| Year |
Citation |
Score |
| 2023 |
Calvert M, Sweeney RP, Chen HM, Bajwa H, Nasseri SA, Habibi D, Withers SG. Branched montbretin A mimics allow derivatisation and potent amylase inhibition. Organic & Biomolecular Chemistry. 21: 7977-7983. PMID 37751159 DOI: 10.1039/d3ob01051a |
0.78 |
|
| 2023 |
Prasch H, Wolfsgruber A, Thonhofer M, Culum A, Mandl C, Weber P, Zündel M, Nasseri SA, Gonzalez Santana A, Tegl G, Nidetzky B, Gruber K, Stütz AE, Withers SG, Wrodnigg TM. Ligand-Directed Chemistry on Glycoside Hydrolases - A Proof of Concept Study. Chembiochem : a European Journal of Chemical Biology. 24: e202300480. PMID 37715738 DOI: 10.1002/cbic.202300480 |
0.305 |
|
| 2023 |
Danby PM, Jeong A, Sim L, Sweeney RP, Wardman JF, Karimi R, Geissner A, Worrall LJ, Reid JP, Strynadka NCJ, Withers S. Vinyl Halide-Modified Unsaturated Cyclitols are Mechanism-Based Glycosidase Inhibitors. Angewandte Chemie (International Ed. in English). e202301258. PMID 36940280 DOI: 10.1002/anie.202301258 |
0.325 |
|
| 2022 |
Hunter C, Gao Z, Chen HM, Thompson N, Wakarchuk W, Nitz M, Withers SG, Willis LM. Attenuation of Polysialic Acid Biosynthesis in Cells by the Small Molecule Inhibitor 8-Keto-sialic acid. Acs Chemical Biology. 18: 41-48. PMID 36577399 DOI: 10.1021/acschembio.2c00638 |
0.695 |
|
| 2022 |
Santana A, Robinson K, Vickers C, Deen M, Chen HM, Zhou S, Dai B, Fuller M, Boraston A, Vocadlo D, Clarke L, Withers S. Pharmacological Chaperones for GCase That Switch Conformation with pH Enhance Enzyme Levels in Gaucher Animal Models. Angewandte Chemie (International Ed. in English). PMID 35864061 DOI: 10.1002/anie.202207974 |
0.551 |
|
| 2021 |
Tegl G, Rahfeld P, Ostmann K, Hanson J, Withers SG. Discovery of β--acetylglucosaminidases from screening metagenomic libraries and their use as thioglycoligase mutants. Organic & Biomolecular Chemistry. PMID 34622263 DOI: 10.1039/d1ob01246k |
0.302 |
|
| 2021 |
Gao J, Klenow L, Parsons L, Malik T, Phue JN, Gao Z, Withers SG, Cipollo J, Daniels R, Wan H. Design of the Recombinant Influenza Neuraminidase Antigen is Crucial for its Biochemical Properties and Protective Efficacy. Journal of Virology. JVI0116021. PMID 34613807 DOI: 10.1128/JVI.01160-21 |
0.665 |
|
| 2021 |
Li J, Solhi L, Goddard-Borger ED, Mathieu Y, Wakarchuk WW, Withers SG, Brumer H. Four cellulose-active lytic polysaccharide monooxygenases from Cellulomonas species. Biotechnology For Biofuels. 14: 29. PMID 33485381 DOI: 10.1186/s13068-020-01860-3 |
0.613 |
|
| 2020 |
Harmsen RAG, Aam BB, Madhuprakash J, Hamre AG, Goddard-Borger ED, Withers SG, Eijsink VGH, Sørlie M. Chemoenzymatic Synthesis of Chito-oligosaccharides with Alternating -d-Acetylglucosamine and d-Glucosamine. Biochemistry. 59: 4581-4590. PMID 33213137 DOI: 10.1021/acs.biochem.0c00839 |
0.641 |
|
| 2020 |
Fonseca MJMD, Armstrong Z, Withers SG, Briers Y. High-throughput generation of product profiles for arabinoxylan-active enzymes from metagenomes. Applied and Environmental Microbiology. PMID 32948521 DOI: 10.1128/Aem.01505-20 |
0.418 |
|
| 2020 |
Weber P, Thonhofer M, Averill S, Davies GJ, Santana AG, Müller P, Nasseri SA, Offen WA, Pabst BM, Paschke E, Schalli M, Torvisco A, Tschernutter M, Tysoe C, Windischhofer W, ... Withers SG, et al. Mechanistic Insights into the Chaperoning of Human Lysosomal-Galactosidase Activity: Highly Functionalized Aminocyclopentanes and -5a-Substituted Derivatives of 4--Isofagomine. Molecules (Basel, Switzerland). 25. PMID 32899288 DOI: 10.3390/Molecules25174025 |
0.36 |
|
| 2020 |
Zhao J, Si Y, Han Z, Li J, Guo W, Fu Y, Tegl G, Hanson J, Chen HM, Kwan DH, Santana AG, Withers SG. Corrigendum: Facile Formation of β-thioGlcNAc Linkages to Thiol-Containing Sugars, Peptides, and Proteins using a Mutant GH20 Hexosaminidase. Angewandte Chemie (International Ed. in English). 59: 8752. PMID 32449594 DOI: 10.1002/anie.202001582 |
0.658 |
|
| 2019 |
Tysoe CR, Caner S, Calvert MB, Win-Mason A, Brayer GD, Withers SG. Synthesis of montbretin A analogues yields potent competitive inhibitors of human pancreatic α-amylase. Chemical Science. 10: 11073-11077. PMID 32206255 DOI: 10.1039/c9sc02610j |
0.76 |
|
| 2019 |
Gao Z, Robinson K, Skowronski DM, De Serres G, Withers SG. Quantification of the total neuraminidase content of recent commercially-available influenza vaccines: Introducing a neuraminidase titration reagent. Vaccine. PMID 31818533 DOI: 10.1016/J.Vaccine.2019.11.041 |
0.712 |
|
| 2019 |
Tan Y, Zhang Y, Han Y, Liu H, Chen H, Ma F, Withers SG, Feng Y, Yang G. Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method. Science Advances. 5: eaaw8451. PMID 31633018 DOI: 10.1126/Sciadv.Aaw8451 |
0.374 |
|
| 2019 |
Rahfeld P, Wardman JF, Mehr K, Huff D, Morgan-Lang C, Chen HM, Hallam SJ, Withers SG. Prospecting for α-N-acetylgalactosaminidases yields a new class of GH31 O-glycanase. The Journal of Biological Chemistry. PMID 31530641 DOI: 10.1074/Jbc.Ra119.010628 |
0.361 |
|
| 2019 |
McKimm-Breschkin JL, Barrett S, McKenzie-Kludas C, McAuley J, Streltsov VA, Withers SG. Passaging of an influenza A(H1N1)pdm09 virus in a difluoro sialic acid inhibitor selects for a novel, but unfit I106M neuraminidase mutant. Antiviral Research. 104542. PMID 31233807 DOI: 10.1016/J.Antiviral.2019.104542 |
0.308 |
|
| 2019 |
Rahfeld P, Sim L, Moon H, Constantinescu I, Morgan-Lang C, Hallam SJ, Kizhakkedathu JN, Withers SG. An enzymatic pathway in the human gut microbiome that converts A to universal O type blood. Nature Microbiology. PMID 31182795 DOI: 10.1038/S41564-019-0469-7 |
0.345 |
|
| 2019 |
Zoidl M, Wolfsgruber A, Schalli M, Nasseri SA, Weber P, Stütz AE, Withers SG, Wrodnigg TM. Synthesis of modified 1,5-imino-d-xylitols as ligands for lysosomal -glucocerebrosidase. Monatshefte Fur Chemie. 150: 831-842. PMID 31178604 DOI: 10.1007/S00706-019-02427-1 |
0.312 |
|
| 2019 |
Macdonald SS, Armstrong Z, Morgan-Lang C, Osowiecka M, Robinson K, Hallam SJ, Withers SG. Development and Application of a High-Throughput Functional Metagenomic Screen for Glycoside Phosphorylases. Cell Chemical Biology. PMID 31080075 DOI: 10.1016/J.Chembiol.2019.03.017 |
0.338 |
|
| 2019 |
Strazzulli A, Perugino G, Mazzone M, Rossi M, Withers SG, Moracci M. Probing the role of an invariant active site His in family GH1 β-glycosidases. Journal of Enzyme Inhibition and Medicinal Chemistry. 34: 973-980. PMID 31072150 DOI: 10.1080/14756366.2019.1608198 |
0.442 |
|
| 2019 |
Janesch B, Baumann L, Mark A, Thompson N, Rahmani S, Sim L, Withers SG, Wakarchuk WW. Directed evolution of bacterial polysialyltransferases. Glycobiology. PMID 30976781 DOI: 10.1093/Glycob/Cwz021 |
0.307 |
|
| 2019 |
Gao Z, Ovchinnikova OG, Huang BS, Liu F, Williams DE, Andersen R, Lowary TL, Whitfield C, Withers SG. A High-Throughput "FP-Tag" Assay for the Identification of Glycosyltransferase Inhibitors. Journal of the American Chemical Society. PMID 30698425 DOI: 10.1021/Jacs.8B10940 |
0.741 |
|
| 2019 |
Tysoe CR, Caner S, Calvert MB, Win-Mason A, Brayer GD, Withers SG. Synthesis of montbretin A analogues yields potent competitive inhibitors of human pancreatic alpha amylase Chemical Science. 10: 11073-11077. DOI: 10.2210/Pdb6Ocn/Pdb |
0.361 |
|
| 2019 |
Armstrong Z, Liu F, Chen H, Hallam SJ, Withers SG. Systematic Screening of Synthetic Gene-Encoded Enzymes for Synthesis of Modified Glycosides Acs Catalysis. 9: 3219-3227. DOI: 10.1021/Acscatal.8B05179 |
0.415 |
|
| 2019 |
Schalli M, Weber P, Nasseri SA, Gomez AT, Müller P, Stütz A, Withers SG, Wolfsgruber A, Wrodnigg TM. Biologically active branched-chain aminocyclopentane tetraols from D-galactose Monatshefte Fur Chemie. 150: 861-870. DOI: 10.1007/S00706-019-02428-0 |
0.363 |
|
| 2018 |
Tegl G, Hanson J, Chen HM, Kwan DH, Santana AG, Withers SG. Facile Formation of β-thioGlcNAc Linkages to Thiol-Containing Sugars, Peptides, and Proteins using a Mutant GH20 Hexosaminidase. Angewandte Chemie (International Ed. in English). PMID 30549167 DOI: 10.1002/Anie.201809928 |
0.733 |
|
| 2018 |
Brockerman JA, Okon M, Withers SG, McIntosh LP. The pK values of the catalytic residues in the retaining glycoside hydrolase T26H mutant of T4 lysozyme. Protein Science : a Publication of the Protein Society. PMID 30537432 DOI: 10.1002/Pro.3562 |
0.396 |
|
| 2018 |
Vickers C, Liu F, Abe K, Salama-Alber O, Jenkins ML, Springate CM, Burke JE, Withers SG, Boraston AB. Endo-fucoidan hydrolases from glycoside hydrolase family 107 (GH107) display structural and mechanistic similarities to α-L-fucosidases from GH29. The Journal of Biological Chemistry. PMID 30282808 DOI: 10.2210/Pdb6Dns/Pdb |
0.413 |
|
| 2018 |
Gao Z, Thompson AJ, Paulson JC, Withers SG. Proximity Ligation-Based Fluorogenic Imaging Agents for Neuraminidases. Angewandte Chemie (International Ed. in English). PMID 30216626 DOI: 10.1002/Anie.201808196 |
0.694 |
|
| 2018 |
Perrella NN, Withers SG, Lopes AR. Identity and role of the non-Conserved Acid/Base Catalytic residue in the GH29 fucosidase from the spider Nephilingis cruentata. Glycobiology. PMID 30204861 DOI: 10.1093/Glycob/Cwy083 |
0.394 |
|
| 2018 |
Tysoe C, Withers SG. Structural Dissection of Helianthamide Reveals the Basis of Its Potent Inhibition of Human Pancreatic α-Amylase. Biochemistry. 57: 5384-5387. PMID 30180544 DOI: 10.1021/Acs.Biochem.8B00825 |
0.408 |
|
| 2018 |
Blaukopf M, Worrall L, Kosma P, Strynadka NCJ, Withers SG. Insights into Heptosyltransferase I Catalysis and Inhibition through the Structure of Its Ternary Complex. Structure (London, England : 1993). PMID 30122450 DOI: 10.1016/J.Str.2018.07.001 |
0.346 |
|
| 2018 |
Chen HM, Withers SG. Synthesis of azido-deoxy and amino-deoxy glycosides and glycosyl fluorides for screening of glycosidase libraries and assembly of substituted glycosides. Carbohydrate Research. 467: 33-44. PMID 30075363 DOI: 10.1016/J.Carres.2018.07.007 |
0.369 |
|
| 2018 |
Armstrong Z, Mewis K, Liu F, Morgan-Lang C, Scofield M, Durno E, Chen HM, Mehr K, Withers SG, Hallam SJ. Metagenomics reveals functional synergy and novel polysaccharide utilization loci in the Castor canadensis fecal microbiome. The Isme Journal. PMID 30013164 DOI: 10.1038/S41396-018-0215-9 |
0.316 |
|
| 2018 |
Nasseri SA, Betschart L, Opaleva D, Rahfeld P, Withers SG. A Mechanism-Based Approach to Screening Metagenomic Libraries for Discovery of Unconventional Glycosidases. Angewandte Chemie (International Ed. in English). PMID 30001477 DOI: 10.1002/Anie.201806792 |
0.386 |
|
| 2018 |
Irmisch S, Jo S, Roach CR, Jancsik S, Yuen MMS, Madilao L, Mark O'Neil-Johnson M, Williams R, Withers SG, Bohlmann J. Discovery of UDP-Glycosyltransferases and BAHD-Acyltransferases in the Biosynthesis of the Anti-Diabetic Plant Metabolite Montbretin A. The Plant Cell. PMID 29967287 DOI: 10.1105/Tpc.18.00406 |
0.343 |
|
| 2018 |
Koetzler MP, Robinson K, Chen HM, Okon M, McIntosh LP, Withers SG. Modulating the nucleophile of a glycoside hydrolase through site-specific incorporation of fluoroglutamic acids. Journal of the American Chemical Society. PMID 29894173 DOI: 10.1021/Jacs.8B04235 |
0.447 |
|
| 2018 |
Hettle AG, Vickers C, Robb CS, Liu F, Withers SG, Hehemann JH, Boraston AB. The Molecular Basis of Polysaccharide Sulfatase Activity and a Nomenclature for Catalytic Subsites in this Class of Enzyme. Structure (London, England : 1993). PMID 29681469 DOI: 10.1016/J.Str.2018.03.012 |
0.395 |
|
| 2018 |
Smirnova I, Kasho V, Jiang X, Chen HM, Withers SG, Kaback HR. Oversized galactosides as a probe for conformational dynamics in LacY. Proceedings of the National Academy of Sciences of the United States of America. PMID 29602806 DOI: 10.1073/Pnas.1800706115 |
0.341 |
|
| 2018 |
Weber P, Nasseri SA, Pabst BM, Torvisco A, Müller P, Paschke E, Tschernutter M, Windischhofer W, Withers SG, Wrodnigg TM, Stütz AE. Potent GH20 N-Acetyl-β-d-hexosaminidase Inhibitors: N-Substituted 3-acetamido-4-amino-5-hydroxymethyl-cyclopentanediols. Molecules (Basel, Switzerland). 23. PMID 29558439 DOI: 10.3390/Molecules23030708 |
0.342 |
|
| 2018 |
Macdonald SS, Patel A, Larmour VLC, Morgan-Lang C, Hallam SJ, Mark BL, Withers SG. Structural and mechanistic analysis of a β-glycoside phosphorylase identified by screening a metagenomic library. The Journal of Biological Chemistry. PMID 29317495 DOI: 10.1074/Jbc.Ra117.000948 |
0.4 |
|
| 2018 |
Abbott W, Alber O, Bayer E, Berrin J, Boraston A, Brumer H, Brzezinski R, Clarke A, Cobucci-Ponzano B, Cockburn D, Coutinho P, Czjzek M, Dassa B, Davies GJ, Eijsink V, ... ... Withers S, et al. Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes Glycobiology. 28: 3-8. PMID 29040563 DOI: 10.1093/Glycob/Cwx089 |
0.806 |
|
| 2017 |
Duo T, Robinson K, Greig IR, Chen HM, Patrick BO, Withers SG. REMARKABLE REACTIVITY DIFFERENCES BETWEEN GLUCOSIDES WITH IDENTICAL LEAVING GROUPS. Journal of the American Chemical Society. PMID 29035043 DOI: 10.1021/Jacs.7B09645 |
0.38 |
|
| 2017 |
Saxena H, Hsu B, de Asis M, Zierke M, Sim L, Withers SG, Wakarchuk W. Characterization of a Thermostable Endoglucanase from Cellulomonas fimi ATCC484. Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. PMID 28982013 DOI: 10.1139/Bcb-2017-0150 |
0.386 |
|
| 2017 |
Armstrong Z, Rahfeld P, Withers SG. Discovery of New Glycosidases From Metagenomic Libraries. Methods in Enzymology. 597: 3-23. PMID 28935108 DOI: 10.1016/Bs.Mie.2017.06.001 |
0.306 |
|
| 2017 |
Mageroy MH, Jancsik S, Yuen MMS, Fischer M, Withers SG, Paetz C, Schneider B, MacKay JJ, Bohlmann J. A conifer UDP-sugar dependent glycosyltransferase contributes to acetophenone metabolism and defense against insects. Plant Physiology. PMID 28794260 DOI: 10.1104/Pp.17.00611 |
0.336 |
|
| 2017 |
Li Z, Fischer M, Satkunarajah M, Zhou D, Withers SG, Rini JM. Structural basis of Notch O-glucosylation and O-xylosylation by mammalian protein-O-glucosyltransferase 1 (POGLUT1). Nature Communications. 8: 185. PMID 28775322 DOI: 10.1038/S41467-017-00255-7 |
0.312 |
|
| 2017 |
Danby PM, Withers SG. Glycosyl Cations versus Allylic Cations in Spontaneous and Enzymatic Hydrolysis. Journal of the American Chemical Society. PMID 28737389 DOI: 10.1021/Jacs.7B05628 |
0.441 |
|
| 2017 |
Lizak C, Worrall LJ, Baumann L, Pfleiderer MM, Volkers G, Sun T, Sim L, Wakarchuk W, Withers SG, Strynadka NCJ. X-ray crystallographic structure of a bacterial polysialyltransferase provides insight into the biosynthesis of capsular polysialic acid. Scientific Reports. 7: 5842. PMID 28724897 DOI: 10.1038/S41598-017-05627-Z |
0.393 |
|
| 2017 |
Schalli M, Weber P, Tysoe C, Pabst BM, Thonhofer M, Paschke E, Stütz AE, Tschernutter M, Windischhofer W, Withers SG. A new type of pharmacological chaperone for GM1-gangliosidosis related human lysosomal β-galactosidase: N-Substituted 5-amino-1-hydroxymethyl-cyclopentanetriols. Bioorganic & Medicinal Chemistry Letters. PMID 28600215 DOI: 10.1016/J.Bmcl.2017.05.086 |
0.336 |
|
| 2017 |
Kötzler MP, McIntosh LP, Withers SG. Refolding the unfoldable: A systematic approach for renaturation of Bacillus circulans xylanase. Protein Science : a Publication of the Protein Society. PMID 28466501 DOI: 10.1002/Pro.3181 |
0.316 |
|
| 2017 |
Schalli M, Tysoe C, Fischer R, Pabst BM, Thonhofer M, Paschke E, Rappitsch T, Stütz AE, Tschernutter M, Windischhofer W, Withers SG. N-Substituted 5-amino-1-hydroxymethyl-cyclopentanetriols: A new family of activity promotors for a GM1-gangliosidosis related human lysosomal β-galactosidase mutant. Carbohydrate Research. 443: 15-22. PMID 28319682 DOI: 10.1016/J.Carres.2017.03.009 |
0.371 |
|
| 2017 |
Lebl R, Thonhofer M, Tysoe C, Pabst BM, Schalli M, Weber P, Paschke E, Stütz AE, Tschernutter M, Windischhofer W, Withers SG. A Morita-Baylis-Hillman based route to C-5a-chain-extended 4-epi-isofagomine type glycosidase inhibitors. Carbohydrate Research. 442: 31-40. PMID 28288345 DOI: 10.1016/J.Carres.2017.03.003 |
