Eric V. Shusta - Publications

Affiliations: 
University of Wisconsin, Madison, Madison, WI 
Area:
Chemical Engineering, Microbiology Biology

79 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2019 Canfield SG, Stebbins MJ, Faubion MG, Gastfriend BD, Palecek SP, Shusta EV. Correction to: An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons. Fluids and Barriers of the Cns. 16: 31. PMID 31506073 DOI: 10.1186/s12987-019-0151-8  0.8
2019 Canfield SG, Stebbins MJ, Faubion MG, Gastfriend BD, Palecek SP, Shusta EV. An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons. Fluids and Barriers of the Cns. 16: 25. PMID 31387594 DOI: 10.1186/s12987-019-0145-6  0.8
2019 Bradley RA, Shireman J, McFalls C, Choi J, Canfield SG, Dong Y, Liu K, Lisota B, Jones JR, Petersen A, Bhattacharyya A, Palecek SP, Shusta EV, Kendziorski C, Zhang SC. Regionally specified human pluripotent stem cell-derived astrocytes exhibit different molecular signatures and functional properties. Development (Cambridge, England). PMID 31189664 DOI: 10.1242/dev.170910  0.8
2019 Umlauf BJ, Clark PA, Lajoie JM, Georgieva JV, Bremner S, Herrin BR, Kuo JS, Shusta EV. Identification of variable lymphocyte receptors that can target therapeutics to pathologically exposed brain extracellular matrix. Science Advances. 5: eaau4245. PMID 31106264 DOI: 10.1126/sciadv.aau4245  0.52
2019 Stebbins MJ, Gastfriend BD, Canfield SG, Lee MS, Richards D, Faubion MG, Li WJ, Daneman R, Palecek SP, Shusta EV. Human pluripotent stem cell-derived brain pericyte-like cells induce blood-brain barrier properties. Science Advances. 5: eaau7375. PMID 30891496 DOI: 10.1126/sciadv.aau7375  0.8
2019 Umlauf BJ, Shusta EV. Exploiting BBB disruption for the delivery of nanocarriers to the diseased CNS. Current Opinion in Biotechnology. 60: 146-152. PMID 30849699 DOI: 10.1016/j.copbio.2019.01.013  0.36
2019 Qian T, Hernday SE, Bao X, Olson WR, Panzer SE, Shusta EV, Palecek SP. Directed Differentiation of Human Pluripotent Stem Cells to Podocytes under Defined Conditions. Scientific Reports. 9: 2765. PMID 30808965 DOI: 10.1038/s41598-019-39504-8  0.8
2018 Goulatis LI, Ramanathan R, Shusta EV. Impacts of the -1 Amino Acid on Yeast Production of Protein-Intein Fusions. Biotechnology Progress. PMID 30341810 DOI: 10.1002/btpr.2736  0.44
2018 Gastfriend BD, Palecek SP, Shusta EV. Modeling the blood-brain barrier: Beyond the endothelial cells. Current Opinion in Biomedical Engineering. 5: 6-12. PMID 29915815 DOI: 10.1016/j.cobme.2017.11.002  0.8
2018 Al-Ahmad AJ, Patel R, Palecek SP, Shusta EV. Hyaluronan impairs the barrier integrity of brain microvascular endothelial cell through a CD44-dependent pathway. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism. 271678X18767748. PMID 29589805 DOI: 10.1177/0271678X18767748  0.8
2017 Zorniak M, Clark PA, Umlauf BJ, Cho Y, Shusta EV, Kuo JS. Yeast display biopanning identifies human antibodies targeting glioblastoma stem-like cells. Scientific Reports. 7: 15840. PMID 29158489 DOI: 10.1038/s41598-017-16066-1  0.36
2017 Qian T, Maguire SE, Canfield SG, Bao X, Olson WR, Shusta EV, Palecek SP. Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells. Science Advances. 3: e1701679. PMID 29134197 DOI: 10.1126/sciadv.1701679  0.8
2017 Kim BJ, Bee OB, McDonagh MA, Stebbins MJ, Palecek SP, Doran KS, Shusta EV. Modeling Group B Streptococcus and Blood-Brain Barrier Interaction by Using Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. Msphere. 2. PMID 29104935 DOI: 10.1128/mSphere.00398-17  0.8
2017 Stebbins MJ, Lippmann ES, Faubion MG, Daneman R, Palecek SP, Shusta EV. Activation of RARα, RARγ, or RXRα Increases Barrier Tightness in Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. Biotechnology Journal. PMID 28960887 DOI: 10.1002/biot.201700093  0.8
