Christopher T. Walsh, Ph.D. - Publications

Affiliations: 
Harvard Medical School Harvard University, Cambridge, MA, United States 
Area:
Enzymes
Website:
https://bcmp.med.harvard.edu/faculty/walsh

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Year Citation  Score
2018 Walsh CT, Tu BP, Tang Y. Correction to: Eight Kinetically Stable but Thermodynamically Activated Molecules that Power Cell Metabolism. Chemical Reviews. PMID 29620360 DOI: 10.1021/acs.chemrev.8b00181  1
2017 Walsh CT, Tu BP, Tang Y. Eight Kinetically Stable but Thermodynamically Activated Molecules that Power Cell Metabolism. Chemical Reviews. PMID 29272116 DOI: 10.1021/acs.chemrev.7b00510  1
2017 Walsh CT, Tang Y. Recent Advances in Enzymatic Complexity Generation: Cyclization Reactions. Biochemistry. PMID 29236467 DOI: 10.1021/acs.biochem.7b01161  1
2017 Tran HL, Lexa KW, Julien O, Young TS, Walsh CT, Jacobson MP, Wells JA. SAR and molecular mechanics reveal the importance of ring entropy in the biosynthesis and activity of a natural product. Journal of the American Chemical Society. PMID 28170244 DOI: 10.1021/jacs.6b10792  1
2016 Tang MC, Zou Y, Watanabe K, Walsh CT, Tang Y. Oxidative Cyclization in Natural Product Biosynthesis. Chemical Reviews. PMID 27936626 DOI: 10.1021/acs.chemrev.6b00478  1
2016 Dowling DP, Kung Y, Croft AK, Taghizadeh K, Kelly WL, Walsh CT, Drennan CL. Structural elements of an NRPS cyclization domain and its intermodule docking domain. Proceedings of the National Academy of Sciences of the United States of America. PMID 27791103 DOI: 10.1073/pnas.1608615113  0.88
2015 Medema MH, Kottmann R, Yilmaz P, Cummings M, Biggins JB, Blin K, de Bruijn I, Chooi YH, Claesen J, Coates RC, Cruz-Morales P, Duddela S, Düsterhus S, Edwards DJ, Fewer DP, ... ... Walsh CT, et al. Minimum Information about a Biosynthetic Gene cluster. Nature Chemical Biology. 11: 625-31. PMID 26284661 DOI: 10.1038/nchembio.1890  0.92
2015 Walsh CT. A chemocentric view of the natural product inventory. Nature Chemical Biology. 11: 620-4. PMID 26284660 DOI: 10.1038/nchembio.1894  1
2015 Lowry B, Walsh CT, Khosla C. In Vitro Reconstitution of Metabolic Pathways: Insights into Nature's Chemical Logic. Synlett : Accounts and Rapid Communications in Synthetic Organic Chemistry. 26: 1008-1025. PMID 26207083 DOI: 10.1055/s-0034-1380264  0.52
2015 Walsh CT. Insights into the chemical logic and enzymatic machinery of NRPS assembly lines. Natural Product Reports. PMID 26175103 DOI: 10.1039/c5np00035a  1
2015 Tsodikov OV, Hou C, Walsh CT, Garneau-Tsodikova S. Crystal structure of O-methyltransferase CalO6 from the calicheamicin biosynthetic pathway: a case of challenging structure determination at low resolution. Bmc Structural Biology. 15: 13. PMID 26170207 DOI: 10.1186/s12900-015-0040-6  1
2015 Rojas Murcia N, Lee X, Waridel P, Maspoli A, Imker HJ, Chai T, Walsh CT, Reimmann C. The Pseudomonas aeruginosa antimetabolite L -2-amino-4-methoxy-trans-3-butenoic acid (AMB) is made from glutamate and two alanine residues via a thiotemplate-linked tripeptide precursor. Frontiers in Microbiology. 6: 170. PMID 25814981 DOI: 10.3389/fmicb.2015.00170  1
2015 Li B, Wever WJ, Walsh CT, Bowers AA. Correction: Dithiolopyrrolones: biosynthesis, synthesis, and activity of a unique class of disulfide-containing antibiotics. Natural Product Reports. 32: 348-9. PMID 25598489 DOI: 10.1039/c4np90047b  1
2015 Tsodikov OV, Hou C, Walsh CT, Garneau-Tsodikova S. Crystal structure of O-methyltransferase CalO6 from the calicheamicin biosynthetic pathway: A case of challenging structure determination at low resolution Bmc Structural Biology. 15. DOI: 10.1186/s12900-015-0040-6  1
2015 Lowry B, Walsh CT, Khosla C. In Vitro Reconstitution of Metabolic Pathways: Insights into Nature’s Chemical Logic Synlett. DOI: 10.1055/s-0034-1380264  0.52
2015 Walsh CT. Nature loves nitrogen heterocycles Tetrahedron Letters. 56: 3075-3081. DOI: 10.1016/j.tetlet.2014.11.046  1
2014 Walsh CT. Biological matching of chemical reactivity: pairing indole nucleophilicity with electrophilic isoprenoids. Acs Chemical Biology. 9: 2718-28. PMID 25303280 DOI: 10.1021/cb500695k  1
2014 Setser JW, Heemstra JR, Walsh CT, Drennan CL. Crystallographic evidence of drastic conformational changes in the active site of a flavin-dependent N-hydroxylase. Biochemistry. 53: 6063-77. PMID 25184411 DOI: 10.1021/bi500655q  1
2014 Nakayama A, Okano A, Feng Y, Collins JC, Collins KC, Walsh CT, Boger DL. Enzymatic glycosylation of vancomycin aglycon: completion of a total synthesis of vancomycin and N- and C-terminus substituent effects of the aglycon substrate. Organic Letters. 16: 3572-5. PMID 24954524 DOI: 10.1021/ol501568t  1
2014 Walsh CT. Blurring the lines between ribosomal and nonribosomal peptide scaffolds. Acs Chemical Biology. 9: 1653-61. PMID 24883916 DOI: 10.1021/cb5003587  1
2014 Li B, Wever WJ, Walsh CT, Bowers AA. Dithiolopyrrolones: biosynthesis, synthesis, and activity of a unique class of disulfide-containing antibiotics. Natural Product Reports. 31: 905-23. PMID 24835149 DOI: 10.1039/c3np70106a  1
2014 Khosla C, Herschlag D, Cane DE, Walsh CT. Assembly line polyketide synthases: mechanistic insights and unsolved problems. Biochemistry. 53: 2875-83. PMID 24779441 DOI: 10.1021/bi500290t  1
2014 Lim FY, Ames B, Walsh CT, Keller NP. Co-ordination between BrlA regulation and secretion of the oxidoreductase FmqD directs selective accumulation of fumiquinazoline C to conidial tissues in Aspergillus fumigatus. Cellular Microbiology. 16: 1267-83. PMID 24612080 DOI: 10.1111/cmi.12284  1
2014 Jean C, Chen XL, Nam JO, Tancioni I, Uryu S, Lawson C, Ward KK, Walsh CT, Miller NL, Ghassemian M, Turowski P, Dejana E, Weis S, Cheresh DA, Schlaepfer DD. Inhibition of endothelial FAK activity prevents tumor metastasis by enhancing barrier function. The Journal of Cell Biology. 204: 247-63. PMID 24446483 DOI: 10.1083/jcb.201307067  0.6
2014 Walsh CT, Wencewicz TA. Prospects for new antibiotics: a molecule-centered perspective. The Journal of Antibiotics. 67: 7-22. PMID 23756684 DOI: 10.1038/ja.2013.49  1
2013 Gerken T, Walsh CT. Cloning and sequencing of the chaetocin biosynthetic gene cluster. Chembiochem : a European Journal of Chemical Biology. 14: 2256-8. PMID 24123808 DOI: 10.1002/cbic.201300513  1
2013 Gao X, Jiang W, Jiménez-Osés G, Choi MS, Houk KN, Tang Y, Walsh CT. An iterative, bimodular nonribosomal peptide synthetase that converts anthranilate and tryptophan into tetracyclic asperlicins. Chemistry & Biology. 20: 870-8. PMID 23890005 DOI: 10.1016/j.chembiol.2013.04.019  1
2013 Walsh CT, O'Brien RV, Khosla C. Nonproteinogenic amino acid building blocks for nonribosomal peptide and hybrid polyketide scaffolds. Angewandte Chemie (International Ed. in English). 52: 7098-124. PMID 23729217 DOI: 10.1002/anie.201208344  1
2013 Walsh CT, Haynes SW, Ames BD, Gao X, Tang Y. Short pathways to complexity generation: fungal peptidyl alkaloid multicyclic scaffolds from anthranilate building blocks. Acs Chemical Biology. 8: 1366-82. PMID 23659680 DOI: 10.1021/cb4001684  1
2013 Malcolmson SJ, Young TS, Ruby JG, Skewes-Cox P, Walsh CT. The posttranslational modification cascade to the thiopeptide berninamycin generates linear forms and altered macrocyclic scaffolds. Proceedings of the National Academy of Sciences of the United States of America. 110: 8483-8. PMID 23650400 DOI: 10.1073/pnas.1307111110  1
2013 Jiang W, Cacho RA, Chiou G, Garg NK, Tang Y, Walsh CT. EcdGHK are three tailoring iron oxygenases for amino acid building blocks of the echinocandin scaffold. Journal of the American Chemical Society. 135: 4457-66. PMID 23451921 DOI: 10.1021/ja312572v  1
2013 Haynes SW, Gao X, Tang Y, Walsh CT. Complexity generation in fungal peptidyl alkaloid biosynthesis: a two-enzyme pathway to the hexacyclic MDR export pump inhibitor ardeemin. Acs Chemical Biology. 8: 741-8. PMID 23330675 DOI: 10.1021/cb3006787  1
2013 Parker JB, Walsh CT. Action and timing of BacC and BacD in the late stages of biosynthesis of the dipeptide antibiotic bacilysin. Biochemistry. 52: 889-901. PMID 23317005 DOI: 10.1021/bi3016229  1
2013 Arnison PG, Bibb MJ, Bierbaum G, Bowers AA, Bugni TS, Bulaj G, Camarero JA, Campopiano DJ, Challis GL, Clardy J, Cotter PD, Craik DJ, Dawson M, Dittmann E, Donadio S, ... ... Walsh CT, et al. Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature. Natural Product Reports. 30: 108-60. PMID 23165928 DOI: 10.1039/c2np20085f  1
2013 Walsh CT, Wencewicz TA. Flavoenzymes: versatile catalysts in biosynthetic pathways. Natural Product Reports. 30: 175-200. PMID 23051833 DOI: 10.1039/c2np20069d  1
2012 Young TS, Dorrestein PC, Walsh CT. Codon randomization for rapid exploration of chemical space in thiopeptide antibiotic variants. Chemistry & Biology. 19: 1600-10. PMID 23261603 DOI: 10.1016/j.chembiol.2012.10.013  1
2012 Li B, Forseth RR, Bowers AA, Schroeder FC, Walsh CT. A backup plan for self-protection: S-methylation of holomycin biosynthetic intermediates in Streptomyces clavuligerus. Chembiochem : a European Journal of Chemical Biology. 13: 2521-6. PMID 23097183 DOI: 10.1002/cbic.201200536  1
2012 Haynes SW, Gao X, Tang Y, Walsh CT. Assembly of asperlicin peptidyl alkaloids from anthranilate and tryptophan: a two-enzyme pathway generates heptacyclic scaffold complexity in asperlicin E. Journal of the American Chemical Society. 134: 17444-7. PMID 23030663 DOI: 10.1021/ja308371z  1
2012 Cacho RA, Jiang W, Chooi YH, Walsh CT, Tang Y. Identification and characterization of the echinocandin B biosynthetic gene cluster from Emericella rugulosa NRRL 11440. Journal of the American Chemical Society. 134: 16781-90. PMID 22998630 DOI: 10.1021/ja307220z  1
2012 Wencewicz TA, Walsh CT. Pseudomonas syringae self-protection from tabtoxinine-β-lactam by ligase TblF and acetylase Ttr. Biochemistry. 51: 7712-25. PMID 22994681 DOI: 10.1021/bi3011384  1
2012 Gao X, Haynes SW, Ames BD, Wang P, Vien LP, Walsh CT, Tang Y. Cyclization of fungal nonribosomal peptides by a terminal condensation-like domain. Nature Chemical Biology. 8: 823-30. PMID 22902615 DOI: 10.1038/nchembio.1047  1
2012 Goldman PJ, Ryan KS, Hamill MJ, Howard-Jones AR, Walsh CT, Elliott SJ, Drennan CL. An unusual role for a mobile flavin in StaC-like indolocarbazole biosynthetic enzymes. Chemistry & Biology. 19: 855-65. PMID 22840773 DOI: 10.1016/j.chembiol.2012.05.016  1
2012 Parker JB, Walsh CT. Stereochemical outcome at four stereogenic centers during conversion of prephenate to tetrahydrotyrosine by BacABGF in the bacilysin pathway. Biochemistry. 51: 5622-32. PMID 22765234 DOI: 10.1021/bi3006362  1
2012 Lim ST, Miller NL, Chen XL, Tancioni I, Walsh CT, Lawson C, Uryu S, Weis SM, Cheresh DA, Schlaepfer DD. Nuclear-localized focal adhesion kinase regulates inflammatory VCAM-1 expression. The Journal of Cell Biology. 197: 907-19. PMID 22734001 DOI: 10.1083/jcb.201109067  0.6
2012 Bowers AA, Acker MG, Young TS, Walsh CT. Generation of thiocillin ring size variants by prepeptide gene replacement and in vivo processing by Bacillus cereus. Journal of the American Chemical Society. 134: 10313-6. PMID 22686940 DOI: 10.1021/ja302820x  1
2012 Neumann CS, Jiang W, Heemstra JR, Gontang EA, Kolter R, Walsh CT. Biosynthesis of piperazic acid via N5-hydroxy-ornithine in Kutzneria spp. 744. Chembiochem : a European Journal of Chemical Biology. 13: 972-6. PMID 22522643 DOI: 10.1002/cbic.201200054  1
2012 Parker JB, Walsh CT. Olefin isomerization regiochemistries during tandem action of BacA and BacB on prephenate in bacilysin biosynthesis. Biochemistry. 51: 3241-51. PMID 22483065 DOI: 10.1021/bi300254u  1
2012 Chen XL, Nam JO, Jean C, Lawson C, Walsh CT, Goka E, Lim ST, Tomar A, Tancioni I, Uryu S, Guan JL, Acevedo LM, Weis SM, Cheresh DA, Schlaepfer DD. VEGF-induced vascular permeability is mediated by FAK. Developmental Cell. 22: 146-57. PMID 22264731 DOI: 10.1016/j.devcel.2011.11.002  0.6
2012 Walsh CT, Malcolmson SJ, Young TS. Three ring posttranslational circuses: insertion of oxazoles, thiazoles, and pyridines into protein-derived frameworks. Acs Chemical Biology. 7: 429-42. PMID 22206579 DOI: 10.1021/cb200518n  1
