Area:
DNA Damage, radiotherapy, microbiology, biophysics
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High-probability grants
According to our matching algorithm, David E. Pettijohn is the likely recipient of the following grants.
Years |
Recipients |
Code |
Title / Keywords |
Matching score |
1985 |
Pettijohn, David E |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Packaging of Dna in Chromosomes @ University of Colorado Denver
The long-term objective of the research is to determine the structure of DNA in chromosomes and to define molecular interactions stabilizing this structure. Investigations of bacterial chromosomes as well as those of Drosophila and Human cell lines are proposed. Specific experiments are intended to investigate the torsional tension in the DNA double-helix as it exists in chromosomes and chromatin in vivo. Methods using psoralen probes are employed to make these measurements. Changes in torsional tension during transcription or replication of specific genes will be investigated. It will be determined if the DNA in chromosomes and chromatin in their natural states in vivo is segregated into domains of supercoiling. The role of protein HU and other bacterial proteins in stabilizing negative DNA supercoils and in determining the amount of unrestrained torsional tension will be studied. Methods utilizing the genetics of interspecies cell hybrids will be developed to investigate the roles of specific non-histone chromosomal proteins in chromosome structure.
|
0.982 |
1985 |
Pettijohn, David E |
T32Activity Code Description: To enable institutions to make National Research Service Awards to individuals selected by them for predoctoral and postdoctoral research training in specified shortage areas. |
Predoctoral Training in Human Genetics @ University of Colorado Denver |
0.982 |
1986 — 1990 |
Pettijohn, David E |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Packing of Dna in Chromosomes @ University of Colorado Denver
The general long-term goal is the elucidation of the molecular interactions organizing DNA packaging in chromosomes and the elucidation of interactions organizing the chromosome structure itself. Emphasis in this project is on defining the prokaryotic proteins that package DNA and on determining the topological restraints imposed on the DNA by these proteins. A new approach is to be developed for isolating plasmid DNA molecules having DNA packaged in a native form and for identifying the proteins responsible for this structure. Other experiments using differential decay of birefringence measurements will determine the relative compaction of DNA due to binding histone-like proteins HU, H and HLP1 singly or together. The DNA twist and writhe brought about by these proteins will also be determined. Another emphasis is on elucidating the functions of certain eukaryotic non-histone chromosomal proteins believed to be structural proteins of the nuclear matrix or chromosome scaffold. Monoclonal antibodies specific for these proteins will be micorinjected into living cells to perturb the normal interactions of these proteins and thereby indicate their roles.
|
0.982 |
1991 — 1994 |
Pettijohn, David E |
R01Activity Code Description: To support a discrete, specified, circumscribed project to be performed by the named investigator(s) in an area representing his or her specific interest and competencies. |
Dna Packaging in Chromosomes @ University of Colorado Denver
DESCRIPTION: (Adapted from the Investigator's Abstract) The general long term goal is the determination of the topological structure of DNA in chromosomes and the protein DNA interactions that organize this structure. The research has a fundamental relationship to developing an understanding of gene regulation mediated by protein-DNA interactions and will therefore provide a basis for understanding abnormalities at this level that occur in many diseases. The specific aims are focused on delineating the way that histone like proteins (HLPs) of bacteria and mammals wrap DNA in transient coils and simultaneously change the DNA helical repeat. The experiments investigate the hypothesis that certain HLPs, and especially the HU protein, mediate DNA structural transitions at many sites in the chromosome (such as looping or wrapping) while overwinding the DNA helical repeat to maintain levels of DNA torsional tension required for gene expression. Newly developed methods for obtaining covalent cyclization of small DNA molecules, while they are wrapped on a protein core of HLPs, provides an approach for determining precisely the DNA helical repeat of bound DNA and for determining parameters of DNA bending and wrapping. Recently discovered eukaryotic proteins that seem to wrap DNA in similar, transient, tight coils will be purified and characterized with respect to their possible analogous effects on DNA structure. Finally, a recently developed technique for introducing purified, labelled proteins into living bacterial cells will be exploited to define the distribution of certain HLPs in chromosomes of growing cells and to delineate the sites of transcriptionally active genes in the nucleoid. The latter experiments will test a postulated dynamic structure of the chromosome in living cells.
|
0.982 |