0.309 |
|
| 2017 |
Jongkees SA, Caner S, Tysoe C, Brayer GD, Withers SG, Suga H. Rapid Discovery of Potent and Selective Glycosidase-Inhibiting De Novo Peptides. Cell Chemical Biology. PMID 28262556 DOI: 10.1016/J.Chembiol.2017.02.001 |
0.344 |
|
| 2017 |
Kuatsjah E, Chen HM, Withers SG, Eltis LD. Characterization of an extradiol dioxygenase involved in the catabolism of lignin-derived biphenyl. Febs Letters. PMID 28247503 DOI: 10.1002/1873-3468.12611 |
0.317 |
|
| 2017 |
Gao Z, Niikura M, Withers SG. Ultrasensitive Fluorogenic Reagents for Neuraminidase Titration. Angewandte Chemie (International Ed. in English). PMID 28191709 DOI: 10.1002/Anie.201610544 |
0.693 |
|
| 2017 |
Strazzulli A, Cobucci-Ponzano B, Carillo S, Bedini E, Corsaro MM, Pocsfalvi G, Withers SG, Rossi M, Moracci M. Introducing transgalactosylation activity into a family 42 β-galactosidase. Glycobiology. PMID 28158578 DOI: 10.1093/Glycob/Cwx013 |
0.413 |
|
| 2017 |
Schalli M, Wolfsgruber A, Gonzalez Santana A, Tysoe C, Fischer R, Stütz AE, Thonhofer M, Withers SG. C-5a-substituted validamine type glycosidase inhibitors. Carbohydrate Research. 440: 1-9. PMID 28135569 DOI: 10.1016/J.Carres.2017.01.006 |
0.312 |
|
| 2017 |
Centko RM, Ratnaweera PB, Tysoe C, Withers SG, de Silva ED, Andersen RJ. Alpha-glucosidase and alpha-amylase inhibiting thiodiketopiperazines from the endophytic fungus Setosphaeria rostrata isolated from the medicinal plant Costus speciosus in Sri Lanka Phytochemistry Letters. 22: 76-80. DOI: 10.1016/J.Phytol.2017.09.004 |
0.31 |
|
| 2016 |
Sobhanifar S, Worrall LJ, King DT, Wasney GA, Baumann L, Gale RT, Nosella M, Brown ED, Withers SG, Strynadka NC. Structure and Mechanism of Staphylococcus aureus TarS, the Wall Teichoic Acid β-glycosyltransferase Involved in Methicillin Resistance. Plos Pathogens. 12: e1006067. PMID 27973583 DOI: 10.1371/Journal.Ppat.1006067 |
0.388 |
|
| 2016 |
Santana AG, Tysoe C, Hu G, Kronstad J, Goddard-Borger E, Withers S. Fungal glycolipid hydrolase inhibitors and their effect on Cryptococcus neoformans. Chembiochem : a European Journal of Chemical Biology. PMID 27905163 DOI: 10.1002/Cbic.201600538 |
0.654 |
|
| 2016 |
Zhang X, Caner S, Kwan E, Li C, Brayer GD, Withers SG. Evaluation of the significance of starch surface binding sites on human pancreatic α-amylase. Biochemistry. PMID 27756128 DOI: 10.1021/Acs.Biochem.6B00992 |
0.358 |
|
| 2016 |
Goddard-Borger ED, Tysoe C, Withers SG. Glycosynthase mediated synthesis of psychosine. Carbohydrate Research. 435: 97-99. PMID 27721144 DOI: 10.1016/J.Carres.2016.09.013 |
0.663 |
|
| 2016 |
Nieto-Domínguez M, Prieto A, Fernández de Toro B, Cañada FJ, Barriuso J, Armstrong Z, Withers SG, de Eugenio LI, Martínez MJ. Enzymatic fine-tuning for 2-(6-hydroxynaphthyl) β-D-xylopyranoside synthesis catalyzed by the recombinant β-xylosidase BxTW1 from Talaromyces amestolkiae. Microbial Cell Factories. 15: 171. PMID 27716291 DOI: 10.1186/S12934-016-0568-6 |
0.36 |
|
| 2016 |
Zhang XF, Yang GY, Zhang Y, Xie Y, Withers SG, Feng Y. A general and efficient strategy for generating the stable enzymes. Scientific Reports. 6: 33797. PMID 27667190 DOI: 10.1038/Srep33797 |
0.433 |
|
| 2016 |
Ma F, Fischer M, Han Y, Withers SG, Feng Y, Yang GY. Substrate Engineering Enabling Fluorescence Droplet Entrapment for IVC-FACS Based Ultrahigh-Throughput Screening. Analytical Chemistry. PMID 27494801 DOI: 10.1021/Acs.Analchem.6B01712 |
0.337 |
|
| 2016 |
Santana AG, Vadlamani G, Mark BL, Withers SG. N-Acetyl glycals are tight-binding and environmentally insensitive inhibitors of hexosaminidases. Chemical Communications (Cambridge, England). 52: 7943-6. PMID 27253678 DOI: 10.1039/C6Cc02520J |
0.394 |
|
| 2016 |
Danby PM, Withers SG. Advances in Enzymatic Glycoside Synthesis. Acs Chemical Biology. PMID 27176929 DOI: 10.1021/Acschembio.6B00340 |
0.389 |
|
| 2016 |
Zoidl M, Gonzalez Santana A, Torvisco A, Tysoe C, Siriwardena A, Withers SG, Wrodnigg TM. The Staudinger/aza-Wittig/Grignard reaction as key step for the concise synthesis of 1-C-Alkyl-iminoalditol glycomimetics. Carbohydrate Research. PMID 27160850 DOI: 10.1016/J.Carres.2016.04.006 |
0.342 |
|
| 2016 |
Tysoe C, Williams LK, Keyzers R, Nguyen NT, Tarling C, Wicki J, Goddard-Borger ED, Aguda AH, Perry S, Foster LJ, Andersen RJ, Brayer GD, Withers SG. Potent Human α-Amylase Inhibition by the β-Defensin-like Protein Helianthamide. Acs Central Science. 2: 154-161. PMID 27066537 DOI: 10.1021/Acscentsci.5B00399 |
0.674 |
|
| 2016 |
Thonhofer M, Weber P, Gonzalez Santana A, Tysoe C, Fischer R, Pabst BM, Paschke E, Schalli M, Stütz AE, Tschernutter M, Windischhofer W, Withers SG. Synthesis of C-5a-substituted derivatives of 4-epi-isofagomine: notable β-galactosidase inhibitors and activity promotors of GM1-gangliosidosis related human lysosomal β-galactosidase mutant R201C. Carbohydrate Research. PMID 27063389 DOI: 10.1016/J.Carres.2016.03.020 |
0.341 |
|
| 2016 |
Caner S, Zhang X, Jiang J, Chen HM, Nguyen NT, Overkleeft H, Brayer GD, Withers SG. Glucosyl Epi-cyclophellitol Allows Mechanism- Based Inactivation and Structural Analysis of Human Pancreatic Alpha-Amylase. Febs Letters. PMID 27000970 DOI: 10.1002/1873-3468.12143 |
0.398 |
|
| 2016 |
Morgan JL, McNamara JT, Fischer M, Rich J, Chen HM, Withers SG, Zimmer J. Observing cellulose biosynthesis and membrane translocation in crystallo. Nature. PMID 26958837 DOI: 10.1038/Nature16966 |
0.311 |
|
| 2016 |
Thonhofer M, Weber P, Santana AG, Fischer R, Pabst BM, Paschke E, Schalli M, Stütz AE, Tschernutter M, Windischhofer W, Withers SG. Synthesis of C-5a-chain extended derivatives of 4-epi-isofagomine: Powerful β-galactosidase inhibitors and low concentration activators of GM1-gangliosidosis-related human lysosomal β-galactosidase. Bioorganic & Medicinal Chemistry Letters. PMID 26838810 DOI: 10.1016/J.Bmcl.2016.01.059 |
0.333 |
|
| 2016 |
Kwan DH, Jin Y, Jiang J, Chen HM, Kötzler MP, Overkleeft HS, Davies G, Withers SG. Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-β-glucosidases. Febs Letters. PMID 26790390 DOI: 10.1002/1873-3468.12059 |
0.753 |
|
| 2016 |
Chen HM, Armstrong Z, Hallam SJ, Withers SG. Synthesis and evaluation of a series of 6-chloro-4-methylumbelliferyl glycosides as fluorogenic reagents for screening metagenomic libraries for glycosidase activity. Carbohydrate Research. 421: 33-39. PMID 26774876 DOI: 10.1016/J.Carres.2015.12.010 |
0.342 |
|
| 2015 |
Thonhofer M, Gonzalez Santana A, Fischer R, Torvisco Gomez A, Saf R, Schalli M, Stütz AE, Withers SG. 5-Fluoro derivatives of 4-epi-isofagomine as d-galactosidase inhibitors and potential pharmacological chaperones for GM1-gangliosidosis as well as Fabry's disease. Carbohydrate Research. 420: 6-12. PMID 26717544 DOI: 10.1016/J.Carres.2015.10.009 |
0.339 |
|
| 2015 |
Mehr K, Withers SG. Mechanisms of the Sialidase and Trans-sialidase Activities of Bacterial Sialyltransferases from Glycosyltransferase Family 80 (GT80). Glycobiology. PMID 26582604 DOI: 10.1093/Glycob/Cwv105 |
0.447 |
|
| 2015 |
Kötzler MP, Withers SG. Proteolytic cleavage driven by glycosylation. The Journal of Biological Chemistry. PMID 26515062 DOI: 10.1074/Jbc.C115.698696 |
0.405 |
|
| 2015 |
Tai SS, Agafitei O, Gao Z, Liggins R, Petric M, Withers SG, Niikura M. Difluorosialic acids, potent novel influenza virus neuraminidase inhibitors, induce fewer drug resistance-associated neuraminidase mutations than does oseltamivir. Virus Research. 210: 126-132. PMID 26247418 DOI: 10.1016/J.Virusres.2015.07.015 |
0.701 |
|
| 2015 |
Williams LK, Zhang X, Caner S, Tysoe C, Nguyen NT, Wicki J, Williams DE, Coleman J, McNeill JH, Yuen V, Andersen RJ, Withers SG, Brayer GD. The amylase inhibitor montbretin A reveals a new glycosidase inhibition motif. Nature Chemical Biology. 11: 691-6. PMID 26214255 DOI: 10.1038/Nchembio.1865 |
0.37 |
|
| 2015 |
Volkers G, Worrall LJ, Kwan DH, Yu CC, Baumann L, Lameignere E, Wasney GA, Scott NE, Wakarchuk W, Foster LJ, Withers SG, Strynadka NC. Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation. Nature Structural & Molecular Biology. 22: 627-35. PMID 26192331 DOI: 10.1038/Nsmb.3060 |
0.713 |
|
| 2015 |
Kwan DH, Ernst S, Kötzler MP, Withers SG. Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes. Glycobiology. 25: 806-11. PMID 25964111 DOI: 10.1093/Glycob/Cwv031 |
0.711 |
|
| 2015 |
Kwan DH, Constantinescu I, Chapanian R, Higgins MA, Kötzler MP, Samain E, Boraston AB, Kizhakkedathu JN, Withers SG. Toward Efficient Enzymes for the Generation of Universal Blood through Structure-Guided Directed Evolution. Journal of the American Chemical Society. 137: 5695-705. PMID 25870881 DOI: 10.1021/Ja5116088 |
0.703 |
|
| 2015 |
Gesell A, Blaukopf M, Madilao L, Yuen MM, Withers SG, Mattsson J, Russell JH, Bohlmann J. The gymnosperm cytochrome P450 CYP750B1 catalyzes stereospecific monoterpene hydroxylation of (+)-sabinene in thujone biosynthesis in western redcedar. Plant Physiology. 168: 94-106. PMID 25829465 DOI: 10.1104/Pp.15.00315 |
0.372 |
|
| 2015 |
Yang GY, Li C, Fischer M, Cairo CW, Feng Y, Withers SG. A FRET probe for cell-based imaging of ganglioside-processing enzyme activity and high-throughput screening. Angewandte Chemie (International Ed. in English). 54: 5389-93. PMID 25757223 DOI: 10.1002/Anie.201411747 |
0.392 |
|
| 2015 |
Han YB, Wu L, Rich JR, Huang FT, Withers SG, Feng Y, Yang GY. Comprehensive characterization of sphingolipid ceramide N-deacylase for the synthesis and fatty acid remodeling of glycosphingolipids. Applied Microbiology and Biotechnology. 99: 6715-26. PMID 25683662 DOI: 10.1007/S00253-015-6421-8 |
0.379 |
|
| 2015 |
Sobhanifar S, Worrall LJ, Gruninger RJ, Wasney GA, Blaukopf M, Baumann L, Lameignere E, Solomonson M, Brown ED, Withers SG, Strynadka NC. Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase. Proceedings of the National Academy of Sciences of the United States of America. 112: E576-85. PMID 25624472 DOI: 10.1073/Pnas.1418084112 |
0.399 |
|
| 2015 |
Weck S, Robinson K, Smith MR, Withers SG. Understanding viral neuraminidase inhibition by substituted difluorosialic acids. Chemical Communications (Cambridge, England). 51: 2933-5. PMID 25587931 DOI: 10.1039/C4Cc08256G |
0.365 |
|
| 2015 |
Macdonald SS, Blaukopf M, Withers SG. N-acetylglucosaminidases from CAZy family GH3 are really glycoside phosphorylases, thereby explaining their use of histidine as an acid/base catalyst in place of glutamic acid. The Journal of Biological Chemistry. 290: 4887-95. PMID 25533455 DOI: 10.1074/Jbc.M114.621110 |
0.443 |
|
| 2015 |
Volkers G, Worrall LJ, Kwan DH, Yu CC, Baumann L, Lameignere E, Wasney GA, Scott NE, Wakarchuk W, Foster LJ, Withers SG, Strynadka NCJ. Structure of human ST8SiaIII sialyltransferase provides insight into cell-surface polysialylation Nature Structural and Molecular Biology. DOI: 10.1038/nsmb.3060 |
0.691 |
|
| 2014 |
Chapanian R, Kwan DH, Constantinescu I, Shaikh FA, Rossi NA, Withers SG, Kizhakkedathu JN. Enhancement of biological reactions on cell surfaces via macromolecular crowding. Nature Communications. 5: 4683. PMID 25140641 DOI: 10.1038/Ncomms5683 |
0.695 |
|
| 2014 |
Kwan DH, Withers SG. Periplasmic de-acylase helps bacteria don their biofilm coat. Proceedings of the National Academy of Sciences of the United States of America. 111: 10904-5. PMID 25006258 DOI: 10.1073/Pnas.1410789111 |
0.706 |
|
| 2014 |
Duo T, Goddard-Borger ED, Withers SG. Fluoro-glycosyl acridinones are ultra-sensitive active site titrating agents for retaining β-glycosidases. Chemical Communications (Cambridge, England). 50: 9379-82. PMID 25004867 DOI: 10.1039/C4Cc03299C |
0.67 |
|
| 2014 |
Syson K, Stevenson CE, Rashid AM, Saalbach G, Tang M, Tuukkanen A, Svergun DI, Withers SG, Lawson DM, Bornemann S. Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds α-maltose 1-phosphate and forms a maltosyl-enzyme intermediate. Biochemistry. 53: 2494-504. PMID 24689960 DOI: 10.1021/Bi500183C |
0.448 |
|
| 2014 |
Buchini S, Gallat FX, Greig IR, Kim JH, Wakatsuki S, Chavas LM, Withers SG. Tuning mechanism-based inactivators of neuraminidases: mechanistic and structural insights. Angewandte Chemie (International Ed. in English). 53: 3382-6. PMID 24591206 DOI: 10.1002/Anie.201309675 |
0.373 |
|
| 2014 |
Jongkees SA, Yoo H, Withers SG. Mechanistic investigations of unsaturated glucuronyl hydrolase from Clostridium perfringens. The Journal of Biological Chemistry. 289: 11385-95. PMID 24573682 DOI: 10.1074/Jbc.M113.545293 |
0.44 |
|
| 2014 |
Tysoe C, Withers SG. Fluorinated mechanism-based inhibitors: common themes and recent developments. Current Topics in Medicinal Chemistry. 14: 865-74. PMID 24484426 DOI: 10.2174/1568026614666140202204602 |
0.368 |
|
| 2014 |
Knott BC, Haddad Momeni M, Crowley MF, Mackenzie LF, Götz AW, Sandgren M, Withers SG, Ståhlberg J, Beckham GT. The mechanism of cellulose hydrolysis by a two-step, retaining cellobiohydrolase elucidated by structural and transition path sampling studies. Journal of the American Chemical Society. 136: 321-9. PMID 24341799 DOI: 10.1021/Ja410291U |
0.418 |
|
| 2014 |
Jongkees SA, Yoo H, Withers SG. Mechanistic insights from substrate preference in unsaturated glucuronyl hydrolase. Chembiochem : a European Journal of Chemical Biology. 15: 124-34. PMID 24227702 DOI: 10.1002/Cbic.201300547 |
0.414 |
|
| 2014 |
Yu CC, Hill T, Kwan DH, Chen HM, Lin CC, Wakarchuk W, Withers SG. A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis. Analytical Biochemistry. 444: 67-74. PMID 24121013 DOI: 10.1016/J.Ab.2013.09.030 |
0.715 |
|
| 2014 |
Jongkees SA, Withers SG. Unusual enzymatic glycoside cleavage mechanisms. Accounts of Chemical Research. 47: 226-35. PMID 23957528 DOI: 10.1021/Ar4001313 |
0.417 |
|
| 2014 |
Caner S, Nguyen N, Aguda A, Zhang R, Pan Y, Withers S, Brayer G. Mycobacterial Trehalose Synthase as a potential drug target for tuberculosis Acta Crystallographica Section a Foundations and Advances. 70: C441-C441. DOI: 10.1107/S2053273314095588 |
0.421 |
|
| 2013 |
Yu CC, Huang LD, Kwan DH, Wakarchuk WW, Withers SG, Lin CC. A glyco-gold nanoparticle based assay for α-2,8-polysialyltransferase from Neisseria meningitidis. Chemical Communications (Cambridge, England). 49: 10166-8. PMID 24051967 DOI: 10.1039/C3Cc45147J |
0.676 |
|
| 2013 |
Bie H, Yin J, He X, Kermode AR, Goddard-Borger ED, Withers SG, James MN. Insights into mucopolysaccharidosis I from the structure and action of α-L-iduronidase. Nature Chemical Biology. 9: 739-45. PMID 24036510 DOI: 10.1038/Nchembio.1357 |
0.683 |
|
| 2013 |
Mewis K, Armstrong Z, Song YC, Baldwin SA, Withers SG, Hallam SJ. Biomining active cellulases from a mining bioremediation system. Journal of Biotechnology. 167: 462-71. PMID 23906845 DOI: 10.1016/J.Jbiotec.2013.07.015 |
0.334 |
|
| 2013 |
Shaikh FA, Lammerts van Bueren A, Davies GJ, Withers SG. Identifying the catalytic acid/base in GH29 α-L-fucosidase subfamilies. Biochemistry. 52: 5857-64. PMID 23883131 DOI: 10.1021/Bi400183Q |
0.416 |
|
| 2013 |
Armstrong Z, Withers SG. Synthesis of glycans and glycopolymers through engineered enzymes. Biopolymers. 99: 666-74. PMID 23821499 DOI: 10.1002/Bip.22335 |
0.331 |
|
| 2013 |
Caner S, Nguyen N, Aguda A, Zhang R, Pan YT, Withers SG, Brayer GD. The structure of the Mycobacterium smegmatis trehalose synthase reveals an unusual active site configuration and acarbose-binding mode. Glycobiology. 23: 1075-83. PMID 23735230 DOI: 10.1093/Glycob/Cwt044 |
0.432 |
|
| 2013 |
Ratananikom K, Choengpanya K, Tongtubtim N, Charoenrat T, Withers SG, Kongsaeree PT. Mutational analysis in the glycone binding pocket of Dalbergia cochinchinensis β-glucosidase to increase catalytic efficiency toward mannosides. Carbohydrate Research. 373: 35-41. PMID 23583451 DOI: 10.1016/J.Carres.2012.10.018 |
0.402 |
|
| 2013 |