2012 Busche A, Gottstein D, Hein C, Ripin N, Pader I, Tufar P, Eisman EB, Gu L, Walsh CT, Sherman DH, Löhr F, Güntert P, Dötsch V. Characterization of molecular interactions between ACP and halogenase domains in the Curacin A polyketide synthase. Acs Chemical Biology. 7: 378-86. PMID 22103656 DOI: 10.1021/cb200352q  1
2012 Walsh CT, Haynes SW, Ames BD. Aminobenzoates as building blocks for natural product assembly lines. Natural Product Reports. 29: 37-59. PMID 22064917 DOI: 10.1039/c1np00072a  1
2012 Garneau-Tsodikova S, Walsh CT. Editorial: Natural products themed issue Medchemcomm. 3: 852-853. DOI: 10.1039/c2md90031a  1
2011 Crawford JM, Mahlstedt SA, Malcolmson SJ, Clardy J, Walsh CT. Dihydrophenylalanine: a prephenate-derived Photorhabdus luminescens antibiotic and intermediate in dihydrostilbene biosynthesis. Chemistry & Biology. 18: 1102-12. PMID 21944749 DOI: 10.1016/j.chembiol.2011.07.009  1
2011 Ames BD, Haynes SW, Gao X, Evans BS, Kelleher NL, Tang Y, Walsh CT. Complexity generation in fungal peptidyl alkaloid biosynthesis: oxidation of fumiquinazoline A to the heptacyclic hemiaminal fumiquinazoline C by the flavoenzyme Af12070 from Aspergillus fumigatus. Biochemistry. 50: 8756-69. PMID 21899262 DOI: 10.1021/bi201302w  1
2011 Crawford JM, Portmann C, Kontnik R, Walsh CT, Clardy J. NRPS substrate promiscuity diversifies the xenematides. Organic Letters. 13: 5144-7. PMID 21888371 DOI: 10.1021/ol2020237  1
2011 Walsh CT, Zhang W. Chemical logic and enzymatic machinery for biological assembly of peptidyl nucleoside antibiotics. Acs Chemical Biology. 6: 1000-7. PMID 21851099 DOI: 10.1021/cb200284p  1
2011 Wuest WM, Krahn D, Kaiser M, Walsh CT. Enzymatic timing and tailoring of macrolactamization in syringolin biosynthesis. Organic Letters. 13: 4518-21. PMID 21812451 DOI: 10.1021/ol2016687  1
2011 Young TS, Walsh CT. Identification of the thiazolyl peptide GE37468 gene cluster from Streptomyces ATCC 55365 and heterologous expression in Streptomyces lividans. Proceedings of the National Academy of Sciences of the United States of America. 108: 13053-8. PMID 21788474 DOI: 10.1073/pnas.1110435108  1
2011 Zhang W, Ntai I, Kelleher NL, Walsh CT. tRNA-dependent peptide bond formation by the transferase PacB in biosynthesis of the pacidamycin group of pentapeptidyl nucleoside antibiotics. Proceedings of the National Academy of Sciences of the United States of America. 108: 12249-53. PMID 21746899 DOI: 10.1073/pnas.1109539108  1
2011 Jiang W, Heemstra JR, Forseth RR, Neumann CS, Manaviazar S, Schroeder FC, Hale KJ, Walsh CT. Biosynthetic chlorination of the piperazate residue in kutzneride biosynthesis by KthP. Biochemistry. 50: 6063-72. PMID 21648411 DOI: 10.1021/bi200656k  1
2011 Zhang W, Ames BD, Walsh CT. Identification of phenylalanine 3-hydroxylase for meta-tyrosine biosynthesis. Biochemistry. 50: 5401-3. PMID 21615132 DOI: 10.1021/bi200733c  1
2011 Haynes SW, Ames BD, Gao X, Tang Y, Walsh CT. Unraveling terminal C-domain-mediated condensation in fungal biosynthesis of imidazoindolone metabolites. Biochemistry. 50: 5668-79. PMID 21591693 DOI: 10.1021/bi2004922  1
2011 Hollenhorst MA, Ntai I, Badet B, Kelleher NL, Walsh CT. A head-to-head comparison of eneamide and epoxyamide inhibitors of glucosamine-6-phosphate synthase from the dapdiamide biosynthetic pathway. Biochemistry. 50: 3859-61. PMID 21520904 DOI: 10.1021/bi2004735  1
2011 Li B, Walsh CT. Streptomyces clavuligerus HlmI is an intramolecular disulfide-forming dithiol oxidase in holomycin biosynthesis. Biochemistry. 50: 4615-22. PMID 21504228 DOI: 10.1021/bi200321c  1
2011 Balskus EP, Case RJ, Walsh CT. The biosynthesis of cyanobacterial sunscreen scytonemin in intertidal microbial mat communities. Fems Microbiology Ecology. 77: 322-32. PMID 21501195 DOI: 10.1111/j.1574-6941.2011.01113.x  1
2011 Zhang W, Ntai I, Bolla ML, Malcolmson SJ, Kahne D, Kelleher NL, Walsh CT. Nine enzymes are required for assembly of the pacidamycin group of peptidyl nucleoside antibiotics. Journal of the American Chemical Society. 133: 5240-3. PMID 21417270 DOI: 10.1021/ja2011109  1
2011 Gao X, Chooi YH, Ames BD, Wang P, Walsh CT, Tang Y. Fungal indole alkaloid biosynthesis: genetic and biochemical investigation of the tryptoquialanine pathway in Penicillium aethiopicum. Journal of the American Chemical Society. 133: 2729-41. PMID 21299212 DOI: 10.1021/ja1101085  1
2010 Li B, Walsh CT. Identification of the gene cluster for the dithiolopyrrolone antibiotic holomycin in Streptomyces clavuligerus. Proceedings of the National Academy of Sciences of the United States of America. 107: 19731-5. PMID 21041678 DOI: 10.1073/pnas.1014140107  1
2010 Imker HJ, Krahn D, Clerc J, Kaiser M, Walsh CT. N-acylation during glidobactin biosynthesis by the tridomain nonribosomal peptide synthetase module GlbF. Chemistry & Biology. 17: 1077-83. PMID 21035730 DOI: 10.1016/j.chembiol.2010.08.007  1
2010 Zhang W, Heemstra JR, Walsh CT, Imker HJ. Activation of the pacidamycin PacL adenylation domain by MbtH-like proteins. Biochemistry. 49: 9946-7. PMID 20964365 DOI: 10.1021/bi101539b  1
2010 Hollenhorst MA, Bumpus SB, Matthews ML, Bollinger JM, Kelleher NL, Walsh CT. The nonribosomal peptide synthetase enzyme DdaD tethers N(β)-fumaramoyl-l-2,3-diaminopropionate for Fe(II)/α-ketoglutarate-dependent epoxidation by DdaC during dapdiamide antibiotic biosynthesis. Journal of the American Chemical Society. 132: 15773-81. PMID 20945916 DOI: 10.1021/ja1072367  1
2010 Mahlstedt S, Fielding EN, Moore BS, Walsh CT. Prephenate decarboxylases: a new prephenate-utilizing enzyme family that performs nonaromatizing decarboxylation en route to diverse secondary metabolites. Biochemistry. 49: 9021-3. PMID 20863139 DOI: 10.1021/bi101457h  1
2010 Zhang W, Ostash B, Walsh CT. Identification of the biosynthetic gene cluster for the pacidamycin group of peptidyl nucleoside antibiotics. Proceedings of the National Academy of Sciences of the United States of America. 107: 16828-33. PMID 20826445 DOI: 10.1073/pnas.1011557107  1
2010 Balskus EP, Walsh CT. The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science (New York, N.Y.). 329: 1653-6. PMID 20813918 DOI: 10.1126/science.1193637  1
2010 Ames BD, Liu X, Walsh CT. Enzymatic processing of fumiquinazoline F: a tandem oxidative-acylation strategy for the generation of multicyclic scaffolds in fungal indole alkaloid biosynthesis. Biochemistry. 49: 8564-76. PMID 20804163 DOI: 10.1021/bi1012029  1
2010 Bowers AA, Walsh CT, Acker MG. Genetic interception and structural characterization of thiopeptide cyclization precursors from Bacillus cereus. Journal of the American Chemical Society. 132: 12182-4. PMID 20707374 DOI: 10.1021/ja104524q  1
2010 Walsh CT. Catalysis at the intersection of biology, chemistry, and medicine. The Journal of Biological Chemistry. 285: 29681-9. PMID 20595393 DOI: 10.1074/jbc.X110.160309  1
2010 Walsh CT, Acker MG, Bowers AA. Thiazolyl peptide antibiotic biosynthesis: a cascade of post-translational modifications on ribosomal nascent proteins. The Journal of Biological Chemistry. 285: 27525-31. PMID 20522549 DOI: 10.1074/jbc.R110.135970  1
2010 Bowers AA, Acker MG, Koglin A, Walsh CT. Manipulation of thiocillin variants by prepeptide gene replacement: structure, conformation, and activity of heterocycle substitution mutants. Journal of the American Chemical Society. 132: 7519-27. PMID 20455532 DOI: 10.1021/ja102339q  1
2010 Zhang W, Bolla ML, Kahne D, Walsh CT. A three enzyme pathway for 2-amino-3-hydroxycyclopent-2-enone formation and incorporation in natural product biosynthesis. Journal of the American Chemical Society. 132: 6402-11. PMID 20394362 DOI: 10.1021/ja1002845  1
2010 Neumann CS, Walsh CT, Kay RR. A flavin-dependent halogenase catalyzes the chlorination step in the biosynthesis of Dictyostelium differentiation-inducing factor 1. Proceedings of the National Academy of Sciences of the United States of America. 107: 5798-803. PMID 20231486 DOI: 10.1073/pnas.1001681107  1
2010 Ames BD, Walsh CT. Anthranilate-activating modules from fungal nonribosomal peptide assembly lines. Biochemistry. 49: 3351-65. PMID 20225828 DOI: 10.1021/bi100198y  1
2010 Walsh CT, Fischbach MA. Natural products version 2.0: connecting genes to molecules. Journal of the American Chemical Society. 132: 2469-93. PMID 20121095 DOI: 10.1021/ja909118a  1
2010 Mahlstedt SA, Walsh CT. Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis. Biochemistry. 49: 912-23. PMID 20052993 DOI: 10.1021/bi9021186  1
2010 Carretero-Ortega J, Walsh CT, Hernández-García R, Reyes-Cruz G, Brown JH, Vázquez-Prado J. Phosphatidylinositol 3,4,5-triphosphate-dependent Rac exchanger 1 (P-Rex-1), a guanine nucleotide exchange factor for Rac, mediates angiogenic responses to stromal cell-derived factor-1/chemokine stromal cell derived factor-1 (SDF-1/CXCL-12) linked to Rac activation, endothelial cell migration, and in vitro angiogenesis. Molecular Pharmacology. 77: 435-42. PMID 20018810 DOI: 10.1124/mol.109.060400  0.6
2010 Gómez García I, Stevenson CE, Usón I, Freel Meyers CL, Walsh CT, Lawson DM. The crystal structure of the novobiocin biosynthetic enzyme NovP: the first representative structure for the TylF O-methyltransferase superfamily. Journal of Molecular Biology. 395: 390-407. PMID 19857499 DOI: 10.1016/j.jmb.2009.10.045  1
2009 Imker HJ, Walsh CT, Wuest WM. SylC catalyzes ureido-bond formation during biosynthesis of the proteasome inhibitor syringolin A. Journal of the American Chemical Society. 131: 18263-5. PMID 19968303 DOI: 10.1021/ja909170u  1
2009 Acker MG, Bowers AA, Walsh CT. Generation of thiocillin variants by prepeptide gene replacement and in vivo processing by Bacillus cereus. Journal of the American Chemical Society. 131: 17563-5. PMID 19911780 DOI: 10.1021/ja908777t  1
2009 Liu X, Walsh CT. Characterization of cyclo-acetoacetyl-L-tryptophan dimethylallyltransferase in cyclopiazonic acid biosynthesis: substrate promiscuity and site directed mutagenesis studies. Biochemistry. 48: 11032-44. PMID 19877600 DOI: 10.1021/bi901597j  1
2009 Matthews ML, Neumann CS, Miles LA, Grove TL, Booker SJ, Krebs C, Walsh CT, Bollinger JM. Substrate positioning controls the partition between halogenation and hydroxylation in the aliphatic halogenase, SyrB2. Proceedings of the National Academy of Sciences of the United States of America. 106: 17723-8. PMID 19815524 DOI: 10.1073/pnas.0909649106  0.8
2009 Hollenhorst MA, Clardy J, Walsh CT. The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics. Biochemistry. 48: 10467-72. PMID 19807062 DOI: 10.1021/bi9013165  1
2009 Balskus EP, Walsh CT. An enzymatic cyclopentyl[b]indole formation involved in scytonemin biosynthesis. Journal of the American Chemical Society. 131: 14648-9. PMID 19780555 DOI: 10.1021/ja906752u  1
2009 Heemstra JR, Walsh CT, Sattely ES. Enzymatic tailoring of ornithine in the biosynthesis of the Rhizobium cyclic trihydroxamate siderophore vicibactin. Journal of the American Chemical Society. 131: 15317-29. PMID 19778043 DOI: 10.1021/ja9056008  1
2009 Walsh CT, Wright GD. Antimicrobials. Current Opinion in Microbiology. 12: 473-5. PMID 19733112 DOI: 10.1016/j.mib.2009.08.002  1
2009 Frueh DP, Leed A, Arthanari H, Koglin A, Walsh CT, Wagner G. Time-shared HSQC-NOESY for accurate distance constraints measured at high-field in (15)N-(13)C-ILV methyl labeled proteins. Journal of Biomolecular Nmr. 45: 311-8. PMID 19728110 DOI: 10.1007/s10858-009-9372-5  1
2009 Strieker M, Nolan EM, Walsh CT, Marahiel MA. Stereospecific synthesis of threo- and erythro-beta-hydroxyglutamic acid during kutzneride biosynthesis. Journal of the American Chemical Society. 131: 13523-30. PMID 19722489 DOI: 10.1021/ja9054417  1
2009 Fischbach MA, Walsh CT. Antibiotics for emerging pathogens. Science (New York, N.Y.). 325: 1089-93. PMID 19713519 DOI: 10.1126/science.1176667  1
2009 Frueh DP, Arthanari H, Koglin A, Walsh CT, Wagner G. A double TROSY hNCAnH experiment for efficient assignment of large and challenging proteins. Journal of the American Chemical Society. 131: 12880-1. PMID 19702261 DOI: 10.1021/ja9046685  1
2009 Liu X, Walsh CT. Cyclopiazonic acid biosynthesis in Aspergillus sp.: characterization of a reductase-like R* domain in cyclopiazonate synthetase that forms and releases cyclo-acetoacetyl-L-tryptophan. Biochemistry. 48: 8746-57. PMID 19663400 DOI: 10.1021/bi901123r  1
2009 Koglin A, Walsh CT. Structural insights into nonribosomal peptide enzymatic assembly lines. Natural Product Reports. 26: 987-1000. PMID 19636447 DOI: 10.1039/b904543k  1