2017 Vatine GD, Al-Ahmad A, Barriga BK, Svendsen S, Salim A, Garcia L, Garcia VJ, Ho R, Yucer N, Qian T, Lim RG, Wu J, Thompson LM, Spivia WR, Chen Z, ... ... Shusta EV, et al. Modeling Psychomotor Retardation using iPSCs from MCT8-Deficient Patients Indicates a Prominent Role for the Blood-Brain Barrier. Cell Stem Cell. PMID 28526555 DOI: 10.1016/j.stem.2017.04.002  0.8
2016 Goulatis LI, Shusta EV. Protein engineering approaches for regulating blood-brain barrier transcytosis. Current Opinion in Structural Biology. 45: 109-115. PMID 28040636 DOI: 10.1016/j.sbi.2016.12.005  0.44
2016 Canfield SG, Stebbins MJ, Morales BS, Asai SW, Vatine GD, Svendsen CN, Palecek SP, Shusta EV. An Isogenic Blood-Brain Barrier Model Comprising Brain Endothelial Cells, Astrocytes and Neurons Derived from Human Induced Pluripotent Stem Cells. Journal of Neurochemistry. PMID 27935037 DOI: 10.1111/jnc.13923  0.8
2016 Wilson HK, Faubion MG, Hjortness MK, Palecek SP, Shusta EV. Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by ROCK inhibition. Tissue Engineering. Part C, Methods. PMID 27846787 DOI: 10.1089/ten.TEC.2016.0345  0.8
2016 Clark PA, Al-Ahmad AJ, Qian T, Zhang RR, Wilson HK, Weichert JP, Palecek SP, Kuo JS, Shusta EV. Analysis of Cancer-targeting Alkylphosphocholine Analog Permeability Characteristics Using a Human Induced Pluripotent Stem Cell Blood-Brain Barrier Model. Molecular Pharmaceutics. PMID 27421304 DOI: 10.1021/acs.molpharmaceut.6b00441  1
2016 Helms HC, Abbott NJ, Burek M, Cecchelli R, Couraud PO, Deli MA, Förster C, Galla HJ, Romero IA, Shusta EV, Stebbins MJ, Vandenhaute E, Weksler B, Brodin B. In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism. PMID 26868179 DOI: 10.1177/0271678X16630991  1
2015 Tillotson BJ, Goulatis LI, Parenti I, Duxbury E, Shusta EV. Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation. Plos One. 10: e0145820. PMID 26713870 DOI: 10.1371/journal.pone.0145820  1
2015 Burns ML, Malott TM, Metcalf KJ, Puguh A, Chan JR, Shusta EV. Pro-region engineering for improved yeast display and secretion of brain derived neurotrophic factor. Biotechnology Journal. PMID 26580314 DOI: 10.1002/biot.201500360  1
2015 Stebbins MJ, Wilson HK, Canfield SG, Qian T, Palecek SP, Shusta EV. Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells. Methods (San Diego, Calif.). PMID 26518252 DOI: 10.1016/j.ymeth.2015.10.016  1
2015 Qian T, Shusta EV, Palecek SP. Advances in microfluidic platforms for analyzing and regulating human pluripotent stem cells. Current Opinion in Genetics & Development. 34: 54-60. PMID 26313850 DOI: 10.1016/j.gde.2015.07.007  1
2015 Tillotson BJ, Lajoie JM, Shusta EV. Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates. Methods in Molecular Biology (Clifton, N.J.). 1319: 65-78. PMID 26060070 DOI: 10.1007/978-1-4939-2748-7_4  1
2015 Wilson HK, Canfield SG, Hjortness MK, Palecek SP, Shusta EV. Exploring the effects of cell seeding density on the differentiation of human pluripotent stem cells to brain microvascular endothelial cells. Fluids and Barriers of the Cns. 12: 13. PMID 25994964 DOI: 10.1186/s12987-015-0007-9  1
2015 Marshall CJ, Grosskopf VA, Moehling TJ, Tillotson BJ, Wiepz GJ, Abbott NL, Raines RT, Shusta EV. An evolved Mxe GyrA intein for enhanced production of fusion proteins. Acs Chemical Biology. 10: 527-38. PMID 25384269 DOI: 10.1021/cb500689g  1
2015 Lajoie JM, Shusta EV. Introducing glycophage arrays: facile production, purification and patterning of glycophages. Biotechnology Journal. 10: 20-1. PMID 25359621 DOI: 10.1002/biot.201400591  1
2015 Lajoie JM, Shusta EV. Targeting receptor-mediated transport for delivery of biologics across the blood-brain barrier. Annual Review of Pharmacology and Toxicology. 55: 613-31. PMID 25340933 DOI: 10.1146/annurev-pharmtox-010814-124852  1
