Lee Raymond Kroos - Publications

Affiliations: 
Michigan State University, East Lansing, MI 

84 high-probability publications. We are testing a new system for linking publications to authors. You can help! If you notice any inaccuracies, please sign in and mark papers as correct or incorrect matches. If you identify any major omissions or other inaccuracies in the publication list, please let us know.

Year Citation  Score
2024 Saha S, Kroos L. Regulation of late-acting operons by three transcription factors and a CRISPR-Cas component during Myxococcus xanthus development. Molecular Microbiology. PMID 38525557 DOI: 10.1111/mmi.15252  0.395
2020 Parrell D, Kroos L. Channels modestly impact compartment-specific ATP levels during Bacillus subtilis sporulation and a rise in the mother cell ATP level is not necessary for Pro-σ cleavage. Molecular Microbiology. PMID 32515031 DOI: 10.1111/Mmi.14560  0.303
2019 Saha S, Patra P, Igoshin O, Kroos L. Systematic analysis of the Myxococcus xanthus developmental gene regulatory network supports posttranslational regulation of FruA by C-signaling. Molecular Microbiology. PMID 30895656 DOI: 10.1111/Mmi.14249  0.446
2018 Hoang Y, Kroos L. Ultrasensitive response of developing to the addition of nutrient medium correlates with the level of MrpC. Journal of Bacteriology. PMID 30181127 DOI: 10.1128/Jb.00456-18  0.361
2017 Halder S, Parrell D, Whitten D, Feig M, Kroos L. Interaction of intramembrane metalloprotease SpoIVFB with substrate Pro-σK. Proceedings of the National Academy of Sciences of the United States of America. 114: E10677-E10686. PMID 29180425 DOI: 10.1073/Pnas.1711467114  0.32
2017 Parrell D, Zhang Y, Olenic S, Kroos L. Bacillus subtilis Intramembrane Protease RasP Activity in Escherichia coli and in Vitro. Journal of Bacteriology. PMID 28674070 DOI: 10.1128/Jb.00381-17  0.363
2017 Rajagopalan R, Kroos L. The dev Operon Regulates the Timing of Sporulation during Myxococcus xanthus Development. Journal of Bacteriology. PMID 28264995 DOI: 10.1128/Jb.00788-16  0.389
2016 Kroos L. Highly Signal-Responsive Gene Regulatory Network Governing Myxococcus Development. Trends in Genetics : Tig. PMID 27916428 DOI: 10.1016/J.Tig.2016.10.006  0.369
2016 Zhang Y, Halder S, Kerr RA, Parrell D, Ruotolo B, Kroos L. Complex Formed between Intramembrane Metalloprotease SpoIVFB and Its Substrate Pro-σK. The Journal of Biological Chemistry. PMID 26953342 DOI: 10.1074/Jbc.M116.715508  0.301
2015 Rajagopalan R, Wielgoss S, Lippert G, Velicer GJ, Kroos L. devI is an evolutionarily young negative regulator of Myxococcus xanthus development. Journal of Bacteriology. 197: 1249-62. PMID 25645563 DOI: 10.1128/Jb.02542-14  0.401
2015 Campbell A, Viswanathan P, Barrett T, Son B, Saha S, Kroos L. Combinatorial regulation of the dev operon by MrpC2 and FruA during Myxococcus xanthus development. Journal of Bacteriology. 197: 240-51. PMID 25349159 DOI: 10.1128/Jb.02310-14  0.534
2014 Robinson M, Son B, Kroos D, Kroos L. Transcription factor MrpC binds to promoter regions of hundreds of developmentally-regulated genes in Myxococcus xanthus. Bmc Genomics. 15: 1123. PMID 25515642 DOI: 10.1186/1471-2164-15-1123  0.541
2014 Rajagopalan R, Kroos L. Nutrient-regulated proteolysis of MrpC halts expression of genes important for commitment to sporulation during Myxococcus xanthus development. Journal of Bacteriology. 196: 2736-47. PMID 24837289 DOI: 10.1128/Jb.01692-14  0.397