2009 Walsh CT, Fischbach MA. New ways to squash superbugs. Scientific American. 301: 44-51. PMID 19555023 DOI: 10.1038/scientificamerican0709-44  1
2009 Regni CA, Roush RF, Miller DJ, Nourse A, Walsh CT, Schulman BA. How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic. The Embo Journal. 28: 1953-64. PMID 19494832 DOI: 10.1038/emboj.2009.146  1
2009 Wuest WM, Sattely ES, Walsh CT. Three siderophores from one bacterial enzymatic assembly line. Journal of the American Chemical Society. 131: 5056-7. PMID 19320483 DOI: 10.1021/ja900815w  1
2009 Wong C, Fujimori DG, Walsh CT, Drennan CL. Structural analysis of an open active site conformation of nonheme iron halogenase CytC3. Journal of the American Chemical Society. 131: 4872-9. PMID 19281171 DOI: 10.1021/ja8097355  1
2009 Matthews ML, Krest CM, Barr EW, Vaillancourt FH, Walsh CT, Green MT, Krebs C, Bollinger JM. Substrate-triggered formation and remarkable stability of the C-H bond-cleaving chloroferryl intermediate in the aliphatic halogenase, SyrB2. Biochemistry. 48: 4331-43. PMID 19245217 DOI: 10.1021/bi900109z  1
2009 Strieter ER, Koglin A, Aron ZD, Walsh CT. Cascade reactions during coronafacic acid biosynthesis: elongation, cyclization, and functionalization during Cfa7-catalyzed condensation. Journal of the American Chemical Society. 131: 2113-5. PMID 19199623 DOI: 10.1021/ja8077945  1
2009 Wang Y, Zhou Z, Walsh CT, McMahon AP. Selective translocation of intracellular Smoothened to the primary cilium in response to Hedgehog pathway modulation. Proceedings of the National Academy of Sciences of the United States of America. 106: 2623-8. PMID 19196978 DOI: 10.1073/pnas.0812110106  1
2009 Wieland Brown LC, Acker MG, Clardy J, Walsh CT, Fischbach MA. Thirteen posttranslational modifications convert a 14-residue peptide into the antibiotic thiocillin. Proceedings of the National Academy of Sciences of the United States of America. 106: 2549-53. PMID 19196969 DOI: 10.1073/pnas.0900008106  1
2009 Walsh CT, Fischbach MA. Repurposing libraries of eukaryotic protein kinase inhibitors for antibiotic discovery. Proceedings of the National Academy of Sciences of the United States of America. 106: 1689-90. PMID 19193851 DOI: 10.1073/pnas.0813405106  1
2009 Altmann KH, Buchner J, Kessler H, Diederich F, Kräutler B, Lippard S, Liskamp R, Müller K, Nolan EM, Samori B, Schneider G, Schreiber SL, Schwalbe H, Toniolo C, van Boeckel CA, ... ... Walsh CT, et al. The state of the art of chemical biology. Chembiochem : a European Journal of Chemical Biology. 10: 16-29. PMID 19115274 DOI: 10.1002/cbic.200800758  1
2009 Nolan EM, Walsh CT. How nature morphs peptide scaffolds into antibiotics. Chembiochem : a European Journal of Chemical Biology. 10: 34-53. PMID 19058272 DOI: 10.1002/cbic.200800438  1
2008 Ryan KS, Chakraborty S, Howard-Jones AR, Walsh CT, Ballou DP, Drennan CL. The FAD cofactor of RebC shifts to an IN conformation upon flavin reduction. Biochemistry. 47: 13506-13. PMID 19035832 DOI: 10.1021/bi801229w  1
2008 Gómez García I, Freel Meyers CL, Walsh CT, Lawson DM. Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. 64: 1000-2. PMID 18997325 DOI: 10.1107/S1744309108030145  1
2008 Balskus EP, Walsh CT. Investigating the initial steps in the biosynthesis of cyanobacterial sunscreen scytonemin. Journal of the American Chemical Society. 130: 15260-1. PMID 18954141 DOI: 10.1021/ja807192u  1
2008 Walsh CT, Stupack D, Brown JH. G protein-coupled receptors go extracellular: RhoA integrates the integrins. Molecular Interventions. 8: 165-73. PMID 18829842 DOI: 10.1124/mi.8.4.8  0.6
2008 Neumann CS, Walsh CT. Biosynthesis of (-)-(1S,2R)-allocoronamic acyl thioester by an Fe(II)-dependent halogenase and a cyclopropane-forming flavoprotein. Journal of the American Chemical Society. 130: 14022-3. PMID 18828590 DOI: 10.1021/ja8064667  1
2008 Heemstra JR, Walsh CT. Tandem action of the O2- and FADH2-dependent halogenases KtzQ and KtzR produce 6,7-dichlorotryptophan for kutzneride assembly. Journal of the American Chemical Society. 130: 14024-5. PMID 18828589 DOI: 10.1021/ja806467a  1
2008 Singh GM, Fortin PD, Koglin A, Walsh CT. beta-Hydroxylation of the aspartyl residue in the phytotoxin syringomycin E: characterization of two candidate hydroxylases AspH and SyrP in Pseudomonas syringae. Biochemistry. 47: 11310-20. PMID 18826255 DOI: 10.1021/bi801322z  1
2008 Liu X, Fortin PD, Walsh CT. Andrimid producers encode an acetyl-CoA carboxyltransferase subunit resistant to the action of the antibiotic. Proceedings of the National Academy of Sciences of the United States of America. 105: 13321-6. PMID 18768797 DOI: 10.1073/pnas.0806873105  1
2008 Calderone CT, Bumpus SB, Kelleher NL, Walsh CT, Magarvey NA. A ketoreductase domain in the PksJ protein of the bacillaene assembly line carries out both alpha- and beta-ketone reduction during chain growth. Proceedings of the National Academy of Sciences of the United States of America. 105: 12809-14. PMID 18723688 DOI: 10.1073/pnas.0806305105  1
2008 Sattely ES, Walsh CT. A latent oxazoline electrophile for N-O-C bond formation in pseudomonine biosynthesis. Journal of the American Chemical Society. 130: 12282-4. PMID 18710233 DOI: 10.1021/ja804499r  1
2008 Koglin A, Löhr F, Bernhard F, Rogov VV, Frueh DP, Strieter ER, Mofid MR, Güntert P, Wagner G, Walsh CT, Marahiel MA, Dötsch V. Structural basis for the selectivity of the external thioesterase of the surfactin synthetase. Nature. 454: 907-11. PMID 18704089 DOI: 10.1038/nature07161  1
2008 Frueh DP, Arthanari H, Koglin A, Vosburg DA, Bennett AE, Walsh CT, Wagner G. Dynamic thiolation-thioesterase structure of a non-ribosomal peptide synthetase. Nature. 454: 903-6. PMID 18704088 DOI: 10.1038/nature07162  1
2008 Bumpus SB, Magarvey NA, Kelleher NL, Walsh CT, Calderone CT. Polyunsaturated fatty-acid-like trans-enoyl reductases utilized in polyketide biosynthesis. Journal of the American Chemical Society. 130: 11614-6. PMID 18693732 DOI: 10.1021/ja8040042  1
2008 Nolan EM, Walsh CT. Investigations of the MceIJ-catalyzed posttranslational modification of the microcin E492 C-terminus: linkage of ribosomal and nonribosomal peptides to form "trojan horse" antibiotics. Biochemistry. 47: 9289-99. PMID 18690711 DOI: 10.1021/bi800826j  1
2008 Walsh CT, Radeff-Huang J, Matteo R, Hsiao A, Subramaniam S, Stupack D, Brown JH. Thrombin receptor and RhoA mediate cell proliferation through integrins and cysteine-rich protein 61. Faseb Journal : Official Publication of the Federation of American Societies For Experimental Biology. 22: 4011-21. PMID 18687805 DOI: 10.1096/fj.08-113266  0.6
2008 Sattely ES, Fischbach MA, Walsh CT. Total biosynthesis: in vitro reconstitution of polyketide and nonribosomal peptide pathways. Natural Product Reports. 25: 757-93. PMID 18663394 DOI: 10.1039/b801747f  1
2008 Magarvey NA, Fortin PD, Thomas PM, Kelleher NL, Walsh CT. Gatekeeping versus promiscuity in the early stages of the andrimid biosynthetic assembly line. Acs Chemical Biology. 3: 542-54. PMID 18652473 DOI: 10.1021/cb800085g  1
2008 Zhou Z, Koglin A, Wang Y, McMahon AP, Walsh CT. An eight residue fragment of an acyl carrier protein suffices for post-translational introduction of fluorescent pantetheinyl arms in protein modification in vitro and in vivo. Journal of the American Chemical Society. 130: 9925-30. PMID 18593165 DOI: 10.1021/ja802657n  1
2008 Walsh CT, Fischbach MA. Inhibitors of sterol biosynthesis as Staphylococcus aureus antibiotics. Angewandte Chemie (International Ed. in English). 47: 5700-2. PMID 18576462 DOI: 10.1002/anie.200801801  1
2008 Walsh CT, Nolan EM. Morphing peptide backbones into heterocycles. Proceedings of the National Academy of Sciences of the United States of America. 105: 5655-6. PMID 18398003 DOI: 10.1073/pnas.0802300105  1
2008 Neumann CS, Fujimori DG, Walsh CT. Halogenation strategies in natural product biosynthesis. Chemistry & Biology. 15: 99-109. PMID 18291314 DOI: 10.1016/j.chembiol.2008.01.006  1
2008 Roush RF, Nolan EM, Löhr F, Walsh CT. Maturation of an Escherichia coli ribosomal peptide antibiotic by ATP-consuming N-P bond formation in microcin C7. Journal of the American Chemical Society. 130: 3603-9. PMID 18290647 DOI: 10.1021/ja7101949  1
2008 Fischbach MA, Walsh CT, Clardy J. The evolution of gene collectives: How natural selection drives chemical innovation. Proceedings of the National Academy of Sciences of the United States of America. 105: 4601-8. PMID 18216259 DOI: 10.1073/pnas.0709132105  1
2008 Strieker M, Essen LO, Walsh CT, Marahiel MA. Non-heme hydroxylase engineering for simple enzymatic synthesis of L-threo-hydroxyaspartic acid. Chembiochem : a European Journal of Chemical Biology. 9: 374-6. PMID 18213565 DOI: 10.1002/cbic.200700557  1
2008 Walsh CT, Wei Y, Wientjes MG, Au JL. Quantitative image analysis of intra-tumoral bFGF level as a molecular marker of paclitaxel resistance. Journal of Translational Medicine. 6: 4. PMID 18205918 DOI: 10.1186/1479-5876-6-4  0.6
2008 Ryan KS, Balibar CJ, Turo KE, Walsh CT, Drennan CL. The violacein biosynthetic enzyme VioE shares a fold with lipoprotein transporter proteins. The Journal of Biological Chemistry. 283: 6467-75. PMID 18171675 DOI: 10.1074/jbc.M708573200  1
2008 Balibar CJ, Walsh CT. From thioesters to amides and back: condensation domain reversibility in the biosynthesis of vibriobactin. Chembiochem : a European Journal of Chemical Biology. 9: 42-5. PMID 18022888 DOI: 10.1002/cbic.200700485  1
2008 Walsh CT. The chemical versatility of natural-product assembly lines. Accounts of Chemical Research. 41: 4-10. PMID 17506516 DOI: 10.1021/ar7000414  1
2008 Roush RF, Nolan EM, Löhr F, Walsh CT. Maturation of an Escherichia coli ribosomal peptide antibiotic by ATP-consuming N-P bond formation in microcin C7 (Journal of the American Chemical Society (2008) 130, (3603-3609)) Journal of the American Chemical Society. 130: 6055. DOI: 10.1021/ja802086j  1
2007 Read JA, Walsh CT. The lyngbyatoxin biosynthetic assembly line: chain release by four-electron reduction of a dipeptidyl thioester to the corresponding alcohol. Journal of the American Chemical Society. 129: 15762-3. PMID 18044902 DOI: 10.1021/ja077374d  1
2007 Nolan EM, Fischbach MA, Koglin A, Walsh CT. Biosynthetic tailoring of microcin E492m: post-translational modification affords an antibacterial siderophore-peptide conjugate. Journal of the American Chemical Society. 129: 14336-47. PMID 17973380 DOI: 10.1021/ja074650f  1
2007 Neidig ML, Brown CD, Light KM, Fujimori DG, Nolan EM, Price JC, Barr EW, Bollinger JM, Krebs C, Walsh CT, Solomon EI. CD and MCD of CytC3 and taurine dioxygenase: role of the facial triad in alpha-KG-dependent oxygenases. Journal of the American Chemical Society. 129: 14224-31. PMID 17967013 DOI: 10.1021/ja074557r  1
2007 Fujimori DG, Hrvatin S, Neumann CS, Strieker M, Marahiel MA, Walsh CT. Cloning and characterization of the biosynthetic gene cluster for kutznerides. Proceedings of the National Academy of Sciences of the United States of America. 104: 16498-503. PMID 17940045 DOI: 10.1073/pnas.0708242104  1
2007 Fujimori DG, Barr EW, Matthews ML, Koch GM, Yonce JR, Walsh CT, Bollinger JM, Krebs C, Riggs-Gelasco PJ. Spectroscopic evidence for a high-spin Br-Fe(IV)-oxo intermediate in the alpha-ketoglutarate-dependent halogenase CytC3 from Streptomyces. Journal of the American Chemical Society. 129: 13408-9. PMID 17939667 DOI: 10.1021/ja076454e  1
2007 Fujimori DG, Walsh CT. What's new in enzymatic halogenations. Current Opinion in Chemical Biology. 11: 553-60. PMID 17881282 DOI: 10.1016/j.cbpa.2007.08.002  1
2007 Ryan KS, Howard-Jones AR, Hamill MJ, Elliott SJ, Walsh CT, Drennan CL. Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC. Proceedings of the National Academy of Sciences of the United States of America. 104: 15311-6. PMID 17873060 DOI: 10.1073/pnas.0707190104  1
2007 Howard-Jones AR, Walsh CT. Nonenzymatic oxidative steps accompanying action of the cytochrome P450 enzymes StaP and RebP in the biosynthesis of staurosporine and rebeccamycin. Journal of the American Chemical Society. 129: 11016-7. PMID 17705392 DOI: 10.1021/ja0743801  1