2014 Lian X, Bao X, Al-Ahmad A, Liu J, Wu Y, Dong W, Dunn KK, Shusta EV, Palecek SP. Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling. Stem Cell Reports. 3: 804-16. PMID 25418725 DOI: 10.1016/j.stemcr.2014.09.005  1
2014 Wilson HK, Canfield SG, Shusta EV, Palecek SP. Concise review: tissue-specific microvascular endothelial cells derived from human pluripotent stem cells. Stem Cells (Dayton, Ohio). 32: 3037-45. PMID 25070152 DOI: 10.1002/stem.1797  1
2014 Burns ML, Malott TM, Metcalf KJ, Hackel BJ, Chan JR, Shusta EV. Directed evolution of brain-derived neurotrophic factor for improved folding and expression in Saccharomyces cerevisiae. Applied and Environmental Microbiology. 80: 5732-42. PMID 25015885 DOI: 10.1128/AEM.01466-14  1
2014 Tan LN, Wiepz GJ, Miller DS, Shusta EV, Abbott NL. Liquid crystal droplet-based amplification of microvesicles that are shed by mammalian cells. The Analyst. 139: 2386-96. PMID 24667742 DOI: 10.1039/c3an02360e  1
2014 Zhang X, Wang XX, Shusta EV. Creation and Evaluation of a Single-chain Antibody Tetramer that Targets Brain Endothelial Cells. Aiche Journal. American Institute of Chemical Engineers. 60: 1245-1252. PMID 24659822 DOI: 10.1002/aic.14348  1
2014 Jones AR, Stutz CC, Zhou Y, Marks JD, Shusta EV. Identifying blood-brain-barrier selective single-chain antibody fragments. Biotechnology Journal. 9: 664-74. PMID 24644233 DOI: 10.1002/biot.201300550  1
2014 Lippmann ES, Al-Ahmad A, Azarin SM, Palecek SP, Shusta EV. A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources. Scientific Reports. 4: 4160. PMID 24561821 DOI: 10.1038/srep04160  1
2014 Malin D, Strekalova E, Petrovic V, Deal AM, Al Ahmad A, Adamo B, Miller CR, Ugolkov A, Livasy C, Fritchie K, Hamilton E, Blackwell K, Geradts J, Ewend M, Carey L, ... Shusta EV, et al. αB-crystallin: a novel regulator of breast cancer metastasis to the brain. Clinical Cancer Research : An Official Journal of the American Association For Cancer Research. 20: 56-67. PMID 24132917 DOI: 10.1158/1078-0432.CCR-13-1255  1
2014 Stutz CC, Zhang X, Shusta EV. Combinatorial approaches for the identification of brain drug delivery targets. Current Pharmaceutical Design. 20: 1564-76. PMID 23789958 DOI: 10.2174/13816128113199990459  1
2013 Marshall CJ, Agarwal N, Kalia J, Grosskopf VA, McGrath NA, Abbott NL, Raines RT, Shusta EV. Facile chemical functionalization of proteins through intein-linked yeast display. Bioconjugate Chemistry. 24: 1634-44. PMID 23924245 DOI: 10.1021/bc4002618  1
2013 Lippmann ES, Al-Ahmad A, Palecek SP, Shusta EV. Modeling the blood-brain barrier using stem cell sources. Fluids and Barriers of the Cns. 10: 2. PMID 23305164 DOI: 10.1186/2045-8118-10-2  1
2013 Tillotson BJ, de Larrinoa IF, Skinner CA, Klavas DM, Shusta EV. Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources. Protein Engineering, Design & Selection : Peds. 26: 101-12. PMID 23109730 DOI: 10.1093/protein/gzs077  1
2013 Tillotson BJ, Cho YK, Shusta EV. Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display. Methods (San Diego, Calif.). 60: 27-37. PMID 22449570 DOI: 10.1016/j.ymeth.2012.03.010  1
2012 Pavoor TV, Wheasler JA, Kamat V, Shusta EV. An enhanced approach for engineering thermally stable proteins using yeast display. Protein Engineering, Design & Selection : Peds. 25: 625-30. PMID 22767886 DOI: 10.1093/protein/gzs041  1
2012 Lippmann ES, Azarin SM, Kay JE, Nessler RA, Wilson HK, Al-Ahmad A, Palecek SP, Shusta EV. Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells. Nature Biotechnology. 30: 783-91. PMID 22729031 DOI: 10.1038/nbt.2247  1
2011 Lippmann ES, Weidenfeller C, Svendsen CN, Shusta EV. Blood-brain barrier modeling with co-cultured neural progenitor cell-derived astrocytes and neurons. Journal of Neurochemistry. 119: 507-20. PMID 21854389 DOI: 10.1111/j.1471-4159.2011.07434.x  1
