Sue Ellen Martin. By using this Core, NCCC investigators were able to identify molecular markers associated with tumor recurrence in 250 patients with stage 2 and 3 colon cancer and 103 stage 1-3 gastric cancer, collaborated with the Broad Institute on the 8q24 pathway getting 100 fresh tissues leading to a Nature Genetics publication, publish a molecular classification of childhood rhabdomyosarcoma based on genotypic and phenotypic determinants that distinguish two major prognostic groups, and identified an ethnic-specific tumor marker for prostate cancer.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Small Animal Imaging Core @ University of Southern California
This is a new shared resource established by the use of CCSG developmental funds following the recommendation of the Cancer Center Leadership Council and the endorsement of the External Advisory Committee. The Small Animal Imaging Core serves to provide imaging modalities such as computed tomography (CT), in vivo optical (bioluminescence and fluorescence), positron emission tomography (PET), quantitative autoradiography, and ultrasound imaging to USC Norris Comprehensive Cancer Center (NCCC) investigators. By providing investigators with over $1.6 million dollars of equipment, expertise in each modality through technicians with over 10 years of small animal imaging experience, and a newly renovated facility with active waste anesthetic gas delivery and stereotactic delivery of cells, investigators have access to equipment without concern for the operational costs involved (staff, service contracts, maintenance, repair, upgrade), which are usually prohibitive for any single investigator to bear. Primary users of this shared resource are NCCC investigators with peer-reviewed, funded projects. The users share in the mission to promote novel, translatable advances in cancer research through In vivo imaging of disease processes and development of new molecular therapeutics and biomarkers for diagnostics. In order to continue this course, the Core remains committed to provide state-of-the-art technologies to support researchers' needs. Currently, the Core supports a dynamic and diverse range of research and provides flexibility in development. The varied modalities in the Small Animal Imaging Core provide a means to monitor each investigators' disease of interest through novel techniques such as gene reporter systems, cell proliferation, angiogenesis, gene therapy, cell-cell interaction in roles of proliferation and metastatic development, androgen involvement in metastatic potential, gene expression in metastatic development, and diet restriction's involvement in increasing the therapeutic effect of chemotherapies. In addition, the modalities also provide a means to produce advanced experimental methods such as orthotopic models of cancer without surgery, monitoring of response through non-invasive means, and metrics using regions of interest (ROI) to monitor disease progression and therapeutic efficacy. As a result, the funding will allow for: 1) novel and current methods to be utilized by NCCC investigators; 2) provide further opportunities to decrease costs; and 3) progress to a translational research setting with clinically relevant applications.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Biostatistics Core @ University of Southern California
The Biostatistics Core is a shared resource organized to meet the statistical needs of the USC Norris Comprehensive Cancer Center (NCCC) investigators. Statisticians in the Biostatistics Core collaborate with investigators in all the Cancer Center Programs - Molecular Genetics, Tumor Microenvironment, Epigenetics and Regulation, Developmental Therapeutics, Cancer Epidemiology, Cancer Control Research, Genitourinary Cancers, Gastrointestinal Cancers, Women's Cancers, and Leukemia and Lymphoma. The specific objectives of the Biostatistics Core are: 1. To provide statistical support to investigators at the NCCC in the design, planning, conduct, analysis, and reporting of cancer research studies 2. To ensure, in collaboration with members of the Clinical Investigations Support Office (CISO), the Cancer Research Informatics Core (CRIC), that clinical trials approved by the Clinical Investigations Committee (CIC) are properly executed and provide high quality clinical and translational research data, and ensure, in collaboration with the Translational Pathology Core (TPC) and CRIC, that disease-based databases and associated tumor banks, which serve as a resource for translational research, are supported and maintained 3. To adapt and incorporate, or develop, innovations in statistical and clinical trials methodology into the design and analysis of cancer research studies. Members of the Biostatistics Core participate in conceptualization and development of Cancer Center research projects, analyses for publications, development or adaptation of statistical methods, oversight and coordination activities, and other unanticipated needs of the Cancer Center. Core competencies include experimental design and data analysis, database design and utilization, and bioinformatics and analysis of high-throughput data. In the current application we are requesting partial funding for five Ph.D. statisticians, three M.S. level statisticians, and one Ph.D. level bioinformatician.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Cancer Research Informatics Core @ University of Southern California