2007 Balibar CJ, Howard-Jones AR, Walsh CT. Terrequinone A biosynthesis through L-tryptophan oxidation, dimerization and bisprenylation. Nature Chemical Biology. 3: 584-92. PMID 17704773 DOI: 10.1038/nchembio.2007.20  1
2007 Leimkuhler C, Fridman M, Lupoli T, Walker S, Walsh CT, Kahne D. Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A. Journal of the American Chemical Society. 129: 10546-50. PMID 17685523 DOI: 10.1021/ja072909o  1
2007 Howard-Jones AR, Kruger RG, Lu W, Tao J, Leimkuhler C, Kahne D, Walsh CT. Kinetic analysis of teicoplanin glycosyltransferases and acyltransferase reveal ordered tailoring of aglycone scaffold to reconstitute mature teicoplanin. Journal of the American Chemical Society. 129: 10082-3. PMID 17661468 DOI: 10.1021/ja0735857  1
2007 Calderone CT, Iwig DF, Dorrestein PC, Kelleher NL, Walsh CT. Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1. Chemistry & Biology. 14: 835-46. PMID 17656320 DOI: 10.1016/j.chembiol.2007.06.008  1
2007 Fortin PD, Walsh CT, Magarvey NA. A transglutaminase homologue as a condensation catalyst in antibiotic assembly lines. Nature. 448: 824-7. PMID 17653193 DOI: 10.1038/nature06068  1
2007 Fischbach MA, Lai JR, Roche ED, Walsh CT, Liu DR. Directed evolution can rapidly improve the activity of chimeric assembly-line enzymes. Proceedings of the National Academy of Sciences of the United States of America. 104: 11951-6. PMID 17620609 DOI: 10.1073/pnas.0705348104  1
2007 Zhou Z, Lai JR, Walsh CT. Directed evolution of aryl carrier proteins in the enterobactin synthetase. Proceedings of the National Academy of Sciences of the United States of America. 104: 11621-6. PMID 17606920 DOI: 10.1073/pnas.0705122104  1
2007 Balibar CJ, Garneau-Tsodikova S, Walsh CT. Covalent CouN7 enzyme intermediate for acyl group shuttling in aminocoumarin biosynthesis. Chemistry & Biology. 14: 679-90. PMID 17584615 DOI: 10.1016/j.chembiol.2007.05.007  1
2007 Fridman M, Balibar CJ, Lupoli T, Kahne D, Walsh CT, Garneau-Tsodikova S. Chemoenzymatic formation of novel aminocoumarin antibiotics by the enzymes CouN1 and CouN7. Biochemistry. 46: 8462-71. PMID 17580964 DOI: 10.1021/bi700433v  1
2007 Krebs C, Galonić Fujimori D, Walsh CT, Bollinger JM. Non-heme Fe(IV)-oxo intermediates. Accounts of Chemical Research. 40: 484-92. PMID 17542550 DOI: 10.1021/ar700066p  0.8
2007 Strieter ER, Vaillancourt FH, Walsh CT. CmaE: a transferase shuttling aminoacyl groups between carrier protein domains in the coronamic acid biosynthetic pathway. Biochemistry. 46: 7549-57. PMID 17530782 DOI: 10.1021/bi700243h  1
2007 Pacholec M, Sello JK, Walsh CT, Thomas MG. Formation of an aminoacyl-S-enzyme intermediate is a key step in the biosynthesis of chloramphenicol. Organic & Biomolecular Chemistry. 5: 1692-4. PMID 17520135 DOI: 10.1039/b703356g  1
2007 Walsh CT. Revealing coupling patterns in isoprenoid alkylation biocatalysis. Acs Chemical Biology. 2: 296-8. PMID 17518430 DOI: 10.1021/cb700094s  1
2007 Hansen DB, Bumpus SB, Aron ZD, Kelleher NL, Walsh CT. The loading module of mycosubtilin: an adenylation domain with fatty acid selectivity. Journal of the American Chemical Society. 129: 6366-7. PMID 17472382 DOI: 10.1021/ja070890j  1
2007 Zhou Z, Cironi P, Lin AJ, Xu Y, Hrvatin S, Golan DE, Silver PA, Walsh CT, Yin J. Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases. Acs Chemical Biology. 2: 337-46. PMID 17465518 DOI: 10.1021/cb700054k  1
2007 Yin J, Straight PD, Hrvatin S, Dorrestein PC, Bumpus SB, Jao C, Kelleher NL, Kolter R, Walsh CT. Genome-wide high-throughput mining of natural-product biosynthetic gene clusters by phage display. Chemistry & Biology. 14: 303-12. PMID 17379145 DOI: 10.1016/j.chembiol.2007.01.006  1
2007 Taga ME, Larsen NA, Howard-Jones AR, Walsh CT, Walker GC. BluB cannibalizes flavin to form the lower ligand of vitamin B12. Nature. 446: 449-53. PMID 17377583 DOI: 10.1038/nature05611  1
2007 Aron ZD, Fortin PD, Calderone CT, Walsh CT. FenF: servicing the Mycosubtilin synthetase assembly line in trans. Chembiochem : a European Journal of Chemical Biology. 8: 613-6. PMID 17330903 DOI: 10.1002/cbic.200600575  1
2007 Stevenson CE, Freel Meyers CL, Walsh CT, Lawson DM. Crystallization and preliminary X-ray analysis of the O-methyltransferase NovP from the novobiocin-biosynthetic cluster of Streptomyces spheroides. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. 63: 236-8. PMID 17329822 DOI: 10.1107/S1744309107008287  1
2007 Yeh E, Blasiak LC, Koglin A, Drennan CL, Walsh CT. Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases. Biochemistry. 46: 1284-92. PMID 17260957 DOI: 10.1021/bi0621213  1
2007 Singh GM, Vaillancourt FH, Yin J, Walsh CT. Characterization of SyrC, an aminoacyltransferase shuttling threonyl and chlorothreonyl residues in the syringomycin biosynthetic assembly line. Chemistry & Biology. 14: 31-40. PMID 17254950 DOI: 10.1016/j.chembiol.2006.11.005  1
2007 Butcher RA, Schroeder FC, Fischbach MA, Straight PD, Kolter R, Walsh CT, Clardy J. The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America. 104: 1506-9. PMID 17234808 DOI: 10.1073/pnas.0610503104  1
2007 Galonić DP, Barr EW, Walsh CT, Bollinger JM, Krebs C. Two interconverting Fe(IV) intermediates in aliphatic chlorination by the halogenase CytC3. Nature Chemical Biology. 3: 113-6. PMID 17220900 DOI: 10.1038/nchembio856  0.8
2007 Kelly WL, Boyne MT, Yeh E, Vosburg DA, Galoni? DP, Kelleher NL, Walsh CT. Characterization of the aminocarboxycyclopropane-forming enzyme CmaC. Biochemistry. 46: 359-68. PMID 17209546 DOI: 10.1021/bi061930j  1
2007 Straight PD, Fischbach MA, Walsh CT, Rudner DZ, Kolter R. A singular enzymatic megacomplex from Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America. 104: 305-10. PMID 17190806 DOI: 10.1073/pnas.0609073103  1
2007 Radeff-Huang J, Seasholtz TM, Chang JW, Smith JM, Walsh CT, Brown JH. Tumor necrosis factor-alpha-stimulated cell proliferation is mediated through sphingosine kinase-dependent Akt activation and cyclin D expression. The Journal of Biological Chemistry. 282: 863-70. PMID 17114809 DOI: 10.1074/jbc.M601698200  0.6
2006 Yin J, Lin AJ, Golan DE, Walsh CT. Site-specific protein labeling by Sfp phosphopantetheinyl transferase. Nature Protocols. 1: 280-5. PMID 17406245 DOI: 10.1038/nprot.2006.43  1
2006 Balibar CJ, Walsh CT. In vitro biosynthesis of violacein from L-tryptophan by the enzymes VioA-E from Chromobacterium violaceum. Biochemistry. 45: 15444-57. PMID 17176066 DOI: 10.1021/bi061998z  1
2006 Luo M, Lin H, Fischbach MA, Liu DR, Walsh CT, Groves JT. Enzymatic tailoring of enterobactin alters membrane partitioning and iron acquisition. Acs Chemical Biology. 1: 29-32. PMID 17163637 DOI: 10.1021/cb0500034  1
2006 Clardy J, Fischbach MA, Walsh CT. New antibiotics from bacterial natural products. Nature Biotechnology. 24: 1541-50. PMID 17160060 DOI: 10.1038/nbt1266  1
2006 Balibar CJ, Walsh CT. GliP, a multimodular nonribosomal peptide synthetase in Aspergillus fumigatus, makes the diketopiperazine scaffold of gliotoxin. Biochemistry. 45: 15029-38. PMID 17154540 DOI: 10.1021/bi061845b  1
2006 Lai JR, Koglin A, Walsh CT. Carrier protein structure and recognition in polyketide and nonribosomal peptide biosynthesis. Biochemistry. 45: 14869-79. PMID 17154525 DOI: 10.1021/bi061979p  1
2006 Ueki M, Galoni? DP, Vaillancourt FH, Garneau-Tsodikova S, Yeh E, Vosburg DA, Schroeder FC, Osada H, Walsh CT. Enzymatic generation of the antimetabolite gamma,gamma-dichloroaminobutyrate by NRPS and mononuclear iron halogenase action in a streptomycete. Chemistry & Biology. 13: 1183-91. PMID 17114000 DOI: 10.1016/j.chembiol.2006.09.012  1
2006 Fischbach MA, Walsh CT. Biochemistry. Directing biosynthesis. Science (New York, N.Y.). 314: 603-5. PMID 17068249 DOI: 10.1126/science.1132692  1
2006 Fischbach MA, Lin H, Zhou L, Yu Y, Abergel RJ, Liu DR, Raymond KN, Wanner BL, Strong RK, Walsh CT, Aderem A, Smith KD. The pathogen-associated iroA gene cluster mediates bacterial evasion of lipocalin 2. Proceedings of the National Academy of Sciences of the United States of America. 103: 16502-7. PMID 17060628 DOI: 10.1073/pnas.0604636103  1
2006 Dorrestein PC, Bumpus SB, Calderone CT, Garneau-Tsodikova S, Aron ZD, Straight PD, Kolter R, Walsh CT, Kelleher NL. Facile detection of acyl and peptidyl intermediates on thiotemplate carrier domains via phosphopantetheinyl elimination reactions during tandem mass spectrometry. Biochemistry. 45: 12756-66. PMID 17042494 DOI: 10.1021/bi061169d  1
2006 Garneau-Tsodikova S, Dorrestein PC, Kelleher NL, Walsh CT. Protein assembly line components in prodigiosin biosynthesis: characterization of PigA,G,H,I,J. Journal of the American Chemical Society. 128: 12600-1. PMID 17002325 DOI: 10.1021/ja063611l  1
2006 Howard-Jones AR, Walsh CT. Staurosporine and rebeccamycin aglycones are assembled by the oxidative action of StaP, StaC, and RebC on chromopyrrolic acid. Journal of the American Chemical Society. 128: 12289-98. PMID 16967980 DOI: 10.1021/ja063898m  0.6
2006 Zhou Z, Lai JR, Walsh CT. Interdomain communication between the thiolation and thioesterase domains of EntF explored by combinatorial mutagenesis and selection. Chemistry & Biology. 13: 869-79. PMID 16931336 DOI: 10.1016/j.chembiol.2006.06.011  1
2006 Lai JR, Fischbach MA, Liu DR, Walsh CT. Localized protein interaction surfaces on the EntB carrier protein revealed by combinatorial mutagenesis and selection. Journal of the American Chemical Society. 128: 11002-3. PMID 16925399 DOI: 10.1021/ja063238h  1
2006 Larsen NA, Lin H, Wei R, Fischbach MA, Walsh CT. Structural characterization of enterobactin hydrolase IroE. Biochemistry. 45: 10184-90. PMID 16922493 DOI: 10.1021/bi060950i  1
2006 Magarvey NA, Ehling-Schulz M, Walsh CT. Characterization of the cereulide NRPS alpha-hydroxy acid specifying modules: activation of alpha-keto acids and chiral reduction on the assembly line. Journal of the American Chemical Society. 128: 10698-9. PMID 16910662 DOI: 10.1021/ja0640187  1
2006 Fischbach MA, Walsh CT. Assembly-line enzymology for polyketide and nonribosomal Peptide antibiotics: logic, machinery, and mechanisms. Chemical Reviews. 106: 3468-96. PMID 16895337 DOI: 10.1021/cr0503097  1
2006 Vaillancourt FH, Yeh E, Vosburg DA, Garneau-Tsodikova S, Walsh CT. Nature's inventory of halogenation catalysts: oxidative strategies predominate. Chemical Reviews. 106: 3364-78. PMID 16895332 DOI: 10.1021/cr050313i  1
2006 Walsh CT, Garneau-Tsodikova S, Howard-Jones AR. Biological formation of pyrroles: nature's logic and enzymatic machinery. Natural Product Reports. 23: 517-31. PMID 16874387 DOI: 10.1039/b605245m  1
2006 Lin H, Fischbach MA, Gatto GJ, Liu DR, Walsh CT. Bromoenterobactins as potent inhibitors of a pathogen-associated, siderophore-modifying C-glycosyltransferase. Journal of the American Chemical Society. 128: 9324-5. PMID 16848455 DOI: 10.1021/ja063236x  1
2006 Garneau-Tsodikova S, Stapon A, Kahne D, Walsh CT. Installation of the pyrrolyl-2-carboxyl pharmacophore by CouN1 and CouN7 in the late biosynthetic steps of the aminocoumarin antibiotics clorobiocin and coumermycin A1. Biochemistry. 45: 8568-78. PMID 16834331 DOI: 10.1021/bi060784e  1
2006 Hicks LM, Balibar CJ, Walsh CT, Kelleher NL, Hillson NJ. Probing intra- versus interchain kinetic preferences of L-Thr acylation on dimeric VibF with mass spectrometry. Biophysical Journal. 91: 2609-19. PMID 16815901 DOI: 10.1529/biophysj.106.084848  1
2006 Yeh E, Cole LJ, Barr EW, Bollinger JM, Ballou DP, Walsh CT. Flavin redox chemistry precedes substrate chlorination during the reaction of the flavin-dependent halogenase RebH. Biochemistry. 45: 7904-12. PMID 16784243 DOI: 10.1021/bi060607d  1
2006 Calderone CT, Kowtoniuk WE, Kelleher NL, Walsh CT, Dorrestein PC. Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America. 103: 8977-82. PMID 16757561 DOI: 10.1073/pnas.0603148103  1
2006 Frueh DP, Sun ZY, Vosburg DA, Walsh CT, Hoch JC, Wagner G. Non-uniformly sampled double-TROSY hNcaNH experiments for NMR sequential assignments of large proteins. Journal of the American Chemical Society. 128: 5757-63. PMID 16637644 DOI: 10.1021/ja0584222  1
2006 Lai JR, Fischbach MA, Liu DR, Walsh CT. A protein interaction surface in nonribosomal peptide synthesis mapped by combinatorial mutagenesis and selection. Proceedings of the National Academy of Sciences of the United States of America. 103: 5314-9. PMID 16567620 DOI: 10.1073/pnas.0601038103  1
