| Year |
Citation |
Score |
| 2020 |
Marangoni JM, Wu SC, Fogen D, Wong SL, Ng KKS. Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity. Scientific Reports. 10: 12483. PMID 32719366 DOI: 10.1038/S41598-020-69357-5 |
0.391 |
|
| 2019 |
Wu SC, Wang C, Chin J, Wong SL. A bio-coupling approach using a dextran-binding domain to immobilize an engineered streptavidin to Sephadex for easy preparation of affinity matrix. Scientific Reports. 9: 3359. PMID 30833609 DOI: 10.1038/S41598-019-40044-4 |
0.421 |
|
| 2017 |
Wu SC, Wang C, Hansen D, Wong SL. A simple approach for preparation of affinity matrices: Simultaneous purification and reversible immobilization of a streptavidin mutein to agarose matrix. Scientific Reports. 7: 42849. PMID 28220817 DOI: 10.1038/Srep42849 |
0.447 |
|
| 2015 |
Fogen D, Wu SC, Ng KK, Wong SL. Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling. Plos One. 10: e0139137. PMID 26406477 DOI: 10.1371/Journal.Pone.0139137 |
0.434 |
|
| 2013 |
Wu SC, Wong SL. Structure-guided design of an engineered streptavidin with reusability to purify streptavidin-binding peptide tagged proteins or biotinylated proteins. Plos One. 8: e69530. PMID 23874971 DOI: 10.1371/Journal.Pone.0069530 |
0.449 |
|
| 2013 |
Barrette-Ng IH, Wu SC, Tjia WM, Wong SL, Ng KK. The structure of the SBP-Tag-streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits. Acta Crystallographica. Section D, Biological Crystallography. 69: 879-87. PMID 23633599 DOI: 10.1107/S0907444913002576 |
0.306 |
|
| 2012 |
Phuong ND, Jeong YS, Selvaraj T, Kim SK, Kim YH, Jung KH, Kim J, Yun HD, Wong SL, Lee JK, Kim H. Production of XynX, a large multimodular protein of thermoanaerobacterium sp., by protease-deficient Bacillus subtilis strains [corrected]. Applied Biochemistry and Biotechnology. 168: 375-82. PMID 22729758 DOI: 10.1007/S12010-012-9781-X |
0.353 |
|
| 2012 |
O'Sullivan VJ, Barrette-Ng I, Hommema E, Hermanson GT, Schofield M, Wu SC, Honetschlaeger C, Ng KK, Wong SL. Development of a tetrameric streptavidin mutein with reversible biotin binding capability: engineering a mobile loop as an exit door for biotin. Plos One. 7: e35203. PMID 22536357 DOI: 10.1371/Journal.Pone.0035203 |
0.399 |
|
| 2012 |
Liew PX, Wang CL, Wong SL. Functional characterization and localization of a Bacillus subtilis sortase and its substrate and use of this sortase system to covalently anchor a heterologous protein to the B. subtilis cell wall for surface display. Journal of Bacteriology. 194: 161-75. PMID 22020651 DOI: 10.1128/Jb.05711-11 |
0.339 |
|
| 2012 |
Phuong ND, Jeong YS, Selvaraj T, Kim SK, Kim YH, Jung KH, Kim J, Yun HD, Wong S, Lee J, Kim H. Production of XynX, a Large Multimodular Protein of Clostridium thermocellum, by Protease-Deficient Bacillus subtilis Strains Applied Biochemistry and Biotechnology. 168: 375-382. DOI: 10.1007/S12010-012-9900-8 |
0.356 |
|
| 2009 |
Wu SC, Ng KK, Wong SL. Engineering monomeric streptavidin and its ligands with infinite affinity in binding but reversibility in interaction. Proteins. 77: 404-12. PMID 19425108 DOI: 10.1002/Prot.22446 |
0.375 |
|
| 2008 |
Chen CL, Wu SC, Tjia WM, Wang CL, Lohka MJ, Wong SL. Development of a LytE-based high-density surface display system in Bacillus subtilis. Microbial Biotechnology. 1: 177-90. PMID 21261835 DOI: 10.1111/J.1751-7915.2007.00017.X |
0.363 |
|
| 2007 |
Arai T, Matsuoka S, Cho HY, Yukawa H, Inui M, Wong SL, Doi RH. Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation. Proceedings of the National Academy of Sciences of the United States of America. 104: 1456-60. PMID 17244702 DOI: 10.1073/Pnas.0610740104 |
0.335 |
|
| 2006 |
Wu SC, Wong SL. Intracellular production of a soluble and functional monomeric streptavidin in Escherichia coli and its application for affinity purification of biotinylated proteins. Protein Expression and Purification. 46: 268-73. PMID 16289701 DOI: 10.1016/J.Pep.2005.10.006 |
0.39 |
|
| 2004 |
Cho HY, Yukawa H, Inui M, Doi RH, Wong SL. Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800. Applied and Environmental Microbiology. 70: 5704-7. PMID 15345466 DOI: 10.1128/Aem.70.9.5704-5707.2004 |