The objectives of the Cancer Research Informatics Core (CRIC) are: 1. To maintain a cost-effective, user friendly and efficient cancer research informatics core resource for Cancer Center investigators. This Core oversees the computing resources needed for handling on-line clinical data entry, data from basic science experiments, and the data management and analytical requirements for epidemiological investigations, population survey and translational research. The main focus is on the design and development of research databases for the data acquisition needed for these research investigations. 2. To coordinate with the Biostatistics Core, the Translational Pathology Core, the Molecular Genomics Core and the Clinical Investigations Support Office (CISO) all research data management and applications for Cancer Center investigators. In this capacity, this Core provides user training in the use of the various databases and assists in software development, deployment, and maintenance. In addition, members of this Core consult on the acquisition of new computing equipment and software to insure its compatibility with existing hardware and software systems that are utilized for research activities. 3. To oversee and assist in the Center's caBIG deployment and adoption activities. 4. To coordinate with Cancer Center administration any data management needs for the research management and administrative needs of the Center. These activities are not funded by the CCSG.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Protocol Specific Research Support @ University of Southern California
Protocol Specific Research Support has been instrumental in allowing USC Norris Comprehensive Cancer Center (NCCC) investigators to explore Innovative concepts in the form of pilot and phase I studies. The funds provided through this mechanism are used to support clinical research staff essential for the high quality conduct of such studies, and are restricted to research nurses and data managers. The funds are dedicated to supporting studies conceived and written by NCCC investigators, and are dispensed at the recommendation of our scientific review committee (CIC), with the approval of the Cancer Center Executive Committee, and under the supervision of the Clinical Investigations Support Office (CISO). During the last grant cycle, these funds were used to support eight studies, the majority of which have been completed and resulted in larger or follow up studies with external funding. The current submission includes modified criteria for eligibility for protocol specific research support that emphasize pilot studies that are aimed to translate eariy findings from the bench to the clinic. The new criteria also stress the feasibility of completion within two years
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Bioreagent and Cell Culture Core @ University of Southern California
The Bioreagent and Cell Culture Core continues to reorganize in order to respond to priority requests from investigators and to assure stringent cost-effectiveness. As a result, the Core's budget now operates with the same budget that was approved for five years ago even though the cost of reagents increased significantly and the number of investigators served increased 50%. The following services are provided: 1) growing cells in large quantities using a BioReactor, suspension or roller bottle cultures; 2) preparing monoclonal antibodies; 3) performing tests for mycoplasma contamination using an enzyme assay from Cambrex or the PCR method and curing contaminated cell lines; 4) producing LB agar plates with antibiotics; 5) performing or assisting with CytoTox 96 cytotoxicity assays; 6) quality testing before bulk purchase of fetal bovine serum, bovine calf serum, charcoal treated or dialyzed serum and making it available at substantial savings; 7) testing and bulk purchasing the most commonly used cell culture media for distribution and preparation of only the unusual, high priced media or commercially unavailable media with substantial savings; 8) large scale preparation of cell culture additives like Trypsin-EDTA, Hepes buffer, penicillin/streptomycin; 9) centralized management and distribution of reagents for molecular biology from Sigma-Aldrich Co. and Roche Biotech Co.; and 10) performing special sterilization procedures, monitoring autoclaves, water quality, assisting in testing sterile hoods, incubator decontamination and calibration, training new technicians or graduate students in specialized techniques used in cell culture. This Core opened 35 years ago and provided uninterrupted service ever since. It is one of the longest standing and most widely used shared resources with distribution facilities in six locations: two in the Norris Headquarter Buildings (Ezralow and Hariyne Norris Research Tower) and one in each the Cancer Research Laboratories, the Hoffman Medical Research building, the Clinical Sciences Building, and the Children¿s Hospital Los Angeles. Currently 113 faculty members utilize the services annually. More than 80% of the users are Cancer Center members with peer-reviewed grant support. The services provide substantial savings of 35% to 55% for the investigators. The Core supports all cancer related research that requires in vitro experiments by preparing and distributing reagents used in cell cultures and in molecular biology, and by performing technically challenging culture procedures and quality control assays. The Core is regularly reviewed by an Oversight Committee and frequently modified to suit the investigators' needs.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Administrative Core @ University of Southern California