2006 Galonić DP, Vaillancourt FH, Walsh CT. Halogenation of unactivated carbon centers in natural product biosynthesis: trichlorination of leucine during barbamide biosynthesis. Journal of the American Chemical Society. 128: 3900-1. PMID 16551084 DOI: 10.1021/ja060151n  1
2006 Blasiak LC, Vaillancourt FH, Walsh CT, Drennan CL. Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis. Nature. 440: 368-71. PMID 16541079 DOI: 10.1038/nature04544  1
2006 Gatto GJ, Boyne MT, Kelleher NL, Walsh CT. Biosynthesis of pipecolic acid by RapL, a lysine cyclodeaminase encoded in the rapamycin gene cluster. Journal of the American Chemical Society. 128: 3838-47. PMID 16536560 DOI: 10.1021/ja0587603  1
2006 Vaillancourt FH, Vosburg DA, Walsh CT. Dichlorination and bromination of a threonyl-S-carrier protein by the non-heme Fe(II) halogenase SyrB2. Chembiochem : a European Journal of Chemical Biology. 7: 748-52. PMID 16528784 DOI: 10.1002/cbic.200500480  1
2006 Tello M, Jakimowicz P, Errey JC, Freel Meyers CL, Walsh CT, Buttner MJ, Lawson DM, Field RA. Characterisation of Streptomyces spheroides NovW and revision of its functional assignment to a dTDP-6-deoxy-D-xylo-4-hexulose 3-epimerase. Chemical Communications (Cambridge, England). 1079-81. PMID 16514445 DOI: 10.1039/b515763c  1
2006 Frueh DP, Vosburg DA, Walsh CT, Wagner G. Determination of all nOes in 1H-13C-Me-ILV-U-2H-15N proteins with two time-shared experiments. Journal of Biomolecular Nmr. 34: 31-40. PMID 16505962 DOI: 10.1007/s10858-005-5338-4  1
2006 Fischbach MA, Lin H, Liu DR, Walsh CT. How pathogenic bacteria evade mammalian sabotage in the battle for iron. Nature Chemical Biology. 2: 132-8. PMID 16485005 DOI: 10.1038/nchembio771  1
2006 Dorrestein PC, Blackhall J, Straight PD, Fischbach MA, Garneau-Tsodikova S, Edwards DJ, McLaughlin S, Lin M, Gerwick WH, Kolter R, Walsh CT, Kelleher NL. Activity screening of carrier domains within nonribosomal peptide synthetases using complex substrate mixtures and large molecule mass spectrometry. Biochemistry. 45: 1537-46. PMID 16460000 DOI: 10.1021/bi052333k  1
2006 Jakimowicz P, Tello M, Meyers CL, Walsh CT, Buttner MJ, Field RA, Lawson DM. The 1.6-A resolution crystal structure of NovW: a 4-keto-6-deoxy sugar epimerase from the novobiocin biosynthetic gene cluster of Streptomyces spheroides. Proteins. 63: 261-5. PMID 16411240 DOI: 10.1002/prot.20818  1
2005 Howard-Jones AR, Walsh CT. Enzymatic generation of the chromopyrrolic acid scaffold of rebeccamycin by the tandem action of RebO and RebD. Biochemistry. 44: 15652-63. PMID 16313168 DOI: 10.1021/bi051706e  0.6
2005 Balibar CJ, Vaillancourt FH, Walsh CT. Generation of D amino acid residues in assembly of arthrofactin by dual condensation/epimerization domains. Chemistry & Biology. 12: 1189-200. PMID 16298298 DOI: 10.1016/j.chembiol.2005.08.010  1
2005 Pacholec M, Tao J, Walsh CT. CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis. Biochemistry. 44: 14969-76. PMID 16274243 DOI: 10.1021/bi051599o  1
2005 Walsh CT, Garneau-Tsodikova S, Gatto GJ. Protein posttranslational modifications: the chemistry of proteome diversifications. Angewandte Chemie (International Ed. in English). 44: 7342-72. PMID 16267872 DOI: 10.1002/anie.200501023  1
2005 Aron ZD, Dorrestein PC, Blackhall JR, Kelleher NL, Walsh CT. Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster. Journal of the American Chemical Society. 127: 14986-7. PMID 16248612 DOI: 10.1021/ja055247g  1
2005 McLoughlin SM, Mazur MT, Miller LM, Yin J, Liu F, Walsh CT, Kelleher NL. Chemoenzymatic approaches for streamlined detection of active site modifications on thiotemplate assembly lines using mass spectrometry. Biochemistry. 44: 14159-69. PMID 16245932 DOI: 10.1021/bi051202g  1
2005 Yin J, Straight PD, McLoughlin SM, Zhou Z, Lin AJ, Golan DE, Kelleher NL, Kolter R, Walsh CT. Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase. Proceedings of the National Academy of Sciences of the United States of America. 102: 15815-20. PMID 16236721 DOI: 10.1073/pnas.0507705102  1
2005 Yuan Y, Chung HS, Leimkuhler C, Walsh CT, Kahne D, Walker S. In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides. Journal of the American Chemical Society. 127: 14128-9. PMID 16218575 DOI: 10.1021/ja053704n  1
2005 Kelly WL, Hillson NJ, Walsh CT. Excision of the epothilone synthetase B cyclization domain and demonstration of in trans condensation/cyclodehydration activity. Biochemistry. 44: 13385-93. PMID 16201763 DOI: 10.1021/bi051124x  1
2005 Yin J, Lin AJ, Buckett PD, Wessling-Resnick M, Golan DE, Walsh CT. Single-cell FRET imaging of transferrin receptor trafficking dynamics by Sfp-catalyzed, site-specific protein labeling. Chemistry & Biology. 12: 999-1006. PMID 16183024 DOI: 10.1016/j.chembiol.2005.07.006  1
2005 Pacholec M, Hillson NJ, Walsh CT. NovJ/NovK catalyze benzylic oxidation of a beta-hydroxyl tyrosyl-S-pantetheinyl enzyme during aminocoumarin ring formation in novobiocin biosynthesis. Biochemistry. 44: 12819-26. PMID 16171397 DOI: 10.1021/bi051297m  1
2005 Dorrestein PC, Yeh E, Garneau-Tsodikova S, Kelleher NL, Walsh CT. Dichlorination of a pyrrolyl-S-carrier protein by FADH2-dependent halogenase PltA during pyoluteorin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. 102: 13843-8. PMID 16162666 DOI: 10.1073/pnas.0506964102  1
2005 Vaillancourt FH, Yeh E, Vosburg DA, O'Connor SE, Walsh CT. Cryptic chlorination by a non-haem iron enzyme during cyclopropyl amino acid biosynthesis. Nature. 436: 1191-4. PMID 16121186 DOI: 10.1038/nature03797  1
2005 Lin H, Fischbach MA, Liu DR, Walsh CT. In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes. Journal of the American Chemical Society. 127: 11075-84. PMID 16076215 DOI: 10.1021/ja0522027  1
2005 Oberthür M, Leimkuhler C, Kruger RG, Lu W, Walsh CT, Kahne D. A systematic investigation of the synthetic utility of glycopeptide glycosyltransferases. Journal of the American Chemical Society. 127: 10747-52. PMID 16045364 DOI: 10.1021/ja052945s  1
2005 Vaillancourt FH, Yin J, Walsh CT. SyrB2 in syringomycin E biosynthesis is a nonheme FeII alpha-ketoglutarate- and O2-dependent halogenase. Proceedings of the National Academy of Sciences of the United States of America. 102: 10111-6. PMID 16002467 DOI: 10.1073/pnas.0504412102  1
2005 Lu W, Leimkuhler C, Gatto GJ, Kruger RG, Oberthür M, Kahne D, Walsh CT. AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A. Chemistry & Biology. 12: 527-34. PMID 15911373 DOI: 10.1016/j.chembiol.2005.02.016  1
2005 Gatto GJ, McLoughlin SM, Kelleher NL, Walsh CT. Elucidating the substrate specificity and condensation domain activity of FkbP, the FK520 pipecolate-incorporating enzyme. Biochemistry. 44: 5993-6002. PMID 15835888 DOI: 10.1021/bi050230w  1
2005 Pacholec M, Freel Meyers CL, Oberthür M, Kahne D, Walsh CT. Characterization of the aminocoumarin ligase SimL from the simocyclinone pathway and tandem incubation with NovM,P,N from the novobiocin pathway. Biochemistry. 44: 4949-56. PMID 15779922 DOI: 10.1021/bi047303g  1
2005 Yeh E, Garneau S, Walsh CT. Robust in vitro activity of RebF and RebH, a two-component reductase/halogenase, generating 7-chlorotryptophan during rebeccamycin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. 102: 3960-5. PMID 15743914 DOI: 10.1073/pnas.0500755102  1
2005 Garneau S, Dorrestein PC, Kelleher NL, Walsh CT. Characterization of the formation of the pyrrole moiety during clorobiocin and coumermycin A1 biosynthesis. Biochemistry. 44: 2770-80. PMID 15723521 DOI: 10.1021/bi0476329  1
2005 Kruger RG, Lu W, Oberthür M, Tao J, Kahne D, Walsh CT. Tailoring of glycopeptide scaffolds by the acyltransferases from the teicoplanin and A-40,926 biosynthetic operons. Chemistry & Biology. 12: 131-40. PMID 15664522 DOI: 10.1016/j.chembiol.2004.12.005  1
2005 Vosburg DA, Walsh CT. Natural product biosynthetic assembly lines: prospects and challenges for reprogramming. Ernst Schering Research Foundation Workshop. 261-84. PMID 15645725  1
2005 Fischbach MA, Lin H, Liu DR, Walsh CT. In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase. Proceedings of the National Academy of Sciences of the United States of America. 102: 571-6. PMID 15598734 DOI: 10.1073/pnas.0408463102  1
2004 Lin H, Thayer DA, Wong CH, Walsh CT. Macrolactamization of glycosylated peptide thioesters by the thioesterase domain of tyrocidine synthetase. Chemistry & Biology. 11: 1635-42. PMID 15610847 DOI: 10.1016/j.chembiol.2004.09.015  1
2004 Schneider TL, Walsh CT. Portability of oxidase domains in nonribosomal peptide synthetase modules. Biochemistry. 43: 15946-55. PMID 15595851 DOI: 10.1021/bi0481139  1
2004 Yeh E, Lin H, Clugston SL, Kohli RM, Walsh CT. Enhanced macrocyclizing activity of the thioesterase from tyrocidine synthetase in presence of nonionic detergent. Chemistry & Biology. 11: 1573-82. PMID 15556008 DOI: 10.1016/j.chembiol.2004.09.003  1
2004 Liu F, Garneau S, Walsh CT. Hybrid nonribosomal peptide-polyketide interfaces in epothilone biosynthesis: minimal requirements at N and C termini of EpoB for elongation. Chemistry & Biology. 11: 1533-42. PMID 15556004 DOI: 10.1016/j.chembiol.2004.08.017  0.96
2004 Freel Meyers CL, Oberthür M, Heide L, Kahne D, Walsh CT. Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN. Biochemistry. 43: 15022-36. PMID 15554710 DOI: 10.1021/bi048457z  1
2004 Lin H, Walsh CT. A chemoenzymatic approach to glycopeptide antibiotics. Journal of the American Chemical Society. 126: 13998-4003. PMID 15506762 DOI: 10.1021/ja045147v  1
2004 Yin J, Liu F, Schinke M, Daly C, Walsh CT. Phagemid encoded small molecules for high throughput screening of chemical libraries. Journal of the American Chemical Society. 126: 13570-1. PMID 15493886 DOI: 10.1021/ja045127t  1
2004 Tseng CC, Vaillancourt FH, Bruner SD, Walsh CT. DpgC is a metal- and cofactor-free 3,5-dihydroxyphenylacetyl-CoA 1,2-dioxygenase in the vancomycin biosynthetic pathway. Chemistry & Biology. 11: 1195-203. PMID 15380180 DOI: 10.1016/j.chembiol.2004.06.012  1
2004 Reimmann C, Patel HM, Walsh CT, Haas D. PchC thioesterase optimizes nonribosomal biosynthesis of the peptide siderophore pyochelin in Pseudomonas aeruginosa. Journal of Bacteriology. 186: 6367-73. PMID 15375116 DOI: 10.1128/JB.186.19.6367-6373.2004  1
2004 Yeh E, Kohli RM, Bruner SD, Walsh CT. Type II thioesterase restores activity of a NRPS module stalled with an aminoacyl-S-enzyme that cannot be elongated. Chembiochem : a European Journal of Chemical Biology. 5: 1290-3. PMID 15368584 DOI: 10.1002/cbic.200400077  1
2004 Hillson NJ, Balibar CJ, Walsh CT. Catalytically inactive condensation domain C1 is responsible for the dimerization of the VibF subunit of vibriobactin synthetase. Biochemistry. 43: 11344-51. PMID 15366944 DOI: 10.1021/bi0489199  1
2004 Lee HY, Chung HS, Hang C, Khosla C, Walsh CT, Kahne D, Walker S. Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway. Journal of the American Chemical Society. 126: 9924-5. PMID 15303858 DOI: 10.1021/ja048836f  1
2004 Pi N, Meyers CL, Pacholec M, Walsh CT, Leary JA. Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton. Proceedings of the National Academy of Sciences of the United States of America. 101: 10036-41. PMID 15218104 DOI: 10.1073/pnas.0403526101  1
2004 Yin J, Liu F, Li X, Walsh CT. Labeling proteins with small molecules by site-specific posttranslational modification. Journal of the American Chemical Society. 126: 7754-5. PMID 15212504 DOI: 10.1021/ja047749k  1
2004 Merkel AB, Major LL, Errey JC, Burkart MD, Field RA, Walsh CT, Naismith JH. The position of a key tyrosine in dTDP-4-Keto-6-deoxy-D-glucose-5-epimerase (EvaD) alters the substrate profile for this RmlC-like enzyme. The Journal of Biological Chemistry. 279: 32684-91. PMID 15159413 DOI: 10.1074/jbc.M404091200  1
2004 Hicks LM, O'Connor SE, Mazur MT, Walsh CT, Kelleher NL. Mass spectrometric interrogation of thioester-bound intermediates in the initial stages of epothilone biosynthesis. Chemistry & Biology. 11: 327-35. PMID 15123262 DOI: 10.1016/j.chembiol.2004.02.021  1
2004 Mulichak AM, Lu W, Losey HC, Walsh CT, Garavito RM. Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands. Biochemistry. 43: 5170-80. PMID 15122882 DOI: 10.1021/bi036130c  1
