Dan Tawfik - Publications

Affiliations: 
Biological Chemistry Weizmann Institute of Science, Rehovot, Israel 
Area:
Biochemistry, Analytical Chemistry, Microbiology Biology

168 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2019 Ben-David M, Soskine M, Dubovetskyi A, Cherukuri KP, Dym O, Sussman JL, Liao Q, Szeler K, Kamerlin SCL, Tawfik DS. Enzyme neo- versus re-functionalization - an epistatic ratchet versus a smooth reversible transition. Molecular Biology and Evolution. PMID 31873734 DOI: 10.1093/molbev/msz298  0.52
2019 Despotović D, Aharon E, Dubovetskyi A, Leader H, Ashani Y, Tawfik DS. A mixture of three engineered phosphotriesterases enables rapid detoxification of the entire spectrum of known threat nerve agents. Protein Engineering, Design & Selection : Peds. PMID 31612205 DOI: 10.1093/protein/gzz039  0.52
2019 Noda-García L, Davidi D, Korenblum E, Elazar A, Putintseva E, Aharoni A, Tawfik DS. Chance and pleiotropy dominate genetic diversity in complex bacterial environments. Nature Microbiology. PMID 30936490 DOI: 10.1038/s41564-019-0412-y  0.32
2018 Trudeau DL, Edlich-Muth C, Zarzycki J, Scheffen M, Goldsmith M, Khersonsky O, Avizemer Z, Fleishman SJ, Cotton CAR, Erb TJ, Tawfik DS, Bar-Even A. Design and in vitro realization of carbon-conserving photorespiration. Proceedings of the National Academy of Sciences of the United States of America. PMID 30459276 DOI: 10.1073/pnas.1812605115  0.32
2018 Khersonsky O, Lipsh R, Avizemer Z, Ashani Y, Goldsmith M, Leader H, Dym O, Rogotner S, Trudeau DL, Prilusky J, Amengual-Rigo P, Guallar V, Tawfik DS, Fleishman SJ. Automated Design of Efficient and Functionally Diverse Enzyme Repertoires. Molecular Cell. PMID 30270109 DOI: 10.1016/j.molcel.2018.08.033  0.52
2018 Kaltenbach M, Burke JR, Dindo M, Pabis A, Munsberg FS, Rabin A, Kamerlin SCL, Noel JP, Tawfik DS. Publisher Correction: Evolution of chalcone isomerase from a noncatalytic ancestor. Nature Chemical Biology. PMID 29760514 DOI: 10.1038/s41589-018-0079-3  0.52
2018 Kaltenbach M, Burke JR, Dindo M, Pabis A, Munsberg FS, Rabin A, Kamerlin SCL, Noel JP, Tawfik DS. Evolution of chalcone isomerase from a noncatalytic ancestor. Nature Chemical Biology. PMID 29686356 DOI: 10.1038/s41589-018-0042-3  0.52
2018 Goldenzweig A, Goldsmith M, Hill SE, Gertman O, Laurino P, Ashani Y, Dym O, Unger T, Albeck S, Prilusky J, Lieberman RL, Aharoni A, Silman I, Sussman JL, Tawfik DS, et al. Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability. Molecular Cell. 70: 380. PMID 29677494 DOI: 10.1016/j.molcel.2018.03.035  0.52
2018 Lei L, Cherukuri KP, Alcolombri U, Meltzer D, Tawfik DS. The DMSP Lyase and Lyase-Like Cupin family consists of bona fide DMSP lyases as well as other enzymes with unknown function. Biochemistry. PMID 29561599 DOI: 10.1021/acs.biochem.8b00097  0.52
2017 Goldsmith M, Aggarwal N, Ashani Y, Jubran H, Greisen PJ, Ovchinnikov S, Leader H, Baker D, Sussman JL, Goldenzweig A, Fleishman SJ, Tawfik DS. Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers. Protein Engineering, Design & Selection : Peds. 1-13. PMID 28159998 DOI: 10.1093/protein/gzx003  0.52
2016 Romero Romero ML, Rabin A, Tawfik DS. Functional Proteins from Short Peptides: Dayhoff's Hypothesis Turns 50. Angewandte Chemie (International Ed. in English). PMID 27865046 DOI: 10.1002/anie.201609977  0.4
2016 Goldenzweig A, Goldsmith M, Hill SE, Gertman O, Laurino P, Ashani Y, Dym O, Unger T, Albeck S, Prilusky J, Lieberman RL, Aharoni A, Silman I, Sussman JL, Tawfik DS, et al. Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability. Molecular Cell. PMID 27425410 DOI: 10.1016/j.molcel.2016.06.012  1
2016 Trudeau DL, Kaltenbach M, Tawfik DS. On the potential origins of the high stability of reconstructed ancestral proteins. Molecular Biology and Evolution. PMID 27413048 DOI: 10.1093/molbev/msw138  0.4
2016 Wille T, Neumaier K, Koller M, Ehinger C, Aggarwal N, Ashani Y, Goldsmith M, Sussman JL, Tawfik DS, Thiermann H, Worek F. Single treatment of VX poisoned guinea pigs with the phosphotriesterase mutant C23AL: Intraosseous versus intravenous injection. Toxicology Letters. PMID 27397758 DOI: 10.1016/j.toxlet.2016.07.004  1
2016 Goldsmith M, Ashani Y, Margalit R, Nyska A, Mirelman D, Tawfik DS. A new post-intoxication treatment of paraoxon and parathion poisonings using an evolved PON1 variant and recombinant GOT1. Chemico-Biological Interactions. PMID 27256520 DOI: 10.1016/j.cbi.2016.05.034  1
2016 Bolon DN, Baker D, Tawfik DS. Editorial. Protein Science : a Publication of the Protein Society. PMID 27214768 DOI: 10.1002/pro.2949  1
2016 Sarkisyan KS, Bolotin DA, Meer MV, Usmanova DR, Mishin AS, Sharonov GV, Ivankov DN, Bozhanova NG, Baranov MS, Soylemez O, Bogatyreva NS, Vlasov PK, Egorov ES, Logacheva MD, Kondrashov AS, ... ... Tawfik DS, et al. Local fitness landscape of the green fluorescent protein. Nature. 533: 397-401. PMID 27193686 DOI: 10.1038/nature17995  1