2014 Chen B, Himes P, Liu Y, Zhang Y, Lu Z, Liu A, Yan H, Kroos L. Structure of bacterial transcription factor SpoIIID and evidence for a novel mode of DNA binding. Journal of Bacteriology. 196: 2131-42. PMID 24584501 DOI: 10.1128/Jb.01486-13  0.716
2014 Konovalova A, Søgaard-Andersen L, Kroos L. Regulated proteolysis in bacterial development. Fems Microbiology Reviews. 38: 493-522. PMID 24354618 DOI: 10.1111/1574-6976.12050  0.315
2013 Zhou R, Chen K, Xiang X, Gu L, Kroos L. Features of Pro-σK important for cleavage by SpoIVFB, an intramembrane metalloprotease. Journal of Bacteriology. 195: 2793-806. PMID 23585539 DOI: 10.1128/Jb.00229-13  0.335
2011 Son B, Liu Y, Kroos L. Combinatorial regulation by MrpC2 and FruA involves three sites in the fmgE promoter region during Myxococcus xanthus development. Journal of Bacteriology. 193: 2756-66. PMID 21441502 DOI: 10.1128/Jb.00205-11  0.47
2011 Imamura D, Kuwana R, Kroos L, Feig M, Takamatsu H, Watabe K. Substrate specificity of SpoIIGA, a signal-transducing aspartic protease in Bacilli. Journal of Biochemistry. 149: 665-71. PMID 21362630 DOI: 10.1093/Jb/Mvr027  0.314
2011 Lee JS, Son B, Viswanathan P, Luethy PM, Kroos L. Combinatorial regulation of fmgD by MrpC2 and FruA during Myxococcus xanthus development. Journal of Bacteriology. 193: 1681-9. PMID 21257775 DOI: 10.1128/Jb.01541-10  0.502
2010 Kaiser D, Robinson M, Kroos L. Myxobacteria, polarity, and multicellular morphogenesis. Cold Spring Harbor Perspectives in Biology. 2: a000380. PMID 20610548 DOI: 10.1101/Cshperspect.A000380  0.326
2010 Himes P, McBryant SJ, Kroos L. Two regions of Bacillus subtilis transcription factor SpoIIID allow a monomer to bind DNA. Journal of Bacteriology. 192: 1596-606. PMID 20061473 DOI: 10.1128/Jb.01506-09  0.728
2009 Zhou R, Cusumano C, Sui D, Garavito RM, Kroos L. Intramembrane proteolytic cleavage of a membrane-tethered transcription factor by a metalloprotease depends on ATP. Proceedings of the National Academy of Sciences of the United States of America. 106: 16174-9. PMID 19805276 DOI: 10.1073/Pnas.0901455106  0.398
2009 Mittal S, Kroos L. Combinatorial regulation by a novel arrangement of FruA and MrpC2 transcription factors during Myxococcus xanthus development. Journal of Bacteriology. 191: 2753-63. PMID 19201804 DOI: 10.1128/Jb.01818-08  0.68
2009 Harry KH, Zhou R, Kroos L, Melville SB. Sporulation and enterotoxin (CPE) synthesis are controlled by the sporulation-specific sigma factors SigE and SigK in Clostridium perfringens. Journal of Bacteriology. 191: 2728-42. PMID 19201796 DOI: 10.1128/Jb.01839-08  0.436
2009 Mittal S, Kroos L. A combination of unusual transcription factors binds cooperatively to control Myxococcus xanthus developmental gene expression. Proceedings of the National Academy of Sciences of the United States of America. 106: 1965-70. PMID 19179401 DOI: 10.1073/Pnas.0808516106  0.647
2008 Imamura D, Zhou R, Feig M, Kroos L. Evidence that the Bacillus subtilis SpoIIGA protein is a novel type of signal-transducing aspartic protease. The Journal of Biological Chemistry. 283: 15287-99. PMID 18378688 DOI: 10.1074/Jbc.M708962200  0.381
2007 Kroos L. The Bacillus and Myxococcus developmental networks and their transcriptional regulators. Annual Review of Genetics. 41: 13-39. PMID 18076324 DOI: 10.1146/Annurev.Genet.41.110306.130400  0.416
2007 Wang L, Perpich J, Driks A, Kroos L. One perturbation of the mother cell gene regulatory network suppresses the effects of another during sporulation of Bacillus subtilis. Journal of Bacteriology. 189: 8467-73. PMID 17890309 DOI: 10.1128/Jb.01285-07  0.443