2004 Lu W, Leimkuhler C, Oberthür M, Kahne D, Walsh CT. AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A. Biochemistry. 43: 4548-58. PMID 15078101 DOI: 10.1021/bi035945i  1
2004 Lu W, Oberthür M, Leimkuhler C, Tao J, Kahne D, Walsh CT. Characterization of a regiospecific epivancosaminyl transferase GtfA and enzymatic reconstitution of the antibiotic chloroeremomycin. Proceedings of the National Academy of Sciences of the United States of America. 101: 4390-5. PMID 15070728 DOI: 10.1073/pnas.0400277101  1
2004 Tseng CC, McLoughlin SM, Kelleher NL, Walsh CT. Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase. Biochemistry. 43: 970-80. PMID 14744141 DOI: 10.1021/bi035714b  1
2004 Sieber SA, Tao J, Walsh CT, Marahiel MA. Peptidyl thiophenols as substrates for nonribosomal peptide cyclases. Angewandte Chemie (International Ed. in English). 43: 493-8. PMID 14735544 DOI: 10.1002/anie.200352787  0.96
2004 Meyers CLF, Oberthür M, Xu H, Heide L, Kahne D, Walsh CT. Characterization of NovP and NovN: Completion of Novobiocin Biosynthesis by Sequential Tailoring of the Noviosyl Ring Angewandte Chemie - International Edition. 43: 67-70. PMID 14694473 DOI: 10.1002/anie.200352626  1
2004 Couch R, O'Connor SE, Seidle H, Walsh CT, Parry R. Characterization of CmaA, an adenylation-thiolation didomain enzyme involved in the biosynthesis of coronatine. Journal of Bacteriology. 186: 35-42. PMID 14679222 DOI: 10.1128/JB.186.1.35-42.2004  1
2003 Mazur MT, Walsh CT, Kelleher NL. Site-specific observation of acyl intermediate processing in thiotemplate biosynthesis by fourier transform mass spectrometry: the polyketide module of yersiniabactin synthetase. Biochemistry. 42: 13393-400. PMID 14621984 DOI: 10.1021/bi035585z  1
2003 Pfeifer BA, Wang CC, Walsh CT, Khosla C. Biosynthesis of Yersiniabactin, a complex polyketide-nonribosomal peptide, using Escherichia coli as a heterologous host. Applied and Environmental Microbiology. 69: 6698-702. PMID 14602630 DOI: 10.1128/AEM.69.11.6698-6702.2003  0.52
2003 Admiraal SJ, Khosla C, Walsh CT. A Switch for the transfer of substrate between nonribosomal peptide and polyketide modules of the rifamycin synthetase assembly line. Journal of the American Chemical Society. 125: 13664-5. PMID 14599196 DOI: 10.1021/ja0379060  0.52
2003 Clugston SL, Sieber SA, Marahiel MA, Walsh CT. Chirality of peptide bond-forming condensation domains in nonribosomal peptide synthetases: the C5 domain of tyrocidine synthetase is a (D)C(L) catalyst. Biochemistry. 42: 12095-104. PMID 14556641 DOI: 10.1021/bi035090+  1
2003 Sieber SA, Walsh CT, Marahiel MA. Loading peptidyl-coenzyme A onto peptidyl carrier proteins: a novel approach in characterizing macrocyclization by thioesterase domains. Journal of the American Chemical Society. 125: 10862-6. PMID 12952465 DOI: 10.1021/ja0361852  0.76
2003 Patel HM, Tao J, Walsh CT. Epimerization of an L-cysteinyl to a D-cysteinyl residue during thiazoline ring formation in siderophore chain elongation by pyochelin synthetase from Pseudomonas aeruginosa. Biochemistry. 42: 10514-27. PMID 12950179 DOI: 10.1021/bi034840c  1
2003 O'Connor SE, Walsh CT, Liu F. Biosynthesis of epothilone intermediates with alternate starter units: engineering polyketide-nonribosomal interfaces. Angewandte Chemie (International Ed. in English). 42: 3917-21. PMID 12949868 DOI: 10.1002/anie.200352077  0.96
2003 Tao J, Hu S, Pacholec M, Walsh CT. Synthesis of proposed oxidation-cyclization-methylation intermediates of the coumarin antibiotic biosynthetic pathway. Organic Letters. 5: 3233-6. PMID 12943395 DOI: 10.1021/ol035101r  1
2003 Watanabe K, Rude MA, Walsh CT, Khosla C. Engineered biosynthesis of an ansamycin polyketide precursor in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America. 100: 9774-8. PMID 12888623 DOI: 10.1073/pnas.1632167100  0.76
2003 Jakimowicz P, Freel Meyers CL, Walsh CT, Buttner MJ, Lawson DM. Crystallization and preliminary X-ray studies on the putative dTDP sugar epimerase NovW from the novobiocin biosynthetic cluster of Streptomyces spheroides. Acta Crystallographica. Section D, Biological Crystallography. 59: 1507-9. PMID 12876368  0.6
2003 Mulichak AM, Losey HC, Lu W, Wawrzak Z, Walsh CT, Garavito RM. Structure of the TDP-epi-vancosaminyltransferase GtfA from the chloroeremomycin biosynthetic pathway. Proceedings of the National Academy of Sciences of the United States of America. 100: 9238-43. PMID 12874381 DOI: 10.1073/pnas.1233577100  1
2003 Kohli RM, Burke MD, Tao J, Walsh CT. Chemoenzymatic route to macrocyclic hybrid peptide/polyketide-like molecules. Journal of the American Chemical Society. 125: 7160-1. PMID 12797773 DOI: 10.1021/ja0352202  1
2003 Walsh CT, Losey HC, Freel Meyers CL. Antibiotic glycosyltransferases. Biochemical Society Transactions. 31: 487-92. PMID 12773141 DOI: 10.1042/  1
2003 Walsh CT, O'Connor SE, Schneider TL. Polyketide-nonribosomal peptide epothilone antitumor agents: the EpoA, B, C subunits. Journal of Industrial Microbiology & Biotechnology. 30: 448-55. PMID 12707798 DOI: 10.1007/s10295-003-0044-2  1
2003 Meyers CLF, Oberthür M, Anderson JW, Kahne D, Walsh CT. Initial characterization of novobiocic acid noviosyl transferase activity of NovM in biosynthesis of the antibiotic novobiocin Biochemistry. 42: 4179-4189. PMID 12680772 DOI: 10.1021/bi0340088  1
2003 Pojer F, Wemakor E, Kammerer B, Chen H, Walsh CT, Li SM, Heide L. CloQ, a prenyltransferase involved in clorobiocin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. 100: 2316-21. PMID 12618544 DOI: 10.1073/pnas.0337708100  1
2003 Kohli RM, Walsh CT. Enzymology of acyl chain macrocyclization in natural product biosynthesis. Chemical Communications (Cambridge, England). 297-307. PMID 12613585  1
2003 Roche ED, Walsh CT. Dissection of the EntF condensation domain boundary and active site residues in nonribosomal peptide synthesis. Biochemistry. 42: 1334-44. PMID 12564937 DOI: 10.1021/bi026867m  0.68
2003 Hillson NJ, Walsh CT. Dimeric structure of the six-domain VibF subunit of vibriobactin synthetase: mutant domain activity regain and ultracentrifugation studies. Biochemistry. 42: 766-75. PMID 12534289 DOI: 10.1021/bi026903h  1
2002 Losey HC, Jiang J, Biggins JB, Oberthür M, Ye XY, Dong SD, Kahne D, Thorson JS, Walsh CT. Incorporation of glucose analogs by GtfE and GtfD from the vancomycin biosynthetic pathway to generate variant glycopeptides. Chemistry & Biology. 9: 1305-14. PMID 12498883 DOI: 10.1016/S1074-5521(02)00270-3  1
2002 Tseng CC, Bruner SD, Kohli RM, Marahiel MA, Walsh CT, Sieber SA. Characterization of the surfactin synthetase C-terminal thioesterase domain as a cyclic depsipeptide synthase. Biochemistry. 41: 13350-9. PMID 12416979 DOI: 10.1021/bi026592a  1
2002 Sieber SA, Linne U, Hillson NJ, Roche E, Walsh CT, Marahiel MA. Evidence for a monomeric structure of nonribosomal Peptide synthetases. Chemistry & Biology. 9: 997-1008. PMID 12323374 DOI: 10.1016/S1074-5521(02)00214-4  1
2002 Schneider TL, Walsh CT, O'Connor SE. Utilization of alternate substrates by the first three modules of the epothilone synthetase assembly line. Journal of the American Chemical Society. 124: 11272-3. PMID 12236733 DOI: 10.1021/ja0274498  1
2002 Kohli RM, Walsh CT, Burkart MD. Biomimetic synthesis and optimization of cyclic peptide antibiotics. Nature. 418: 658-61. PMID 12167866 DOI: 10.1038/nature00907  1
2002 Dong SD, Oberthür M, Losey HC, Anderson JW, Eggert US, Peczuh MW, Walsh CT, Kahne D. The structural basis for induction of VanB resistance. Journal of the American Chemical Society. 124: 9064-5. PMID 12149006 DOI: 10.1021/ja026342h  1
2002 Luo L, Kohli RM, Onishi M, Linne U, Marahiel MA, Walsh CT. Timing of epimerization and condensation reactions in nonribosomal peptide assembly lines: kinetic analysis of phenylalanine activating elongation modules of tyrocidine synthetase B. Biochemistry. 41: 9184-96. PMID 12119033 DOI: 10.1021/bi026047+  1
2002 Marshall CG, Burkart MD, Meray RK, Walsh CT. Carrier protein recognition in siderophore-producing nonribosomal peptide synthetases. Biochemistry. 41: 8429-37. PMID 12081492 DOI: 10.1021/bi0202575  1
2002 Merkel AB, Temple GK, Burkart MD, Losey HC, Beis K, Walsh CT, Naismith JH. Purification, crystallization and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis, the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathway. Acta Crystallographica. Section D, Biological Crystallography. 58: 1226-8. PMID 12077451 DOI: 10.1107/S0907444902007382  1
2002 Pootoolal J, Thomas MG, Marshall CG, Neu JM, Hubbard BK, Walsh CT, Wright GD. Assembling the glycopeptide antibiotic scaffold: The biosynthesis of A47934 from Streptomyces toyocaensis NRRL15009. Proceedings of the National Academy of Sciences of the United States of America. 99: 8962-7. PMID 12060705 DOI: 10.1073/pnas.102285099  1
2002 Bruner SD, Weber T, Kohli RM, Schwarzer D, Marahiel MA, Walsh CT, Stubbs MT. Structural basis for the cyclization of the lipopeptide antibiotic surfactin by the thioesterase domain SrfTE. Structure (London, England : 1993). 10: 301-10. PMID 12005429 DOI: 10.1016/S0969-2126(02)00716-5  1
2002 O'Connor SE, Chen H, Walsh CT. Enzymatic assembly of epothilones: the EpoC subunit and reconstitution of the EpoA-ACP/B/C polyketide and nonribosomal peptide interfaces. Biochemistry. 41: 5685-94. PMID 11969430  1
2002 Miller DA, Luo L, Hillson N, Keating TA, Walsh CT. Yersiniabactin synthetase: a four-protein assembly line producing the nonribosomal peptide/polyketide hybrid siderophore of Yersinia pestis. Chemistry & Biology. 9: 333-44. PMID 11927258 DOI: 10.1016/S1074-5521(02)00115-1  1
2002 Thomas MG, Burkart MD, Walsh CT. Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis. Chemistry & Biology. 9: 171-84. PMID 11880032 DOI: 10.1016/S1074-5521(02)00100-X  1
2002 Chen H, Hubbard BK, O'Connor SE, Walsh CT. Formation of beta-hydroxy histidine in the biosynthesis of nikkomycin antibiotics. Chemistry & Biology. 9: 103-12. PMID 11841943 DOI: 10.1016/S1074-5521(02)00090-X  1
2002 Kohli RM, Takagi J, Walsh CT. The thioesterase domain from a nonribosomal peptide synthetase as a cyclization catalyst for integrin binding peptides. Proceedings of the National Academy of Sciences of the United States of America. 99: 1247-52. PMID 11805307 DOI: 10.1073/pnas.251668398  1
2002 Marshall CG, Hillson NJ, Walsh CT. Catalytic mapping of the vibriobactin biosynthetic enzyme VibF. Biochemistry. 41: 244-50. PMID 11772022 DOI: 10.1021/bi011852u  1
2001 Keating TA, Ehmann DE, Kohli RM, Marshall CG, Trauger JW, Walsh CT. Chain termination steps in nonribosomal peptide synthetase assembly lines: directed acyl-S-enzyme breakdown in antibiotic and siderophore biosynthesis. Chembiochem : a European Journal of Chemical Biology. 2: 99-107. PMID 11828432 DOI: 10.1002/1439-7633(20010202)2:2<99::AID-CBIC99>3.0.CO;2-3  1
2001 Chen H, Tseng CC, Hubbard BK, Walsh CT. Glycopeptide antibiotic biosynthesis: enzymatic assembly of the dedicated amino acid monomer (S)-3,5-dihydroxyphenylglycine. Proceedings of the National Academy of Sciences of the United States of America. 98: 14901-6. PMID 11752437 DOI: 10.1073/pnas.221582098  1
2001 Luo L, Burkart MD, Stachelhaus T, Walsh CT. Substrate recognition and selection by the initiation module PheATE of gramicidin S synthetase. Journal of the American Chemical Society. 123: 11208-18. PMID 11697963 DOI: 10.1021/ja0166646  1
2001 Forbes AJ, Mazur MT, Patel HM, Walsh CT, Kelleher NL. Toward efficient analysis of >70 kDa proteins with 100% sequence coverage. Proteomics. 1: 927-33. PMID 11683509 DOI: 10.1002/1615-9861(200108)1:8<927::AID-PROT927>3.0.CO;2-T  1
2001 Walsh CT, Chen H, Keating TA, Hubbard BK, Losey HC, Luo L, Marshall CG, Miller DA, Patel HM. Tailoring enzymes that modify nonribosomal peptides during and after chain elongation on NRPS assembly lines. Current Opinion in Chemical Biology. 5: 525-34. PMID 11578925 DOI: 10.1016/S1367-5931(00)00235-0  1
2001 Chen H, Thomas MG, O'Connor SE, Hubbard BK, Burkart MD, Walsh CT. Aminoacyl-S-enzyme intermediates in beta-hydroxylations and alpha,beta-desaturations of amino acids in peptide antibiotics. Biochemistry. 40: 11651-9. PMID 11570865 DOI: 10.1021/bi0115434  1
2001 Marshall CG, Burkart MD, Keating TA, Walsh CT. Heterocycle formation in vibriobactin biosynthesis: alternative substrate utilization and identification of a condensed intermediate. Biochemistry. 40: 10655-63. PMID 11524010 DOI: 10.1021/bi010937s  1