2011 Shayan G, Choi YS, Shusta EV, Shuler ML, Lee KH. Murine in vitro model of the blood-brain barrier for evaluating drug transport. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation For Pharmaceutical Sciences. 42: 148-55. PMID 21078386 DOI: 10.1016/j.ejps.2010.11.005  1
2010 Cho YK, Shusta EV. Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources. Protein Engineering, Design & Selection : Peds. 23: 567-77. PMID 20498037 DOI: 10.1093/protein/gzq029  1
2010 Agarwal N, Lippmann ES, Shusta EV. Identification and expression profiling of blood-brain barrier membrane proteins. Journal of Neurochemistry. 112: 625-35. PMID 19895664 DOI: 10.1111/j.1471-4159.2009.06481.x  1
2009 Pavoor TV, Cho YK, Shusta EV. Development of GFP-based biosensors possessing the binding properties of antibodies. Proceedings of the National Academy of Sciences of the United States of America. 106: 11895-900. PMID 19574456 DOI: 10.1073/pnas.0902828106  1
2009 Agarwal N, Shusta EV. Multiplex expression cloning of blood-brain barrier membrane proteins. Proteomics. 9: 1099-108. PMID 19180536 DOI: 10.1002/pmic.200800368  1
2009 Cho YK, Chen I, Wei X, Li L, Shusta EV. A yeast display immunoprecipitation method for efficient isolation and characterization of antigens. Journal of Immunological Methods. 341: 117-26. PMID 19041873 DOI: 10.1016/j.jim.2008.11.005  1
2009 Jones AR, Shusta EV. Antibodies and the Blood-Brain Barrier Therapeutic Monoclonal Antibodies: From Bench to Clinic. 483-502. DOI: 10.1002/9780470485408.ch21  1
2008 Huang D, Gore PR, Shusta EV. Increasing yeast secretion of heterologous proteins by regulating expression rates and post-secretory loss. Biotechnology and Bioengineering. 101: 1264-75. PMID 18781684 DOI: 10.1002/bit.22019  1
2008 Wentz AE, Shusta EV. Enhanced secretion of heterologous proteins from yeast by overexpression of ribosomal subunit RPP0. Biotechnology Progress. 24: 748-56. PMID 18396911 DOI: 10.1021/bp070345m  1
2008 Pepper LR, Cho YK, Boder ET, Shusta EV. A decade of yeast surface display technology: where are we now? Combinatorial Chemistry & High Throughput Screening. 11: 127-34. PMID 18336206 DOI: 10.2174/138620708783744516  1
2008 Calabria AR, Shusta EV. A genomic comparison of in vivo and in vitro brain microvascular endothelial cells. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism. 28: 135-48. PMID 17565361 DOI: 10.1038/sj.jcbfm.9600518  1
2007 Jones AR, Shusta EV. Blood-brain barrier transport of therapeutics via receptor-mediation. Pharmaceutical Research. 24: 1759-71. PMID 17619996 DOI: 10.1007/s11095-007-9379-0  1
2007 Weidenfeller C, Svendsen CN, Shusta EV. Differentiating embryonic neural progenitor cells induce blood-brain barrier properties. Journal of Neurochemistry. 101: 555-65. PMID 17254017 DOI: 10.1111/j.1471-4159.2006.04394.x  1
2007 Wang XX, Cho YK, Shusta EV. Mining a yeast library for brain endothelial cell-binding antibodies. Nature Methods. 4: 143-5. PMID 17206151 DOI: 10.1038/nmeth993  1
2007 Wentz AE, Shusta EV. A novel high-throughput screen reveals yeast genes that increase secretion of heterologous proteins. Applied and Environmental Microbiology. 73: 1189-98. PMID 17189442 DOI: 10.1128/AEM.02427-06  1
2007 Weidenfeller C, Shusta EV. Blood–brain barrier Endothelial Biomedicine. 1124-1139. DOI: 10.1017/CBO9780511546198.124  1
2006 Huang D, Shusta EV. A yeast platform for the production of single-chain antibody-green fluorescent protein fusions. Applied and Environmental Microbiology. 72: 7748-59. PMID 17028228 DOI: 10.1128/AEM.01403-06  1
2006 Calabria AR, Shusta EV. Blood-brain barrier genomics and proteomics: elucidating phenotype, identifying disease targets and enabling brain drug delivery. Drug Discovery Today. 11: 792-9. PMID 16935746 DOI: 10.1016/j.drudis.2006.07.006  1
