David L. Hacker - Publications

Affiliations: 
The University of Tennessee 
Area:
Microbiology Biology, Molecular Biology

72 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2017 Shen X, Dojcinovic D, Baldi L, Hacker DL, Luescher IF, Wurm FM. Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line. Biotechnology Letters. PMID 28993910 DOI: 10.1007/s10529-017-2440-0  0.56
2016 Zhu LK, Monteil DT, Shen X, Hacker DL, Song BY, Wang ZL, De Jesus M, Wurm FM. Studies on Fluid Dynamics of the Flow Field and Gas Transfer in Orbitally Shaken Tubes. Biotechnology Progress. PMID 27690196 DOI: 10.1002/btpr.2375  0.56
2016 Monteil DT, Juvet V, Paz J, Moniatte M, Baldi L, Hacker DL, Wurm FM. A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation. Biotechnology Progress. PMID 27453130 DOI: 10.1002/btpr.2328  0.56
2016 Hacker DL, Balasubramanian S. Recombinant protein production from stable mammalian cell lines and pools. Current Opinion in Structural Biology. 38: 129-136. PMID 27322762 DOI: 10.1016/j.sbi.2016.06.005  0.96
2016 Balasubramanian S, Hacker DL, Wurm FM. Multigene expression in stable CHO cell pools generated with the piggyBac transposon system. Biotechnology Progress. PMID 27302570 DOI: 10.1002/btpr.2319  0.96
2016 Monteil DT, Shen X, Tontodonati G, Baldi L, Hacker DL, Wurm FM. Disposable orbitally-shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension. Analytical Biochemistry. PMID 27130502 DOI: 10.1016/j.ab.2016.04.009  0.96
2015 Balasubramanian S, Rajendra Y, Baldi L, Hacker DL, Wurm FM. Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines. Biotechnology and Bioengineering. PMID 26616356 DOI: 10.1002/bit.25888  0.96
2015 Shen X, Pitol AK, Bachmann V, Hacker DL, Baldi L, Wurm FM. A simple plasmid-based transient gene expression method using High Five cells. Journal of Biotechnology. 216: 67-75. PMID 26476358 DOI: 10.1016/j.jbiotec.2015.10.007  0.96
2015 Rajendra Y, Balasubramanian S, Kiseljak D, Baldi L, Wurm FM, Hacker DL. Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents. Biotechnology Progress. PMID 26260195 DOI: 10.1002/btpr.2152  0.96
2015 López AR, Dimitrov M, Gerber H, Braman V, Hacker DL, Wurm FM, Fraering PC. Production of active glycosylation-deficient γ-secretase complex for crystallization studies. Biotechnology and Bioengineering. PMID 26059427 DOI: 10.1002/bit.25675  0.96
2015 Balasubramanian S, Matasci M, Kadlecova Z, Baldi L, Hacker DL, Wurm FM. Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools. Journal of Biotechnology. 200: 61-9. PMID 25758242 DOI: 10.1016/j.jbiotec.2015.03.001  0.96
2015 Rajendra Y, Kiseljak D, Baldi L, Wurm FM, Hacker DL. Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells. Biotechnology Progress. 31: 541-9. PMID 25683738 DOI: 10.1002/btpr.2064  0.96
2014 Shen X, Hacker DL, Baldi L, Wurm FM. Virus-free transient protein production in Sf9 cells. Journal of Biotechnology. 171: 61-70. PMID 24333123 DOI: 10.1016/j.jbiotec.2013.11.018  0.96
2013 Hacker DL, Kiseljak D, Rajendra Y, Thurnheer S, Baldi L, Wurm FM. Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells. Protein Expression and Purification. 92: 67-76. PMID 24021764 DOI: 10.1016/j.pep.2013.09.001  0.96