2001 Miller DA, Walsh CT, Luo L. C-methyltransferase and cyclization domain activity at the intraprotein PK/NRP switch point of yersiniabactin synthetase. Journal of the American Chemical Society. 123: 8434-5. PMID 11516308 DOI: 10.1021/ja016398w  1
2001 Mulichak AM, Losey HC, Walsh CT, Garavito RM. Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics. Structure (London, England : 1993). 9: 547-57. PMID 11470430 DOI: 10.1016/S0969-2126(01)00616-5  1
2001 Patel HM, Walsh CT. In vitro reconstitution of the Pseudomonas aeruginosa nonribosomal peptide synthesis of pyochelin: characterization of backbone tailoring thiazoline reductase and N-methyltransferase activities. Biochemistry. 40: 9023-31. PMID 11467965 DOI: 10.1021/bi010519n  1
2001 Zamble DB, Miller DA, Heddle JG, Maxwell A, Walsh CT, Hollfelder F. In vitro characterization of DNA gyrase inhibition by microcin B17 analogs with altered bisheterocyclic sites. Proceedings of the National Academy of Sciences of the United States of America. 98: 7712-7. PMID 11427730 DOI: 10.1073/pnas.141225698  1
2001 Kohli RM, Trauger JW, Schwarzer D, Marahiel MA, Walsh CT. Generality of peptide cyclization catalyzed by isolated thioesterase domains of nonribosomal peptide synthetases. Biochemistry. 40: 7099-108. PMID 11401555  1
2001 Trauger JW, Kohli RM, Walsh CT. Cyclization of backbone-substituted peptides catalyzed by the thioesterase domain from the tyrocidine nonribosomal peptide synthetase. Biochemistry. 40: 7092-8. PMID 11401554  1
2001 Miller DA, Walsh CT. Yersiniabactin synthetase: probing the recognition of carrier protein domains by the catalytic heterocyclization domains, Cy1 and Cy2, in the chain-initiating HWMP2 subunit. Biochemistry. 40: 5313-21. PMID 11318656  1
2001 Losey HC, Peczuh MW, Chen Z, Eggert US, Dong SD, Pelczer I, Kahne D, Walsh CT. Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: novel glycopeptides. Biochemistry. 40: 4745-55. PMID 11294642 DOI: 10.1021/bi010050w  1
2001 Heddle JG, Blance SJ, Zamble DB, Hollfelder F, Miller DA, Wentzell LM, Walsh CT, Maxwell A. The antibiotic microcin B17 is a DNA gyrase poison: characterisation of the mode of inhibition. Journal of Molecular Biology. 307: 1223-34. PMID 11292337 DOI: 10.1006/jmbi.2001.4562  1
2001 Reimmann C, Patel HM, Serino L, Barone M, Walsh CT, Haas D. Essential PchG-dependent reduction in pyochelin biosynthesis of Pseudomonas aeruginosa. Journal of Bacteriology. 183: 813-20. PMID 11208777 DOI: 10.1128/JB.183.3.813-820.2001  1
2001 Suo Z, Tseng CC, Walsh CT. Purification, priming, and catalytic acylation of carrier protein domains in the polyketide synthase and nonribosomal peptidyl synthetase modules of the HMWP1 subunit of yersiniabactin synthetase. Proceedings of the National Academy of Sciences of the United States of America. 98: 99-104. PMID 11134531 DOI: 10.1073/pnas.021537498  1
2000 Suo Z, Chen H, Walsh CT. Acyl-CoA hydrolysis by the high molecular weight protein 1 subunit of yersiniabactin synthetase: mutational evidence for a cascade of four acyl-enzyme intermediates during hydrolytic editing. Proceedings of the National Academy of Sciences of the United States of America. 97: 14188-93. PMID 11106385 DOI: 10.1073/pnas.260495697  1
2000 Chen H, Thomas MG, Hubbard BK, Losey HC, Walsh CT, Burkart MD. Deoxysugars in glycopeptide antibiotics: enzymatic synthesis of TDP-L-epivancosamine in chloroeremomycin biosynthesis. Proceedings of the National Academy of Sciences of the United States of America. 97: 11942-7. PMID 11035791 DOI: 10.1073/pnas.210395097  1
2000 Ehmann DE, Trauger JW, Stachelhaus T, Walsh CT. Aminoacyl-SNACs as small-molecule substrates for the condensation domains of nonribosomal peptide synthetases. Chemistry & Biology. 7: 765-72. PMID 11033080 DOI: 10.1016/S1074-5521(00)00022-3  1
2000 Trauger JW, Kohli RM, Mootz HD, Marahiel MA, Walsh CT. Peptide cyclization catalysed by the thioesterase domain of tyrocidine synthetase. Nature. 407: 215-8. PMID 11001063 DOI: 10.1038/35025116  1
2000 Healy VL, Mullins LS, Li X, Hall SE, Raushel FM, Walsh CT. D-Ala-D-X ligases: evaluation of D-alanyl phosphate intermediate by MIX, PIX and rapid quench studies. Chemistry & Biology. 7: 505-14. PMID 10903933 DOI: 10.1016/S1074-5521(00)00135-6  1
2000 Kuzin AP, Sun T, Jorczak-Baillass J, Healy VL, Walsh CT, Knox JR. Enzymes of vancomycin resistance: the structure of D-alanine-D-lactate ligase of naturally resistant Leuconostoc mesenteroides. Structure (London, England : 1993). 8: 463-70. PMID 10801495 DOI: 10.1016/S0969-2126(00)00129-5  1
2000 Healy VL, Lessard IAD, Roper DI, Knox JR, Walsh CT. Vancomycin resistance in enterococci: Reprogramming of the D-Ala-D-Ala ligases in bacterial peptidoglycan biosynthesis Chemistry and Biology. 7: R109-R119. PMID 10801476 DOI: 10.1016/S1074-5521(00)00116-2  1
2000 Keating TA, Miller DA, Walsh CT. Expression, purification, and characterization of HMWP2, a 229 kDa, six domain protein subunit of Yersiniabactin synthetase. Biochemistry. 39: 4729-39. PMID 10769129 DOI: 10.1021/bi992923g  1
2000 Keating TA, Suo Z, Ehmann DE, Walsh CT. Selectivity of the yersiniabactin synthetase adenylation domain in the two-step process of amino acid activation and transfer to a holo-carrier protein domain. Biochemistry. 39: 2297-306. PMID 10694396 DOI: 10.1021/bi992341z  1
2000 Ehmann DE, Shaw-Reid CA, Losey HC, Walsh CT. The EntF and EntE adenylation domains of Escherichia coli enterobactin synthetase: sequestration and selectivity in acyl-AMP transfers to thiolation domain cosubstrates. Proceedings of the National Academy of Sciences of the United States of America. 97: 2509-14. PMID 10688898 DOI: 10.1073/pnas.040572897  1
2000 Flugel RS, Hwangbo Y, Lambalot RH, Cronan JE, Walsh CT. Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli. The Journal of Biological Chemistry. 275: 959-68. PMID 10625633 DOI: 10.1074/jbc.275.2.959  1
1999 Suo Z, Walsh CT, Miller DA. Tandem heterocyclization activity of the multidomain 230 kDa HMWP2 subunit of yersinia pestis yersiniabactin synthetase: interaction of the 1-1382 and 1383-2035 fragments Biochemistry. 38: 17000. PMID 10606536  1
1999 Kelleher NL, Hendrickson CL, Walsh CT. Posttranslational heterocyclization of cysteine and serine residues in the antibiotic microcin B17: distributivity and directionality. Biochemistry. 38: 15623-30. PMID 10569947 DOI: 10.1021/bi9913698  1
1999 Quadri LE, Keating TA, Patel HM, Walsh CT. Assembly of the Pseudomonas aeruginosa nonribosomal peptide siderophore pyochelin: In vitro reconstitution of aryl-4, 2-bisthiazoline synthetase activity from PchD, PchE, and PchF. Biochemistry. 38: 14941-54. PMID 10555976 DOI: 10.1021/bi991787c  1
1999 Suo Z, Walsh CT, Miller DA. Tandem heterocyclization activity of the multidomain 230 kDa HMWP2 subunit of Yersinia pestis yersiniabactin synthetase: interaction of the 1-1382 and 1383-2035 fragments. Biochemistry. 38: 14023-35. PMID 10529249 DOI: 10.1021/bi991574n  1
1999 Lessard IAD, Healy VL, Park IS, Walsh CT. Determinants for differential effects on D-Ala-D-lactate vs D-Ala-D-Ala formation by the VanA ligase from vancomycin-resistant enterococci Biochemistry. 38: 14006-14022. PMID 10529248 DOI: 10.1021/bi991384c  1
1999 Kelleher NL, Zubarev RA, Bush K, Furie B, Furie BC, McLafferty FW, Walsh CT. Localization of labile posttranslational modifications by electron capture dissociation: the case of gamma-carboxyglutamic acid. Analytical Chemistry. 71: 4250-3. PMID 10517147 DOI: 10.1021/ac990684x  1
1999 Shaw-Reid CA, Kelleher NL, Losey HC, Gehring AM, Berg C, Walsh CT. Assembly line enzymology by multimodular nonribosomal peptide synthetases: the thioesterase domain of E. coli EntF catalyzes both elongation and cyclolactonization. Chemistry & Biology. 6: 385-400. PMID 10375542 DOI: 10.1016/S1074-5521(99)80050-7  1
1999 Ehmann DE, Gehring AM, Walsh CT. Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5. Biochemistry. 38: 6171-7. PMID 10320345 DOI: 10.1021/bi9829940  1
1998 Lessard IA, Pratt SD, McCafferty DG, Bussiere DE, Hutchins C, Wanner BL, Katz L, Walsh CT. Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synechocystis: attributes of catalytic efficiency, stereoselectivity and regulation with implications for function. Chemistry & Biology. 5: 489-504. PMID 9751644  1
1998 Tetaud E, Manai F, Barrett MP, Nadeau K, Walsh CT, Fairlamb AH. Cloning and characterization of the two enzymes responsible for trypanothione biosynthesis in Crithidia fasciculata. The Journal of Biological Chemistry. 273: 19383-90. PMID 9677355  0.44
1998 Healy VL, Park IS, Walsh CT. Active-site mutants of the VanC2 D-alanyl-D-serine ligase, characteristic of one vancomycin-resistant bacterial phenotype, revert towards wild-type D-alanyl-D-alanine ligases. Chemistry & Biology. 5: 197-207. PMID 9545431  1
1998 Skarzynski T, Kim DH, Lees WJ, Walsh CT, Duncan K. Stereochemical course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound to the active site of the C115A mutant of MurA. Biochemistry. 37: 2572-7. PMID 9485407 DOI: 10.1021/bi9722608  1
1997 Walsh CT, Gehring AM, Weinreb PH, Quadri LE, Flugel RS. Post-translational modification of polyketide and nonribosomal peptide synthases. Current Opinion in Chemical Biology. 1: 309-15. PMID 9667867  1
1997 McCafferty DG, Lessard IA, Walsh CT. Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX. Biochemistry. 36: 10498-505. PMID 9265630 DOI: 10.1021/bi970543u  1
1997 Grace MR, Walsh CT, Cole PA. Divalent ion effects and insights into the catalytic mechanism of protein tyrosine kinase Csk. Biochemistry. 36: 1874-81. PMID 9048573 DOI: 10.1021/bi962138t  1
1996 Lambalot RH, Gehring AM, Flugel RS, Zuber P, LaCelle M, Marahiel MA, Reid R, Khosla C, Walsh CT. A new enzyme superfamily - the phosphopantetheinyl transferases. Chemistry & Biology. 3: 923-36. PMID 8939709  1
1996 Walsh CT, Benson TE, Kim DH, Lees WJ. The versatility of phosphoenolpyruvate and its vinyl ether products in biosynthesis. Chemistry & Biology. 3: 83-91. PMID 8807832  1
1996 Kharbanda S, Bharti A, Pei D, Wang J, Pandey P, Ren R, Weichselbaum R, Walsh CT, Kufe D. The stress response to ionizing radiation involoves c-Abl-dependent phosphorylation of SHPTP1. Proceedings of the National Academy of Sciences of the United States of America. 93: 6898-901. PMID 8692915  0.96
1996 Kim DH, Lees WJ, Kempsell KE, Lane WS, Duncan K, Walsh CT. Characterization of a Cys115 to Asp substitution in the Escherichia coli cell wall biosynthetic enzyme UDP-GlcNAc enolpyruvyl transferase (MurA) that confers resistance to inactivation by the antibiotic fosfomycin. Biochemistry. 35: 4923-8. PMID 8664284 DOI: 10.1021/bi952937w  1
1996 Lees WJ, Benson TE, Hogle JM, Walsh CT. (E)-enolbutyryl-UDP-N-acetylglucosamine as a mechanistic probe of UDP-N-acetylenolpyruvylglucosamine reductase (MurB). Biochemistry. 35: 1342-51. PMID 8634262 DOI: 10.1021/bi952287w  1
1996 Kim DH, Tucker-Kellogg GW, Lees WJ, Walsh CT. Analysis of fluoromethyl group chirality establishes a common stereochemical course for the enolpyruvyl transfers catalyzed by EPSP synthase and UDP-GlcNAc enolpyruvyl transferase. Biochemistry. 35: 5435-40. PMID 8611533 DOI: 10.1021/bi952978s  1
1996 Pei D, Wang J, Walsh CT. Differential functions of the two Src homology 2 domains in protein tyrosine phosphatase SH-PTP1. Proceedings of the National Academy of Sciences of the United States of America. 93: 1141-5. PMID 8577729 DOI: 10.1073/pnas.93.3.1141  1
1996 Gehring AM, Lees WJ, Mindiola DJ, Walsh CT, Brown ED. Acetyltransfer precedes uridylyltransfer in the formation of UDP-N-acetylglucosamine in separable active sites of the bifunctional GlmU protein of Escherichia coli. Biochemistry. 35: 579-85. PMID 8555230 DOI: 10.1021/bi952275a  1
1995 Shi Y, Walsh CT. Active site mapping of Escherichia coli D-Ala-D-Ala ligase by structure-based mutagenesis. Biochemistry. 34: 2768-76. PMID 7893688  1
1995 Wu Z, Wright GD, Walsh CT. Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147. Biochemistry. 34: 2455-63. PMID 7873524  0.92
1995 Fan C, Moews PC, Shi Y, Walsh CT, Knox JR. A common fold for peptide synthetases cleaving ATP to ADP: glutathione synthetase and D-alanine:d-alanine ligase of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America. 92: 1172-6. PMID 7862655  1