The Cancer Center Administration staff is responsible for the administrative, fiscal, information and facilities management that support the research efforts of the Cancer Center. Ms. Janet Villarmia is the chief administrative and financial officer of the USC Norris Comprehensive Cancer Center (NCCC). As the Associate Director for Administration and Education and a senior leader of the Cancer Center, she is a member of the Executive Committee and the Cancer Center Leadership Council. Ms. Villarmia has primary responsibility for the management of the Cancer Center Support Grant (CCSG). She supervises the work of the Cancer Center Business Office and the Cancer Center Administrative Office. Ms. Villarmia's other administrative responsibilities include oversight of the Cancer Research Informatics Core, Facilities Management, the Patient Education and Community Outreach Center, the Cancer Survivorship Advisory Council and membership on the Informatics Working Group. She oversees, in overlap with the other Associate Directors, the operations of the CCSG funded shared resources. The Cancer Center Business Office is responsible for the day-to-day accounting of all the CCSG budgets as well as the management of the research and gift accounts of many of the investigators housed in NCCC space. The office consists of a Senior Business Officer and 5.6 FTE accountants and analysts. The primary business goal of the Cancer Center Business Office is to promote a business environment that maximizes research and minimizes administrative burden for NCCC investigators and lab personnel without compromising solid stewardship of funds. The personnel in the Cancer Center Administrative Office provide administrative and secretarial support to the Cancer Center Director and the various Associate Directors, research program leaders, shared resources directors as well as to various NCCC committees and advisory groups. They organize NCCC seminars, retreats, conferences, meetings, and poster sessions. We are requesting $598,180 in the first year to support the Cancer Center Administration, which represents 20% of the annual total cost of the administration and 9% ofthe total Year 1 CCSG budget.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Clinical Investigations @ University of Southern California
The Clinical Investigations Support Office (CISO) serves as a centralized unit to oversee the clinical research infrastructure and assist investigators in their conduct of clinical trials and translational research projects. CISO has been reorganized following recommendations of an external advisory group and the Cancer Center Executive Committee and with the concurrence of the NCCC External Advisory Committee. The 58 personnel are now located in 15 of newly remodeled contiguous offices in the Ezralow Tower of the Cancer Center. CISO has three main operational units: 1) Protocol Administration; 2) Protocol Implementation; and 3) Administrative/Business Management. The Protocol Administration Unit provides the centralized consultation and regulatory services necessary for the design, initiation, and conduct of externally-funded and internally peer-reviewed and monitored clinical trials. The Protocol Implementation Unit provides the centralized function of advising and assisting investigators in patient eligibility assessments, patient recruitment, data quality control and oversight. The Administrative/Business Management Unit was recently created in 2007 in order to coordinate CISO's relationships with institutional entities outside the Cancer Center and with sponsors. It is also charged with assisting the various investigators and sections to manage their funding sources, allocate their financial and human resources in an efficient manner, and be able to make sound projections for future growth. In addition to the services provided by the three operational units, CISO plays a central coordinating role within the Cancer Center through its interactions with other cores such as the Biostatistics Core, the Cancer Research Informatics Core (CRIC), the Translational Pathology Core, and the Protocol Review and Monitoring System entities (Clinical Investigations Committee and the Quality Assurance and Monitoring Committee). The Cancer Center Executive Committee exercises full authority over CISO policies and resource allocation and reviews the CISO's performance on a biannual basis.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Program Planning @ University of Southern California