Ludwiczek ML, D'Angelo I, Yalloway GN, Brockerman JA, Okon M, Nielsen JE, Strynadka NC, Withers SG, McIntosh LP. Strategies for modulating the pH-dependent activity of a family 11 glycoside hydrolase. Biochemistry. 52: 3138-56. PMID 23578322 DOI: 10.1021/Bi400034M |
0.349 |
|
| 2013 |
Kim JH, Resende R, Wennekes T, Chen HM, Bance N, Buchini S, Watts AG, Pilling P, Streltsov VA, Petric M, Liggins R, Barrett S, McKimm-Breschkin JL, Niikura M, Withers SG. Mechanism-based covalent neuraminidase inhibitors with broad-spectrum influenza antiviral activity. Science (New York, N.Y.). 340: 71-5. PMID 23429702 DOI: 10.1126/Science.1232552 |
0.336 |
|
| 2013 |
Thompson J, Pikis A, Rich J, Hall BG, Withers SG. α-Galacturonidase(s): a new class of Family 4 glycoside hydrolases with strict specificity and a unique CHEV active site motif. Febs Letters. 587: 799-803. PMID 23416295 DOI: 10.1016/J.Febslet.2013.02.004 |
0.388 |
|
| 2013 |
Rakic B, Rao FV, Freimann K, Wakarchuk W, Strynadka NC, Withers SG. Structure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: porcine ST3Gal-I. Glycobiology. 23: 536-45. PMID 23300007 DOI: 10.1093/Glycob/Cwt001 |
0.403 |
|
| 2013 |
Stocker BL, Jongkees SA, Win-Mason AL, Dangerfield EM, Withers SG, Timmer MS. The 'mirror-image' postulate as a guide to the selection and evaluation of pyrrolidines as α-l-fucosidase inhibitors. Carbohydrate Research. 367: 29-32. PMID 23295913 DOI: 10.1016/J.Carres.2012.11.011 |
0.311 |
|
| 2013 |
Chan PH, Cheung AH, Okon M, Chen HM, Withers SG, McIntosh LP. Investigating the structural dynamics of α-1,4-galactosyltransferase C from Neisseria meningitidis by nuclear magnetic resonance spectroscopy. Biochemistry. 52: 320-32. PMID 23259770 DOI: 10.1021/Bi301317D |
0.376 |
|
| 2013 |
James M, Bie H, Goddard-Borger E, He X, Kermode A, Withers S, Yin J. Insights into the mutations that cause mucopolysaccharidosis I (MPS I) based on the structure and mechanism of α-L-iduronidase Acta Crystallographica Section a Foundations of Crystallography. 69: s49-s49. DOI: 10.1107/S0108767313099571 |
0.629 |
|
| 2012 |
Vocadlo DJ, Withers SG. How to make a difference: mechanisms of protein and nucleic acid modifying enzymes. Current Opinion in Chemical Biology. 16: 461-4. PMID 23168221 DOI: 10.1016/J.Cbpa.2012.10.033 |
0.598 |
|
| 2012 |
Withers SG, Davies GJ. Glycobiology: The case of the missing base. Nature Chemical Biology. 8: 952-3. PMID 23103941 DOI: 10.1038/Nchembio.1117 |
0.335 |
|
| 2012 |
Williams LK, Li C, Withers SG, Brayer GD. Order and disorder: differential structural impacts of myricetin and ethyl caffeate on human amylase, an antidiabetic target. Journal of Medicinal Chemistry. 55: 10177-86. PMID 23050660 DOI: 10.1021/Jm301273U |
0.363 |
|
| 2012 |
Yip VL, Withers SG. Identification of Tyr241 as a key catalytic base in the family 4 glycoside hydrolase BglT from Thermotoga maritima. Biochemistry. 51: 8464-74. PMID 23025815 DOI: 10.1021/Bi301021U |
0.421 |
|
| 2012 |
Chan PH, Weissbach S, Okon M, Withers SG, McIntosh LP. Nuclear magnetic resonance spectral assignments of α-1,4-galactosyltransferase LgtC from Neisseria meningitidis: substrate binding and multiple conformational states. Biochemistry. 51: 8278-92. PMID 22992161 DOI: 10.1021/Bi3010279 |
0.378 |
|
| 2012 |
Shim JH, Chen HM, Rich JR, Goddard-Borger ED, Withers SG. Directed evolution of a β-glycosidase from Agrobacterium sp. to enhance its glycosynthase activity toward C3-modified donor sugars. Protein Engineering, Design & Selection : Peds. 25: 465-72. PMID 22893693 DOI: 10.1093/Protein/Gzs045 |
0.703 |
|
| 2012 |
Goddard-Borger ED, Withers SG. Natural product biosynthesis: Tackling tunicamycin. Nature Chemistry. 4: 520-1. PMID 22717432 DOI: 10.1038/Nchem.1391 |
0.607 |
|
| 2012 |
Pengthaisong S, Chen CF, Withers SG, Kuaprasert B, Ketudat Cairns JR. Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition. Carbohydrate Research. 352: 51-9. PMID 22418094 DOI: 10.1016/J.Carres.2012.02.013 |
0.336 |
|
| 2012 |
Michikawa M, Ichinose H, Momma M, Biely P, Jongkees S, Yoshida M, Kotake T, Tsumuraya Y, Withers SG, Fujimoto Z, Kaneko S. Structural and biochemical characterization of glycoside hydrolase family 79 β-glucuronidase from Acidobacterium capsulatum. The Journal of Biological Chemistry. 287: 14069-77. PMID 22367201 DOI: 10.1074/Jbc.M112.346288 |
0.395 |
|
| 2012 |
Goddard-Borger ED, Tropak MB, Yonekawa S, Tysoe C, Mahuran DJ, Withers SG. Rapid assembly of a library of lipophilic iminosugars via the thiol-ene reaction yields promising pharmacological chaperones for the treatment of Gaucher disease. Journal of Medicinal Chemistry. 55: 2737-45. PMID 22360565 DOI: 10.1021/Jm201633Y |
0.694 |
|
| 2012 |
Goddard-Borger ED, Sakaguchi K, Reitinger S, Watanabe N, Ito M, Withers SG. Mechanistic insights into the 1,3-xylanases: useful enzymes for manipulation of algal biomass. Journal of the American Chemical Society. 134: 3895-902. PMID 22296113 DOI: 10.1021/Ja211836T |
0.717 |
|
| 2012 |
Pengthaisong S, Withers SG, Kuaprasert B, Svasti J, Cairns JR. The role of the oligosaccharide binding cleft of rice BGlu1 in hydrolysis of cellooligosaccharides and in their synthesis by rice BGlu1 glycosynthase. Protein Science : a Publication of the Protein Society. 21: 362-72. PMID 22238157 DOI: 10.1002/Pro.2021 |
0.387 |
|
| 2011 |
Jongkees SA, Withers SG. Glycoside cleavage by a new mechanism in unsaturated glucuronyl hydrolases. Journal of the American Chemical Society. 133: 19334-7. PMID 22047074 DOI: 10.1021/Ja209067V |
0.388 |
|
| 2011 |
Win-Mason AL, Jongkees SA, Withers SG, Tyler PC, Timmer MS, Stocker BL. Stereoselective total synthesis of aminoiminohexitols via carbamate annulation. The Journal of Organic Chemistry. 76: 9611-21. PMID 22022891 DOI: 10.1021/Jo201151B |
0.338 |
|
| 2011 |
Fröhlich RF, Fantur K, Furneaux RH, Paschke E, Stütz AE, Wicki J, Withers SG, Wrodnigg TM. A fluorescent probe for GM1 gangliosidosis related β-galactosidase: N-(dansylamino)hexylaminocarbonylpentyl-1,5-dideoxy-1,5-imino-D-galactitol. Bioorganic & Medicinal Chemistry Letters. 21: 6872-5. PMID 21974950 DOI: 10.1016/J.Bmcl.2011.09.012 |
0.332 |
|
| 2011 |
Lee SS, Greig IR, Vocadlo DJ, McCarter JD, Patrick BO, Withers SG. Structural, mechanistic, and computational analysis of the effects of anomeric fluorines on anomeric fluoride departure in 5-fluoroxylosyl fluorides. Journal of the American Chemical Society. 133: 15826-9. PMID 21910446 DOI: 10.1021/Ja204829R |
0.558 |
|
| 2011 |
Lin LY, Rakic B, Chiu CP, Lameignere E, Wakarchuk WW, Withers SG, Strynadka NC. Structure and mechanism of the lipooligosaccharide sialyltransferase from Neisseria meningitidis. The Journal of Biological Chemistry. 286: 37237-48. PMID 21880735 DOI: 10.1074/Jbc.M111.249920 |
0.381 |
|
| 2011 |
Zhang R, Pan YT, He S, Lam M, Brayer GD, Elbein AD, Withers SG. Mechanistic analysis of trehalose synthase from Mycobacterium smegmatis. The Journal of Biological Chemistry. 286: 35601-9. PMID 21840994 DOI: 10.1074/Jbc.M111.280362 |
0.441 |
|
| 2011 |
Lee HJ, Lairson LL, Rich JR, Lameignere E, Wakarchuk WW, Withers SG, Strynadka NC. Structural and kinetic analysis of substrate binding to the sialyltransferase Cst-II from Campylobacter jejuni. The Journal of Biological Chemistry. 286: 35922-32. PMID 21832050 DOI: 10.1074/Jbc.M111.261172 |
0.701 |
|
| 2011 |
Goddard-Borger ED, Fiege B, Kwan EM, Withers SG. Glycosynthase-mediated assembly of xylanase substrates and inhibitors. Chembiochem : a European Journal of Chemical Biology. 12: 1703-11. PMID 21710681 DOI: 10.1002/Cbic.201100229 |
0.707 |
|
| 2011 |
Torpenholt S, Le Nours J, Christensen U, Jahn M, Withers S, Østergaard PR, Borchert TV, Poulsen JC, Leggio LL. Activity of three β-1,4-galactanases on small chromogenic substrates Carbohydrate Research. 346: 2028-2033. PMID 21696710 DOI: 10.1016/J.Carres.2011.05.017 |
0.347 |
|
| 2011 |
Fröhlich RF, Furneaux RH, Mahuran DJ, Saf R, Stütz AE, Tropak MB, Wicki J, Withers SG, Wrodnigg TM. 1-Deoxy-D-galactonojirimycins with dansyl capped N-substituents as β-galactosidase inhibitors and potential probes for GM1 gangliosidosis affected cell lines. Carbohydrate Research. 346: 1592-8. PMID 21645885 DOI: 10.1016/J.Carres.2011.05.010 |
0.317 |
|
| 2011 |
Kwan DH, Chen HM, Ratananikom K, Hancock SM, Watanabe Y, Kongsaeree PT, Samuels AL, Withers SG. Self-immobilizing fluorogenic imaging agents of enzyme activity. Angewandte Chemie (International Ed. in English). 50: 300-3. PMID 21184404 DOI: 10.1002/Anie.201005705 |
0.693 |
|
| 2011 |
Kwan DH, Withers SG. Toward efficient enzymatic glycan synthesis: Directed evolution and enzyme engineering Journal of Carbohydrate Chemistry. 30: 181-205. DOI: 10.1080/07328303.2011.604457 |
0.729 |
|
| 2010 |
Goddard-Borger ED, Wennekes T, Withers SG. Molecular probes: Getting lucky in the lysosome. Nature Chemical Biology. 6: 881-3. PMID 21079598 DOI: 10.1038/Nchembio.470 |
0.653 |
|
| 2010 |
Kim YW, Zhang R, Chen H, Withers SG. O-glycoligases, a new category of glycoside bond-forming mutant glycosidases, catalyse facile syntheses of isoprimeverosides. Chemical Communications (Cambridge, England). 46: 8725-7. PMID 21031187 DOI: 10.1039/C0Cc03168B |
0.321 |
|
| 2010 |
Schitter G, Steiner AJ, Pototschnig G, Scheucher E, Thonhofer M, Tarling CA, Withers SG, Fantur K, Paschke E, Mahuran DJ, Rigat BA, Tropak MB, Illaszewicz C, Saf R, Stütz AE, et al. Fluorous iminoalditols: a new family of glycosidase inhibitors and pharmacological chaperones. Chembiochem : a European Journal of Chemical Biology. 11: 2026-33. PMID 20715263 DOI: 10.1002/Cbic.201000192 |
0.37 |
|
| 2010 |
Yang G, Rich JR, Gilbert M, Wakarchuk WW, Feng Y, Withers SG. Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases. Journal of the American Chemical Society. 132: 10570-7. PMID 20662530 DOI: 10.1021/Ja104167Y |
0.379 |
|
| 2010 |
Pototschnig G, De Csáky CM, Burke JR, Schitter G, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. Synthesis and biological evaluation of novel biotin-iminoalditol conjugates. Bioorganic & Medicinal Chemistry Letters. 20: 4077-9. PMID 20610152 DOI: 10.1016/J.Bmcl.2010.05.084 |
0.324 |
|
| 2010 |
Morley TJ, Withers SG. Chemoenzymatic synthesis and enzymatic analysis of 8-modified cytidine monophosphate-sialic acid and sialyl lactose derivatives. Journal of the American Chemical Society. 132: 9430-7. PMID 20557044 DOI: 10.1021/Ja102644A |
0.365 |
|
| 2010 |
Phenix CP, Rempel BP, Colobong K, Doudet DJ, Adam MJ, Clarke LA, Withers SG. Imaging of enzyme replacement therapy using PET. Proceedings of the National Academy of Sciences of the United States of America. 107: 10842-7. PMID 20534487 DOI: 10.1073/Pnas.1003247107 |
0.349 |
|
| 2010 |
Schitter G, Scheucher E, Steiner AJ, Stütz AE, Thonhofer M, Tarling CA, Withers SG, Wicki J, Fantur K, Paschke E, Mahuran DJ, Rigat BA, Tropak M, Wrodnigg TM. Synthesis of lipophilic 1-deoxygalactonojirimycin derivatives as D-galactosidase inhibitors. Beilstein Journal of Organic Chemistry. 6: 21. PMID 20502610 DOI: 10.3762/Bjoc.6.21 |
0.347 |
|
| 2010 |
Fröhlich RF, Furneaux RH, Mahuran DJ, Rigat BA, Stütz AE, Tropak MB, Wicki J, Withers SG, Wrodnigg TM. 1-Deoxynojirimycins with dansyl capped N-substituents as probes for Morbus Gaucher affected cell lines. Carbohydrate Research. 345: 1371-6. PMID 20471633 DOI: 10.1016/J.Carres.2010.04.015 |
0.355 |
|
| 2010 |
Kittl R, Withers SG. New approaches to enzymatic glycoside synthesis through directed evolution. Carbohydrate Research. 345: 1272-9. PMID 20427037 DOI: 10.1016/J.Carres.2010.04.002 |
0.426 |
|
| 2010 |
Macdonald JM, Tarling CA, Taylor EJ, Dennis RJ, Myers DS, Knapp S, Davies GJ, Withers SG. Chitinase inhibition by chitobiose and chitotriose thiazolines. Angewandte Chemie (International Ed. in English). 49: 2599-602. PMID 20209544 DOI: 10.1002/Anie.200906644 |
0.411 |
|
| 2010 |
Reitinger S, Yu Y, Wicki J, Ludwiczek M, D'Angelo I, Baturin S, Okon M, Strynadka NC, Lutz S, Withers SG, McIntosh LP. Circular permutation of Bacillus circulans xylanase: a kinetic and structural study. Biochemistry. 49: 2464-74. PMID 20163191 DOI: 10.1021/Bi100036F |
0.395 |
|
| 2010 |
Armstrong Z, Reitinger S, Kantner T, Withers SG. Enzymatic thioxyloside synthesis: characterization of thioglycoligase variants identified from a site-saturation mutagenesis library of Bacillus circulans xylanase. Chembiochem : a European Journal of Chemical Biology. 11: 533-8. PMID 20112321 DOI: 10.1002/Cbic.200900711 |
0.408 |
|
| 2010 |
KNAPP S, KIRK BA, VOCADLO D, WITHERS SG. ChemInform Abstract: An Allosamizoline/Glucosamine Hybrid NAGase Inhibitor. Cheminform. 28: no-no. DOI: 10.1002/chin.199742250 |
0.485 |
|
| 2010 |
Armstrong Z, Reitinger S, Kantner T, Withers SG. Cover Picture: Enzymatic Thioxyloside Synthesis: Characterization of Thioglycoligase Variants Identified from A Site-Saturation Mutagenesis Library ofBacillus CirculansXylanase (ChemBioChem 4/2010) Chembiochem. 11: 441-441. DOI: 10.1002/Cbic.201090010 |
0.318 |
|
| 2009 |
Steiner AJ, Schitter G, Stütz AE, Wrodnigg TM, Tarling CA, Withers SG, Mahuran DJ, Tropak MB. 2-Acetamino-1,2-dideoxynojirimycin-lysine hybrids as hexosaminidase inhibitors. Tetrahedron, Asymmetry. 20: 832-835. PMID 22328804 DOI: 10.1016/J.Tetasy.2009.02.015 |
0.345 |
|
| 2009 |
Chan PH, Lairson LL, Lee HJ, Wakarchuk WW, Strynadka NC, Withers SG, McIntosh LP. NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: histidine 188 is the general base. Biochemistry. 48: 11220-30. PMID 19824695 DOI: 10.1021/Bi901606N |
0.7 |
|
| 2009 |
Rao FV, Rich JR, Rakić B, Buddai S, Schwartz MF, Johnson K, Bowe C, Wakarchuk WW, Defrees S, Withers SG, Strynadka NC. Structural insight into mammalian sialyltransferases. Nature Structural & Molecular Biology. 16: 1186-8. PMID 19820709 DOI: 10.1038/Nsmb.1685 |
0.393 |
|
| 2009 |
Zhang R, Li C, Williams LK, Rempel BP, Brayer GD, Withers SG. Directed "in situ" inhibitor elongation as a strategy to structurally characterize the covalent glycosyl-enzyme intermediate of human pancreatic alpha-amylase. Biochemistry. 48: 10752-64. PMID 19803533 DOI: 10.1021/Bi901400P |
0.428 |
|
| 2009 |
Willis LM, Zhang R, Reid A, Withers SG, Wakarchuk WW. Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6. Biochemistry. 48: 10334-41. PMID 19788271 DOI: 10.1021/Bi9013825 |
0.46 |
|
| 2009 |
Yang G, Withers SG. Ultrahigh-throughput FACS-based screening for directed enzyme evolution. Chembiochem : a European Journal of Chemical Biology. 10: 2704-15. PMID 19780076 DOI: 10.1002/Cbic.200900384 |
0.387 |
|
| 2009 |
Kim JH, Ryu HW, Shim JH, Park KH, Withers SG. Development of new and selective Trypanosoma cruzi trans-sialidase inhibitors from sulfonamide chalcones and their derivatives. Chembiochem : a European Journal of Chemical Biology. 10: 2475-9. PMID 19780074 DOI: 10.1002/Cbic.200900108 |
0.311 |
|
| 2009 |
Shaikh FA, Randriantsoa M, Withers SG. Mechanistic analysis of the blood group antigen-cleaving endo-beta-galactosidase from Clostridium perfringens. Biochemistry. 48: 8396-404. PMID 19630404 DOI: 10.1021/Bi900991H |
0.418 |
|
| 2009 |
Hancock SM, Rich JR, Caines ME, Strynadka NC, Withers SG. Designer enzymes for glycosphingolipid synthesis by directed evolution. Nature Chemical Biology. 5: 508-14. PMID 19525967 DOI: 10.1038/Nchembio.191 |
0.38 |
|
| 2009 |
Lee HJ, Rakić B, Gilbert M, Wakarchuk WW, Withers SG, Strynadka NC. Structural and kinetic characterizations of the polysialic acid O-acetyltransferase OatWY from Neisseria meningitidis. The Journal of Biological Chemistry. 284: 24501-11. PMID 19525232 DOI: 10.1074/Jbc.M109.006049 |
0.439 |
|
| 2009 |
Morley TJ, Willis LM, Whitfield C, Wakarchuk WW, Withers SG. A new sialidase mechanism: bacteriophage K1F endo-sialidase is an inverting glycosidase. The Journal of Biological Chemistry. 284: 17404-10. PMID 19411257 DOI: 10.1074/Jbc.M109.003970 |
