Bryan T. Greenhagen, Ph.D.

Affiliations: 
2003 University of Kentucky, Lexington, KY 
Area:
Biochemistry
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"Bryan Greenhagen"
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Joseph Chappell grad student 2003 University of Kentucky
 (Origins of isoprenoid diversity: A study of structure-function relationships in sesquiterpene synthases.)
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Publications

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O'Maille PE, Malone A, Dellas N, et al. (2008) Quantitative exploration of the catalytic landscape separating divergent plant sesquiterpene synthases. Nature Chemical Biology. 4: 617-23
Greenhagen BT, Shi K, Robinson H, et al. (2008) Crystal structure of the pyocyanin biosynthetic protein PhzS. Biochemistry. 47: 5281-9
Takahashi S, Yeo YS, Zhao Y, et al. (2007) Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates. The Journal of Biological Chemistry. 282: 31744-54
Parsons JF, Greenhagen BT, Shi K, et al. (2007) Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa. Biochemistry. 46: 1821-8
Takahashi S, Yeo Y, Greenhagen BT, et al. (2007) Metabolic engineering of sesquiterpene metabolism in yeast. Biotechnology and Bioengineering. 97: 170-81
Greenhagen BT, O'Maille PE, Noel JP, et al. (2006) Identifying and manipulating structural determinates linking catalytic specificities in terpene synthases. Proceedings of the National Academy of Sciences of the United States of America. 103: 9826-31
Wu S, Schoenbeck MA, Greenhagen BT, et al. (2005) Surrogate splicing for functional analysis of sesquiterpene synthase genes. Plant Physiology. 138: 1322-33
Takahashi S, Zhao Y, O'Maille PE, et al. (2005) Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes. The Journal of Biological Chemistry. 280: 3686-96
O'Maille PE, Tsai MD, Greenhagen BT, et al. (2004) Gene library synthesis by structure-based combinatorial protein engineering. Methods in Enzymology. 388: 75-91
Greenhagen BT, Griggs P, Takahashi S, et al. (2003) Probing sesquiterpene hydroxylase activities in a coupled assay with terpene synthases. Archives of Biochemistry and Biophysics. 409: 385-94
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