2013 Kadlecova Z, Rajendra Y, Matasci M, Baldi L, Hacker DL, Wurm FM, Klok HA. DNA delivery with hyperbranched polylysine: a comparative study with linear and dendritic polylysine. Journal of Controlled Release : Official Journal of the Controlled Release Society. 169: 276-88. PMID 23379996 DOI: 10.1016/j.jconrel.2013.01.019  0.96
2013 Alattia JR, Matasci M, Dimitrov M, Aeschbach L, Balasubramanian S, Hacker DL, Wurm FM, Fraering PC. Highly efficient production of the Alzheimer's γ-secretase integral membrane protease complex by a multi-gene stable integration approach. Biotechnology and Bioengineering. 110: 1995-2005. PMID 23359429 DOI: 10.1002/bit.24851  0.96
2013 Monteil DT, Tontodonati G, Ghimire S, Baldi L, Hacker DL, Bürki CA, Wurm FM. Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension Biochemical Engineering Journal. 76: 6-12. DOI: 10.1016/j.bej.2013.04.008  0.96
2012 Michel PO, Degen C, Hubert M, Baldi L, Hacker DL, Wurm FM. A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells. Analytical Biochemistry. 430: 138-40. PMID 22960013 DOI: 10.1016/j.ab.2012.08.028  0.96
2012 Rajendra Y, Kiseljak D, Manoli S, Baldi L, Hacker DL, Wurm FM. Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells. Biotechnology and Bioengineering. 109: 2271-8. PMID 22422519 DOI: 10.1002/bit.24494  0.96
2012 Kadlecova Z, Nallet S, Hacker DL, Baldi L, Klok HA, Wurm FM. Poly(ethyleneimine)-mediated large-scale transient gene expression: influence of molecular weight, polydispersity and N-propionyl groups. Macromolecular Bioscience. 12: 628-36. PMID 22411776 DOI: 10.1002/mabi.201100404  0.96
2012 Tissot S, Michel PO, Hacker DL, Baldi L, De Jesus M, Wurm FM. k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors. New Biotechnology. 29: 387-94. PMID 22142586 DOI: 10.1016/j.nbt.2011.10.010  0.96
2012 Rajendra Y, Kiseljak D, Baldi L, Hacker DL, Wurm FM. Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells. Biotechnology Letters. 34: 619-26. PMID 22127760 DOI: 10.1007/s10529-011-0809-z  0.96
2012 Baldi L, Hacker DL, Meerschman C, Wurm FM. Large-scale transfection of mammalian cells. Methods in Molecular Biology (Clifton, N.J.). 801: 13-26. PMID 21987244 DOI: 10.1007/978-1-61779-352-3_2  0.96
2011 Matasci M, Bachmann V, Baldi L, Hacker DL, De Jesus M, Wurm FM. Rapid recombinant protein production from pools of transposon-generated CHO cells. Bmc Proceedings. 5: P34. PMID 22373498 DOI: 10.1186/1753-6561-5-S8-P34  0.56
2011 Kiseljak D, Rajendra Y, Manoli SS, Baldi L, Hacker DL, Wurm FM. The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells. Bmc Proceedings. 5: P33. PMID 22373474 DOI: 10.1186/1753-6561-5-S8-P33  0.56
2011 Balasubramanian S, Matasci M, Baldi L, Hacker DL, Wurm FM. Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells. Bmc Proceedings. 5: P32. PMID 22373439 DOI: 10.1186/1753-6561-5-S8-P32  0.56
2011 Matasci M, Bachmann V, Baldi L, Hacker DL, De Jesus M, Wurm FM. CHO cell lines generated by PiggyBac transposition. Bmc Proceedings. 5: P31. PMID 22373414 DOI: 10.1186/1753-6561-5-S8-P31  0.56
2011 Tissot S, Reclari M, Quinodoz S, Dreyer M, Monteil DT, Baldi L, Hacker DL, Farhat M, Discacciati M, Quarteroni A, Wurm FM. Hydrodynamic stress in orbitally shaken bioreactors. Bmc Proceedings. 5: P39. PMID 22373127 DOI: 10.1186/1753-6561-5-S8-P39  0.56