2016 Boucher JI, Bolon DN, Tawfik DS. Quantifying and understanding the fitness effects of protein mutations: Laboratory versus nature. Protein Science : a Publication of the Protein Society. PMID 27010590 DOI: 10.1002/pro.2928  1
2016 Tawfik DS, van der Donk WA. Editorial overview: Biocatalysis and Biotransformation: Esoteric, Niche Enzymology. Current Opinion in Chemical Biology. PMID 26972779 DOI: 10.1016/j.cbpa.2016.03.002  1
2016 Laurino P, Tóth-Petróczy Á, Meana-Pañeda R, Lin W, Truhlar DG, Tawfik DS. An Ancient Fingerprint Indicates the Common Ancestry of Rossmann-Fold Enzymes Utilizing Different Ribose-Based Cofactors. Plos Biology. 14: e1002396. PMID 26938925 DOI: 10.1371/journal.pbio.1002396  1
2016 Smock RG, Yadid I, Dym O, Clarke J, Tawfik DS. De Novo Evolutionary Emergence of a Symmetrical Protein Is Shaped by Folding Constraints. Cell. PMID 26806127 DOI: 10.1016/j.cell.2015.12.024  1
2016 Lavy T, Yanagida H, Tawfik DS. Gal3 Binds Gal80 Tighter than Gal1 Indicating Adaptive Protein Changes Following Duplication. Molecular Biology and Evolution. 33: 472-7. PMID 26516093 DOI: 10.1093/molbev/msv240  1
2015 Goldsmith M, Eckstein S, Ashani Y, Greisen P, Leader H, Sussman JL, Aggarwal N, Ovchinnikov S, Tawfik DS, Baker D, Thiermann H, Worek F. Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro. Archives of Toxicology. PMID 26612364 DOI: 10.1007/s00204-015-1626-2  1
2015 Bar-Rogovsky H, Stern A, Penn O, Kobl I, Pupko T, Tawfik DS. Assessing the prediction fidelity of ancestral reconstruction by a library approach. Protein Engineering, Design & Selection : Peds. 28: 507-18. PMID 26275856 DOI: 10.1093/protein/gzv038  1
2015 Rockah-Shmuel L, Tóth-Petróczy Á, Tawfik DS. Systematic Mapping of Protein Mutational Space by Prolonged Drift Reveals the Deleterious Effects of Seemingly Neutral Mutations. Plos Computational Biology. 11: e1004421. PMID 26274323 DOI: 10.1371/journal.pcbi.1004421  1
2015 Yanagida H, Gispan A, Kadouri N, Rozen S, Sharon M, Barkai N, Tawfik DS. The Evolutionary Potential of Phenotypic Mutations. Plos Genetics. 11: e1005445. PMID 26244544 DOI: 10.1371/journal.pgen.1005445  1
2015 Bar-Even A, Milo R, Noor E, Tawfik DS. The Moderately Efficient Enzyme: Futile Encounters and Enzyme Floppiness. Biochemistry. 54: 4969-77. PMID 26219075 DOI: 10.1021/acs.biochem.5b00621  1
2015 Alcolombri U, Ben-Dor S, Feldmesser E, Levin Y, Tawfik DS, Vardi A. MARINE SULFUR CYCLE. Identification of the algal dimethyl sulfide-releasing enzyme: A missing link in the marine sulfur cycle. Science (New York, N.Y.). 348: 1466-9. PMID 26113722 DOI: 10.1126/science.aab1586  1
2015 Dellus-Gur E, Elias M, Caselli E, Prati F, Salverda ML, de Visser JA, Fraser JS, Tawfik DS. Negative Epistasis and Evolvability in TEM-1 β-Lactamase-The Thin Line between an Enzyme's Conformational Freedom and Disorder. Journal of Molecular Biology. 427: 2396-409. PMID 26004540 DOI: 10.1016/j.jmb.2015.05.011  1
2015 Ben-David M, Sussman JL, Maxwell CI, Szeler K, Kamerlin SC, Tawfik DS. Catalytic stimulation by restrained active-site floppiness--the case of high density lipoprotein-bound serum paraoxonase-1. Journal of Molecular Biology. 427: 1359-74. PMID 25644661 DOI: 10.1016/j.jmb.2015.01.013  1
2014 Warszawski S, Netzer R, Tawfik DS, Fleishman SJ. A "fuzzy"-logic language for encoding multiple physical traits in biomolecules. Journal of Molecular Biology. 426: 4125-38. PMID 25311857 DOI: 10.1016/j.jmb.2014.10.002  1
2014 Worek F, Seeger T, Reiter G, Goldsmith M, Ashani Y, Leader H, Sussman JL, Aggarwal N, Thiermann H, Tawfik DS. Post-exposure treatment of VX poisoned guinea pigs with the engineered phosphotriesterase mutant C23: a proof-of-concept study. Toxicology Letters. 231: 45-54. PMID 25195526 DOI: 10.1016/j.toxlet.2014.09.003  1
2014 Fanning SW, Walter R, Horn JR, Tawfik D. Structural basis of an engineered dual-specific antibody: Conformational diversity leads to a hypervariable loop metal-binding site Protein Engineering, Design and Selection. 27: 391-397. PMID 25143596 DOI: 10.1093/protein/gzu033  1
2014 Alcolombri U, Laurino P, Lara-Astiaso P, Vardi A, Tawfik DS. DddD is a CoA-transferase/lyase producing dimethyl sulfide in the marine environment. Biochemistry. 53: 5473-5. PMID 25140443 DOI: 10.1021/bi500853s  1
2014 Rockah-Shmuel L, Tawfik DS, Goldsmith M. Generating targeted libraries by the combinatorial incorporation of synthetic oligonucleotides during gene shuffling (ISOR). Methods in Molecular Biology (Clifton, N.J.). 1179: 129-37. PMID 25055774 DOI: 10.1007/978-1-4939-1053-3_8  1
2014 Tóth-Petróczy A, Tawfik DS. The robustness and innovability of protein folds. Current Opinion in Structural Biology. 26: 131-8. PMID 25038399 DOI: 10.1016/j.sbi.2014.06.007  1