0.413 |
|
| 2009 |
Rempel BP, Withers SG. Non-Stick sugars: Synthesis of difluorosugar fluorides as potential glycosidase inactivators Australian Journal of Chemistry. 62: 590-599. DOI: 10.1071/Ch09223 |
0.392 |
|
| 2009 |
Rakić B, Withers SG. Recent developments in Glycoside Synthesis with Glycosynthases and Thioglycoligases Australian Journal of Chemistry. 62: 510-520. DOI: 10.1071/Ch09059 |
0.395 |
|
| 2009 |
Steiner AJ, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. Synthesis and biological evaluation of 1,5-dideoxy-1,5-iminoxylitol-Aamino acid hybrids as xylosidase inhibitors Australian Journal of Chemistry. 62: 553-557. DOI: 10.1071/Ch09040 |
0.331 |
|
| 2009 |
Davies GJ, Withers SG, Vocadlo DJ. The chitopentaose complex of a mutant en egg-white lysozyme displays no distortion of the -1 sugar away from a 4C1 chair conformation Australian Journal of Chemistry. 62: 528-532. DOI: 10.1071/Ch09038 |
0.572 |
|
| 2009 |
Le QT, Lee CK, Kim YW, Lee SJ, Zhang R, Withers SG, Kim YR, Auh JH, Park KH. Amylolytically-resistant tapioca starch modified by combined treatment of branching enzyme and maltogenic amylase Carbohydrate Polymers. 75: 9-14. DOI: 10.1016/J.Carbpol.2008.06.001 |
0.363 |
|
| 2008 |
Greig IR, Zahariev F, Withers SG. Elucidating the nature of the Streptomyces plicatus beta-hexosaminidase-bound intermediate using ab initio molecular dynamics simulations. Journal of the American Chemical Society. 130: 17620-8. PMID 19053474 DOI: 10.1021/Ja805640C |
0.369 |
|
| 2008 |
Steiner AJ, Schitter G, Stütz AE, Wrodnigg TM, Tarling CA, Withers SG, Fantur K, Mahuran D, Paschke E, Tropak M. 1-Deoxygalactonojirimycin-lysine hybrids as potent D-galactosidase inhibitors. Bioorganic & Medicinal Chemistry. 16: 10216-20. PMID 18996021 DOI: 10.1016/J.Bmc.2008.10.054 |
0.327 |
|
| 2008 |
Caines ME, Zhu H, Vuckovic M, Willis LM, Withers SG, Wakarchuk WW, Strynadka NC. The structural basis for T-antigen hydrolysis by Streptococcus pneumoniae: a target for structure-based vaccine design. The Journal of Biological Chemistry. 283: 31279-83. PMID 18784084 DOI: 10.1074/Jbc.C800150200 |
0.351 |
|
| 2008 |
Kuntz DA, Tarling CA, Withers SG, Rose DR. Structural analysis of Golgi alpha-mannosidase II inhibitors identified from a focused glycosidase inhibitor screen. Biochemistry. 47: 10058-68. PMID 18759458 DOI: 10.1021/Bi8010785 |
0.434 |
|
| 2008 |
Hancock SM, Tarling CA, Withers SG. High-throughput screening of cell lysates for ganglioside synthesis. Analytical Biochemistry. 382: 48-54. PMID 18703011 DOI: 10.1016/J.Ab.2008.07.023 |
0.358 |
|
| 2008 |
Hekmat O, He S, Warren RA, Withers SG. A mechanism-based ICAT strategy for comparing relative expression and activity levels of glycosidases in biological systems. Journal of Proteome Research. 7: 3282-92. PMID 18563928 DOI: 10.1021/Pr7008302 |
0.353 |
|
| 2008 |
Lee YS, Lee MH, Lee HS, Lee SJ, Kim YW, Zhang R, Withers SG, Kim KS, Lee SJ, Park KH. Enzymatic synthesis of a selective inhibitor for alpha-glucosidases: alpha-acarviosinyl-(1-->9)-3-alpha-D-glucopyranosylpropen. Journal of Agricultural and Food Chemistry. 56: 5324-30. PMID 18553919 DOI: 10.1021/Jf703655K |
0.308 |
|
| 2008 |
Lairson LL, Henrissat B, Davies GJ, Withers SG. Glycosyltransferases: structures, functions, and mechanisms. Annual Review of Biochemistry. 77: 521-55. PMID 18518825 DOI: 10.1146/annurev.biochem.76.061005.092322 |
0.686 |
|
| 2008 |
Rempel BP, Withers SG. Covalent inhibitors of glycosidases and their applications in biochemistry and biology. Glycobiology. 18: 570-86. PMID 18499865 DOI: 10.1093/Glycob/Cwn041 |
0.4 |
|
| 2008 |
Amorelli B, Yang C, Rempel B, Withers SG, Knapp S. N-Acetylhexosaminidase inhibitory properties of C-1 homologated GlcNAc- and GalNAc-thiazolines. Bioorganic & Medicinal Chemistry Letters. 18: 2944-7. PMID 18406613 DOI: 10.1016/J.Bmcl.2008.03.067 |
0.341 |
|
| 2008 |
Zhang R, McCarter JD, Braun C, Yeung W, Brayer GD, Withers SG. Synthesis and testing of 2-deoxy-2,2-dihaloglycosides as mechanism-based inhibitors of alpha-glycosidases. The Journal of Organic Chemistry. 73: 3070-7. PMID 18345685 DOI: 10.1021/Jo702565Q |
0.408 |
|
| 2008 |
Wilkinson SM, Liew CW, Mackay JP, Salleh HM, Withers SG, McLeod MD. Escherichia coli glucuronylsynthase: an engineered enzyme for the synthesis of beta-glucuronides. Organic Letters. 10: 1585-8. PMID 18345681 DOI: 10.1021/Ol8002767 |
0.316 |
|
| 2008 |
Maurus R, Begum A, Williams LK, Fredriksen JR, Zhang R, Withers SG, Brayer GD. Alternative catalytic anions differentially modulate human alpha-amylase activity and specificity. Biochemistry. 47: 3332-44. PMID 18284212 DOI: 10.1021/Bi701652T |
0.396 |
|
| 2008 |
Damager I, Buchini S, Amaya MF, Buschiazzo A, Alzari P, Frasch AC, Watts A, Withers SG. Kinetic and mechanistic analysis of Trypanosoma cruzi trans-sialidase reveals a classical ping-pong mechanism with acid/base catalysis. Biochemistry. 47: 3507-12. PMID 18284211 DOI: 10.1021/Bi7024832 |
0.464 |
|
| 2008 |
Steiner AJ, Stütz AE, Wrodnigg TM, Tarling CA, Withers SG, Hermetter A, Schmidinger H. Glycosidase profiling with immobilised glycosidase-inhibiting iminoalditols-A proof-of-concept study Bioorganic and Medicinal Chemistry Letters. 18: 1922-1925. PMID 18281216 DOI: 10.1016/J.Bmcl.2008.01.124 |
0.334 |
|
| 2008 |
Newstead SL, Potter JA, Wilson JC, Xu G, Chien CH, Watts AG, Withers SG, Taylor GL. The structure of Clostridium perfringens NanI sialidase and its catalytic intermediates. The Journal of Biological Chemistry. 283: 9080-8. PMID 18218621 DOI: 10.1074/Jbc.M710247200 |
0.333 |
|
| 2008 |
Tarling CA, Woods K, Zhang R, Brastianos HC, Brayer GD, Andersen RJ, Withers SG. The search for novel human pancreatic alpha-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts. Chembiochem : a European Journal of Chemical Biology. 9: 433-8. PMID 18214874 DOI: 10.1002/Cbic.200700470 |
0.333 |
|
| 2008 |
Li C, Rydberg E, Williams L, Zhang R, Begum A, Withers S, Brayer D. Mechanistic role of the catalytic residue D300 in human pancreatic alpha-amylase Acta Crystallographica Section a Foundations of Crystallography. 64: C263-C263. DOI: 10.1107/S0108767308091563 |
0.788 |
|
| 2008 |
Lobsanov Y, Lairson L, Romero P, Sleno B, Yip P, Withers S, Herscovics A, Howell L. Mechanism of retaining glycosyltransferases: structure of Kre2p/Mnt1p in complex with a donor analog Acta Crystallographica Section a Foundations of Crystallography. 64: C276-C277. DOI: 10.1107/S0108767308091162 |
0.606 |
|
| 2008 |
Vocadlo DJ, Withers SG. Glycosidase-catalysed oligosaccharide synthesis Carbohydrates in Chemistry and Biology. 2: 723-844. DOI: 10.1002/9783527618255.Ch29 |
0.549 |
|
| 2007 |
Hekmat O, Florizone C, Kim YW, Eltis LD, Warren RA, Withers SG. Specificity fingerprinting of retaining beta-1,4-glycanases in the Cellulomonas fimi secretome using two fluorescent mechanism-based probes. Chembiochem : a European Journal of Chemical Biology. 8: 2125-32. PMID 17952884 DOI: 10.1002/Cbic.200700481 |
0.374 |
|
| 2007 |
Yip VL, Thompson J, Withers SG. Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4. Biochemistry. 46: 9840-52. PMID 17676871 DOI: 10.1021/Bi700536P |
0.418 |
|
| 2007 |
Kim YW, Chen HM, Kim JH, Müllegger J, Mahuran D, Withers SG. Thioglycoligase-based assembly of thiodisaccharides: Screening as β-galactosidase inhibitors Chembiochem. 8: 1495-1499. PMID 17661304 DOI: 10.1002/Cbic.200700263 |
0.385 |
|
| 2007 |
Chen HM, Withers SG. Syntheses of the 3- and 4-thio analogues of 4-nitrophenyl 2-acetamido-2-deoxy-β-d-gluco- and galactopyranoside Carbohydrate Research. 342: 2212-2222. PMID 17612513 DOI: 10.1016/J.Carres.2007.05.023 |
0.366 |
|
| 2007 |
Schubert M, Poon DK, Wicki J, Tarling CA, Kwan EM, Nielsen JE, Withers SG, McIntosh LP. Probing electrostatic interactions along the reaction pathway of a glycoside hydrolase: histidine characterization by NMR spectroscopy. Biochemistry. 46: 7383-95. PMID 17547373 DOI: 10.1021/Bi700249M |
0.377 |
|
| 2007 |
Chiu CP, Lairson LL, Gilbert M, Wakarchuk WW, Withers SG, Strynadka NC. Structural analysis of the alpha-2,3-sialyltransferase Cst-I from Campylobacter jejuni in apo and substrate-analogue bound forms. Biochemistry. 46: 7196-204. PMID 17518445 DOI: 10.1021/Bi602543D |
0.728 |
|
| 2007 |
Wicki J, Schloegl J, Tarling CA, Withers SG. Recruitment of both uniform and differential binding energy in enzymatic catalysis: Xylanases from families 10 and 11 Biochemistry. 46: 6996-7005. PMID 17503782 DOI: 10.1021/Bi700359E |
0.406 |
|
| 2007 |
Jamaluddin H, Tumbale P, Withers SG, Acharya KR, Brew K. Conformational Changes Induced by Binding UDP-2F-galactose to α-1,3 Galactosyltransferase- Implications for Catalysis Journal of Molecular Biology. 369: 1270-1281. PMID 17493636 DOI: 10.1016/J.Jmb.2007.04.012 |
0.438 |
|
| 2007 |
Caines ME, Hancock SM, Tarling CA, Wrodnigg TM, Stick RV, Stütz AE, Vasella A, Withers SG, Strynadka NC. The structural basis of glycosidase inhibition by five-membered iminocyclitols: the clan a glycoside hydrolase endoglycoceramidase as a model system. Angewandte Chemie (International Ed. in English). 46: 4474-6. PMID 17487923 DOI: 10.1002/Anie.200700268 |
0.569 |
|
| 2007 |
Shaikh FA, Müllegger J, He S, Withers SG. Identification of the catalytic nucleophile in Family 42 β-galactosidases by intermediate trapping and peptide mapping: YesZ from Bacillus subtilis Febs Letters. 581: 2441-2446. PMID 17485082 DOI: 10.1016/J.Febslet.2007.04.053 |
0.421 |
|
| 2007 |
Steiner AJ, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. Iminoalditol-amino acid hybrids: synthesis and evaluation as glycosidase inhibitors Carbohydrate Research. 342: 1850-1858. PMID 17442281 DOI: 10.1016/J.Carres.2007.03.024 |
0.339 |
|
| 2007 |
Hommalai G, Withers SG, Chuenchor W, Cairns JRK, Svasti J. Enzymatic synthesis of cello-oligosaccharides by rice BGlu1 β-glucosidase glycosynthase mutants Glycobiology. 17: 744-753. PMID 17405771 DOI: 10.1093/Glycob/Cwm039 |
0.413 |
|
| 2007 |
Wicki J, Williams SJ, Withers SG. Transition-state mimicry by glycosidase inhibitors: a critical kinetic analysis. Journal of the American Chemical Society. 129: 4530-1. PMID 17385869 DOI: 10.1021/Ja0707254 |
0.537 |
|
| 2007 |
Caines ME, Vaughan MD, Tarling CA, Hancock SM, Warren RA, Withers SG, Strynadka NC. Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the Apo and GM3 ganglioside-bound forms. The Journal of Biological Chemistry. 282: 14300-8. PMID 17329247 DOI: 10.1074/Jbc.M611455200 |
0.406 |
|
| 2007 |
Tropak MB, Blanchard JE, Withers SG, Brown ED, Mahuran D. High-Throughput Screening for Human Lysosomal β-N-Acetyl Hexosaminidase Inhibitors Acting as Pharmacological Chaperones Chemistry and Biology. 14: 153-164. PMID 17317569 DOI: 10.1016/J.Chembiol.2006.12.006 |
0.41 |
|
| 2007 |
Poon DK, Ludwiczek ML, Schubert M, Kwan EM, Withers SG, McIntosh LP. NMR spectroscopic characterization of a beta-(1,4)-glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate. Biochemistry. 46: 1759-70. PMID 17253772 DOI: 10.1021/Bi061694C |
0.448 |
|
| 2007 |
Lairson LL, Wakarchuk WW, Withers SG. Alternative donor substrates for inverting and retaining glycosyltransferases Chemical Communications. 365-367. PMID 17220972 DOI: 10.1039/B614636H |
0.673 |
|
| 2007 |
Krupa JC, Shaya D, Chi L, Linhardt RJ, Cygler M, Withers SG, Mort JS. Quantitative continuous assay for hyaluronan synthase. Analytical Biochemistry. 361: 218-25. PMID 17173853 DOI: 10.1016/J.Ab.2006.11.011 |
0.413 |
|
| 2007 |
Poon DK, Withers SG, McIntosh LP. Direct demonstration of the flexibility of the glycosylated proline-threonine linker in the Cellulomonas fimi Xylanase Cex through NMR spectroscopic analysis. The Journal of Biological Chemistry. 282: 2091-100. PMID 17121820 DOI: 10.1074/Jbc.M609670200 |
0.304 |
|
| 2007 |
Tesić M, Wicki J, Poon DK, Withers SG, Douglas DJ. Gas phase noncovalent protein complexes that retain solution binding properties: Binding of xylobiose inhibitors to the beta-1, 4 exoglucanase from cellulomonas fimi. Journal of the American Society For Mass Spectrometry. 18: 64-73. PMID 17005414 DOI: 10.1016/J.Jasms.2006.08.012 |
0.382 |
|
| 2007 |
Meloncelli PJ, Gloster TM, Money VA, Tarling CA, Davies GJ, Withers SG, Stick RV. D-glucosylated derivatives of isofagomine and noeuromycin and their potential as inhibitors of β-glycoside hydrolases Australian Journal of Chemistry. 60: 549-565. DOI: 10.1071/Ch07188 |
0.603 |
|
| 2006 |
Poon DK, Schubert M, Au J, Okon M, Withers SG, McIntosh LP. Unambiguous determination of the ionization state of a glycoside hydrolase active site lysine by 1H-15N heteronuclear correlation spectroscopy. Journal of the American Chemical Society. 128: 15388-9. PMID 17132001 DOI: 10.1021/Ja065766Z |
0.328 |
|
| 2006 |
Lairson LL, Watts AG, Wakarchuk WW, Withers SG. Using substrate engineering to harness enzymatic promiscuity and expand biological catalysis. Nature Chemical Biology. 2: 724-8. PMID 17057723 DOI: 10.1038/Nchembio828 |
0.703 |
|
| 2006 |
Yip VL, Withers SG. Family 4 glycosidases carry out efficient hydrolysis of thioglycosides by an alpha,beta-elimination mechanism. Angewandte Chemie (International Ed. in English). 45: 6179-82. PMID 16917793 DOI: 10.1002/Anie.200601421 |
0.302 |
|
| 2006 |
Hancock SM, Vaughan MD, Withers SG. Engineering of glycosidases and glycosyltransferases Current Opinion in Chemical Biology. 10: 509-519. PMID 16905354 DOI: 10.1016/J.Cbpa.2006.07.015 |
0.32 |
|
| 2006 |
Blair DE, Hekmat O, Schüttelkopf AW, Shrestha B, Tokuyasu K, Withers SG, Van Aalten DMF. Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum Biochemistry. 45: 9416-9426. PMID 16878976 DOI: 10.1021/Bi0606694 |
0.404 |
|
| 2006 |
Aharoni A, Thieme K, Chiu CP, Buchini S, Lairson LL, Chen H, Strynadka NC, Wakarchuk WW, Withers SG. High-throughput screening methodology for the directed evolution of glycosyltransferases. Nature Methods. 3: 609-14. PMID 16862135 DOI: 10.1038/Nmeth899 |
0.649 |
|
| 2006 |
Kim YW, Chen H, Kim JH, Withers SG. Catalytic properties of a mutant β-galactosidase from Xanthomonas manihotis engineered to synthesize galactosyl-thio-β-1,3 and -β-1,4-glycosides Febs Letters. 580: 4377-4381. PMID 16844121 DOI: 10.1016/J.Febslet.2006.06.095 |
0.358 |
|
| 2006 |
Gordon RD, Sivarajah P, Satkunarajah M, Ma D, Tarling CA, Vizitiu D, Withers SG, Rini JM. X-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues. Journal of Molecular Biology. 360: 67-79. PMID 16769084 DOI: 10.1016/J.Jmb.2006.04.058 |
0.387 |
|
| 2006 |
Mayer C, Vocadlo DJ, Mah M, Rupitz K, Stoll D, Warren RA, Withers SG. Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi. The Febs Journal. 273: 2929-41. PMID 16762038 DOI: 10.1111/J.1742-4658.2006.05308.X |
0.568 |
|
| 2006 |
Lemieux MJ, Mark BL, Cherney MM, Withers SG, Mahuran DJ, James MN. Crystallographic structure of human beta-hexosaminidase A: interpretation of Tay-Sachs mutations and loss of GM2 ganglioside hydrolysis. Journal of Molecular Biology. 359: 913-29. PMID 16698036 DOI: 10.1016/J.Jmb.2006.04.004 |
0.382 |
|
| 2006 |
Kim YW, Fox DT, Hekmat O, Kantner T, McIntosh LP, Warren RA, Withers SG. Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi. Organic & Biomolecular Chemistry. 4: 2025-32. PMID 16688347 DOI: 10.1039/B601667G |
0.41 |
|
| 2006 |
Wrodnigg TM, Stütz AE, Tarling CA, Withers SG. Fine tuning of β-glucosidase inhibitory activity in the 2,5-dideoxy-2,5-imino-d-mannitol (DMDP) system Carbohydrate Research. 341: 1717-1722. PMID 16616905 DOI: 10.1016/J.Carres.2006.03.010 |
0.33 |
|
| 2006 |
Rye CS, Matte A, Cygler M, Withers SG. An atypical approach identifies TYR234 as the key base catalyst in chondroitin AC lyase. Chembiochem : a European Journal of Chemical Biology. 7: 631-7. PMID 16521140 DOI: 10.1002/Cbic.200500428 |
0.781 |
|
| 2006 |