The USC Norris Comprehensive Cancer Center (NCCC) has a well-developed system for program planning and evaluation and uses several kinds of activities, supported in part by the CCSG Program Planning and Evaluation budget, to conduct its program planning and evaluation: meetings with its External Advisory Committee; offsite retreats for the overall Cancer Center Leadership plus invited guests and for specific programs; monthly meetings of the Executive Committee (the Director, Associate Directors, Medical Director of the Norris Cancer Hospital, and Chair of the Cancer Survivorship Advisory Council); and monthly meetings of the Cancer Center Leadership Council {\.he Director, Associate Directors and Program Leaders). In compliance with the CCSG Guidelines, we plan to convene a meeting of our External Advisory Committee once a year. This will enable the EAC to be closely involved in our planning and implementation processes and better able to evaluate our accomplishments. We are requesting sufficient funds to bring each of them or a substitute ad hoc reviewer to the Cancer Center annually. We will continue to support our monthly Leadership Council dinner meetings to facilitate the scientific exchanges and program planning. We also plan to continue the biennial off-site Leadership Retreat in 2010 (with the current CCSG budget), 2012 and 2014. These retreats have been extremely valuable in setting the Cancer Center's scientific agenda, building camaraderie among Center leaders and encouraging collaborative inter-programmatic research. In the intervening years, we will support retreats for programs, either individually or jointly.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Cell and Tissue Imaging Core @ University of Southern California
The Cell and Tissue Imaging Core Facility is entering its 11th year of operation. David R. Hinton M.D., Core Director, and Ernesto Barron, Core Manager, have led this shared use facility since its inception. Its mission, as developed by the Cancer Center Executive Committee, is to provide advanced cell and tissue imaging technology, services, and scientific consultation that facilitate scientific interaction and enhance scientific productivity of Cancer Center investigators. The Core was originally developed as a partnership between the USC Norris Comprehensive Cancer Center (NCCC) and the Doheny Eye Institute; in the past two years, the University of Southern California has joined the partnership by providing $1.5 million in Provost funds for purchase of new equipment. The 2,000 sq. ft. laboratory space for this Core is provided by the Doheny Eye Institute at no cost to the Cancer Center. Cancer Center users have priority access to the Core. All funds requested in this proposal are for costs directly related to the Cancer Center's use of the Core. The major users of this Core are Cancer Center investigators with peer-reviewed, funded projects. The Core is also open to other Cancer Center members who need its facilities to develop pilot project data. In the past year, the Core was utilized by 36 investigators from nine Cancer Center programs. The Facility is open 24 hours/day, 7 days/week, with on-site technical support available 9 hrs/day (weekdays). In the past funding period, the Core has successfully implemented an online chargeback system for user fees. A wide range of sophisticated imaging equipment and services is available to Cancer Center members including laser scanning confocal/multiphoton microscopy, live cell spinning disk confocal imaging, transmission electron microscopy (TEM), scanning electron microscopy (SEM), digital light and fluorescence microscopy, fluorescence and bright field laser capture microdissection, thin sectioning, cryo-sectioning and embedding techniques, and computer aided graphics. In 2008, institutional funds from the USC Provost were obtained to obtain new state of the art instruments including a PerkinEImer Spinning Disk Confocal with live cell imaging capability, a JEOL 200kV JEM-2100 LaB6 TEM with cryo-EM capability, and a JEOL JSM- 6390V/LGS Variable Pressure SEM. In the past 18 months, we have provided extensive training on the new equipment including nine group training sessions and >100 individual training sessions. We are continuously upgrading our imaging software (Velocity, deconvolution) and advising and instructing Cancer Center investigators in novel methodologies and protocols to enhance their cell and tissue imaging capability.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Flow Cytometry Core @ University of Southern California
The Flow Cytometry Core provides USC Norris Comprehensive Cancer Center (NCCC) members access to advanced multi-parameter cellular analytic and cell sorting capabilities which includes the fluorescence activated cell sorting (FACS) of various populations of cells to provide a purified cell population for the researchers. The Core also provides expertise to allow investigators to analyze the expression of various markers on a cell population using fluorescence-based methods. This Core is administratively managed by the Broad Center for Regenerative Medicine and Stem Cell Research (CSCRM). Previously, flow cytometry services were provided by a core facility managed by NCCC with partial support from the CCSG. On December 1, 2009, NCCC partnered with CSCRM, who took over the provision of flow cytometry services to NCCC investigators under the directorship of Dr. Gregor Adams. This transition was a direct result of the NCCC Executive Committee's review of its shared resources in mid-2008, and endorsed by the NCCC's External Advisory Committee in April 2009. The partnership benefits NCCC investigators by giving them access to newer cytometers with more capabilities, as well as a reduction in the cost of flow cytometry services. The Core currently maintains four cytometers, a Beekman Coulter CyAn analyzer, a BD LSR II analyzer, BD FACSAria and BD FACSAria 11 cell sorters. The LSRII and the Aria are equipped with a 350 nm UV, a 488 nm argon laser, and a 635 nm red diode laser, while the Aria II is equipped with a 405 nm violet laser, a 488 nm argon laser and a 633 nm red diode laser, which will ultimately permit up to 15 parameter analysis.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Leaders of Scientific Research Programs @ University of Southern California