2014 Tawfik DS. Accuracy-rate tradeoffs: how do enzymes meet demands of selectivity and catalytic efficiency? Current Opinion in Chemical Biology. 21: 73-80. PMID 24954689 DOI: 10.1016/j.cbpa.2014.05.008  1
2014 Tóth-Petróczy A, Tawfik DS. Hopeful (protein InDel) monsters? Structure (London, England : 1993). 22: 803-4. PMID 24918338 DOI: 10.1016/j.str.2014.05.013  1
2014 Alcolombri U, Elias M, Vardi A, Tawfik DS. Ambiguous evidence for assigning DddQ as a dimethylsulfoniopropionate lyase and oceanic dimethylsulfide producer. Proceedings of the National Academy of Sciences of the United States of America. 111: E2078-9. PMID 24760823 DOI: 10.1073/pnas.1401685111  1
2014 Worek F, Seeger T, Goldsmith M, Ashani Y, Leader H, Sussman JS, Tawfik D, Thiermann H, Wille T. Efficacy of the rePON1 mutant IIG1 to prevent cyclosarin toxicity in vivo and to detoxify structurally different nerve agents in vitro. Archives of Toxicology. 88: 1257-66. PMID 24477626 DOI: 10.1007/s00204-014-1204-z  1
2014 Elias M, Wieczorek G, Rosenne S, Tawfik DS. The universality of enzymatic rate-temperature dependency. Trends in Biochemical Sciences. 39: 1-7. PMID 24315123 DOI: 10.1016/j.tibs.2013.11.001  1
2013 Rockah-Shmuel L, Tóth-Petróczy Á, Sela A, Wurtzel O, Sorek R, Tawfik DS. Correlated occurrence and bypass of frame-shifting insertion-deletions (InDels) to give functional proteins. Plos Genetics. 9: e1003882. PMID 24204297 DOI: 10.1371/journal.pgen.1003882  1
2013 Cherny I, Greisen P, Ashani Y, Khare SD, Oberdorfer G, Leader H, Baker D, Tawfik DS. Engineering V-type nerve agents detoxifying enzymes using computationally focused libraries. Acs Chemical Biology. 8: 2394-403. PMID 24041203 DOI: 10.1021/cb4004892  1
2013 Wellner A, Raitses Gurevich M, Tawfik DS. Mechanisms of protein sequence divergence and incompatibility. Plos Genetics. 9: e1003665. PMID 23935519 DOI: 10.1371/journal.pgen.1003665  1
2013 Bar-Rogovsky H, Hugenmatter A, Tawfik DS. The evolutionary origins of detoxifying enzymes: the mammalian serum paraoxonases (PONs) relate to bacterial homoserine lactonases. The Journal of Biological Chemistry. 288: 23914-27. PMID 23788644 DOI: 10.1074/jbc.M112.427922  1
2013 Dellus-Gur E, Toth-Petroczy A, Elias M, Tawfik DS. What makes a protein fold amenable to functional innovation? Fold polarity and stability trade-offs. Journal of Molecular Biology. 425: 2609-21. PMID 23542341 DOI: 10.1016/j.jmb.2013.03.033  1
2013 Goldsmith M, Tawfik DS. Enzyme engineering by targeted libraries. Methods in Enzymology. 523: 257-83. PMID 23422434 DOI: 10.1016/B978-0-12-394292-0.00012-6  1
2013 Ben-David M, Wieczorek G, Elias M, Silman I, Sussman JL, Tawfik DS. Catalytic metal ion rearrangements underline promiscuity and evolvability of a metalloenzyme. Journal of Molecular Biology. 425: 1028-38. PMID 23318950 DOI: 10.1016/j.jmb.2013.01.009  1
2013 Tóth-Petróczy A, Tawfik DS. Protein insertions and deletions enabled by neutral roaming in sequence space. Molecular Biology and Evolution. 30: 761-71. PMID 23315956 DOI: 10.1093/molbev/mst003  1
2012 Tokuriki N, Jackson CJ, Afriat-Jurnou L, Wyganowski KT, Tang R, Tawfik DS. Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme. Nature Communications. 3: 1257. PMID 23212386 DOI: 10.1038/ncomms2246  1
2012 Rockah-Shmuel L, Tawfik DS. Evolutionary transitions to new DNA methyltransferases through target site expansion and shrinkage. Nucleic Acids Research. 40: 11627-37. PMID 23074188 DOI: 10.1093/nar/gks944  1
2012 Elias M, Wellner A, Goldin-Azulay K, Chabriere E, Vorholt JA, Erb TJ, Tawfik DS. The molecular basis of phosphate discrimination in arsenate-rich environments. Nature. 491: 134-7. PMID 23034649 DOI: 10.1038/nature11517  1
2012 Hornung G, Bar-Ziv R, Rosin D, Tokuriki N, Tawfik DS, Oren M, Barkai N. Noise-mean relationship in mutated promoters. Genome Research. 22: 2409-17. PMID 22820945 DOI: 10.1101/gr.139378.112  1
2012 Afriat-Jurnou L, Jackson CJ, Tawfik DS. Reconstructing a missing link in the evolution of a recently diverged phosphotriesterase by active-site loop remodeling. Biochemistry. 51: 6047-55. PMID 22809311 DOI: 10.1021/bi300694t  1
2012 Khersonsky O, Kiss G, Röthlisberger D, Dym O, Albeck S, Houk KN, Baker D, Tawfik DS. Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59. Proceedings of the National Academy of Sciences of the United States of America. 109: 10358-63. PMID 22685214 DOI: 10.1073/pnas.1121063109  1
2012 Kipnis Y, Dellus-Gur E, Tawfik DS. TRINS: a method for gene modification by randomized tandem repeat insertions. Protein Engineering, Design & Selection : Peds. 25: 437-44. PMID 22670060 DOI: 10.1093/protein/gzs023  1
2012 Goldsmith M, Tawfik DS. Directed enzyme evolution: beyond the low-hanging fruit. Current Opinion in Structural Biology. 22: 406-12. PMID 22579412 DOI: 10.1016/j.sbi.2012.03.010  1