Yip VL, Withers SG. Breakdown of oligosaccharides by the process of elimination. Current Opinion in Chemical Biology. 10: 147-55. PMID 16495121 DOI: 10.1016/J.Cbpa.2006.02.005 |
0.389 |
|
| 2006 |
Greimel P, Häusler H, Lundt I, Rupitz K, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. Fluorescent glycosidase inhibiting 1,5-dideoxy-1,5-iminoalditols Bioorganic and Medicinal Chemistry Letters. 16: 2067-2070. PMID 16481162 DOI: 10.1016/J.Bmcl.2006.01.095 |
0.301 |
|
| 2006 |
Kim YW, Lovering AL, Chen H, Kantner T, McIntosh LP, Strynadka NC, Withers SG. Expanding the thioglycoligase strategy to the synthesis of alpha-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex. Journal of the American Chemical Society. 128: 2202-3. PMID 16478160 DOI: 10.1021/Ja057904A |
0.338 |
|
| 2006 |
Yip VL, Withers SG. Mechanistic analysis of the unusual redox-elimination sequence employed by Thermotoga maritima BglT: a 6-phospho-beta-glucosidase from glycoside hydrolase family 4. Biochemistry. 45: 571-80. PMID 16401086 DOI: 10.1021/Bi052054X |
0.377 |
|
| 2006 |
Williams SJ, Hekmat O, Withers SG. Synthesis and testing of mechanism-based protein-profiling probes for retaining endo-glycosidases. Chembiochem : a European Journal of Chemical Biology. 7: 116-24. PMID 16397879 DOI: 10.1002/Cbic.200500279 |
0.573 |
|
| 2006 |
Salleh HM, Müllegger J, Reid SP, Chan WY, Hwang J, Warren RA, Withers SG. Cloning and characterization of Thermotoga maritima beta-glucuronidase. Carbohydrate Research. 341: 49-59. PMID 16303119 DOI: 10.1016/J.Carres.2005.10.005 |
0.444 |
|
| 2006 |
Watts AG, Oppezzo P, Withers SG, Alzari PM, Buschiazzo A. Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase. The Journal of Biological Chemistry. 281: 4149-55. PMID 16298994 DOI: 10.1074/Jbc.M510677200 |
0.481 |
|
| 2006 |
Lundt I, Steiner AJ, Stütz AE, Tarling CA, Ully S, Withers SG, Wrodnigg TM. Fluorescently tagged iminoalditol glycosidase inhibitors as novel biological probes and diagnostics Bioorganic and Medicinal Chemistry. 14: 1737-1742. PMID 16274997 DOI: 10.1016/J.Bmc.2005.10.021 |
0.331 |
|
| 2006 |
Yip VL, Withers SG. Family 4 glycoside hydrolases are special: The first β-elimination mechanism amongst glycoside hydrolases Biocatalysis and Biotransformation. 24: 167-176. DOI: 10.1080/10242420500515926 |
0.359 |
|
| 2006 |
Tauss A, Steiner AJ, Stütz AE, Tarling CA, Withers SG, Wrodnigg TM. L-Idoseptanosides: Substrates of D-glucosidases? Tetrahedron Asymmetry. 17: 234-239. DOI: 10.1016/J.Tetasy.2005.12.007 |
0.341 |
|
| 2006 |
Mildvan AS, Xia Z, Azurmendi HF, Legler PM, Balfour MR, Lairson LL, Withers SG, Gabelli SB, Bianchet MA, Amzel LM. Hydrogen bonding in the mechanism of GDP-mannose mannosyl hydrolase Journal of Molecular Structure. 790: 160-167. DOI: 10.1016/J.Molstruc.2005.09.024 |
0.684 |
|
| 2006 |
Pabba J, Rempel BP, Withers SG, Vasella A. Synthesis of glycaro-1,5-lactams and tetrahydrotetrazolopyridine-5- carboxylates: Inhibitors of β-D-glucuronidase and α-L-iduronidase Helvetica Chimica Acta. 89: 635-666. DOI: 10.1002/Hlca.200690066 |
0.314 |
|
| 2005 |
Duncan G, Tarling CA, Bingle WH, Nomellini JF, Yamage M, Dorocicz IR, Withers SG, Smit J. Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus: fusion with the beta-1,4-glycanase (Cex) from the cellulolytic bacterium Cellulomonas fimi yields a robust, catalytically active product. Applied Biochemistry and Biotechnology. 127: 95-110. PMID 16258187 DOI: 10.1385/Abab:127:2:095 |
0.4 |
|
| 2005 |
Vocadlo DJ, Withers SG. Detailed comparative analysis of the catalytic mechanisms of beta-N-acetylglucosaminidases from families 3 and 20 of glycoside hydrolases. Biochemistry. 44: 12809-18. PMID 16171396 DOI: 10.1021/Bi051121K |
0.636 |
|
| 2005 |
Knapp S, Yang C, Pabbaraja S, Rempel B, Reid S, Withers SG. Synthesis and kinetic analysis of the N-acetylhexosaminidase inhibitor XylNAc-isofagomine. The Journal of Organic Chemistry. 70: 7715-20. PMID 16149804 DOI: 10.1021/Jo051117E |
0.305 |
|
| 2005 |
Hekmat O, Kim YW, Williams SJ, He S, Withers SG. Active-site peptide "fingerprinting" of glycosidases in complex mixtures by mass spectrometry. Discovery of a novel retaining beta-1,4-glycanase in Cellulomonas fimi. The Journal of Biological Chemistry. 280: 35126-35. PMID 16085650 DOI: 10.1074/Jbc.M508434200 |
0.553 |
|
| 2005 |
Xia Z, Azurmendi HF, Lairson LL, Withers SG, Gabelli SB, Bianchet MA, Amzel LM, Mildvan AS. Mutational, structural, and kinetic evidence for a dissociative mechanism in the GDP-mannose mannosyl hydrolase reaction. Biochemistry. 44: 8989-97. PMID 15966723 DOI: 10.1021/Bi050583V |
0.683 |
|
| 2005 |
Rempel BP, Clarke LA, Withers SG. A homology model for human α-L-iduronidase: Insights into human disease Molecular Genetics and Metabolism. 85: 28-37. PMID 15862278 DOI: 10.1016/J.Ymgme.2004.12.006 |
0.303 |
|
| 2005 |
Müllegger J, Jahn M, Chen HM, Warren RA, Withers SG. Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts. Protein Engineering, Design & Selection : Peds. 18: 33-40. PMID 15790578 DOI: 10.1093/Protein/Gzi003 |
0.347 |
|
| 2005 |
Li C, Begum A, Numao S, Park KH, Withers SG, Brayer GD. Acarbose rearrangement mechanism implied by the kinetic and structural analysis of human pancreatic alpha-amylase in complex with analogues and their elongated counterparts. Biochemistry. 44: 3347-57. PMID 15736945 DOI: 10.1021/Bi048334E |
0.703 |
|
| 2005 |
Maurus R, Begum A, Kuo HH, Racaza A, Numao S, Andersen C, Tams JW, Vind J, Overall CM, Withers SG, Brayer GD. Structural and mechanistic studies of chloride induced activation of human pancreatic alpha-amylase. Protein Science : a Publication of the Protein Society. 14: 743-55. PMID 15722449 DOI: 10.1110/Ps.041079305 |
0.687 |
|
| 2005 |
Vocadlo DJ, Withers SG. The chemical synthesis of 2-deoxy-2-fluorodisaccharide probes of the hen egg white lysozyme mechanism. Carbohydrate Research. 340: 379-88. PMID 15680592 DOI: 10.1016/J.Carres.2004.12.015 |
0.589 |
|
| 2005 |
Varrot A, Yip VL, Li Y, Rajan SS, Yang X, Anderson WF, Thompson J, Withers SG, Davies GJ. NAD+ and metal-ion dependent hydrolysis by family 4 glycosidases: structural insight into specificity for phospho-beta-D-glucosides. Journal of Molecular Biology. 346: 423-35. PMID 15670594 DOI: 10.1016/J.Jmb.2004.11.058 |
0.401 |
|
| 2005 |
Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC. Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate. The Journal of Biological Chemistry. 280: 2105-15. PMID 15501829 DOI: 10.1074/Jbc.M410468200 |
0.405 |
|
| 2005 |
Tarling CA, Withers SG. The synthesis of a series of modified mannotrisaccharides as probes of the enzymes involved in the early stages of mammalian complex N-glycan formation. Carbohydrate Research. 339: 2487-97. PMID 15476709 DOI: 10.1016/J.Carres.2004.08.007 |
0.365 |
|
| 2005 |
Wrodnigg TM, Diness F, Gruber C, Häusler H, Lundt I, Rupitz K, Steiner AJ, Stütz AE, Tarling CA, Withers SG, Wölfler H. Probing the aglycon binding site of a beta-glucosidase: a collection of C-1-modified 2,5-dideoxy-2,5-imino-D-mannitol derivatives and their structure-activity relationships as competitive inhibitors. Bioorganic & Medicinal Chemistry. 12: 3485-95. PMID 15186833 DOI: 10.1016/J.Bmc.2004.04.037 |
0.385 |
|
| 2005 |
Gordon RD, Sivarajah P, Satkunarajah M, Ma D, Vizitiu D, Tarling CA, Withers SG, Rini JM. Structural insights into GnT-I substrate recognition and specificity Acta Crystallographica Section a Foundations of Crystallography. 61: c260-c260. DOI: 10.1107/S0108767305088914 |
0.335 |
|
| 2005 |
Tauss A, Greimel P, Rupitz K, Steiner AJ, Stütz AE, Withers SG, Wrodnigg TM. Non-natural aldofuranosides as substrates of a β-glucosidase Tetrahedron: Asymmetry. 16: 159-165. DOI: 10.1016/J.Tetasy.2004.11.027 |
0.307 |
|
| 2004 |
Lairson LL, Withers SG. Mechanistic analogies amongst carbohydrate modifying enzymes. Chemical Communications (Cambridge, England). 2243-8. PMID 15489968 DOI: 10.1039/B406490A |
0.699 |
|
| 2004 |
Yip VL, Withers SG. Nature's many mechanisms for the degradation of oligosaccharides. Organic & Biomolecular Chemistry. 2: 2707-13. PMID 15455137 DOI: 10.1039/B408880H |
0.346 |
|
| 2004 |
Gloster TM, Macdonald JM, Tarling CA, Stick RV, Withers SG, Davies GJ. Structural, thermodynamic, and kinetic analyses of tetrahydrooxazine-derived inhibitors bound to beta-glucosidases. The Journal of Biological Chemistry. 279: 49236-42. PMID 15356002 DOI: 10.1074/Jbc.M407195200 |
0.646 |
|
| 2004 |
Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF. Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis. Structure (London, England : 1993). 12: 1619-29. PMID 15341727 DOI: 10.1016/J.Str.2004.06.020 |
0.397 |
|
| 2004 |
Gloster TM, Williams SJ, Roberts S, Tarling CA, Wicki J, Withers SG, Davies GJ. Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A. Chemical Communications (Cambridge, England). 1794-5. PMID 15306887 DOI: 10.1039/B405152A |
0.543 |
|
| 2004 |
Numao S, Damager I, Li C, Wrodnigg TM, Begum A, Overall CM, Brayer GD, Withers SG. In situ extension as an approach for identifying novel alpha-amylase inhibitors. The Journal of Biological Chemistry. 279: 48282-91. PMID 15304511 DOI: 10.1074/Jbc.M406804200 |
0.678 |
|
| 2004 |
Kim YW, Lee SS, Warren RA, Withers SG. Directed evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire. The Journal of Biological Chemistry. 279: 42787-93. PMID 15252054 DOI: 10.1074/Jbc.M406890200 |
0.385 |
|
| 2004 |
Yip VL, Varrot A, Davies GJ, Rajan SS, Yang X, Thompson J, Anderson WF, Withers SG. An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima. Journal of the American Chemical Society. 126: 8354-5. PMID 15237973 DOI: 10.1021/Ja047632W |
0.372 |
|
| 2004 |
Damager I, Numao S, Chen H, Brayer GD, Withers SG. Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase. Carbohydrate Research. 339: 1727-37. PMID 15220082 DOI: 10.1016/J.Carres.2004.05.019 |
0.687 |
|
| 2004 |
Watts AG, Withers SG. Glycosynthase-catalysed formation of modified polysaccharide microstructures . The Biochemical Journal. 380: e9-10. PMID 15182233 DOI: 10.1042/Bj20040633 |
0.351 |
|
| 2004 |
Amaya MF, Watts AG, Damager I, Wehenkel A, Nguyen T, Buschiazzo A, Paris G, Frasch AC, Withers SG, Alzari PM. Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase. Structure (London, England : 1993). 12: 775-84. PMID 15130470 DOI: 10.1016/J.Str.2004.02.036 |
0.417 |
|
| 2004 |
Lairson LL, Chiu CP, Ly HD, He S, Wakarchuk WW, Strynadka NC, Withers SG. Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue. The Journal of Biological Chemistry. 279: 28339-44. PMID 15075344 DOI: 10.1074/Jbc.M400451200 |
0.721 |
|
| 2004 |
Rye CS, Withers SG. The synthesis of a novel thio-linked disaccharide of chondroitin as a potential inhibitor of polysaccharide lyases. Carbohydrate Research. 339: 699-703. PMID 15013408 DOI: 10.1016/J.Carres.2003.12.011 |
0.788 |
|
| 2004 |
Jahn M, Chen H, Müllegger J, Marles J, Warren RA, Withers SG. Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis. Chemical Communications (Cambridge, England). 274-5. PMID 14740034 DOI: 10.1039/B313155F |
0.362 |
|
| 2004 |
Chiu CP, Watts AG, Lairson LL, Gilbert M, Lim D, Wakarchuk WW, Withers SG, Strynadka NC. Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog. Nature Structural & Molecular Biology. 11: 163-70. PMID 14730352 DOI: 10.1038/Nsmb720 |
0.712 |
|
| 2004 |
Tropak MB, Reid SP, Guiral M, Withers SG, Mahuran D. Pharmacological enhancement of beta-hexosaminidase activity in fibroblasts from adult Tay-Sachs and Sandhoff Patients. The Journal of Biological Chemistry. 279: 13478-87. PMID 14724290 DOI: 10.1074/Jbc.M308523200 |
0.343 |
|
| 2004 |
Sulzenbacher G, Bignon C, Nishimura T, Tarling CA, Withers SG, Henrissat B, Bourne Y. Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis. The Journal of Biological Chemistry. 279: 13119-28. PMID 14715651 DOI: 10.1074/Jbc.M313783200 |
0.396 |
|
| 2004 |
Yang JK, Yoon HJ, Ahn HJ, Lee BI, Pedelacq JD, Liong EC, Berendzen J, Laivenieks M, Vieille C, Zeikus GJ, Vocadlo DJ, Withers SG, Suh SW. Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase. Journal of Molecular Biology. 335: 155-65. PMID 14659747 DOI: 10.1016/J.Jmb.2003.10.026 |
0.606 |
|
| 2004 |
Lee SS, Yu S, Withers SG. Detailed dissection of a new mechanism for glycoside cleavage: alpha-1,4-glucan lyase. Biochemistry. 42: 13081-90. PMID 14596624 DOI: 10.1021/Bi035189G |
0.377 |
|
| 2004 |
Paal K, Ito M, Withers SG. Paenibacillus sp. TS12 glucosylceramidase: kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues. The Biochemical Journal. 378: 141-9. PMID 14561218 DOI: 10.1042/Bj20031028 |
0.354 |
|
| 2004 |
Watts AG, Withers SG. The synthesis of some mechanistic probes for sialic acid processing enzymes and the labeling of a sialidase from Trypanosoma rangeli Canadian Journal of Chemistry. 82: 1581-1588. DOI: 10.1139/V04-125 |
0.421 |
|
| 2004 |
Jahn M, Withers SG. New Approaches to Enzymatic Oligosaccharide Synthesis: Glycosynthases and Thioglycoligases Cheminform. 35. DOI: 10.1080/10242420310001614351 |
0.378 |
|
| 2003 |
Gibson RP, Tarling CA, Roberts S, Withers SG, Davies GJ. The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution. The Journal of Biological Chemistry. 279: 1950-5. PMID 14570926 DOI: 10.1074/Jbc.M307643200 |
0.377 |
|
| 2003 |
Tarling CA, He S, Sulzenbacher G, Bignon C, Bourne Y, Henrissat B, Withers SG. Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis. The Journal of Biological Chemistry. 278: 47394-9. PMID 12975375 DOI: 10.1074/Jbc.M306610200 |
0.429 |
|
| 2003 |
Numao S, Kuntz DA, Withers SG, Rose DR. Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates. The Journal of Biological Chemistry. 278: 48074-83. PMID 12960159 DOI: 10.1074/Jbc.M309249200 |
0.724 |
|
| 2003 |
Ducros VM, Tarling CA, Zechel DL, Brzozowski AM, Frandsen TP, von Ossowski I, Schülein M, Withers SG, Davies GJ. Anatomy of glycosynthesis: structure and kinetics of the Humicola insolens Cel7B E197A and E197S glycosynthase mutants. Chemistry & Biology. 10: 619-28. PMID 12890535 DOI: 10.1016/S1074-5521(03)00143-1 |
0.402 |
|
| 2003 |
Jahn M, Stoll D, Warren RA, Szabó L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG. Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides. Chemical Communications (Cambridge, England). 1327-9. PMID 12841226 DOI: 10.1039/B302380J |
0.324 |
|
| 2003 |
Nieman CE, Wong AW, He S, Clarke L, Hopwood JJ, Withers SG. Family 39 alpha-l-iduronidases and beta-D-xylosidases react through similar glycosyl-enzyme intermediates: identification of the human iduronidase nucleophile. Biochemistry. 42: 8054-65. PMID 12834357 DOI: 10.1021/Bi034293V |
0.574 |
|
| 2003 |
Watts AG, Damager I, Amaya ML, Buschiazzo A, Alzari P, Frasch AC, Withers SG. Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: tyrosine is the catalytic nucleophile. Journal of the American Chemical Society. 125: 7532-3. PMID 12812490 DOI: 10.1021/Ja0344967 |
0.398 |
|
| 2003 |
Varrot A, Tarling CA, Macdonald JM, Stick RV, Zechel DL, Withers SG, Davies GJ. Direct observation of the protonation state of an imino sugar glycosidase inhibitor upon binding. Journal of the American Chemical Society. 125: 7496-7. PMID 12812472 DOI: 10.1021/Ja034917K |
0.612 |
|
| 2003 |
Zechel DL, Reid SP, Stoll D, Nashiru O, Warren RA, Withers SG. Mechanism, mutagenesis, and chemical rescue of a beta-mannosidase from cellulomonas fimi. Biochemistry. 42: 7195-204. PMID 12795616 DOI: 10.1021/Bi034329J |
0.431 |
|
| 2003 |
Hekmat O, Tokuyasu K, Withers SG. Subsite structure of the endo-type chitin deacetylase from a deuteromycete, Colletotrichum lindemuthianum: an investigation using steady-state kinetic analysis and MS. The Biochemical Journal. 374: 369-80. PMID 12775215 DOI: 10.1042/Bj20030204 |
0.36 |
|
| 2003 |