0.356 |
|
| 2004 |
Wu SC, Wong SL. Development of an enzymatic method for site-specific incorporation of desthiobiotin to recombinant proteins in vitro. Analytical Biochemistry. 331: 340-8. PMID 15265740 DOI: 10.1016/J.Ab.2004.03.056 |
0.396 |
|
| 2003 |
Watanabe S, Hamano M, Kakeshita H, Bunai K, Tojo S, Yamaguchi H, Fujita Y, Wong SL, Yamane K. Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons. Journal of Bacteriology. 185: 4816-24. PMID 12897001 DOI: 10.1128/Jb.185.16.4816-4824.2003 |
0.371 |
|
| 2002 |
Qureshi MH, Wong SL. Design, production, and characterization of a monomeric streptavidin and its application for affinity purification of biotinylated proteins. Protein Expression and Purification. 25: 409-15. PMID 12182820 DOI: 10.1016/S1046-5928(02)00021-9 |
0.415 |
|
| 2002 |
Wu SC, Yeung JC, Duan Y, Ye R, Szarka SJ, Habibi HR, Wong SL. Functional production and characterization of a fibrin-specific single-chain antibody fragment from Bacillus subtilis: effects of molecular chaperones and a wall-bound protease on antibody fragment production. Applied and Environmental Microbiology. 68: 3261-9. PMID 12089002 DOI: 10.1128/Aem.68.7.3261-3269.2002 |
0.36 |
|
| 2002 |
Braaz R, Wong SL, Jendrossek D. Production of PHA depolymerase A (PhaZ5) from Paucimonas lemoignei in Bacillus subtilis. Fems Microbiology Letters. 209: 237-41. PMID 12007811 DOI: 10.1111/J.1574-6968.2002.Tb11137.X |
0.379 |
|
| 2002 |
Wu SC, Yeung JC, Hwang PM, Wong SL. Design, production, and characterization of an engineered biotin ligase (BirA) and its application for affinity purification of staphylokinase produced from Bacillus subtilis via secretion. Protein Expression and Purification. 24: 357-65. PMID 11922751 DOI: 10.1006/Prep.2001.1583 |
0.412 |
|
| 2002 |
Wu SC, Hassan Qureshi M, Wong SL. Secretory production and purification of functional full-length streptavidin from Bacillus subtilis. Protein Expression and Purification. 24: 348-56. PMID 11922750 DOI: 10.1006/Prep.2001.1582 |
0.429 |
|
| 2002 |
Wu SC, Wong SL. Engineering of a Bacillus subtilis strain with adjustable levels of intracellular biotin for secretory production of functional streptavidin. Applied and Environmental Microbiology. 68: 1102-8. PMID 11872456 DOI: 10.1128/Aem.68.3.1102-1108.2002 |
0.44 |
|
| 2002 |
Murashima K, Chen CL, Kosugi A, Tamaru Y, Doi RH, Wong SL. Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes. Journal of Bacteriology. 184: 76-81. PMID 11741846 DOI: 10.1128/Jb.184.1.76-81.2002 |
0.378 |
|
| 2001 |
Qureshi MH, Yeung JC, Wu S, Wong S. Development and Characterization of a Series of Soluble Tetrameric and Monomeric Streptavidin Muteins with Differential Biotin Binding Affinities Journal of Biological Chemistry. 276: 46422-46428. PMID 11584006 DOI: 10.1074/Jbc.M107398200 |
0.414 |
|
| 2001 |
Poon KK, Chu JC, Wong SL. Roles of glucitol in the GutR-mediated transcription activation process in Bacillus subtilis: glucitol induces GutR to change its conformation and to bind ATP. The Journal of Biological Chemistry. 276: 29819-25. PMID 11390381 DOI: 10.1074/Jbc.M100905200 |
0.622 |
|
| 2001 |
Poon KK, Chen CL, Wong SL. Roles of glucitol in the GutR-mediated transcription activation process in Bacillus subtilis: tight binding of GutR to tis binding site. The Journal of Biological Chemistry. 276: 9620-5. PMID 11118449 DOI: 10.1074/Jbc.M009864200 |
0.623 |
|
| 2001 |
Kim J, Wong S, Kim B. Optimization of staphylokinase production inBacillus subtilis using inducible and constitutive promoters Biotechnology and Bioprocess Engineering. 6: 167-172. DOI: 10.1007/Bf02932545 |
0.314 |
|
| 1999 |
Wu SC, Wong SL. Development of improved pUB110-based vectors for expression and secretion studies in Bacillus subtilis Journal of Biotechnology. 72: 185-195. PMID 12680365 DOI: 10.1016/S0168-1656(99)00101-7 |
0.333 |
|
| 1999 |
Ye R, Kim J, Kim B, Szarka S, Sihota E, Wong S. High-level secretory production of intact, biologically active staphylokinase from Bacillus subtilis Biotechnology and Bioengineering. 62: 87-96. PMID 10099516 DOI: 10.1002/(Sici)1097-0290(19990105)62:1<87::Aid-Bit10>3.0.Co;2-I |
0.41 |
|
| 1999 |