2012 Goldsmith M, Ashani Y, Simo Y, Ben-David M, Leader H, Silman I, Sussman JL, Tawfik DS. Evolved stereoselective hydrolases for broad-spectrum G-type nerve agent detoxification. Chemistry & Biology. 19: 456-66. PMID 22520752 DOI: 10.1016/j.chembiol.2012.01.017  1
2012 Ben-David M, Elias M, Filippi JJ, Duñach E, Silman I, Sussman JL, Tawfik DS. Catalytic versatility and backups in enzyme active sites: the case of serum paraoxonase 1. Journal of Molecular Biology. 418: 181-96. PMID 22387469 DOI: 10.1016/j.jmb.2012.02.042  1
2012 Khare SD, Kipnis Y, Greisen P, Takeuchi R, Ashani Y, Goldsmith M, Song Y, Gallaher JL, Silman I, Leader H, Sussman JL, Stoddard BL, Tawfik DS, Baker D. Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis. Nature Chemical Biology. 8: 294-300. PMID 22306579 DOI: 10.1038/nchembio.777  1
2012 Elias M, Tawfik DS. Divergence and convergence in enzyme evolution: parallel evolution of paraoxonases from quorum-quenching lactonases. The Journal of Biological Chemistry. 287: 11-20. PMID 22069329 DOI: 10.1074/jbc.R111.257329  1
2011 Camps J, Joven J, Mackness B, Mackness M, Tawfik D, Draganov D, Costa LG, Paragh G, Seres I, Horke S, James R, Hernández A, Reddy S, Shih D, Navab M, et al. Paraoxonase-1 and clopidogrel efficacy. Nature Medicine. 17: 1041-2; author reply. PMID 21900915 DOI: 10.1038/nm.2386  1
2011 Ashani Y, Goldsmith M, Leader H, Silman I, Sussman JL, Tawfik DS. In vitro detoxification of cyclosarin in human blood pre-incubated ex vivo with recombinant serum paraoxonases. Toxicology Letters. 206: 24-8. PMID 21807078 DOI: 10.1016/j.toxlet.2011.07.017  1
2011 Alcolombri U, Elias M, Tawfik DS. Directed evolution of sulfotransferases and paraoxonases by ancestral libraries. Journal of Molecular Biology. 411: 837-53. PMID 21723874 DOI: 10.1016/j.jmb.2011.06.037  1
2011 Tóth-Petróczy A, Tawfik DS. Slow protein evolutionary rates are dictated by surface-core association. Proceedings of the National Academy of Sciences of the United States of America. 108: 11151-6. PMID 21690394 DOI: 10.1073/pnas.1015994108  1
2011 Bar-Even A, Noor E, Savir Y, Liebermeister W, Davidi D, Tawfik DS, Milo R. The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters. Biochemistry. 50: 4402-10. PMID 21506553 DOI: 10.1021/bi2002289  1
2011 Salverda ML, Dellus E, Gorter FA, Debets AJ, van der Oost J, Hoekstra RF, Tawfik DS, de Visser JA. Initial mutations direct alternative pathways of protein evolution. Plos Genetics. 7: e1001321. PMID 21408208 DOI: 10.1371/journal.pgen.1001321  1
2011 Khersonsky O, Malitsky S, Rogachev I, Tawfik DS. Role of chemistry versus substrate binding in recruiting promiscuous enzyme functions. Biochemistry. 50: 2683-90. PMID 21332126 DOI: 10.1021/bi101763c  1
2011 Khersonsky O, Röthlisberger D, Wollacott AM, Murphy P, Dym O, Albeck S, Kiss G, Houk KN, Baker D, Tawfik DS. Optimization of the in-silico-designed kemp eliminase KE70 by computational design and directed evolution. Journal of Molecular Biology. 407: 391-412. PMID 21277311 DOI: 10.1016/j.jmb.2011.01.041  1
2011 Gupta RD, Goldsmith M, Ashani Y, Simo Y, Mullokandov G, Bar H, Ben-David M, Leader H, Margalit R, Silman I, Sussman JL, Tawfik DS. Directed evolution of hydrolases for prevention of G-type nerve agent intoxication. Nature Chemical Biology. 7: 120-5. PMID 21217689 DOI: 10.1038/nchembio.510  1
2011 Tawfik DS, Viola RE. Arsenate replacing phosphate: alternative life chemistries and ion promiscuity. Biochemistry. 50: 1128-34. PMID 21214261 DOI: 10.1021/bi200002a  1
2011 Yadid I, Tawfik DS. Functional β-propeller lectins by tandem duplications of repetitive units. Protein Engineering, Design & Selection : Peds. 24: 185-95. PMID 20713410 DOI: 10.1093/protein/gzq053  1
2011 Aharoni A, Tawfik DS. In Vitro Compartmentalization (IVC) and Other High-Throughput Screens of Enzyme Libraries Protein Engineering Handbook, Volume 1 &Amp; Volume 2. 2: 649-667. DOI: 10.1002/9783527634026.ch27  1
2010 Tawfik DS. Messy biology and the origins of evolutionary innovations. Nature Chemical Biology. 6: 692-6. PMID 20852602 DOI: 10.1038/nchembio.441  1
2010 Soskine M, Tawfik DS. Mutational effects and the evolution of new protein functions. Nature Reviews. Genetics. 11: 572-82. PMID 20634811 DOI: 10.1038/nrg2808  1
2010 Yadid I, Kirshenbaum N, Sharon M, Dym O, Tawfik DS. Metamorphic proteins mediate evolutionary transitions of structure. Proceedings of the National Academy of Sciences of the United States of America. 107: 7287-92. PMID 20368465 DOI: 10.1073/pnas.0912616107  1
2010 Ashani Y, Gupta RD, Goldsmith M, Silman I, Sussman JL, Tawfik DS, Leader H. Stereo-specific synthesis of analogs of nerve agents and their utilization for selection and characterization of paraoxonase (PON1) catalytic scavengers. Chemico-Biological Interactions. 187: 362-9. PMID 20303930 DOI: 10.1016/j.cbi.2010.02.039  1
2010 Khersonsky O, Tawfik DS. Enzyme promiscuity: a mechanistic and evolutionary perspective. Annual Review of Biochemistry. 79: 471-505. PMID 20235827 DOI: 10.1146/annurev-biochem-030409-143718  1