Gloster T, Williams SJ, Tarling CA, Roberts S, Dupont C, Jodoin P, Shareck F, Withers SG, Davies GJ. A xylobiose-derived isofagomine lactam glycosidase inhibitor binds as its amide tautomer. Chemical Communications (Cambridge, England). 944-5. PMID 12744311 DOI: 10.1039/B301829F |
0.586 |
|
| 2003 |
Poon DK, Webster P, Withers SG, McIntosh LP. Characterizing the pH-dependent stability and catalytic mechanism of the family 11 xylanase from the alkalophilic Bacillus agaradhaerens. Carbohydrate Research. 338: 415-21. PMID 12559743 DOI: 10.1016/S0008-6215(02)00486-X |
0.316 |
|
| 2003 |
Wicki J, Rose DR, Withers SG. Trapping covalent intermediates on beta-glycosidases. Methods in Enzymology. 354: 84-105. PMID 12418218 DOI: 10.1016/S0076-6879(02)54007-6 |
0.442 |
|
| 2003 |
Mosi RM, Withers SG. Trapping of alpha-glycosidase intermediates. Methods in Enzymology. 354: 64-84. PMID 12418217 DOI: 10.1016/S0076-6879(02)54006-4 |
0.419 |
|
| 2002 |
Withers SG, Wakarchuk WW, Strynadka NC. One step closer to a sweet conclusion. Chemistry & Biology. 9: 1270-3. PMID 12498879 DOI: 10.1016/S1074-5521(02)00291-0 |
0.332 |
|
| 2002 |
Rye CS, Withers SG. Development of an assay and determination of kinetic parameters for chondroitin AC lyase using defined synthetic substrates. Analytical Biochemistry. 308: 77-82. PMID 12234466 DOI: 10.1016/S0003-2697(02)00223-3 |
0.786 |
|
| 2002 |
Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabó L, Stoll D, Withers SG, Davies GJ. Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state. Angewandte Chemie (International Ed. in English). 41: 2824-7. PMID 12203498 DOI: 10.1002/1521-3773(20020802)41:15<2824::Aid-Anie2824>3.0.Co;2-G |
0.316 |
|
| 2002 |
Rye CS, Withers SG. Elucidation of the mechanism of polysaccharide cleavage by chondroitin AC lyase from Flavobacterium heparinum. Journal of the American Chemical Society. 124: 9756-67. PMID 12175234 DOI: 10.1021/Ja020627C |
0.776 |
|
| 2002 |
Williams SJ, Mark BL, Vocadlo DJ, James MN, Withers SG. Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The Journal of Biological Chemistry. 277: 40055-65. PMID 12171933 DOI: 10.1074/Jbc.M206481200 |
0.725 |
|
| 2002 |
Vocadlo DJ, Wicki J, Rupitz K, Withers SG. A case for reverse protonation: identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum beta-xylosidase and detailed kinetic analysis of a site-directed mutant. Biochemistry. 41: 9736-46. PMID 12146939 DOI: 10.1021/Bi020078N |
0.665 |
|
| 2002 |
Vocadlo DJ, Wicki J, Rupitz K, Withers SG. Mechanism of Thermoanaerobacterium saccharolyticum beta-xylosidase: kinetic studies. Biochemistry. 41: 9727-35. PMID 12146938 DOI: 10.1021/Bi020077V |
0.619 |
|
| 2002 |
Rye CS, Withers SG. Synthesis and evaluation of potential inhibitors of chondroitin AC lyase from Flavobacterium heparinum. The Journal of Organic Chemistry. 67: 4505-12. PMID 12076149 DOI: 10.1021/Jo020089M |
0.789 |
|
| 2002 |
Tolborg JF, Petersen L, Jensen KJ, Mayer C, Jakeman DL, Warren RA, Withers SG. Solid-phase oligosaccharide and glycopeptide synthesis using glycosynthases. The Journal of Organic Chemistry. 67: 4143-9. PMID 12054949 DOI: 10.1021/Jo0163445 |
0.354 |
|
| 2002 |
Lee SS, Yu S, Withers SG. alpha-1,4-Glucan lyase performs a trans-elimination via a nucleophilic displacement followed by a syn-elimination. Journal of the American Chemical Society. 124: 4948-9. PMID 11982345 DOI: 10.1021/Ja0255610 |
0.38 |
|
| 2002 |
Chir J, Withers S, Wan C, Li Y. Identification of the two essential groups in the family 3 beta-glucosidase from Flavobacterium meningosepticum by labelling and tandem mass spectrometric analysis. Biochemical Journal. 365: 857-863. PMID 11978178 DOI: 10.1042/Bj20020186 |
0.365 |
|
| 2002 |
Ly HD, Lougheed B, Wakarchuk WW, Withers SG. Mechanistic studies of a retaining alpha-galactosyltransferase from Neisseria meningitidis. Biochemistry. 41: 5075-85. PMID 11955055 DOI: 10.1021/Bi012031S |
0.454 |
|
| 2002 |
Rydberg EH, Li C, Maurus R, Overall CM, Brayer GD, Withers SG. Mechanistic analyses of catalysis in human pancreatic alpha-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids. Biochemistry. 41: 4492-502. PMID 11914097 DOI: 10.1021/Bi011821Z |
0.826 |
|
| 2002 |
Agnihotri G, He S, Hong L, Dakoji S, Withers SG, Liu HW. A revised mechanism for the inactivation of bovine liver enoyl-CoA hydratase by (methylenecyclopropyl)formyl-CoA based on unexpected results with the C114A mutant. Biochemistry. 41: 1843-52. PMID 11827529 DOI: 10.1021/Bi0119363 |
0.397 |
|
| 2002 |
Numao S, Maurus R, Sidhu G, Wang Y, Overall CM, Brayer GD, Withers SG. Probing the role of the chloride ion in the mechanism of human pancreatic alpha-amylase. Biochemistry. 41: 215-25. PMID 11772019 DOI: 10.1021/Bi0115636 |
0.698 |
|
| 2002 |
Blanchard JE, Withers SG. Rapid screening of the aglycone specificity of glycosidases: applications to enzymatic synthesis of oligosaccharides. Chemistry & Biology. 8: 627-33. PMID 11451664 DOI: 10.1016/S1074-5521(01)00038-2 |
0.376 |
|
| 2002 |
Hermetter A, Scholze H, Stütz AE, Withers SG, Wrodnigg TM. Powerful probes for glycosidases: novel, fluorescently tagged glycosidase inhibitors. Bioorganic & Medicinal Chemistry Letters. 11: 1339-42. PMID 11392550 DOI: 10.1016/S0960-894X(01)00209-8 |
0.335 |
|
| 2002 |
Jakeman DL, Withers SG. On expanding the repertoire of glycosynthases: Mutant β-galactosidases forming β-(1,6)-linkages Canadian Journal of Chemistry. 80: 866-870. DOI: 10.1139/V02-077 |
0.396 |
|
| 2002 |
Mark BL, Vocadlo DJ, Zhao D, Knapp S, Withers SG, James MNG. Biochemical and structural assessment of the 1-N-azasugar GalNAc-isofagomine as a potent family 20 β-hexosaminidase inhibitor Acta Crystallographica Section a Foundations of Crystallography. 58: c106-c106. DOI: 10.1107/S0108767302089274 |
0.566 |
|
| 2002 |
Macdonald JM, Stick RV, Tilbrook DMG, Withers SG. Synthesis with glycosynthases: Cello-oligomers of isofagomine and a tetrahydrooxazine as cellulase inhibitors Australian Journal of Chemistry. 55: 747-752. DOI: 10.1071/Ch02165 |
0.646 |
|
| 2002 |
Williams SJ, Withers SG. Glycosynthases: Mutant Glycosidases for Glycoside Synthesis Australian Journal of Chemistry. 55: 3. DOI: 10.1071/Ch02005 |
0.569 |
|
| 2002 |
Häusler H, Rupitz K, Stütz AE, Withers SG. N-Alkylated Derivatives of 1,5-Dideoxy-1,5-iminoxylitol as Β-Xylosidaseand Β-Glucosidase Inhibitors Monatshefte F?R Chemie / Chemical Monthly. 133: 555-560. DOI: 10.1007/978-3-7091-6130-2_14 |
0.385 |
|
| 2001 |
Nashiru O, Zechel DL, Stoll D, Mohammadzadeh T, Warren RAJ, Withers SG. β-Mannosynthase: Synthesis of β-Mannosides with a Mutant β-Mannosidase. Angewandte Chemie (International Ed. in English). 40: 417-420. PMID 29712412 DOI: 10.1002/1521-3757(20010119)113:2<431::Aid-Ange431>3.0.Co;2-Z |
0.331 |
|
| 2001 |
Zechel DL, Withers SG. Dissection of nucleophilic and acid-base catalysis in glycosidases Current Opinion in Chemical Biology. 5: 643-649. PMID 11738173 DOI: 10.1016/S1367-5931(01)00260-5 |
0.332 |
|
| 2001 |
Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW. A structural view of the action of Escherichia coli (lacZ) beta-galactosidase. Biochemistry. 40: 14781-94. PMID 11732897 DOI: 10.1021/Bi011727I |
0.37 |
|
| 2001 |
Lee SS, He S, Withers SG. Identification of the catalytic nucleophile of the Family 31 alpha-glucosidase from Aspergillus niger via trapping of a 5-fluoroglycosyl-enzyme intermediate. The Biochemical Journal. 359: 381-6. PMID 11583585 DOI: 10.1042/0264-6021:3590381 |
0.389 |
|
| 2001 |
Mark BL, Vocadlo DJ, Zhao D, Knapp S, Withers SG, James MN. Biochemical and structural assessment of the 1-N-azasugar GalNAc-isofagomine as a potent family 20 beta-N-acetylhexosaminidase inhibitor. The Journal of Biological Chemistry. 276: 42131-7. PMID 11522797 DOI: 10.1074/Jbc.M107154200 |
0.633 |
|
| 2001 |
Vocadlo DJ, Davies GJ, Laine R, Withers SG. Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate. Nature. 412: 835-8. PMID 11518970 DOI: 10.1038/35090602 |
0.635 |
|
| 2001 |
Joshi MD, Sidhu G, Nielsen JE, Brayer GD, Withers SG, McIntosh LP. Dissecting the electrostatic interactions and pH-dependent activity of a family 11 glycosidase Biochemistry. 40: 10115-10139. PMID 11513590 DOI: 10.2210/Pdb1Hv0/Pdb |
0.36 |
|
| 2001 |
Blanchard JE, Gal L, He S, Foisy J, Warren RA, Withers SG. The identification of the catalytic nucleophiles of two beta-galactosidases from glycoside hydrolase family 35. Carbohydrate Research. 333: 7-17. PMID 11423106 DOI: 10.1016/S0008-6215(01)00108-2 |
0.355 |
|
| 2001 |
Mayer C, Jakeman DL, Mah M, Karjala G, Gal L, Warren RA, Withers SG. Directed evolution of new glycosynthases from Agrobacterium beta-glucosidase: a general screen to detect enzymes for oligosaccharide synthesis. Chemistry & Biology. 8: 437-43. PMID 11358691 DOI: 10.1016/S1074-5521(01)00022-9 |
0.409 |
|
| 2001 |
Hou Y, Vocadlo DJ, Leung A, Withers SG, Mahuran D. Characterization of the Glu and Asp residues in the active site of human beta-hexosaminidase B. Biochemistry. 40: 2201-9. PMID 11329289 DOI: 10.1021/Bi002018S |
0.617 |
|
| 2001 |
Wrodnigg TM, Withers SG, Stütz AE. Novel, lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol (DMDP) are powerful beta-glucosidase inhibitors. Bioorganic & Medicinal Chemistry Letters. 11: 1063-4. PMID 11327590 DOI: 10.1016/S0960-894X(01)00126-3 |
0.34 |
|
| 2001 |
Persson K, Ly HD, Dieckelmann M, Wakarchuk WW, Withers SG, Strynadka NC. Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs. Nature Structural Biology. 8: 166-75. PMID 11175908 DOI: 10.1038/84168 |
0.377 |
|
| 2001 |
Mark BL, Vocadlo DJ, Knapp S, Triggs-Raine BL, Withers SG, James MN. Crystallographic evidence for substrate-assisted catalysis in a bacterial beta-hexosaminidase. The Journal of Biological Chemistry. 276: 10330-7. PMID 11124970 DOI: 10.1074/Jbc.M011067200 |
0.606 |
|
| 2001 |
Arribas JC, Herrero AG, Martín-Lomas M, Cañada FJ, He S, Withers SG. Differential mechanism-based labeling and unequivocal activity assignment of the two active sites of intestinal lactase/phlorizin hydrolase. European Journal of Biochemistry. 267: 6996-7005. PMID 11106409 DOI: 10.1046/J.1432-1327.2000.01784.X |
0.362 |
|
| 2001 |
Wong AW, He S, Withers SG. Synthesis of 5-fluoro-β-D-glucopyranosyluronic acid fluoride and its evaluation as a mechanistic probe of Escherichia coli β-glucuronidase Canadian Journal of Chemistry. 79: 510-518. DOI: 10.1139/V00-155 |
0.581 |
|
| 2001 |
Withers S. Mechanisms of glycosyl transferases and hydrolases Carbohydrate Polymers. 44: 325-337. DOI: 10.1016/S0144-8617(00)00249-6 |
0.444 |
|
| 2001 |
Adam MJ, Wong AW, Withers SG. Synthesis of 2,6-dideoxy-2-fluoro-6-[18F]-fluoro-β-D-glucopyranosyl fluoride (2,6FGF) as a potential imaging probe for glucocerebrosidase Journal of Labelled Compounds and Radiopharmaceuticals. 44: S448-S450. DOI: 10.1002/Jlcr.25804401158 |
0.526 |
|
| 2001 |
Vocadlo DJ, Withers SG. ChemInform Abstract: Glycosidase-Catalyzed Oligosaccharide Synthesis Cheminform. 32: no-no. DOI: 10.1002/chin.200112257 |
0.51 |
|
| 2000 |
Ly HD, Howard S, Shum K, He S, Zhu A, Withers SG. The synthesis, testing and use of 5-fluoro-alpha-D-galactosyl fluoride to trap an intermediate on green coffee bean alpha-galactosidase and identify the catalytic nucleophile. Carbohydrate Research. 329: 539-47. PMID 11128583 DOI: 10.1016/S0008-6215(00)00214-7 |
0.433 |
|
| 2000 |
Numao S, He S, Evjen G, Howard S, Tollersrud OK, Withers SG. Identification of Asp197 as the catalytic nucleophile in the family 38 alpha-mannosidase from bovine kidney lysosomes. Febs Letters. 484: 175-8. PMID 11078873 DOI: 10.1016/S0014-5793(00)02148-7 |
0.689 |
|
| 2000 |
Rye CS, Withers SG. Glycosidase mechanisms. Current Opinion in Chemical Biology. 4: 573-80. PMID 11006547 DOI: 10.1016/S1367-5931(00)00135-6 |
0.751 |
|
| 2000 |
Notenboom V, Williams SJ, Hoos R, Withers SG, Rose DR. Detailed structural analysis of glycosidase/inhibitor interactions: complexes of Cex from Cellulomonas fimi with xylobiose-derived aza-sugars. Biochemistry. 39: 11553-63. PMID 10995222 DOI: 10.1021/Bi0010625 |
0.573 |
|
| 2000 |
Williams SJ, Withers SG. Glycosyl fluorides in enzymatic reactions. Carbohydrate Research. 327: 27-46. PMID 10968675 DOI: 10.1016/S0008-6215(00)00041-0 |
0.615 |
|
| 2000 |
Vocadlo DJ, Withers SG. Identification of active site residues in glycosidases by use of tandem mass spectrometry. Methods in Molecular Biology (Clifton, N.J.). 146: 203-22. PMID 10948504 DOI: 10.1385/1-59259-045-4:203 |
0.551 |
|
| 2000 |
Hart DO, He S, Chany CJ, Withers SG, Sims PF, Sinnott ML, Brumer H. Identification of Asp-130 as the catalytic nucleophile in the main alpha-galactosidase from Phanerochaete chrysosporium, a family 27 glycosyl hydrolase. Biochemistry. 39: 9826-36. PMID 10933800 DOI: 10.1021/Bi0008074 |
0.645 |
|
| 2000 |
Ly HD, Withers SG. Mutagenesis of glycosidases. Annual Review of Biochemistry. 68: 487-522. PMID 10872458 DOI: 10.1146/Annurev.Biochem.68.1.487 |
0.398 |
|
| 2000 |
Joshi MD, Sidhu G, Pot I, Brayer GD, Withers SG, McIntosh LP. Hydrogen bonding and catalysis: A novel explanation for how a single amino acid substitution can change the pH optimum of a glycosidase Journal of Molecular Biology. 299: 255-279. PMID 10860737 DOI: 10.1006/Jmbi.2000.3722 |
0.407 |
|
| 2000 |
Hou Y, Vocadlo D, Withers S, Mahuran D. Role of beta Arg211 in the active site of human beta-hexosaminidase B. Biochemistry. 39: 6219-27. PMID 10821697 DOI: 10.1021/Bi992464J |
0.642 |
|
| 2000 |
Brayer GD, Sidhu G, Maurus R, Rydberg EH, Braun C, Wang Y, Nguyen NT, Overall CM, Withers SG. Subsite mapping of the human pancreatic alpha-amylase active site through structural, kinetic, and mutagenesis techniques. Biochemistry. 39: 4778-91. PMID 10769135 DOI: 10.1021/Bi9921182 |
0.822 |
|
| 2000 |
Connelly GP, Withers SG, McIntosh LP. Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate Protein Science. 9: 512-524. PMID 10752613 DOI: 10.1110/Ps.9.3.512 |
0.345 |
|
| 2000 |
Dan S, Marton I, Dekel M, Bravdo BA, He S, Withers SG, Shoseyov O. Cloning, expression, characterization, and nucleophile identification of family 3, Aspergillus niger β-glucosidase Journal of Biological Chemistry. 275: 4973-4980. PMID 10671536 DOI: 10.1074/Jbc.275.7.4973 |
0.409 |
|
| 2000 |
Mayer C, Zechel DL, Reid SP, Warren RAJ, Withers SG. The E358S mutant of Agrobacterium sp. β-glucosidase is a greatly improved glycosynthase Febs Letters. 466: 40-44. PMID 10648808 DOI: 10.1016/S0014-5793(99)01751-2 |
0.347 |
|
| 2000 |
Zechel DL, Withers SG. Glycosidase mechanisms: Anatomy of a finely tuned catalyst Accounts of Chemical Research. 33: 11-18. PMID 10639071 DOI: 10.1021/Ar970172+ |
0.422 |
|
| 2000 |
Vocadlo DJ, Mayer C, He S, Withers SG. Mechanism of action and identification of Asp242 as the catalytic nucleophile of Vibrio furnisii N-acetyl-beta-D-glucosaminidase using 2-acetamido-2-deoxy-5-fluoro-alpha-L-idopyranosyl fluoride. Biochemistry. 39: 117-26. PMID 10625486 DOI: 10.1021/Bi991958D |
0.634 |
|
| 2000 |
Lougheed B, Ly HD, Wakarchuk WW, Withers SG. Glycosyl fluorides can function as substrates for nucleotide phosphosugar-dependent glycosyltransferases. The Journal of Biological Chemistry. 274: 37717-22. PMID 10608830 DOI: 10.1074/Jbc.274.53.37717 |
0.44 |
|
| 2000 |
Wrodnigg TM, Gaderbauer W, Greimel P, Häusler H, Sprenger FK(, Stütz AE, Virgona C, Withers SG. Biologically Active 1-Aminodeoxy and 1-O-Alkyl Derivatives of The Powerful D-Glucosidase Inhibitor 2,5-Dideoxy-2,5-Imino-D-Mannitol Journal of Carbohydrate Chemistry. 19: 975-990. DOI: 10.1080/07328300008544129 |
0.362 |
|
| 2000 |
Fairweather JK, Stick RV, Withers SG. A 'glycosynthase'-assisted synthesis of some epoxyalkyl β-C-oligosaccharides Australian Journal of Chemistry. 53: 913-916. DOI: 10.1071/Ch00125 |
0.566 |
|
| 2000 |
STOLL D, HE S, WITHERS SG, WARREN RAJ. Identification of Glu-519 as the catalytic nucleophile in β-mannosidase 2A from Cellulomonas fimi Biochemical Journal. 351: 833. DOI: 10.1042/Bj3510833 |