2011 Pereira J, Rajendra Y, Baldi L, Hacker DL, Wurm FM. Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin(®) bioreactors. Bmc Proceedings. 5: P38. PMID 22373096 DOI: 10.1186/1753-6561-5-S8-P38  0.56
2011 Shen X, Michel PO, Xie Q, Hacker DL, Wurm FM. Transient transfection of insect Sf-9 cells in TubeSpin(®) bioreactor 50 tubes. Bmc Proceedings. 5: P37. PMID 22373074 DOI: 10.1186/1753-6561-5-S8-P37  0.56
2011 Tissot S, Monteil DT, Baldi L, Hacker DL, Wurm FM. kLa as a predictor for probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors. Bmc Proceedings. 5: P36. PMID 22373050 DOI: 10.1186/1753-6561-5-S8-P36  0.56
2011 Rajendra Y, Kiseljak D, Baldi L, Hacker DL, Wurm FM. Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells. Bmc Proceedings. 5: P35. PMID 22373018 DOI: 10.1186/1753-6561-5-S8-P35  0.56
2011 Matasci M, Baldi L, Hacker DL, Wurm FM. The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability. Biotechnology and Bioengineering. 108: 2141-50. PMID 21495018 DOI: 10.1002/bit.23167  0.96
2011 Rajendra Y, Kiseljak D, Baldi L, Hacker DL, Wurm FM. A simple high-yielding process for transient gene expression in CHO cells. Journal of Biotechnology. 153: 22-6. PMID 21392548 DOI: 10.1016/j.jbiotec.2011.03.001  0.96
2011 Tissot S, Oberbek A, Reclari M, Dreyer M, Hacker DL, Baldi L, Farhat M, Wurm FM. Efficient and reproducible mammalian cell bioprocesses without probes and controllers? New Biotechnology. 28: 382-90. PMID 21338722 DOI: 10.1016/j.nbt.2011.02.004  0.96
2011 Xie Q, Michel PO, Baldi L, Hacker DL, Zhang X, Wurm FM. TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension. Biotechnology Letters. 33: 897-902. PMID 21267765 DOI: 10.1007/s10529-011-0527-6  0.96
2011 Oberbek A, Matasci M, Hacker DL, Wurm FM. Generation of stable, high-producing CHO cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture. Biotechnology and Bioengineering. 108: 600-10. PMID 20967750 DOI: 10.1002/bit.22968  0.96
2010 Zhang X, Garcia IF, Baldi L, Hacker DL, Wurm FM. Hyperosmolarity enhances transient recombinant protein yield in Chinese hamster ovary cells. Biotechnology Letters. 32: 1587-92. PMID 20574835 DOI: 10.1007/s10529-010-0331-8  0.96
2010 Wulhfard S, Baldi L, Hacker DL, Wurm F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology. 148: 128-32. PMID 20510314 DOI: 10.1016/j.jbiotec.2010.05.003  0.96
2010 Tissot S, Farhat M, Hacker DL, Anderlei T, Kühner M, Comninellis C, Wurm F. Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors Biochemical Engineering Journal. 52: 181-186. DOI: 10.1016/j.bej.2010.08.005  0.96
2009 Nallet S, Amacker M, Westerfeld N, Baldi L, König I, Hacker DL, Zaborosch C, Zurbriggen R, Wurm FM. Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells. Vaccine. 27: 6415-9. PMID 19559121 DOI: 10.1016/j.vaccine.2009.06.019  0.96
2009 Hacker DL, De Jesus M, Wurm FM. 25 years of recombinant proteins from reactor-grown cells - where do we go from here? Biotechnology Advances. 27: 1023-7. PMID 19463938 DOI: 10.1016/j.biotechadv.2009.05.008  0.96
2009 Zhang X, Bürki CA, Stettler M, De Sanctis D, Perrone M, Discacciati M, Parolini N, DeJesus M, Hacker DL, Quarteroni A, Wurm FM. Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1000 L Biochemical Engineering Journal. 45: 41-47. DOI: 10.1016/j.bej.2009.02.003  0.96