2010 Khersonsky O, Röthlisberger D, Dym O, Albeck S, Jackson CJ, Baker D, Tawfik DS. Evolutionary optimization of computationally designed enzymes: Kemp eliminases of the KE07 series. Journal of Molecular Biology. 396: 1025-42. PMID 20036254 DOI: 10.1016/j.jmb.2009.12.031  1
2010 Gaidukov L, Viji RI, Yacobson S, Rosenblat M, Aviram M, Tawfik DS. ApoE induces serum paraoxonase PON1 activity and stability similar to ApoA-I. Biochemistry. 49: 532-8. PMID 20025294 DOI: 10.1021/bi9013227  1
2010 Khersonsky O, Tawfik DS. Enzyme promiscuity - evolutionary and mechanistic aspects Comprehensive Natural Products Ii: Chemistry and Biology. 8: 47-88.  1
2009 Jackson CJ, Foo JL, Tokuriki N, Afriat L, Carr PD, Kim HK, Schenk G, Tawfik DS, Ollis DL. Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase. Proceedings of the National Academy of Sciences of the United States of America. 106: 21631-6. PMID 19966226 DOI: 10.1073/pnas.0907548106  1
2009 Gaidukov L, Bar D, Yacobson S, Naftali E, Kaufman O, Tabakman R, Tawfik DS, Levy-Nissenbaum E. In vivo administration of BL-3050: highly stable engineered PON1-HDL complexes. Bmc Clinical Pharmacology. 9: 18. PMID 19922610 DOI: 10.1186/1472-6904-9-18  1
2009 Wood KV, Tawfik DS. Protein technologies. Current Opinion in Biotechnology. 20: 381-2. PMID 19783134 DOI: 10.1016/j.copbio.2009.09.001  1
2009 Tokuriki N, Tawfik DS. Stability effects of mutations and protein evolvability. Current Opinion in Structural Biology. 19: 596-604. PMID 19765975 DOI: 10.1016/j.sbi.2009.08.003  1
2009 Levin KB, Dym O, Albeck S, Magdassi S, Keeble AH, Kleanthous C, Tawfik DS. Following evolutionary paths to protein-protein interactions with high affinity and selectivity. Nature Structural & Molecular Biology. 16: 1049-55. PMID 19749752 DOI: 10.1038/nsmb.1670  1
2009 Tokuriki N, Tawfik DS. Chaperonin overexpression promotes genetic variation and enzyme evolution. Nature. 459: 668-73. PMID 19494908 DOI: 10.1038/nature08009  1
2009 Khersonsky O, Rosenblat M, Toker L, Yacobson S, Hugenmatter A, Silman I, Sussman JL, Aviram M, Tawfik DS. Directed evolution of serum paraoxonase PON3 by family shuffling and ancestor/consensus mutagenesis, and its biochemical characterization Biochemistry. 48: 6644-6654. PMID 19492856 DOI: 10.1021/bi900583y  1
2009 Tokuriki N, Tawfik DS. Protein dynamism and evolvability. Science (New York, N.Y.). 324: 203-7. PMID 19359577 DOI: 10.1126/science.1169375  1
2009 Goldsmith M, Tawfik DS. Potential role of phenotypic mutations in the evolution of protein expression and stability. Proceedings of the National Academy of Sciences of the United States of America. 106: 6197-202. PMID 19339491 DOI: 10.1073/pnas.0809506106  1
2009 Tokuriki N, Oldfield CJ, Uversky VN, Berezovsky IN, Tawfik DS. Do viral proteins possess unique biophysical features? Trends in Biochemical Sciences. 34: 53-9. PMID 19062293 DOI: 10.1016/j.tibs.2008.10.009  1
2008 Gupta RD, Tawfik DS. Directed enzyme evolution via small and effective neutral drift libraries. Nature Methods. 5: 939-42. PMID 18931667 DOI: 10.1038/nmeth.1262  1
2008 Bershtein S, Tawfik DS. Ohno's model revisited: measuring the frequency of potentially adaptive mutations under various mutational drifts. Molecular Biology and Evolution. 25: 2311-8. PMID 18687656 DOI: 10.1093/molbev/msn174  1
2008 Bershtein S, Goldin K, Tawfik DS. Intense neutral drifts yield robust and evolvable consensus proteins. Journal of Molecular Biology. 379: 1029-44. PMID 18495157 DOI: 10.1016/j.jmb.2008.04.024  1
2008 Tokuriki N, Stricher F, Serrano L, Tawfik DS. How protein stability and new functions trade off. Plos Computational Biology. 4: e1000002. PMID 18463696 DOI: 10.1371/journal.pcbi.1000002  1
2008 Röthlisberger D, Khersonsky O, Wollacott AM, Jiang L, DeChancie J, Betker J, Gallaher JL, Althoff EA, Zanghellini A, Dym O, Albeck S, Houk KN, Tawfik DS, Baker D. Kemp elimination catalysts by computational enzyme design. Nature. 453: 190-5. PMID 18354394 DOI: 10.1038/nature06879  1
2008 Bershtein S, Tawfik DS. Advances in laboratory evolution of enzymes. Current Opinion in Chemical Biology. 12: 151-8. PMID 18284924 DOI: 10.1016/j.cbpa.2008.01.027  1
2007 Peisajovich SG, Tawfik DS. Protein engineers turned evolutionists. Nature Methods. 4: 991-4. PMID 18049465 DOI: 10.1038/nmeth1207-991  1
2007 Goldsmith M, Kiss C, Bradbury AR, Tawfik DS. Avoiding and controlling double transformation artifacts. Protein Engineering, Design & Selection : Peds. 20: 315-8. PMID 17575283 DOI: 10.1093/protein/gzm026  1
2007 Herman A, Tawfik DS. Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries. Protein Engineering, Design & Selection : Peds. 20: 219-26. PMID 17483523 DOI: 10.1093/protein/gzm014  1