2007 Wang L, Perpich J, Driks A, Kroos L. Maintaining the transcription factor SpoIIID level late during sporulation causes spore defects in Bacillus subtilis. Journal of Bacteriology. 189: 7302-9. PMID 17693499 DOI: 10.1128/Jb.00839-07  0.45
2007 Viswanathan P, Ueki T, Inouye S, Kroos L. Combinatorial regulation of genes essential for Myxococcus xanthus development involves a response regulator and a LysR-type regulator. Proceedings of the National Academy of Sciences of the United States of America. 104: 7969-74. PMID 17470804 DOI: 10.1073/Pnas.0701569104  0.504
2007 Viswanathan P, Murphy K, Julien B, Garza AG, Kroos L. Regulation of dev, an operon that includes genes essential for Myxococcus xanthus development and CRISPR-associated genes and repeats. Journal of Bacteriology. 189: 3738-50. PMID 17369305 DOI: 10.1128/Jb.00187-07  0.442
2006 Yoder-Himes DR, Kroos L. Regulation of the Myxococcus xanthus C-signal-dependent Omega4400 promoter by the essential developmental protein FruA. Journal of Bacteriology. 188: 5167-76. PMID 16816188 DOI: 10.1128/Jb.00318-06  0.523
2006 Viswanathan P, Singer M, Kroos L. Role of sigmaD in regulating genes and signals during Myxococcus xanthus development. Journal of Bacteriology. 188: 3246-56. PMID 16621817 DOI: 10.1128/Jb.188.9.3246-3256.2006  0.446
2006 Viswanathan K, Viswanathan P, Kroos L. Mutational analysis of the Myxococcus xanthus Omega4406 promoter region reveals an upstream negative regulatory element that mediates C-signal dependence. Journal of Bacteriology. 188: 515-24. PMID 16385042 DOI: 10.1128/Jb.188.2.515-524.2006  0.459
2005 Zhou R, Kroos L. Serine proteases from two cell types target different components of a complex that governs regulated intramembrane proteolysis of pro-sigmaK during Bacillus subtilis development. Molecular Microbiology. 58: 835-46. PMID 16238631 DOI: 10.1111/J.1365-2958.2005.04870.X  0.376
2005 Krysan DJ, Ting EL, Abeijon C, Kroos L, Fuller RS. Yapsins are a family of aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiae. Eukaryotic Cell. 4: 1364-74. PMID 16087741 DOI: 10.1128/Ec.4.8.1364-1374.2005  0.349
2005 Loconto J, Viswanathan P, Nowak SJ, Gloudemans M, Kroos L. Identification of the omega4406 regulatory region, a developmental promoter of Myxococcus xanthus, and a DNA segment responsible for chromosomal position-dependent inhibition of gene expression. Journal of Bacteriology. 187: 4149-62. PMID 15937177 DOI: 10.1128/Jb.187.12.4149-4162.2005  0.493
2005 Kroos L. Eukaryotic-like signaling and gene regulation in a prokaryote that undergoes multicellular development. Proceedings of the National Academy of Sciences of the United States of America. 102: 2681-2. PMID 15710872 DOI: 10.1073/Pnas.0500157102  0.513
2005 Prince H, Zhou R, Kroos L. Substrate requirements for regulated intramembrane proteolysis of Bacillus subtilis pro-sigmaK. Journal of Bacteriology. 187: 961-71. PMID 15659674 DOI: 10.1128/Jb.187.3.961-971.2005  0.328
2004 Srinivasan D, Kroos L. Mutational analysis of the fruA promoter region demonstrates that C-Box and 5-base-pair elements are important for expression of an essential developmental gene of Myxococcus xanthus. Journal of Bacteriology. 186: 5961-7. PMID 15317804 DOI: 10.1128/Jb.186.17.5961-5967.2004  0.426
2004 Yoder DR, Kroos L. Mutational analysis of the Myxococcus xanthus Omicron4499 promoter region reveals shared and unique properties in comparison with other C-signal-dependent promoters. Journal of Bacteriology. 186: 3766-76. PMID 15175290 DOI: 10.1128/Jb.186.12.3766-3776.2004  0.438