David PS, Morrison MR, Wong SL, Hill BC. Expression, purification, and characterization of recombinant forms of membrane-bound cytochrome c-550nm from Bacillus subtilis. Protein Expression and Purification. 15: 69-76. PMID 10024472 DOI: 10.1006/Prep.1998.1001 |
0.315 |
|
| 1998 |
Wu S, Ye R, Wu X, Ng S, Wong S. Enhanced Secretory Production of a Single-Chain Antibody Fragment from Bacillus subtilis by Coproduction of Molecular Chaperones Journal of Bacteriology. 180: 2830-2835. PMID 9603868 DOI: 10.1128/Jb.180.11.2830-2835.1998 |
0.326 |
|
| 1998 |
Wu X, Ye R, Duan Y, Wong S. Engineering of Plasmin-Resistant Forms of Streptokinase and Their Production in Bacillus subtilis: Streptokinase with Longer Functional Half-Life Applied and Environmental Microbiology. 64: 824-829. PMID 9501422 DOI: 10.1128/Aem.64.3.824-829.1998 |
0.364 |
|
| 1995 |
Wong SL. Advances in the use of Bacillus subtilis for the expression and secretion of heterologous proteins Current Opinion in Biotechnology. 6: 517-522. PMID 7579663 DOI: 10.1016/0958-1669(95)80085-9 |
0.354 |
|
| 1995 |
Yuan G, Wong SL. Regulation of groE expression in Bacillus subtilis: The involvement of the σ(A)-like promoter and the roles of the inverted repeat sequence (CIRCE) Journal of Bacteriology. 177: 5427-5433. PMID 7559325 DOI: 10.1128/Jb.177.19.5427-5433.1995 |
0.307 |
|
| 1994 |
Ye R, Rehemtulla SN, Wong SL. Glucitol induction in Bacillus subtilis is mediated by a regulatory factor, GutR Journal of Bacteriology. 176: 3321-3327. PMID 8195087 DOI: 10.1128/Jb.176.11.3321-3327.1994 |
0.43 |
|
| 1994 |
Ye R, Wong SL. Transcriptional regulation of the Bacillus subtilis glucitol dehydrogenase gene Journal of Bacteriology. 176: 3314-3320. PMID 8195086 DOI: 10.1128/Jb.176.11.3314-3320.1994 |
0.347 |
|
| 1994 |
Wong SL, Ye R, Nathoo S. Engineering and production of streptokinase in a Bacillus subtilis expression-secretion system Applied and Environmental Microbiology. 60: 517-523. PMID 8135514 DOI: 10.1128/Aem.60.2.517-523.1994 |
0.405 |
|
| 1994 |
Wetmore DR, Wong S, Roche RS. The efficiency of processing and secretion of the thermolysin-like neutral protease from Bacillus cereus does not require the whole prosequence, but does depend on the nature of the amino acid sequence in the region of the cleavage site. Molecular Microbiology. 12: 747-759. PMID 8052127 DOI: 10.1111/J.1365-2958.1994.Tb01062.X |
0.345 |
|
| 1993 |
Wu XC, Ng SC, Near RI, Wong SL. Efficient production of a functional single-chain antidigoxin antibody via an engineered Bacillus subtilis expression-secretion system. Bio/Technology (Nature Publishing Company). 11: 71-6. PMID 7763487 DOI: 10.1038/Nbt0193-71 |
0.413 |
|
| 1992 |
Wetmore DR, Wong S, Roche RS. The role of the pro‐sequence in the processing and secretion of the thermolysin‐like neutral protease from Bacillus cereus Molecular Microbiology. 6: 1593-1604. PMID 1495388 DOI: 10.1111/J.1365-2958.1992.Tb00884.X |
0.384 |
|
| 1992 |
Li M, Wong SL. Cloning and characterization of the groESL operon from Bacillus subtilis Journal of Bacteriology. 174: 3981-3992. PMID 1350776 DOI: 10.1128/Jb.174.12.3981-3992.1992 |
0.385 |
|
| 1991 |
Tran L, Wu XC, Wong SL. Cloning and expression of a novel protease gene encoding an extracellular neutral protease from Bacillus subtilis Journal of Bacteriology. 173: 6364-6372. PMID 1917867 DOI: 10.1128/Jb.173.20.6364-6372.1991 |
0.42 |
|
| 1991 |
Wu X, Lee W, Tran L, Wong S. Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases. Journal of Bacteriology. 173: 4952-4958. PMID 1907264 DOI: 10.1128/Jb.173.16.4952-4958.1991 |
0.401 |
|
| 1991 |
He XS, Shyu YT, Nathoo S, Wong SL, Doi RH. Construction and use of a Bacillus subtilis mutant deficient in multiple protease genes for the expression of eukaryotic genes. Annals of the New York Academy of Sciences. 646: 69-77. PMID 1809207 DOI: 10.1111/J.1749-6632.1991.Tb18565.X |
0.303 |
|
| 1989 |
Wong SL. Development of an inducible and enhancible expression and secretion system in Bacillus subtilis Gene. 83: 215-223. PMID 2511081 DOI: 10.1016/0378-1119(89)90107-8 |
0.374 |
|
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