2007 Tokuriki N, Stricher F, Schymkowitz J, Serrano L, Tawfik DS. The stability effects of protein mutations appear to be universally distributed. Journal of Molecular Biology. 369: 1318-32. PMID 17482644 DOI: 10.1016/j.jmb.2007.03.069  1
2007 Gaidukov L, Tawfik DS. The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activity. Journal of Lipid Research. 48: 1637-46. PMID 17435182 DOI: 10.1194/jlr.D600045-JLR200  1
2007 Yadid I, Tawfik DS. Reconstruction of functional beta-propeller lectins via homo-oligomeric assembly of shorter fragments. Journal of Molecular Biology. 365: 10-7. PMID 17054983 DOI: 10.1016/j.jmb.2006.09.055  1
2006 Bershtein S, Segal M, Bekerman R, Tokuriki N, Tawfik DS. Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein. Nature. 444: 929-32. PMID 17122770 DOI: 10.1038/nature05385  1
2006 Afriat L, Roodveldt C, Manco G, Tawfik DS. The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase. Biochemistry. 45: 13677-86. PMID 17105187 DOI: 10.1021/bi061268r  1
2006 Khersonsky O, Roodveldt C, Tawfik DS. Enzyme promiscuity: evolutionary and mechanistic aspects. Current Opinion in Chemical Biology. 10: 498-508. PMID 16939713 DOI: 10.1016/j.cbpa.2006.08.011  1
2006 Gaidukov L, Rosenblat M, Aviram M, Tawfik DS. The 192R/Q polymorphs of serum paraoxonase PON1 differ in HDL binding, lipolactonase stimulation, and cholesterol efflux. Journal of Lipid Research. 47: 2492-502. PMID 16914770 DOI: 10.1194/jlr.M600297-JLR200  1
2006 Griffiths AD, Tawfik DS. Miniaturising the laboratory in emulsion droplets. Trends in Biotechnology. 24: 395-402. PMID 16843558 DOI: 10.1016/j.tibtech.2006.06.009  1
2006 Miller OJ, Bernath K, Agresti JJ, Amitai G, Kelly BT, Mastrobattista E, Taly V, Magdassi S, Tawfik DS, Griffiths AD. Directed evolution by in vitro compartmentalization. Nature Methods. 3: 561-70. PMID 16791215 DOI: 10.1038/nmeth897  1
2006 Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD. Amplification of complex gene libraries by emulsion PCR. Nature Methods. 3: 545-50. PMID 16791213 DOI: 10.1038/nmeth896  1
2006 Amitai G, Gaidukov L, Adani R, Yishay S, Yacov G, Kushnir M, Teitlboim S, Lindenbaum M, Bel P, Khersonsky O, Tawfik DS, Meshulam H. Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase. The Febs Journal. 273: 1906-19. PMID 16640555 DOI: 10.1111/j.1742-4658.2006.05198.x  1
2006 Garel J, Tawfik DS. Mechanism of hydrolysis and aminolysis of homocysteine thiolactone. Chemistry (Weinheim An Der Bergstrasse, Germany). 12: 4144-52. PMID 16453352 DOI: 10.1002/chem.200501145  1
2006 Tawfik DS. Biochemistry. Loop grafting and the origins of enzyme species. Science (New York, N.Y.). 311: 475-6. PMID 16439649 DOI: 10.1126/science.1123883  1
2006 Peisajovich SG, Rockah L, Tawfik DS. Evolution of new protein topologies through multistep gene rearrangements. Nature Genetics. 38: 168-74. PMID 16415885 DOI: 10.1038/ng1717  1
2006 Khersonsky O, Tawfik DS. The histidine 115-histidine 134 dyad mediates the lactonase activity of mammalian serum paraoxonases. The Journal of Biological Chemistry. 281: 7649-56. PMID 16407305 DOI: 10.1074/jbc.M512594200  1
2006 Rosenblat M, Gaidukov L, Khersonsky O, Vaya J, Oren R, Tawfik DS, Aviram M. The catalytic histidine dyad of high density lipoprotein-associated serum paraoxonase-1 (PON1) is essential for PON1-mediated inhibition of low density lipoprotein oxidation and stimulation of macrophage cholesterol efflux. The Journal of Biological Chemistry. 281: 7657-65. PMID 16407304 DOI: 10.1074/jbc.M512595200  1
2006 Khersonsky O, Tawfik DS. Chromogenic and fluorogenic assays for the lactonase activity of serum paraoxonases. Chembiochem : a European Journal of Chemical Biology. 7: 49-53. PMID 16329153 DOI: 10.1002/cbic.200500334  1
2005 Aharoni A, Amitai G, Bernath K, Magdassi S, Tawfik DS. High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. Chemistry & Biology. 12: 1281-9. PMID 16356845 DOI: 10.1016/j.chembiol.2005.09.012  1
2005 Roodveldt C, Tawfik DS. Shared promiscuous activities and evolutionary features in various members of the amidohydrolase superfamily. Biochemistry. 44: 12728-36. PMID 16171387 DOI: 10.1021/bi051021e  1
2005 James LC, Tawfik DS. Structure and kinetics of a transient antibody binding intermediate reveal a kinetic discrimination mechanism in antigen recognition. Proceedings of the National Academy of Sciences of the United States of America. 102: 12730-5. PMID 16129832 DOI: 10.1073/pnas.0500909102  1
2005 Gaidukov L, Tawfik DS. High affinity, stability, and lactonase activity of serum paraoxonase PON1 anchored on HDL with ApoA-I. Biochemistry. 44: 11843-54. PMID 16128586 DOI: 10.1021/bi050862i  1
2005 Fernández A, Tawfik DS, Berkhout B, Sanders R, Kloczkowski A, Sen T, Jernigan B. Protein promiscuity: drug resistance and native functions--HIV-1 case. Journal of Biomolecular Structure & Dynamics. 22: 615-24. PMID 15842167 DOI: 10.1080/07391102.2005.10531228  1