2004 Zhou R, Kroos L. BofA protein inhibits intramembrane proteolysis of pro-sigmaK in an intercompartmental signaling pathway during Bacillus subtilis sporulation. Proceedings of the National Academy of Sciences of the United States of America. 101: 6385-90. PMID 15087499 DOI: 10.1073/Pnas.0307709101  0.367
2004 Yoder DR, Kroos L. Mutational analysis of the Myxococcus xanthus Omega4400 promoter region provides insight into developmental gene regulation by C signaling. Journal of Bacteriology. 186: 661-71. PMID 14729691 DOI: 10.1128/Jb.186.3.661-671.2004  0.457
2003 Viswanathan P, Kroos L. cis Elements necessary for developmental expression of a Myxococcus xanthus gene that depends on C signaling. Journal of Bacteriology. 185: 1405-14. PMID 12562812 DOI: 10.1128/Jb.185.4.1405-1414.2003  0.397
2002 Kroos L, Yu YT, Mills D, Ferguson-Miller S. Forespore signaling is necessary for pro-sigmaK processing during Bacillus subtilis sporulation despite the loss of SpoIVFA upon translational arrest. Journal of Bacteriology. 184: 5393-401. PMID 12218026 DOI: 10.1128/Jb.184.19.5393-5401.2002  0.327
2002 Crater DL, Wade KH, Resnekov O, Ichikawa HT, Kroos L, Brannigan JA, Moran CP. A mutation in GerE that affects cotC promoter activation in Bacillus subtilis. Biochimica Et Biophysica Acta. 1576: 30-8. PMID 12031481 DOI: 10.1016/S0167-4781(02)00294-4  0.602
2002 Hao T, Biran D, Velicer GJ, Kroos L. Identification of the Omega4514 regulatory region, a developmental promoter of Myxococcus xanthus that is transcribed in vitro by the major vegetative RNA polymerase. Journal of Bacteriology. 184: 3348-59. PMID 12029052 DOI: 10.1128/Jb.184.12.3348-3359.2002  0.51
2002 Velicer GJ, Lenski RE, Kroos L. Rescue of social motility lost during evolution of Myxococcus xanthus in an asocial environment. Journal of Bacteriology. 184: 2719-27. PMID 11976301 DOI: 10.1128/Jb.184.10.2719-2727.2002  0.54
2000 Kroos L, Yu YT. Regulation of sigma factor activity during Bacillus subtilis development. Current Opinion in Microbiology. 3: 553-60. PMID 11121773 DOI: 10.1016/S1369-5274(00)00140-5  0.326
2000 Yu YT, Kroos L. Evidence that SpoIVFB is a novel type of membrane metalloprotease governing intercompartmental communication during Bacillus subtilis sporulation. Journal of Bacteriology. 182: 3305-9. PMID 10809718 DOI: 10.1128/Jb.182.11.3305-3309.2000  0.33
2000 Ichikawa H, Kroos L. Combined action of two transcription factors regulates genes encoding spore coat proteins of Bacillus subtilis. The Journal of Biological Chemistry. 275: 13849-55. PMID 10788508 DOI: 10.1074/Jbc.275.18.13849  0.652
2000 Velicer GJ, Kroos L, Lenski RE. Developmental cheating in the social bacterium Myxococcus xanthus. Nature. 404: 598-601. PMID 10766241 DOI: 10.1038/35007066  0.511
1999 Fisseha M, Biran D, Kroos L. Identification of the Omega4499 regulatory region controlling developmental expression of a Myxococcus xanthus cytochrome P-450 system. Journal of Bacteriology. 181: 5467-75. PMID 10464222 DOI: 10.1128/Jb.181.17.5467-5475.1999  0.476
1999 Zhang B, Struffi P, Kroos L. sigmaK can negatively regulate sigE expression by two different mechanisms during sporulation of Bacillus subtilis. Journal of Bacteriology. 181: 4081-8. PMID 10383978 DOI: 10.1128/Jb.181.13.4081-4088.1999  0.444
1999 Kroos L, Zhang B, Ichikawa H, Yu YT. Control of sigma factor activity during Bacillus subtilis sporulation. Molecular Microbiology. 31: 1285-94. PMID 10200951 DOI: 10.1046/J.1365-2958.1999.01214.X  0.585