2008 Matasci M, Hacker DL, Baldi L, Wurm FM. Recombinant therapeutic protein production in cultivated mammalian cells: current status and future prospects. Drug Discovery Today. Technologies. 5: e37-42. PMID 24981089 DOI: 10.1016/j.ddtec.2008.12.003  0.96
2008 Chenuet S, Martinet D, Besuchet-Schmutz N, Wicht M, Jaccard N, Bon AC, Derouazi M, Hacker DL, Beckmann JS, Wurm FM. Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection. Biotechnology and Bioengineering. 101: 937-45. PMID 18781700 DOI: 10.1002/bit.21972  0.96
2008 Bertschinger M, Schertenleib A, Cevey J, Hacker DL, Wurm FM. The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells. Molecular Biotechnology. 40: 136-43. PMID 18543131 DOI: 10.1007/s12033-008-9069-0  0.96
2008 Zhang X, Stettler M, Reif O, Kocourek A, Dejesus M, Hacker DL, Wurm FM. Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000 L by surface aeration with air. New Biotechnology. 25: 68-75. PMID 18504021 DOI: 10.1016/j.nbt.2008.03.001  0.96
2008 Backliwal G, Hildinger M, Kuettel I, Delegrange F, Hacker DL, Wurm FM. Valproic acid: a viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures. Biotechnology and Bioengineering. 101: 182-9. PMID 18454496 DOI: 10.1002/bit.21882  0.96
2008 Wulhfard S, Tissot S, Bouchet S, Cevey J, De Jesus M, Hacker DL, Wurm FM. Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells. Biotechnology Progress. 24: 458-65. PMID 18220408 DOI: 10.1021/bp070286c  0.96
2007 Stettler M, Zhang X, Hacker DL, De Jesus M, Wurm FM. Novel orbital shake bioreactors for transient production of CHO derived IgGs. Biotechnology Progress. 23: 1340-6. PMID 17914862 DOI: 10.1021/bp070219i  0.96
2007 Muller N, Derouazi M, Van Tilborgh F, Wulhfard S, Hacker DL, Jordan M, Wurm FM. Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems. Biotechnology Letters. 29: 703-11. PMID 17310326 DOI: 10.1007/s10529-006-9298-x  0.96
2007 Baldi L, Hacker DL, Adam M, Wurm FM. Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives. Biotechnology Letters. 29: 677-84. PMID 17235486 DOI: 10.1007/s10529-006-9297-y  0.96
2006 Bertschinger M, Backliwal G, Schertenleib A, Jordan M, Hacker DL, Wurm FM. Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery. Journal of Controlled Release : Official Journal of the Controlled Release Society. 116: 96-104. PMID 17079047 DOI: 10.1016/j.jconrel.2006.09.006  0.96
2006 Grosjean F, Bertschinger M, Hacker DL, Wurm FM. Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells. Biotechnology Letters. 28: 1827-33. PMID 17009090 DOI: 10.1007/s10529-006-9167-7  0.96
2006 Bertschinger M, Burki C, Backliwal G, Hacker DL, Jordan M, Wurm FM. Polyethylenimine-based quality control assay for plasmid DNA. Analytical Biochemistry. 356: 309-11. PMID 16884675 DOI: 10.1016/j.ab.2006.05.002  0.96
2006 Derouazi M, Martinet D, Besuchet Schmutz N, Flaction R, Wicht M, Bertschinger M, Hacker DL, Beckmann JS, Wurm FM. Genetic characterization of CHO production host DG44 and derivative recombinant cell lines Biochemical and Biophysical Research Communications. 340: 1069-1077. PMID 16403443 DOI: 10.1016/j.bbrc.2005.12.111  0.96
2005 Hacker DL, Derow E, Wurm FM. The CELO adenovirus Gam1 protein enhances transient and stable recombinant protein expression in Chinese hamster ovary cells. Journal of Biotechnology. 117: 21-9. PMID 15831244 DOI: 10.1016/j.jbiotec.2005.01.006  0.96