1995 Cole PA, Grace MR, Phillips RS, Burn P, Walsh CT. The role of the catalytic base in the protein tyrosine kinase Csk. The Journal of Biological Chemistry. 270: 22105-8. PMID 7673185 DOI: 10.1074/jbc.270.38.22105  1
1994 Payne G, Stolz LA, Pei D, Band H, Shoelson SE, Walsh CT. The phosphopeptide-binding specificity of Src family SH2 domains. Chemistry & Biology. 1: 99-105. PMID 9383377 DOI: 10.1016/1074-5521(94)90047-7  1
1994 Fejzo J, Etzkorn FA, Clubb RT, Shi Y, Walsh CT, Wagner G. The mutant Escherichia coli F112W cyclophilin binds cyclosporin A in nearly identical conformation as human cyclophilin. Biochemistry. 33: 5711-20. PMID 8180197  0.8
1994 Shi Y, Brown ED, Walsh CT. Expression of recombinant human casein kinase II and recombinant heat shock protein 90 in Escherichia coli and characterization of their interactions. Proceedings of the National Academy of Sciences of the United States of America. 91: 2767-71. PMID 8146188  1
1994 Nadeau K, Nadler SG, Saulnier M, Tepper MA, Walsh CT. Quantitation of the interaction of the immunosuppressant deoxyspergualin and analogs with Hsc70 and Hsp90. Biochemistry. 33: 2561-7. PMID 8117717  0.44
1994 Etzkorn FA, Chang ZY, Stolz LA, Walsh CT. Cyclophilin residues that affect noncompetitive inhibition of the protein serine phosphatase activity of calcineurin by the cyclophilin.cyclosporin A complex. Biochemistry. 33: 2380-8. PMID 8117697  1
1994 Lorenz U, Ravichandran KS, Pei D, Walsh CT, Burakoff SJ, Neel BG. Lck-dependent tyrosyl phosphorylation of the phosphotyrosine phosphatase SH-PTP1 in murine T cells. Molecular and Cellular Biology. 14: 1824-34. PMID 8114715  0.96
1994 Olson CL, Nadeau KC, Sullivan MA, Winquist AG, Donelson JE, Walsh CT, Engman DM. Molecular and biochemical comparison of the 70-kDa heat shock proteins of Trypanosoma cruzi Journal of Biological Chemistry. 269: 3868-3874. PMID 8106432  1
1994 Ke H, Mayrose D, Belshaw PJ, Alberg DG, Schreiber SL, Chang ZY, Etzkorn FA, Ho S, Walsh CT. Crystal structures of cyclophilin A complexed with cyclosporin A and N-methyl-4-[(E)-2-butenyl]-4,4-dimethylthreonine cyclosporin A. Structure (London, England : 1993). 2: 33-44. PMID 8075981 DOI: 10.1016/S0969-2126(00)00006-X  1
1994 Chu YH, Lees WJ, Stassinopoulos A, Walsh CT. Using affinity capillary electrophoresis to determine binding stoichiometries of protein-ligand interactions. Biochemistry. 33: 10616-21. PMID 8075061  1
1994 Pei D, Lorenz U, Klingmüller U, Neel BG, Walsh CT. Intramolecular regulation of protein tyrosine phosphatase SH-PTP1: a new function for Src homology 2 domains. Biochemistry. 33: 15483-93. PMID 7528537  0.96
1994 Cole PA, Burn P, Takacs B, Walsh CT. Evaluation of the catalytic mechanism of recombinant human Csk (C-terminal Src kinase) using nucleotide analogs and viscosity effects Journal of Biological Chemistry. 269: 30880-30887. PMID 7527038  1
1993 Wright GD, Holman TR, Walsh CT. Purification and characterization of VanR and the cytosolic domain of VanS: a two-component regulatory system required for vancomycin resistance in Enterococcus faecium BM4147. Biochemistry. 32: 5057-63. PMID 8494882  1
1993 Benson TE, Marquardt JL, Marquardt AC, Etzkorn FA, Walsh CT. Overexpression, purification, and mechanistic study of UDP-N-acetylenolpyruvylglucosamine reductase. Biochemistry. 32: 2024-30. PMID 8448160  1
1993 Pei D, Neel BG, Walsh CT. Overexpression, purification, and characterization of SHPTP1, a Src homology 2-containing protein-tyrosine-phosphatase. Proceedings of the National Academy of Sciences of the United States of America. 90: 1092-6. PMID 8430079  1
1993 Nadeau K, Das A, Walsh CT. Hsp90 chaperonins possess ATPase activity and bind heat shock transcription factors and peptidyl prolyl isomerases. The Journal of Biological Chemistry. 268: 1479-87. PMID 8419347  0.44
1993 Wright GD, Walsh CT. Identification of a common protease-sensitive region in D-alanyl-D-alanine and D-alanyl-D-lactate ligases and photoaffinity labeling with 8-azido ATP. Protein Science : a Publication of the Protein Society. 2: 1765-9. PMID 8251948 DOI: 10.1002/pro.5560021020  1
1992 Leichus BN, Bradley M, Nadeau K, Walsh CT, Blanchard JS. Kinetic isotope effect analysis of the reaction catalyzed by Trypanosoma congolense trypanothione reductase Biochemistry. 31: 6414-6420. PMID 1633154  1
1992 Wright GD, Molinas C, Arthur M, Courvalin P, Walsh CT. Characterization of vanY, a DD-carboxypeptidase from vancomycin-resistant Enterococcus faecium BM4147. Antimicrobial Agents and Chemotherapy. 36: 1514-8. PMID 1510448  1
1992 Arthur M, Molinas C, Bugg TD, Wright GD, Walsh CT, Courvalin P. Evidence for in vivo incorporation of D-lactate into peptidoglycan precursors of vancomycin-resistant enterococci. Antimicrobial Agents and Chemotherapy. 36: 867-9. PMID 1503450  1
1992 Zydowsky LD, Etzkorn FA, Chang HY, Ferguson SB, Stolz LA, Ho SI, Walsh CT. Active site mutants of human cyclophilin A separate peptidyl-prolyl isomerase activity from cyclosporin A binding and calcineurin inhibition. Protein Science : a Publication of the Protein Society. 1: 1092-9. PMID 1338979 DOI: 10.1002/pro.5560010903  1
1992 Nadeau K, Sullivan MA, Bradley M, Engman DM, Walsh CT. 83-Kilodalton heat shock proteins of trypanosomes are potent peptide- stimulated ATPases Protein Science. 1: 970-979. PMID 1304385  1
1991 Schiering N, Kabsch W, Moore MJ, Distefano MD, Walsh CT, Pai EF. Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607. Nature. 352: 168-72. PMID 2067577 DOI: 10.1038/352168a0  1
1991 Bugg TD, Wright GD, Dutka-Malen S, Arthur M, Courvalin P, Walsh CT. Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA. Biochemistry. 30: 10408-15. PMID 1931965  1
1990 Miller SM, Massey V, Ballou D, Williams CH, Distefano MD, Moore MJ, Walsh CT. Use of a site-directed triple mutant to trap intermediates: demonstration that the flavin C(4a)-thiol adduct and reduced flavin are kinetically competent intermediates in mercuric ion reductase. Biochemistry. 29: 2831-41. PMID 2189497  1
1990 Distefano MD, Moore MJ, Walsh CT. Active site of mercuric reductase resides at the subunit interface and requires Cys135 and Cys140 from one subunit and Cys558 and Cys559 from the adjacent subunit: evidence from in vivo and in vitro heterodimer formation. Biochemistry. 29: 2703-13. PMID 2189495  1
1989 Shewchuk LM, Verdine GL, Walsh CT. Transcriptional switching by the metalloregulatory MerR protein: initial characterization of DNA and mercury (II) binding activities. Biochemistry. 28: 2331-9. PMID 2719955  1
1989 Moore MJ, Distefano MD, Walsh CT, Schiering N, Pai EF. Purification, crystallization, and preliminary x-ray diffraction studies of the flavoenzyme mercuric ion reductase from Bacillus sp. strain RC607. The Journal of Biological Chemistry. 264: 14386-8. PMID 2668287  1
1989 Miller SM, Moore MJ, Massey V, Williams CH, Distefano MD, Ballou DP, Walsh CT. Evidence for the participation of Cys558 and Cys559 at the active site of mercuric reductase. Biochemistry. 28: 1194-205. PMID 2653437  1
1989 Distefano MD, Au KG, Walsh CT. Mutagenesis of the redox-active disulfide in mercuric ion reductase: catalysis by mutant enzymes restricted to flavin redox chemistry. Biochemistry. 28: 1168-83. PMID 2653436  1
1989 Shewchuk LM, Verdine GL, Nash H, Walsh CT. Mutagenesis of the cysteines in the metalloregulatory protein MerR indicates that a metal-bridged dimer activates transcription. Biochemistry. 28: 6140-5. PMID 2551364  1
1988 Walsh CT, Distefano MD, Moore MJ, Shewchuk LM, Verdine GL. Molecular basis of bacterial resistance to organomercurial and inorganic mercuric salts. Faseb Journal : Official Publication of the Federation of American Societies For Experimental Biology. 2: 124-30. PMID 3277886  1
1987 Wackett LP, Hartwieg EA, King JA, Orme-Johnson WH, Walsh CT. Electron microscopy of nickel-containing methanogenic enzymes: methyl reductase and F420-reducing hydrogenase. Journal of Bacteriology. 169: 718-27. PMID 3804976  1
1987 Wackett LP, Shames SL, Venditti CP, Walsh CT. Bacterial carbon-phosphorus lyase: products, rates, and regulation of phosphonic and phosphinic acid metabolism. Journal of Bacteriology. 169: 710-7. PMID 3804975  1
1987 Neidhart DJ, Distefano MD, Tanizawa K, Soda K, Walsh CT, Petsko GA. X-ray crystallographic studies of the alanine-specific racemase from Bacillus stearothermophilus. Overproduction, crystallization, and preliminary characterization. The Journal of Biological Chemistry. 262: 15323-6. PMID 3680197  1
1987 Wackett LP, Wanner BL, Venditti CP, Walsh CT. Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12. Journal of Bacteriology. 169: 1753-6. PMID 3549702  1
1987 Wackett LP, Honek JF, Begley TP, Wallace V, Orme-Johnson WH, Walsh CT. Substrate analogues as mechanistic probes of methyl-S-coenzyme M reductase. Biochemistry. 26: 6012-8. PMID 3120769  1
1986 Begley TP, Walts AE, Walsh CT. Mechanistic studies of a protonolytic organomercurial cleaving enzyme: bacterial organomercurial lyase. Biochemistry. 25: 7192-200. PMID 3542022  0.56
1986 Begley TP, Walts AE, Walsh CT. Bacterial organomercurial lyase: overproduction, isolation, and characterization. Biochemistry. 25: 7186-92. PMID 3542021  0.56
1985 Schultz PG, Au KG, Walsh CT. Directed mutagenesis of the redox-active disulfide in the flavoenzyme mercuric ion reductase. Biochemistry. 24: 6840-8. PMID 3907703  0.76
1984 Peiser GD, Wang TT, Hoffman NE, Yang SF, Liu HW, Walsh CT. Formation of cyanide from carbon 1 of 1-aminocyclopropane-1-carboxylic acid during its conversion to ethylene. Proceedings of the National Academy of Sciences of the United States of America. 81: 3059-63. PMID 16593463  0.84
1976 Schonbrunn A, Abeles RH, Walsh CT, Ghisla S, Ogata H, Massey V. The structure of the covalent flavin adduct formed between lactate oxidase and the suicide substrate 2-hydroxy-3-butynoate. Biochemistry. 15: 1798-807. PMID 1268196  1
1976 Ghisla S, Ogata H, Massey V, Schonbrunn A, Abeles RH, Walsh CT. Kinetic studies on the inactivation of L-lactate oxidase by [the acetylenic suicide substrate] 2-hydroxy-3-butynoate. Biochemistry. 15: 1791-7. PMID 1268195  1
1973 Abeles RH, Walsh CT. Acetylenic enzyme inactivators. Inactivation of gamma-cystathionase, in vitro and in vivo, by propargylglycine. Journal of the American Chemical Society. 95: 6124-5. PMID 4733835  1
1973 Walsh CT, Krodel E, Massey V, Abeles RH. Studies on the elimination reaction of D-amino acid oxidase with -amino- -chlorobutyrate. Further evidence for abstraction of substrate -hydrogen as a proton. The Journal of Biological Chemistry. 248: 1946-55. PMID 4144104  1
1972 Walsh CT, Schonbrunn A, Lockridge O, Massey V, Abeles RH. Inactivation of a flavoprotein, lactate oxidase, by an acetylenic substrate. The Journal of Biological Chemistry. 247: 6004-6. PMID 5057095  1
1972 Walsh CT, Abeles RH, Kaback HR. Mechanisms of active transport in isolated bacterial membrane vesicles. X. Inactivation of D-lactate dehydrogenase and D-lactate dehydrogenase-coupled transport in Escherichia coli membrane vesicles by an acetylenic substrate. The Journal of Biological Chemistry. 247: 7858-63. PMID 4565667  1
1971 Walsh CT, Spector LB. The glucose-glucose 6-phosphate exchange catalyzed by yeast hexokinase. Archives of Biochemistry and Biophysics. 145: 1-5. PMID 5123138 DOI: 10.1016/0003-9861(71)90002-6  1
1971 Walsh CT, Spector LB. A phosphoenzyme intermediary in phosphoglycerate kinase action. The Journal of Biological Chemistry. 246: 1255-61. PMID 5102139  1
1971 Walsh CT, Schonbrunn A, Abeles RH. Studies on the mechanism of action of D-amino acid oxidase. Evidence for removal of substrate -hydrogen as a proton. The Journal of Biological Chemistry. 246: 6855-66. PMID 4399475  1
1970 Walsh CT, Hildebrand JG, Spector LB. Succinyl phosphate. Its nonenzymatic hydrolysis and reaction with coenzyme A. The Journal of Biological Chemistry. 245: 5699-708. PMID 5472367  1
1969 Walsh CT, Spector LB. Citryl phosphate and the mode of action of the citrate cleavage enzyme. The Journal of Biological Chemistry. 244: 4366-74. PMID 5806583  1
1968 Walsh CT, Spector LB. Citryl 1-phosphate and the citrate cleavage reaction. The Journal of Biological Chemistry. 243: 446-8. PMID 4295092  1
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