Leonardo M. Damasceno, Ph.D.
Affiliations: | 2007 | Cornell University, Ithaca, NY, United States |
Area:
Microbiology BiologyGoogle:
"Leonardo Damasceno"Parents
Sign in to add mentor| Carl A. Batt | grad student | 2007 | Cornell | |
| (Pichia pastoris as a recombinant protein expression host: Secretion optimization of a single -chain antibody fragment and of a cancer -testis antigen.) | ||||
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Publications
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| Damasceno L, Ritter G, Batt CA. (2017) Process development for production and purification of the Schistosoma mansoni Sm14 antigen. Protein Expression and Purification |
| Casteluber MC, Damasceno LM, da Silveira WB, et al. (2012) Cloning and expression of a functional core streptavidin in Pichia pastoris: strategies to increase yield. Biotechnology Progress. 28: 1419-25 |
| Damasceno LM, Huang CJ, Batt CA. (2012) Protein secretion in Pichia pastoris and advances in protein production. Applied Microbiology and Biotechnology. 93: 31-9 |
| Lowe AJ, Bardliving CL, Huang CJ, et al. (2011) Expression and purification of cGMP grade NY-ESO-1 for clinical trials. Biotechnology Progress. 27: 435-41 |
| Huang CJ, Damasceno LM, Anderson KA, et al. (2011) A proteomic analysis of the Pichia pastoris secretome in methanol-induced cultures. Applied Microbiology and Biotechnology. 90: 235-47 |
| Huang CJ, Anderson KA, Damasceno LM, et al. (2010) Improved secretion of the cancer-testis antigen SSX2 in Pichia pastoris by deletion of its nuclear localization signal. Applied Microbiology and Biotechnology. 86: 243-53 |
| Damasceno LM, Lee F, Ritter G, et al. (2009) High-level expression of a phage display-derived scFv in Pichia pastoris. Methods in Molecular Biology (Clifton, N.J.). 562: 225-36 |
| Damasceno LM, Anderson KA, Ritter G, et al. (2007) Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris. Applied Microbiology and Biotechnology. 74: 381-9 |
| Pla IA, Damasceno LM, Vannelli T, et al. (2006) Evaluation of Mut+ and MutS Pichia pastoris phenotypes for high level extracellular scFv expression under feedback control of the methanol concentration. Biotechnology Progress. 22: 881-8 |
| Damasceno LM, Pla I, Chang HJ, et al. (2004) An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris. Protein Expression and Purification. 37: 18-26 |