0.407 |
|
| 2000 |
Williams SJ, Hoos R, Withers SG. Nanomolar versus Millimolar Inhibition by Xylobiose-Derived Azasugars: Significant Differences between Two Structurally Distinct Xylanases Journal of the American Chemical Society. 122: 2223-2235. DOI: 10.1021/Ja993805J |
0.574 |
|
| 2000 |
Namchuk MN, McCarter JD, Becalski A, Andrews T, Withers SG. The Role of Sugar Substituents in Glycoside Hydrolysis Journal of the American Chemical Society. 122: 1270-1277. DOI: 10.1021/Ja992044H |
0.328 |
|
| 2000 |
Williams SJ, Notenboom V, Wicki J, Rose DR, Withers SG. A New, Simple, High-Affinity Glycosidase Inhibitor: Analysis of Binding through X-ray Crystallography, Mutagenesis, and Kinetic Analysis Journal of the American Chemical Society. 122: 4229-4230. DOI: 10.1021/Ja0002870 |
0.504 |
|
| 1999 |
Uitdehaag JCM, Mosi R, Kalk KH, Van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW. X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the α-amylase family Nature Structural Biology. 6: 432-436. PMID 10331869 DOI: 10.1038/8235 |
0.39 |
|
| 1999 |
Sidhu G, Withers SG, Nguyen NT, McIntosh LP, Ziser L, Brayer GD. Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase Biochemistry. 38: 5346-5354. PMID 10220321 DOI: 10.1021/Bi982946F |
0.436 |
|
| 1999 |
Sulzenbacher G, Mackenzie LF, Wilson KS, Withers SG, Dupont C, Davies GJ. The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 A resolution. Biochemistry. 38: 4826-33. PMID 10200171 DOI: 10.1021/Bi982648I |
0.378 |
|
| 1999 |
Rydberg EH, Sidhu G, Vo HC, Hewitt J, Côte HC, Wang Y, Numao S, MacGillivray RT, Overall CM, Brayer GD, Withers SG. Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris. Protein Science : a Publication of the Protein Society. 8: 635-43. PMID 10091666 DOI: 10.1110/Ps.8.3.635 |
0.804 |
|
| 1999 |
Withers SG. 1998 Hoffmann La Roche Award Lecture Understanding and exploiting glycosidases Canadian Journal of Chemistry. 77: 1-11. DOI: 10.1139/V98-235 |
0.458 |
|
| 1999 |
MOSI R, WITHERS SG. Synthesis and kinetic evaluation of 4-deoxymaltopentaose and 4-deoxymaltohexaose as inhibitors of muscle and potato α-glucan phosphorylases Biochemical Journal. 338: 251-256. DOI: 10.1042/Bj3380251 |
0.327 |
|
| 1998 |
Mosi R, Sham H, Uitdehaag JCM, Ruiterkamp R, Dijkstra BW, Withers SG. Reassessment of acarbose as a transition state analogue inhibitor of cyclodextrin glycosyltransferase Biochemistry. 37: 17192-17198. PMID 9860832 DOI: 10.1021/Bi981109A |
0.413 |
|
| 1998 |
Wong AW, He S, Grubb JH, Sly WS, Withers SG. Identification of Glu-540 as the catalytic nucleophile of human beta-glucuronidase using electrospray mass spectrometry. The Journal of Biological Chemistry. 273: 34057-62. PMID 9852062 DOI: 10.1074/Jbc.273.51.34057 |
0.606 |
|
| 1998 |
Zechel DL, He S, Dupont C, Withers SG. Identification of glu-120 as the catalytic nucleophile in streptomyces lividans endoglucanase CelB Biochemical Journal. 336: 139-145. PMID 9806895 DOI: 10.1042/Bj3360139 |
0.347 |
|
| 1998 |
Vocadlo DJ, MacKenzie LF, He S, Zeikus GJ, Withers SG. Identification of glu-277 as the catalytic nucleophile of Thermoanaerobacterium saccharolyticum beta-xylosidase using electrospray MS. The Biochemical Journal. 335: 449-55. PMID 9761746 DOI: 10.1042/Bj3350449 |
0.619 |
|
| 1998 |
MacKenzie LF, Sulzenbacher G, Divne C, Jones TA, Wöldike HF, Schülein M, Withers SG, Davies GJ. Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate. The Biochemical Journal. 409-16. PMID 9761741 DOI: 10.1042/Bj3350409 |
0.456 |
|
| 1998 |
Notenboom V, Birsan C, Nitz M, Rose DR, Warren RA, Withers SG. Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants. Nature Structural Biology. 5: 812-8. PMID 9731776 DOI: 10.1038/1852 |
0.324 |
|
| 1998 |
Davies GJ, Mackenzie L, Varrot A, Dauter M, Brzozowski AM, Schülein M, Withers SG. Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase. Biochemistry. 37: 11707-13. PMID 9718293 DOI: 10.1021/Bi981315I |
0.448 |
|
| 1998 |
Zhu J, Withers SG, Reichardt PB, Treadwell E, Clausen TP. Salicortin: a repeat-attack new-mechanism-based Agrobacterium faecalis beta-glucosidase inhibitor. The Biochemical Journal. 367-71. PMID 9601065 DOI: 10.1042/Bj3320367 |
0.445 |
|
| 1998 |
Zechel DL, Konermann L, Withers SG, Douglas DJ. Pre-steady state kinetic analysis of an enzymatic reaction monitored by time-resolved electrospray ionization mass spectrometry Biochemistry. 37: 7664-7669. PMID 9601025 DOI: 10.1021/Bi980445O |
0.35 |
|
| 1998 |
Notenboom V, Birsan C, Warren RA, Withers SG, Rose DR. Exploring the cellulose/xylan specificity of the beta-1,4-glycanase cex from Cellulomonas fimi through crystallography and mutation. Biochemistry. 37: 4751-8. PMID 9537990 DOI: 10.1021/Bi9729211 |
0.415 |
|
| 1998 |
Howard S, Withers SG. Labeling and identification of the postulated acid/base catalyst in the alpha-glucosidase from Saccharomyces cerevisiae using a novel bromoketone C-glycoside. Biochemistry. 37: 3858-64. PMID 9521706 DOI: 10.1021/Bi9727085 |
0.384 |
|
| 1998 |
Lawson SL, Warren RA, Withers SG. Mechanistic consequences of replacing the active-site nucleophile Glu-358 in Agrobacterium sp. beta-glucosidase with a cysteine residue. The Biochemical Journal. 330: 203-9. PMID 9461511 DOI: 10.1042/Bj3300203 |
0.439 |
|
| 1998 |
Howard S, He S, Withers SG. Identification of the active site nucleophile in jack bean alpha-mannosidase using 5-fluoro-beta-L-gulosyl fluoride. The Journal of Biological Chemistry. 273: 2067-72. PMID 9442045 DOI: 10.1074/Jbc.273.4.2067 |
0.435 |
|
| 1998 |
Ferro V, Weiler L, Withers SG, Ziltener H. N-Glycosyl phosphonamidates: potential transition-state analogue inhibitors of glycopeptidases Canadian Journal of Chemistry. 76: 313-318. DOI: 10.1139/Cjc-76-3-313 |
0.583 |
|
| 1998 |
Howard S, Withers SG. BromoketoneC-Glycosides, a New Class of β-Glucanase Inactivators† Journal of the American Chemical Society. 120: 10326-10331. DOI: 10.1021/Ja981580R |
0.328 |
|
| 1998 |
Ferro V, Weiler L, Withers SG. Convergent synthesis of a fluorescence-quenched glycopeptide as a potential substrate for peptide: N-glycosidases Carbohydrate Research. 306: 531-538. DOI: 10.1016/S0008-6215(98)00011-1 |
0.59 |
|
| 1997 |
Howard S, Braun C, McCarter J, Moremen KW, Liao YF, Withers SG. Human lysosomal and jack bean α-mannosidases are retaining glycosidases Biochemical and Biophysical Research Communications. 238: 896-898. PMID 9325188 DOI: 10.1006/Bbrc.1997.7148 |
0.367 |
|
| 1997 |
He S, Withers SG. Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. The Journal of Biological Chemistry. 272: 24864-7. PMID 9312086 DOI: 10.1074/Jbc.272.40.24864 |
0.401 |
|
| 1997 |
Mosi R, He S, Uitdehaag J, Dijkstra BW, Withers SG. Trapping and characterization of the reaction intermediate in cyclodextrin glycosyltransferase by use of activated substrates and a mutant enzyme Biochemistry. 36: 9927-9934. PMID 9245426 DOI: 10.1021/Bi970618U |
0.44 |
|
| 1997 |
Mackenzie LF, Davies GJ, Schülein M, Withers SG. Identification of the catalytic nucleophile of endoglucanase I from Fusarium oxysporum by mass spectrometry. Biochemistry. 36: 5893-901. PMID 9153431 DOI: 10.1021/Bi962962H |
0.456 |
|
| 1997 |
Lawson SL, Wakarchuk WW, Withers SG. Positioning the acid/base catalyst in a glycosidase: studies with Bacillus circulans xylanase. Biochemistry. 36: 2257-65. PMID 9047328 DOI: 10.1021/Bi9620215 |
0.36 |
|
| 1997 |
Mackenzie LF, Brooke GS, Cutfield JF, Sullivan PA, Withers SG. Identification of Glu-330 as the catalytic nucleophile of Candida albicans exo-beta-(1,3)-glucanase. The Journal of Biological Chemistry. 272: 3161-7. PMID 9013549 DOI: 10.1074/Jbc.272.6.3161 |
0.45 |
|
| 1997 |
McCarter JD, Burgoyne DL, Miao S, Zhang S, Callahan JW, Withers SG. Identification of Glu-268 as the catalytic nucleophile of human lysosomal beta-galactosidase precursor by mass spectrometry. The Journal of Biological Chemistry. 272: 396-400. PMID 8995274 DOI: 10.1074/Jbc.272.1.396 |
0.421 |
|
| 1997 |
Street IP, Withers SG. Probing the ionization state of substrate alpha-D-glucopyranosyl phosphate bound to glycogen phosphorylase b. The Biochemical Journal. 1017-23. PMID 8948464 DOI: 10.1042/Bj3081017 |
0.42 |
|
| 1997 |
Stirtan WG, Withers SG. Phosphonate and alpha-fluorophosphonate analogue probes of the ionization state of pyridoxal 5'-phosphate (PLP) in glycogen phosphorylase. Biochemistry. 35: 15057-64. PMID 8942672 DOI: 10.1021/Bi9606004 |
0.427 |
|
| 1997 |
Knapp S, Kirk B, Vocadlo D, Withers S. An Allosamizoline/Glucosamine Hybrid NAGase Inhibitor Synlett. 1997: 435-436. DOI: 10.1055/S-1997-6124 |
0.536 |
|
| 1997 |
Harvey TC, Simiand C, Weiler aL, Withers SG. Synthesis of Cyclic Phosphonate Analogs of Ribose and Arabinose Journal of Organic Chemistry. 62: 6722-6725. DOI: 10.1021/Jo9706382 |
0.31 |
|
| 1997 |
McCarter JD, Yeung W, Chow J, Dolphin D, Withers SG. Design and synthesis of 2'-deoxy-2'-fluorodisaccharides as mechanism- based glycosidase inhibitors that exploit aglycon specificity Journal of the American Chemical Society. 119: 5792-5797. DOI: 10.1021/Ja9627454 |
0.453 |
|
| 1997 |
Prade H, Mackenzie LF, Withers SG. Enzymatic synthesis of disaccharides using Agrobacterium sp. β-glucosidase Carbohydrate Research. 305: 371-381. DOI: 10.1016/S0008-6215(97)10040-4 |
0.32 |
|
| 1997 |
Andersen SM, Ebner M, Ekhart CW, Gradnig G, Legler G, Lundt I, Stütz AE, Withers SG, Wrodnigg T. Two isosteric fluorinated derivatives of the powerful glucosidase inhibitors, 1-deoxynojirimycin and 2,5-dideoxy-2,5-imino-d-mannitol: Syntheses and glucosidase-inhibitory activities of 1,2,5-trideoxy-2-fluoro-1,5-imino-d-glucitol and of 1,2,5-trideoxy-1-fluoro-2,5-imino-d-mannitol Carbohydrate Research. 301: 155-166. DOI: 10.1016/S0008-6215(97)00099-2 |
0.329 |
|
| 1997 |
Hoos R, Vasella A, Rupitz K, Withers SG. d-Glyconhydroximolactams strongly inhibit α-glycosidases Carbohydrate Research. 298: 291-298. DOI: 10.1016/S0008-6215(96)00320-5 |
0.344 |
|
| 1996 |
Lai EC, Morris SA, Street IP, Withers SG. Substituted glycals as probes of glycosidase mechanisms. Bioorganic & Medicinal Chemistry. 4: 1929-37. PMID 9007277 DOI: 10.1016/S0968-0896(96)00175-7 |
0.42 |
|
| 1996 |
MacLeod AM, Tull D, Rupitz K, Warren RA, Withers SG. Mechanistic consequences of mutation of active site carboxylates in a retaining beta-1,4-glycanase from Cellulomonas fimi. Biochemistry. 35: 13165-72. PMID 8855954 DOI: 10.1021/Bi9610616 |
0.47 |
|
| 1996 |
Lawson SL, Wakarchuk WW, Withers SG. Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity. Biochemistry. 35: 10110-8. PMID 8756474 DOI: 10.1021/Bi960586V |
0.409 |
|
| 1996 |
McIntosh LP, Hand G, Johnson PE, Joshi MD, Korner M, Plesniak LA, Ziser L, Wakarchuk WW, Withers SG. The pK(a) of the general acid/base carboxyl group of a glycosidase cycles during catalysis: A 13C-NMR study of Bacillus circulans xylanase Biochemistry. 35: 9958-9966. PMID 8756457 DOI: 10.1021/Bi9613234 |
0.42 |
|
| 1996 |
Tull D, Burgoyne DL, Chow DT, Withers SG, Aebersold R. A mass spectrometry-based approach for probing enzyme active sites: Identification of Glu 127 in Cellulomonas fimi exoglycanase as the residue modified by N-bromoacetyl cellobiosylamine Analytical Biochemistry. 234: 119-125. PMID 8714589 DOI: 10.1006/Abio.1996.0063 |
0.349 |
|
| 1996 |
Mitchell EP, Withers SG, Ermert P, Vasella AT, Garman EF, Oikonomakos NG, Johnson LN. Ternary complex crystal structures of glycogen phosphorylase with the transition state analogue nojirimycin tetrazole and phosphate in the T and R states. Biochemistry. 35: 7341-55. PMID 8652510 DOI: 10.1021/Bi960072W |
0.376 |
|
| 1996 |
McCarter JD, Withers SG. Unequivocal identification of Asp-214 as the catalytic nucleophile of Saccharomyces cerevisiae alpha-glucosidase using 5-fluoro glycosyl fluorides. The Journal of Biological Chemistry. 271: 6889-94. PMID 8636115 DOI: 10.1074/Jbc.271.12.6889 |
0.416 |
|
| 1996 |
Damude HG, Ferro V, Withers SG, Warren RA. Substrate specificity of endoglucanase A from Cellulomonas fimi: fundamental differences between endoglucanases and exoglucanases from family 6. The Biochemical Journal. 315: 467-72. PMID 8615816 DOI: 10.1042/Bj3150467 |
0.441 |
|
| 1996 |
Braun C, Lindhorst T, Madsen NB, Withers SG. Identification of Asp 549 as the catalytic nucleophile of glycogen-debranching enzyme via trapping of the glycosyl-enzyme intermediate. Biochemistry. 35: 5458-63. PMID 8611536 DOI: 10.1021/Bi9526488 |
0.682 |
|
| 1996 |
White A, Tull D, Johns K, Withers SG, Rose DR. Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase. Nature Structural Biology. 3: 149-54. PMID 8564541 DOI: 10.1038/Nsb0296-149 |
0.446 |
|
| 1996 |
Brayer GD, Luo Y, Withers SG. The structure of human pancreatic alpha-amylase at 1.8 A resolution and comparisons with related enzymes. Protein Science : a Publication of the Protein Society. 4: 1730-42. PMID 8528071 DOI: 10.1002/Pro.5560040908 |
0.413 |
|
| 1996 |
Namchuk MN, Withers SG. Mechanism of Agrobacterium beta-glucosidase: kinetic analysis of the role of noncovalent enzyme/substrate interactions. Biochemistry. 34: 16194-202. PMID 8519777 DOI: 10.1021/Bi00049A035 |
0.351 |
|
| 1996 |
White A, Tull D, Johns KL, Withers SG, Rose DR. Structural implication of two saccharide conformations in the active site of a β-glycosidase Acta Crystallographica Section a Foundations of Crystallography. 52: C196-C196. DOI: 10.1107/S0108767396091489 |
0.322 |
|
| 1996 |
Knapp S, Vocadlo D, Gao Z, Kirk B, Lou J, Withers SG. NAG-thiazoline, AnN-Acetyl-β-hexosaminidase Inhibitor That Implicates Acetamido Participation Journal of the American Chemical Society. 118: 6804-6805. DOI: 10.1021/Ja960826U |
0.551 |
|
| 1995 |
Zhang S, McCarter JD, Okamura-Oho Y, Yaghi F, Hinek A, Withers SG, Callahan JW. Kinetic mechanism and characterization of human beta-galactosidase precursor secreted by permanently transfected Chinese hamster ovary cells. The Biochemical Journal. 281-8. PMID 7998946 DOI: 10.1042/Bj3040281 |
0.438 |
|
| 1995 |
Lai EC, Withers SG. Stereochemistry and kinetics of the hydration of 2-acetamido-D-glucal by beta-N-acetylhexosaminidases. Biochemistry. 33: 14743-9. PMID 7993902 DOI: 10.1021/Bi00253A012 |
0.442 |
|
| 1995 |
Damude HG, Withers SG, Kilburn DG, Miller RC, Warren RA. Site-directed mutation of the putative catalytic residues of endoglucanase CenA from Cellulomonas fimi. Biochemistry. 34: 2220-4. PMID 7857933 DOI: 10.1021/Bi00007A016 |
0.41 |
|
| 1995 |
Withers SG, Aebersold R. Approaches to labeling and identification of active site residues in glycosidases. Protein Science : a Publication of the Protein Society. 4: 361-72. PMID 7795519 DOI: 10.1002/Pro.5560040302 |
0.374 |
|
| 1995 |
Braun C, Withers SG. An assay for the transferase activity of glycogen debranching enzyme Carbohydrate Research. 271: 113-118. PMID 7648577 DOI: 10.1016/0008-6215(95)00020-T |
0.39 |
|
| 1995 |
Braun C, Brayer GD, Withers SG. Mechanism-based inhibition of yeast alpha-glucosidase and human pancreatic alpha-amylase by a new class of inhibitors. 2-Deoxy-2,2-difluoro-alpha-glycosides. The Journal of Biological Chemistry. 270: 26778-81. PMID 7592915 DOI: 10.1074/Jbc.270.45.26778 |
0.378 |
|
| 1995 |
Ziser L, Setyawati I, Withers SG. Syntheses and testing of substrates and mechanism-based inactivators for xylanases. Carbohydrate Research. 274: 137-53. PMID 7585703 DOI: 10.1016/0008-6215(95)00080-D |
0.317 |
|
| 1995 |
Wang Q, Trimbur D, Graham R, Warren RA, Withers SG. Identification of the acid/base catalyst in Agrobacterium faecalis beta-glucosidase by kinetic analysis of mutants. Biochemistry. 34: 14554-62. PMID 7578061 DOI: 10.1021/Bi00044A034 |
0.453 |
|
| 1995 |
Gebler JC, Trimbur DE, Warren AJ, Aebersold R, Namchuk M, Withers SG. Substrate-induced inactivation of a crippled beta-glucosidase mutant: identification of the labeled amino acid and mutagenic analysis of its role. Biochemistry. 34: 14547-53. PMID 7578060 DOI: 10.1021/Bi00044A033 |