2005 Khersonsky O, Tawfik DS. Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase. Biochemistry. 44: 6371-82. PMID 15835926 DOI: 10.1021/bi047440d  1
2005 Aharoni A, Griffiths AD, Tawfik DS. High-throughput screens and selections of enzyme-encoding genes. Current Opinion in Chemical Biology. 9: 210-6. PMID 15811807 DOI: 10.1016/j.cbpa.2005.02.002  1
2005 Gould SM, Tawfik DS. Directed evolution of the promiscuous esterase activity of carbonic anhydrase II. Biochemistry. 44: 5444-52. PMID 15807537 DOI: 10.1021/bi0475471  1
2005 Roodveldt C, Tawfik DS. Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state Protein Engineering, Design and Selection. 18: 51-58. PMID 15790580 DOI: 10.1093/protein/gzi005  1
2005 Roodveldt C, Aharoni A, Tawfik DS. Directed evolution of proteins for heterologous expression and stability. Current Opinion in Structural Biology. 15: 50-6. PMID 15718133 DOI: 10.1016/j.sbi.2005.01.001  1
2005 Bernath K, Magdassi S, Tawfik DS. Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery. Journal of Molecular Biology. 345: 1015-26. PMID 15644201 DOI: 10.1016/j.jmb.2004.11.017  1
2005 Cohen HM, Griffiths AD, Tawfik DS, Loakes D. Determinants of cofactor binding to DNA methyltransferases: insights from a systematic series of structural variants of S-adenosylhomocysteine. Organic & Biomolecular Chemistry. 3: 152-61. PMID 15602611 DOI: 10.1039/b415446k  1
2005 Aharoni A, Gaidukov L, Khersonsky O, McQ Gould S, Roodveldt C, Tawfik DS. The 'evolvability' of promiscuous protein functions. Nature Genetics. 37: 73-6. PMID 15568024 DOI: 10.1038/ng1482  1
2004 Aharoni A, Tawfik DS. Directed evolution of recombinant serum paraoxonase (PON) variants. Discovery Medicine. 4: 120-4. PMID 20705007  0.32
2004 Hai M, Bernath K, Tawfik D, Magdassi S. Flow cytometry: a new method to investigate the properties of water-in-oil-in-water emulsions. Langmuir : the Acs Journal of Surfaces and Colloids. 20: 2081-5. PMID 15835655 DOI: 10.1021/la035402+  1
2004 Harel M, Aharoni A, Gaidukov L, Brumshtein B, Khersonsky O, Meged R, Dvir H, Ravelli RB, McCarthy A, Toker L, Silman I, Sussman JL, Tawfik DS. Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes. Nature Structural & Molecular Biology. 11: 412-9. PMID 15098021 DOI: 10.1038/nsmb767  1
2004 Cohen HM, Tawfik DS, Griffiths AD. Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization. Protein Engineering, Design & Selection : Peds. 17: 3-11. PMID 14985532 DOI: 10.1093/protein/gzh001  1
2004 Bernath K, Hai M, Mastrobattista E, Griffiths AD, Magdassi S, Tawfik DS. In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting. Analytical Biochemistry. 325: 151-7. PMID 14715296 DOI: 10.1016/j.ab.2003.10.005  1
2004 Aharoni A, Gaidukov L, Yagur S, Toker L, Silman I, Tawfik DS. Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization. Proceedings of the National Academy of Sciences of the United States of America. 101: 482-7. PMID 14695884 DOI: 10.1073/pnas.2536901100  1
2004 Tawfik DS. Structure provides insight into sarin enzyme action Industrial Bioprocessing. 26: 9.  1
2003 James LC, Tawfik DS. The specificity of cross-reactivity: promiscuous antibody binding involves specific hydrogen bonds rather than nonspecific hydrophobic stickiness. Protein Science : a Publication of the Protein Society. 12: 2183-93. PMID 14500876 DOI: 10.1110/ps.03172703  1
2003 James LC, Tawfik DS. Conformational diversity and protein evolution--a 60-year-old hypothesis revisited. Trends in Biochemical Sciences. 28: 361-8. PMID 12878003 DOI: 10.1016/S0968-0004(03)00135-X  1
2003 James LC, Roversi P, Tawfik DS. Antibody multispecificity mediated by conformational diversity. Science (New York, N.Y.). 299: 1362-7. PMID 12610298 DOI: 10.1126/science.1079731  1
2003 Griffiths AD, Tawfik DS. Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization. The Embo Journal. 22: 24-35. PMID 12505981 DOI: 10.1093/emboj/cdg014  1
2002 Sepp A, Tawfik DS, Griffiths AD. Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry. Febs Letters. 532: 455-8. PMID 12482612 DOI: 10.1016/S0014-5793(02)03740-7  1
2002 Lee YF, Tawfik DS, Griffiths AD. Investigating the target recognition of DNA cytosine-5 methyltransferase Hhal by library selection using in vitro compartmentalisation Nucleic Acids Research. 30: 4937-4944. PMID 12433997  1
2002 Cohen HM, Tawfik DS, Griffiths AD. Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferase Nucleic Acids Research. 30: 3880-3885. PMID 12202773  1
2002 Lindner AB, Kim SH, Schindler DG, Eshhar Z, Tawfik DS. Esterolytic antibodies as mechanistic and structural models of hydrolases-a quantitative analysis. Journal of Molecular Biology. 320: 559-72. PMID 12096909 DOI: 10.1016/S0022-2836(02)00418-7  1
2001 James LC, Tawfik DS. Catalytic and binding poly-reactivities shared by two unrelated proteins: The potential role of promiscuity in enzyme evolution Protein Science. 10: 2600-2607. PMID 11714928 DOI: 10.1110/ps.ps.14601  1