2006 Calabria AR, Weidenfeller C, Jones AR, de Vries HE, Shusta EV. Puromycin-purified rat brain microvascular endothelial cell cultures exhibit improved barrier properties in response to glucocorticoid induction. Journal of Neurochemistry. 97: 922-33. PMID 16573646 DOI: 10.1111/j.1471-4159.2006.03793.x  1
2006 Hackel BJ, Huang D, Bubolz JC, Wang XX, Shusta EV. Production of soluble and active transferrin receptor-targeting single-chain antibody using Saccharomyces cerevisiae. Pharmaceutical Research. 23: 790-7. PMID 16550469 DOI: 10.1007/s11095-006-9778-7  1
2005 Wang XX, Shusta EV. The use of scFv-displaying yeast in mammalian cell surface selections. Journal of Immunological Methods. 304: 30-42. PMID 16099466 DOI: 10.1016/j.jim.2005.05.006  1
2005 Huang D, Shusta EV. Secretion and surface display of green fluorescent protein using the yeast Saccharomyces cerevisiae. Biotechnology Progress. 21: 349-57. PMID 15801770 DOI: 10.1021/bp0497482  1
2005 Shusta EV. Blood-brain barrier genomics, proteomics, and new transporter discovery. Neurorx : the Journal of the American Society For Experimental Neurotherapeutics. 2: 151-61. PMID 15717066 DOI: 10.1602/neurorx.2.1.151  1
2003 Shusta EV, Li JY, Boado RJ, Pardridge WM. The Ro52/SS-A autoantigen has elevated expression at the brain microvasculature. Neuroreport. 14: 1861-5. PMID 14534436 DOI: 10.1097/01.wnr.0000092469.09492.f9  1
2002 Shusta EV, Zhu C, Boado RJ, Pardridge WM. Subtractive expression cloning reveals high expression of CD46 at the blood-brain barrier. Journal of Neuropathology and Experimental Neurology. 61: 597-604. PMID 12125738  1
2002 Shusta EV, Boado RJ, Pardridge WM. Vascular proteomics and subtractive antibody expression cloning. Molecular & Cellular Proteomics : McP. 1: 75-82. PMID 12096143  1
2002 Shusta EV, Boado RJ, Mathern GW, Pardridge WM. Vascular genomics of the human brain. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism. 22: 245-52. PMID 11891429 DOI: 10.1097/00004647-200203000-00001  1
2001 Kieke MC, Sundberg E, Shusta EV, Mariuzza RA, Wittrup KD, Kranz DM. High affinity T cell receptors from yeast display libraries block T cell activation by superantigens. Journal of Molecular Biology. 307: 1305-15. PMID 11292343 DOI: 10.1006/jmbi.2001.4560  1
2000 Shusta EV, Holler PD, Kieke MC, Kranz DM, Wittrup KD. Directed evolution of a stable scaffold for T-cell receptor engineering. Nature Biotechnology. 18: 754-9. PMID 10888844 DOI: 10.1038/77325  1
2000 Holler PD, Holman PO, Shusta EV, O'Herrin S, Wittrup KD, Kranz DM. In vitro evolution of a T cell receptor with high affinity for peptide/MHC. Proceedings of the National Academy of Sciences of the United States of America. 97: 5387-92. PMID 10779548 DOI: 10.1073/pnas.080078297  1
1999 Shusta EV, Kieke MC, Parke E, Kranz DM, Wittrup KD. Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency. Journal of Molecular Biology. 292: 949-56. PMID 10512694 DOI: 10.1006/jmbi.1999.3130  1
1999 Kieke MC, Shusta EV, Boder ET, Teyton L, Wittrup KD, Kranz DM. Selection of functional T cell receptor mutants from a yeast surface-display library. Proceedings of the National Academy of Sciences of the United States of America. 96: 5651-6. PMID 10318939 DOI: 10.1073/pnas.96.10.5651  1
1999 Shusta EV, Vanantwerp J, Wittrup KD. Biosynthetic polypeptide libraries Current Opinion in Biotechnology. 10: 117-122. PMID 10209135 DOI: 10.1016/S0958-1669(99)80020-2  1
1998 Shusta EV, Raines RT, Plückthun A, Wittrup KD. Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments Nature Biotechnology. 16: 773-777. PMID 9702778 DOI: 10.1038/nbt0898-773  1
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