0.437 |
|
| 1995 |
Withers SG. Enzymatic cleavage of glycosides: How does it happen? Pure and Applied Chemistry. 67: 1673-1682. DOI: 10.1351/Pac199567101673 |
0.462 |
|
| 1995 |
Staedtler P, Hoenig S, Frank R, Withers SG, Hengstenberg W. Identification of the active-site nucleophile in 6-phospho-beta-galactosidase from Staphylococcus aureus by labelling with synthetic inhibitors. Febs Journal. 232: 658-663. DOI: 10.1111/J.1432-1033.1995.658Zz.X |
0.419 |
|
| 1995 |
Wang Q, Withers SG. Substrate-Assisted Catalysis In Glycosidases Journal of the American Chemical Society. 117: 10137-10138. DOI: 10.1021/Ja00145A035 |
0.355 |
|
| 1995 |
Withers SG. Probing of glycosidase active sites through labeling, mutagenesis and kinetic studies Progress in Biotechnology. 10: 97-111. DOI: 10.1016/S0921-0423(06)80097-3 |
0.443 |
|
| 1995 |
Knapp S, Dong Y, Rupitz K, Withers SG. β-Glucosidase inhibitors incorporating pyridinium as a glucosyl mimic Bioorganic & Medicinal Chemistry Letters. 5: 763-766. DOI: 10.1016/0960-894X(95)00111-6 |
0.34 |
|
| 1994 |
Tull D, Withers SG. Mechanisms of cellulases and xylanases: a detailed kinetic study of the exo-beta-1,4-glycanase from Cellulomonas fimi. Biochemistry. 33: 6363-70. PMID 8193153 DOI: 10.1021/Bi00186A041 |
0.394 |
|
| 1994 |
Wang Q, Dechert U, Jirik F, Withers SG. Suicide inactivation of human prostatic acid phosphatase and a phosphotyrosine phosphatase Biochemical and Biophysical Research Communications. 200: 577-583. PMID 8166732 DOI: 10.1006/Bbrc.1994.1487 |
0.379 |
|
| 1994 |
McCarter JD, Adam MJ, Hartman NG, Withers SG. In vivo inhibition of beta-glucosidase and beta-mannosidase activity in rats by 2-deoxy-2-fluoro-beta-glycosyl fluorides and recovery of activity in vivo and in vitro. The Biochemical Journal. 343-8. PMID 8042975 DOI: 10.1042/Bj3010343 |
0.309 |
|
| 1994 |
Miao S, Ziser L, Aebersold R, Withers SG. Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry Biochemistry. 33: 7027-7032. PMID 7911679 DOI: 10.1021/Bi00189A002 |
0.37 |
|
| 1994 |
MacLeod AM, Lindhorst T, Withers SG, Warren RA. The acid/base catalyst in the exoglucanase/xylanase from Cellulomonas fimi is glutamic acid 127: evidence from detailed kinetic studies of mutants. Biochemistry. 33: 6371-6. PMID 7910761 DOI: 10.1021/Bi00186A042 |
0.419 |
|
| 1994 |
Wang Q, Graham RW, Trimbur D, Warren RAJ, Withers SG. Changing Enzymic Reaction Mechanisms by Mutagenesis: Conversion of a Retaining Glucosidase to an Inverting Enzyme Journal of the American Chemical Society. 116: 11594-11595. DOI: 10.1021/Ja00104A060 |
0.398 |
|
| 1994 |
Schnabelrauch M, Vasella A, Withers SG. Synthesis and Evaluation as Irreversible Glycosidase Inhibitors of Mono- and Oligo(glycosylthio)benzoquinones Helvetica Chimica Acta. 77: 778-799. DOI: 10.1002/Hlca.19940770319 |
0.336 |
|
| 1993 |
Burk D, Wang Y, Dombroski D, Berghuis AM, Evans SV, Luo Y, Withers SG, Brayer GD. Isolation, crystallization and preliminary diffraction analyses of human pancreatic alpha-amylase. Journal of Molecular Biology. 230: 1084-5. PMID 8478920 DOI: 10.1006/Jmbi.1993.1221 |
0.32 |
|
| 1993 |
Braun C, Meinke A, Ziser L, Withers SG. Simultaneous high-performance liquid chromatographic determination of both the cleavage pattern and the stereochemical outcome of the hydrolysis reactions catalyzed by various glycosidases. Analytical Biochemistry. 212: 259-62. PMID 8368500 DOI: 10.1006/Abio.1993.1320 |
0.364 |
|
| 1993 |
McCarter JD, Adam MJ, Braun C, Namchuk M, Tull D, Withers SG. Syntheses of 2-deoxy-2-fluoro mono- and oligo-saccharide glycosides from glycals and evaluation as glycosidase inhibitors. Carbohydrate Research. 249: 77-90. PMID 8252556 DOI: 10.1016/0008-6215(93)84061-A |
0.363 |
|
| 1993 |
Mitchell EP, Johnson LN, Ermert P, Vasella AT, Withers SG, Oikonomakos NG. Crystallographic studies towards the catalytic mechanism of glycogen phosphorylase Acta Crystallographica Section a Foundations of Crystallography. 49: c87-c87. DOI: 10.1107/S0108767378097512 |
0.327 |
|
| 1993 |
Ermert P, Vasella A, Weber M, Rupitz K, Withers SG. Configurationally selective transition state analogue inhibitors of glycosidases. A study with nojiritetrazoles, a new class of glycosidase inhibitors Carbohydrate Research. 250: 113-128. DOI: 10.1016/0008-6215(93)84160-8 |
0.349 |
|
| 1992 |
Percival MD, Withers SG. 19F NMR investigations of the catalytic mechanism of phosphoglucomutase using fluorinated substrates and inhibitors. Biochemistry. 31: 505-12. PMID 1531026 DOI: 10.1021/bi00117a029 |
0.617 |
|
| 1992 |
Percival MD, Withers SG. Binding energy and catalysis: deoxyfluoro sugars as probes of hydrogen bonding in phosphoglucomutase. Biochemistry. 31: 498-505. PMID 1531025 DOI: 10.1021/bi00117a028 |
0.606 |
|
| 1992 |
McCarter JD, Adam MJ, Withers SG. Binding energy and catalysis. Fluorinated and deoxygenated glycosides as mechanistic probes of Escherichia coli (lacZ) beta-galactosidase. The Biochemical Journal. 721-7. PMID 1417731 DOI: 10.1042/Bj2860721 |
0.383 |
|
| 1992 |
Street IP, Kempton JB, Withers SG. Inactivation of a beta-glucosidase through the accumulation of a stable 2-deoxy-2-fluoro-alpha-D-glucopyranosyl-enzyme intermediate: a detailed investigation. Biochemistry. 31: 9970-8. PMID 1390781 DOI: 10.1021/Bi00156A016 |
0.448 |
|
| 1992 |
Kempton JB, Withers SG. Mechanism of Agrobacterium beta-glucosidase: kinetic studies. Biochemistry. 31: 9961-9. PMID 1390780 DOI: 10.1021/Bi00156A015 |
0.394 |
|
| 1992 |
Withers SG, Rupitz K, Trimbur D, Warren RA. Mechanistic consequences of mutation of the active site nucleophile Glu 358 in Agrobacterium beta-glucosidase. Biochemistry. 31: 9979-85. PMID 1356439 DOI: 10.1021/Bi00156A017 |
0.44 |
|
| 1992 |
Sprang SR, Madsen NB, Withers SG. Multiple phosphate positions in the catalytic site of glycogen phosphorylase: structure of the pyridoxal-5'-pyrophosphate coenzyme-substrate analog. Protein Science : a Publication of the Protein Society. 1: 1100-11. PMID 1304389 DOI: 10.1002/Pro.5560010904 |
0.651 |
|
| 1992 |
Percival MD, Withers SG. Synthesis and testing of sugar phosphofluoridates and cyclic phosphates as inhibitors of phosphoglucomutase The Journal of Organic Chemistry. 57: 811-817. DOI: 10.1021/Jo00029A007 |
0.619 |
|
| 1991 |
Martin JL, Johnson LN, Withers SG. Comparison of the binding of glucose and glucose 1-phosphate derivatives to T-state glycogen phosphorylase b. Biochemistry. 29: 10745-57. PMID 2125493 DOI: 10.1021/Bi00500A005 |
0.365 |
|
| 1991 |
Liu W, Madsen NB, Braun C, Withers SG. Reassessment of the catalytic mechanism of glycogen debranching enzyme. Biochemistry. 30: 1419-24. PMID 1991122 DOI: 10.1021/Bi00219A036 |
0.691 |
|
| 1991 |
Sprang SR, Withers SG, Goldsmith EJ, Fletterick RJ, Madsen NB. Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate. Science (New York, N.Y.). 254: 1367-71. PMID 1962195 DOI: 10.1126/Science.1962195 |
0.646 |
|
| 1991 |
Withers SG, Umezawa K. Cyclophellitol: a naturally occurring mechanism-based inactivator of beta-glucosidases. Biochemical and Biophysical Research Communications. 177: 532-7. PMID 1904221 DOI: 10.1016/0006-291X(91)92016-D |
0.342 |
|
| 1990 |
Withers SG, Rupitz K. Measurement of active-site homology between potato and rabbit muscle alpha-glucan phosphorylases through use of a linear free energy relationship. Biochemistry. 29: 6405-9. PMID 2207081 DOI: 10.1021/Bi00479A010 |
0.432 |
|
| 1990 |
Berven LA, Dolphin D, Withers SG. The base-catalysed anomerization of dinitrophenyl glycosides: Evidence for a novel reaction mechanism Canadian Journal of Chemistry. 68: 1859-1866. DOI: 10.1139/V90-288 |
0.361 |
|
| 1990 |
Bird P, Dolphin DH, Withers SG. The synthesis of protected 5-azido-5-deoxy-D-glucononitriles as precursors of glycosidase inhibitors Canadian Journal of Chemistry. 68: 317-322. DOI: 10.1139/V90-045 |
0.327 |
|
| 1990 |
Withers SG. The enzymic synthesis and N.M.R. characterisation of specifically deoxygenated and fluorinated glycogens Carbohydrate Research. 197: 61-73. DOI: 10.1016/0008-6215(90)84130-M |
0.346 |
|
| 1989 |
Street IP, Rupitz K, Withers SG. Fluorinated and deoxygenated substrates as probes of transition-state structure in glycogen phosphorylase. Biochemistry. 28: 1581-7. PMID 2719921 DOI: 10.1021/Bi00430A024 |
0.388 |
|
| 1989 |
Caron G, Withers SG. Conduritol aziridine: a new mechanism-based glucosidase inactivator. Biochemical and Biophysical Research Communications. 163: 495-9. PMID 2673241 DOI: 10.1016/0006-291X(89)92164-5 |
0.387 |
|
| 1989 |
Withers SG, Percival MD, Street IP. The synthesis and hydrolysis of a series of deoxy- and deoxyfluoro-α-d- "glucopyranosyl" phosphates Carbohydrate Research. 187: 43-66. DOI: 10.1016/0008-6215(89)80055-2 |
0.646 |
|
| 1988 |
Percival MD, Withers SG. Applications of enzymes in the synthesis and hydrolytic study of 2-deoxy-α-D-glucopyranosyl phosphate Canadian Journal of Chemistry. 66: 1970-1972. DOI: 10.1139/V88-317 |
0.669 |
|
| 1988 |
Withers SG, Street IP. Identification of a covalent .alpha.-D-glucopyranosyl enzyme intermediate formed on a .beta.-glucosidase Journal of the American Chemical Society. 110: 8551-8553. DOI: 10.1021/Ja00233A045 |
0.385 |
|
| 1987 |
Day AG, Withers SG. The purification and characterization of a beta-glucosidase from Alcaligenes faecalis. Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. 64: 914-22. PMID 3096349 DOI: 10.1139/O86-122 |
0.34 |
|
| 1987 |
Withers SG, Street IP, Bird P, Dolphin DH. 2-Deoxy-2-fluoroglucosides: A novel class of mechanism-based glucosidase inhibitors Journal of the American Chemical Society. 109: 7530-7531. DOI: 10.1021/Ja00258A047 |
0.301 |
|
| 1986 |
Day AG, Withers SG. A simple kinetic test to distinguish exo-glucosidase and beta-glucosidase activities. Biochemical and Biophysical Research Communications. 133: 628-32. PMID 3936498 DOI: 10.1016/0006-291X(85)90951-9 |
0.387 |
|
| 1986 |
Withers SG, Dombroski D, Berven LA, Kilburn DG, Miller RC, J. Warren RA, Gilkes NR. Direct 1H N.M.R. determination of the stereochemical course of hydrolyses catalysed by glucanase components of the cellulase complex Biochemical and Biophysical Research Communications. 139: 487-494. PMID 3094517 DOI: 10.1016/S0006-291X(86)80017-1 |
0.406 |
|
| 1986 |
Withers SG, Street JP, Rettig SJ. The preferred conformation of 2-fluoro-2-deoxy β-D-mannopyranosyl fluoride. An X-ray crystallographic and 2-dimensional proton nuclear magnetic resonance study Canadian Journal of Chemistry. 64: 232-236. DOI: 10.1139/V86-040 |
0.319 |
|
| 1986 |
Withers SG, MacLennan DJ, Street IP. The synthesis and hydrolysis of a series of deoxyfluoro-d-glucopyranosyl phosphates Carbohydrate Research. 154: 127-144. DOI: 10.1016/S0008-6215(00)90028-4 |
0.35 |
|
| 1985 |
Withers SG, Madsen NB, Sykes BD. 31P NMR relaxation studies of the activation of the coenzyme phosphate of glycogen phosphorylase. The role of motion of the bound phosphate. Biophysical Journal. 48: 1019-26. PMID 3937556 DOI: 10.1016/S0006-3495(85)83864-9 |
0.646 |
|
| 1985 |
Withers SG, Madsen NB, Sykes BD. Relaxation of individual transitions in an AX spectrum. Use of interference terms to separate the dipolar and chemical-shift anisotropy contributions to the relaxation of 31P and 19F nuclei in macromolecules Journal of Magnetic Resonance (1969). 61: 545-549. DOI: 10.1016/0022-2364(85)90195-7 |
0.639 |
|
| 1984 |
Madsen NB, Withers SG. The catalytic mechanism of phosphorylase; novel role of the coenzyme phosphate. Progress in Clinical and Biological Research. 144: 117-26. PMID 6427781 |
0.61 |
|
| 1982 |
Withers SG, Madsen NB, Sprang SR, Fletterick RJ. Catalytic site of glycogen phosphorylase: structural changes during activation and mechanistic implications. Biochemistry. 21: 5372-82. PMID 7171564 DOI: 10.1021/Bi00264A039 |
0.597 |
|
| 1982 |
Withers SG, Madsen NB, Sykes BD. Covalently activated glycogen phosphorylase: a phosphorus-31 nuclear magnetic resonance and ultracentrifugation analysis. Biochemistry. 21: 6716-22. PMID 6818986 DOI: 10.1021/Bi00269A016 |
0.713 |
|
| 1982 |
Sprang SR, Goldsmith EJ, Fletterick RJ, Withers SG, Madsen NB. Catalytic site of glycogen phosphorylase: structure of the T state and specificity for alpha-D-glucose. Biochemistry. 21: 5364-71. PMID 6816272 DOI: 10.1021/Bi00264A038 |
0.592 |
|
| 1982 |
Withers SG, Shechosky S, Madsen NB. Pyridoxal phosphate is not the acid catalyst in the glycogen phosphorylase catalytic mechanism. Biochemical and Biophysical Research Communications. 108: 322-8. PMID 6816229 DOI: 10.1016/0006-291X(82)91869-1 |
0.616 |
|
| 1981 |
Withers SG, Madsen NB, Sykes BD, Takagi M, Shimomura S, Fukui T. Evidence for direct phosphate-phosphate interaction between pyridoxal phosphate and substrate in the glycogen phosphorylase catalytic mechanism. The Journal of Biological Chemistry. 256: 10759-62. PMID 6793586 |
0.678 |
|
| 1981 |
Withers SG, Madsen NB, Sykes BD. Active form of pyridoxal phosphate in glycogen phosphorylase. Phosphorus-31 nuclear magentic resonance investigation. Biochemistry. 20: 1748-56. PMID 6784759 DOI: 10.1021/Bi00510A007 |
0.744 |
|
| 1980 |
Withers SG, Madsen NB. Nucleotide activation of glycogen phosphorylase b occurs only when the nucleotide phosphate is in a dianionic form. Biochemical and Biophysical Research Communications. 97: 513-9. PMID 6781495 DOI: 10.1016/0006-291X(80)90293-4 |
0.609 |
|
| 1980 |
Legler G, Sinnott ML, Withers SG. Catalysis by β-glucosidase A3 of Aspergillus wentii Journal of the Chemical Society, Perkin Transactions 2. 1376-1383. DOI: 10.1039/P29800001376 |
0.606 |
|
| 1980 |
LEGLER G, SINNOTT ML, WITHERS SG. ChemInform Abstract: CATALYSIS BY β-GLUCOSIDASE A3 OF ASPERGILLUS WENTII Chemischer Informationsdienst. 11. DOI: 10.1002/chin.198049325 |
0.482 |
|
| 1979 |
Withers SG, Sykes BD, Madsen NB, Kasvinsky PJ. Identical structural changes induced in glycogen phosphorylase by two nonexclusive allosteric inhibitors. Biochemistry. 18: 5342-8. PMID 518839 DOI: 10.1021/Bi00591A013 |
0.672 |
|
| 1979 |
Withers SG, Yang DS, Madsen NB, Fletterick RJ. A systematic approach to isomorphous replacement using a series of simple charged mercurials. Biochemical and Biophysical Research Communications. 86: 1044-50. PMID 435308 DOI: 10.1016/0006-291X(79)90222-5 |
0.568 |
|
| 1979 |
Loeffler RS, Sinnott ML, Sykes BD, Withers SG. Interaction of the lacZ beta-galactosidase of Escherichia coli with some beta-D-galactopyranoside competitive inhibitors. The Biochemical Journal. 177: 145-52. PMID 106843 DOI: 10.1042/Bj1770145 |
0.64 |
|
| 1978 |
Sinnott ML, Withers SG. The necessity of magnesium cation for acid assistance aglycone departure in catalysis by Escherichia coli (lacZ) beta-galactosidase. The Biochemical Journal. 175: 539-46. PMID 105722 |
0.589 |
|
| 1978 |
Withers SG, Jullien M, Sinnott ML, Viratelle OM, Yon JM. Dependence upon pH of steady-state parameters for the beta-galactosidase-catalysed hydrolyses of beta-D-galactopyranosyl derivatives of different chemical types. European Journal of Biochemistry / Febs. 87: 249-56. PMID 27358 DOI: 10.1111/J.1432-1033.1978.Tb12373.X |
0.545 |
|
| 1975 |
SINNOTT ML, VIRATELLE OM, WITHERS SG. pH- and Magnesium Ion-Dependence of the Hydrolyses of β-d-Galactopyranosyl Pyridinium Salts Catalysed by Escherichia coli β-Galactosidase Biochemical Society Transactions. 3: 1006-1009. DOI: 10.1042/Bst0031006 |
0.514 |
|
| 1974 |
Sinnott ML, Withers SG. The beta-galactosidase-catalysed hydrolyses of beta-d-galactopyranosyl pyridium salts. Rate-limiting generation of an enzyme-bound galactopyranosyl cation in a process dependent only on aglycone acidity. The Biochemical Journal. 143: 751-62. PMID 4462753 DOI: 10.1042/Bj1430751 |
0.583 |
|
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