2001 Hollfelder F, Kirby AJ, Tawfik DS. On the magnitude and specificity of medium effects in enzyme-like catalysts for proton transfer. The Journal of Organic Chemistry. 66: 5866-74. PMID 11511264 DOI: 10.1021/jo015723v  1
2000 Griffiths AD, Tawfik DS. Man-made enzymes - From design to in vitro compartmentalisation Current Opinion in Biotechnology. 11: 338-353. PMID 10975453 DOI: 10.1016/S0958-1669(00)00109-9  1
2000 Kirby AJ, Hollfelder F, Tawfik DS. Nonspecific catalysis by protein surfaces. Applied Biochemistry and Biotechnology. 83: 173-80; discussion 1. PMID 10826958  1
2000 Hollfelder F, Kirby AJ, Tawfik DS, Kikuchi K, Hilvert D. Characterization of proton-transfer catalysis by serum albumins Journal of the American Chemical Society. 122: 1022-1029. DOI: 10.1021/ja993471y  1
1999 Lindner AB, Eshhar Z, Tawfik DS. Conformational changes affect binding and catalysis by ester-hydrolysing antibodies. Journal of Molecular Biology. 285: 421-30. PMID 9878416 DOI: 10.1006/jmbi.1998.2309  1
1998 Tawfik DS, Griffiths AD. Man-made cell-like compartments for molecular evolution Nature Biotechnology. 16: 652-656. PMID 9661199 DOI: 10.1038/nbt0798-652  1
1997 Kim SH, Schindler DG, Lindner AB, Tawfik DS, Eshhar Z. Expression and characterization of recombinant single-chain Fv and Fv fragments derived from a set of catalytic antibodies. Molecular Immunology. 34: 891-906. PMID 9464525 DOI: 10.1016/S0161-5890(97)00096-5  1
1997 Tawfik DS, Lindner AB, Chap R, Eshhar Z, Green BS. Efficient and selective p-nitrophenyl-ester-hydrolyzing antibodies elicited by a p-nitrobenzyl phosphonate hapten. European Journal of Biochemistry / Febs. 244: 619-26. PMID 9119032  1
1997 Charbonnier JB, Golinelli-Pimpaneau B, Gigant B, Tawfik DS, Chap R, Schindler DG, Kim SH, Green BS, Eshhar Z, Knossow M. Structural convergence in the active sites of a family of catalytic antibodies. Science (New York, N.Y.). 275: 1140-2. PMID 9027317 DOI: 10.1126/science.275.5303.1140  1
1997 Hollfelder F, Kirby AJ, Tawfik DS. Efficient catalysis of proton transfer by synzymes Journal of the American Chemical Society. 119: 9578-9579. DOI: 10.1021/ja971970y  1
1996 Hollfelder F, Kirby AJ, Tawfik DS. Off-the-shelf proteins that rival tailor-made antibodies as catalysts. Nature. 383: 60-2. PMID 8779715 DOI: 10.1038/383060a0  1
1995 Tawfik DS, Chap R, Green BS, Sela M, Eshhar Z. Unexpectedly high occurrence of catalytic antibodies in MRL/lpr and SJL mice immunized with a transition-state analog: Is there a linkage to autoimmunity? Proceedings of the National Academy of Sciences of the United States of America. 92: 2145-2149. PMID 7892238 DOI: 10.1073/pnas.92.6.2145  1
1995 Tawfik D, Rapley R. Perspectives in protein engineering and complementary technologies, Oxford, UK, September 13-17, 1994 - To Design or Select: That Is the Question Molecular Biotechnology. 3: 172-173. PMID 7620978 DOI: 10.1007/BF02789114  1
1994 Zemel R, Schindler DG, Tawfik DS, Eshhar Z, Green BS. Differences in the biochemical properties of esterolytic antibodies correlate with structural diversity. Molecular Immunology. 31: 127-37. PMID 8309476 DOI: 10.1016/0161-5890(94)90085-X  1
1994 Tawfik DS, Chap R, Eshhar Z, Green BS. Ph on-off switching of antibody-hapten binding by site-specificchemical modification of tyrosine Protein Engineering, Design and Selection. 7: 431-434. PMID 8177892 DOI: 10.1093/protein/7.3.431  1
1994 Golinelli-Pimpaneau B, Gigant B, Bizebard T, Navaza J, Saludjian P, Zemel R, Tawfik D, Eshhar Z, Green B, Knossow M. Crystal structure of a catalytic antibody Fab with esterase- like activity Structure. 2: 175-183. PMID 8069632 DOI: 10.1016/S0969-2126(00)00019-8  1
1994 Tawfik DS, Eshhar Z, Green BS. Catalytic antibodies: A critical assessment Molecular Biotechnology. 1: 87-103. PMID 7859154 DOI: 10.1007/BF02821512  1
1993 Tawfik DS, Green BS, Chap R, Sela M, Eshhar Z. catELISA: A facile general route to catalytic antibodies Proceedings of the National Academy of Sciences of the United States of America. 90: 373-377. PMID 7678455 DOI: 10.1073/pnas.90.2.373  1
1993 Tawfik DS, Eshhar Z, Bentolila A, Green BS. 1,8-Diazabicyclo[5.4.0]undecene mediated transesterification of p-nitrophenyl phosphonates: A novel route to phosphono esters Synthesis. 968-972.  1
1992 Tawfik DS, Green BS, Eshhar Z. Detection of catalytic monoclonal antibodies Analytical Biochemistry. 202: 35-39. PMID 1621985 DOI: 10.1016/0003-2697(92)90201-H  1
1990 Tawfik DS, Zemel RR, Arad-Yellin R, Green BS, Eshhar Z. Simple method for selecting catalytic monoclonal antibodies that exhibit turnover and specificity Biochemistry. 29: 9916-9921. PMID 2271630  1
1989 Green BS, Tawfik DS. Catalytic monoclonal antibodies: tailor-made, enzyme-like catalysts for chemical reactions Trends in Biotechnology. 7: 304-310. DOI: 10.1016/0167-7799(89)90112-1  1
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