1999 Ichikawa H, Halberg R, Kroos L. Negative regulation by the Bacillus subtilis GerE protein. The Journal of Biological Chemistry. 274: 8322-7. PMID 10075739 DOI: 10.1074/Jbc.274.12.8322  0.653
1998 Velicer GJ, Kroos L, Lenski RE. Loss of social behaviors by myxococcus xanthus during evolution in an unstructured habitat. Proceedings of the National Academy of Sciences of the United States of America. 95: 12376-80. PMID 9770494 DOI: 10.1073/Pnas.95.21.12376  0.48
1998 Zhang B, Hofmeister A, Kroos L. The prosequence of pro-sigmaK promotes membrane association and inhibits RNA polymerase core binding. Journal of Bacteriology. 180: 2434-41. PMID 9573196 DOI: 10.1128/Jb.180.9.2434-2441.1998  0.343
1998 Brandner JP, Kroos L. Identification of the omega4400 regulatory region, a developmental promoter of Myxococcus xanthus. Journal of Bacteriology. 180: 1995-2004. PMID 9555878 DOI: 10.1128/Jb.180.8.1995-2004.1998  0.544
1997 Zhang B, Kroos L. A feedback loop regulates the switch from one sigma factor to the next in the cascade controlling Bacillus subtilis mother cell gene expression. Journal of Bacteriology. 179: 6138-44. PMID 9324264 DOI: 10.1128/Jb.179.19.6138-6144.1997  0.467
1997 Biran D, Kroos L. In vitro transcription of Myxococcus xanthus genes with RNA polymerase containing sigmaA, the major sigma factor in growing cells. Molecular Microbiology. 25: 463-72. PMID 9302009 DOI: 10.1046/J.1365-2958.1997.4751843.X  0.411
1997 Zhang B, Daniel RA, Errington J, Kroos L. Bacillus subtilis SpoIIID protein binds to two sites in the spoVD promoter and represses transcription by sigmaE RNA polymerase. Journal of Bacteriology. 179: 972-5. PMID 9006059 DOI: 10.1128/Jb.179.3.972-975.1997  0.501
1996 Fisseha M, Gloudemans M, Gill RE, Kroos L. Characterization of the regulatory region of a cell interaction-dependent gene in Myxococcus xanthus. Journal of Bacteriology. 178: 2539-50. PMID 8626320 DOI: 10.1128/Jb.178.9.2539-2550.1996  0.515
1996 Carlson HC, Lu S, Kroos L, Haldenwang WG. Exchange of precursor-specific elements between Pro-sigma E and Pro-sigma K of Bacillus subtilis. Journal of Bacteriology. 178: 546-9. PMID 8550479 DOI: 10.1128/Jb.178.2.546-549.1996  0.306
1995 Halberg R, Oke V, Kroos L. Effects of Bacillus subtilis sporulation regulatory protein SpoIIID on transcription by sigma K RNA polymerase in vivo and in vitro. Journal of Bacteriology. 177: 1888-91. PMID 7896717 DOI: 10.1128/Jb.177.7.1888-1891.1995  0.427
1995 Lu S, Cutting S, Kroos L. Sporulation protein SpoIVFB from Bacillus subtilis enhances processing of the sigma factor precursor Pro-sigma K in the absence of other sporulation gene products. Journal of Bacteriology. 177: 1082-5. PMID 7860587 DOI: 10.1128/Jb.177.4.1082-1085.1995  0.339
1994 Lu S, Kroos L. Overproducing the Bacillus subtilis mother cell sigma factor precursor, Pro-sigma K, uncouples sigma K-dependent gene expression from dependence on intercompartmental communication. Journal of Bacteriology. 176: 3936-43. PMID 8021176 DOI: 10.1128/Jb.176.13.3936-3943.1994  0.357
1994 Halberg R, Kroos L. Sporulation regulatory protein SpoIIID from Bacillus subtilis activates and represses transcription by both mother-cell-specific forms of RNA polymerase. Journal of Molecular Biology. 243: 425-36. PMID 7966271 DOI: 10.1006/Jmbi.1994.1670  0.453
1994 Zhang J, Ichikawa H, Halberg R, Kroos L, Aronson AI. Regulation of the transcription of a cluster of Bacillus subtilis spore coat genes. Journal of Molecular Biology. 240: 405-15. PMID 7519271 DOI: 10.1006/Jmbi.1994.1456  0.592