The Program Leaders of the USC Norris Comprehensive Cancer Center (NCCC) promote both intra and inter-programmatic transdisciplinary research through careful selection of program members and recruitment of new faculty, coordination of interactions between program members and with other NCCC members, as well as facilitation and leadership of transdisciplinary and multi-project research applications, seminars, mini-symposia, program retreats and program meetings. This transdisciplinary emphasis is reinforced by having two leaders from different disciplines for most programs. For those programs with two leaders (Epigenetics and Regulation, Tumor Microenvironment, Cancer Epidemiology, Cancer Control Research, Developmental Therapeutics, Genitourinary Cancers, Gastrointestinal Cancers, Leukemia and Lymphoma and Women's Cancers) we are requesting support for 7.5% effort for each leader and for one program (Molecular Genetics) with one leader we are asking support for 10% effort.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Molecular Genomics Core @ University of Southern California
The Molecular Genomics Core (MGC) is a recent fusion of the DNA Core (formerly the Microchemical Core, founded in 1984), the Genomics Core founded in 1999 and the Microarray Core founded in 1998. This consolidation will ensure that there is no duplication in the acquisition of expensive state-of-the-art equipment and that the resource functions in an efficient and seamless manner to provide optimized service to investigators. The merger reduced redundancies in services, provided a single structure, which allowed the Core to adjust to changing demands over time and facilitated the interaction of shared expertise. MGC services are broadly divided into two main categories - sample preparation and analytical assays. Biospecimen processing is available for research and clinical samples from body fluids, fresh and archival tissue, and non-mammalian sources. The assays available in the MGC are based on the ability to identify and interrogate genetic (DNA profiling) and epigenetic (epigenetic profiling) variation. Additionally, expression profiling encompassing both genetic and epigenetic components is provided, including direct changes to DNA sequences effecting gene expression levels and DNA methylation and histone modifications playing roles in modified gene expression levels through changes in chromatin structure. The MGC has already provided services (annual total budget of >$6.5 million) to more than 175 USC Norris Comprehensive Cancer Center (NCCC) members at different levels of throughput, including single base resolution of the entire genome and single base interrogation of DNA, RNA and chromatin.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Data and Safety Monitoring @ University of Southern California
The NCI mandates that all CCSG-funded cancer centers establish a protocol review and monitoring system for oversight of the clinical research conducted at that institution. At the USC Norris Comprehensive Cancer Center (NCCC), the Clinical Investigations Committee (CIC) has this responsibility. The CIC performs the scientific review of proposed cancer clinical studies, and oversees the conduct of all clinical studies open at the NCCC. To facilitate the oversight of open trials, the internal Data and Safety Monitoring Committee (DSMC) will meet monthly to review institutional clinical trials (i.e., cancer-related trials involving an intervention where a USC investigator is the overall principal investigator (PI) of the study and the trial is not part of a national cooperative group or the CCCP Consortium, or sponsored by a pharmaceutical company that will responsible for the data management and oversight of the progress of the trial). Each in-house trial will be reviewed annually or more frequently as determined by the CIC. The reviews of the DSMC complement those of the QAMC, a long-standing Cancer Center committee which is charged with (among other responsibilities) reviewing accrual, and SAE's and major violations in a timely fashion, for all open protocols, as well as conducting audits of patients on institutional clinical trials. The DSMC will work in close coordination with the Clinical Investigations Support Office, which plays the main role of coordinating the conduct of clinical research in the NCCC. The objectives of the DSMC are to: 1. review, at established intervals, safety and aspects of the protocol and study conduct that may impact on safety of current or future patients 2. review all serious adverse events or other adverse events that require expedited reporting 3. review the progress of the study 4. review efficacy results that trigger early closure or early reporting 5. review the completeness and the quality of the research data 6. report the results of each review and make recommendations to the study PI, IRB, the Quality Assurance Monitoring Committee and the CIC.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Molecular and Cell Biology Core @ University of Southern California