2005 Muller N, Girard P, Hacker DL, Jordan M, Wurm FM. Orbital shaker technology for the cultivation of mammalian cells in suspension. Biotechnology and Bioengineering. 89: 400-6. PMID 15619325 DOI: 10.1002/bit.20358  0.96
2005 Hunt L, Hacker DL, Grosjean F, De Jesus M, Uebersax L, Jordan M, Wurm FM. Low-temperature pausing of cultivated mammalian cells. Biotechnology and Bioengineering. 89: 157-63. PMID 15584025 DOI: 10.1002/bit.20320  0.96
2004 Hacker DL, Bertschinger M, Baldi L, Wurm FM. Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells. Gene. 341: 227-34. PMID 15474305 DOI: 10.1016/j.gene.2004.06.054  0.96
2001 Lee SK, Dabney-Smith C, Hacker DL, Bruce BD. Membrane activity of the southern cowpea mosaic virus coat protein: the role of basic amino acids, helix-forming potential, and lipid composition. Virology. 291: 299-310. PMID 11878899 DOI: 10.1006/viro.2001.1210  0.96
2001 Lee SK, Hacker DL. In vitro analysis of an RNA binding site within the N-terminal 30 amino acids of the southern cowpea mosaic virus coat protein. Virology. 286: 317-27. PMID 11485399 DOI: 10.1006/viro.2001.0979  0.96
2000 Hacker DL, Fowler BC. Complementation of the host range restriction of southern cowpea mosaic virus in bean by southern bean mosaic virus. Virology. 266: 140-9. PMID 10612668 DOI: 10.1006/viro.1999.0072  0.96
1998 Sivakumaran K, Fowler BC, Hacker DL. Identification of viral genes required for cell-to-cell movement of southern bean mosaic virus. Virology. 252: 376-86. PMID 9878617 DOI: 10.1006/viro.1998.9489  0.96
1998 Sivakumaran K, Hacker DL. The 105-kDa polyprotein of southern bean mosaic virus is translated by scanning ribosomes. Virology. 246: 34-44. PMID 9656991 DOI: 10.1006/viro.1998.9183  0.96
1997 Hacker DL, Sivakumaran K. Mapping and expression of southern bean mosaic virus genomic and subgenomic RNAs. Virology. 234: 317-27. PMID 9268164 DOI: 10.1006/viro.1997.8667  0.96
1995 Bowen MD, Jackson AO, Bruns TD, Hacker DL, Hardy JL. Determination and comparative analysis of the small RNA genomic sequences of California encephalitis, Jamestown Canyon, Jerry Slough, Melao, Keystone and Trivittatus viruses (Bunyaviridae, genus Bunyavirus, California serogroup). The Journal of General Virology. 76: 559-72. PMID 7897347  0.96
1995 Hacker DL. Identification of a coat protein binding site on southern bean mosaic virus RNA. Virology. 207: 562-5. PMID 7886960 DOI: 10.1006/viro.1995.1117  0.96
1992 Hacker DL, Petty IT, Wei N, Morris TJ. Turnip crinkle virus genes required for RNA replication and virus movement. Virology. 186: 1-8. PMID 1727594 DOI: 10.1016/0042-6822(92)90055-T  0.96
1992 Wei N, Hacker DL, Morris TJ. Characterization of an internal element in turnip crinkle virus RNA involved in both coat protein binding and replication. Virology. 190: 346-55. PMID 1529538 DOI: 10.1016/0042-6822(92)91221-F  0.96
1989 Hacker DL, Fluck MM. Viral DNA synthesis in nonpermissive rat F-111 cells and its role in neoplastic transformation by polyomavirus Molecular and Cellular Biology. 9: 648-658. PMID 2540424  0.96
1989 Hacker DL, Friderici K, Fluck MM. A nonlethal mutation in large T antigen of polyomavirus which affects viral DNA synthesis Journal of Virology. 63: 776-781. PMID 2536103  0.96
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