1992 Zheng L, Halberg R, Roels S, Ichikawa H, Kroos L, Losick R. Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes. Journal of Molecular Biology. 226: 1037-50. PMID 1518043 DOI: 10.1016/0022-2836(92)91051-P  0.623
1992 Halberg R, Kroos L. Fate of the SpoIIID switch protein during Bacillus subtilis sporulation depends on the mother-cell sigma factor, sigma K. Journal of Molecular Biology. 228: 840-9. PMID 1469717 DOI: 10.1016/0022-2836(92)90868-K  0.413
1990 Kroos L, Kuspa A, Kaiser D. Defects in fruiting body development caused by Tn5 lac insertions in Myxococcus xanthus. Journal of Bacteriology. 172: 484-7. PMID 2152913 DOI: 10.1128/Jb.172.1.484-487.1990  0.639
1990 Lu S, Halberg R, Kroos L. Processing of the mother-cell sigma factor, sigma K, may depend on events occurring in the forespore during Bacillus subtilis development. Proceedings of the National Academy of Sciences of the United States of America. 87: 9722-6. PMID 2124700 DOI: 10.1073/Pnas.87.24.9722  0.344
1990 Cutting S, Oke V, Driks A, Losick R, Lu S, Kroos L. A forespore checkpoint for mother cell gene expression during development in B. subtilis. Cell. 62: 239-50. PMID 2115401 DOI: 10.1016/0092-8674(90)90362-I  0.416
1989 Stragier P, Kunkel B, Kroos L, Losick R. Chromosomal rearrangement generating a composite gene for a developmental transcription factor. Science (New York, N.Y.). 243: 507-12. PMID 2536191 DOI: 10.1126/Science.2536191  0.446
1989 Kunkel B, Kroos L, Poth H, Youngman P, Losick R. Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis. Genes & Development. 3: 1735-44. PMID 2514119 DOI: 10.1101/Gad.3.11.1735  0.422
1989 Kroos L, Kunkel B, Losick R. Switch protein alters specificity of RNA polymerase containing a compartment-specific sigma factor. Science (New York, N.Y.). 243: 526-9. PMID 2492118 DOI: 10.1126/Science.2492118  0.435
1988 Kroos L, Hartzell P, Stephens K, Kaiser D. A link between cell movement and gene expression argues that motility is required for cell-cell signaling during fruiting body development. Genes & Development. 2: 1677-85. PMID 3145903 DOI: 10.1101/Gad.2.12A.1677  0.4
1988 Sandman K, Kroos L, Cutting S, Youngman P, Losick R. Identification of the promoter for a spore coat protein gene in Bacillus subtilis and studies on the regulation of its induction at a late stage of sporulation. Journal of Molecular Biology. 200: 461-73. PMID 3135411 DOI: 10.1016/0022-2836(88)90536-0  0.436
1987 Kroos L, Kaiser D. Expression of many developmentally regulated genes in Myxococcus depends on a sequence of cell interactions. Genes & Development. 1: 840-54. PMID 2828174 DOI: 10.1101/Gad.1.8.840  0.407
1986 Kuspa A, Kroos L, Kaiser D. Intercellular signaling is required for developmental gene expression in Myxococcus xanthus. Developmental Biology. 117: 267-76. PMID 3017795 DOI: 10.1016/0012-1606(86)90369-6  0.644
1986 Kroos L, Kuspa A, Kaiser D. A global analysis of developmentally regulated genes in Myxococcus xanthus. Developmental Biology. 117: 252-66. PMID 3017794 DOI: 10.1016/0012-1606(86)90368-4  0.632
1985 Kaiser D, Kroos L, Kuspa A. Cell interactions govern the temporal pattern of Myxococcus development. Cold Spring Harbor Symposia On Quantitative Biology. 50: 823-30. PMID 3007019 DOI: 10.1101/Sqb.1985.050.01.100  0.591
1984 Kroos L, Kaiser D. Construction of Tn5 lac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus. Proceedings of the National Academy of Sciences of the United States of America. 81: 5816-20. PMID 6091110 DOI: 10.1073/Pnas.81.18.5816  0.409
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