The Molecular and Cell Biology (MCB) Support Core provides USC Norris Comprehensive Cancer Center (NCCC) researchers with glassware washing/sterilizing services and shared equipment purchase/ maintenance to support their molecular biology, cell biology, biochemistry, and molecular epidemiology experiments. The MCB Support Core: 1) washes/sterilizes glassware for individual research laboratories, and for the Bioreagent and Cell Culture Core for preparation of sterile culture media; 2) prepares doubly deionized, distilled water in large quantities for preparation of reagents for molecular biology, cell biology, and biochemistry experiments; 3) supervises/maintains all shared equipment; 4) negotiates/administers service contracts for shared, heavily-used, expensive pieces of lab equipment (ECL-Western apparatus, phosphorimagers, ultracentrifuges. X-ray film developers, liquid scintillation counters, shared fluorescent microscopes); 5) provides centralized supplies, including carbon dioxide, liquid nitrogen, and dry ice to NCCC labs; 6) maintains freezers, refrigerators, balances, and spectrophotometers in individual labs; and 7) assesses needs/makes recommendations for purchase of new shared equipment/services. The Glassware Facilities of the MCB Support Core are located at four sites: Ezralow Tower (1,300 sq. ft., three autoclaves, three glassware washers), Harlyne Norris Research Tower (HNRT, 1,600 sq. ft., four glassware washers, three autoclaves). Cancer Research Laboratories (CRL, 1,000 sq. ft., one autoclave, one glassware washer), and Smith Research Tower at Childrens Hospital Los Angeles (CHLA, 1,000 sq. ft., one autoclave, one glassware washer). This shared resource serves 94 Cancer Center members and their research groups, 500 scientists/technical staff total. The CCSG supports 4.0 FTE glassware technicians; another two are supported by chargebacks. The MCB Support Core is supervised by Dr. Joseph R. Landolph, Associate Professor of Molecular Microbiology and Immunology. Mr. Kent Chang manages the Norris Headquarters and CRL. Dr. Ambrose Jong supervises CHLA facilities. In 2008, we occupied the 103,000 n.s.f. HNRT, housing 40 new Pis. As a result, this MCB Support Core has experienced a very large increased demand for services beginning in 2008 and continuing to the present time.
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0.928 |
2012 — 2015 |
Jones, Peter A [⬀] |
P30Activity Code Description: To support shared resources and facilities for categorical research by a number of investigators from different disciplines who provide a multidisciplinary approach to a joint research effort or from the same discipline who focus on a common research problem. The core grant is integrated with the center's component projects or program projects, though funded independently from them. This support, by providing more accessible resources, is expected to assure a greater productivity than from the separate projects and program projects. |
Protocol Review and Monitoring System @ University of Southern California
The objectives of the Protocol Review and Monitoring System (PRMS) are to implement a transdisciplinary scientific peer-review system that ensures internal oversight of the scientific quality and progress of clinical trials and optimally engages the institution's resources. The system ensures that clinical research trials at the USC Norris Comprehensive Cancer Center (NCCC) are of the highest scientific quality and integrity by review of the scientific merit, priorities and progress. The specific aims of the PRMS are to: 1) maintain a Clinical Investigation Committee (CIC) with sufficient breadth of expertise to conduct an objective scientific and operational review of all clinical cancer research protocols; 2) facilitate prompt initiation of approved protocols by interfacing with the IRB to ensure compliance with local and federal regulations; 3) ensure and oversee a system of prioritization of clinical research protocols; 4) monitor the scientific progress of clinical research protocols and ensure closure as required by interim analysis and stopping rules; 5) terminate clinical research protocols that fail to meet expectations for scientific progress; 6) review amendments for ongoing clinical research protocols; and 7) monitor compliance as well as gender and minority accrual in the conduct of clinical research protocols. The CIC is assisted by the Quality Assurance and Monitoring Committee (QAMC) in the monitoring of the progress of the studies. The QAMC fulfills multiple functions as detailed in the CISO section of the grant. In regards to PRMS, it provides the essential data regarding accrual and study conduct to the CIC, which allows the latter to perform its progress monitoring functions optimally.
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0.928 |